##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939617_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1044568 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45400203720581 31.0 31.0 34.0 30.0 34.0 2 31.597162654800837 31.0 31.0 34.0 30.0 34.0 3 31.681955602698913 31.0 31.0 34.0 30.0 34.0 4 35.27038929011802 37.0 35.0 37.0 33.0 37.0 5 35.10187656524037 35.0 35.0 37.0 32.0 37.0 6 35.20854745693914 37.0 35.0 37.0 32.0 37.0 7 35.206602155149305 37.0 35.0 37.0 32.0 37.0 8 35.23017266468052 37.0 35.0 37.0 32.0 37.0 9 36.72310467102189 39.0 35.0 39.0 32.0 39.0 10-11 36.597734661601734 38.5 35.0 39.0 32.0 39.0 12-13 36.562516274670486 38.0 35.0 39.0 32.0 39.0 14-15 37.16743237395747 39.0 36.0 40.0 32.0 41.0 16-17 37.19879940798493 38.5 36.0 40.0 32.0 41.0 18-19 37.504860861140685 39.0 36.0 40.5 32.0 41.0 20-21 37.44358912009558 39.0 36.0 41.0 32.0 41.0 22-23 37.34101178669077 39.0 36.0 40.5 31.5 41.0 24-25 37.14211856001715 39.0 36.0 40.0 31.0 41.0 26-27 36.44454453898645 38.5 35.0 40.0 30.0 41.0 28-29 36.211640601664996 38.0 34.5 40.0 30.0 41.0 30-31 36.23219646782211 38.0 35.0 40.0 30.0 41.0 32-33 36.22557171960083 38.0 35.0 40.0 30.0 41.0 34-35 36.30320620581905 38.0 35.0 40.0 30.0 41.0 36-37 36.01474341545979 38.0 35.0 40.0 30.0 41.0 38-39 35.79083362691563 38.0 34.5 40.0 29.5 41.0 40-41 35.650302325937616 38.0 34.5 40.0 29.0 41.0 42-43 35.754320446347194 38.0 35.0 40.0 29.0 41.0 44-45 35.674581262301736 38.0 35.0 40.0 29.0 41.0 46-47 35.28286765437961 38.0 34.0 40.0 27.0 41.0 48-49 34.96398654754884 38.0 34.0 40.0 26.0 41.0 50-51 34.71140461894295 38.0 33.0 40.0 25.0 41.0 52-53 34.03722974473658 38.0 33.0 40.0 23.5 41.0 54-55 33.85762295992219 37.5 32.5 40.0 23.0 41.0 56-57 32.07620470854937 37.0 30.0 39.5 15.5 40.5 58-59 27.156990258173714 32.5 17.5 38.5 4.5 40.0 60-61 27.708734136982947 32.0 18.5 38.0 6.5 40.0 62-63 30.553502500555254 34.5 28.5 38.0 2.0 40.0 64-65 30.376077957586297 35.0 29.0 38.0 2.0 40.0 66-67 23.79182829648237 26.5 16.5 34.5 2.0 38.0 68-69 16.83626724157738 13.0 7.0 28.0 2.0 34.5 70-71 15.715904086665493 12.5 7.0 25.5 2.0 33.0 72-73 16.681018851812425 13.0 7.0 28.0 2.0 34.0 74-75 17.56254308001011 17.0 7.5 29.0 2.0 34.0 76-77 18.38153619486716 20.5 7.0 29.5 2.0 33.5 78-79 21.16396634781077 25.5 9.0 32.5 2.0 34.5 80-81 24.68366587910026 30.0 19.0 34.0 2.0 35.0 82-83 26.012000654816156 32.0 24.5 34.5 2.0 35.0 84-85 26.094718103560517 33.0 25.0 35.0 2.0 35.0 86-87 25.76190731479425 33.0 23.0 35.0 2.0 35.0 88-89 25.001638955051273 32.0 19.0 34.5 2.0 35.0 90-91 23.551426522734758 31.0 4.5 34.0 2.0 35.0 92-93 21.500068928016177 30.5 2.0 34.0 2.0 35.0 94-95 15.020085815380138 4.5 2.0 31.5 2.0 34.0 96-97 12.457398178002773 2.0 2.0 28.5 2.0 34.0 98-99 12.020064754041861 2.0 2.0 29.0 2.0 34.0 100-101 11.699533682823905 2.0 2.0 29.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3320630372582549 1101 2 0.3724988175844359 1101 3 0.5114714807705418 1101 4 0.29242928858196393 1101 5 0.4705273574242881 1101 6 0.4226981821528426 1101 7 0.43168043468993744 1101 8 0.41034655363768735 1101 9 0.4514782805186712 1101 10-11 0.3988225698877983 1101 12-13 0.396824251346203 1101 14-15 0.5841101233647947 1101 16-17 0.8335281337989002 1101 18-19 0.5545315754543196 1101 20-21 0.6544463557941356 1101 22-23 0.5910077089852734 1101 24-25 0.5843643976317594 1101 26-27 0.3937881098908065 1101 28-29 0.7585733449249972 1101 30-31 0.5682191711519735 1101 32-33 0.7896345040864787 1101 34-35 0.9334443898107736 1101 36-37 0.3932409481234487 1101 38-39 0.5687887472383011 1101 40-41 0.9012275206251488 1101 42-43 0.9490019674190862 1101 44-45 0.6999979995268859 1101 46-47 0.8028184043500133 1101 48-49 0.7597936911886691 1101 50-51 0.6503113028602314 1101 52-53 0.6173693433769927 1101 54-55 0.09419246484936394 1101 56-57 -3.1610513112391523 1101 58-59 -10.650146524815685 1101 60-61 -10.866786261590484 1101 62-63 -4.2762461206601365 1101 64-65 -5.312556018148957 1101 66-67 -9.567597919070916 1101 68-69 -5.124312516346636 1101 70-71 -4.634604073449742 1101 72-73 -4.5194027990847 1101 74-75 -6.3346182352245695 1101 76-77 -7.256025292021295 1101 78-79 -10.446798767247046 1101 80-81 -7.453995126384882 1101 82-83 -4.088100877219539 1101 84-85 -3.2515993675476658 1101 86-87 -3.001443431095531 1101 88-89 -3.111118622940829 1101 90-91 -9.618634142553205 1101 92-93 -15.997944560012183 1101 94-95 -10.83471849649621 1101 96-97 -8.60192786556393 1101 98-99 -8.555409194921602 1101 100-101 -8.37167503392726 1102 1 0.28197701311004764 1102 2 0.3685662850949569 1102 3 0.3952351908691618 1102 4 0.20071756164718835 1102 5 0.2298556009955206 1102 6 0.17688801901419282 1102 7 0.36087180769678895 1102 8 0.34264094056201344 1102 9 0.38296235068980167 1102 10-11 0.4680412862846097 1102 12-13 0.48007949728309995 1102 14-15 0.4388714063535133 1102 16-17 0.5552457748938693 1102 18-19 0.5120014670024418 1102 20-21 0.5131689740005356 1102 22-23 0.6144358480986085 1102 24-25 0.4771356809277165 1102 26-27 0.5989960271847323 1102 28-29 0.46209726271048623 1102 30-31 0.30007345238949057 1102 32-33 0.4337537550619359 1102 34-35 0.28354347119575607 1102 36-37 0.44289685153061953 1102 38-39 0.5956981760993969 1102 40-41 0.724910659892096 1102 42-43 0.45879681518706406 1102 44-45 0.5427178219484645 1102 46-47 0.34562501899255693 1102 48-49 0.37691710511843723 1102 50-51 0.31056776235207906 1102 52-53 0.39345693439362606 1102 54-55 0.10314627295407064 1102 56-57 -2.9460523865410053 1102 58-59 -10.480784562850648 1102 60-61 -10.591716366970065 1102 62-63 -4.642624504020606 1102 64-65 -5.187373600870391 1102 66-67 -6.171397190937949 1102 68-69 -3.9907888289829696 1102 70-71 -4.04607600807139 1102 72-73 -4.249659488998368 1102 74-75 -4.978315046186504 1102 76-77 -6.844831783772193 1102 78-79 -9.075089958988727 1102 80-81 -5.067145684005698 1102 82-83 -2.8246730453353024 1102 84-85 -2.5087443643217604 1102 86-87 -2.5484974880558084 1102 88-89 -2.698408171006818 1102 90-91 -8.316510805430713 1102 92-93 -12.635848182243281 1102 94-95 -9.880255609771579 1102 96-97 -8.372375882400084 1102 98-99 -8.710719265891525 1102 100-101 -8.481590085080907 1103 1 0.3112405917466674 1103 2 0.25925396731868844 1103 3 0.2979880023210306 1103 4 0.2865326086691269 1103 5 0.2153644756109756 1103 6 0.24217891933643898 1103 7 0.24921204635050742 1103 8 0.25129889702561314 1103 9 0.3457932762950193 1103 10-11 0.3966022759741321 1103 12-13 0.32319628123060795 1103 14-15 0.5266594020568789 1103 16-17 0.4757756760272187 1103 18-19 0.35051516072774547 1103 20-21 0.2282920823446446 1103 22-23 0.437572416337062 1103 24-25 0.4370542237224342 1103 26-27 0.44513754240932 1103 28-29 0.3743704649523423 1103 30-31 0.33474893886748447 1103 32-33 0.3859896722273177 1103 34-35 0.40012190321306207 1103 36-37 0.06967707235472886 1103 38-39 0.24660043476028193 1103 40-41 0.5325486201554455 1103 42-43 0.4130212088707097 1103 44-45 0.15489776582904113 1103 46-47 0.0419338694941942 1103 48-49 0.06759528795963377 1103 50-51 0.205452984235734 1103 52-53 0.1516236631716339 1103 54-55 0.08092822193507487 1103 56-57 -2.8041608402607423 1103 58-59 -10.552734621673746 1103 60-61 -10.500343267759977 1103 62-63 -4.822919131023699 1103 64-65 -5.762405478606031 1103 66-67 -6.339982458857072 1103 68-69 -4.325022320152197 1103 70-71 -3.9478980627147617 1103 72-73 -4.466869264593724 1103 74-75 -5.104471870031414 1103 76-77 -6.217215343263904 1103 78-79 -8.617172223641525 1103 80-81 -5.0215554983293025 1103 82-83 -3.237963320890465 1103 84-85 -2.7980192874132968 1103 86-87 -2.54662912392255 1103 88-89 -2.4225785278182457 1103 90-91 -5.076738626199287 1103 92-93 -9.124184949968765 1103 94-95 -7.790960957193228 1103 96-97 -7.48023817423573 1103 98-99 -7.481341603324207 1103 100-101 -7.335253010452297 1104 1 0.12826964493405058 