##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939611_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2731909 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.18720828548828 31.0 30.0 33.0 26.0 34.0 2 30.322839084317962 31.0 30.0 34.0 26.0 34.0 3 30.3683281544151 31.0 30.0 34.0 26.0 34.0 4 33.91064782904555 35.0 35.0 37.0 28.0 37.0 5 33.91232394636864 35.0 35.0 37.0 28.0 37.0 6 34.047905329203864 36.0 35.0 37.0 29.0 37.0 7 33.875602371821316 36.0 35.0 37.0 28.0 37.0 8 33.886817606296546 35.0 35.0 37.0 28.0 37.0 9 35.1234363955754 38.0 35.0 39.0 28.0 39.0 10-11 35.026032894946354 37.0 34.0 39.0 28.0 39.0 12-13 34.883784379347915 37.0 34.0 39.0 27.0 39.0 14-15 35.49411839852645 38.0 34.0 40.0 27.0 41.0 16-17 35.26216246588008 38.0 33.5 40.0 27.0 41.0 18-19 35.32405837822563 38.0 34.0 40.0 27.0 41.0 20-21 35.496309906369504 38.0 34.0 40.0 27.0 41.0 22-23 35.39734925284847 38.0 34.0 40.0 27.0 41.0 24-25 35.27717962055105 38.0 34.0 40.0 26.0 41.0 26-27 35.337339384291354 38.0 34.0 40.0 26.5 41.0 28-29 35.21385521992131 38.0 34.0 40.0 25.5 41.0 30-31 35.02656018190943 38.0 34.0 40.0 25.0 41.0 32-33 34.822399648011704 38.0 34.0 40.0 25.0 41.0 34-35 34.61300138474598 38.0 33.0 40.0 24.0 41.0 36-37 34.28968918803665 38.0 33.0 40.0 24.0 41.0 38-39 34.096119782906385 38.0 33.0 40.0 23.5 41.0 40-41 33.81949636682627 38.0 33.0 40.0 22.0 41.0 42-43 33.55808959961697 38.0 32.0 40.0 20.0 41.0 44-45 33.34368293380197 37.5 32.0 40.0 18.5 41.0 46-47 32.88442697029806 37.0 31.0 40.0 15.5 41.0 48-49 32.5318493405161 37.0 31.0 40.0 14.0 41.0 50-51 31.94714392024039 36.5 30.0 39.5 12.5 40.5 52-53 31.968557334816055 37.0 30.5 39.0 9.0 40.0 54-55 32.04510142907395 37.0 30.5 39.0 9.0 40.0 56-57 31.982873697476748 37.0 31.0 39.0 8.0 41.0 58-59 31.63635538372618 36.0 30.0 39.0 7.5 40.0 60-61 31.20577259345022 36.0 29.0 39.0 4.5 40.0 62-63 30.75875587364001 35.5 28.5 39.0 2.0 40.0 64-65 30.087889457518536 35.0 28.0 38.0 2.0 40.0 66-67 29.562651427994123 34.0 27.0 38.0 2.0 40.0 68-69 29.104518854764194 34.0 26.0 38.0 2.0 39.5 70-71 28.511823417251453 34.0 26.0 37.0 2.0 39.0 72-73 27.71694646490787 33.0 25.5 36.0 2.0 39.0 74-75 27.232148472002542 32.0 24.0 36.0 2.0 38.5 76-77 26.7872926953277 32.0 23.0 35.5 2.0 38.0 78-79 26.16762472688512 32.0 21.5 35.0 2.0 37.0 80-81 25.489644054761705 31.0 20.0 35.0 2.0 36.5 82-83 24.81063717715341 31.0 18.0 34.0 2.0 36.0 84-85 24.11813662168103 30.5 11.5 34.0 2.0 35.5 86-87 23.36466935758109 30.0 6.5 34.0 2.0 35.0 88-89 22.45984913845959 29.0 2.0 34.0 2.0 35.0 90-91 21.17904604435946 28.0 2.0 33.5 2.0 35.0 92-93 19.66965865261251 26.0 2.0 32.5 2.0 34.0 94-95 19.365945022326876 26.0 2.0 32.0 2.0 34.0 96-97 18.62734629887013 25.0 2.0 32.0 2.0 34.0 98-99 17.578471135019505 24.0 2.0 32.0 2.0 34.0 100-101 15.924428851766292 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9265263066550986 1101 2 0.9310537895968487 1101 3 0.8960428847785771 1101 4 0.9693661777346207 1101 5 0.7326783174634102 1101 6 0.897044939675574 1101 7 0.7336427656338032 1101 8 0.9044977443346838 1101 9 0.9381684909140375 1101 10-11 0.8330810131162565 1101 12-13 0.7617941553445391 1101 14-15 1.0320081478914531 1101 16-17 1.0125053735881195 1101 18-19 0.8366044611701255 1101 20-21 1.052956570598738 1101 22-23 1.2061381569932905 1101 24-25 1.061048997485365 1101 26-27 1.1054962574657878 1101 28-29 1.28858224000993 1101 30-31 1.2886829923489103 1101 32-33 0.975524586325264 1101 34-35 1.2804264450356442 1101 36-37 1.2287483734057645 1101 38-39 1.1028029441003895 1101 40-41 1.3308538198540774 1101 42-43 1.4988776869757388 1101 44-45 1.4716422640554114 1101 46-47 1.3315800570456489 1101 48-49 1.1889122696960754 1101 50-51 1.5193532918710382 1101 52-53 1.6369003984382076 1101 54-55 1.6469042454780478 1101 56-57 1.5217297313280511 1101 58-59 1.5952865785593424 1101 60-61 1.4501252408526817 1101 62-63 1.265096325655751 1101 64-65 1.3275283685568127 1101 66-67 1.2854148929217644 1101 68-69 1.1046211289110168 1101 70-71 1.1065863091326875 1101 72-73 1.3577055847373458 1101 74-75 1.398872882533336 1101 76-77 0.8105190046664745 1101 78-79 1.059612701774185 1101 80-81 1.1394837266269633 1101 82-83 1.0202798967031121 1101 84-85 0.9703832231466336 1101 86-87 0.3994386608670766 1101 88-89 -0.33192245747006766 1101 90-91 -3.923456790533198 1101 92-93 -8.996638771999272 1101 94-95 -6.99296142047368 1101 96-97 -3.5529013528578606 1101 98-99 -2.463905512020755 1101 100-101 -2.028736942076213 1102 1 0.9268098402019085 1102 2 0.8660761157243222 1102 3 