##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939613_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2047872 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.100142001062565 31.0 30.0 33.0 26.0 34.0 2 30.244269661385086 31.0 30.0 34.0 26.0 34.0 3 30.275054300268767 31.0 30.0 34.0 26.0 34.0 4 33.861382449684356 35.0 35.0 37.0 28.0 37.0 5 33.859367187011685 35.0 35.0 37.0 28.0 37.0 6 33.957664834520905 36.0 35.0 37.0 29.0 37.0 7 33.76681013266454 35.0 33.0 37.0 28.0 37.0 8 33.77048565535346 35.0 33.0 37.0 28.0 37.0 9 35.01834147837365 37.0 34.0 39.0 28.0 39.0 10-11 34.905818332395775 37.0 34.0 39.0 27.0 39.0 12-13 34.75261417705794 37.0 34.0 39.0 27.0 39.0 14-15 35.414757123492095 38.0 34.0 40.0 27.0 41.0 16-17 35.146022798299896 38.0 33.0 40.0 26.5 41.0 18-19 35.1313627023564 38.0 33.5 40.0 26.5 41.0 20-21 35.25779247921745 38.0 34.0 40.0 26.5 41.0 22-23 35.102163611788235 38.0 34.0 40.0 25.0 41.0 24-25 34.98337566996375 38.0 34.0 40.0 25.0 41.0 26-27 35.10292318074568 38.0 34.0 40.0 25.0 41.0 28-29 34.98313688550847 38.0 34.0 40.0 25.0 41.0 30-31 34.74379526650103 38.0 33.0 40.0 24.0 41.0 32-33 34.50517146579474 38.0 33.0 40.0 24.0 41.0 34-35 34.27653632648915 38.0 33.0 40.0 24.0 41.0 36-37 33.98083522798299 38.0 33.0 40.0 23.0 41.0 38-39 33.816754416291644 38.0 33.0 40.0 22.0 41.0 40-41 33.53195634297457 38.0 32.0 40.0 20.0 41.0 42-43 33.20862241390087 37.0 31.0 40.0 18.0 41.0 44-45 32.93709250382837 37.0 31.0 40.0 16.5 41.0 46-47 32.47543620890369 37.0 30.5 40.0 14.0 41.0 48-49 32.11191788353959 37.0 30.0 39.5 10.0 41.0 50-51 31.51647393001125 36.0 30.0 38.5 9.0 40.5 52-53 31.580827805644105 36.0 30.0 39.0 8.5 40.0 54-55 31.61814507937996 36.0 30.0 39.0 8.0 40.0 56-57 31.48199301518845 36.0 30.0 39.0 7.0 40.0 58-59 31.09773291494781 36.0 29.0 39.0 4.0 40.0 60-61 30.679627437652353 35.0 28.0 39.0 2.0 40.0 62-63 30.26273956575411 35.0 28.0 38.5 2.0 40.0 64-65 29.64222568598037 34.5 27.5 38.0 2.0 40.0 66-67 29.10354553409588 34.0 26.0 38.0 2.0 40.0 68-69 28.574092033095816 34.0 26.0 37.0 2.0 39.0 70-71 28.011515612303896 33.5 25.5 36.5 2.0 39.0 72-73 27.273317863616477 32.5 24.0 36.0 2.0 39.0 74-75 26.810272077551723 32.0 23.0 35.5 2.0 38.0 76-77 26.412229621773236 32.0 22.0 35.0 2.0 37.5 78-79 25.818037211310084 31.5 20.5 35.0 2.0 37.0 80-81 25.13247117007313 31.0 18.5 34.5 2.0 36.0 82-83 24.45333204419026 31.0 15.5 34.0 2.0 36.0 84-85 23.770708325520346 30.0 8.5 34.0 2.0 35.0 86-87 23.08362143727733 30.0 4.5 34.0 2.0 35.0 88-89 22.26719663143009 29.0 2.0 34.0 2.0 35.0 90-91 21.00706416221326 28.0 2.0 33.5 2.0 35.0 92-93 19.357817529611225 25.0 2.0 32.0 2.0 34.0 94-95 19.086312035127197 25.0 2.0 32.0 2.0 34.0 96-97 18.44393961146009 25.0 2.0 32.0 2.0 34.0 98-99 17.43943517954247 22.0 2.0 32.0 2.0 34.0 100-101 15.809496394305894 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1371926309996283 1101 2 1.1652418237026652 1101 3 1.1683870941958823 1101 4 1.2344596218236603 1101 5 1.1747016142226627 1101 6 1.0497646490504948 1101 7 1.1351761977172998 1101 8 1.1576339701124212 1101 9 1.4352669861976395 1101 10-11 1.3221872362990155 1101 12-13 1.2407365106683415 1101 14-15 1.4187290507216659 1101 16-17 1.1191739617328906 1101 18-19 1.2262559971756488 1101 20-21 1.4632592591196953 1101 22-23 1.5338788906784089 1101 24-25 1.441440294956216 1101 26-27 1.4929228600496174 1101 28-29 1.5343256290084568 1101 30-31 1.3487962395071023 1101 32-33 1.4113656941808443 1101 34-35 1.5308806153592727 1101 36-37 1.4218837715084263 1101 38-39 1.208700003065637 1101 40-41 1.3504868600957352 1101 42-43 1.5882159998950485 1101 44-45 1.5895400986209296 1101 46-47 1.357216532015741 1101 48-49 1.5858941184719626 1101 50-51 1.4508198907671748 1101 52-53 1.5076084855371263 1101 54-55 1.6930484565345658 1101 56-57 1.5998197797092857 1101 58-59 1.442684263572204 1101 60-61 1.1760217020205097 1101 62-63 1.2858667514721986 1101 64-65 1.2672655931881174 1101 66-67 1.4936051909613504 1101 68-69 1.4160211411139407 1101 70-71 1.403756045857694 1101 72-73 1.2658505864193081 1101 74-75 1.0396043320244814 1101 76-77 0.67354561728542 1101 78-79 0.7030001799286758 1101 80-81 0.5293644598313385 1101 82-83 0.26568973231230686 1101 84-85 -0.05577586230461051 1101 86-87 -0.5123484508143221 1101 88-89 -1.0951652976899346 1101 90-91 -4.312070591133985 1101 92-93 -9.170309797901968 1101 94-95 -7.444806782422592 1101 96-97 -4.093326449344392 1101 98-99 -3.12965344704957 1101 100-101 -2.760025462726759 1102 1 