FastQCFastQC Report
Fri 27 May 2016
SRR939612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939612_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050098
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76550.3733967839586205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56540.27579169386048863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41600.20291712883969448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59900.035.1295171
GTATCAA94800.033.444531
TCAACGC117800.026.8233744
ATCAACG118400.026.6874453
TATCAAC120650.026.2717782
CAACGCA120750.026.2071195
CTCGCGA758.5829015E-725.95744164-65
AACGCAG123600.025.5865946
GTACATG85100.023.5626161
TACATGG84450.023.1790682
ACGCAGA139150.022.4224577
CGCAGAG138650.022.1973828
ACATGGG84850.021.7880383
GAGTACT67500.020.28386912-13
AGTACTT70000.019.76143612-13
GCAGAGT149250.019.6419439
CATGGGA57750.019.5177544
GTACTTT72800.018.61293614-15
AGAGTAC122550.018.07561110-11
GGACCGA2756.4145934E-717.1386176
Produced by FastQC (version 0.11.5)