FastQCFastQC Report
Fri 27 May 2016
SRR939614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010250
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25800.1283422459893048No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23870.11874145006839947No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21830.10859345852505908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG85450.032.1828351
TACATGG96950.026.0658632
ACATGGG97300.024.4186293
GTATAGG5102.4556357E-1023.6154841
CATGGGA69500.022.4939654
GAGTACT55950.021.25815212-13
GTACTTT59500.019.71536414-15
GTGTAGC12250.019.6635061
TAGCCCT14250.019.5550964
GCACCGT3153.5106495E-1019.4788486
AGTACTT59700.019.2903712-13
GCTTATA12250.018.8441931
ATGGGAT32200.018.76135
CATGGGG34600.018.7011074
GTATCAA184150.018.6942561
GTATAGC8552.7284841E-1117.6080381
ATGGGAG28250.017.3749565
CTCCTAC12850.017.1832521
AGTACAT70850.016.8006782
ACTTTTT75300.016.55313116-17
Produced by FastQC (version 0.11.5)