##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939615_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1989078 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.096329555703697 31.0 30.0 33.0 26.0 34.0 2 30.230222243672696 31.0 30.0 34.0 26.0 34.0 3 30.270477075308257 31.0 30.0 34.0 26.0 34.0 4 33.837742411308156 35.0 35.0 37.0 28.0 37.0 5 33.826090781759184 35.0 35.0 37.0 28.0 37.0 6 33.93622874517742 35.0 35.0 37.0 28.0 37.0 7 33.74431319435437 35.0 33.0 37.0 28.0 37.0 8 33.74280495787495 35.0 33.0 37.0 28.0 37.0 9 34.982157562448535 37.0 34.0 39.0 28.0 39.0 10-11 34.86416068148157 37.0 34.0 39.0 27.0 39.0 12-13 34.710257214649204 37.0 34.0 39.0 27.0 39.0 14-15 35.360645736366294 38.0 34.0 40.0 27.0 41.0 16-17 35.09897701347056 38.0 33.0 40.0 26.5 41.0 18-19 35.09064626927652 38.0 33.5 40.0 26.0 41.0 20-21 35.22764718125684 38.0 34.0 40.0 26.5 41.0 22-23 35.08503764055507 38.0 34.0 40.0 25.0 41.0 24-25 34.95031416565867 38.0 34.0 40.0 25.0 41.0 26-27 35.07224779520964 38.0 34.0 40.0 25.0 41.0 28-29 34.9474794351956 38.0 34.0 40.0 25.0 41.0 30-31 34.74129621865005 38.0 33.5 40.0 24.0 41.0 32-33 34.5238914713249 38.0 33.0 40.0 24.0 41.0 34-35 34.28779389244665 38.0 33.0 40.0 24.0 41.0 36-37 33.9924645991761 38.0 33.0 40.0 23.0 41.0 38-39 33.81997965891735 38.0 32.5 40.0 22.0 41.0 40-41 33.5365704612891 38.0 32.0 40.0 20.0 41.0 42-43 33.22729978412109 37.0 31.0 40.0 18.0 41.0 44-45 32.96961758161319 37.0 31.0 40.0 16.5 41.0 46-47 32.52343296743516 37.0 30.5 40.0 14.5 41.0 48-49 32.15954527675636 37.0 30.0 39.5 11.0 41.0 50-51 31.55051058832283 36.0 30.0 38.5 9.0 40.5 52-53 31.606975945639135 36.0 30.0 39.0 8.5 40.0 54-55 31.649430037434428 36.0 30.0 39.0 8.0 40.0 56-57 31.511228317843745 36.0 30.0 39.0 7.5 40.0 58-59 31.114192103074892 36.0 29.0 39.0 4.0 40.0 60-61 30.674844576230797 35.0 28.0 39.0 2.0 40.0 62-63 30.241990510176073 35.0 28.0 38.5 2.0 40.0 64-65 29.601997005647846 34.0 27.5 38.0 2.0 40.0 66-67 29.059516016968665 34.0 26.0 38.0 2.0 40.0 68-69 28.539017826349696 34.0 26.0 37.0 2.0 39.0 70-71 27.958701468720683 33.0 25.5 36.5 2.0 39.0 72-73 27.193622120399503 32.5 23.5 36.0 2.0 38.5 74-75 26.71464065260387 32.0 23.0 35.0 2.0 38.0 76-77 26.30680747562438 32.0 22.0 35.0 2.0 37.5 78-79 25.70969388832414 31.5 20.5 35.0 2.0 37.0 80-81 25.015560978503608 31.0 18.5 34.5 2.0 36.0 82-83 24.337841200797556 31.0 15.0 34.0 2.0 36.0 84-85 23.658808503236173 30.0 7.5 34.0 2.0 35.0 86-87 22.96613531495497 29.5 4.0 34.0 2.0 35.0 88-89 22.120065427298478 29.0 2.0 34.0 2.0 35.0 90-91 20.836377960039776 28.0 2.0 33.5 2.0 35.0 92-93 19.165501051240824 25.0 2.0 32.0 2.0 34.0 94-95 18.916732777699014 25.0 2.0 32.0 2.0 34.0 96-97 18.274447256467568 25.0 2.0 32.0 2.0 34.0 98-99 17.29140888391506 22.0 2.0 32.0 2.0 34.0 100-101 15.693918488867705 10.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0749912835245397 1101 2 0.9566549042126553 1101 3 0.9498895710738502 1101 4 1.1693200257447955 1101 5 0.9504387907571328 1101 6 0.8728366125189311 1101 7 0.9882323412275724 1101 8 0.8422211487412241 1101 9 1.0434563136245103 1101 10-11 0.8359729952980572 1101 12-13 0.7718422470240327 1101 14-15 1.0636156668520584 1101 16-17 0.8403627061422227 1101 18-19 1.0723780747419056 1101 20-21 1.2522612547558438 1101 22-23 1.3814915909763315 1101 24-25 1.2639530138440378 1101 26-27 1.15063101530437 1101 28-29 1.2183453448585766 1101 30-31 1.3540261387499015 1101 32-33 1.257958218196741 1101 34-35 1.0911990595456658 1101 36-37 1.2152766336940815 1101 38-39 1.1200129585080134 1101 40-41 1.2908530398730775 1101 42-43 1.283522894346099 1101 44-45 1.130986696804932 1101 46-47 0.9313316582477995 1101 48-49 0.8792670032234966 1101 50-51 0.80313642471123 1101 52-53 1.003195131438435 1101 54-55 1.0613557058270295 1101 56-57 0.9215164610323932 1101 58-59 0.7801566262261019 1101 60-61 0.683706091262529 1101 62-63 0.6575823034785309 1101 64-65 0.5557739913094544 1101 66-67 0.3650500519073674 1101 68-69 0.37968587885211136 1101 70-71 0.42756956448270245 1101 72-73 0.667237101210894 1101 74-75 0.38705859743782156 1101 76-77 -0.21677828482151185 1101 78-79 0.2111673453937044 1101 80-81 0.035417645934334274 1101 82-83 -0.15491003004208892 1101 84-85 -0.35669621040608845 1101 86-87 -0.8746747954646104 1101 88-89 -1.2869021252964679 1101 90-91 -4.354301756344629 1101 92-93 -9.454602553639859 1101 94-95 -7.8643605957744835 1101 96-97 -4.520615364573288 1101 98-99 -3.5000088507403966 1101 100-101 -2.812755533616375 1102 1 0.9502283433821148 1102 2 