##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939610_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2675635 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.970865607603429 2.0 2.0 31.0 2.0 33.0 2 22.426520433467196 16.0 16.0 31.0 16.0 34.0 3 27.051138514782473 28.0 25.0 31.0 16.0 34.0 4 32.268682013802334 35.0 32.0 35.0 25.0 37.0 5 32.88485798698253 35.0 33.0 35.0 26.0 37.0 6 33.296674994907754 35.0 33.0 37.0 28.0 37.0 7 33.49314200180518 35.0 33.0 37.0 28.0 37.0 8 33.42444541202369 35.0 33.0 37.0 27.0 37.0 9 34.77988103758547 37.0 34.0 39.0 27.0 39.0 10-11 34.624478114541034 37.0 34.0 39.0 27.0 39.0 12-13 34.643136675966645 37.0 34.0 39.0 27.0 39.0 14-15 35.275383226785415 38.0 33.5 40.0 27.0 41.0 16-17 34.920423936747724 38.0 33.0 40.0 25.0 41.0 18-19 35.29416904772138 38.0 34.0 40.0 27.0 41.0 20-21 35.314758552642644 38.0 34.0 40.0 26.5 41.0 22-23 35.17053596622858 38.0 34.0 40.0 26.0 41.0 24-25 34.98359847288587 38.0 34.0 40.0 25.0 41.0 26-27 35.01252973593184 38.0 34.0 40.0 25.0 41.0 28-29 34.75621058178713 38.0 33.5 40.0 24.0 41.0 30-31 34.67066602881185 38.0 33.0 40.0 24.0 41.0 32-33 34.38760910961323 38.0 33.0 40.0 24.0 41.0 34-35 34.14403010874054 38.0 33.0 40.0 24.0 41.0 36-37 33.839316087582944 38.0 33.0 40.0 21.5 41.0 38-39 33.60513055779282 38.0 32.0 40.0 19.5 41.0 40-41 33.220689294317054 37.0 31.0 40.0 17.0 41.0 42-43 33.028461281153824 37.0 31.0 40.0 16.5 41.0 44-45 32.74701482078086 37.0 31.0 40.0 15.0 41.0 46-47 32.40834138438165 37.0 30.0 40.0 14.0 41.0 48-49 32.04786545997492 37.0 30.0 39.0 9.0 40.5 50-51 31.42317804932287 35.5 30.0 38.5 9.0 40.0 52-53 31.450137630880146 36.0 30.0 39.0 8.5 40.0 54-55 31.615766350791496 36.0 30.0 39.0 8.0 40.0 56-57 31.398858028094267 36.0 29.5 39.0 7.5 40.0 58-59 31.037082412212428 36.0 29.0 39.0 4.0 40.0 60-61 30.435831120462993 35.0 28.0 39.0 2.0 40.0 62-63 30.008555726023914 35.0 28.0 38.0 2.0 40.0 64-65 29.376647225798735 34.0 26.5 38.0 2.0 40.0 66-67 28.792677065444277 34.0 26.0 38.0 2.0 39.5 68-69 28.503979989796814 34.0 26.0 37.0 2.0 39.0 70-71 27.947990663898477 33.5 25.5 37.0 2.0 39.0 72-73 27.32828543504626 32.5 24.5 36.0 2.0 39.0 74-75 26.748256208339328 32.0 22.5 36.0 2.0 38.0 76-77 26.04648503999985 31.5 21.0 35.0 2.0 37.5 78-79 25.35402941731589 31.0 19.0 35.0 2.0 37.0 80-81 24.61319200862599 31.0 16.5 34.0 2.0 36.0 82-83 23.912127962147302 30.0 10.0 34.0 2.0 36.0 84-85 23.200136416215216 29.5 6.5 34.0 2.0 35.0 86-87 22.433270046175956 29.0 2.0 34.0 2.0 35.0 88-89 21.525522352637786 29.0 2.0 33.5 2.0 35.0 90-91 20.516060673447612 26.5 2.0 33.0 2.0 34.0 92-93 19.50647752776444 26.0 2.0 32.0 2.0 34.0 94-95 18.53029168776758 25.0 2.0 32.0 2.0 34.0 96-97 17.53012350339265 22.5 2.0 31.5 2.0 34.0 98-99 16.40995053510662 17.5 2.0 31.0 2.0 34.0 100-101 14.81264260633457 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.940829150333265 1101 2 8.138983824251877 1101 3 3.8455191981691463 1101 4 2.4970870315192997 1101 5 1.6862614805552951 1101 6 1.5013825461747814 1101 7 1.1531853336937488 1101 8 1.204894720415595 1101 9 1.3427141987393938 1101 10-11 1.2074125944626672 1101 12-13 0.8737694925709647 1101 14-15 1.1152390971606891 1101 16-17 1.20101034149463 1101 18-19 1.241384323590701 1101 20-21 1.220196551973281 1101 22-23 1.1202023542099013 1101 24-25 1.3801782407631933 1101 26-27 1.4520677306552443 1101 28-29 1.4692844892963919 1101 30-31 1.4063528653250614 1101 32-33 1.3664101311873864 1101 34-35 1.531814506880842 1101 36-37 1.3444661653597905 1101 38-39 1.2844309464773787 1101 40-41 1.4014038789839134 1101 42-43 1.39292437439952 1101 44-45 1.1930011852000604 1101 46-47 0.9686119059186211 1101 48-49 0.9769510826875276 1101 50-51 0.9172557482609811 1101 52-53 1.0342514222075856 1101 54-55 0.9424515217627167 1101 56-57 0.7943433527142716 1101 58-59 1.0929623388098975 1101 60-61 1.0419814192724104 1101 62-63 0.8172119703675982 1101 64-65 0.7241264119182169 1101 66-67 0.7355957378671611 1101 68-69 0.7255850140836522 1101 70-71 0.476994778837998 1101 72-73 0.43234476864453697 1101 74-75 0.4120192908172875 1101 76-77 0.34445831367813895 1101 78-79 0.5692911889748018 1101 80-81 0.41283645421929194 1101 82-83 0.41479738816105893 1101 84-85 0.3367478907510666 1101 86-87 0.3977893614813617 1101 88-89 0.26044386468136693 1101 90-91 0.5318350988891858 1101 92-93 0.3679828017426203 1101 94-95 0.41196920668382475 1101 96-97 -0.11483181739935588 1101 98-99 -0.650132999548056 1101 100-101 -0.8170625870666033 1102 