##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939613_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2047872 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.530316347896743 31.0 31.0 34.0 30.0 34.0 2 31.735320371585725 31.0 31.0 34.0 30.0 34.0 3 31.778361147571722 31.0 31.0 34.0 30.0 34.0 4 35.575228334583414 37.0 35.0 37.0 33.0 37.0 5 35.31016733467717 37.0 35.0 37.0 33.0 37.0 6 35.33244753578349 37.0 35.0 37.0 32.0 37.0 7 35.260940136883555 37.0 35.0 37.0 32.0 37.0 8 35.21889991171323 37.0 35.0 37.0 32.0 37.0 9 36.82597886977311 39.0 37.0 39.0 32.0 39.0 10-11 36.70582438746172 39.0 35.0 39.0 32.0 39.0 12-13 36.65391293987125 39.0 35.0 39.0 32.0 39.0 14-15 37.71022480897244 39.5 37.0 41.0 32.0 41.0 16-17 37.67457707317645 39.0 36.0 41.0 32.0 41.0 18-19 37.662164676307896 39.0 36.5 41.0 32.0 41.0 20-21 37.60597219943433 39.0 36.0 41.0 32.0 41.0 22-23 37.47005721060691 39.0 36.0 41.0 32.0 41.0 24-25 37.35391347701419 39.0 36.0 41.0 31.5 41.0 26-27 37.09712252523595 39.0 36.0 40.0 31.0 41.0 28-29 36.901940160322525 39.0 36.0 40.0 30.5 41.0 30-31 36.65506559980311 38.5 35.5 40.0 30.0 41.0 32-33 36.483217945262204 38.0 35.0 40.0 30.0 41.0 34-35 36.46898438964935 38.0 35.0 40.0 30.0 41.0 36-37 36.36460018008938 38.0 35.0 40.0 30.0 41.0 38-39 36.39390792002625 38.5 35.0 40.0 30.0 41.0 40-41 36.297869935230324 38.5 35.0 40.0 30.0 41.0 42-43 36.10636016313519 38.0 35.0 40.0 29.5 41.0 44-45 35.8902441168198 38.0 35.0 40.0 29.0 41.0 46-47 35.58802869515282 38.0 34.5 40.0 27.5 41.0 48-49 35.294178786564785 38.0 34.0 40.0 27.0 41.0 50-51 35.051021987702356 38.0 34.0 40.0 26.0 41.0 52-53 34.82404076036002 38.0 34.0 40.0 26.0 41.0 54-55 34.54706739483718 38.0 33.5 40.0 25.5 41.0 56-57 32.502213273095194 37.0 30.5 39.5 16.0 41.0 58-59 27.46642465935371 32.5 17.5 38.5 6.5 40.0 60-61 28.245476768079257 33.0 20.0 38.0 9.0 40.0 62-63 31.357946199762484 35.0 29.5 38.5 12.5 40.0 64-65 31.60078266610413 35.5 30.5 39.0 5.0 40.0 66-67 24.877568519907495 27.0 18.0 35.5 2.0 39.0 68-69 17.347126187574226 13.0 7.5 28.0 2.0 35.5 70-71 16.225768993374587 12.5 7.5 26.5 2.0 33.5 72-73 17.330233286064754 13.5 7.0 28.5 2.0 34.0 74-75 18.366243593349587 17.5 8.5 29.5 2.0 34.5 76-77 19.58189183698981 21.5 8.0 30.0 2.0 34.5 78-79 22.69410221927933 27.5 10.5 33.5 2.0 36.0 80-81 26.353019866476032 31.5 22.5 34.5 2.0 36.0 82-83 27.66972008016126 33.5 26.5 35.0 2.0 36.0 84-85 27.711916076786046 34.0 27.0 35.0 2.0 36.0 86-87 27.461667037783613 34.0 27.0 35.0 2.0 36.0 88-89 27.04058212622664 33.0 26.0 35.0 2.0 35.5 90-91 25.69060786025689 32.5 20.0 35.0 2.0 35.0 92-93 23.190682083645854 31.5 2.0 34.5 2.0 35.0 94-95 15.783795080942557 4.5 2.0 32.0 2.0 35.0 96-97 13.21038204536221 2.0 2.0 30.0 2.0 34.0 98-99 12.864941998328021 2.0 2.0 29.5 2.0 34.0 100-101 12.57630213216451 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.42001625413264776 1101 2 0.4041968630065256 1101 3 0.4597571760238317 1101 4 0.28379618632897063 1101 5 0.47227636524926453 1101 6 0.40460649244516134 1101 7 0.40203336544482937 1101 8 0.4298449307372394 1101 9 0.48312018697482273 1101 10-11 0.5078302554288427 1101 12-13 0.5352610307022232 1101 14-15 0.6529582141265138 1101 16-17 0.7132305545603046 1101 18-19 0.555822277132016 1101 