0.9016755798541354 1102 3 0.8932748817467875 1102 4 1.1103126174115516 1102 5 1.0354322907537323 1102 6 0.9369623481392608 1102 7 0.9259436841511715 1102 8 0.8166864376092917 1102 9 1.0254072799005982 1102 10-11 0.9357263911708458 1102 12-13 0.9371500798839136 1102 14-15 1.1493405332477593 1102 16-17 1.342794406606231 1102 18-19 1.1777104597326797 1102 20-21 1.0975905645427275 1102 22-23 1.1671398247759726 1102 24-25 1.0991278085571565 1102 26-27 0.935569384382049 1102 28-29 1.1366941954933907 1102 30-31 0.9990764526743945 1102 32-33 1.177816826880452 1102 34-35 1.0847937253972262 1102 36-37 1.17850814704709 1102 38-39 0.968460011478868 1102 40-41 1.0438504355404419 1102 42-43 0.8519715498879918 1102 44-45 0.7592024350425888 1102 46-47 0.37346229191923896 1102 48-49 0.5522698067946905 1102 50-51 0.47093118176702475 1102 52-53 0.9040351199791168 1102 54-55 0.9006869758243674 1102 56-57 0.6659493829154108 1102 58-59 0.618325568151775 1102 60-61 0.5211221651119722 1102 62-63 0.26146572293200876 1102 64-65 0.37513515327864866 1102 66-67 0.527553076064116 1102 68-69 0.23737169201460162 1102 70-71 0.3194824898059849 1102 72-73 0.3766983960264234 1102 74-75 0.15140841456528165 1102 76-77 -0.06768101494669665 1102 78-79 -0.11355098263588559 1102 80-81 -0.4037578219823814 1102 82-83 -0.5583121213044855 1102 84-85 -0.9362114233840693 1102 86-87 -1.558243120988248 1102 88-89 -2.524208556061378 1102 90-91 -6.255986188449761 1102 92-93 -10.842679827264323 1102 94-95 -9.49773364275704 1102 96-97 -6.3954319815643785 1102 98-99 -5.139801617885588 1102 100-101 -4.43736780733056 1103 1 0.9362654027228778 1103 2 0.7636424751459323 1103 3 0.7767220823053442 1103 4 0.7139759966332022 1103 5 0.7560040003851327 1103 6 0.5816154543220335 1103 7 0.6904277980774438 1103 8 0.6824085514375895 1103 9 0.867829621001512 1103 10-11 0.7115027574909618 1103 12-13 0.8004338252653511 1103 14-15 0.7825901753663729 1103 16-17 0.829675140687371 1103 18-19 0.8472412083369036 1103 20-21 0.8993636175220345 1103 22-23 0.9398289848173675 1103 24-25 0.6940707825774197 1103 26-27 0.7113416684050264 1103 28-29 0.9794286467658608 1103 30-31 1.033466713352567 1103 32-33 0.9903474750354206 1103 34-35 1.049782460731258 1103 36-37 0.9537094042956369 1103 38-39 0.8326685827194069 1103 40-41 0.7325418786992799 1103 42-43 0.7497015947536738 1103 44-45 0.5327389810035612 1103 46-47 0.7627799594579514 1103 48-49 0.8193737658311946 1103 50-51 0.704376229841639 1103 52-53 0.9155972988074872 1103 54-55 1.0189208157419039 1103 56-57 0.5414942458764038 1103 58-59 0.5417593639310283 1103 60-61 0.46662250876716627 1103 62-63 0.6254245318117206 1103 64-65 0.4924089111345964 1103 66-67 0.519342339474214 1103 68-69 0.6432062100388123 1103 70-71 0.7958767922057319 1103 72-73 0.6940148571844773 1103 74-75 0.5147413949242363 1103 76-77 0.2825402455577972 1103 78-79 0.20187648830434668 1103 80-81 0.2560988720694901 1103 82-83 0.39673832351745375 1103 84-85 0.09783431615745641 1103 86-87 -0.38172978870064256 1103 88-89 -1.0923081997386923 1103 90-91 -2.4930325648296368 1103 92-93 -9.03614817659614 1103 94-95 -5.668426485277852 1103 96-97 -3.2111449658918207 1103 98-99 -2.2797506807138728 1103 100-101 -2.1186591591449044 1104 1 0.8691663358452857 1104 2 0.7770918182919537 1104 3 0.684724008159943 1104 4 0.7484935012758882 1104 5 0.8942640824632235 1104 6 0.6767567982934253 1104 7 0.8853270346136597 1104 8 0.8145350158895255 1104 9 0.9512688763377355 1104 10-11 0.6799658567412479 1104 12-13 0.8366910185615453 1104 14-15 0.8191641029634127 1104 16-17 0.9724552493605927 1104 18-19 0.715492234313011 1104 20-21 0.7829759705036707 1104 22-23 0.6443237918879987 1104 24-25 0.6041415118802149 1104 26-27 0.680571097297431 1104 28-29 0.6271789505464938 1104 30-31 0.8188383574362987 1104 32-33 0.8241306329784237 1104 34-35 0.6867361714403941 1104 36-37 0.7727150672252279 1104 38-39 0.7924168460112355 1104 40-41 0.5018583149511997 1104 42-43 0.5448800802065961 1104 44-45 0.5950743089720163 1104 46-47 0.491446190514651 1104 48-49 0.5132615848008655 1104 50-51 0.5546653372997419 1104 52-53 0.692025870236062 1104 54-55 0.7488096205657122 1104 56-57 0.6747571938095511 1104 58-59 0.5110525294398869 1104 60-61 0.47976211029867954 1104 62-63 0.464374253250611 1104 64-65 0.5769833889758047 1104 66-67 0.41325365845534634 1104 68-69 0.4150849499796614 1104 70-71 0.4473379197477456 1104 72-73 0.4866264535803779 1104 74-75 0.13114291268107436 1104 76-77 0.2143385122886592 1104 78-79 0.12954425545935422 1104 80-81 