0.783469797874119 1102 4 0.8503097568407654 1102 5 0.8633744465173834 1102 6 0.8790753593451583 1102 7 0.8842889311706159 1102 8 0.7471919633880262 1102 9 0.8718475982568705 1102 10-11 0.8165055678060043 1102 12-13 0.9116701593356851 1102 14-15 1.057965444430458 1102 16-17 1.2193003092339936 1102 18-19 0.920727495332784 1102 20-21 1.184218197898737 1102 22-23 1.2052986809085127 1102 24-25 1.1361192105462692 1102 26-27 0.9292279582848479 1102 28-29 1.2494507597423237 1102 30-31 1.1530673851107665 1102 32-33 1.0819460791949993 1102 34-35 1.0271049094362112 1102 36-37 1.0327533845902224 1102 38-39 0.9265121105631025 1102 40-41 1.1071599710487803 1102 42-43 1.1188033920586378 1102 44-45 0.8116386357387952 1102 46-47 0.8253615730544439 1102 48-49 0.8933758978187285 1102 50-51 0.8613274098791841 1102 52-53 1.0177260287298608 1102 54-55 1.0726680888465907 1102 56-57 1.098793521812162 1102 58-59 1.0776655676395954 1102 60-61 0.8265478290632764 1102 62-63 0.8904124484457476 1102 64-65 0.852098661201083 1102 66-67 0.8999876900172872 1102 68-69 1.0354266779534775 1102 70-71 1.0295868413998903 1102 72-73 0.9458735358623223 1102 74-75 1.003734798273097 1102 76-77 0.8391169569495496 1102 78-79 0.8433657446254017 1102 80-81 0.6168984540553808 1102 82-83 0.48626385794630167 1102 84-85 0.11612688257777748 1102 86-87 -0.29160133675969035 1102 88-89 -1.1509915432426716 1102 90-91 -4.799487309468049 1102 92-93 -10.088560690169324 1102 94-95 -8.59895419008299 1102 96-97 -5.162556811106832 1102 98-99 -3.9387911145067225 1102 100-101 -3.2410395311311344 1103 1 0.8324271680210629 1103 2 0.878586810317735 1103 3 0.7802436446692482 1103 4 0.8467397254924194 1103 5 0.8943495946242308 1103 6 0.6210594267176432 1103 7 0.8328067843600877 1103 8 0.7278761545296888 1103 9 0.8142474195278453 1103 10-11 0.670612022100542 1103 12-13 0.6772729772157362 1103 14-15 0.7940799954462321 1103 16-17 0.8915711281829104 1103 18-19 0.8447466001982633 1103 20-21 0.8800332126866763 1103 22-23 0.8280227416966071 1103 24-25 0.7925989294861253 1103 26-27 0.7839610930079104 1103 28-29 0.9846431074174475 1103 30-31 1.0603118580404285 1103 32-33 0.906798713678981 1103 34-35 0.9481633936563938 1103 36-37 0.8792192911193908 1103 38-39 0.7423514647124421 1103 40-41 0.6865615354668719 1103 42-43 0.6646781011269454 1103 44-45 0.4697504701003936 1103 46-47 0.5953555345475152 1103 48-49 0.6608482266639584 1103 50-51 0.6370827202776432 1103 52-53 0.6638471978414131 1103 54-55 0.8595739976536763 1103 56-57 0.6562723912500061 1103 58-59 0.5381719800150329 1103 60-61 0.4726919121520545 1103 62-63 0.7016612568346865 1103 64-65 0.44011739742110834 1103 66-67 0.6092673621856655 1103 68-69 0.6545839086106255 1103 70-71 0.6925693280449181 1103 72-73 0.6319024290397195 1103 74-75 0.6239011705962483 1103 76-77 0.2884175077948221 1103 78-79 0.4274717507495005 1103 80-81 0.14240011009568931 1103 82-83 0.03730255190351528 1103 84-85 -0.11309527887747706 1103 86-87 -0.46057271147364176 1103 88-89 -0.878170822537431 1103 90-91 -2.3694172297644727 1103 92-93 -8.952848279453804 1103 94-95 -5.5446982726326155 1103 96-97 -3.0426716404600427 1103 98-99 -2.1775173534705257 1103 100-101 -1.6254856076348378 1104 1 1.0495769080069444 1104 2 0.8696648115979535 1104 3 0.9552932226149373 1104 4 1.1262304309896365 1104 5 1.0847385095533184 1104 6 0.8369485257749147 1104 7 0.9654119525815901 1104 8 0.9435166713001237 1104 9 1.1922195568885812 1104 10-11 0.7577530413913323 1104 12-13 0.8060890200442827 1104 14-15 1.0749572951555777 1104 16-17 1.0801126667262722 1104 18-19 1.076059620702054 1104 20-21 1.1648734237710912 1104 22-23 1.0490325250532564 1104 24-25 1.1024798353007697 1104 26-27 0.9170951911473111 1104 28-29 0.9778687783957096 1104 30-31 1.1333655012114505 1104 32-33 1.0939455319919276 1104 34-35 1.149440988414085 1104 36-37 1.113811160003877 1104 38-39 0.7412827086248512 1104 40-41 0.953811871865561 1104 42-43 0.8795278085718152 1104 44-45 0.8271070837374026 1104 46-47 0.5697057578098637 1104 48-49 0.642794245524442 1104 50-51 0.6990172001921557 1104 52-53 0.5903296328988219 1104 54-55 0.7691397247679461 1104 56-57 0.8907761667089105 1104 58-59 0.6989831117736109 1104 60-61 0.7415768710030086 1104 62-63 0.705492674459208 1104 64-65 0.8120066560438275 1104 66-67 0.7381425033290618 1104 68-69 0.7192125329532963 1104 70-71 0.8658335828354815 1104 72-73 0.81050510848344 1104 74-75 0.7975938881694624 1104 76-77 0.7563079158797663 1104 78-79 0.4908838732174594 1104 80-81 0.5665010677469482 1104 82-83 0.537303659996919 1104 