##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939612_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2050098 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.451859374527462 31.0 31.0 34.0 30.0 34.0 2 31.650845471777448 31.0 31.0 34.0 30.0 34.0 3 31.705170191864 31.0 31.0 34.0 30.0 34.0 4 35.51778451566705 37.0 35.0 37.0 33.0 37.0 5 35.269374439660936 37.0 35.0 37.0 33.0 37.0 6 35.27586095884197 37.0 35.0 37.0 32.0 37.0 7 35.17718957825431 37.0 35.0 37.0 32.0 37.0 8 35.13437991744785 37.0 35.0 37.0 32.0 37.0 9 36.728453469053676 39.0 35.0 39.0 32.0 39.0 10-11 36.605425204063415 39.0 35.0 39.0 32.0 39.0 12-13 36.54495004629047 38.0 35.0 39.0 32.0 39.0 14-15 37.597888003402765 39.0 36.0 41.0 32.0 41.0 16-17 37.55584952524221 39.0 36.0 41.0 32.0 41.0 18-19 37.54076366105425 39.0 36.0 41.0 32.0 41.0 20-21 37.47790398312666 39.0 36.0 41.0 32.0 41.0 22-23 37.348441147691474 39.0 36.0 41.0 31.5 41.0 24-25 37.225479220993336 39.0 36.0 40.5 31.0 41.0 26-27 36.9444107062199 39.0 36.0 40.0 31.0 41.0 28-29 36.75107799724696 39.0 35.5 40.0 30.0 41.0 30-31 36.501646018873245 38.0 35.0 40.0 30.0 41.0 32-33 36.33111465890899 38.0 35.0 40.0 30.0 41.0 34-35 36.30085415428921 38.0 35.0 40.0 30.0 41.0 36-37 36.21684207291554 38.0 35.0 40.0 30.0 41.0 38-39 36.25963880751067 38.0 35.0 40.0 30.0 41.0 40-41 36.142702934201196 38.0 35.0 40.0 30.0 41.0 42-43 35.960232388890674 38.0 35.0 40.0 29.0 41.0 44-45 35.748310812458726 38.0 35.0 40.0 28.5 41.0 46-47 35.44749470513117 38.0 34.0 40.0 27.0 41.0 48-49 35.15479528295721 38.0 34.0 40.0 26.5 41.0 50-51 34.924947978096654 38.0 34.0 40.0 26.0 41.0 52-53 34.70610941525722 38.0 33.5 40.0 26.0 41.0 54-55 34.427763941040865 38.0 33.0 40.0 24.5 41.0 56-57 33.69587356311747 37.5 32.5 40.0 22.0 41.0 58-59 30.65686298898882 35.5 27.0 39.0 8.0 40.5 60-61 30.40814487892774 34.5 26.0 39.0 10.5 40.0 62-63 32.1575931979837 35.5 30.5 39.0 18.5 40.0 64-65 32.21214644373099 36.0 31.0 39.0 16.0 40.0 66-67 27.390737174515557 32.0 19.0 37.0 4.5 39.5 68-69 19.78591194177059 20.0 8.0 32.0 2.0 37.0 70-71 17.844045991947702 15.5 7.5 29.0 2.0 35.0 72-73 19.586074421808128 20.0 7.5 31.0 2.0 35.5 74-75 21.076588289925652 23.5 9.5 32.5 2.0 36.0 76-77 22.98061897528801 27.5 12.0 33.0 2.0 36.0 78-79 25.228040561963383 30.5 14.0 34.5 2.0 37.0 80-81 26.8276555559783 32.5 23.5 35.0 2.0 37.0 82-83 27.862083666244246 33.0 26.5 35.0 2.0 36.5 84-85 27.874326495611427 34.0 27.0 35.0 2.0 36.0 86-87 27.582807017030404 33.5 26.5 35.0 2.0 36.0 88-89 27.14453162726855 33.0 26.0 35.0 2.0 35.5 90-91 25.382564150591826 32.5 16.0 34.5 2.0 35.0 92-93 23.852319254981957 31.5 4.0 34.0 2.0 35.0 94-95 20.001614800853424 28.0 2.0 34.0 2.0 35.0 96-97 17.26982246702353 18.5 2.0 33.0 2.0 35.0 98-99 17.559533251581144 22.0 2.0 33.0 2.0 35.0 100-101 16.780812673345373 18.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24401631664536083 1101 2 0.22804793462503525 1101 3 0.2323312874334249 1101 4 0.2510758613082231 1101 5 0.3198028065031622 1101 6 0.31771266378508045 1101 7 0.3520783000990093 1101 8 0.41744467916091565 1101 9 0.5298747208699268 1101 10-11 0.3092423420686288 1101 12-13 0.3422339610043821 1101 14-15 0.4965103515764113 1101 16-17 0.43739094250909005 1101 18-19 0.49285686773633586 1101 20-21 0.35504731605394113 1101 22-23 0.4824559815340379 1101 24-25 0.45459864498338476 1101 26-27 0.4150554094162686 1101 28-29 0.4155672125123999 1101 30-31 0.4013362023428684 1101 32-33 0.40372534711138286 1101 34-35 0.27676863077953584 1101 36-37 0.3689186735948695 1101 38-39 0.28537204760865365 1101 40-41 0.26079321931523936 1101 42-43 0.6201081944241054 1101 44-45 0.6258963902971431 1101 46-47 0.5903058166410418 1101 48-49 0.3617678859691438 1101 50-51 0.27700258332378525 1101 52-53 0.2591194712807763 1101 54-55 0.38576795290929766 1101 56-57 -3.0883319781821292 1101 58-59 -9.89075027778308 1101 60-61 -11.565421183922087 1101 62-63 -3.989112132461454 1101 64-65 -1.1291045020396737 1101 66-67 -1.9024737357567574 1101 68-69 -4.623364608674688 1101 70-71 -4.648241753397835 1101 72-73 -3.5027747415247568 1101 74-75 -6.035524328080616 1101 76-77 -4.830419601532945 1101 78-79 1.3160695996544298 1101 80-81 1.92825462166914 1101 82-83 0.5150543724357988 1101 84-85 0.20118809662541892 1101 86-87 0.20236535990653337 1101 88-89 -0.01644661740970932 1101 90-91 1.3231988673159059 1101 92-93 1.0192240258424405 1101 94-95 -8.562147382329464 1101 96-97 -9.776335194092258 1101 98-99 -9.143863074745965 1101 100-101 -10.17959665389559 1102 1 0.24983891413237558 1102 2 0.19724418105506203 1102 3 0.26844469344143107 1102 4 0.10597342890734751 1102 5 0.31314875804064 1102 6 0.21522189049092333 1102 7 0.291511733784084 1102 8 0.2734978985317724 1102 9 0.21342809990051848 1102 10-11 0.3579217831415704 1102 12-13 0.2323520603985756 1102 14-15 0.39476713162387256 1102 16-17 0.38545586646427665 1102 18-19 0.4374022605257366 1102 20-21 0.48031919523484135 1102 22-23 0.3721391352552459 1102 24-25 0.25954935792005784 1102 26-27 0.11407188508107424 1102 28-29 0.18618975939642723 1102 30-31 0.19222628200189718 1102 32-33 0.06221875713020353 1102 34-35 0.03150950810675113 1102 36-37 -0.03268780846853048 1102 38-39 0.2256028647451629 1102 40-41 0.04193042784468304 1102 42-43 0.020230044296759786 1102 44-45 -0.07023390506727623 1102 46-47 -0.08638310883060996 1102 48-49 0.2230487923320581 1102 50-51 0.2437265444473482 1102 52-53 0.22101997946653995 1102 54-55 0.03492726810934954 1102 56-57 -3.474052926312851 1102 58-59 -9.802062546606962 1102 60-61 -8.161165570509365 1102 62-63 -2.5002594331135555 1102 64-65 -0.7234325948725591 1102 66-67 -2.1470383894090226 1102 68-69 -5.163604049471026 1102 70-71 -4.974238162375716 1102 72-73 -3.972129325671135 1102 74-75 -4.34598920029384 1102 76-77 -4.216241226824351 1102 78-79 1.5985004588516212 1102 80-81 1.7913989707516365 1102 82-83 0.5265276026838528 1102 84-85 0.24273552695784062 1102 86-87 0.1621066989249762 1102 88-89 0.04480607900939759 1102 90-91 1.2526215336294904 1102 92-93 0.7183909070836592 1102 94-95 -9.072766382123229 1102 96-97 -10.074384233428756 1102 98-99 -9.077764756074888 1102 100-101 -9.862788917171043 1103 1 0.2528870198445432 1103 2 0.27626188120744644 1103 3 0.3343743338952869 1103 4 0.25879712871403626 1103 5 0.2504263637418802 1103 6 0.3628762080446535 1103 7 0.38841217067526657 1103 8 0.39542848468913405 1103 9 0.35290446625334226 1103 10-11 0.40694011906102645 1103 12-13 0.5061085894282868 1103 14-15 0.6130129509587405 1103 16-17 0.5828270689978083 1103 18-19 0.5291450371191786 1103 20-21 0.43935890882177375 1103 22-23 0.40654416655684145 1103 24-25 0.4971682910631898 1103 26-27 0.5163975011294255 1103 28-29 0.34881351046183795 1103 30-31 0.30084648866592545 1103 32-33 0.3042007410810612 1103 34-35 0.2579850340549896 1103 36-37 0.2142767018219658 1103 38-39 0.2685988971613327 1103 40-41 0.23141364856115842 1103 42-43 0.22334719033841566 1103 44-45 0.2099184406151764 1103 46-47 0.3777009197073866 1103 48-49 0.32319303198445937 1103 50-51 0.06729889970603864 1103 52-53 0.2736479822241833 1103 54-55 0.3271759762108175 1103 56-57 0.7573214828708075 1103 58-59 -8.336679367811382 1103 60-61 -7.566900785448304 1103 62-63 -2.039292550617219 1103 64-65 -0.3163200501950243 1103 66-67 -1.687872813811353 1103 68-69 -3.188523237517014 1103 70-71 -3.5922076217154313 1103 72-73 -2.8662330828558 1103 74-75 -3.6576345654526143 1103 76-77 -0.3522561395355197 1103 78-79 3.6089191909334204 1103 80-81 2.965928552794395 1103 82-83 1.392551714149711 1103 84-85 0.9871366045830108 1103 86-87 0.9866932019875847 1103 88-89 1.0305020154644815 1103 90-91 2.3072206129593127 1103 92-93 1.737383852056034 1103 94-95 -7.161711088269705 1103 96-97 -8.643325323650062 1103 98-99 -8.002055826130459 1103 100-101 -9.418865658229079 1104 1 0.28172770178701967 1104 2 0.25675907148398025 1104 3 0.28402480271092756 1104 4 0.15809905707995853 1104 5 0.2643838334848425 1104 6 0.21120404610313415 1104 7 0.23594365622242464 1104 8 0.2803788290685034 1104 9 0.34432173198423044 1104 10-11 0.2843418579984771 1104 12-13 0.3632091846616632 1104 14-15 0.4463346266476904 