0.07910274670552653 1104 82-83 -0.0041171915340179055 1104 84-85 -0.016586551852721954 1104 86-87 -0.3682551113959214 1104 88-89 -0.5443147568150621 1104 90-91 -0.867582625585765 1104 92-93 -4.4468524891348284 1104 94-95 -2.2629734509105184 1104 96-97 -1.6079722350758558 1104 98-99 -1.1798118953778811 1104 100-101 -0.9880529734833523 1105 1 0.8769017753169024 1105 2 0.9080853058906797 1105 3 0.8057133389067914 1105 4 0.8095922957888462 1105 5 0.6769083021297462 1105 6 0.7144760722299637 1105 7 0.8579028971604217 1105 8 0.7892068555274179 1105 9 1.1185078029462048 1105 10-11 0.9358609655122052 1105 12-13 0.968830873533598 1105 14-15 1.0789449444949781 1105 16-17 1.122740686522846 1105 18-19 1.1536468230156132 1105 20-21 0.8482357737460191 1105 22-23 0.9405756971117754 1105 24-25 1.0919173651895306 1105 26-27 0.8878645248527377 1105 28-29 0.937971754574022 1105 30-31 1.0338208062839271 1105 32-33 1.1152437455367377 1105 34-35 1.0414528127040725 1105 36-37 0.9256197829427535 1105 38-39 1.1629184394711274 1105 40-41 0.934467663249201 1105 42-43 1.0855063792227995 1105 44-45 0.9996334517367842 1105 46-47 0.917294650170092 1105 48-49 0.8635380154507075 1105 50-51 0.8673331124602477 1105 52-53 0.6963926431333824 1105 54-55 0.8683916260158036 1105 56-57 0.955617662148196 1105 58-59 0.5851377910906237 1105 60-61 0.47311899912598676 1105 62-63 0.42215172311343707 1105 64-65 0.7221542817752145 1105 66-67 0.7923844145405141 1105 68-69 0.7949124406267423 1105 70-71 0.7054511708677857 1105 72-73 0.6731873514603848 1105 74-75 0.5828654659118726 1105 76-77 0.4305994240248374 1105 78-79 0.1777750531130451 1105 80-81 0.17472742916822526 1105 82-83 0.21987223158731695 1105 84-85 -0.033048378096314224 1105 86-87 -0.10507673626313263 1105 88-89 -0.5707333979863698 1105 90-91 -1.1842077237336568 1105 92-93 -7.276603078022177 1105 94-95 -3.301386688174386 1105 96-97 -1.73357867357754 1105 98-99 -1.1164240610977068 1105 100-101 -0.9449695131350992 1106 1 0.9544025709643336 1106 2 1.016910456684812 1106 3 0.9639818206599422 1106 4 1.021484572704459 1106 5 0.8122616643084584 1106 6 0.8908862625791372 1106 7 0.9425005837208005 1106 8 0.8816378804728586 1106 9 1.0064706992544004 1106 10-11 0.8841971625448224 1106 12-13 0.8424034441567798 1106 14-15 0.9068715062372235 1106 16-17 0.8366000596284522 1106 18-19 0.819260835503492 1106 20-21 0.711152588807245 1106 22-23 0.8068098409951432 1106 24-25 0.8730724084575954 1106 26-27 0.8364462833093356 1106 28-29 0.9855959892369697 1106 30-31 0.9869391758886792 1106 32-33 0.8658698442879498 1106 34-35 0.8512116178689624 1106 36-37 0.8947346914811831 1106 38-39 0.7947382745826701 1106 40-41 0.7955222844452479 1106 42-43 0.9601005043137363 1106 44-45 0.6906932635851106 1106 46-47 0.8709072210206017 1106 48-49 0.8507029910508592 1106 50-51 0.9047178856628371 1106 52-53 0.9798062831824872 1106 54-55 1.1070931957145227 1106 56-57 1.0999985442559783 1106 58-59 1.0506767853922696 1106 60-61 1.113431660149871 1106 62-63 1.1941970769113261 1106 64-65 1.0550981583210444 1106 66-67 0.7532248266101078 1106 68-69 0.786996222301088 1106 70-71 0.6893575440036983 1106 72-73 0.941200951348236 1106 74-75 0.8266288687822652 1106 76-77 0.6172779654136598 1106 78-79 0.440752867810545 1106 80-81 0.8289390562293342 1106 82-83 0.7931823806386014 1106 84-85 0.5956525664508483 1106 86-87 0.6050312539314611 1106 88-89 0.7997858756254139 1106 90-91 -0.058279705198859943 1106 92-93 -3.3662498105633993 1106 94-95 -1.0543722604343273 1106 96-97 -0.2614213608199023 1106 98-99 -0.09022902707430802 1106 100-101 0.1976427110404586 1107 1 0.6602313615699984 1107 2 0.7190228715319087 1107 3 0.6271514236763629 1107 4 0.8234204068310689 1107 5 0.6073854985689309 1107 6 0.5736507943497244 1107 7 0.6765611408215833 1107 8 0.58819200462505 1107 9 0.4491540104008749 1107 10-11 0.4759946185553474 1107 12-13 0.6141532247672004 1107 14-15 0.6836688739341525 1107 16-17 0.7425348033144985 1107 18-19 0.6887007971289094 1107 20-21 0.7684645133744183 1107 22-23 0.6394655611135391 1107 24-25 0.6912266867402579 1107 26-27 0.805637618908456 1107 28-29 0.6842713781886545 1107 30-31 0.6836484252044812 1107 32-33 0.5850367774447776 1107 34-35 0.5423008685234123 1107 36-37 0.6288513741992361 1107 38-39 0.5460620489758128 1107 40-41 0.5630066343455908 1107 42-43 0.5083464567619771 1107 44-45 0.36397256603780903 1107 46-47 0.5314259053896535 1107 48-49 0.4108508280183756 1107 50-51 0.6436302293012837 1107 52-53 0.6883245715347535 1107 54-55 0.8531073472718127 1107 