FastQCFastQC Report
Fri 27 May 2016
SRR939615_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939615_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989078
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60770.30551843617997887No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA53530.2691196624767857No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50920.2559980051058832No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44100.2217107624738698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41650.20939349789198813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29190.14675140944699No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24860.12498252959411346No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20040.10075019682486057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG95950.027.9969582
GTACATG96650.027.8432921
ACATGGG99650.026.1477573
CATGGGA71850.022.9208744
CATGGGG32750.021.5925254
ATGGGAG29100.020.0604465
GTGTAGC12800.020.0227261
GAGTACT56650.019.4768512-13
TACCTGG13750.019.3296832
ATGGGAT30450.019.326935
AGTACAT69350.019.0255722
AGTACTT60250.019.02198212-13
GTACCTG16600.017.7265011
GTACTTT61900.017.48015814-15
TTTGCGT3953.6561687E-1016.8217183
GTAGCCC12050.016.148653
GTCGACG900.00261810315.83516994-95
TGTACGG1850.003162055515.3924585
CCTACAC8750.015.1876083
ACTTTTT76600.015.05606416-17
Produced by FastQC (version 0.11.5)