1104 2 0.15595279423242658 1104 3 0.2091029012637584 1104 4 0.23656903411837504 1104 5 0.2685370699746841 1104 6 0.14772959741019065 1104 7 0.17060001163113725 1104 8 0.17518371788712273 1104 9 0.2943594523077735 1104 10-11 0.26012748113969764 1104 12-13 0.18497415448249654 1104 14-15 0.254146839946813 1104 16-17 0.24250569806928723 1104 18-19 0.278450367014095 1104 20-21 0.2961504371596533 1104 22-23 0.33945330823130604 1104 24-25 0.3300595256489345 1104 26-27 0.2268078360544763 1104 28-29 0.5018190645961766 1104 30-31 0.5002247735395287 1104 32-33 0.5038816505339412 1104 34-35 0.5299350437717152 1104 36-37 0.453507519671696 1104 38-39 0.41672090337213064 1104 40-41 0.20378753685636042 1104 42-43 0.4536569886243811 1104 44-45 0.6212847815573568 1104 46-47 0.7077299834902817 1104 48-49 0.7181390234050156 1104 50-51 0.41902792414930445 1104 52-53 0.5672652803370539 1104 54-55 0.5474557247059053 1104 56-57 -2.872621615967148 1104 58-59 -10.052309268938412 1104 60-61 -10.506441806383407 1104 62-63 -4.169607684092778 1104 64-65 -5.332791043542958 1104 66-67 -6.052497324798967 1104 68-69 -3.9193295983928174 1104 70-71 -3.9772758101898855 1104 72-73 -4.207104053077057 1104 74-75 -4.9008357073435285 1104 76-77 -6.3201075083677 1104 78-79 -8.489157005898285 1104 80-81 -4.476036529076122 1104 82-83 -2.659158262422949 1104 84-85 -2.256921962971507 1104 86-87 -2.2798333337082326 1104 88-89 -2.1322961060551293 1104 90-91 -4.360983218421051 1104 92-93 -6.374611859347226 1104 94-95 -7.3382494405246925 1104 96-97 -7.425385572773731 1104 98-99 -7.3335585744702705 1104 100-101 -7.525578706894432 1105 1 0.2317401612836818 1105 2 0.1823913334431495 1105 3 0.20863922966360704 1105 4 0.10027735767937429 1105 5 0.15822874764808859 1105 6 -0.029152245796289833 1105 7 0.01993374408722559 1105 8 0.1172641143764892 1105 9 0.07948508339490701 1105 10-11 0.16998463016884102 1105 12-13 0.2775865774867796 1105 14-15 0.3486151066019545 1105 16-17 0.26149926067189 1105 18-19 0.4050890333680428 1105 20-21 0.31915464337856037 1105 22-23 0.28557713709405164 1105 24-25 0.3405669721685385 1105 26-27 0.3968946915432383 1105 28-29 0.4160560200713661 1105 30-31 0.24181387872277327 1105 32-33 0.44326449669063095 1105 34-35 0.32513106653405544 1105 36-37 -0.06874293088440453 1105 38-39 0.0055957938191042444 1105 40-41 0.06677720801213383 1105 42-43 0.08859043902001673 1105 44-45 0.0796403598121671 1105 46-47 0.0854823103347897 1105 48-49 -0.044556011837116216 1105 50-51 -0.010373252851351822 1105 52-53 0.20319049419689605 1105 54-55 0.16896545795200524 1105 56-57 1.2856350559662921 1105 58-59 -7.993167223413732 1105 60-61 -9.745615730294972 1105 62-63 -3.9961675031296977 1105 64-65 -5.1098340531342465 1105 66-67 -5.78067814283602 1105 68-69 -3.6333186708644547 1105 70-71 -3.580453927976359 1105 72-73 -2.31683722202942 1105 74-75 -4.755513621453957 1105 76-77 -6.2735440015599835 1105 78-79 -8.634393455269358 1105 80-81 -4.477805193208152 1105 82-83 -2.458782696141828 1105 84-85 -1.9642128709963451 1105 86-87 -1.9391269018656452 1105 88-89 -1.877423696752686 1105 90-91 -3.844752397915194 1105 92-93 -5.685946508345511 1105 94-95 -7.061722950294971 1105 96-97 -7.062074186048471 1105 98-99 -7.217307386360013 1105 100-101 -7.0740084726052626 1106 1 0.2816577849328432 1106 2 0.2315021202313403 1106 3 0.17990248416891674 1106 4 0.2391003162316565 1106 5 0.15905345152579287 1106 6 0.26827151846440955 1106 7 0.2188152688396201 1106 8 0.2708057332410476 1106 9 0.3061658054480745 1106 10-11 0.42600957628567926 1106 12-13 0.32685440594836734 1106 14-15 0.25923018335490156 1106 16-17 0.3943259543710411 1106 18-19 0.38941119636789523 1106 20-21 0.470066519490544 1106 22-23 0.5295677070706972 1106 24-25 0.38365281283989106 1106 26-27 0.632902936701278 1106 28-29 0.7285463812860229 1106 30-31 0.6841847566139379 1106 32-33 0.525875469671945 1106 34-35 0.7430236613138703 