20-21 0.5870790670646713 1101 22-23 0.5614743861331633 1101 24-25 0.5474917937119557 1101 26-27 0.5154694303459237 1101 28-29 0.5368015446355798 1101 30-31 0.6622221658511691 1101 32-33 0.8034071861619267 1101 34-35 0.7148614954697692 1101 36-37 0.5283450107518703 1101 38-39 0.5999163315877922 1101 40-41 0.6227628455209029 1101 42-43 0.6817950126175774 1101 44-45 0.5887060693445108 1101 46-47 0.5674043105646973 1101 48-49 0.3871914029201591 1101 50-51 0.21799224646530035 1101 52-53 0.18086927831652133 1101 54-55 0.29242474614407854 1101 56-57 -1.959423332749651 1101 58-59 -9.288540133648546 1101 60-61 -9.718988582752637 1101 62-63 -3.6765045328157626 1101 64-65 -4.90157792138012 1101 66-67 -9.956579221481444 1101 68-69 -4.833078949902935 1101 70-71 -4.701822585150492 1101 72-73 -4.7228623253166795 1101 74-75 -6.52536196472143 1101 76-77 -7.778534461247066 1101 78-79 -11.262184570307081 1101 80-81 -8.00299283664733 1101 82-83 -4.261419241942146 1101 84-85 -3.5633520744704015 1101 86-87 -3.2846200194149695 1101 88-89 -3.2780971162580776 1101 90-91 -9.693878603547944 1101 92-93 -17.039027514846595 1101 94-95 -11.298725165755092 1101 96-97 -9.086396303155894 1101 98-99 -9.146965050132367 1101 100-101 -9.120029589034266 1102 1 0.4212050708253443 1102 2 0.361436015712826 1102 3 0.43624989015820503 1102 4 0.2998705687123575 1102 5 0.26984195845727044 1102 6 0.35392634769272746 1102 7 0.38191634768205773 1102 8 0.3996328504496347 1102 9 0.5527509260203374 1102 10-11 0.4470142134507711 1102 12-13 0.35455282526547194 1102 14-15 0.4614490656716299 1102 16-17 0.4452389994398871 1102 18-19 0.46465600843902166 1102 20-21 0.5557634619190992 1102 22-23 0.6447551118580037 1102 24-25 0.5297005426783272 1102 26-27 0.4171905141910841 1102 28-29 0.5328239483965831 1102 30-31 0.546063413706932 1102 32-33 0.4904256701209704 1102 34-35 0.4502450600954617 1102 36-37 0.466311831240219 1102 38-39 0.32036855614045123 1102 40-41 0.40250952547860663 1102 42-43 0.49132319728789753 1102 44-45 0.6528325212404056 1102 46-47 0.6573748470108143 1102 48-49 0.5886658649312082 1102 50-51 0.6134419106163591 1102 52-53 0.5002513043960661 1102 54-55 0.2848163193107993 1102 56-57 -2.616523482817179 1102 58-59 -10.01240567815129 1102 60-61 -10.353017785491328 1102 62-63 -4.16010281420176 1102 64-65 -5.27557751237449 1102 66-67 -6.675184334447021 1102 68-69 -4.481122029195033 1102 70-71 -4.313030953102761 1102 72-73 -4.518227784362933 1102 74-75 -5.431864660305649 1102 76-77 -7.3787704792314575 1102 78-79 -10.114209823276408 1102 80-81 -5.840610520443825 1102 82-83 -3.1895674574163273 1102 84-85 -2.712407129855759 1102 86-87 -2.6950961948368963 1102 88-89 -2.7291022261939837 1102 90-91 -8.647490877405207 1102 92-93 -12.760885572767986 1102 94-95 -10.465073552273978 1102 96-97 -9.1595016142027 1102 98-99 -9.282558156049348 1102 100-101 -9.17114821285216 1103 1 0.26069524955432044 1103 2 0.15962673326274057 1103 3 0.2180982479946323 1103 4 0.1939233358276553 1103 5 0.27617457489706254 1103 6 0.21123341936588957 1103 7 0.1843736473375941 1103 8 0.1526204041207535 1103 9 0.39235260257945015 1103 10-11 0.3780292784442949 1103 12-13 0.2999690113747917 1103 14-15 0.4349279258364689 1103 16-17 0.37761646051153974 1103 18-19 