1104 16-17 0.3964419426989565 1104 18-19 0.40066963904199326 1104 20-21 0.3851627512870408 1104 22-23 0.35789473603112043 1104 24-25 0.48237365054134784 1104 26-27 0.34402829791785905 1104 28-29 0.47608328354397145 1104 30-31 0.40761984424455733 1104 32-33 0.43010092258364807 1104 34-35 0.368864984756037 1104 36-37 0.382512659149036 1104 38-39 0.40867768038067 1104 40-41 0.2426776080123645 1104 42-43 0.37921402371269863 1104 44-45 0.3445276750546995 1104 46-47 0.4110534072439478 1104 48-49 0.1432657122783496 1104 50-51 0.2516624319524752 1104 52-53 0.27720313444064715 1104 54-55 0.16379590662160837 1104 56-57 0.5165480955886679 1104 58-59 -8.097182443051732 1104 60-61 -7.578981902838471 1104 62-63 -2.077776647344624 1104 64-65 -0.48057880234491535 1104 66-67 -1.8380288545186545 1104 68-69 -3.2869969117159066 1104 70-71 -3.906703852960824 1104 72-73 -2.8747209047454874 1104 74-75 -3.6724072116652344 1104 76-77 -0.3415557099529778 1104 78-79 3.5826108267552144 1104 80-81 2.9375901733192435 1104 82-83 1.3639156189123725 1104 84-85 1.0818640138515327 1104 86-87 1.0557425524619468 1104 88-89 0.9272681584948579 1104 90-91 2.391923075529359 1104 92-93 2.0505876699510814 1104 94-95 -6.215498468688034 1104 96-97 -7.639351090676829 1104 98-99 -5.443675300803317 1104 100-101 -4.909178849266498 1105 1 0.21255460678338522 1105 2 0.23271226566085446 1105 3 0.21032217606778048 1105 4 0.12483112070736979 1105 5 0.3060050948017121 1105 6 0.20651682201520316 1105 7 0.19425239775316783 1105 8 0.26103018438836045 1105 9 0.26384946596959935 1105 10-11 0.2631176078032027 1105 12-13 0.30277121725170986 1105 14-15 0.37244418094082477 1105 16-17 0.42136828660451187 1105 18-19 0.34243688021559393 1105 20-21 0.29275835705671227 1105 22-23 0.3389553360658013 1105 24-25 0.3011567898181866 1105 26-27 0.3579096082009201 1105 28-29 0.42595259567877264 1105 30-31 0.340004285309341 1105 32-33 0.12931043201496806 1105 34-35 0.16886790793257234 1105 36-37 0.29339226484235326 1105 38-39 0.3299642916753456 1105 40-41 0.25874622987979734 1105 42-43 0.18294946549813318 1105 44-45 0.38190287331367756 1105 46-47 0.31700785818787125 1105 48-49 0.1798929153989448 1105 50-51 0.2028086949795025 1105 52-53 0.4111184251595077 1105 54-55 0.28027196361838236 1105 56-57 0.7936154177362482 1105 58-59 -8.514240490576618 1105 60-61 -7.979318187453252 1105 62-63 -2.24081274201259 1105 64-65 -0.17281991628596316 1105 66-67 -1.2926401754444647 1105 68-69 -3.1485216644589222 1105 70-71 -3.6683661341998395 1105 72-73 -2.818603074084507 1105 74-75 -3.5566553865690267 1105 76-77 -0.060561388471963795 1105 78-79 3.8478745807979458 1105 80-81 3.251897471954141 1105 82-83 1.7011482775155784 1105 84-85 1.2283707383506943 1105 86-87 1.231472889227792 1105 88-89 1.191589436674679 1105 90-91 2.568390470378521 1105 92-93 2.204205866506264 1105 94-95 -6.312834281617462 1105 96-97 -7.774300231382242 1105 98-99 -5.422174343115003 1105 100-101 -4.752229921734232 1106 1 0.15281111624211974 1106 2 0.19025810600195925 1106 3 0.16304192605324985 1106 4 0.12106005479790838 1106 5 0.17424611266075374 1106 6 0.1928291677857814 1106 7 0.159598763702121 1106 8 0.2636608296110836 1106 9 0.3436981582644876 1106 10-11 0.2112022062103449 1106 12-13 0.21471366098740674 1106 14-15 0.18295212820363815 1106 16-17 0.22900142756243014 1106 18-19 0.18903897182210727 1106 20-21 0.19436555038699055 1106 22-23 0.26184443521564305 1106 24-25 0.15828567198941101 1106 26-27 0.1634512428774073 1106 28-29 0.3586734882899947 1106 30-31 0.41693056189293287 1106 32-33 0.3624437724312486 1106 34-35 0.4852089596694569 1106 36-37 0.3535268541285319 1106 38-39 0.4540313978854158 1106 40-41 0.4636527049595287 1106 42-43 0.3830139135998678 1106 44-45 0.38300911545812255 1106 46-47 0.38223815741550027 1106 48-49 0.33636929812645633 1106 50-51 0.26606746362985945 1106 52-53 0.3629036275546085 1106 54-55 0.2991006318980922 1106 56-57 1.0179909326695622 1106 58-59 -8.110870145523688 1106 60-61 -7.549505377616249 1106 62-63 -1.8625834711481417 1106 64-65 -0.11956293173546584 