1106 36-37 0.4764911834569787 1106 38-39 0.4222198304107607 1106 40-41 0.6389961598388894 1106 42-43 0.567358780923243 1106 44-45 0.634014863767355 1106 46-47 0.6389225573886108 1106 48-49 0.4901233974605077 1106 50-51 0.4959883454755456 1106 52-53 0.6592188256432223 1106 54-55 0.40720002743213257 1106 56-57 1.8891904042226635 1106 58-59 -8.647725381493576 1106 60-61 -10.443120084204839 1106 62-63 -4.203326628661475 1106 64-65 -5.388662484635866 1106 66-67 -6.417351417164316 1106 68-69 -3.907215335562615 1106 70-71 -3.5731660658871593 1106 72-73 -4.186625225174016 1106 74-75 -4.807756308201899 1106 76-77 -6.368456885747861 1106 78-79 -8.800247799587458 1106 80-81 -4.440268001602266 1106 82-83 -2.0373166980150437 1106 84-85 -1.6790548137240933 1106 86-87 -1.6397457589031816 1106 88-89 -1.6075367667575406 1106 90-91 -3.8365091206153323 1106 92-93 -5.844676614832931 1106 94-95 -6.680505228471256 1106 96-97 -6.552276478292681 1106 98-99 -6.170166203074514 1106 100-101 -6.716307036097117 1107 1 0.01609120758365279 1107 2 0.06454050115373988 1107 3 -0.024077262005658184 1107 4 0.10600703961107882 1107 5 0.028557163703425203 1107 6 -0.03602723697717636 1107 7 0.09803897456797017 1107 8 0.09782286223442327 1107 9 0.11959912828852026 1107 10-11 0.07736430998395605 1107 12-13 0.12485359211004976 1107 14-15 0.22906826023958615 1107 16-17 0.24642523496867597 1107 18-19 0.15449599678556325 1107 20-21 0.1897174519496474 1107 22-23 0.16727241428084483 1107 24-25 0.24869960854343987 1107 26-27 0.3554762013446151 1107 28-29 0.1756833594889713 1107 30-31 0.16824676955858564 1107 32-33 0.1587931184469653 1107 34-35 0.3247050052219862 1107 36-37 0.32389745768978884 1107 38-39 0.15950936491059053 1107 40-41 0.31835249818065137 1107 42-43 0.054057130981213675 1107 44-45 0.2934302437761076 1107 46-47 0.48809182808198415 1107 48-49 0.09572947667922449 1107 50-51 0.28808720379857533 1107 52-53 0.40489818869956196 1107 54-55 0.06953677991011631 1107 56-57 1.0826958208541377 1107 58-59 -8.95242721723643 1107 60-61 -10.477791708119401 1107 62-63 -4.112830302450508 1107 64-65 -5.740141789341539 1107 66-67 -6.668082719996626 1107 68-69 -3.72748566808467 1107 70-71 -3.8376385681328937 1107 72-73 -2.3344386105111052 1107 74-75 -4.278854490885568 1107 76-77 -6.0840290294440695 1107 78-79 -7.180683625364635 1107 80-81 -2.5777673298017625 1107 82-83 -1.3526848063016068 1107 84-85 -1.2634799828258778 1107 86-87 -1.0487830112007401 1107 88-89 -0.8793568470664894 1107 90-91 -0.6685589950846094 1107 92-93 -3.6235937875893107 1107 94-95 -5.84107610534366 1107 96-97 -5.9146859771678955 1107 98-99 -5.715323673112211 1107 100-101 -6.540936394162945 1108 1 -0.08876208279889397 1108 2 -0.17305013795142443 1108 3 -0.09083507177017225 1108 4 0.006160783905357903 1108 5 -0.08958720951005006 1108 6 -0.10015897960349207 1108 7 -0.2897340568366076 1108 8 -0.19847549718981838 1108 9 -0.09495374001341617 1108 10-11 -0.21945687553415638 1108 12-13 -0.25836201914288637 1108 14-15 -0.14193136415323693 1108 16-17 -0.3357078000009892 1108 18-19 -0.17251700304255024 1108 20-21 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12.053721311049053 2316 100-101 10.718844853990397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 14.0 5 59.0 6 119.0 7 290.0 8 513.0 9 977.0 10 1575.0 11 2639.0 12 4058.0 13 5879.0 14 7508.0 15 9918.0 16 13661.0 17 18566.0 18 20717.0 19 21204.0 20 22814.0 21 23013.0 22 22752.0 23 19391.0 24 17900.0 25 19818.0 26 27345.0 27 41174.0 28 58132.0 29 78699.0 30 94417.0 31 100033.0 32 97189.0 33 86934.0 34 76272.0 35 65932.0 36 49486.0 37 26992.0 38 8133.0 39 442.