FastQCFastQC Report
Fri 27 May 2016
SRR939615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939615_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989078
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66310.3333705364998255No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55510.2790740232409186No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA53280.26786279874394064No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA50170.25222741390734804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49730.25001533373754076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35140.1766647662887026No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28310.14232724910737538No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT28180.14167367996629593No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22550.11336910870262504No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA21950.11035263574379689No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG21730.10924659565889322No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21210.10663231909457548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95250.024.8681241
TACATGG94100.024.3676552
ACATGGG94650.022.929793
CATGGGA65150.020.0886214
AGTACTT59600.019.56200412-13
GAGTACT56700.019.1944612-13
CATGGGG31900.018.7427274
GTACTTT59250.018.32869714-15
AGTACAT71650.017.4142232
ATGGGAT29450.016.7850935
TACTTTT70800.016.20184714-15
GTATCAA180600.016.1664241
TACACTG16800.015.9729575
TACCTGG14600.015.8022032
CGCGCAA1104.789712E-415.46569566-67
ACTTTTT73250.015.30656716-17
GTACCTG15650.015.045121
CTAGACT10850.014.7526124
AACGCAG199050.014.5956476
ACGATAG1000.004531647514.5823264-65
Produced by FastQC (version 0.11.5)