56-57 0.7301301454652211 1107 58-59 0.7799249484693753 1107 60-61 0.6893577676953839 1107 62-63 0.5313919999018673 1107 64-65 0.6568658712404876 1107 66-67 0.70219792535773 1107 68-69 0.6381090201503277 1107 70-71 0.707267658420939 1107 72-73 0.9208659313800673 1107 74-75 0.8556481369506983 1107 76-77 0.5296716869673865 1107 78-79 0.5558581422706226 1107 80-81 0.6507017585139572 1107 82-83 0.556908057213743 1107 84-85 0.47123183394995394 1107 86-87 0.3135421053489402 1107 88-89 0.38928171651962984 1107 90-91 -0.05527669416988701 1107 92-93 -3.483264401829464 1107 94-95 -1.1658742237851136 1107 96-97 -0.18772122081960418 1107 98-99 0.06744247329010022 1107 100-101 -0.020247393374106082 1108 1 0.20843083372364646 1108 2 0.20704938547724439 1108 3 0.04297011848526111 1108 4 -0.1591679791578713 1108 5 -0.8975180957545987 1108 6 -0.22948554022614331 1108 7 0.08949487996962091 1108 8 0.04438885841663165 1108 9 0.12250414272151744 1108 10-11 0.1682190392023557 1108 12-13 0.16526716189016355 1108 14-15 0.18423482432126548 1108 16-17 0.16537368406120834 1108 18-19 0.1225808717481911 1108 20-21 0.17535692288462457 1108 22-23 0.036898049313748515 1108 24-25 0.07928766935309994 1108 26-27 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1.1485143540504268 2311 88-89 1.1020428502946338 2311 90-91 1.9942359179876554 2311 92-93 3.0199176395294494 2311 94-95 2.5231434080893465 2311 96-97 2.3210707414462313 2311 98-99 2.131235988956913 2311 100-101 2.160003797971747 2312 1 0.08959584098067452 2312 2 -0.09224524986864324 2312 3 -0.08760092181204726 2312 4 -0.062104071086388046 2312 5 0.03023980608600141 2312 6 0.04777453872292625 2312 7 0.18321685720935932 2312 8 -0.7694799234195315 2312 9 -0.12325026465199329 2312 10-11 -0.06692023102718991 2312 12-13 -0.09079731010325531 2312 14-15 -0.5958078858321016 2312 16-17 -0.21295091310439318 2312 18-19 -0.3625329757710176 2312 20-21 -0.15470843659250022 2312 22-23 -0.2119787291204034 2312 24-25 -0.12971936580419907 2312 26-27 -0.11255682053637628 2312 28-29 -0.07779303824902684 2312 30-31 -0.08028862669068815 2312 32-33 -0.10426339129795537 2312 34-35 -0.3343936138070447 2312 36-37 -0.06426905975889241 2312 38-39 0.011212364218522453 2312 40-41 0.14464542979714423 2312 42-43 0.16845617731210183 2312 44-45 -0.1485671476106134 2312 46-47 -0.1054772455264299 2312 48-49 -0.06723794834572772 2312 50-51 0.12443191059572811 2312 52-53 -0.03048113256506113 2312 54-55 0.05083576435656312 2312 56-57 -0.0753735251446983 2312 58-59 0.013545526638768735 2312 60-61 0.2069516527823403 2312 62-63 0.21450497220486042 2312 64-65 0.12109636750054875 2312 66-67 0.3497709320314435 2312 68-69 0.054249282379284836 2312 70-71 -0.02034648734842648 2312 72-73 -0.0032615016016919185 2312 74-75 0.32315505086968344 2312 76-77 0.5192544786752151 2312 78-79 0.41912875592620935 2312 80-81 0.4887852863880653 2312 82-83 0.7552152080924976 2312 84-85 0.535733709585184 2312 86-87 0.39582051885617986 2312 88-89 0.7301576988338674 2312 90-91 1.2530989803899608 2312 92-93 2.254800171929599 2312 94-95 1.8267697797150646 2312 96-97 1.3597168405543982 2312 98-99 1.2715046312327658 2312 100-101 1.0556445055082477 2313 1 -0.2003844278564202 2313 2 -0.8201049472066977 2313 3 -0.24838919938722626 2313 4 -0.05366283295591501 2313 5 -0.5546218115170163 2313 6 -0.2135801289302961 2313 7 -0.9398346904537291 2313 8 -0.7099618394563834 2313 9 -0.8222531362408816 2313 10-11 -1.6916716095660291 2313 12-13 -0.4385627999469932 2313 14-15 -0.016217747890138412 2313 16-17 -1.4498358557606963 2313 18-19 -0.85893049704368 2313 20-21 -1.1438030052375652 2313 22-23 -0.961403970915228 2313 24-25 -0.13792497509192714 2313 26-27 -0.5615066205821719 2313 28-29 -2.2171494411875585 2313 30-31 -0.5911526578377391 2313 32-33 -0.08328625830639425 2313 34-35 0.21802323343499808 2313 36-37 0.15212432149297683 2313 38-39 -0.13887021598337412 2313 40-41 0.15466604181553123 2313 42-43 0.26344477023354784 2313 44-45 -0.24709325616724698 2313 46-47 -0.4135313476704354 2313 48-49 0.17939366027255943 2313 50-51 0.13735626272651658 2313 52-53 0.37951239314711316 2313 54-55 0.40451267537254054 2313 56-57 0.12936635823003328 2313 58-59 0.20044830661868218 2313 60-61 0.219483583793739 2313 62-63 0.1325750618759507 2313 64-65 0.1827201365050044 2313 66-67 0.31841313734123844 2313 68-69 0.12309034081523862 2313 70-71 0.1488698155249253 2313 72-73 0.3678730843081439 2313 74-75 0.4336002352327384 2313 76-77 0.5180177575716804 2313 78-79 0.5836525214072452 2313 80-81 0.5942397637765033 