0.36947279141974576 1103 20-21 0.25334656744054485 1103 22-23 0.40619987823680503 1103 24-25 0.5240357624390128 1103 26-27 0.4105016672985826 1103 28-29 0.3284149252556716 1103 30-31 0.3547036004913764 1103 32-33 0.527902111089773 1103 34-35 0.4211906691012146 1103 36-37 0.31262127863549694 1103 38-39 0.38525198209029554 1103 40-41 0.5534053440542479 1103 42-43 0.5709656370445515 1103 44-45 0.5128541559111142 1103 46-47 0.5984717708470555 1103 48-49 0.5601496555505534 1103 50-51 0.6258356871961865 1103 52-53 0.686009502427467 1103 54-55 0.5049499888424904 1103 56-57 -2.1922174673046584 1103 58-59 -9.75659960765704 1103 60-61 -10.283453496507367 1103 62-63 -3.9574639469787947 1103 64-65 -4.948589922901316 1103 66-67 -6.0847823540818915 1103 68-69 -3.9563216895869644 1103 70-71 -3.8975126799893367 1103 72-73 -4.083610776852172 1103 74-75 -4.8980025647090475 1103 76-77 -6.543671678344591 1103 78-79 -8.966025882544152 1103 80-81 -4.525424567124588 1103 82-83 -2.2369411209128067 1103 84-85 -1.8555622882926421 1103 86-87 -1.6644566678331927 1103 88-89 -1.7265457036987861 1103 90-91 -5.027813044759359 1103 92-93 -10.453579396146218 1103 94-95 -8.519671814851367 1103 96-97 -8.343886419810325 1103 98-99 -8.519234932534559 1103 100-101 -8.620729456666714 1104 1 0.2498945971949169 1104 2 0.2507939597632216 1104 3 0.16910432876191095 1104 4 0.16656852359699315 1104 5 0.20477686247523508 1104 6 0.2164339334769423 1104 7 0.3318770765447212 1104 8 0.1856887614495335 1104 9 0.18408362313309112 1104 10-11 0.27108337891196044 1104 12-13 0.2911297574838585 1104 14-15 0.2846962518433571 1104 16-17 0.3563310748742552 1104 18-19 0.23855970426928508 1104 20-21 0.26450904165399436 1104 22-23 0.3164885796743775 1104 24-25 0.3042343864359083 1104 26-27 0.3662870367348958 1104 28-29 0.4311842125206198 1104 30-31 0.4310906742706351 1104 32-33 0.3296426362174927 1104 34-35 0.38529470098473695 1104 36-37 0.2785635815505927 1104 38-39 0.2630561014590995 1104 40-41 0.20101299955663876 1104 42-43 0.27926701844798885 1104 44-45 0.3703060660064921 1104 46-47 0.13793195424213422 1104 48-49 0.152340243862092 1104 50-51 0.23147200762993947 1104 52-53 0.09692140150616524 1104 54-55 -0.06886436954476238 1104 56-57 -2.959054064235115 1104 58-59 -10.40759758793505 1104 60-61 -10.76954387636631 1104 62-63 -4.551835727034312 1104 64-65 -5.599076492290106 1104 66-67 -6.661971376298833 1104 68-69 -4.066358735824556 1104 70-71 -4.151436556991506 1104 72-73 -4.399590342017909 1104 74-75 -5.13956023479232 1104 76-77 -6.812577680267106 1104 78-79 -9.408733822103702 1104 80-81 -5.266773784617328 1104 82-83 -3.0501811006075776 1104 84-85 -2.6206486395082536 1104 86-87 -2.5605257704069864 1104 88-89 -2.420884001731988 1104 90-91 -5.333810647014534 1104 92-93 -7.504652440958122 1104 94-95 -8.15603383111715 1104 96-97 -7.952204542224919 1104 98-99 -8.429349203436521 1104 100-101 -8.338147668726723 1105 1 0.27889866294740173 1105 2 0.29743814858139217 1105 3 0.29997019289085003 1105 4 0.21386178947555834 1105 5 0.1865407262030132 1105 6 0.19230049274236904 1105 7 0.28279449751406815 1105 8 0.21489446984667637 1105 9 0.2725644156099136 1105 10-11 0.44515574315074247 1105 12-13 0.30925562706776333 1105 14-15 0.4879331469286967 1105 16-17 0.438446009059156 