1 14.95048083935378 1102 2 8.203043940028031 1102 3 3.8578890903533534 1102 4 2.2479301883143847 1102 5 1.649004176371193 1102 6 1.453905850787983 1102 7 1.261089853455296 1102 8 1.1317852062784368 1102 9 1.2965358244579832 1102 10-11 1.282560447671166 1102 12-13 1.034402892808771 1102 14-15 1.1092502777606725 1102 16-17 1.2436292356258178 1102 18-19 1.2630362091138707 1102 20-21 1.1384503201121845 1102 22-23 1.1466241912690975 1102 24-25 1.101804786467099 1102 26-27 1.2671888103771707 1102 28-29 1.259274784471458 1102 30-31 1.1842855671763957 1102 32-33 1.1971462792541345 1102 34-35 1.282764938083865 1102 36-37 1.2271367868094671 1102 38-39 1.2467629667853188 1102 40-41 1.2233469092553548 1102 42-43 1.1001809116217416 1102 44-45 1.1465367040572971 1102 46-47 1.0574710826928069 1102 48-49 0.9997287228911631 1102 50-51 0.8162008748949141 1102 52-53 0.9420793961649743 1102 54-55 1.06754664007536 1102 56-57 0.9817080064986499 1102 58-59 0.9245675850297346 1102 60-61 0.9782654337815266 1102 62-63 0.676034087342817 1102 64-65 0.6539077926902301 1102 66-67 0.801989072015914 1102 68-69 0.43944619598695667 1102 70-71 0.6914448420998234 1102 72-73 0.8850759800833963 1102 74-75 0.7142313492627892 1102 76-77 0.6729901982388498 1102 78-79 0.6784893929806515 1102 80-81 0.6623931953631406 1102 82-83 0.45134337278974 1102 84-85 0.4410658611192666 1102 86-87 0.538367836219745 1102 88-89 0.5073782728975829 1102 90-91 0.580540437144446 1102 92-93 0.7274050789276565 1102 94-95 0.7332000401558467 1102 96-97 -0.18146388723399198 1102 98-99 -0.25828440083254023 1102 100-101 -0.29769579681714653 1103 1 14.879779885408926 1103 2 8.004838996500652 1103 3 3.8985071067577586 1103 4 2.3809918355822077 1103 5 1.7967321961698488 1103 6 1.2956907196418683 1103 7 1.1228615933320825 1103 8 1.0582948316789142 1103 9 1.1596508982865643 1103 10-11 1.0511545562861855 1103 12-13 0.7979718562185667 1103 14-15 0.9762631384118734 1103 16-17 1.1992231246129137 1103 18-19 1.1972912492337073 1103 20-21 1.0431825712788623 1103 22-23 1.1694216665706918 1103 24-25 1.4095719204883395 1103 26-27 1.3784480039482574 1103 28-29 1.38795957847654 1103 30-31 1.2885685307215695 1103 32-33 1.2524047365116715 1103 34-35 1.1967927826184663 1103 36-37 1.3738579757415792 1103 38-39 1.3260648373236776 1103 40-41 1.1500504743862336 1103 42-43 1.261415196172841 1103 44-45 1.198440600529274 1103 46-47 1.0992380063387515 1103 48-49 1.0099326554483525 1103 50-51 0.8680432901592035 1103 52-53 1.2356228223507522 1103 54-55 1.0098098205505828 1103 56-57 1.2071774916099685 1103 58-59 1.119710399179226 1103 60-61 1.2543228740303114 1103 62-63 1.182715207571814 1103 64-65 1.042576282609506 1103 66-67 1.0200355230771159 1103 68-69 1.0023012956157338 1103 70-71 0.963639192841228 1103 72-73 1.0214786744455822 1103 74-75 0.9032373649250367 1103 76-77 0.9119675307871695 1103 78-79 0.8870420498697342 1103 80-81 0.8327038105988898 1103 82-83 0.8525083747157431 1103 84-85 0.8425973153921014 1103 86-87 0.7791362807037991 1103 88-89 0.5889141635569679 1103 90-91 0.5458868283558225 1103 92-93 0.973667873714831 1103 94-95 1.1186728464698312 1103 96-97 0.273742731017137 1103 98-99 0.03241835006800642 1103 100-101 -0.24887168274308635 1104 1 14.926692076602869 1104 2 8.160396534387562 1104 3 4.07257706163254 1104 4 2.4281172073292865 1104 5 1.7955646189896157 1104 6 1.3923264955111705 1104 7 1.1489302897461897 1104 8 1.13141264402919 1104 9 1.268754978835723 1104 10-11 1.0178149402413865 1104 12-13 0.9356752230325185 1104 14-15 1.085365420474922 1104 16-17 1.4256283057876118 1104 18-19 1.1388579560425853 1104 20-21 1.2563608463340472 1104 22-23 1.228698569644763 1104 24-25 1.4149293708009694 1104 26-27 1.2319414073410186 1104 28-29 1.2678672274139657 1104 30-31 1.3091392551086116 1104 32-33 1.4047996965301266 1104 34-35 1.433098869606681 1104 36-37 1.3598421337167608 1104 38-39 1.253099056998927 1104 40-41 1.393428448542707 1104 42-43 1.2383408592267031 1104 44-45 1.0007225916404394 1104 46-47 0.9331533297760899 1104 48-49 0.9132010802191175 1104 50-51 0.9518785210152707 1104 52-53 0.9847294543521947 1104 54-55 1.0552024108837479 1104 56-57 1.1714902517010302 1104 58-59 1.1783791741512033 1104 60-61 1.0060290378498564 1104 62-63 0.717920453204254 1104 64-65 0.8932139298635526 1104 66-67 0.8690394255521703 1104 68-69 1.0408935701826927 1104 70-71 1.0888132263467831 1104 72-73 0.9871379011349255 1104 74-75 0.8026820883346595 1104 76-77 0.7043504145709143 1104 78-79 