2313 82-83 0.654505899211717 2313 84-85 0.6544646610852816 2313 86-87 0.703499156348915 2313 88-89 1.1692342099414539 2313 90-91 1.760292486723312 2313 92-93 2.9113887507573573 2313 94-95 1.9500499093769932 2313 96-97 1.3910596607013233 2313 98-99 1.0931261100129568 2313 100-101 1.1212349869838576 2314 1 -0.7306001127188715 2314 2 -0.8121202863972492 2314 3 -2.005237823901126 2314 4 -0.8019290723337065 2314 5 -0.43067503058657763 2314 6 -3.059167411084985 2314 7 -3.8902127690940844 2314 8 -1.7092308874183644 2314 9 -2.253037739438348 2314 10-11 -1.267470075646969 2314 12-13 -2.155099239526429 2314 14-15 -3.0753006371311002 2314 16-17 -2.2337702141072384 2314 18-19 -2.4936417169774288 2314 20-21 -2.27024240463264 2314 22-23 -3.187819065254857 2314 24-25 -1.9308603056406852 2314 26-27 -2.2316708147345565 2314 28-29 -1.5521172144844044 2314 30-31 -1.563014605122774 2314 32-33 -2.6394453393952553 2314 34-35 -1.3524650281234045 2314 36-37 -0.9849039803938169 2314 38-39 -1.1868934048066393 2314 40-41 -1.2683661488801334 2314 42-43 -1.1223295708293932 2314 44-45 -1.5570349240341628 2314 46-47 -1.8847415593944739 2314 48-49 -2.259085743443414 2314 50-51 -1.3294763384545298 2314 52-53 -0.8308244569177035 2314 54-55 -0.7373631347959737 2314 56-57 -0.6818049654052807 2314 58-59 -0.8239206621268167 2314 60-61 -0.5109067373930678 2314 62-63 -0.8522846889522206 2314 64-65 -0.9026599225472758 2314 66-67 -0.8037251674261512 2314 68-69 -0.7968875145786818 2314 70-71 -0.9684299828331362 2314 72-73 -0.7174997732462316 2314 74-75 -0.5094782250870082 2314 76-77 -0.40544202206248414 2314 78-79 -0.4237677220033831 2314 80-81 -0.4548753521676119 2314 82-83 -0.25834247324975834 2314 84-85 -0.5433640872943819 2314 86-87 -0.32857181441300654 2314 88-89 0.05971923082579522 2314 90-91 0.6776850043669818 2314 92-93 1.639028099741946 2314 94-95 0.7515676250618561 2314 96-97 0.5321568089081516 2314 98-99 0.4232769813942028 2314 100-101 0.4201349581778615 2315 1 -6.721916166923265 2315 2 -2.8275095106117014 2315 3 -1.2704310305997488 2315 4 -0.8591476947374019 2315 5 -1.3749468809919279 2315 6 -1.457193921529388 2315 7 -3.1940251248547824 2315 8 -2.7190502554283036 2315 9 -2.980921046142889 2315 10-11 -2.2160765020627338 2315 12-13 -2.6081400999323705 2315 14-15 -4.548899296815101 2315 16-17 -4.331680963263992 2315 18-19 -3.2341700026748015 2315 20-21 -4.3160605743468246 2315 22-23 -5.387673716417648 2315 24-25 -3.5440049443767094 2315 26-27 -4.4534073080181855 2315 28-29 -2.9497313484568295 2315 30-31 -2.62630422285163 2315 32-33 -1.61113930057639 2315 34-35 -2.661370140851968 2315 36-37 -3.259855530781415 2315 38-39 -2.3074904075878706 2315 40-41 -3.35376876146076 2315 42-43 -7.347921691664947 2315 44-45 -4.0499782934643065 2315 46-47 -2.631085647802074 2315 48-49 -5.538608570237898 2315 50-51 -6.590416334904024 2315 52-53 -6.920885259332049 2315 54-55 -4.588236337352338 2315 56-57 -3.925382617929099 2315 58-59 -4.045359430693001 2315 60-61 -3.1684826294576887 2315 62-63 -2.917386544091581 2315 64-65 -1.9833322256421368 2315 66-67 -2.512661347081753 2315 68-69 -1.992206024549688 2315 70-71 -1.5621319030651009 2315 72-73 -2.301715185400795 2315 74-75 -2.7822181061450593 2315 76-77 -2.698229317168707 2315 78-79 -1.5781481587156172 2315 80-81 -1.327129257724156 2315 82-83 -1.8578990298507385 2315 84-85 -1.844411856393144 2315 86-87 -2.298688389358265 2315 88-89 -1.6477560635703234 2315 90-91 -0.7755629688858754 2315 92-93 0.16363754408776643 2315 94-95 -0.026634857333554862 2315 96-97 -0.016938181919709194 2315 98-99 -0.35027285883302994 2315 100-101 -0.27048652830062636 2316 1 -4.463862062160349 2316 2 -3.017874581014354 2316 3 -4.444218834531004 2316 4 -4.461337411362308 2316 5 -3.677296723953429 2316 6 -4.446175494502299 2316 7 -3.266464279787673 2316 8 -1.8064867342369304 2316 9 -1.9182151904300966 2316 10-11 -3.280145784273273 2316 12-13 -3.9403022612997205 2316 14-15 -3.1704243788633093 2316 16-17 -2.952566789804081 2316 18-19 -3.283231300624479 2316 20-21 -5.826738604248838 2316 22-23 -6.679156950940239 2316 24-25 -4.362632460227594 2316 26-27 -4.206664974673089 2316 28-29 -4.718292181760752 2316 30-31 -6.3075208448182 2316 32-33 -4.700729544780081 2316 34-35 -6.276387609179594 2316 36-37 -9.507665740636611 2316 38-39 -6.320003126530128 2316 40-41 -5.444844842791412 2316 42-43 -4.6495414820450165 2316 44-45 -3.648979452137894 2316 46-47 -6.119417672481234 2316 48-49 -3.4473883899082978 2316 50-51 -4.572332278134898 2316 52-53 -5.479192787181386 