1105 18-19 0.43406148137774636 1105 20-21 0.3699890519696041 1105 22-23 0.4847958745746226 1105 24-25 0.5429024412529913 1105 26-27 0.5567523112182826 1105 28-29 0.528841655628213 1105 30-31 0.5873403757864537 1105 32-33 0.6567282313975937 1105 34-35 0.7030830844581075 1105 36-37 0.47799335737703785 1105 38-39 0.44716241595158124 1105 40-41 0.5442193935387678 1105 42-43 0.43897255651015854 1105 44-45 0.4529443159181312 1105 46-47 0.5202167988870912 1105 48-49 0.5466451126829313 1105 50-51 0.46855472674916143 1105 52-53 0.5714999949107522 1105 54-55 0.5225636955030311 1105 56-57 1.631893136169019 1105 58-59 -8.239193080054537 1105 60-61 -9.555589037951052 1105 62-63 -3.306077916830983 1105 64-65 -4.63036378954639 1105 66-67 -6.137572297911845 1105 68-69 -3.8728636214135825 1105 70-71 -3.7972995397531832 1105 72-73 -2.0113666969768857 1105 74-75 -4.2622728377759955 1105 76-77 -6.360842120798974 1105 78-79 -8.904331963170774 1105 80-81 -4.3801787753789085 1105 82-83 -1.9102221083538389 1105 84-85 -1.4019869311280928 1105 86-87 -1.3620432904996171 1105 88-89 -1.3511869644992025 1105 90-91 -3.813199160388219 1105 92-93 -6.072818479879398 1105 94-95 -7.616452441247464 1105 96-97 -7.8416041883951415 1105 98-99 -8.307334284003371 1105 100-101 -8.385927424017455 1106 1 0.22371131329880356 1106 2 0.20115528126494553 1106 3 0.16807597402724284 1106 4 0.1791225854959535 1106 5 -9.908761327181992E-4 1106 6 0.21648164768248535 1106 7 0.177013124513401 1106 8 -0.011093256202450164 1106 9 0.1778001166842884 1106 10-11 0.23533646414657028 1106 12-13 0.26480094526083775 1106 14-15 0.35849956252759796 1106 16-17 0.386116326867743 1106 18-19 0.3818076329615394 1106 20-21 0.34056684855005415 1106 22-23 0.3669394221141147 1106 24-25 0.446588696221518 1106 26-27 0.4272016044498699 1106 28-29 0.2937886583485252 1106 30-31 0.4334139016594918 1106 32-33 0.25505624453612086 1106 34-35 0.5217120003379776 1106 36-37 0.48147721543040234 1106 38-39 0.43876332254968986 1106 40-41 0.3711186865219105 1106 42-43 0.18742092175401126 1106 44-45 0.18782399575527364 1106 46-47 0.35542136907284316 1106 48-49 0.3908136846584185 1106 50-51 0.4746131473859805 1106 52-53 0.4028630697538418 1106 54-55 0.20393489792858333 1106 56-57 1.4979241951279434 1106 58-59 -8.835190712999665 1106 60-61 -9.981188414623446 1106 62-63 -3.7987947984704284 1106 64-65 -5.56099351274246 1106 66-67 -6.91068758015664 1106 68-69 -4.283551072923146 1106 70-71 -4.362954198182953 1106 72-73 -4.5871310268985255 1106 74-75 -5.018478962731599 1106 76-77 -6.460128407851936 1106 78-79 -9.182738100477465 1106 80-81 -4.871445232550336 1106 82-83 -2.551416671320599 1106 84-85 -2.1499452753159467 1106 86-87 -2.0232078086215246 1106 88-89 -1.9829007675949732 1106 90-91 -4.395531925618915 1106 92-93 -6.694142819161364 1106 94-95 -7.4436806233024715 1106 96-97 -7.299734480048765 1106 98-99 -6.589378786243957 1106 100-101 -7.062372625301116 1107 1 0.07060278687799482 1107 2 -0.02213700667325824 1107 3 -0.05096946796923163 1107 4 0.014165535810832353 1107 5 -0.004182528440708211 1107 6 -0.010526514064522985 1107 7 0.10554976903954127 1107 8 0.15245663585726987 1107 9 0.11383368734502142 1107 10-11 0.09714590357873476 1107 12-13 0.09201828349124952 1107 