0.9902364463042765 1102 2 0.867998462387682 1102 3 0.8565785878666681 1102 4 1.0703742680782469 1102 5 0.9179899865745398 1102 6 0.8707749588245122 1102 7 1.0967560132564174 1102 8 0.9220365172756786 1102 9 1.0425596685863212 1102 10-11 0.8268623155726686 1102 12-13 0.7899747623767297 1102 14-15 1.0369995167279242 1102 16-17 1.297885877199782 1102 18-19 1.2371970624937205 1102 20-21 1.1316429590926589 1102 22-23 1.1974284536318507 1102 24-25 1.1989096697538955 1102 26-27 1.1965072247061315 1102 28-29 1.2852051393081894 1102 30-31 1.192950768552926 1102 32-33 1.1179603007730847 1102 34-35 0.9742830614237121 1102 36-37 1.054075412711022 1102 38-39 1.0810456240214776 1102 40-41 1.1813212085336247 1102 42-43 1.1257002793585187 1102 44-45 1.0459314373174422 1102 46-47 0.8503162908604835 1102 48-49 0.8078000913043724 1102 50-51 0.6907953833604488 1102 52-53 0.8886675400886617 1102 54-55 0.7372544899933295 1102 56-57 0.8831115044583697 1102 58-59 0.8471127868741632 1102 60-61 0.8343332266598118 1102 62-63 0.685889737457007 1102 64-65 0.46730902945453323 1102 66-67 0.490656859701037 1102 68-69 0.4040667824542048 1102 70-71 0.48458652487837384 1102 72-73 0.4903698154469822 1102 74-75 0.29022780216394395 1102 76-77 0.2336284458656266 1102 78-79 0.1286235595073073 1102 80-81 -0.015370942833389023 1102 82-83 0.09567824261302249 1102 84-85 -0.12200595991600949 1102 86-87 -0.6282847526328155 1102 88-89 -1.1925050157263293 1102 90-91 -5.719890370475117 1102 92-93 -10.685651954823797 1102 94-95 -9.630962304015092 1102 96-97 -6.25908487323032 1102 98-99 -4.791273076421971 1102 100-101 -4.056472218064004 1103 1 0.9579828859177155 1103 2 0.9392974888275027 1103 3 0.9761566862149138 1103 4 1.083055611882827 1103 5 0.9597290458499543 1103 6 0.8137801322215381 1103 7 0.9758192269000716 1103 8 1.023423591199247 1103 9 1.0290029645235563 1103 10-11 0.8855195083334948 1103 12-13 0.9567223246403387 1103 14-15 1.0410774987194884 1103 16-17 0.9950388465966995 1103 18-19 1.00096627732907 1103 20-21 0.9411588897287402 1103 22-23 0.8522894777240779 1103 24-25 0.7461664651119051 1103 26-27 0.6547230292678279 1103 28-29 0.815655171146453 1103 30-31 0.7245340633138042 1103 32-33 1.0134907977311869 1103 34-35 1.109167177841769 1103 36-37 0.9767916890926713 1103 38-39 1.0135128459609817 1103 40-41 0.9729909652586599 1103 42-43 0.8435450467602337 1103 44-45 0.8923532775942462 1103 46-47 1.061419275473277 1103 48-49 0.9039310398798293 1103 50-51 0.9705466357930668 1103 52-53 0.9947178317475505 1103 54-55 1.191747775829274 1103 56-57 0.9256862860019055 1103 58-59 0.6783673420464105 1103 60-61 0.7532631799381271 1103 62-63 0.8121282967822907 1103 64-65 0.6948409380365419 1103 66-67 0.7204783097354301 1103 68-69 0.8077710165587284 1103 70-71 0.9490788133713366 1103 72-73 0.7796234448803645 1103 74-75 0.6946794041780642 1103 76-77 0.504889752282633 1103 78-79 0.4697184375118013 1103 80-81 0.377892644686014 1103 82-83 0.23076106043937727 1103 84-85 0.04823997463271468 1103 86-87 -0.1794424376719057 1103 88-89 -0.5518990915090463 1103 90-91 -1.958698903639327 1103 92-93 -8.891174933350236 1103 94-95 -5.313337207797069 1103 96-97 -2.427535206828761 1103 98-99 -1.7394365588367684 1103 100-101 -1.2784126127560906 1104 1 0.7790527985404374 1104 2 0.7859141844759172 1104 3 0.6989028600838161 1104 4 0.7800465501355021 1104 5 0.6370077134814807 1104 6 0.6521699709677975 1104 7 0.9465148969185435 1104 8 0.9592882374238769 1104 9 0.8872163206102641 1104 10-11 0.7631583777212043 1104 12-13 0.7130693279993352 1104 14-15 0.7997677036078699 1104 16-17 0.8626077277571298 1104 18-19 0.9066220389370656 1104 20-21 0.8580003469527497 1104 22-23 0.890015076350231 1104 24-25 0.770249219710287 1104 26-27 0.7701393404130243 1104 28-29 0.8881270084454158 1104 30-31 1.0086895063025665 1104 32-33 0.788702760533738 1104 34-35 0.7524977750098358 1104 36-37 0.6628647879078358 1104 38-39 0.3939652852883171 1104 40-41 0.4256101234558187 1104 42-43 0.6294793571925865 1104 44-45 0.5829021667471395 1104 46-47 0.37560485228211604 1104 48-49 0.20196154092209184 1104 50-51 0.3451775234986876 1104 52-53 0.5361404936763279 1104 54-55 0.46577112683967314 1104 56-57 0.421044165617829 1104 58-59 0.16520296691815162 1104 60-61 0.15217933447386045 1104 62-63 0.19176906708481667 1104 64-65 0.5727314626242261 1104 66-67 0.4400377511256899 1104 68-69 0.2896594026172501 1104 70-71 0.4997846360030209 1104 72-73 0.5385599046425362 1104 74-75 0.24568926517366663 1104 76-77 0.20804948313037386 