FastQCFastQC Report
Fri 27 May 2016
SRR939611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939611_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731909
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA296831.0865296025599682No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA255290.9344747573949206No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT160220.586476343099276No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA119670.43804533752771413No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG118940.43537321338302265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118300.4330305292013753No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT106060.38822669422736994No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT97910.3583940753517046No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC94070.34433797026182056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85990.3147615824685229No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG78390.28694220781146074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69110.25297328717757434No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG64700.23683072898841068No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG54260.19861569327528847No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT52220.19114838744628757No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG50860.18617018356028694No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50180.18368108161728666No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA48860.17884929549263903No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT43600.15959535987472498No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41890.15333600057688598No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT40620.14868723665392955No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37040.13558284701283974No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA33890.12405244830629425No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27490.10062560648982086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG131750.021.8801782
GTACATG133200.021.8225171
ACATGGG131700.020.922093
GTATAGC13250.020.5888791
ACCGACG600.004113965720.30105266-67
TAGCCCT20900.020.248344
AGTACTT96000.019.78771612-13
GAGTACT92100.019.40186312-13
GCGTTTA18050.019.27718262-63
CATGGGA102800.018.3419084
GTAGCCC13850.018.3331573
GCTTATA32900.018.2993071
GTACTTT101450.017.63686214-15
GTAGGAC9600.017.1431273
ATAGCCC12200.016.9584473
CGCGTCG1751.8815626E-816.66925672-73
CATGGGG30300.016.4496444
ACTGCGC2401.4551915E-1116.2215962-63
TACTTTT118550.016.00398814-15
CGTTGAG14600.015.99937562-63
Produced by FastQC (version 0.11.5)