84-85 0.5001256420737228 1104 86-87 0.45340618230193996 1104 88-89 0.4625429669093073 1104 90-91 -0.10056089665977908 1104 92-93 -3.477608626552982 1104 94-95 -1.3897210679607461 1104 96-97 -0.4226503502921162 1104 98-99 -0.13674265582304557 1104 100-101 -0.08239554882093358 1105 1 0.878331109436008 1105 2 0.8785637652374696 1105 3 0.8914183155985107 1105 4 0.8095193189995058 1105 5 0.7351400557451342 1105 6 0.7597740836488782 1105 7 0.8750599390271461 1105 8 0.7867823532474105 1105 9 1.0377531040429275 1105 10-11 0.925292682334657 1105 12-13 0.8603546173861361 1105 14-15 0.9748218165799827 1105 16-17 1.1689088192665835 1105 18-19 1.1158346141918258 1105 20-21 0.8576309786519971 1105 22-23 1.0215571842416793 1105 24-25 0.9595746604147379 1105 26-27 0.968524308822488 1105 28-29 0.866782389652407 1105 30-31 1.1037722775575247 1105 32-33 1.1835597202137507 1105 34-35 1.1466748301491734 1105 36-37 0.9800181005064701 1105 38-39 1.1177571231717138 1105 40-41 0.9962156771324189 1105 42-43 0.9939973160033659 1105 44-45 1.042174652715559 1105 46-47 1.0841612009610557 1105 48-49 0.8978252473736745 1105 50-51 0.783683069150424 1105 52-53 0.8224133329472849 1105 54-55 0.8226180520415554 1105 56-57 0.7836090973423424 1105 58-59 0.6639921711479495 1105 60-61 0.709128488560065 1105 62-63 0.9329966932174436 1105 64-65 0.806712773016887 1105 66-67 0.834519083225711 1105 68-69 0.9368028822199186 1105 70-71 0.841098029763149 1105 72-73 0.9047517190468142 1105 74-75 0.7062299056926769 1105 76-77 0.4130609130548244 1105 78-79 0.28207216206701347 1105 80-81 0.35142196166381723 1105 82-83 0.42105963594260487 1105 84-85 0.15047334844231486 1105 86-87 -0.0949859298343263 1105 88-89 -0.0964995412792895 1105 90-91 -1.2627352344024843 1105 92-93 -7.426727796057227 1105 94-95 -3.1077267530065704 1105 96-97 -1.161001994659209 1105 98-99 -0.6816104570815789 1105 100-101 -0.4635084289548086 1106 1 0.8048161436368879 1106 2 0.854745752724476 1106 3 0.7363567810945106 1106 4 0.8559383433229755 1106 5 0.6949489460180089 1106 6 0.5891262150548684 1106 7 0.7320811605709139 1106 8 0.7184287340618241 1106 9 0.8055767487569483 1106 10-11 0.7015277725717226 1106 12-13 0.7601897431253235 1106 14-15 0.5975989851052432 1106 16-17 0.8707293560406058 1106 18-19 0.9141885793726772 1106 20-21 0.8014037410028934 1106 22-23 0.9385664614275058 1106 24-25 0.7913382283178123 1106 26-27 0.8638156142625846 1106 28-29 0.7899970165266055 1106 30-31 0.881831364521183 1106 32-33 0.8408596935871628 1106 34-35 0.7820830819855829 1106 36-37 0.9346987393641442 1106 38-39 0.8786240067623581 1106 40-41 0.9125893760774275 1106 42-43 0.8125295740436158 1106 44-45 0.6813151403281736 1106 46-47 0.7658809177263279 1106 48-49 0.7753399183246685 1106 50-51 0.7131918010176399 1106 52-53 0.6426883540454682 1106 54-55 0.922772396011986 1106 56-57 0.8196512726596481 1106 58-59 0.8488860182992788 1106 60-61 0.6763717638414128 1106 62-63 0.6847323107211913 1106 64-65 0.584086638942221 1106 66-67 0.33989855728284013 1106 68-69 0.5655484689000048 1106 70-71 0.4490320018338103 1106 72-73 0.5239433236975941 1106 74-75 0.5405876858472958 1106 76-77 0.37948433001370674 1106 78-79 0.2301247352939022 1106 80-81 0.2955474887424572 1106 82-83 0.4017764177655181 1106 84-85 0.20566034383456255 1106 86-87 0.16122882189903365 1106 88-89 0.26498652338571915 1106 90-91 -0.22759264674909474 1106 92-93 -3.74560604935283 1106 94-95 -1.247241065927689 1106 96-97 -0.40297528081683964 1106 98-99 -0.2723349260164696 1106 100-101 -0.3446974190849179 1107 1 0.6688161672590134 1107 2 0.5732157474537871 1107 3 0.6317535664180802 1107 4 0.6161353518756627 1107 5 0.4696056500519248 1107 6 0.5959293880972965 1107 7 0.555211948915165 1107 8 0.38600928182945893 1107 9 0.5790051021026557 1107 10-11 0.4202982112494098 1107 12-13 0.5038394505800241 1107 14-15 0.5786975912520234 1107 16-17 0.6627852341858542 1107 18-19 0.4714995543612659 1107 20-21 0.4779540539222822 1107 22-23 0.5269390591677592 1107 24-25 0.6595548018985298 1107 26-27 0.5655456399073557 1107 28-29 0.573967482960299 1107 30-31 0.5075859808018421 1107 32-33 0.48082597299855934 1107 34-35 0.4157924500788752 1107 36-37 0.44746207073389144 1107 38-39 0.3423618247568143 1107 40-41 0.06012211856226912 1107 42-43 -0.017501090432098465 1107 44-45 0.07868068243388393 1107 46-47 0.1206232815041588 1107 48-49 -0.07210320770971634 1107 50-51 -0.05179237953809945 1107 52-53 0.11741267642256759 1107 54-55 0.22763737340118695 1107 56-57 0.1972760971546137 1107 58-59 0.1687658111922694 1107 