0.6555469132159644 1104 80-81 0.6445348037421006 1104 82-83 0.6861323434038624 1104 84-85 0.3885172218516395 1104 86-87 0.4162670095414889 1104 88-89 0.42822386622165354 1104 90-91 0.6368775160345628 1104 92-93 0.777314795881626 1104 94-95 0.8865716199982252 1104 96-97 0.435171366848639 1104 98-99 0.12289898708698743 1104 100-101 -0.10821220426568878 1105 1 14.872693750903167 1105 2 8.079359971694199 1105 3 3.9428198589653647 1105 4 2.346380045832099 1105 5 1.719966439642505 1105 6 1.2932504057231924 1105 7 0.9351178832237963 1105 8 1.0434558542038346 1105 9 0.9617523451075911 1105 10-11 1.0337068762658888 1105 12-13 0.8451530402188112 1105 14-15 0.963560846861256 1105 16-17 1.113822466576103 1105 18-19 1.020283154809242 1105 20-21 1.0733035973012761 1105 22-23 0.9692699846902073 1105 24-25 1.1442492761095053 1105 26-27 1.1731281003885812 1105 28-29 1.3650580046314005 1105 30-31 1.317336142166134 1105 32-33 0.9967493048014262 1105 34-35 1.1611892775407426 1105 36-37 1.2645330411813376 1105 38-39 1.3413247863650994 1105 40-41 1.5697827869145584 1105 42-43 1.4979761743546263 1105 44-45 1.2411647705907356 1105 46-47 1.1601201033128206 1105 48-49 1.1301808713336214 1105 50-51 1.130595269037837 1105 52-53 1.3145575733193517 1105 54-55 1.0873626317638774 1105 56-57 1.0821345837377692 1105 58-59 1.245800271143814 1105 60-61 1.2545679503444696 1105 62-63 1.1474670361257715 1105 64-65 1.140419623055518 1105 66-67 1.1988857434356142 1105 68-69 1.0277633494429246 1105 70-71 1.108140475453311 1105 72-73 1.1489984084636156 1105 74-75 1.0004849795596158 1105 76-77 1.0952592113561082 1105 78-79 1.124043697003561 1105 80-81 0.8921985029941872 1105 82-83 1.0629238958713394 1105 84-85 1.0006545430234972 1105 86-87 0.8496763421836597 1105 88-89 0.8014870889238175 1105 90-91 0.9725260171694359 1105 92-93 1.157043748597033 1105 94-95 1.150142242617644 1105 96-97 0.5436674773952817 1105 98-99 0.1008416031681243 1105 100-101 0.2705321038150803 1106 1 14.670464844152342 1106 2 7.913326024484821 1106 3 3.574880273191738 1106 4 2.2909073917717038 1106 5 1.623098663001052 1106 6 1.188455292718686 1106 7 0.884219581203638 1106 8 0.868662935230283 1106 9 1.0932859298611461 1106 10-11 0.9713453580917673 1106 12-13 0.6317089666870928 1106 14-15 0.8452707759590368 1106 16-17 1.0689198127038964 1106 18-19 0.9057630770438365 1106 20-21 0.6937474990744334 1106 22-23 0.625333597770819 1106 24-25 0.7670245442112744 1106 26-27 0.7482610346036225 1106 28-29 0.8128808041148403 1106 30-31 0.7324301817630428 1106 32-33 0.7476231651738701 1106 34-35 0.9507394249648584 1106 36-37 0.9380380091963687 1106 38-39 0.9598526630648436 1106 40-41 0.8496806274973636 1106 42-43 0.8361085632779321 1106 44-45 0.8507049221908929 1106 46-47 0.6514480828294822 1106 48-49 0.9158140422229053 1106 50-51 1.003739782304816 1106 52-53 0.9950847505972895 1106 54-55 0.9435511092660072 1106 56-57 0.9522042972249594 1106 58-59 1.1026563081712482 1106 60-61 1.1136942495812292 1106 62-63 1.023171371278412 1106 64-65 0.9894264607613046 1106 66-67 1.0151235616207224 1106 68-69 0.9704551300217226 1106 70-71 1.0561675508910504 1106 72-73 1.016823170901997 1106 74-75 0.9106627580044169 1106 76-77 0.8638294440496423 1106 78-79 0.8041625605955822 1106 80-81 0.8671660630197486 1106 82-83 0.9580274786690843 1106 84-85 0.756456992572101 1106 86-87 0.7745380479581563 1106 88-89 0.829526435212177 1106 90-91 1.12255693847424 1106 92-93 1.6530083158309807 1106 94-95 1.5256852414553599 1106 96-97 1.0325318174362508 1106 98-99 0.8084682786776156 1106 100-101 1.1230430781387248 1107 1 14.736053963553118 1107 2 7.918845049988249 1107 3 3.6797712769909126 1107 4 2.217535788179788 1107 5 1.4493746378439951 1107 6 1.154791462993245 1107 7 0.9754743652835387 1107 8 0.7857463825340432 1107 9 0.6117587808549558 1107 10-11 0.5452807433451454 1107 12-13 0.4127205289425575 1107 14-15 0.620473833770788 1107 16-17 0.7127338905694529 1107 18-19 0.5510667454514149 1107 20-21 0.4774404909464067 1107 22-23 0.6557290055302403 1107 24-25 0.8547030373781652 1107 26-27 0.5203066056353549 1107 28-29 0.7246926875586226 1107 30-31 0.792115394840728 1107 32-33 0.8061887303684117 1107 34-35 0.8263264470716649 1107 36-37 0.7128375581234252 1107 38-39 0.7304201893839846 1107 40-41 1.0190024778486588 1107 42-43 0.9649700778384158 1107 44-45 0.6816251771704245 1107 46-47 0.7121162983036982 1107 48-49 0.5917296324492725 1107 50-51 0.7005316605160736 1107 52-53 0.6413376766318528 1107 54-55 