FastQCFastQC Report
Fri 27 May 2016
SRR939613_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939613_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047872
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71760.3504125257828614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46860.2288228951809488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34720.16954184636539785No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94450.032.8128241
GGTATCA60650.032.083771
TACATGG83800.027.6947382
GTACATG85750.027.563071
TATCAAC115000.026.9493162
ATCAACG113600.026.947213
TCAACGC113000.026.9222894
CAACGCA113900.026.7928945
AACGCAG117600.026.1478756
ACATGGG85250.025.2194823
ACGCAGA134050.022.6913287
CGCAGAG134000.022.523288
GAGTACT61050.020.94898812-13
CATGGGA57450.020.5703534
GCAGAGT137250.020.0196349
AGTACTT64800.019.95636612-13
CATGGGG33050.019.6734944
GTACTTT67600.019.16604214-15
AGAGTAC109150.018.95649310-11
AGTACAT59900.018.3028012
Produced by FastQC (version 0.11.5)