14-15 0.17603265342788177 1107 16-17 0.12640612452103284 1107 18-19 0.0377650425251872 1107 20-21 0.1598782654518658 1107 22-23 0.11507810989365197 1107 24-25 0.18407674876657865 1107 26-27 0.12057253026880232 1107 28-29 0.18090575201983228 1107 30-31 0.12582926386349413 1107 32-33 0.08158047907263466 1107 34-35 0.1477968152798752 1107 36-37 0.19735940432984478 1107 38-39 0.282059386186269 1107 40-41 0.2627613806852196 1107 42-43 0.13125205445886934 1107 44-45 0.20107036542826506 1107 46-47 0.2089828163525027 1107 48-49 0.028795557581723585 1107 50-51 3.495831546516115E-4 1107 52-53 -0.09991749233637393 1107 54-55 -0.1236417368766567 1107 56-57 1.1732548713504016 1107 58-59 -8.466962819108428 1107 60-61 -10.159987053509788 1107 62-63 -4.000743915464472 1107 64-65 -5.811543107308804 1107 66-67 -6.828231402234188 1107 68-69 -3.7171587224601232 1107 70-71 -3.93884026520416 1107 72-73 -2.3202191803771726 1107 74-75 -4.738963220490287 1107 76-77 -6.701524145270039 1107 78-79 -7.43177264369478 1107 80-81 -2.5677774744538837 1107 82-83 -1.3507802031023424 1107 84-85 -1.112996039603896 1107 86-87 -1.090334930473226 1107 88-89 -1.002873443829536 1107 90-91 -0.6602626196257972 1107 92-93 -3.5698037409625094 1107 94-95 -6.090469277771133 1107 96-97 -6.72356477008851 1107 98-99 -6.353481089513078 1107 100-101 -6.921357972704939 1108 1 -0.14337479200611725 1108 2 -0.11575132701735669 1108 3 -0.127070351174158 1108 4 -0.06785503179013119 1108 5 -0.04028850965572417 1108 6 -0.2262120107322474 1108 7 -0.0884020834625332 1108 8 -0.16413823102052305 1108 9 -0.22100218036330688 1108 10-11 -0.09021082039271988 1108 12-13 -0.21767169166017197 1108 14-15 -0.040890840971059106 1108 16-17 -0.13690546349223354 1108 18-19 -0.14077609098865906 1108 20-21 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13.431940588934104 2316 98-99 13.213971221565428 2316 100-101 12.007955074672083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 26.0 5 68.0 6 183.0 7 417.0 8 973.0 9 1737.0 10 3022.0 11 4659.0 12 6931.0 13 9859.0 14 12369.0 15 16533.0 16 23092.0 17 30987.0 18 33676.0 19 34088.0 20 35000.0 21 35319.0 22 35241.0 23 29995.0 24 30383.0 25 36673.0 26 50843.0 27 76203.0 28 108393.0 29 145521.0 30 179840.0 31 197622.0 32 198195.0 33 181594.0 34 160453.0 35 143583.0 36 117290.0 37 75376.0 38 29687.0 39 2026.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.75305693332258 20.51279797010347 14.635915394928677 23.098229701645263 2 15.339266747243894 21.79120731543542 39.85701204995788 23.012513887362804 3 18.46327760863143 26.409504532769496 30.816679810436725 24.310538048162357 4 12.508594287142946 17.947899087442963 38.52794510594412 31.01556151946997 5 12.714642037933766 38.68522232018037 35.107826497696195 13.49230914418967 6 30.137723451465714 38.09486139758735 18.23556355084693 13.531851600100007 7 26.798562995423055 33.628680712798804 22.18303789643 17.389718395348144 8 25.226288093945204 35.804548419987576 20.60530356005766 18.363859926009553 9 26.131809019313707 16.51939183698981 20.88465490030627 36.46414424339021 10-11 23.963143204262767 27.259418557409838 28.64602865804113 20.13140958028627 12-13 25.242495624726548 24.754379179948746 28.690758016126004 21.3123671791987 14-15 22.327082337883855 