FastQCFastQC Report
Fri 27 May 2016
SRR939614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010250
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66200.32931227459271234No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56580.28145753015794056No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA53840.26782738465365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50800.25270488745180947No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA48650.24200970028603408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36090.17952990921527173No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29240.14545454545454545No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT28490.1417236662106703No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22310.11098122124113916No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA21680.10784728267628405No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG21480.106852381544584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG96050.025.6397911
TACATGG95500.025.138982
ACATGGG96050.023.9620133
CATGGGA70300.021.4011044
GAGTACT61100.020.36542112-13
AGTACTT63550.019.28361512-13
GTACTTT64050.019.09628714-15
TCGCGAA650.006571993718.72084666-67
TACACTG16400.017.829895
ATGGGAG29000.017.2388655
ATGGGAT30150.017.207045
CATGGGG32650.016.7562034
GTATCAA203600.016.6606711
ACTTTTT76900.016.42627716-17
GTGTAGC14100.016.395261
ACCGGGC2607.135599E-616.3150738
TACTTTT76750.015.87496714-15
AGTACAT71450.015.7771632
TACCTGG14350.015.7769322
ATCAACG220250.015.2249013
Produced by FastQC (version 0.11.5)