1104 78-79 -0.005741400262397889 1104 80-81 0.09299519688016389 1104 82-83 0.21241993263526027 1104 84-85 -0.029355152112938043 1104 86-87 -0.34132747552819254 1104 88-89 -0.4715735598469344 1104 90-91 -0.8204413728844706 1104 92-93 -4.348926021515959 1104 94-95 -1.984688477413755 1104 96-97 -1.117267566663294 1104 98-99 -0.9348873240629878 1104 100-101 -1.0465868704812582 1105 1 1.0690493069388545 1105 2 0.8701727922289706 1105 3 0.9542917597623557 1105 4 0.7639766926331717 1105 5 0.7863707880436266 1105 6 0.794505056469589 1105 7 0.957526283360302 1105 8 0.9770588975700605 1105 9 1.1841092197114662 1105 10-11 0.8918152997417437 1105 12-13 0.8546082434378448 1105 14-15 1.0582545772023337 1105 16-17 1.228457211074378 1105 18-19 1.0356066833190383 1105 20-21 0.9802966109390567 1105 22-23 0.9221749556193473 1105 24-25 0.9640177190963328 1105 26-27 0.9662018963290535 1105 28-29 0.9846027195275937 1105 30-31 1.114732311852194 1105 32-33 1.2582411368434876 1105 34-35 1.1182773201677065 1105 36-37 0.9181047904124497 1105 38-39 0.9431206679264577 1105 40-41 0.6450018843373542 1105 42-43 0.8742145208043652 1105 44-45 0.8269745628242404 1105 46-47 0.7269353222898971 1105 48-49 0.7517451995899123 1105 50-51 0.5409457872812276 1105 52-53 0.5735210071434054 1105 54-55 0.7781326699789908 1105 56-57 0.51708846756814 1105 58-59 0.41214303978543754 1105 60-61 0.29761738189384346 1105 62-63 0.44153670046877025 1105 64-65 0.4582542636315914 1105 66-67 0.6191336704092016 1105 68-69 0.6863762358513519 1105 70-71 0.5261642696714013 1105 72-73 0.6936719171316419 1105 74-75 0.6123061579454152 1105 76-77 0.507116196068722 1105 78-79 0.3489274229192887 1105 80-81 0.15044828047377834 1105 82-83 0.24864673179175512 1105 84-85 0.029726355027861473 1105 86-87 -0.13240638041760278 1105 88-89 -0.396350134490266 1105 90-91 -1.594460461013984 1105 92-93 -7.209944996088822 1105 94-95 -3.205869763702797 1105 96-97 -1.4776697269989114 1105 98-99 -1.1035086508503653 1105 100-101 -0.852000657107256 1106 1 0.898439875488684 1106 2 0.8952940020486047 1106 3 0.9008998131909749 1106 4 0.8784979969551756 1106 5 0.818085395616265 1106 6 0.7869680546744249 1106 7 0.9767800174934749 1106 8 0.8575701852086368 1106 9 0.9770730757489119 1106 10-11 0.897811728337679 1106 12-13 1.0595495533012667 1106 14-15 1.0929733143440146 1106 16-17 1.1718158877951126 1106 18-19 1.1332306608940073 1106 20-21 1.0207188524529514 1106 22-23 1.2010145639905474 1106 24-25 1.2184991876167999 1106 26-27 1.1036464140439932 1106 28-29 1.2331908034742796 1106 30-31 1.2597423429688845 1106 32-33 1.1403947403438153 1106 34-35 1.3466723249342323 1106 36-37 1.468408610565497 1106 38-39 1.2933464774845476 1106 40-41 1.297283676494125 1106 42-43 1.5482798902284216 1106 44-45 1.2599757181949869 1106 46-47 1.4116563427262108 1106 48-49 1.2712922128630737 1106 50-51 1.2107754676980598 1106 52-53 1.348282509328861 1106 54-55 1.3919709971024723 1106 56-57 1.3723941936215311 1106 58-59 1.3731630596654796 1106 60-61 1.1727851685579331 1106 62-63 1.0701850917871418 1106 64-65 1.1364158399708835 1106 66-67 1.016349920179394 1106 68-69 1.1443410182202491 1106 70-71 0.9104022963937588 1106 72-73 1.2001929119666634 1106 74-75 1.1577829985944028 1106 76-77 1.1525077621698685 1106 78-79 0.9678755978097229 1106 80-81 1.1061158081672993 1106 82-83 0.9034251232591863 1106 84-85 1.0240827131989505 1106 86-87 0.8415289240260222 1106 88-89 0.6726711413201301 1106 90-91 0.3484907718349852 1106 92-93 -3.2998588910369193 1106 94-95 -0.8704527169799618 1106 96-97 0.07641643578603663 1106 98-99 0.31663436147540125 1106 100-101 0.3921384331571769 1107 1 0.6807515433277871 1107 2 0.7017042626369729 1107 3 0.6886915976251373 1107 4 0.5666416057127535 1107 5 0.7660173045507008 1107 6 0.6794467797081367 1107 7 0.6491015412554688 1107 8 0.45791274487142175 1107 9 0.5581125520488399 1107 10-11 0.4018852692583792 1107 12-13 0.5698350724504877 1107 14-15 0.7233232484904377 1107 16-17 0.7703797722857431 1107 18-19 0.5570013591468097 1107 20-21 0.555826329499638 1107 22-23 0.6171848652130691 1107 24-25 0.6142079737274386 1107 26-27 0.6244347528624132 1107 28-29 0.6578283760028398 1107 30-31 0.6432782263030674 1107 32-33 0.4377231893232292 1107 34-35 0.43964400905865375 1107 36-37 0.48847323506507934 1107 38-39 0.44099316057309323 1107 40-41 0.38869014508949107 1107 42-43 0.2735128685095489 1107 44-45 0.09635785425327725 1107 46-47 0.25670629042044624 1107 48-49 0.33230256367713196 1107 50-51 