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.65713995712071 15.473218637457425 12.73901756825209 18.130623837169775 2 12.129474761535935 25.240857561103574 37.90906053344337 24.72060714391712 3 29.18251059772307 27.932228183242003 30.163015382494514 12.722245836540413 4 10.924420430263995 13.397117277190187 43.1009757143623 32.57748657818352 5 11.090681634081225 44.32581926942784 32.60455061807689 11.978948478414042 6 23.57768953289781 37.25004020800943 19.11000528448124 20.062264974611512 7 24.51482335281188 39.27049268214228 18.40311018526319 17.81157377978265 8 19.491032954955593 28.26651170315936 21.3768265094821 30.865628832402948 9 24.048410443360318 25.2396205895643 23.485306844552007 27.226662122523376 10-11 22.48771741045102 22.4349683314059 27.951267892564196 27.126046365578883 12-13 36.78252636496619 20.467982936486663 23.524748987141095 19.224741711406054 14-15 29.857054713441684 22.508270224654293 31.51885410179688 16.115820960107147 16-17 18.65584624457192 26.944583789662328 34.266318707829456 20.133251257936298 18-19 17.164584542965912 34.99489979814593 30.60259926007583 17.23791639881233 20-21 20.282403826270766 30.105459864747914 24.064685113846107 25.547451195135213 22-23 18.678750177107386 27.962192838351697 22.663809972390396 30.695247012150524 24-25 23.405800292561135 27.64798462139372 26.605591976778918 22.34062310926622 26-27 27.572016854871094 21.13268256771123 31.094250283252016 20.201050294165654 28-29 20.181698858961035 25.157427188578986 34.16688167554705 20.493992276912927 30-31 19.173936938213544 32.300590940948034 29.25435354583718 19.271118575001246 32-33 19.30275361104795 30.578988308222538 26.60517214556682 23.513085935162692 34-35 23.011298496581443 23.905824639942097 27.15650593406825 25.92637092940821 36-37 26.20145254828451 23.873081952492463 31.221402686117035 18.704062813106 38-39 28.87981780310526 20.031356419081607 33.032626679885176 18.056199097927955 40-41 20.920451964188672 25.05144451994859 35.62193956622709 18.406163949635648 42-43 17.630383993290604 31.41259210447493 29.797016713592523 21.160007188641945 44-45 19.64309075983743 31.188993022160304 24.918713271217573 24.24920294678469 46-47 20.476176328805668 28.071987096489774 22.142927651597557 29.308908923107 48-49 23.483942752735306 26.05151142113311 25.531356184384606 24.933189641746974 50-51 26.562177304159114 28.13450118380707 27.533133899816526 17.770187612217292 52-53 20.76535586221103 28.886674539318147 31.00385887673392 19.3441107217369 54-55 15.932265418162988 26.48462195448499 33.694797757636806 23.88831486971521 56-57 21.311258354670382 31.29247148025356 25.52814612740987 21.86812403766619 58-59 19.899591982931806 26.951464267360194 28.30881207855107 24.840131671156932 60-61 20.457319479659393 23.572230275937617 31.104780441600294 24.865669802802692 62-63 29.498250847769185 22.37387817781803 27.23024089856832 20.897630075844468 64-65 25.353509355901956 25.785633594496264 30.89046062737576 17.970396422226024 66-67 19.052901128033376 25.704904906859742 32.82452624307032 22.417667722036562 68-69 19.95077463364588 31.16937373298938 28.22196792952585 20.657883703838888 70-71 19.71996022520654 29.954036264441275 26.09235320726156 24.233650303090627 72-73 18.9246814581591 26.55443498794707 26.168691887637134 28.352191666256704 74-75 20.44200920384088 28.996986634146104 27.20641678295701 23.354587379056007 76-77 22.112023772158864 24.348534597605877 29.84312551804824 23.69631611218702 78-79 19.67783868343235 24.26729111748045 32.91559327097527 23.139276928111936 80-81 20.113272522193512 31.819185443642727 28.353860485611037 19.71368154855272 82-83 19.684021729609107 29.82043730144256 26.082854545741746 24.412686423206587 84-85 20.911631326664544 25.60683039000849 26.1574891797271 27.32404910359986 86-87 24.014026898291103 26.586199320386626 27.86347803318991 21.536295748132357 88-89 26.586052683098682 21.95647668195371 29.096472077172685 22.360998557774924 90-91 20.662615446341327 24.190843768537164 32.56657061735837 22.57997016776314 92-93 17.86936559329985 30.818551147069456 30.118744568435783 21.193338691194903 94-95 17.604821795249663 31.35877670065209 26.694811076130186 