0.7063582908195336 1107 56-57 0.6629983503372046 1107 58-59 0.7367756387226265 1107 60-61 0.48016161380644107 1107 62-63 0.3429076454892446 1107 64-65 0.3028355014076247 1107 66-67 0.2840580077339574 1107 68-69 0.36144446715065826 1107 70-71 0.39281275183728326 1107 72-73 0.3597304964570114 1107 74-75 0.33142337344235884 1107 76-77 0.0051015944853745054 1107 78-79 0.34497123525100903 1107 80-81 0.06912571165516468 1107 82-83 0.20694609866393776 1107 84-85 0.11867144457013623 1107 86-87 0.13667132206257193 1107 88-89 0.4248110113409709 1107 90-91 0.6988382696298139 1107 92-93 0.9602899133738418 1107 94-95 0.8255983382586862 1107 96-97 0.3306484069839186 1107 98-99 0.2551721348180074 1107 100-101 0.1684809162262031 1108 1 14.329286660922527 1108 2 7.408225524139755 1108 3 3.2194573522117516 1108 4 -0.10290946468101936 1108 5 0.2203154935738567 1108 6 0.45842596214587417 1108 7 0.46512667230094706 1108 8 0.4511174297092282 1108 9 0.547235852221398 1108 10-11 0.5753125337301341 1108 12-13 0.3040988692060722 1108 14-15 0.453092980927984 1108 16-17 0.6980579036286798 1108 18-19 0.29938583411767894 1108 20-21 0.370580120710045 1108 22-23 -0.32614425333338914 1108 24-25 0.1677642785107949 1108 26-27 0.16125868384447983 1108 28-29 0.3240091685040696 1108 30-31 0.36329360050009285 1108 32-33 0.646044490225627 1108 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0.13703832602120514 2312 12-13 -0.6243724471203151 2312 14-15 0.11151876006751849 2312 16-17 -0.2011188147792069 2312 18-19 0.030404527068448317 2312 20-21 0.10959359125772039 2312 22-23 0.28887379892440634 2312 24-25 0.33186832136346567 2312 26-27 0.3140287479315518 2312 28-29 -0.5102637293831478 2312 30-31 0.3293527566336891 2312 32-33 0.3907074555055843 2312 34-35 -0.48327485429496875 2312 36-37 0.18248626764212617 2312 38-39 0.328244228750755 2312 40-41 0.31787783709276596 2312 42-43 0.3397465337598149 2312 44-45 0.18247440214617683 2312 46-47 0.24312403625081913 2312 48-49 0.3308524237142123 2312 50-51 0.25879040019559696 2312 52-53 0.2521014974482547 2312 54-55 0.12732582862095754 2312 56-57 0.3039746031710564 2312 58-59 0.1657401155465763 2312 60-61 0.2740023667238809 2312 62-63 0.052757188790408804 2312 64-65 -0.020008001832369615 2312 66-67 -0.06399563878405701 2312 68-69 0.10485478139041149 2312 70-71 0.17912700376917812 2312 72-73 0.07033327078369567 2312 74-75 0.17688377328705585 2312 76-77 0.16539067953330644 2312 78-79 0.35769285888659397 2312 80-81 0.333471956757613 2312 82-83 0.3958668175875637 2312 84-85 0.25696432245071676 2312 86-87 0.4267283228415799 2312 88-89 0.8302778386066798 2312 90-91 0.9728946242826773 2312 92-93 1.229691181847599 2312 94-95 1.4503715793415708 2312 96-97 1.0758277284206201 2312 98-99 0.8161911799726163 2312 100-101 0.974216007666362 2313 1 -14.186678012990516 2313 2 -7.37910962197688 2313 3 -3.644425935519589 2313 4 -1.0674574074187113 2313 5 -0.4662568967673195 2313 6 -1.136823641883744 2313 7 -0.4998975050795096 2313 8 -0.3265742993257419 2313 9 -2.7551333482395037 2313 10-11 -0.668476238369287 2313 12-13 -0.9016926323242984 2313 14-15 -1.0484817593725282 2313 16-17 -0.8483316647814547 2313 18-19 -0.2327125145788287 2313 20-21 -1.2978929564256987 2313 22-23 -2.078366180014662 2313 24-25 -1.4644120157058964 2313 26-27 -0.4068566376228162 2313 28-29 0.0344281836436906 2313 30-31 -1.3704291966025366 2313 32-33 -0.5137964438363838 2313 34-35 -1.3662314361905956 2313 36-37 -1.4344792344557007 2313 38-39 -0.8465426237495208 2313 40-41 -0.637440284517595 2313 42-43 -0.3894050317616333 2313 44-45 -0.5041243988917614 2313 46-47 -1.174874089042838 2313 48-49 -0.5159499505990084 2313 50-51 -1.492995174954391 2313 52-53 -0.6955625570646156 2313 54-55 -1.3730472237740194 2313 56-57 -0.9217037973313857 2313 58-59 -0.8177641214314164 2313 60-61 -2.227643313777996 2313 62-63 -0.8311432392754057 2313 64-65 -0.5610006753666603 2313 66-67 -1.7329883737067213 2313 68-69 -0.7984207603029212 2313 70-71 -0.4068350109970851 2313 72-73 -1.4647448695265055 2313 74-75 -0.9073652698807742 2313 76-77 -0.18732032527623232 2313 78-79 -0.335532934617099 2313 80-81 -0.42486800730658203 2313 82-83 -0.1139624046926393 2313 84-85 0.08362954098150155 2313 86-87 0.007006010766726689 2313 88-89 0.31074335132941755 2313 90-91 0.6786111800174162 2313 92-93 0.9556316634629596 2313 94-95 1.0246225822150201 2313 96-97 0.6169620674213583 2313 98-99 0.49461116141443995 2313 100-101 0.6840603409296495 2314 1 -14.186678012990516 2314 2 -7.248019041077635 2314 3 -3.448766379672307 2314 4 -1.3903714212027616 2314 5 -1.3115169339623733 2314 6 -6.075987081332382 2314 7 -1.8561613711773113 2314 8 -4.060756664493667 2314 9 -1.4585857057496199 2314 10-11 -0.41047279235682055 2314 12-13 -2.820438721373641 2314 14-15 -3.058897961101003 2314 16-17 -2.7829017884003093 2314 18-19 -1.3672355426939191 2314 20-21 -2.096935730739233 2314 22-23 -1.2138793076812178 2314 24-25 -3.102190431634135 2314 26-27 -4.854461877749721 2314 28-29 -1.9089690923654885 2314 30-31 -2.0536371060216467 2314 32-33 -4.105636669194659 2314 34-35 -2.6999834873884936 2314 36-37 -0.9994029845924501 2314 38-39 -1.5252218710966403 2314 40-41 -2.4747752041106352 2314 42-43 -1.3738607258870061 2314 44-45 -2.5611425588305003 2314 46-47 -2.0242285907384954 2314 48-49 -1.2222232248578422 2314 50-51 -2.304771949924305 2314 52-53 -0.8806826752136168 2314 54-55 -0.23809623372916278 2314 56-57 -1.0600023970469508 2314 58-59 -1.2829044233677465 2314 60-61 -0.40008352819655 2314 62-63 -2.873703024856855 2314 64-65 -1.2140980388936278 2314 66-67 -2.3652504894489823 2314 68-69 -2.291456533194289 2314 70-71 -2.8557282393090873 2314 72-73 -2.6738400451090456 2314 74-75 -2.1146464207395432 2314 76-77 -1.002373109435517 2314 78-79 -0.5716562799841256 2314 80-81 -0.3759792095820451 2314 82-83 -0.5363931562515454 2314 84-85 -0.21751333178952947 2314 86-87 -0.05960032333238985 2314 88-89 0.10434596592300949 2314 90-91 0.4529692918213719 2314 92-93 0.8075262592402304 2314 94-95 0.9229195409722664 2314 96-97 0.7546915827916649 2314 98-99 0.6452805630116387 2314 100-101 0.757002360402355 2315 1 -14.186678012990516 2315 2 -7.4436553126075236 2315 3 -4.853698497200167 2315 4 -3.19114919898054 2315 5 -3.6256128475050957 2315 6 -2.9345642512074264 2315 7 -3.353369071278628 2315 8 -3.9123672690191675 2315 9 -2.690798471707069 2315 10-11 -5.01368099904375 2315 12-13 -4.552313763589112 2315 14-15 -3.4133375085511943 2315 16-17 -4.38691546621186 2315 18-19 -1.5454945768040176 2315 20-21 -3.3562906783920496 2315 22-23 -4.386883191504246 2315 24-25 -5.145391208398742 2315 26-27 -4.814188490380587 2315 28-29 -2.706508943767048 2315 30-31 -3.313929743644067 2315 32-33 -4.486000904250382 2315 34-35 -4.073378455304741 2315 36-37 -5.39594874615949 2315 38-39 -3.9403022493472335 2315 40-41 -4.998027990331508 2315 42-43 -3.497434011939017 2315 44-45 -2.0416346223225617 2315 46-47 -1.5774405123865876 2315 48-49 -1.3203576389071472 2315 50-51 -1.2384154825146467 2315 52-53 -0.9161966907489081 2315 54-55 -2.5007868787815113 2315 56-57 -4.474241674318144 2315 58-59 -3.8663905766943287 2315 60-61 -4.88715005977372 2315 62-63 -2.804530785748444 2315 64-65 -3.414630629234729 2315 66-67 -2.1672876390606 2315 68-69 -1.70917352069851 2315 70-71 -2.501289536168258 2315 72-73 -2.7430332230894585 2315 74-75 -3.3266028543768265 2315 76-77 -3.0525765879900604 2315 78-79 -2.2862886676909824 2315 80-81 -1.9969662072179766 2315 82-83 -1.4785648887743434 2315 84-85 -1.3066092453322504 2315 86-87 -1.9166356154634272 2315 88-89 -2.593307360655846 2315 90-91 -2.0752500597490418 2315 92-93 -1.2580051490677775 2315 94-95 -1.2298945421077754 2315 96-97 -1.8083599200789848 2315 98-99 -1.337714708855973 2315 100-101 -0.921154681137665 2316 1 -14.186678012990516 2316 2 -7.444523256929237 2316 3 -5.296858566294002 2316 4 -3.0855468006294053 2316 5 -2.1030189013597855 2316 6 -1.3314862381620642 2316 7 -4.530050072695019 2316 8 -2.912494157467247 2316 9 -1.3237335416124623 2316 10-11 -4.509365709726129 2316 12-13 -3.806834449310685 2316 14-15 -2.191654846991959 2316 16-17 -3.3137311869388135 2316 18-19 -2.4602512818732336 2316 20-21 -3.3735237015093276 2316 22-23 -3.2855293919982493 2316 24-25 -3.7346426519826466 2316 26-27 -4.348120955883502 2316 28-29 -3.965042448183368 2316 30-31 -2.645702030136313 2316 32-33 -2.9170090694195068 2316 34-35 -4.270038293459322 2316 36-37 -2.8118301908881236 2316 38-39 -3.636726649061792 2316 40-41 -3.2879615447514468 2316 42-43 -7.175979612902182 2316 44-45 -3.781290394693343 2316 46-47 -5.080838764993693 2316 48-49 -4.766104056785892 2316 50-51 -3.673502416478552 2316 52-53 -5.016839504893213 2316 54-55 -5.177586396742093 2316 56-57 -5.177758158710809 2316 58-59 -4.001424659455438 2316 60-61 -3.688310622069512 2316 62-63 -2.7785703660597783 2316 64-65 -2.9917276060661955 2316 66-67 -3.297542788493388 2316 68-69 -3.203395824931217 2316 70-71 -3.3643696108661416 2316 72-73 -4.422664665682401 