FastQCFastQC Report
Fri 27 May 2016
SRR939611_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939611_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731909
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA290601.063725036229245No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA226860.8304083335133052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113100.4139962202254907No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT111340.40755383872596046No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA103110.37742838432758924No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG95270.34873050310240933No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT91790.33599215786470193No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86750.31754351993422913No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC76220.27899904425806277No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG75570.2766197556360772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73770.2700309563751941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58140.21281821612652543No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG55200.202056510667083No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46460.17006422981146152No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG45430.1662939724566228No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT44030.16116935080926928No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG43870.16058367976385743No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA40690.1489434677362972No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT35300.12921367439398604No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34680.1269441990930152No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT33810.12375961278358832No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33450.1224418529314117No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA27450.1004791887284679No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG136350.023.7375161
TACATGG136600.023.5898442
ACATGGG139000.022.5679823
GAGTACT85550.021.60536612-13
AGTACTT89900.020.24323512-13
GTATAGC14800.020.2015571
CATGGGA107700.020.181614
CATGGGG32200.019.8968324
TAGCCCT22450.019.87094
TATCGCG757.426937E-419.11189590-91
GTACTTT97800.018.99621614-15
GTGTAGC18700.018.7800241
TCCGACG1151.9889612E-618.65598992-93
GCTTATA32250.018.5415841
GTAGCCC15700.017.5321183
ATGGGAG36650.017.2220045
ATGGGAT37900.017.1548675
ATAGCCC12050.016.9350873
GTCGGGA4255.820766E-1116.7497522
ACTTTTT120650.016.30404316-17
Produced by FastQC (version 0.11.5)