1106 66-67 0.8963217590810757 1106 68-69 -2.0251402339315696 1106 70-71 -2.3357333440480694 1106 72-73 -0.8263662137795258 1106 74-75 -0.7277939630177066 1106 76-77 2.5913434889363245 1106 78-79 4.867755968226259 1106 80-81 3.6825309677274305 1106 82-83 2.1590852166016 1106 84-85 1.7155060753551332 1106 86-87 1.4848480453365305 1106 88-89 1.6098062990243598 1106 90-91 2.95383241596949 1106 92-93 2.418365505356345 1106 94-95 -5.883652722394366 1106 96-97 -7.569410695228159 1106 98-99 -5.253937935851113 1106 100-101 -4.656479888157071 1107 1 -0.03207298187392027 1107 2 -0.005679282799665231 1107 3 -0.0427536596292164 1107 4 -0.06537261280323747 1107 5 0.01698344562973375 1107 6 0.07593704077831376 1107 7 0.052598647711768365 1107 8 0.0795233948065075 1107 9 -0.02610817716781355 1107 10-11 -0.006567417292842492 1107 12-13 0.11511589193403182 1107 14-15 0.03330471921183431 1107 16-17 0.08044865293562253 1107 18-19 0.019827922463299785 1107 20-21 0.14847966916504163 1107 22-23 0.1299694020404374 1107 24-25 -0.06293702358703257 1107 26-27 -0.026273093274426174 1107 28-29 -0.005754925020980295 1107 30-31 -0.11199084579742902 1107 32-33 0.005771673222142226 1107 34-35 0.022166336164310962 1107 36-37 -0.03239879802230661 1107 38-39 -0.008575475427569756 1107 40-41 -0.030595746372689803 1107 42-43 -0.058694152447785086 1107 44-45 -0.07648282714434629 1107 46-47 0.0060291545983091055 1107 48-49 0.01621391037464548 1107 50-51 -0.07018876102183214 1107 52-53 0.03270268901572848 1107 54-55 0.011813960762189879 1107 56-57 0.5277717698839837 1107 58-59 -8.39272099765958 1107 60-61 -7.826611536310708 1107 62-63 -2.1873184135363246 1107 64-65 -0.6468961562868891 1107 66-67 0.4155426381306597 1107 68-69 -2.677969022343543 1107 70-71 -2.4812257427481903 1107 72-73 -1.2239566616792175 1107 74-75 -1.204327640521278 1107 76-77 1.9921782262771544 1107 78-79 4.110806770034134 1107 80-81 3.0325852276205225 1107 82-83 1.4534954646440141 1107 84-85 1.0427554526442186 1107 86-87 0.8999660114555539 1107 88-89 0.7949314211582639 1107 90-91 2.060589148816188 1107 92-93 1.710854343742465 1107 94-95 -6.419259695970194 1107 96-97 -8.13928809932478 1107 98-99 -5.870809420507948 1107 100-101 -5.155029025798298 1108 1 -0.037183179341710115 1108 2 -0.0987387090323466 1108 3 -0.060138206732883503 1108 4 -0.03979180468330412 1108 5 -0.08627100851055758 1108 6 -0.12272764661469893 1108 7 -0.04669312810412407 1108 8 0.018514337034751804 1108 9 0.02743542236311214 1108 10-11 -0.08296225527692513 1108 12-13 -0.025917231595265378 1108 14-15 -0.1822115172352099 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0.29843180993445273 2312 86-87 0.21477980080079817 2312 88-89 0.34613465676527255 2312 90-91 2.0699943370080938 2312 92-93 3.3696731663052617 2312 94-95 5.53492304040785 2312 96-97 0.21938814606507862 2312 98-99 -1.0200781017390348 2312 100-101 -0.39615795112549357 2313 1 -0.3258679630746357 2313 2 -0.34920221015231334 2313 3 -0.29416401305502404 2313 4 -0.28150397241745395 2313 5 -0.32223109261227023 2313 6 -0.24207455385427323 2313 7 -0.34669278076772514 2313 8 -0.462120887197905 2313 9 -0.43360134589558896 2313 10-11 -0.4445145479286765 2313 12-13 -0.4008327130729725 2313 14-15 -0.5006446017213122 2313 16-17 -0.579794980291858 2313 18-19 -0.6106916564311646 2313 20-21 -0.562847343410013 2313 22-23 -0.6367807893424384 2313 24-25 -0.5821774906895669 2313 26-27 -0.6224378895888449 2313 28-29 -0.6692427302400432 2313 30-31 -0.6267541889235204 2313 32-33 -0.6872746116517305 2313 34-35 -0.6861160750550042 2313 36-37 -0.7643813714071257 2313 38-39 -0.8430258872000067 2313 40-41 -0.8554305691120234 2313 42-43 -0.8485036582538186 2313 44-45 -0.9169974474171667 2313 46-47 -0.9991068206135623 2313 48-49 -1.0191647729916014 2313 50-51 -0.9871985953341493 2313 52-53 -0.7506497873934634 2313 54-55 -0.926173294679991 2313 56-57 0.02040910732824841 2313 58-59 3.175497685024826 2313 60-61 3.123871388237987 2313 62-63 0.26702185516388965 2313 64-65 -0.4100526397756745 2313 66-67 -2.3784559357628545 2313 68-69 -5.071401941807217 2313 70-71 -3.747508513654317 2313 72-73 -3.0597742855284054 2313 74-75 -3.7976071855461946 2313 76-77 0.2762426067683421 2313 78-79 2.7625943157922173 2313 80-81 2.029638209027702 2313 82-83 0.42627044836904915 2313 84-85 0.19933661995623808 2313 86-87 0.22570723345988242 2313 88-89 0.2433945070067729 2313 90-91 1.9198010669753707 2313 92-93 3.2695836533717113 2313 94-95 5.504122991832578 2313 96-97 1.0933281395578227 2313 98-99 2.312132978465165 2313 100-101 3.6104253929005115 2314 1 -0.2911584443593789 2314 2 -0.3319542567355285 2314 3 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0.5252344312112669 2314 64-65 -0.4284107188973607 2314 66-67 -2.278341719978961 2314 68-69 -5.014422467844323 2314 70-71 -3.848945405719146 2314 72-73 -3.1149862996626805 2314 74-75 -4.258557602118309 2314 76-77 0.23586764555423656 2314 78-79 3.1311243071319623 2314 80-81 2.3293872005943754 2314 82-83 0.8331281423332726 2314 84-85 0.45165325721508864 2314 86-87 0.46950177935228155 2314 88-89 0.556219845428199 2314 90-91 2.1764508222189924 2314 92-93 3.7546190508094455 2314 94-95 6.498497010607263 2314 96-97 3.7223618728683086 2314 98-99 6.031667524542808 2314 100-101 6.415165994363646 2315 1 -0.3077553052501969 2315 2 -0.2978675839299889 2315 3 -0.36911001465202986 2315 4 -0.21761252624509098 2315 5 -0.34532996080259437 2315 6 -0.388417218284971 2315 7 -0.3759056902761344 2315 8 -0.33239629776500834 2315 9 -0.5760198440565105 2315 10-11 -0.46663606767595667 2315 12-13 -0.4879668130792325 2315 14-15 -0.5949510447756978 2315 16-17 -0.5051152775854177 2315 18-19 -0.5079089512047403 2315 20-21 -0.6348085126197205 2315 22-23 -0.4826464202797567 2315 24-25 -0.33383929818935343 2315 26-27 -0.4793762953645384 2315 28-29 -0.4185093249744938 2315 30-31 -0.37689856546699474 2315 32-33 -0.4792584438424541 2315 34-35 -0.31542486792134383 2315 36-37 -0.24029944274135318 2315 38-39 -0.5202081100945506 2315 40-41 -0.4092051195258293 2315 42-43 -0.2608718235608194 2315 44-45 -0.258708264155068 2315 46-47 -0.14700040032851547 2315 48-49 -0.36175307608858986 2315 50-51 -0.3558494250954922 2315 52-53 -0.34331677866114063 2315 54-55 -0.3493318831540506 2315 56-57 0.2786592518032691 2315 58-59 3.5573685213963735 2315 60-61 3.558629046399247 2315 62-63 1.007646243336282 2315 64-65 0.31658485219301014 2315 66-67 -1.5451960709843888 2315 68-69 -4.654339569170766 2315 70-71 -3.6490661651974925 2315 72-73 -3.26897176073129 2315 74-75 -0.6211692010821359 2315 76-77 2.1423375974734853 2315 78-79 4.076579616566175 2315 80-81 2.8567500557435395 2315 82-83 1.2326623899125053 2315 84-85 0.8178286946097408 2315 86-87 0.9846614351718372 2315 88-89 1.1338056197067523 2315 90-91 2.6536136595250674 2315 92-93 4.133578782780759 2315 94-95 6.834107793887014 2315 96-97 4.719430906505718 2315 98-99 6.728712979812418 2315 100-101 7.0529632331456025 2316 1 -0.4174298763116546 2316 2 -0.4180590886665847 2316 3 -0.5072177079529041 2316 4 -0.38315187780288795 2316 5 -0.44689640124266106 2316 6 -0.4882828984120877 2316 7 -0.49350659157289556 2316 8 -0.5165761239613431 2316 9 -0.6924351902581733 2316 10-11 -0.5814245815567247 2316 12-13 -0.5847260598050568 2316 14-15 -0.7158662108429539 2316 16-17 -0.7692897726364052 2316 18-19 -0.8616916695474188 2316 20-21 -0.692028778468945 2316 22-23 -0.9668794538055749 2316 24-25 -0.8459159042489475 2316 26-27 -0.8043295957808212 2316 28-29 -0.9035646251950311 2316 30-31 -0.5892413122979789 2316 32-33 -0.48000074335702436 2316 34-35 -0.6466570497134043 2316 36-37 -0.6176793514038366 2316 38-39 -0.7650067031719701 2316 40-41 -0.7667711809547839 2316 42-43 -0.6778985854939492 2316 44-45 -0.7026488760289524 2316 46-47 -0.651954408787546 2316 48-49 -0.5429148701022939 2316 50-51 -0.5551703288079253 2316 52-53 -0.5953063787627713 2316 54-55 -0.4759384633700918 2316 56-57 0.26558722094926424 2316 58-59 3.50602625978291 2316 60-61 3.5505231023804384 2316 62-63 0.9516974348264426 2316 64-65 0.27541112557664604 2316 66-67 -1.9223512802006084 2316 68-69 -3.170123611969867 2316 70-71 -3.32022883385223 2316 72-73 -0.6743239391080813 2316 74-75 5.779041754005046 2316 76-77 5.786409717874072 2316 78-79 4.70750213378307 2316 80-81 3.120489625128606 2316 82-83 1.4598981910524529 2316 84-85 1.1392879082241514 2316 86-87 0.9484583768436927 2316 88-89 0.8547632683384556 2316 90-91 2.429331333729614 2316 92-93 3.903115243858192 2316 94-95 6.742849726885346 2316 96-97 3.6384720500363947 2316 98-99 6.102077557267084 2316 100-101 6.482123452805451 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 12.0 4 23.0 5 73.0 6 211.0 7 444.0 8 1056.0 9 1838.0 10 3005.0 11 4829.0 12 6952.0 13 9479.0 14 11845.0 15 15849.0 16 21040.0 17 27334.0 18 29449.0 19 28392.0 20 29511.0 21 30537.0 22 30672.0 23 31438.0 24 33477.0 25 36017.0 26 45189.0 27 63610.0 28 88528.0 29 116740.0 30 142195.0 31 160567.0 32 169349.0 33 170290.0 34 172447.0 35 182775.0 36 183751.0 37 141096.0 38 56351.0 39 3721.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.587138488320576 20.582965942423552 14.686366235555633 23.143529333700236 2 15.337635512295261 21.841640265897297 39.811904885028596 23.00881933677885 3 18.45247095986818 26.430983164674398 30.84078095931211 24.27576491614531 4 12.519157620757642 18.010456085513958 38.4840139349436 30.9863723587848 5 12.673747958023753 38.73812578915336 35.09685568602837 13.491270566794524 6 30.058855722994704 38.15007867916558 18.200349446709378 13.590716151130335 7 26.768281321185622 33.7108762605495 22.161233267873047 17.359609150391837 8 25.216953149046116 35.80518385227626 20.609219953982667 18.368643044694956 9 26.072168257322332 16.515405605000346 20.92738981258457 36.48503632509275 10-11 23.940099448904395 27.28098851859765 28.6267046746058 20.15220735789216 12-13 25.256012151614215 24.748012046253397 28.68953093949655 21.306444862635836 14-15 22.269637496314186 25.976524439542764 27.07118396538297 24.68265409876008 16-17 21.3366977269856 29.129450947930756 27.609522866479004 21.92432845860464 18-19 21.16795388689128 27.953072292697467 29.900694579431775 20.978279240979482 20-21 22.39007598661137 26.982758872990466 29.661338140908384 20.96582699948978 22-23 21.760740218272492 27.14384873308496 29.47488363970893 21.620527408933622 24-25 21.650054034041812 27.18342770693965 29.739356171770453 21.42716208724809 26-27 21.65626797171061 27.35351058998672 29.560698835225846 21.42952260307683 28-29 21.444549914686174 27.454370028847684 29.669139418109946 21.4319406383562 30-31 21.7315376624783 27.312452240958496 29.501154753562737 21.454855343000467 32-33 21.507942566787243 27.277190690627336 29.474436200806974 21.740430541778448 34-35 21.86443708045568 27.286529485750826 29.475848710273546 21.373184723519945 36-37 21.964298050182112 27.069775979772686 29.375812708906007 21.590113261139194 38-39 21.639247268128692 27.450104554229437 29.41751077700622 21.493137400635646 40-41 21.878725566872003 27.239563042571906 29.448442792379804 21.433268598176287 42-43 21.671681445740546 27.314760342707217 29.17759250570845 21.835965705843783 44-45 21.786786218647812 27.368192354027492 29.064167239323258 21.78085418800143 46-47 21.92178880046868 27.47969047502807 28.626641605233612 21.971879119269637 48-49 21.786035163615303 27.568800632850373 28.64158062239887 22.003583581135455 50-51 21.875641438990655 27.623252082564015 28.52564157322759 21.97546490521774 52-53 22.05353072613701 27.67226560173991 28.37246600762772 21.901737664495357 54-55 21.88771757903413 27.654687487036885 28.374936495983818 22.08265843794517 56-57 21.801191816221312 27.743188401269524 28.437332510672945 22.018287271836215 58-59 21.056740660344378 27.270656981216156 29.8230003490909 21.849602009348562 60-61 21.311631080630626 27.604830779306532 29.319015380940517 21.764522759122322 62-63 21.86212679441358 27.59725039913797 28.56693737643552 21.97368543001293 64-65 21.818646548910937 27.586810746791684 28.480978389444616 22.113564314852763 66-67 21.786388726319668 27.311876168864497 28.865413153136753 22.036321951679085 68-69 21.276334710532137 26.81237405002642 30.25595408393375 21.65533715550769 