2316 74-75 -5.077331637153261 2316 76-77 -4.717886038047098 2316 78-79 -5.00363126593129 2316 80-81 -4.955522492325155 2316 82-83 -4.513908333317314 2316 84-85 -4.167489160907007 2316 86-87 -4.42943084609799 2316 88-89 -4.2576880419465795 2316 90-91 -3.5852502135187017 2316 92-93 -3.365465027999793 2316 94-95 -2.638726046436391 2316 96-97 -2.7649118461009685 2316 98-99 -2.872769465094038 2316 100-101 -2.7179256890636356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18723.0 3 5313.0 4 5522.0 5 6199.0 6 7465.0 7 9085.0 8 11303.0 9 14195.0 10 17353.0 11 20962.0 12 25122.0 13 27876.0 14 31002.0 15 34078.0 16 38193.0 17 40656.0 18 37822.0 19 37797.0 20 40864.0 21 44569.0 22 49761.0 23 55194.0 24 61455.0 25 68930.0 26 77165.0 27 86973.0 28 97990.0 29 111244.0 30 126110.0 31 144038.0 32 165476.0 33 192600.0 34 222453.0 35 260573.0 36 281008.0 37 176677.0 38 23761.0 39 128.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.7709154821075 19.37437993554998 13.861808268939022 21.992896313403502 2 13.900346020761244 22.19309828860002 41.75819695127654 22.1483587393622 3 19.99544979658795 27.58691786583486 30.412197538699182 22.005434798878014 4 11.344408794461371 16.867122926534073 41.18659021392986 30.6018780650747 5 12.046772158980138 39.88442421096866 35.269400555838494 12.799403074212709 6 27.868656104127687 38.49163230266918 19.543254843555072 14.096456749648063 7 25.731437660237994 34.824289880613925 22.63173439414363 16.812538065004453 8 23.345006035991354 34.85681131210286 21.911228815541975 19.88695383636381 9 26.007097873856665 18.21695300446967 22.05341296046914 33.72253616120452 10-11 22.94142841345129 26.895006295434676 29.65566058653729 20.50790470457674 12-13 26.657897406013024 24.57586233870156 28.65510306969036 20.11113718559506 14-15 23.075939810505126 26.010329278503864 28.77021846970519 22.14351244128582 16-17 20.22197004119317 29.283419954023536 29.72768940213384 20.766920602649446 18-19 19.69657099736334 29.889046266531516 31.069422256026808 19.344960480078335 20-21 21.578919484286978 28.245582406794302 29.57489001999943 20.60060808891929 22-23 20.444320697473543 28.295597892914472 29.093946857000656 22.16613455261132 24-25 21.0760187609263 28.004514549232447 30.24414601337808 20.675320676463173 26-27 21.899614391313225 27.085348929978032 30.78784780161847 20.227188877090274 28-29 20.47183841239538 27.98069617449927 31.308188319608693 20.239277093496653 30-31 20.48281323634413 29.073273100929857 30.489357685025837 19.954555977700174 32-33 20.2569255462805 28.744833114515227 30.03910632312536 20.959135016078918 34-35 21.248552003712266 27.70319793233808 29.911361841210844 21.136888222738815 36-37 21.64983236851142 27.555916794267315 30.793153640166548 20.00109719705472 38-39 22.001762570484566 27.008481270488666 31.23496202909421 19.754794129932563 40-41 20.97941350372603 27.73166969789222 31.496305799170727 19.792610999211018 42-43 20.055112881433168 28.912358386872654 30.416439432276288 20.616089299417887 44-45 20.70764644641346 29.037734153228524 29.273085766929064 20.981533633428953 46-47 20.71008355356437 28.459494033627454 28.481801205823466 22.348621206984713 48-49 21.352073274044756 28.06398461426369 29.19343446518065 21.39050764651091 50-51 21.987114225209776 28.74082246796586 29.155662369847402 20.116400936976966 52-53 20.870613025506177 29.003520878032894 29.818919580792357 20.30694651566857 54-55 19.91203720096498 28.352415482966386 30.39906057109499 21.33648674497364 56-57 21.161388010538456 29.473346866652108 28.42498916447927 20.940275958330165 58-59 21.015511350339338 28.673849754271004 28.876761057804824 21.433877837584838 60-61 20.93897580370039 27.847052483797167 29.7013668907682 21.51260482173424 62-63 22.457605220169736 27.623042417624067 29.158980143491753 20.760372218714444 64-65 21.776236279809964 28.219691544384375 29.654913524772404 20.349158651033257 66-67 20.52674674890972 28.325056786765064 29.858725426183945 21.28947103814127 68-69 20.41168265855371 29.510432951088227 29.32540135736017 20.752483032997894 70-71 20.79962805210641 29.018152308018696 28.4728696555194 21.709349984355494 72-73 20.758336532788956 28.24978228094654 28.400958900261266 22.59092228600324 74-75 20.909323557425342 28.906928539901095 28.65002244392204 21.53372545875152 76-77 21.687608542111995 27.65056324808582 29.13261855561946 21.529209654182722 78-79 20.87938376333509 27.8662979697378 29.675862796335533 21.578455470591575 80-81 20.613040529654427 29.403988894342636 