60-61 0.19381301486934532 1107 62-63 0.010742784182465215 1107 64-65 0.15022712269388805 1107 66-67 0.0828536265178208 1107 68-69 0.04357767470927598 1107 70-71 0.13213597906208108 1107 72-73 0.19534475076943636 1107 74-75 0.021647167843539705 1107 76-77 -0.04965445146831726 1107 78-79 -0.013767098979151626 1107 80-81 -0.1800619252663509 1107 82-83 -0.1796593942673006 1107 84-85 -0.20382482663600499 1107 86-87 -0.21906530312993766 1107 88-89 -0.20983524027193212 1107 90-91 -0.5452231700825934 1107 92-93 -4.097775815685688 1107 94-95 -1.6647673566186505 1107 96-97 -0.8861747800277158 1107 98-99 -0.5448899987947726 1107 100-101 -0.35754859524546667 1108 1 0.39191622550334415 1108 2 0.466534426434837 1108 3 0.17158454315591953 1108 4 -0.2862817009334222 1108 5 -0.8196881568505674 1108 6 -0.25923966007540855 1108 7 0.014135444923624618 1108 8 0.19470440607109651 1108 9 0.4113710373146162 1108 10-11 0.28583809865143195 1108 12-13 0.15838991778019107 1108 14-15 0.2736451997412175 1108 16-17 0.3354386000118126 1108 18-19 0.3601835395340487 1108 20-21 0.22855263114781366 1108 22-23 0.14313342769297321 1108 24-25 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0.23708156284570947 2311 72-73 0.3252539103415728 2311 74-75 0.4437234615346668 2311 76-77 0.3887035695700156 2311 78-79 0.42169303760915966 2311 80-81 0.4529900981943058 2311 82-83 0.6089595924831777 2311 84-85 0.5786985244664749 2311 86-87 0.7526451156177565 2311 88-89 1.1212580288779996 2311 90-91 1.9274626982059573 2311 92-93 2.8524593778144194 2311 94-95 1.9750452387997655 2311 96-97 1.9030883465343678 2311 98-99 1.6815424937863384 2311 100-101 1.7495001930082328 2312 1 0.2660254685405583 2312 2 0.10447701645009744 2312 3 0.07147251000421662 2312 4 0.054341717676607004 2312 5 0.10515633465134755 2312 6 0.23228411716158348 2312 7 0.2656152001100338 2312 8 -0.48052494870256623 2312 9 0.1530751170466118 2312 10-11 0.1485963339672054 2312 12-13 0.13494471429908828 2312 14-15 -0.19582057410347176 2312 16-17 0.1360475570595483 2312 18-19 0.11227961441768741 2312 20-21 0.37680315024207545 2312 22-23 0.19251823437122795 2312 24-25 0.3119857625487228 2312 26-27 0.41049575469468635 2312 28-29 0.25690297477816415 2312 30-31 0.49748216995207173 2312 32-33 0.325414806499964 2312 34-35 0.24326043392391483 2312 36-37 0.2768354514075355 2312 38-39 0.4803290759943337 2312 40-41 0.5637857173122072 2312 42-43 0.5435152671140386 2312 44-45 0.5279041072435504 2312 46-47 0.6098031370702088 2312 48-49 0.3319537712536942 2312 50-51 0.3661363866565672 2312 52-53 0.491631962603023 2312 54-55 0.4864808243501457 2312 56-57 0.3765663404835635 2312 58-59 0.6127198491195109 2312 60-61 0.6638642665311103 2312 62-63 0.35941003334366073 2312 64-65 0.4842391912172417 2312 66-67 0.42223345503547094 2312 68-69 0.33348946438722393 2312 70-71 0.20300384539810423 2312 72-73 0.18605699342386117 2312 74-75 0.40055418813713217 2312 76-77 0.42005701172088905 2312 78-79 0.7166138625408571 2312 80-81 0.5163001214555436 2312 82-83 0.8047130296113849 2312 84-85 0.6597277951224676 2312 86-87 0.7134417634040666 2312 88-89 0.8986307957518171 2312 90-91 1.5649743000505332 2312 92-93 2.569162737876031 2312 94-95 1.9176782898520663 2312 96-97 1.4736038608296198 2312 98-99 1.6534788481859373 2312 100-101 1.4856689630923707 2313 1 -0.2566763552617388 2313 2 -0.7937193183788835 2313 3 -0.3062858209280144 2313 4 -0.22236991119189042 2313 5 -0.8360726083959378 2313 6 -0.3385408301659112 2313 7 -1.1385768566509498 2313 8 -0.9386142354305989 2313 9 -1.1020418014590803 2313 10-11 -1.8695423958300665 2313 12-13 -0.5478169424721813 2313 14-15 -0.16716094614756827 2313 16-17 -1.3691048691962706 2313 18-19 -1.0879470889099707 2313 20-21 -1.324548554290601 2313 22-23 -1.0773946511395778 2313 24-25 -0.2670579703601632 2313 26-27 -0.7996421096346396 2313 28-29 -2.3103570051489797 2313 30-31 -0.6063631640724978 2313 32-33 -0.17736882972930346 2313 34-35 -0.026371120951360183 2313 36-37 -0.018848616377468375 2313 38-39 -0.2693371083096352 2313 40-41 -0.22186770562419156 2313 42-43 -0.014855778698994015 2313 44-45 -0.5096434908151508 2313 46-47 -0.7050305334885607 2313 48-49 -0.20139309394217264 2313 50-51 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-1.6664075233791777 2314 9 -1.6999564573275876 2314 10-11 -1.1129168337351842 2314 12-13 -2.0260967864313884 2314 14-15 -2.793125486382671 2314 16-17 -1.8660857789713603 2314 18-19 -2.272844587491349 2314 20-21 -2.0302038116241974 2314 22-23 -3.30955429979921 2314 24-25 -1.986261578567813 