25.92080857070098 27.062487898956007 24.689621192459153 16-17 21.432320965372835 29.068247429526846 27.61317601881367 21.886255586286644 18-19 21.193396647930115 27.928055944984763 29.87998744060289 20.99855996648223 20-21 22.410897751421963 26.991286564785298 29.617231936371024 20.980583747421715 22-23 21.80157607814566 27.094788335966967 29.446868645830687 21.65676694005668 24-25 21.688250459074215 27.18461583160858 29.75215974244478 21.37497396687243 26-27 21.666500233291575 27.362550244364076 29.54443075268822 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22.02235203154523 27.714021655088416 28.359011728780626 21.90461458458573 54-55 21.899758712317887 27.61843560062307 28.381955346507436 22.0998503405516 56-57 21.099866710801727 27.721010090493248 29.22002245387673 21.95910074482829 58-59 20.713179171731895 27.205530025729995 30.211242039868214 21.870048762669896 60-61 21.846097805657717 27.60685688701728 28.77829207185019 21.76875323547482 62-63 21.91824671801681 27.58775273211702 28.542058657926106 21.951941891940066 64-65 21.483970216555534 27.55393965232114 28.834542693979483 22.127547437143843 66-67 21.534277403189687 27.260384291281685 29.21024494617258 21.995093359356037 68-69 21.760449240975387 26.88668510003181 29.616910412085044 21.73595524690776 70-71 21.722098114466544 26.880483728820032 29.489836730469104 21.90758142624432 72-73 20.88685995704324 27.05638815011487 29.942649060515254 22.114102832326644 74-75 21.375783776993856 27.25806781244283 29.176068999791145 22.19007941077217 76-77 19.552611623516366 27.788299488657238 29.964571906197925 22.694516981628475 78-79 20.34943582727872 27.734324256037663 29.307130576251133 22.609109340432482 80-81 21.311233916412863 27.805987275555346 28.217407566019386 22.665371242012412 82-83 21.763267945004266 27.74732062808736 27.933611932019055 22.55579949488932 84-85 21.750346762987956 27.632462728930108 27.78774613702003 22.82944437106191 86-87 21.55219329803964 27.61890624057707 28.00362426499618 22.82527619638711 88-89 21.74471206151959 27.476233819757912 27.887686120394477 22.891367998328015 90-91 21.02347911523374 27.64265492539564 28.398342593095737 22.935523366274882 92-93 20.045390952746683 27.707210214092033 29.126939981521012 23.120458851640276 94-95 19.419809589038024 27.7305185669256 29.474793439821706 23.374878404214673 96-97 19.449232323464933 27.817079269754863 29.029807446329727 23.70388096045048 98-99 19.689456798400627 27.676000207131214 28.818715345474295 23.815827648993864 100-101 20.047412386190057 27.721848649171022 28.573851595681216 23.656887368957708 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 344.0 1 268.5 2 199.5 3 209.0 4 233.5 5 305.5 6 391.5 7 538.0 8 784.5 9 942.5 10 1043.0 11 1194.5 12 1355.5 13 1554.0 14 1770.0 15 1999.0 16 2287.5 17 2579.5 18 2887.0 19 3272.0 20 3754.5 21 4385.0 22 5190.5 23 6291.0 24 7735.0 25 9633.0 26 12284.5 27 15479.5 28 18898.5 29 23063.0 30 28000.5 31 33193.5 32 38888.0 33 45100.0 34 51010.0 35 56528.5 36 61737.5 37 65969.0 38 68847.5 39 70847.5 40 72419.5 41 73987.0 42 75992.0 43 77669.0 44 78685.5 45 78924.5 46 79239.0 47 79797.0 48 79663.5 49 78135.5 50 74219.5 51 70044.0 52 66172.0 53 61901.0 54 57126.5 55 51734.0 56 46056.5 57 40144.0 58 34427.0 59 29016.0 60 23774.0 61 18671.0 62 14300.0 63 10633.5 64 7613.0 65 5394.0 66 3796.0 67 2624.5 68 1738.5 69 1130.5 70 749.0 71 460.5 72 305.0 73 207.0 74 127.0 75 81.0 76 48.5 77 23.5 78 9.5 79 3.0 80 2.0 81 1.5 82 1.