28.891457561326533 21.0915130146764 82-83 20.73735636705593 28.86736502156133 28.60372106266836 21.791557548714373 84-85 21.036029726265767 28.090624510760826 28.42971173372505 22.443634029248358 86-87 21.35614520562056 28.233774868546117 28.94210410491963 21.467975820913693 88-89 22.099608109312854 27.24205898812641 29.09724672631556 21.561086176245176 90-91 20.90535625232565 27.780454685651012 29.69305282168666 21.621136240336682 92-93 20.535815107383808 28.861709373187633 29.241499753466776 21.360975765961783 94-95 20.640504859323443 29.13675702521018 28.21468735826544 22.008050757200937 96-97 20.26154862978928 28.41087263643125 28.021881545297934 23.30569718848153 98-99 21.114759690445535 28.176485031141002 27.920534691804377 22.78822058660909 100-101 21.770938034673804 28.300215926454936 28.175450505874394 21.753395532996862 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4473.0 1 3020.5 2 1492.0 3 1225.5 4 1016.5 5 1052.5 6 1496.5 7 3006.5 8 3858.5 9 3292.0 10 2738.0 11 2499.5 12 2553.5 13 2653.0 14 2754.0 15 2840.0 16 3122.5 17 3489.0 18 3886.0 19 4493.5 20 5364.5 21 6548.5 22 8062.5 23 10112.0 24 12629.0 25 15872.5 26 20374.0 27 25528.0 28 30649.5 29 36384.0 30 42113.0 31 48677.0 32 56442.0 33 64186.5 34 71862.0 35 78852.5 36 85257.5 37 89202.5 38 90709.5 39 91592.0 40 92006.0 41 92218.0 42 92130.5 43 93055.0 44 95008.0 45 98559.0 46 110054.5 47 147338.0 48 177178.5 49 148906.5 50 104981.0 51 82079.5 52 67958.0 53 58524.5 54 50994.5 55 43965.5 56 37265.0 57 31203.0 58 25980.5 59 21330.0 60 16894.0 61 12818.0 62 9233.0 63 6340.0 64 4422.5 65 3104.0 66 2192.0 67 1636.0 68 1174.0 69 876.0 70 658.5 71 475.5 72 392.0 73 326.5 74 272.5 75 226.5 76 163.0 77 123.5 78 96.5 79 75.5 80 63.0 81 47.0 82 32.5 83 22.5 84 13.5 85 9.0 86 9.5 87 7.5 88 7.0 89 7.5 90 7.0 91 6.0 92 6.0 93 4.5 94 3.5 95 3.0 96 2.5 97 2.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 54.153425261666854 2 0.08913771870976422 3 0.12060688397333716 4 0.1953554950507076 5 0.12243822494473273 6 0.14919822771043137 7 0.1576448207621742 8 0.15547711104093045 9 0.14385370201839937 10-11 0.12933378431662015 12-13 0.14209710965808117 14-15 0.14972146798797295 16-17 0.14325571312978042 18-19 0.1521507978479875 20-21 0.17971434818276782 22-23 0.1985136238687265 24-25 0.2258903026758134 26-27 0.21789220129053477 28-29 0.21682703358268224 30-31 0.23160857142323224 32-33 0.22387209017672444 34-35 0.20802538462832187 36-37 0.19412214296793098 38-39 0.19073976831667996 40-41 0.1905342096362172 42-43 0.18442351068064217 44-45 0.200886892270433 46-47 0.2276095207305929 48-49 0.2298332919101447 50-51 0.23966273426681892 52-53 0.26627323981036277 54-55 0.2533791043995164 56-57 0.23074896239584247 58-59 0.1872452707488129 60-61 0.19688784157779365 62-63 0.2009616408815104 64-65 0.18958116484498072 66-67 0.19952273011827099 68-69 0.1872452707488129 70-71 0.20064395928443154 72-73 0.22088214573362958 74-75 0.2526876797470507 76-77 0.2519028193307383 78-79 0.2465022321803983 80-81 0.25134220474765806 82-83 0.24311985752914728 84-85 0.2120244353209612 86-87 0.22321803982979743 88-89 0.22473169920411415 90-91 0.21230474261250132 92-93 0.2096511669192547 94-95 0.2501649141231895 96-97 0.27845726341597415 98-99 0.2963782429217737 100-101 0.2925847509095972 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2675635.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.98300858037665 #Duplication Level Percentage of deduplicated Percentage of total 1 84.96356846339857 63.70823983110434 2 10.319130734667201 15.475189368391584 3 2.6225303796839996 5.899356538864313 4 0.9512837829245783 2.8532048022962724 5 0.42856933780774326 1.6067709167062172 6 0.214460848092758 0.9648551767616473 7 0.12756571739001193 0.6695682897132017 8 0.08166598303254989 0.4898848885163665 9 0.05773052966400952 0.3895927921031503 >10 0.21509997720194854 2.681195017165724 >50 0.010149568160147594 0.5146666689160846 >100 0.005780715069052239 0.886508754707698 >500 0.0011199831397559863 0.6099446001614279 >1k 0.0012319814537281831 2.3431597496033856 >5k 5.599915698764468E-5 0.3996594178937517 >10k+ 5.599915698764468E-5 0.5082031870947759 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 12103 0.4523412199347071 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9518 0.3557286401172058 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4507 0.16844599506285424 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4305 0.16089638534403983 