2314 26-27 -2.0703098646324207 2314 28-29 -1.419979220818547 2314 30-31 -1.7081110155543016 2314 32-33 -2.524067792028873 2314 34-35 -0.931378801271741 2314 36-37 -0.587843565881073 2314 38-39 -0.7820297806325343 2314 40-41 -0.9177932356605893 2314 42-43 -0.853949316600854 2314 44-45 -1.5145476239589897 2314 46-47 -1.6010149088367953 2314 48-49 -2.0164740587481376 2314 50-51 -1.1795997630842514 2314 52-53 -0.8120495314829057 2314 54-55 -0.5151240892068571 2314 56-57 -0.5293029557482143 2314 58-59 -0.8423113717307373 2314 60-61 -0.7539170299221531 2314 62-63 -0.9969353489094388 2314 64-65 -1.157532698517361 2314 66-67 -1.005796788299751 2314 68-69 -0.931861887309477 2314 70-71 -1.1438467034158855 2314 72-73 -0.8586712936035141 2314 74-75 -0.5943291915518536 2314 76-77 -0.8280724525838359 2314 78-79 -0.6615170605825931 2314 80-81 -0.5386847259967382 2314 82-83 -0.5522881821664996 2314 84-85 -0.8135766209454083 2314 86-87 -0.6208957412279474 2314 88-89 -0.4724998739418105 2314 90-91 0.17076006027610902 2314 92-93 1.0764517735223187 2314 94-95 0.38816100977165036 2314 96-97 0.3747065467597892 2314 98-99 0.31279702613200655 2314 100-101 0.3653543800163721 2315 1 -6.883258825501624 2315 2 -2.965451289287703 2315 3 -1.4323995393419118 2315 4 -0.9389929849486691 2315 5 -1.394626550131342 2315 6 -1.741273744205138 2315 7 -3.041487446243792 2315 8 -2.6686826524214844 2315 9 -3.243876451957636 2315 10-11 -2.494141543766503 2315 12-13 -2.781458023137745 2315 14-15 -4.763688005853048 2315 16-17 -4.592572347211892 2315 18-19 -3.3572777995992027 2315 20-21 -4.268104320392528 2315 22-23 -5.673947680475305 2315 24-25 -3.717257958154434 2315 26-27 -4.597856567706888 2315 28-29 -3.0970780623206906 2315 30-31 -2.8698754873112904 2315 32-33 -1.9292836571329701 2315 34-35 -2.6146608303555254 2315 36-37 -3.2159700127125426 2315 38-39 -2.377743975372397 2315 40-41 -3.710866645630446 2315 42-43 -7.8250661500170935 2315 44-45 -4.40427626076621 2315 46-47 -2.929696293526586 2315 48-49 -5.720145439324408 2315 50-51 -6.831029878004625 2315 52-53 -7.034862115926227 2315 54-55 -4.8320561266381645 2315 56-57 -4.158730044908314 2315 58-59 -4.5040189383688904 2315 60-61 -3.5609342378579747 2315 62-63 -3.59514164362675 2315 64-65 -2.274350516940764 2315 66-67 -2.855625685687233 2315 68-69 -2.314708554584275 2315 70-71 -2.1261828812163053 2315 72-73 -2.6211188849572054 2315 74-75 -3.035999185751617 2315 76-77 -2.814065940658935 2315 78-79 -1.905794707649516 2315 80-81 -1.6490527956202072 2315 82-83 -2.0115042081969854 2315 84-85 -2.079839329396716 2315 86-87 -2.559041275477732 2315 88-89 -2.119489023028489 2315 90-91 -1.1237354398090886 2315 92-93 -0.2868551389113527 2315 94-95 -0.4505383651110968 2315 96-97 -0.5244721047631558 2315 98-99 -0.7116570074405111 2315 100-101 -0.575787977873583 2316 1 -4.357492052162641 2316 2 -2.916629643916142 2316 3 -4.501393790102146 2316 4 -4.341440668171245 2316 5 -4.335590856175408 2316 6 -4.351407344365448 2316 7 -3.2171488404687345 2316 8 -1.8827508035043934 2316 9 -2.0565092160345912 2316 10-11 -3.2371185560285305 2316 12-13 -4.196435430332496 2316 14-15 -3.2732895826151704 2316 16-17 -3.043378090918999 2316 18-19 -3.284941239557625 2316 20-21 -5.727355967875944 2316 22-23 -6.516602471722102 2316 24-25 -4.336666685852599 2316 26-27 -3.790202969579717 2316 28-29 -4.484169812432636 2316 30-31 -6.368818659956936 2316 32-33 -4.455092656336134 2316 34-35 -6.122375400156621 2316 36-37 -9.428027121872532 2316 38-39 -6.049268303205235 2316 40-41 -5.407132124980034 2316 42-43 -4.7515781901796785 2316 44-45 -3.561028676861561 2316 46-47 -5.866426824142081 2316 48-49 -3.455014748306855 2316 50-51 -4.5708016604218855 2316 52-53 -5.633183157091217 2316 54-55 -7.337108049270032 2316 56-57 -5.129640472773829 2316 58-59 -5.995399745493515 2316 60-61 -6.271643605387624 2316 62-63 -6.4377109710738765 2316 64-65 -5.245933241288299 2316 66-67 -6.653660501839983 2316 68-69 -5.491620250462695 2316 70-71 -6.3515887611707065 2316 72-73 -8.407208504546588 2316 74-75 -8.323170990704334 2316 76-77 -7.529108097927171 2316 78-79 -6.788327076501378 2316 80-81 -7.034862869868007 2316 82-83 -5.752990664321334 2316 84-85 -7.143729704531065 2316 86-87 -5.689306548364769 2316 88-89 -5.4597267068515585 2316 90-91 -6.0086885145604505 2316 92-93 -4.807431223396367 2316 94-95 -4.979144223384678 2316 96-97 -5.595950428332252 2316 98-99 -6.521471602723311 2316 100-101 -5.666593152571709 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14151.0 3 5530.0 4 5485.0 5 5968.0 6 6622.0 7 7817.0 8 9755.0 9 11947.0 10 14720.0 11 18274.0 12 22401.0 13 25702.0 14 27839.0 15 31808.0 16 35314.0 17 39682.0 18 36754.0 19 34779.0 20 36865.0 21 39830.0 22 44542.0 23 49932.0 24 56238.0 25 62763.0 26 71053.0 27 80571.0 28 91679.0 29 104035.0 30 119346.0 31 136415.0 32 157521.0 33 183133.0 34 216217.0 35 257472.0 36 299074.0 37 276272.0 38 92667.0 39 1736.