FastQCFastQC Report
Fri 27 May 2016
SRR939612_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939612_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050098
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29500.1438955601146872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78150.041.273951
GGTATCA53150.035.449411
GTACATG69700.030.411091
TATCAAC108900.029.8378312
ATCAACG116300.027.6963183
TCAACGC117550.027.479474
CAACGCA118850.026.8029295
AACGCAG121300.026.6110736
TACATGG81100.025.3905642
ACATGGG79350.025.058353
ACGCAGA127300.024.837827
CGCAGAG133300.023.2590358
GAGTACT59050.021.81747612-13
GCAGAGT135200.020.9077179
CATGGGA58200.020.6915044
GTCCTAT6451.6189006E-1020.6384981
GTACTTT64800.020.17626614-15
GTATAGG6702.6739144E-1019.8684021
AGTACTT63000.019.850312-13
CATGGGG31050.019.4680844
Produced by FastQC (version 0.11.5)