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4274 0.15973778187234058 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3935 0.14706789229472628 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3916 0.14635778048949127 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3678 0.1374626957712842 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3208 0.11989677216810216 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3189 0.11918666036286714 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3015 0.1126835311991359 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.606145830802781E-5 0.0 12-13 0.0 0.0 0.0 2.0555868046276865E-4 0.0 14-15 0.0 3.737430553868521E-5 0.0 2.6162013877079645E-4 0.0 16-17 0.0 3.737430553868521E-5 0.0 4.858659720029077E-4 0.0 18-19 0.0 3.737430553868521E-5 0.0 6.16676041388306E-4 0.0 20-21 0.0 3.737430553868521E-5 0.0 8.782961801591025E-4 0.0 22-23 0.0 3.737430553868521E-5 0.0 0.0021490225684743995 0.0 24-25 0.0 3.737430553868521E-5 0.0 0.00351318472063641 0.0 26-27 0.0 3.737430553868521E-5 0.0 0.004185922220332743 0.0 28-29 0.0 3.737430553868521E-5 0.0 0.006727374996963338 0.0 30-31 0.0 3.737430553868521E-5 0.0 0.013959303118698927 3.737430553868521E-5 32-33 0.0 3.737430553868521E-5 0.0 0.029955505889256197 3.737430553868521E-5 34-35 0.0 3.737430553868521E-5 0.0 0.05695844164095626 3.737430553868521E-5 36-37 0.0 3.737430553868521E-5 0.0 0.09711713294227352 3.737430553868521E-5 38-39 0.0 3.737430553868521E-5 0.0 0.14682495930872486 3.737430553868521E-5 40-41 0.0 3.737430553868521E-5 0.0 0.20490463011584165 3.737430553868521E-5 42-43 0.0 3.737430553868521E-5 3.737430553868521E-5 0.26569393807451314 3.737430553868521E-5 44-45 0.0 3.737430553868521E-5 3.737430553868521E-5 0.3364621856120136 3.737430553868521E-5 46-47 0.0 3.737430553868521E-5 3.737430553868521E-5 0.42246046265652826 3.737430553868521E-5 48-49 0.0 3.737430553868521E-5 3.737430553868521E-5 0.520026087265266 3.737430553868521E-5 50-51 0.0 3.737430553868521E-5 3.737430553868521E-5 0.6233473549269613 3.737430553868521E-5 52-53 0.0 3.737430553868521E-5 3.737430553868521E-5 0.7173437333567545 3.737430553868521E-5 54-55 0.0 3.737430553868521E-5 3.737430553868521E-5 0.8203660065741403 3.737430553868521E-5 56-57 0.0 3.737430553868521E-5 3.737430553868521E-5 0.9388986165900804 3.737430553868521E-5 58-59 0.0 3.737430553868521E-5 3.737430553868521E-5 1.0917969005488417 3.737430553868521E-5 60-61 0.0 3.737430553868521E-5 3.737430553868521E-5 1.28485387580892 3.737430553868521E-5 62-63 0.0 7.474861107737042E-5 3.737430553868521E-5 1.4599524972576603 3.737430553868521E-5 64-65 0.0 7.474861107737042E-5 3.737430553868521E-5 1.6203069551713893 3.737430553868521E-5 66-67 0.0 7.474861107737042E-5 3.737430553868521E-5 1.8044314714077219 3.737430553868521E-5 68-69 0.0 7.474861107737042E-5 3.737430553868521E-5 2.00800931367694 3.737430553868521E-5 70-71 0.0 7.474861107737042E-5 3.737430553868521E-5 2.2452427180837446 3.737430553868521E-5 72-73 0.0 7.474861107737042E-5 3.737430553868521E-5 2.5034057335922126 3.737430553868521E-5 74-75 0.0 7.474861107737042E-5 3.737430553868521E-5 2.7659041685431682 3.737430553868521E-5 76-77 0.0 7.474861107737042E-5 3.737430553868521E-5 3.0223479659968566 3.737430553868521E-5 78-79 0.0 7.474861107737042E-5 3.737430553868521E-5 3.32584601412375 3.737430553868521E-5 80-81 0.0 7.474861107737042E-5 3.737430553868521E-5 3.6609253504308326 3.737430553868521E-5 82-83 0.0 7.474861107737042E-5 3.737430553868521E-5 3.998377955139621 3.737430553868521E-5 84-85 0.0 7.474861107737042E-5 3.737430553868521E-5 4.395666823015845 3.737430553868521E-5 86-87 0.0 7.474861107737042E-5 3.737430553868521E-5 4.828648152681513 3.737430553868521E-5 88-89 0.0 7.474861107737042E-5 3.737430553868521E-5 5.234420987915018 3.737430553868521E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11890 0.0 29.024826 1 TACATGG 13580 0.0 23.517046 2 ACATGGG 13525 0.0 22.914509 3 GAGTACT 8465 0.0 22.1365 12-13 CATGGGG 3165 0.0 20.745705 4 GTATAGC 1260 0.0 19.622185 1 GTACTTT 9575 0.0 19.621233 14-15 AGTACTT 9070 0.0 19.593098 12-13 CATGGGA 10625 0.0 19.384052 4 GCTTATA 2875 0.0 18.990862 1 TAGCCCT 2050 0.0 18.43415 4 GTACCGT 180 1.2956049E-4 18.355356 6 CTTTGCG 465 0.0 18.287546 2 ATGGGAT 3950 0.0 17.56538 5 GTAGCCC 1300 0.0 17.443832 3 GTATTAG 890 3.274181E-11 17.362326 1 ACTTTTT 12195 0.0 16.39466 16-17 ATGGGAG 3455 0.0 15.983619 5 TACTTTT 11680 0.0 15.296956 14-15 GTGTAGC 1550 0.0 15.286315 1 >>END_MODULE