70-71 22.189293462444937 26.81737735917397 29.211129443415423 21.782199734965673 72-73 21.358718777937554 27.02995415228932 29.55044800692746 22.060879062845668 74-75 21.38354073407162 27.267073808901422 29.190630622439407 22.158754834587555 76-77 20.262870844695218 27.66500873443032 29.501241790507844 22.570878630366625 78-79 21.444868673499574 27.491980501052502 28.625232956694465 22.437917868753463 80-81 21.411880901614005 27.787948013949286 28.208388261148244 22.59178282328847 82-83 21.74736865724206 27.745581422120157 27.959010659999006 22.54803926063878 84-85 21.727416114592966 27.62908256703967 27.822417098704022 22.821084219663344 86-87 21.532005103236642 27.659913324974276 28.002681857681548 22.805399714107534 88-89 21.756667242248906 27.498465926994708 27.876716137472453 22.86815069328393 90-91 21.48343040712263 27.51835152212778 28.21690596199954 22.78131210875005 92-93 21.34752138224641 27.53411556967224 28.190816340724012 22.927546707357337 94-95 20.31752629820167 27.593380563426827 28.906437577154815 23.182655561216688 96-97 20.155932277381165 27.58897039454592 28.778987712637743 23.476109615435178 98-99 20.77312793172262 27.31237588887498 28.437200077565617 23.477296101836785 100-101 20.047790071037504 27.483240236482597 28.919027449241142 23.549942243238757 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 399.0 1 311.0 2 235.5 3 272.5 4 282.5 5 314.0 6 411.5 7 643.5 8 944.0 9 1147.0 10 1266.5 11 1367.5 12 1505.0 13 1648.5 14 1847.0 15 2037.5 16 2210.0 17 2447.5 18 2776.0 19 3163.0 20 3594.0 21 4141.0 22 4953.5 23 6081.5 24 7343.5 25 9209.5 26 11964.0 27 15160.5 28 18665.0 29 22707.0 30 27542.5 31 32687.0 32 38173.5 33 44167.0 34 50038.5 35 55345.5 36 60889.5 37 65479.0 38 67978.0 39 69872.0 40 71600.5 41 73466.0 42 74954.5 43 76127.5 44 77384.0 45 78043.0 46 78772.0 47 79506.5 48 80066.5 49 78325.5 50 74240.0 51 70070.0 52 66067.5 53 62464.5 54 58327.5 55 53113.5 56 47407.0 57 41806.0 58 36244.0 59 30812.5 60 25504.0 61 20252.0 62 15492.5 63 11600.0 64 8396.0 65 6039.5 66 4277.5 67 2945.0 68 2020.0 69 1328.0 70 872.5 71 568.0 72 367.0 73 234.5 74 157.5 75 105.5 76 61.5 77 34.5 78 15.0 79 3.5 80 1.5 81 0.5 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024876859545251005 2 0.013852996295786837 3 1.9511262388432162E-4 4 0.0 5 0.0597532410645735 6 0.0 7 0.0 8 4.8778155971080404E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.146143257541835E-4 16-17 4.8778155971080404E-5 18-19 4.146143257541835E-4 20-21 0.0 22-23 0.0 24-25 7.316723395662061E-5 26-27 4.8778155971080404E-5 28-29 9.755631194216082E-4 30-31 0.004560757583296018 32-33 0.0048534265191225 34-35 0.001536511913089033 36-37 0.0867519503945665 38-39 0.07997178671458632 40-41 0.08545932926133287 42-43 0.08872746571139527 44-45 0.09245899464318291 46-47 0.08770312443600257 48-49 0.07748410076006122 50-51 0.07226483807115562 52-53 0.06126536389967699 54-55 0.04950982831064661 56-57 0.0712161077177774 58-59 0.7224776571656574 60-61 0.3470565797342371 62-63 0.0026827985784094225 64-65 0.0017072354589878142 66-67 1.1100201063558912 68-69 2.392836830239335 70-71 2.364301608996253 72-73 1.8164253611290777 74-75 1.2876945394805517 76-77 0.7479886327385326 78-79 0.5672411757876941 80-81 0.039876142506358236 82-83 1.9511262388432162E-4 84-85 0.00119506482129147 86-87 0.0012926211332336308 88-89 0.0 90-91 0.3320329076951443 92-93 0.05577782135293045 94-95 0.9601248330567611 96-97 1.672456633780434 98-99 0.6396279592487774 100-101 0.5759968547845029 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2050098.