2316 54-55 -7.284033792975727 2316 56-57 -5.171547452479924 2316 58-59 -6.136300603011879 2316 60-61 -6.672660030029377 2316 62-63 -6.838573214051937 2316 64-65 -5.705753441720056 2316 66-67 -7.145595246812377 2316 68-69 -5.867438715025038 2316 70-71 -6.42333626191278 2316 72-73 -8.475421382245909 2316 74-75 -8.29896005718669 2316 76-77 -7.460595731515081 2316 78-79 -6.451797965799408 2316 80-81 -6.501995598550636 2316 82-83 -5.271354347397878 2316 84-85 -6.58385550192137 2316 86-87 -5.336683104748044 2316 88-89 -5.334047396456654 2316 90-91 -6.005182885720167 2316 92-93 -4.780096943213881 2316 94-95 -4.786834640302814 2316 96-97 -5.458264524981194 2316 98-99 -6.152101078122296 2316 100-101 -5.313405238820442 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11103.0 3 4603.0 4 4613.0 5 5070.0 6 5873.0 7 7140.0 8 8359.0 9 10456.0 10 12482.0 11 15005.0 12 17222.0 13 19340.0 14 21333.0 15 23792.0 16 26472.0 17 28965.0 18 27483.0 19 26499.0 20 28654.0 21 30803.0 22 33858.0 23 37756.0 24 42441.0 25 47297.0 26 53657.0 27 60317.0 28 68029.0 29 77488.0 30 88500.0 31 101429.0 32 115346.0 33 132955.0 34 155709.0 35 184926.0 36 210388.0 37 184255.0 38 58391.0 39 1069.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.89019086717691 18.973454301920647 13.411824375831172 22.724530455071264 2 15.042502098434177 20.885911606102265 40.738507927753474 23.33307836771008 3 20.071224125021132 25.823903206846854 29.290657230501676 24.81421543763033 4 12.179129443224701 16.35785027357437 38.62725196267533 32.8357683205256 5 13.163081738377812 38.64486439837867 34.231188247104676 13.960865616138845 6 30.72160435905886 36.68768882840231 18.419237416261446 14.171469396277391 7 27.972566690064482 33.20411700920566 21.279181227404127 17.544135073325734 8 25.732848214541786 34.60124372489518 20.28522448987807 19.380683570684965 9 26.874906918800995 16.873810070077564 20.546492297908138 35.7047907132133 10-11 24.535290265430174 26.64020491129978 28.088758332823872 20.735746490446168 12-13 26.505390587046033 24.116867293163754 27.891800314934162 21.485941804856044 14-15 23.262842843578238 25.362361137878132 26.86355898643042 24.511237032113208 16-17 21.914259771803195 28.601410864192683 27.371540234084 22.112789129920117 18-19 21.576103802523207 27.915294629127185 29.32951645237386 21.179085115975752 20-21 23.015046472486762 26.74638243287412 28.755145262533883 21.48342583210523 22-23 22.245554789453287 26.876827531366853 28.559993356719026 22.317624322460833 24-25 22.29145571300889 26.80843759257735 29.05951605185867 21.84059064255509 26-27 22.364012829116113 26.674306880874575 29.234013569141254 21.727666720868054 28-29 21.83966401910973 27.272319962142998 29.242570500485172 21.6454455182621 30-31 21.99338344623125 27.15749718772552 28.942526901302696 21.906592464740534 32-33 21.88449939830589 27.13400046162183 28.87613206210299 22.10536807796929 34-35 22.532768808936414 26.8382573170124 28.79922620357008 21.82974767048112 36-37 22.54024211719648 26.663498577380246 29.081826309504873 21.7144329959184 38-39 22.37811834542613 26.887844641017633 29.140052565864 21.593984447692232 40-41 22.2862895361055 26.902634377143148 29.22058425665515 21.5904918300962 42-43 21.731077040780374 27.24015220914664 28.783560002818685 22.245210747254298 44-45 22.12954318399722 27.215443113787526 28.447409413132483 22.20760428908277 46-47 22.377982142758263 27.24407296531193 27.903110595192732 22.47483429673708 48-49 22.453265840323716 27.146820959419106 28.064964874802445 22.33494832545473 50-51 22.315631314491842 27.525482035058285 28.250278070520395 21.908608579929474 52-53 22.338350722394246 27.65537984930208 27.961675412812898 22.044594015490777 54-55 21.93259561928678 27.28646496731095 28.27636405385039 22.504575359551882 56-57 22.30705143533143 27.74651077914396 27.66266879774475 22.28376898777986 58-59 22.48771349895539 27.30768136919202 27.837397121710794 22.36720801014179 60-61 22.274049572550194 27.2024631497724 28.351892776275722 22.171594501401678 62-63 22.482462178074798 27.130139132338677 28.156222828172755 22.23117586141377 64-65 22.21168728653859 27.459544714637023 28.124151532971535 22.20461646585284 66-67 22.0213736462162 27.493560600817652 27.8788046562487 22.606261096717446 68-69 21.94856421822333 27.592236264153847 27.97241531346212 22.486784204160703 70-71 22.15599741778632 27.4225807791188 27.640684714477885 22.78073708861699 72-73 22.06291101724463 27.113525156275607 27.880761541964638 22.942802284515125 74-75 21.963825460452554 