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.85021680231696 19.476340428182287 13.739899503378892 21.933543266121866 2 13.826922189073976 22.2503383832499 41.72487151696093 22.19786791071519 3 20.124540621347414 27.593863470649232 30.302832667089252 21.978763240914105 4 11.328859965359673 16.850280431292962 41.14280835808733 30.67805124526004 5 12.036620767963313 39.97800145166969 35.20306338212658 12.78231439824041 6 27.82656733159911 38.599601209048004 19.432761449670686 14.141070009682203 7 25.83288177037593 34.80500723783061 22.56206380209049 16.800047189702973 8 23.251622906177175 34.944973781075554 21.78700480707759 20.016398505669684 9 25.90287470646635 18.318844402436923 22.066000652093475 33.71228023900325 10-11 22.910085369631286 26.905731432338754 29.594665071248826 20.58951812678113 12-13 26.78271988041744 24.553351444131607 28.554952472893337 20.108976202557617 14-15 23.08999456101691 26.031716837270718 28.79268314800061 22.08560545371176 16-17 20.160681517961674 29.35790959049606 29.696257817318706 20.785151074223563 18-19 19.612262028329624 29.969033135497618 31.060897225695754 19.357807610477 20-21 21.52640344143388 28.255813740452425 29.58363486796905 20.634147950144648 22-23 20.426570069552856 28.298098839808567 29.072433177224188 22.202897913414393 24-25 21.02176791580568 28.02787539460361 30.240899490050722 20.70945719953999 26-27 21.88435820631817 27.086748848583795 30.769117981007337 20.259774964090695 28-29 20.3435914570233 28.007282083219774 31.330149514371996 20.318976945384925 30-31 20.42917691909403 29.163136165981644 30.476418271543842 19.931268643380484 32-33 20.26782403873416 28.774112859904815 29.954791451814415 21.003271649546605 34-35 21.213671197488818 27.702604135358737 29.906944898536658 21.176779768615788 36-37 21.71514552822135 27.51129422157384 30.719073076591773 20.054487173613033 38-39 22.087629508326504 26.95811018635125 31.178282038237853 19.775978267084398 40-41 20.892538121381126 27.805559744891724 31.469969545409484 19.83193258831767 42-43 20.035554372975305 28.972232698075167 30.404266524757034 20.587946404192493 44-45 20.5996207019762 29.11550266149942 29.27445968355184 21.01041695297254 46-47 20.70548532424409 28.438842800418 28.496463061637183 22.35920881370072 48-49 21.283156090250262 28.098576678407923 29.168780776799828 21.44948645454199 50-51 21.92922762844505 28.792964121035773 29.188048509345194 20.089759741173985 52-53 20.79736592329181 29.079585703837974 29.820406027546042 20.30264234532417 54-55 19.917962805331793 28.339388468336534 30.377466794534303 21.36518193179737 56-57 21.10604762406587 29.487608304200208 28.477908463191465 20.928435608542458 58-59 21.000910244937803 28.678634790195595 28.853225157066277 21.467229807800326 60-61 20.848688336110826 27.8956313054773 29.719576570260152 21.536103788151724 62-63 22.46181468414665 27.60586732566912 29.149900396489954 20.782417593694277 64-65 21.715871678357104 28.236829728178293 29.67539221737968 20.371906376084922 66-67 20.496882800557508 28.33356727752366 29.87395233622288 21.295597585695955 68-69 20.340474141659058 29.539759059104988 29.32567773788809 20.79408906134787 70-71 20.785553621582118 29.03864782636901 28.396240003782424 21.779558548266444 72-73 20.706201655683707 28.284141441971055 28.364760747820135 22.644896154525103 74-75 20.902924497238597 28.921238263767222 28.60230753604929 21.573529702944885 76-77 21.642744844592954 27.648437679046978 29.12683286809767 21.581984608262403 78-79 20.821462279198393 27.86524162682759 29.704200831468796 21.609095262505214 80-81 20.582858856907542 29.431130665068846 28.92743453435427 21.058575943669346 82-83 20.67343383674268 28.933034865477698 28.577154301268575 21.81637699651105 84-85 21.040690804801233 28.102493770431465 28.402231609804097 22.454583814963208 86-87 21.326914658906183 28.20134503271295 28.976806544496288 21.49493376388458 88-89 22.07835710068827 27.19617326640094 29.165863013497706 21.55960661941308 90-91 20.744055663793155 27.73267520957693 29.896405698136192 21.626863428493728 92-93 20.167361198897506 28.880138770281416 29.609485015713226 21.343015015107845 94-95 20.531805960311647 