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.975608741325566 #Duplication Level Percentage of deduplicated Percentage of total 1 76.21730715194984 44.18744778758879 2 13.320284399841057 15.445031933767355 3 4.573389381366378 7.954351001874905 4 2.023017408703298 4.691426630554909 5 1.1309256055363603 3.2783050211061364 6 0.6785008945453633 2.3601901435680825 7 0.42022135309163045 1.7053812126113552 8 0.2924300148701625 1.356304650106605 9 0.20715882294771187 1.0809142979877073 >10 1.0353003316137017 10.596914734101459 >50 0.06713534096617119 2.6705413717635786 >100 0.032980566237819475 3.3210816502863247 >500 8.429552065854504E-4 0.32367158155544284 >1k 3.371820826341725E-4 0.3780159570181648 >5k 1.6859104131708625E-4 0.6504220261092122 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7655 0.3733967839586205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5654 0.27579169386048863 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4160 0.20291712883969448 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.8778155971080404E-5 7 0.0 0.0 0.0 0.0 9.755631194216081E-5 8 0.0 0.0 0.0 0.0 9.755631194216081E-5 9 0.0 0.0 0.0 0.0 9.755631194216081E-5 10-11 0.0 0.0 0.0 7.316723395662061E-5 9.755631194216081E-5 12-13 0.0 0.0 0.0 1.4633446791324122E-4 3.9022524776864323E-4 14-15 0.0 0.0 0.0 1.9511262388432162E-4 4.8778155971080403E-4 16-17 0.0 0.0 0.0 5.121706376963442E-4 5.853378716529649E-4 18-19 0.0 0.0 0.0 7.804504955372866E-4 5.853378716529649E-4 20-21 0.0 0.0 0.0 8.780068074794473E-4 6.828941835951257E-4 22-23 0.0 0.0 0.0 0.0014389556011468719 6.828941835951257E-4 24-25 0.0 0.0 0.0 0.0021706279407130782 6.828941835951257E-4 26-27 0.0 0.0 0.0 0.0028047439683371233 7.804504955372866E-4 28-29 0.0 0.0 0.0 0.004975371909050202 8.292286515083669E-4 30-31 0.0 0.0 0.0 0.012121371758813481 8.292286515083669E-4 32-33 0.0 0.0 0.0 0.026706040394166523 8.292286515083669E-4 34-35 0.0 0.0 0.0 0.04743675668187569 0.0010243412753926885 36-37 0.0 0.0 0.0 0.08209363649932833 0.0010243412753926885 38-39 0.0 0.0 0.0 0.13331070026896275 0.0011218975873348493 40-41 0.0 0.0 0.0 0.18784467864463064 0.0011706757433059298 42-43 0.0 0.0 0.0 0.2459882405621585 0.0011706757433059298 44-45 0.0 0.0 0.0 0.31876524927101046 0.00119506482129147 46-47 0.0 0.0 0.0 0.40159055810990496 0.0012194538992770102 48-49 0.0 0.0 0.0 0.48561093177009096 0.0012194538992770102 50-51 0.0 0.0 0.0 0.5754602950688211 0.0012194538992770102 52-53 0.0 0.0 0.0 0.6624317471652574 0.0012438429772625504 54-55 0.0 0.0 0.0 0.7620367416582037 0.0012926211332336308 56-57 0.0 0.0 0.0 0.857227313035767 0.0013657883671902515 58-59 0.0 0.0 0.0 0.9250289498355688 0.0014145665231613319 60-61 0.0 0.0 0.0 0.9835139588448942 0.0014145665231613319 62-63 2.4389077985540202E-5 0.0 0.0 1.0361943672936609 0.0015121228351034925 64-65 4.8778155971080404E-5 0.0 0.0 1.0987523523265716 0.0015121228351034925 66-67 4.8778155971080404E-5 0.0 0.0 1.1537741122619503 0.0015365119130890327 68-69 4.8778155971080404E-5 0.0 0.0 1.22169769445168 0.0016096791470456534 70-71 4.8778155971080404E-5 0.0 0.0 1.3264731734775608 0.0016584573030167338 72-73 4.8778155971080404E-5 0.0 0.0 1.4623691160129906 0.0017560136149588946 74-75 4.8778155971080404E-5 0.0 0.0 1.6490918970702864 0.001804791770929975 76-77 4.8778155971080404E-5 0.0 0.0 1.8857147316859975 0.0018535699269010555 78-79 4.8778155971080404E-5 0.0 0.0 2.180456739141251 0.001902348082872136 80-81 4.8778155971080404E-5 0.0 0.0 2.53078145532555 0.001902348082872136 82-83 4.8778155971080404E-5 0.0 0.0 2.883471912074447 0.0019511262388432163 84-85 4.8778155971080404E-5 2.4389077985540202E-5 0.0 3.1974812911382773 0.0019511262388432163 86-87 4.8778155971080404E-5 9.755631194216081E-5 0.0 3.4890283293774247 0.0019755153168287565 88-89 4.8778155971080404E-5 9.755631194216081E-5 0.0 3.7686734975596288 0.0019999043948142965 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5990 0.0 35.129517 1 GTATCAA 9480 0.0 33.44453 1 TCAACGC 11780 0.0 26.823374 4 ATCAACG 11840 0.0 26.687445 3 TATCAAC 12065 0.0 26.271778 2 CAACGCA 12075 0.0 26.207119 5 CTCGCGA 75 8.5829015E-7 25.957441 64-65 AACGCAG 12360 0.0 25.586594 6 GTACATG 8510 0.0 23.562616 1 TACATGG 8445 0.0 23.179068 2 ACGCAGA 13915 0.0 22.422457 7 CGCAGAG 13865 0.0 22.197382 8 ACATGGG 8485 0.0 21.788038 3 GAGTACT 6750 0.0 20.283869 12-13 AGTACTT 7000 0.0 19.761436 12-13 GCAGAGT 14925 0.0 19.641943 9 CATGGGA 5775 0.0 19.517754 4 GTACTTT 7280 0.0 18.612936 14-15 AGAGTAC 12255 0.0 18.075611 10-11 GGACCGA 275 6.4145934E-7 17.138617 6 >>END_MODULE