0.11761651172754384 1107 52-53 0.41256458891462344 1107 54-55 0.4077930820980562 1107 56-57 0.2888284678377886 1107 58-59 0.1635830514064338 1107 60-61 0.07219434857960394 1107 62-63 0.2761083524634991 1107 64-65 0.2684985915471607 1107 66-67 0.21814284151311014 1107 68-69 0.2614252619189976 1107 70-71 0.15735287292101674 1107 72-73 0.3596111843914507 1107 74-75 0.26964167108492276 1107 76-77 0.10559063618863718 1107 78-79 -0.17383506823557582 1107 80-81 -0.1464309259932719 1107 82-83 -0.05189034774637946 1107 84-85 -0.2804940524288817 1107 86-87 -0.2988852714825292 1107 88-89 -0.5576759063491075 1107 90-91 -0.8972638952959997 1107 92-93 -3.884533466340688 1107 94-95 -1.6323237035545866 1107 96-97 -0.7545509949745934 1107 98-99 -0.5003430825676745 1107 100-101 -0.36982031163494433 1108 1 0.31171765000111407 1108 2 0.4815533649145358 1108 3 0.12980282877769156 1108 4 -0.22926496227984927 1108 5 -0.6450717958362091 1108 6 -0.1063570346837821 1108 7 0.19106474780252825 1108 8 0.3639122755843971 1108 9 0.43787210153330136 1108 10-11 0.22419450746037484 1108 12-13 0.26849414386989423 1108 14-15 0.33685990014441813 1108 16-17 0.3309908076207009 1108 18-19 0.30016082557759205 1108 20-21 0.3559052051689662 1108 22-23 0.156139275160605 1108 24-25 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0.46224704415296003 2311 78-79 0.46229260878519085 2311 80-81 0.24236842367506028 2311 82-83 0.44447562914059446 2311 84-85 0.4354977054397118 2311 86-87 0.5427782209264613 2311 88-89 0.7058869316989487 2311 90-91 1.8098810298863661 2311 92-93 2.978219385610412 2311 94-95 2.084888568506365 2311 96-97 1.475544332448873 2311 98-99 1.5602849967565042 2311 100-101 1.3535916283647307 2312 1 0.258398426934761 2312 2 0.14571721780362168 2312 3 0.05618033841901848 2312 4 -0.014809179966924546 2312 5 -0.025541489796516714 2312 6 -0.06110838777976113 2312 7 0.043104322756597924 2312 8 -0.7312498890381391 2312 9 -0.24423641097146032 2312 10-11 -0.15893741319109722 2312 12-13 -0.03215571461364419 2312 14-15 -0.21891393006893622 2312 16-17 0.018527221212899292 2312 18-19 0.040406327671377085 2312 20-21 0.041340078631733945 2312 22-23 -0.09074125929162591 2312 24-25 0.27413736639108066 2312 26-27 0.31280709223911174 2312 28-29 0.0725273298982998 2312 30-31 0.07873449946912814 2312 32-33 0.2231084565969681 2312 34-35 0.3081055410658493 2312 36-37 0.11701833796593775 2312 38-39 0.21102122730395223 2312 40-41 0.3932119723217724 2312 42-43 0.18456860131762198 2312 44-45 0.06671179293019946 2312 46-47 0.3525518688615037 2312 48-49 0.20271859317866614 2312 50-51 0.37156378906585985 2312 52-53 0.3863419135971995 2312 54-55 0.3452646849852776 2312 56-57 0.12077031642427727 2312 58-59 0.38045969413876435 2312 60-61 0.44366288048820124 2312 62-63 0.4552176218265025 2312 64-65 0.6100179498300946 2312 66-67 0.3271351032343297 2312 68-69 0.23359965742496414 2312 70-71 0.4981256729445107 2312 72-73 0.498626037943108 2312 74-75 0.7767238008841133 2312 76-77 0.8272112486642769 2312 78-79 0.9487515180486419 2312 80-81 0.6898001189995036 2312 82-83 0.9148809923036687 2312 84-85 1.0268325599922221 2312 86-87 1.15651704979134 2312 88-89 1.2857187626501592 2312 90-91 1.7070612760306254 2312 92-93 2.8959519748293054 2312 94-95 2.045111945388925 2312 96-97 1.6425615719146371 2312 98-99 1.4145273552775208 2312 100-101 1.4127867058868357 2313 1 -0.2851267308761116 2313 2 -1.230384394012276 2313 3 -0.5042045362515708 2313 4 -0.18489559291047897 2313 5 -1.067739810566266 2313 6 -0.5091411143839153 2313 7 -1.3041562030753582 2313 8 -0.9887665894119877 2313 9 -1.2535824917461085 2313 10-11 -2.1662963026928423 2313 12-13 -0.5618808479102029 2313 14-15 -0.008409548135979605 2313 16-17 -1.4768748134748506 2313 18-19 -1.0777235368870564 2313 20-21 -1.6889059837571878 2313 22-23 -1.3210057074132138 2313 24-25 -0.4332582945760137 2313 26-27 -0.8734602975997063 2313 28-29 -2.3532249529968325 2313 30-31 -0.5470422977362333 2313 32-33 -0.24414961780396283 2313 34-35 0.022742116416708313 2313 36-37 -0.007047053956604543 2313 38-39 -0.40763569492440155 2313 40-41 -0.2560480019382041 2313 42-43 -0.23706834174789293 2313 44-45 -0.7304537399496169 2313 46-47 -0.8130591503209388 2313 48-49 -0.40145496813775594 2313 50-51 -0.4045255655949198 2313 52-53 -0.1880023286041883 2313 54-55 -0.2533242100321864 2313 56-57 -0.6070856007173511 2313 58-59 -0.39102250234343217 2313 60-61 -0.4327803995265036 2313 62-63 -0.6184570732767618 2313 64-65 -0.6874524797831931 2313 