FastQCFastQC Report
Fri 27 May 2016
SRR939610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2675635
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA284591.0636353613254423No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA250700.9369738398548383No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT153960.5754148080735975No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG117830.4403814421623279No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA117540.4392975873017059No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113780.4252448484191603No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT102180.38189065399428546No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT95910.35845696442152986No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC91210.3408910408183478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85280.3187280776339075No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG75450.2819891352893799No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67160.25100583599780985No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG62120.2321691860063125No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG53550.2001394061596593No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT50240.18776851102635447No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49910.18653515894357786No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG48560.1814896276958554No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA46700.17453800686565993No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT41230.15409426173599913No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39940.1492729763215087No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT38460.1437415791017833No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35760.1336505166063383No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA33580.12550291799890492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG127750.023.0164761
TACATGG124850.022.7517052
ACATGGG123850.021.9034673
AGTACTT95950.021.02922212-13
GAGTACT92350.020.64933412-13
CATGGGA95100.019.8436034
ATCGACG2051.8189894E-1218.98052864-65
GTACTTT101650.018.92244314-15
ATCGGCG901.02950224E-418.91462564-65
GCGTTTA14700.018.63458662-63
GCGTACG1051.5966585E-518.52914868-69
CGTTGAG12800.018.37047662-63
TACTTTT115450.017.49771514-15
TACGCGG1405.5763667E-717.37057364-65
ATGGGAG31700.017.2639355
GTATAGC13050.016.9980471
TAGCCCT21700.016.9580524
CTTTGCG6400.016.9571912
GCGTCGA2800.016.95238186-87
GCTTATA33450.016.7904571
Produced by FastQC (version 0.11.5)