FastQCFastQC Report
Fri 27 May 2016
SRR939610_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939610_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2675635
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA121030.4523412199347071No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA95180.3557286401172058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45070.16844599506285424No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA43050.16089638534403983No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT42740.15973778187234058No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT39350.14706789229472628No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG39160.14635778048949127No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36780.1374626957712842No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC32080.11989677216810216No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG31890.11918666036286714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30150.1126835311991359No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118900.029.0248261
TACATGG135800.023.5170462
ACATGGG135250.022.9145093
GAGTACT84650.022.136512-13
CATGGGG31650.020.7457054
GTATAGC12600.019.6221851
GTACTTT95750.019.62123314-15
AGTACTT90700.019.59309812-13
CATGGGA106250.019.3840524
GCTTATA28750.018.9908621
TAGCCCT20500.018.434154
GTACCGT1801.2956049E-418.3553566
CTTTGCG4650.018.2875462
ATGGGAT39500.017.565385
GTAGCCC13000.017.4438323
GTATTAG8903.274181E-1117.3623261
ACTTTTT121950.016.3946616-17
ATGGGAG34550.015.9836195
TACTTTT116800.015.29695614-15
GTGTAGC15500.015.2863151
Produced by FastQC (version 0.11.5)