66-67 -0.7000635239642996 2313 68-69 -0.6000676833852303 2313 70-71 -0.6314860816672407 2313 72-73 -0.3846260917375943 2313 74-75 -0.25305648961046145 2313 76-77 -0.22365134862688762 2313 78-79 -0.1959794373965984 2313 80-81 0.07701437407089884 2313 82-83 -0.1349734539793488 2313 84-85 0.06841649086186408 2313 86-87 0.24003577259577824 2313 88-89 0.44254140161565303 2313 90-91 1.1249092260214333 2313 92-93 2.1784487573260876 2313 94-95 1.380804781081764 2313 96-97 1.08410107059699 2313 98-99 1.0020587642375034 2313 100-101 1.0405935944080102 2314 1 -0.6193905028448015 2314 2 -0.6573031160129652 2314 3 -2.0417100745767804 2314 4 -0.70900084570426 2314 5 -0.4851916886738863 2314 6 -3.268676879657388 2314 7 -4.105734010390869 2314 8 -1.7023736390846054 2314 9 -2.0789135605198013 2314 10-11 -1.3320694528922203 2314 12-13 -2.4601639670949638 2314 14-15 -3.1392654039918284 2314 16-17 -2.341820907109472 2314 18-19 -2.962485758124693 2314 20-21 -2.589676426330911 2314 22-23 -3.5733859565147768 2314 24-25 -2.1945097099257893 2314 26-27 -2.0307228880590174 2314 28-29 -1.441406166997723 2314 30-31 -1.8318599022259718 2314 32-33 -2.3194745328829853 2314 34-35 -0.7886139117425941 2314 36-37 -0.6337307048205858 2314 38-39 -0.5511595466449748 2314 40-41 -1.0469909941107076 2314 42-43 -0.8950669140907266 2314 44-45 -1.4922023738294001 2314 46-47 -1.900365308938575 2314 48-49 -1.9981652029627455 2314 50-51 -0.9608355641180353 2314 52-53 -0.6750303402223636 2314 54-55 -0.3987606300587707 2314 56-57 -0.5423425790071192 2314 58-59 -0.4711731447891587 2314 60-61 -0.5467640553134423 2314 62-63 -0.4772347033658697 2314 64-65 -0.7650591848489121 2314 66-67 -0.6628336711606728 2314 68-69 -0.453310680480687 2314 70-71 -0.6005342281744035 2314 72-73 -0.46982030234164185 2314 74-75 -0.2971981722767367 2314 76-77 -0.3921653544359778 2314 78-79 -0.3686521100692701 2314 80-81 -0.5077724619454465 2314 82-83 -0.39631840548823405 2314 84-85 -0.4416509536318074 2314 86-87 -0.35438774928676153 2314 88-89 0.015414684734839312 2314 90-91 0.32537936829485403 2314 92-93 1.1088000102183244 2314 94-95 0.5870323200961849 2314 96-97 0.5444602002184169 2314 98-99 0.4592487410996071 2314 100-101 0.7237435476399323 2315 1 -7.524289718899034 2315 2 -3.372106502346764 2315 3 -1.7754297866299176 2315 4 -1.351240299646065 2315 5 -1.9830381475258427 2315 6 -2.2565968763347932 2315 7 -3.6746736027458518 2315 8 -3.3047421741172123 2315 9 -3.591904946355207 2315 10-11 -2.7050873389594514 2315 12-13 -3.1810488875179814 2315 14-15 -5.060759897554732 2315 16-17 -4.608543324703625 2315 18-19 -3.667708927116749 2315 20-21 -4.731416537586959 2315 22-23 -6.061332970460349 2315 24-25 -4.08713365667613 2315 26-27 -4.90126885301413 2315 28-29 -3.2387795164015643 2315 30-31 -2.9761105004200736 2315 32-33 -2.0247425187551755 2315 34-35 -2.7766972834298542 2315 36-37 -3.3879511199583874 2315 38-39 -2.487879701145605 2315 40-41 -3.8823890824323755 2315 42-43 -7.8996440631184015 2315 44-45 -4.453169731883854 2315 46-47 -2.935605044283772 2315 48-49 -5.54609924014359 2315 50-51 -6.701681795813041 2315 52-53 -6.9560041018991186 2315 54-55 -4.644201068742923 2315 56-57 -3.872480881992111 2315 58-59 -4.264331164658611 2315 60-61 -3.0512827199017885 2315 62-63 -3.334025806272205 2315 64-65 -2.0891437126633505 2315 66-67 -2.8366826931567566 2315 68-69 -2.0884873967235116 2315 70-71 -1.6409984674825608 2315 72-73 -2.3400150428105846 2315 74-75 -2.8700177621906278 2315 76-77 -3.028357602111644 2315 78-79 -1.9705922889638465 2315 80-81 -1.8097958540259746 2315 82-83 -2.136851448772653 2315 84-85 -2.0609978816306835 2315 86-87 -2.4215827677686903 2315 88-89 -1.9362663614754965 2315 90-91 -0.9804013311620885 2315 92-93 -0.36744185175670196 2315 94-95 -0.5566821707374388 2315 96-97 -0.9166191470613363 2315 98-99 -0.9990418943550132 2315 100-101 -0.9713594824473457 2316 1 -4.796734890760135 2316 2 -3.4187581125584074 2316 3 -5.151470768000561 2316 4 -4.728786035076784 2316 5 -4.536601775979957 2316 6 -4.8856607374552645 2316 7 -3.5565301287251465 2316 8 -1.9480318891945245 2316 9 -2.288471691290482 2316 10-11 -3.578752293166076 2316 12-13 -4.277979273922426 2316 14-15 -3.57844164920256 2316 16-17 -3.415724870428356 2316 18-19 -3.9294515580116354 2316 20-21 -6.345757231556711 2316 22-23 -7.081661190482489 2316 24-25 -4.808686740263077 2316 26-27 -4.808166987047777 2316 28-29 -5.48100402517246 2316 30-31 -7.07411462868788 2316 32-33 -5.357188087307954 2316 34-35 -6.8945510388074815 2316 36-37 -10.006249704883388 2316 38-39 -6.658345024944076 2316 40-41 -6.064601382905376 2316 42-43 -5.04959552410266 2316 44-45 -3.921976728455366 2316 46-47 -6.202658663627346 2316 48-49 -3.7737328494673577 2316 50-51 -4.962170279274119 2316 52-53 -5.908943096958751 2316 54-55 -7.789043118595924 2316 56-57 -5.377815382222998 2316 58-59 -6.321367071050069 2316 60-61 -6.901743327972195 2316 62-63 -7.103522310928209 2316 64-65 -5.531122874315653 2316 66-67 -7.120723407986251 2316 68-69 -5.845143793978583 2316 70-71 -6.79978757281356 2316 72-73 -8.870246626065025 2316 74-75 -8.377885629094774 2316 76-77 -7.79456234583099 2316 78-79 -6.776937550682298 2316 80-81 -6.740900092119428 2316 82-83 -5.861996597812837 2316 84-85 -7.009454483560834 2316 86-87 -5.705939944500628 2316 88-89 -5.691120708770594 2316 90-91 -6.367055152149842 2316 92-93 -4.8102809206277275 2316 94-95 -4.8491682954430395 2316 96-97 -5.352456607771032 2316 98-99 -6.302088486461473 2316 100-101 -5.526056245086657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11159.0 3 4626.0 4 4818.0 5 5391.0 6 6079.0 7 7301.0 8 8624.0 9 10731.0 10 12892.0 11 15156.0 12 17953.0 13 20020.0 14 22107.0 15 24739.0 16 27426.0 17 30279.0 18 28181.0 19 26712.0 20 28792.0 21 30914.0 22 34751.0 23 38114.0 24 43091.0 25 47962.0 26 54840.0 27 61850.0 28 69481.0 29 78994.0 30 89920.0 31 102269.0 32 118470.0 33 136041.0 34 160803.0 35 190212.0 36 218632.0 37 195032.0 38 62415.0 39 1095.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43960886122471 19.693635979762753 13.818734702814291 23.04802045619824 2 15.024996040908656 21.093256641009148 40.828105051800556 23.053642266281642 3 18.77678716747744 26.198772329665506 30.161990118226438 24.86245038463061 4 12.182668916986938 17.169787250889694 38.930100770749355 31.717443061374016 5 12.984213098729228 38.38841642228739 34.85083088954057 13.776539589442816 6 30.12487953820862 37.438938333336594 18.672701647663185 13.763480480791598 7 27.061433433754374 33.52715375757801 22.169768901279145 17.241643907388475 8 25.513260115652137 34.9214812700622 20.929938185927035 18.63532042835862 9 26.800971341135927 16.29122249898961 20.89287538185836 36.0149307780161 10-11 24.071990616294414 26.97688285030057 28.64972386491374 20.301402668491274 12-13 25.51353092279662 24.629886302323218 28.529422401726578 21.327160373153585 14-15 22.673925724665573 25.70609270646001 27.01147192161469 24.608509647259734 16-17 21.596230019816147 28.849126259097673 27.58252024563325 21.972123475452925 18-19 21.421664234689725 27.717841147388068 29.864611668552865 20.995882949369342 20-21 22.58252234034259 26.861520059810506 29.629075647627364 20.926881952219542 22-23 22.007641696718693 26.993149750760036 29.320098219658696 21.67911033286257 24-25 21.787551472398473 27.03015109653353 29.73513672227874 21.44716070878926 26-27 21.716986617070013 27.248811203549288 29.547383278635696 21.486818900745 28-29 21.525030304719518 27.322021201323576 29.681632968405342 21.47131552555156 30-31 21.777747786470368 27.208236330198428 29.549905454033347 21.464110429297854 32-33 21.570282768153977 27.219998210359087 29.466535426333053 21.743183595153887 34-35 21.955466827238194 27.224696721998814 29.469495444076344 21.350341006686648 36-37 22.071364167118148 26.90825361495387 29.37596204811854 21.64442016980944 38-39 21.695234472443726 27.3749774477558 29.43790273174295 21.491885348057522 40-41 21.94247805133716 27.15114496800289 29.44677684552356 21.459600135136387 42-43 21.66875025362395 27.2454214460012 29.23928460200719 21.846543698367665 44-45 21.778111404079763 27.325436297732303 29.123400693852542 21.773051604335393 46-47 22.023588328368778 27.428264921541746 28.552771318094784 21.995375431994695 48-49 21.907915778642625 27.513006149250636 28.57709450597173 22.00198356613501 50-51 21.944081698531246 27.639746844110558 28.5137851598609 21.902386297497298 52-53 21.999804345965245 27.779204421781184 28.318353571297557 21.902637660956014 54-55 21.918311068851636 27.668876484950932 28.32685746036218 22.08595498583525 56-57 21.904472244884886 27.92302936078899 28.094244193963934 22.078254200362185 58-59 21.95649405051337 27.569348745550993 28.33277228010808 22.14138492382755 60-61 21.951131864174833 27.77034210530819 28.32996100122206 21.94856502929492 62-63 21.83376768524451 27.635073014389228 28.486917740894196 22.04424155947207 64-65 21.85891880080541 27.574250625588743 28.461024806995056 22.105805766610793 66-67 21.752308473799 