27.700017045172732 27.610712425276308 22.72544506909841 76-77 22.202302957910035 27.080225844171146 27.80774340138 22.909727796538814 78-79 22.033257757058998 27.126083130544693 28.03476088984454 22.805898222551775 80-81 22.070679131327896 27.51802179611884 27.668370246467216 22.742928826086047 82-83 22.012652254860043 27.307447678011094 27.804014940578618 22.875885126550248 84-85 21.980944603513528 27.161794151572167 27.62277283004961 23.2344884148647 86-87 21.978569441629862 27.135472193516964 27.84535157267774 23.040606792175442 88-89 22.22471495929285 26.911463518667077 27.68557168532694 23.17824983671313 90-91 21.643482990152417 26.934598291580535 28.206184766769542 23.2157339514975 92-93 21.362361957096958 27.179282280520063 28.50602058873033 22.952335173652653 94-95 21.741677850152204 27.200016114290605 27.706306982271762 23.35199905328543 96-97 21.52293457384268 26.933962418570168 27.74228624771692 23.80081675987023 98-99 21.7438885218634 26.893551385933307 27.420380585423427 23.94217950677987 100-101 21.84423101626907 26.796008171058233 27.699887408661223 23.659873404011474 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1783.0 1 1056.5 2 338.0 3 341.5 4 324.5 5 326.0 6 440.5 7 791.0 8 1253.5 9 1454.5 10 1334.0 11 1207.5 12 1243.5 13 1356.5 14 1549.5 15 1756.0 16 1915.0 17 2100.0 18 2326.5 19 2656.0 20 3017.5 21 3428.0 22 4103.0 23 5024.0 24 6094.0 25 7605.5 26 9889.0 27 12904.0 28 15938.5 29 19114.5 30 22947.0 31 27100.5 32 31497.5 33 36004.5 34 40839.5 35 45772.0 36 50710.5 37 55573.0 38 58858.0 39 60435.5 40 61639.0 41 63451.0 42 64747.0 43 66262.5 44 69069.0 45 71272.5 46 74094.0 47 81016.5 48 96203.5 49 97208.0 50 82176.5 51 73649.5 52 68843.0 53 65037.5 54 60881.0 55 55874.0 56 50597.5 57 45257.5 58 40382.5 59 35607.5 60 30028.0 61 24243.5 62 19020.5 63 14379.5 64 10416.5 65 7523.5 66 5400.0 67 3913.5 68 2853.5 69 2019.5 70 1420.0 71 937.0 72 659.5 73 458.0 74 312.5 75 238.5 76 157.0 77 84.0 78 57.5 79 45.0 80 26.0 81 15.5 82 11.0 83 12.5 84 13.0 85 10.0 86 7.5 87 5.5 88 5.0 89 4.5 90 4.0 91 2.0 92 0.5 93 2.0 94 1.5 95 1.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08420987010061948 2 0.09461670180857663 3 0.09200242524425889 4 0.0938625835688696 5 0.09165050339906228 6 0.0750599021255074 7 0.08612030297454398 8 0.08350602641022624 9 0.07872994422541499 10-11 0.0845366546711592 12-13 0.08438583102321778 14-15 0.08873457953886173 16-17 0.08619571479851469 18-19 0.08878485408817553 20-21 0.09725611564755128 22-23 0.10597874995349604 24-25 0.12887880716593317 26-27 0.13368002662540132 28-29 0.13350406570280302 30-31 0.1355653222246689 32-33 0.13088978913848526 34-35 0.1261388442283309 36-37 0.13081437731451456 38-39 0.122041468459256 40-41 0.11892444640180024 42-43 0.11754189629567065 44-45 0.10924659565889322 46-47 0.12794872800362783 48-49 0.14564536936208636 50-51 0.15439314094268802 52-53 0.1634425598191725 54-55 0.15929490950078376 56-57 0.13086465186382837 58-59 0.12299668489621826 60-61 0.11832115181003461 62-63 0.11565660069640306 64-65 0.10248466877618674 66-67 0.11490248245669601 68-69 0.11316801050536983 70-71 0.12118680112092135 72-73 0.1469776449189021 74-75 0.15979765499392182 76-77 0.165101619946528 78-79 0.16216055881167055 80-81 0.16879679932109248 82-83 0.16816836745466995 84-85 0.14423768198129988 86-87 0.14556995753811566 88-89 0.16635848367937306 90-91 0.25836090892363195 92-93 0.533764890064643 94-95 0.16404585441093814 96-97 0.1784495127893426 98-99 0.19604560504917357 100-101 0.20225451188942817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1989078.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.53193609020737 #Duplication Level Percentage of deduplicated Percentage of total 1 81.84372205592922 53.633775631538484 2 11.019900078085183 14.443107752750988 3 3.299906922251419 6.487478685978385 4 1.3974235946175586 3.6630349477370276 5 0.7073764164120884 2.3177873056018443 6 0.41680196765643585 1.6388303944040536 7 0.2643968787926714 1.2128507554444148 8 0.19336108623054024 1.013706107615427 9 0.126072351603752 0.7435588759235304 >10 0.6724250082983728 7.885158734626906 >50 0.03753298269794458 1.6925234156619402 >100 0.01820375672408252 2.089896318896963 >500 0.0013995733138372887 0.6666279036614592 >1k 0.001244065167855364 1.6698053222418123 >5k 2.3326221897288074E-4 0.841857847916732 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6077 0.30551843617997887 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5353 0.2691196624767857 