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023292471404462778 2 0.007178183011438215 3 3.41818238639915E-4 4 0.0 5 0.13579950309394337 6 0.0 7 4.883117694855929E-5 8 0.0019532470779423713 9 0.0 10-11 0.0 12-13 0.0 14-15 5.615585349084318E-4 16-17 0.0 18-19 1.4649353084567786E-4 20-21 0.0 22-23 1.9532470779423715E-4 24-25 2.4415588474279644E-5 26-27 2.6857147321707604E-4 28-29 0.0029787017938621165 30-31 0.011719482467654228 32-33 0.002661299143696481 34-35 0.0038576629789361834 36-37 0.1078436542908932 38-39 0.10711118663666479 40-41 0.11929456528533032 42-43 0.1217361241327583 44-45 0.11731690261891367 46-47 0.11060261578848679 48-49 0.1103340443152697 50-51 0.10952832989561848 52-53 0.08760313144571535 54-55 0.0761522204512782 56-57 0.5712271079442466 58-59 1.6069607866116633 60-61 0.531454114319645 62-63 0.004931948871804488 64-65 0.1224441761985124 66-67 1.9224590208763048 68-69 2.911778665854116 70-71 2.81589864991562 72-73 2.671309534970936 74-75 2.037285533470842 76-77 1.4433763438339897 78-79 0.9502302878304895 80-81 0.0576940355647228 82-83 1.9532470779423715E-4 84-85 1.709091193199575E-4 86-87 4.15065004062754E-4 88-89 1.9532470779423715E-4 90-91 0.25211536658541156 92-93 0.4032722748296768 94-95 2.038091247890493 96-97 2.4586985905369083 98-99 1.4565119304331522 100-101 0.9624624976561035 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2047872.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.65308890472279 #Duplication Level Percentage of deduplicated Percentage of total 1 75.83167393133748 43.719302389573485 2 13.538184018953247 15.610362537064178 3 4.646111147867421 8.035879772076724 4 2.082431488692755 4.802344310223906 5 1.1367323907514812 3.2768066792436614 6 0.6664428361284411 2.3053492848737167 7 0.43312313237597583 1.7479620520294905 8 0.30359756942746785 1.4002670129167645 9 0.20911739409635124 1.0850637342024803 >10 1.0484002427661778 10.628929302796816 >50 0.0702759389191909 2.7601942022273094 >100 0.03263582891668961 3.303837246533694 >500 7.644478604229853E-4 0.30199648566307385 >1k 3.3975460463243277E-4 0.3735902690325222 >5k 1.6987730231621639E-4 0.6481147215421984 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7710 0.3764883742733921 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5525 0.26979225264079004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4039 0.19722912369523093 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.883117694855929E-5 0.0 5 0.0 0.0 0.0 4.883117694855929E-5 0.0 6 0.0 0.0 0.0 4.883117694855929E-5 4.883117694855929E-5 7 0.0 0.0 0.0 4.883117694855929E-5 4.883117694855929E-5 8 0.0 0.0 0.0 4.883117694855929E-5 4.883117694855929E-5 9 0.0 0.0 0.0 9.766235389711857E-5 4.883117694855929E-5 10-11 0.0 0.0 0.0 1.220779423713982E-4 4.883117694855929E-5 12-13 0.0 0.0 0.0 2.1974029626851678E-4 3.4181823863991504E-4 14-15 0.0 0.0 0.0 3.1740265016563535E-4 4.3948059253703356E-4 16-17 0.0 0.0 0.0 4.1506500406275393E-4 4.883117694855928E-4 18-19 0.0 0.0 0.0 4.638961810113132E-4 4.883117694855928E-4 20-21 