29.217994424526218 28.22711484357597 22.02308477158617 96-97 20.218072317435077 28.458468597850338 28.02801204068131 23.295447044033278 98-99 21.128745705850754 28.176478323521586 27.95771536496165 22.737060605666013 100-101 21.719871814624227 28.317214785337917 28.216538055767092 21.746375344270763 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3891.0 1 2467.0 2 1075.0 3 1091.0 4 906.5 5 910.0 6 1404.0 7 2497.5 8 3667.5 9 3877.5 10 3207.0 11 2603.0 12 2541.0 13 2655.0 14 2843.5 15 2998.0 16 3125.5 17 3484.5 18 4000.0 19 4505.0 20 5179.0 21 6273.5 22 7760.5 23 9669.5 24 12097.5 25 15110.5 26 19376.0 27 24518.5 28 29442.5 29 35103.5 30 41333.0 31 47692.0 32 54708.0 33 62476.5 34 70809.0 35 78053.5 36 84475.5 37 89972.0 38 92391.5 39 92611.0 40 93156.5 41 93650.0 42 93627.5 43 93765.5 44 95118.0 45 97843.5 46 104963.0 47 125423.0 48 182011.5 49 179713.0 50 117661.5 51 89407.0 52 72982.5 53 62493.5 54 54599.0 55 47236.0 56 40349.5 57 34076.5 58 28541.5 59 23707.5 60 18991.0 61 14641.0 62 10703.5 63 7473.5 64 5117.5 65 3577.0 66 2539.5 67 1822.0 68 1335.5 69 1007.5 70 751.0 71 586.5 72 466.5 73 380.5 74 293.0 75 231.5 76 187.0 77 148.0 78 121.0 79 90.0 80 57.0 81 37.0 82 35.0 83 32.5 84 27.0 85 17.5 86 9.5 87 5.0 88 6.0 89 7.0 90 6.5 91 5.5 92 3.0 93 2.5 94 1.5 95 2.0 96 3.0 97 2.0 98 0.5 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09019334099342255 2 0.1008452331318503 3 0.09806329566614408 4 0.0995640777200119 5 0.09656251361227625 6 0.07899238224992121 7 0.09151110084559919 8 0.08755782128906929 9 0.08195734191731863 10-11 0.08878407004040033 12-13 0.08836311897651056 14-15 0.0929203717986214 16-17 0.09050447873629758 18-19 0.09297527845912876 20-21 0.10300489511180642 22-23 0.11160693859129277 24-25 0.13530831371030294 26-27 0.14112841972408305 28-29 0.1406891664400242 30-31 0.14262920177795088 32-33 0.13816346005668562 34-35 0.13298393174882472 36-37 0.1370470246263693 38-39 0.12859139890823598 40-41 0.1252054881769488 42-43 0.12489435043407375 44-45 0.11654853803695511 46-47 0.13550963813216327 48-49 0.15392167162229783 50-51 0.1653422570078286 52-53 0.17312070057970452 54-55 0.16872816773911575 56-57 0.13805364673567092 58-59 0.12893914109144922 60-61 0.1254800214794856 62-63 0.12227713294988962 64-65 0.1086968855844027 66-67 0.12218562184904402 68-69 0.11980633322705844 70-71 0.12818875006451533 72-73 0.15646568022580548 74-75 0.1709793408199175 76-77 0.17557319808236657 78-79 0.17156501186532933 80-81 0.17819041556655071 82-83 0.1782819266673963 84-85 0.15344581389790068 86-87 0.1553126403551509 88-89 0.17493262037644738 90-91 0.26331404157312704 92-93 0.5348823844425272 94-95 0.17520715367898418 96-97 0.18862268106294902 98-99 0.20756547893798805 100-101 0.21439220706106973 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2731909.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.337711851293925 #Duplication Level Percentage of deduplicated Percentage of total 1 76.48569317569563 43.85514636054514 2 13.41001399131638 15.377990363118373 3 4.718961167984595 8.117233070620387 4 2.130953119585036 4.887359037575307 5 1.0781735560534544 3.0910002341338934 6 0.6616643174910065 2.2762990787149437 7 0.39352053298067374 1.5794496849329436 8 0.24898690307937343 1.142107144280893 9 0.17115808312954467 0.883243156635148 >10 0.6433717119011739 6.100230081715491 >50 0.03244576114433731 1.3152877173588258 >100 0.019848060871077013 2.052922719118364 >500 0.0019536070378928802 0.8117006182382206 >1k 0.0024094486800418594 3.067129839645076 >5k 5.209618767658075E-4 2.2877643212516245 >10k+ 3.256011729786297E-4 3.1551365721154143 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 29060 1.063725036229245 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 22686 0.8304083335133052 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11310 0.4139962202254907 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11134 0.40755383872596046 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 10311 0.37742838432758924 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9527 0.34873050310240933 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 9179 0.33599215786470193 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8675 0.31754351993422913 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7622 0.27899904425806277 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7557 0.2766197556360772 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7377 0.2700309563751941 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5814 