24.341590427968054 96-97 16.682811629282853 28.08487029613282 25.467275150914077 29.765042923670247 98-99 19.12860844952179 28.021458547709898 25.689673098206573 27.16025990456174 100-101 23.455909726016138 28.03850374932918 25.988628726968578 22.516957797686104 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1517.0 1 1101.5 2 662.0 3 651.5 4 673.5 5 749.0 6 981.0 7 1450.5 8 1772.5 9 1894.0 10 1916.5 11 1849.0 12 1859.0 13 1904.0 14 1943.0 15 1907.5 16 1912.5 17 1949.0 18 1960.0 19 2030.5 20 2100.5 21 2203.5 22 2428.5 23 2665.5 24 2974.0 25 3443.5 26 4010.5 27 4757.0 28 5556.5 29 6292.0 30 7141.0 31 8185.5 32 9598.5 33 11533.5 34 13258.5 35 14661.5 36 16044.5 37 16796.5 38 16809.5 39 16537.0 40 16843.0 41 18015.0 42 20942.0 43 27280.5 44 38662.5 45 55634.0 46 78603.0 47 106995.0 48 135832.0 49 121825.5 50 76583.0 51 47000.5 52 27937.5 53 17033.0 54 11247.5 55 8311.0 56 6721.5 57 5826.5 58 5215.5 59 4592.5 60 3977.5 61 3338.0 62 2601.5 63 1861.0 64 1268.0 65 880.0 66 623.0 67 460.0 68 342.0 69 259.0 70 226.5 71 206.5 72 166.5 73 123.5 74 88.0 75 64.0 76 34.0 77 11.5 78 6.5 79 3.0 80 2.0 81 1.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02307173874750136 2 0.006797068261712019 3 3.829334231950433E-4 4 0.0 5 0.13527123174364905 6 0.0 7 0.0 8 0.0020104004717739774 9 0.0 10-11 0.0 12-13 0.0 14-15 7.180001684907062E-4 16-17 0.0 18-19 1.4360003369814125E-4 20-21 0.0 22-23 3.829334231950433E-4 24-25 0.0 26-27 3.350667452956629E-4 28-29 0.0035900008424535313 30-31 0.01263680296543643 32-33 0.002919867351862205 34-35 0.0046430677562399 36-37 0.10449295785434744 38-39 0.10353562429635983 40-41 0.11593309387229936 42-43 0.12019322820534421 44-45 0.11425776014582106 46-47 0.1082744254083985 48-49 0.10813082537470034 50-51 0.10650335832612143 52-53 0.0833358862228213 54-55 0.0726137503733601 56-57 0.5817716031890695 58-59 1.642784385506736 60-61 0.5466374616109243 62-63 0.005935468059523171 64-65 0.12919216365042774 66-67 1.9568376592045706 68-69 2.9546185600171553 70-71 2.8581193373720044 72-73 2.701403833929433 74-75 2.070281685826102 76-77 1.4805163474278362 78-79 0.9834208974427706 80-81 0.05988121405212489 82-83 2.393333894969021E-4 84-85 9.573335579876083E-5 86-87 3.350667452956629E-4 88-89 1.4360003369814125E-4 90-91 0.2625008616002022 92-93 0.4166315644362071 94-95 2.070664619249297 96-97 2.5073044550474455 98-99 1.4763040797726907 100-101 0.9939994332585337 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1044568.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.955945731825643 #Duplication Level Percentage of deduplicated Percentage of total 1 75.39216144255303 15.79914043795179 2 12.20249260906372 5.114295458170856 3 4.561062028627132 2.867441050542023 4 2.2546432555537317 1.8899272683204276 5 1.2617171413944988 1.3220237971988649 6 0.776253211345616 0.9760272102668466 7 0.5660854137804935 0.8303998647533459 8 0.38935576067363264 0.6527454552840272 9 0.32146058763843216 0.6062859566524589 >10 1.8463080942333892 7.505232548623902 >50 0.22155433791308893 3.2136600356611407 >100 0.15060201895231434 5.988164952243111 >500 0.020599060343021718 3.050871429917018 >1k 0.024718872411626056 12.874976727384682 >5k 0.005950839654650718 9.664961928638828 >10k+ 0.00503532586162753 27.64384587839066 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 60167 5.759988818344042 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 52568 5.032511047629259 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 32498 3.1111425967481297 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 22956 2.197654915716354 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 22789 2.1816674452979608 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21463 2.0547250155088035 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 20148 1.928835652633433 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 17312 1.6573358555881477 