27.704863452069745 28.1188413605365 22.423986713594758 68-69 21.846064567257496 27.62083719047436 28.282770257965428 22.250327984302707 70-71 21.825632269977135 27.467382660329115 28.24889336099717 22.458091708696582 72-73 21.79052761180252 27.450656748498886 28.20635462095898 22.552461018739614 74-75 21.687398461568186 27.737554381067696 27.963430390399147 22.611616766964975 76-77 21.684822830052735 27.556492318257792 28.10572624735068 22.652958604338792 78-79 21.654462835788774 27.55338870301892 28.1278491909765 22.664299270215803 80-81 21.601499960133463 27.670189759827345 27.972221583596706 22.756088696442486 82-83 21.746467794859154 27.591800157704327 28.013820651440174 22.647911395996346 84-85 21.685741197367353 27.475075631386364 27.873430275833265 22.965752895413015 86-87 21.53269223574531 27.446596189457566 28.08661786421926 22.934093710577866 88-89 21.76367420181901 27.239934032870572 28.02106193577768 22.97532982953274 90-91 21.367120054229268 27.292852493305187 28.365785130967158 22.974242321498384 92-93 21.11576895158331 27.271392755943864 28.697506932959747 22.915331359513086 94-95 21.477153665773493 27.214710638535156 28.03460969928556 23.273525996405787 96-97 21.438500597934123 27.097337413651783 28.0394536234759 23.42470836493819 98-99 21.459659224631572 27.019436320765106 27.846616344163255 23.674288110440067 100-101 21.451900066817526 27.00277032166608 28.044937783365885 23.50039182815051 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1909.0 1 1146.5 2 400.0 3 416.0 4 390.0 5 390.0 6 571.5 7 981.0 8 1519.0 9 1784.5 10 1624.0 11 1463.5 12 1482.5 13 1619.0 14 1828.5 15 1974.5 16 2147.0 17 2391.0 18 2650.5 19 3017.0 20 3513.5 21 4237.5 22 5085.5 23 6093.0 24 7259.5 25 8955.5 26 11686.0 27 15015.5 28 18293.5 29 22052.0 30 26555.5 31 31615.5 32 37307.5 33 42571.0 34 47898.0 35 53105.5 36 58261.0 37 63172.5 38 65616.5 39 66828.0 40 67933.0 41 69228.5 42 70468.5 43 71916.0 44 73515.5 45 73901.5 46 74086.5 47 75740.5 48 79019.0 49 78191.5 50 73426.0 51 69372.5 52 65981.5 53 63243.0 54 60114.0 55 55671.0 56 50398.5 57 44936.5 58 40167.5 59 35502.0 60 30170.5 61 25093.5 62 20044.5 63 15285.5 64 11427.5 65 8403.0 66 6063.0 67 4395.5 68 3137.5 69 2202.0 70 1576.0 71 1079.5 72 699.0 73 494.5 74 351.0 75 244.5 76 170.5 77 102.0 78 66.5 79 47.0 80 28.5 81 18.0 82 17.5 83 15.0 84 12.0 85 11.0 86 6.0 87 2.5 88 4.0 89 4.5 90 3.0 91 2.0 92 2.0 93 2.0 94 1.0 95 0.5 96 0.5 97 0.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0851615725982874 2 0.09512313269579349 3 0.09194910619413714 4 0.09478131445715357 5 0.09141196324770298 6 0.07715325957872367 7 0.0888727420463779 8 0.08477092318269892 9 0.07954598724920307 10-11 0.08652884555284705 12-13 0.0851615725982874 14-15 0.0916317035439715 16-17 0.08855533939621227 18-19 0.09124105412838303 20-21 0.10144677011063191 22-23 0.11001664166510408 24-25 0.13294287924245266 26-27 0.1387537892993312 28-29 0.13726443840240016 30-31 0.13843638664916558 32-33 0.13521352897056066 34-35 0.1307454762797675 36-37 0.13457872367022938 38-39 0.12798651478217388 40-41 0.12359170885680355 42-43 0.12310339708731796 44-45 0.11409404494030877 46-47 0.13223482717669854 48-49 0.14991171323207703 50-51 0.16026392274517157 52-53 0.16832106694168383 54-55 0.1640727545471592 56-57 0.13577508750546907 58-59 0.12752261860116257 60-61 0.12459274798424902 62-63 0.12146755265954122 64-65 0.10737975810988186 66-67 0.12075950059378712 68-69 0.11834235733483343 70-71 0.12708313800862556 72-73 0.15330548003000188 74-75 0.16541561191324458 76-77 0.16988366460403775 78-79 0.16758859928745548 80-81 0.173399509344334 82-83 0.17286236639789987 84-85 0.15069301206325397 86-87 0.15125457059816239 88-89 0.17191015844740298 90-91 0.2652753687730483 92-93 0.5392671026314144 94-95 0.16963950871929495 96-97 0.1831413291455716 98-99 0.2012332802050128 100-101 0.207361592912057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2047872.