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5092 0.2559980051058832 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4410 0.2217107624738698 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4165 0.20939349789198813 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2919 0.14675140944699 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2486 0.12498252959411346 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2004 0.10075019682486057 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.0274549313802676E-5 7 0.0 0.0 0.0 0.0 5.0274549313802676E-5 8 0.0 0.0 0.0 0.0 5.0274549313802676E-5 9 0.0 0.0 0.0 0.0 5.0274549313802676E-5 10-11 0.0 0.0 0.0 5.0274549313802676E-5 5.0274549313802676E-5 12-13 0.0 0.0 0.0 5.0274549313802676E-5 4.021963945104214E-4 14-15 0.0 0.0 0.0 5.0274549313802676E-5 5.278827677949281E-4 16-17 0.0 0.0 0.0 1.0054909862760535E-4 7.038436903932375E-4 18-19 0.0 0.0 0.0 1.0054909862760535E-4 7.038436903932375E-4 20-21 0.0 0.0 0.0 1.5082364794140804E-4 7.541182397070402E-4 22-23 0.0 0.0 0.0 4.273336691673228E-4 7.541182397070402E-4 24-25 0.0 0.0 0.0 8.043927890208428E-4 7.541182397070402E-4 26-27 0.0 0.0 0.0 0.0012820010075019683 7.541182397070402E-4 28-29 0.0 0.0 0.0 0.0028405120362298515 7.541182397070402E-4 30-31 0.0 0.0 0.0 0.008119339714179132 7.541182397070402E-4 32-33 0.0 0.0 0.0 0.02098962433851262 8.043927890208428E-4 34-35 0.0 0.0 0.0 0.04230603324756495 8.546673383346455E-4 36-37 0.0 0.0 0.0 0.07400413658991754 8.546673383346455E-4 38-39 0.0 0.0 0.0 0.11814519088743629 8.546673383346455E-4 40-41 0.0 0.0 0.0 0.1690733093423184 8.546673383346455E-4 42-43 0.0 0.0 0.0 0.22012711417048503 9.049418876484482E-4 44-45 0.0 2.5137274656901338E-5 5.0274549313802676E-5 0.27889806231832037 9.049418876484482E-4 46-47 0.0 5.0274549313802676E-5 5.0274549313802676E-5 0.3522486297671584 9.049418876484482E-4 48-49 0.0 5.0274549313802676E-5 5.0274549313802676E-5 0.4324113986480168 9.300791623053495E-4 50-51 0.0 5.0274549313802676E-5 5.0274549313802676E-5 0.5189590352917282 9.552164369622508E-4 52-53 0.0 5.0274549313802676E-5 5.0274549313802676E-5 0.6036465136108287 0.0010557655355898563 54-55 0.0 5.0274549313802676E-5 5.0274549313802676E-5 0.6973834108064139 0.0010557655355898563 56-57 0.0 5.0274549313802676E-5 5.0274549313802676E-5 0.8105011467624699 0.0011311773595605603 58-59 0.0 5.0274549313802676E-5 5.0274549313802676E-5 0.9407625040345327 0.0011563146342174616 60-61 0.0 5.0274549313802676E-5 5.0274549313802676E-5 1.0914353283279992 0.001181451908874363 62-63 0.0 5.0274549313802676E-5 5.0274549313802676E-5 1.2423846626426918 0.0013071382821588696 64-65 0.0 5.0274549313802676E-5 7.541182397070402E-5 1.3904432103718407 0.0013071382821588696 66-67 0.0 5.0274549313802676E-5 1.0054909862760535E-4 1.5586618523758244 0.001332275556815771 68-69 0.0 5.0274549313802676E-5 1.0054909862760535E-4 1.7554113011153911 0.0013574128314726723 70-71 0.0 5.0274549313802676E-5 1.0054909862760535E-4 1.9780521427515663 0.0014328246554433763 72-73 0.0 5.0274549313802676E-5 1.0054909862760535E-4 2.207102989425251 0.0014579619301002776 74-75 0.0 5.0274549313802676E-5 1.0054909862760535E-4 2.4410053301077186 0.0015082364794140803 76-77 0.0 5.0274549313802676E-5 1.0054909862760535E-4 2.6928808221698697 0.001558511028727883 78-79 0.0 5.0274549313802676E-5 1.0054909862760535E-4 2.9844732081899252 0.001633922852698587 80-81 0.0 5.0274549313802676E-5 1.0054909862760535E-4 3.3045209891215928 0.0016841974020123896 82-83 0.0 5.0274549313802676E-5 1.0054909862760535E-4 3.6244179464053197 0.001709334676669291 84-85 0.0 5.0274549313802676E-5 1.0054909862760535E-4 3.948412279458121 0.001709334676669291 86-87 0.0 5.0274549313802676E-5 1.0054909862760535E-4 4.294351453286397 0.001709334676669291 88-89 0.0 5.0274549313802676E-5 1.0054909862760535E-4 4.6640202143907885 0.001709334676669291 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 9595 0.0 27.996958 2 GTACATG 9665 0.0 27.843292 1 ACATGGG 9965 0.0 26.147757 3 CATGGGA 7185 0.0 22.920874 4 CATGGGG 3275 0.0 21.592525 4 ATGGGAG 2910 0.0 20.060446 5 GTGTAGC 1280 0.0 20.022726 1 GAGTACT 5665 0.0 19.47685 12-13 TACCTGG 1375 0.0 19.329683 2 ATGGGAT 3045 0.0 19.32693 5 AGTACAT 6935 0.0 19.025572 2 AGTACTT 6025 0.0 19.021982 12-13 GTACCTG 1660 0.0 17.726501 1 GTACTTT 6190 0.0 17.480158 14-15 TTTGCGT 395 3.6561687E-10 16.821718 3 GTAGCCC 1205 0.0 16.14865 3 GTCGACG 90 0.002618103 15.835169 94-95 TGTACGG 185 0.0031620555 15.392458 5 CCTACAC 875 0.0 15.187608 3 ACTTTTT 7660 0.0 15.056064 16-17 >>END_MODULE