FastQCFastQC Report
Fri 27 May 2016
SRR939613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939613_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047872
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77100.3764883742733921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55250.26979225264079004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40390.19722912369523093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57050.034.727931
GTATCAA90250.033.997951
ATCAACG112300.027.1030183
TCAACGC112200.027.0852434
CAACGCA113400.026.7571435
TATCAAC115200.026.4628662
AACGCAG115600.026.2958876
TACATGG83050.023.621682
GTACATG84350.023.5997641
ACGCAGA132150.022.6827077
CGCAGAG132100.022.4428778
ACATGGG83250.022.0945723
GCAGAGT142450.020.2845139
GAGTACT66850.020.24151612-13
AGTACTT68550.020.0822412-13
AGAGTAC116550.019.14843610-11
CATGGGG32500.018.9618664
GTACTTT71950.018.54531314-15
CATGGGA54400.018.0734254
GTATAGG6450.017.5107171
Produced by FastQC (version 0.11.5)