0.0 0.0 0.0 5.371429464341522E-4 6.103897118569911E-4 22-23 0.0 0.0 0.0 0.0012696106006625414 6.348053003312707E-4 24-25 0.0 0.0 0.0 0.002124156197262329 6.592208888055503E-4 26-27 0.0 0.0 0.0 0.002856623851490718 8.057144196512282E-4 28-29 0.0 0.0 0.0 0.005078442402650166 8.301300081255079E-4 30-31 0.0 0.0 0.0 0.012158963060191262 8.301300081255079E-4 32-33 0.0 0.0 0.0 0.02744312144509032 8.789611850740671E-4 34-35 0.0 0.0 0.0 0.04968572254515907 8.789611850740671E-4 36-37 0.0 0.0 0.0 0.0864800143758985 8.789611850740671E-4 38-39 0.0 0.0 0.0 0.136727295455966 9.522079504969061E-4 40-41 0.0 0.0 0.0 0.19588626632914558 9.766235389711856E-4 42-43 0.0 0.0 0.0 0.2576332895805988 0.0010010391274454652 44-45 0.0 0.0 0.0 0.3278769376211013 0.0010742858928683044 46-47 0.0 0.0 0.0 0.40900993812113257 0.001147532658291143 48-49 0.0 0.0 0.0 0.4858457950496906 0.0011963638352397025 50-51 0.0 0.0 0.0 0.5680042502656416 0.001220779423713982 52-53 0.0 0.0 0.0 0.6420567301081317 0.0012696106006625414 54-55 0.0 0.0 0.0 0.7227990811925745 0.0012696106006625414 56-57 0.0 0.0 0.0 0.7920661056941058 0.001294026189136821 58-59 0.0 0.0 2.4415588474279644E-5 0.8418983217701106 0.00136727295455966 60-61 0.0 0.0 4.883117694855929E-5 0.8818666401025064 0.00136727295455966 62-63 4.883117694855929E-5 0.0 4.883117694855929E-5 0.9168541783861491 0.00136727295455966 64-65 9.766235389711857E-5 0.0 4.883117694855929E-5 0.9537217169823113 0.00136727295455966 66-67 9.766235389711857E-5 0.0 4.883117694855929E-5 0.9831425010938184 0.0014161041315082193 68-69 9.766235389711857E-5 0.0 4.883117694855929E-5 1.0253814691543222 0.0014161041315082193 70-71 9.766235389711857E-5 0.0 4.883117694855929E-5 1.0899118694918433 0.0014649353084567785 72-73 9.766235389711857E-5 0.0 4.883117694855929E-5 1.185938378961185 0.0014649353084567785 74-75 9.766235389711857E-5 0.0 4.883117694855929E-5 1.3091882695793489 0.0014649353084567785 76-77 9.766235389711857E-5 0.0 4.883117694855929E-5 1.5088589521220075 0.0014649353084567785 78-79 9.766235389711857E-5 0.0 4.883117694855929E-5 1.8140782236389774 0.0014649353084567785 80-81 9.766235389711857E-5 0.0 4.883117694855929E-5 2.1826315316582283 0.0015381820738796174 82-83 9.766235389711857E-5 0.0 4.883117694855929E-5 2.5490362678917435 0.0017579223701481342 84-85 9.766235389711857E-5 0.0 4.883117694855929E-5 2.8294981326957935 0.0017579223701481342 86-87 9.766235389711857E-5 0.0 4.883117694855929E-5 3.0432077786111633 0.0018067535470966934 88-89 9.766235389711857E-5 1.4649353084567786E-4 4.883117694855929E-5 3.2482498906181636 0.0018555847240452528 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5705 0.0 34.72793 1 GTATCAA 9025 0.0 33.99795 1 ATCAACG 11230 0.0 27.103018 3 TCAACGC 11220 0.0 27.085243 4 CAACGCA 11340 0.0 26.757143 5 TATCAAC 11520 0.0 26.462866 2 AACGCAG 11560 0.0 26.295887 6 TACATGG 8305 0.0 23.62168 2 GTACATG 8435 0.0 23.599764 1 ACGCAGA 13215 0.0 22.682707 7 CGCAGAG 13210 0.0 22.442877 8 ACATGGG 8325 0.0 22.094572 3 GCAGAGT 14245 0.0 20.284513 9 GAGTACT 6685 0.0 20.241516 12-13 AGTACTT 6855 0.0 20.08224 12-13 AGAGTAC 11655 0.0 19.148436 10-11 CATGGGG 3250 0.0 18.961866 4 GTACTTT 7195 0.0 18.545313 14-15 CATGGGA 5440 0.0 18.073425 4 GTATAGG 645 0.0 17.510717 1 >>END_MODULE