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 14593 1.3970368611713169 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 12014 1.1501405365663127 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11667 1.1169210621041425 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 9771 0.9354106195096921 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 9704 0.928996484671175 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 9691 0.9277519510457912 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 9336 0.893766609737231 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 9232 0.88381034073416 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8350 0.7993735209196529 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 8098 0.7752487152583653 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7726 0.7396359069012262 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 6910 0.6615174885694374 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5823 0.5574553308161843 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5692 0.5449142612065466 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 5255 0.5030787847224881 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5165 0.4944627827005997 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4932 0.4721569107994884 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 4903 0.46938064348132436 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4669 0.4469790382244143 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4584 0.43884170298151964 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4469 0.4278323670646621 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 4114 0.3938470257561021 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4021 0.3849438236668173 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 3909 0.3742216878173561 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3849 0.3684776864694304 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3743 0.3583299507547618 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 3593 0.3439699473849477 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3461 0.3313331444195112 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 3271 0.3131438068177467 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3090 0.29581606941817096 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2991 0.28633846719409367 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2788 0.2669045959669452 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2755 0.26374539522558604 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2754 0.26364966186978733 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2742 0.2625008616002022 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2702 0.2586715273682517 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2517 0.240960856545481 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2462 0.23569552197654914 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2433 0.2329192546583851 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2431 0.23272778794678756 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2402 0.22995152062862348 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 2341 0.22411178592489908 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2321 0.22219711880892387 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2307 0.22085685182774126 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2261 0.2164531174609982 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 2211 0.2116664496710602 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2081 0.19922111341722126 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2078 0.198933913349825 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 2045 0.19577471260846588 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 2032 0.194530178983082 