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.141742447378256 #Duplication Level Percentage of deduplicated Percentage of total 1 79.61295913275485 50.269009610340575 2 11.801323192142608 14.903122190730809 3 3.858859601168993 7.309653572328161 4 1.692285110714431 4.2741532243305445 5 0.872513422700133 2.754600890900614 6 0.5295270699094589 2.0061157120282744 7 0.349878573613752 1.5464359948082924 8 0.23593068296824318 1.1917659535531893 9 0.16848557830240504 0.957462569214124 >10 0.80424653527855 8.976246830656462 >50 0.04868782977566958 2.112370808700157 >100 0.0242073195007566 2.5432699051291365 >500 7.828222644616897E-4 0.3364615816046774 >1k 2.348466793384876E-4 0.4646303609642682 >5k 7.828222644616254E-5 0.3547007947105945 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7176 0.3504125257828614 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4686 0.2288228951809488 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3472 0.16954184636539785 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.883117694855929E-5 2 0.0 0.0 0.0 0.0 4.883117694855929E-5 3 0.0 0.0 0.0 0.0 4.883117694855929E-5 4 0.0 0.0 0.0 0.0 4.883117694855929E-5 5 0.0 0.0 0.0 0.0 4.883117694855929E-5 6 0.0 0.0 0.0 0.0 4.883117694855929E-5 7 0.0 0.0 0.0 0.0 4.883117694855929E-5 8 0.0 0.0 0.0 0.0 4.883117694855929E-5 9 0.0 0.0 0.0 0.0 4.883117694855929E-5 10-11 0.0 0.0 0.0 2.4415588474279644E-5 4.883117694855929E-5 12-13 0.0 0.0 0.0 1.220779423713982E-4 1.9532470779423715E-4 14-15 0.0 2.4415588474279644E-5 0.0 2.1974029626851678E-4 2.685714732170761E-4 16-17 0.0 4.883117694855929E-5 0.0 3.1740265016563535E-4 2.929870616913557E-4 18-19 0.0 4.883117694855929E-5 0.0 4.3948059253703356E-4 2.929870616913557E-4 20-21 0.0 4.883117694855929E-5 0.0 4.883117694855928E-4 3.41818238639915E-4 22-23 0.0 4.883117694855929E-5 0.0 0.0012451950121882618 3.41818238639915E-4 24-25 0.0 4.883117694855929E-5 0.0 0.002124156197262329 3.41818238639915E-4 26-27 0.0 4.883117694855929E-5 0.0 0.0027833770860678795 3.662338271141946E-4 28-29 0.0 4.883117694855929E-5 0.0 0.005005195637227327 3.906494155884743E-4 30-31 0.0 4.883117694855929E-5 0.0 0.011963638352397025 3.906494155884743E-4 32-33 0.0 4.883117694855929E-5 0.0 0.027150134383398965 4.3948059253703356E-4 34-35 0.0 4.883117694855929E-5 0.0 0.04873351459466217 4.883117694855928E-4 36-37 0.0 4.883117694855929E-5 0.0 0.08425819582473905 4.883117694855928E-4 38-39 0.0 4.883117694855929E-5 0.0 0.1327719701231327 5.127273579598725E-4 40-41 0.0 4.883117694855929E-5 0.0 0.18966029126820427 5.371429464341522E-4 42-43 0.0 4.883117694855929E-5 0.0 0.24899017126070377 5.371429464341522E-4 44-45 0.0 4.883117694855929E-5 0.0 0.31715849428089254 5.371429464341522E-4 46-47 0.0 4.883117694855929E-5 0.0 0.4002691574473405 5.615585349084318E-4 48-49 0.0 4.883117694855929E-5 0.0 0.49216943246452904 5.859741233827114E-4 50-51 0.0 4.883117694855929E-5 0.0 0.5985969826239139 7.324676542283892E-4 52-53 0.0 4.883117694855929E-5 0.0 0.7001169994999688 7.812988311769486E-4 54-55 0.0 4.883117694855929E-5 0.0 0.8101824723420215 8.545455965997875E-4 56-57 0.0 4.883117694855929E-5 0.0 0.9433450918807426 9.766235389711856E-4 58-59 0.0 4.883117694855929E-5 0.0 1.0921092724545285 0.001025454715919745 60-61 0.0 4.883117694855929E-5 0.0 1.2496386492905807 0.001025454715919745 62-63 0.0 4.883117694855929E-5 0.0 1.4121243905869116 0.0010742858928683044 64-65 0.0 4.883117694855929E-5 0.0 1.5842787049190574 0.0011231170698168635 66-67 0.0 4.883117694855929E-5 0.0 1.7742563988374274 0.0011231170698168635 68-69 0.0 4.883117694855929E-5 0.0 1.994069941871367 0.001171948246765423 70-71 0.0 4.883117694855929E-5 0.0 2.2376642680792553 0.0011963638352397025 72-73 0.0 4.883117694855929E-5 0.0 2.4949069082442654 0.0012696106006625414 74-75 0.0 4.883117694855929E-5 0.0 2.7585952637664852 0.0013184417776111008 76-77 0.0 4.883117694855929E-5 0.0 3.045966740108757 0.0013184417776111008 78-79 0.0 4.883117694855929E-5 0.0 3.373599521845115 0.00136727295455966 80-81 0.0 4.883117694855929E-5 0.0 3.7297741265079067 0.00136727295455966 82-83 0.0 4.883117694855929E-5 0.0 4.083409509969373 0.00136727295455966 84-85 0.0 4.883117694855929E-5 0.0 4.434261516344772 0.00136727295455966 86-87 0.0 4.883117694855929E-5 0.0 4.812751968873055 0.0013916885430339397 88-89 0.0 4.883117694855929E-5 0.0 5.22571723232702 0.0014161041315082193 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9445 0.0 32.812824 1 GGTATCA 6065 0.0 32.08377 1 TACATGG 8380 0.0 27.694738 2 GTACATG 8575 0.0 27.56307 1 TATCAAC 11500 0.0 26.949316 2 ATCAACG 11360 0.0 26.94721 3 TCAACGC 11300 0.0 26.922289 4 CAACGCA 11390 0.0 26.792894 5 AACGCAG 11760 0.0 26.147875 6 ACATGGG 8525 0.0 25.219482 3 ACGCAGA 13405 0.0 22.691328 7 CGCAGAG 13400 0.0 22.52328 8 GAGTACT 6105 0.0 20.948988 12-13 CATGGGA 5745 0.0 20.570353 4 GCAGAGT 13725 0.0 20.019634 9 AGTACTT 6480 0.0 19.956366 12-13 CATGGGG 3305 0.0 19.673494 4 GTACTTT 6760 0.0 19.166042 14-15 AGAGTAC 10915 0.0 18.956493 10-11 AGTACAT 5990 0.0 18.302801 2 >>END_MODULE