0.21281821612652543 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5520 0.202056510667083 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4646 0.17006422981146152 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4543 0.1662939724566228 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4403 0.16116935080926928 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4387 0.16058367976385743 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4069 0.1489434677362972 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3530 0.12921367439398604 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3468 0.1269441990930152 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3381 0.12375961278358832 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3345 0.1224418529314117 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2745 0.1004791887284679 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.660444033823967E-5 0.0 0.0 0.0 0.0 6 3.660444033823967E-5 0.0 0.0 0.0 0.0 7 3.660444033823967E-5 0.0 0.0 0.0 0.0 8 3.660444033823967E-5 0.0 0.0 0.0 0.0 9 3.660444033823967E-5 0.0 0.0 0.0 0.0 10-11 3.660444033823967E-5 0.0 0.0 0.0 0.0 12-13 3.660444033823967E-5 0.0 0.0 7.320888067647934E-5 0.0 14-15 3.660444033823967E-5 0.0 0.0 1.6471998152207853E-4 0.0 16-17 3.660444033823967E-5 0.0 0.0 2.1962664202943802E-4 0.0 18-19 3.660444033823967E-5 0.0 0.0 3.4774218321327686E-4 0.0 20-21 3.660444033823967E-5 0.0 0.0 6.588799260883141E-4 0.0 22-23 3.660444033823967E-5 0.0 0.0 0.0014641776135295868 0.0 24-25 3.660444033823967E-5 0.0 0.0 0.002525706383338537 0.0 26-27 3.660444033823967E-5 0.0 0.0 0.003239492969934211 0.0 28-29 3.660444033823967E-5 0.0 0.0 0.005893314894456587 0.0 30-31 3.660444033823967E-5 0.0 0.0 0.014312336172251711 0.0 32-33 3.660444033823967E-5 0.0 0.0 0.03144321425054788 0.0 34-35 3.660444033823967E-5 0.0 0.0 0.061294135346382325 0.0 36-37 3.660444033823967E-5 0.0 0.0 0.10294998845129907 0.0 38-39 3.660444033823967E-5 0.0 0.0 0.15628265802411428 0.0 40-41 3.660444033823967E-5 0.0 0.0 0.21622242907798173 0.0 42-43 3.660444033823967E-5 0.0 0.0 0.2803900129909159 0.0 44-45 3.660444033823967E-5 0.0 0.0 0.3548434446388954 0.0 46-47 3.660444033823967E-5 0.0 0.0 0.4444511145869061 0.0 48-49 3.660444033823967E-5 0.0 0.0 0.546156552066705 0.0 50-51 3.660444033823967E-5 0.0 0.0 0.649856931544938 0.0 52-53 3.660444033823967E-5 0.0 0.0 0.7475907872480378 0.0 54-55 3.660444033823967E-5 0.0 0.0 0.8541097086323153 0.0 56-57 3.660444033823967E-5 0.0 0.0 0.9779608325167493 0.0 58-59 3.660444033823967E-5 0.0 0.0 1.1305464420667013 0.0 60-61 3.660444033823967E-5 0.0 0.0 1.3294915753050338 0.0 62-63 3.660444033823967E-5 0.0 0.0 1.5119830126113278 0.0 64-65 3.660444033823967E-5 0.0 0.0 1.674836167676156 0.0 66-67 3.660444033823967E-5 0.0 3.660444033823967E-5 1.859908218026296 0.0 68-69 3.660444033823967E-5 0.0 3.660444033823967E-5 2.067601812505468 0.0 70-71 3.660444033823967E-5 1.8302220169119835E-5 3.660444033823967E-5 2.3102160430673204 0.0 72-73 3.660444033823967E-5 3.660444033823967E-5 3.660444033823967E-5 2.5741706623463667 0.0 74-75 3.660444033823967E-5 3.660444033823967E-5 3.660444033823967E-5 2.8434146232542883 0.0 76-77 3.660444033823967E-5 3.660444033823967E-5 3.660444033823967E-5 3.1060880871214964 0.0 78-79 3.660444033823967E-5 3.660444033823967E-5 3.660444033823967E-5 3.415304096878776 0.0 80-81 3.660444033823967E-5 3.660444033823967E-5 3.660444033823967E-5 3.7592211160767066 0.0 82-83 3.660444033823967E-5 3.660444033823967E-5 3.660444033823967E-5 4.100337895588762 0.0 84-85 3.660444033823967E-5 3.660444033823967E-5 3.660444033823967E-5 4.499124970853714 0.0 86-87 7.320888067647934E-5 3.660444033823967E-5 3.660444033823967E-5 4.93424195315437 0.0 88-89 9.151110084559918E-5 3.660444033823967E-5 3.660444033823967E-5 5.336652868012807 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13635 0.0 23.737516 1 TACATGG 13660 0.0 23.589844 2 ACATGGG 13900 0.0 22.567982 3 GAGTACT 8555 0.0 21.605366 12-13 AGTACTT 8990 0.0 20.243235 12-13 GTATAGC 1480 0.0 20.201557 1 CATGGGA 10770 0.0 20.18161 4 CATGGGG 3220 0.0 19.896832 4 TAGCCCT 2245 0.0 19.8709 4 TATCGCG 75 7.426937E-4 19.111895 90-91 GTACTTT 9780 0.0 18.996216 14-15 GTGTAGC 1870 0.0 18.780024 1 TCCGACG 115 1.9889612E-6 18.655989 92-93 GCTTATA 3225 0.0 18.541584 1 GTAGCCC 1570 0.0 17.532118 3 ATGGGAG 3665 0.0 17.222004 5 ATGGGAT 3790 0.0 17.154867 5 ATAGCCC 1205 0.0 16.935087 3 GTCGGGA 425 5.820766E-11 16.749752 2 ACTTTTT 12065 0.0 16.304043 16-17 >>END_MODULE