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1985 0.19003071126054025 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1963 0.1879245774329675 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1904 0.1822763094408406 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1796 0.17193710701457443 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1688 0.16159790458830828 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1654 0.1583429704911504 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1621 0.1551837697497913 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 1559 0.14924830169026812 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1552 0.1485781681996768 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1539 0.1473336345742929 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1383 0.13239923106968624 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 1370 0.13115469744430233 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1357 0.12991016381891846 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1287 0.12320882891300518 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1140 0.10913602561058734 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1065 0.10195602392568029 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1045 0.10004135680970506 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 9.573335579876083E-5 0.0 8 0.0 0.0 0.0 9.573335579876083E-5 0.0 9 0.0 0.0 0.0 9.573335579876083E-5 0.0 10-11 0.0 0.0 0.0 3.829334231950433E-4 0.0 12-13 0.0 0.0 0.0 6.222668126919454E-4 0.0 14-15 0.0 0.0 0.0 6.701334905913258E-4 0.0 16-17 0.0 0.0 0.0 0.0010052002358869887 0.0 18-19 0.0 0.0 0.0 0.0017710670822770752 0.0 20-21 0.0 0.0 0.0 0.0028720006739628248 0.0 22-23 0.0 0.0 0.0 0.006079068093221313 0.0 24-25 0.0 0.0 0.0 0.008616002021888475 0.0 26-27 0.0 0.0 0.0 0.010961469238958115 0.0 28-29 0.0 0.0 0.0 0.019338137871349685 0.0 30-31 0.0 0.0 0.0 0.03925067587749194 0.0 32-33 0.0 0.0 0.0 0.07936295195717272 0.0 34-35 0.0 0.0 0.0 0.17126697352398312 0.0 36-37 0.0 0.0 0.0 0.29634260287506414 0.0 38-39 0.0 0.0 0.0 0.4180675647731885 0.0 40-41 0.0 0.0 0.0 0.54697252835622 0.0 42-43 0.0 0.0 0.0 0.6747765583475658 0.0 44-45 0.0 0.0 0.0 0.8082288563310382 0.0 46-47 0.0 0.0 0.0 0.9870108982852241 0.0 48-49 0.0 0.0 0.0 1.1778074763921544 0.0 50-51 0.0 0.0 0.0 1.3262420445581333 0.0 52-53 0.0 0.0 0.0 1.448637139946849 0.0 54-55 0.0 0.0 0.0 1.572851169095741 0.0 56-57 0.0 0.0 0.0 1.6832795950096116 0.0 58-59 0.0 0.0 0.0 1.7856664190363865 0.0 60-61 0.0 0.0 0.0 1.90590751391963 0.0 62-63 0.0 0.0 0.0 1.9902007337004388 0.0 64-65 0.0 0.0 0.0 2.0441464796930404 0.0 66-67 0.0 0.0 0.0 2.0879444899709734 0.0 68-69 0.0 0.0 0.0 2.144857969993337 0.0 70-71 0.0 0.0 0.0 2.2403041257247014 0.0 72-73 0.0 0.0 0.0 2.40884269860842 0.0 74-75 0.0 0.0 0.0 2.6021283439661183 0.0 76-77 0.0 0.0 0.0 2.8317447978494457 0.0 78-79 0.0 0.0 0.0 3.225208890182353 0.0 80-81 0.0 0.0 0.0 3.718953672714462 0.0 82-83 0.0 0.0 0.0 4.165789110905179 0.0 84-85 0.0 0.0 0.0 4.7388011120386615 0.0 86-87 0.0 0.0 0.0 5.200714553767682 0.0 88-89 0.0 0.0 0.0 5.470539017086489 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACCG 25 0.001439627 38.9049 66-67 GCGAGTA 75 1.8189894E-11 35.662827 64-65 GGCGATA 35 1.7886414E-4 34.73652 66-67 ATTGCCG 35 1.7886414E-4 34.73652 64-65 CGTCACT 95 9.3598646E-7 34.673832 1 CTGCGCG 205 0.0 34.36357 62-63 TCGCGGT 30 0.0035215626 32.42075 64-65 GATTGGC 145 7.4578566E-10 32.402077 9 GTAGCAA 1800 0.0 31.34006 64-65 CTGCGGG 540 0.0 31.03913 62-63 TGCGTCA 80 1.4988473E-9 30.364365 62-63 ACTGCGC 205 0.0 29.623768 62-63 GCTTATG 240 0.0 29.410837 1 AGTGGTT 550 0.0 29.273405 60-61 ATCGGCG 50 4.2203505E-5 29.178675 64-65 TACCCGG 65 0.006121663 28.954199 2 CTTATGT 265 0.0 28.407894 2 GCAACTC 835 0.0 28.235628 68-69 CGGCTTG 905 0.0 28.134756 74-75 CGGGAAC 35 0.0074825925 27.789215 66-67 >>END_MODULE