##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939610_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2675635 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.462310442194095 31.0 31.0 34.0 30.0 34.0 2 31.654599375475353 31.0 31.0 34.0 30.0 34.0 3 31.716168311447564 31.0 31.0 34.0 30.0 34.0 4 35.47415062218875 37.0 35.0 37.0 33.0 37.0 5 35.25207399365011 37.0 35.0 37.0 33.0 37.0 6 35.27434272611922 37.0 35.0 37.0 32.0 37.0 7 35.20046418887479 37.0 35.0 37.0 32.0 37.0 8 35.16990770415247 37.0 35.0 37.0 32.0 37.0 9 36.757914663248165 39.0 35.0 39.0 32.0 39.0 10-11 36.63693123314653 39.0 35.0 39.0 32.0 39.0 12-13 36.58100936786968 39.0 35.0 39.0 32.0 39.0 14-15 37.57102781208947 39.0 36.0 41.0 32.0 41.0 16-17 37.54068996705455 39.0 36.0 41.0 32.0 41.0 18-19 37.57857704806523 39.0 36.0 41.0 32.0 41.0 20-21 37.530922379173546 39.0 36.0 41.0 32.0 41.0 22-23 37.403564013776176 39.0 36.0 41.0 32.0 41.0 24-25 37.26341877722484 39.0 36.0 40.5 31.0 41.0 26-27 36.90973114793311 39.0 36.0 40.0 30.5 41.0 28-29 36.70657713028869 39.0 35.5 40.0 30.0 41.0 30-31 36.51984239255354 38.0 35.0 40.0 30.0 41.0 32-33 36.37513786446956 38.0 35.0 40.0 30.0 41.0 34-35 36.370501955610536 38.0 35.0 40.0 30.0 41.0 36-37 36.26506885281438 38.0 35.0 40.0 30.0 41.0 38-39 36.26133441220495 38.0 35.0 40.0 30.0 41.0 40-41 36.14740968031887 38.0 35.0 40.0 30.0 41.0 42-43 36.0264043862485 38.0 35.0 40.0 29.5 41.0 44-45 35.84662313805882 38.0 35.0 40.0 29.0 41.0 46-47 35.55451883384692 38.0 35.0 40.0 27.5 41.0 48-49 35.27463237698714 38.0 34.0 40.0 27.0 41.0 50-51 35.059176606674676 38.0 34.0 40.0 26.0 41.0 52-53 34.77391591154996 38.0 34.0 40.0 26.0 41.0 54-55 34.54761318341254 38.0 33.0 40.0 24.5 41.0 56-57 33.902173502738606 37.5 32.5 40.0 22.0 41.0 58-59 30.915624328430447 35.5 28.0 39.5 8.5 41.0 60-61 30.68367004468098 35.0 26.5 39.0 10.5 40.0 62-63 32.394667060342684 36.0 31.0 39.0 19.0 40.0 64-65 32.39588508896019 36.0 31.0 39.0 16.5 40.0 66-67 27.56084107137184 32.0 19.0 37.5 4.5 39.5 68-69 20.025541600405138 20.5 8.0 32.5 2.0 37.5 70-71 18.05385375807986 16.0 7.5 29.0 2.0 35.5 72-73 19.779418156811374 20.0 7.5 31.0 2.0 36.0 74-75 21.26728608349046 24.0 9.5 32.5 2.0 36.0 76-77 23.111691990873194 27.5 12.5 33.5 2.0 36.0 78-79 25.317401289787284 31.0 14.0 35.0 2.0 37.0 80-81 26.921769038004065 32.5 23.5 35.0 2.0 37.0 82-83 27.95246754508743 33.5 26.5 35.0 2.0 37.0 84-85 27.955875147394917 34.0 27.5 35.0 2.0 36.5 86-87 27.617853145141247 34.0 26.5 35.0 2.0 36.0 88-89 27.087419435012624 33.0 26.0 35.0 2.0 36.0 90-91 25.274175102358882 32.0 16.0 34.5 2.0 35.0 92-93 23.764455166717433 31.0 2.0 34.0 2.0 35.0 94-95 20.008578524350295 28.0 2.0 34.0 2.0 35.0 96-97 17.285882229825816 18.5 2.0 33.0 2.0 35.0 98-99 17.530000915670485 21.5 2.0 33.0 2.0 35.0 100-101 16.710752961446534 17.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3306590705382497 1101 2 0.2784784962545075 1101 3 0.22906367425987284 1101 4 0.32159333503388865 1101 5 0.37270238254202326 1101 6 0.3146612550805159 1101 7 0.27718261443810377 1101 8 0.41956815428640937 1101 9 0.3995823595471961 1101 10-11 0.3731630726721775 1101 12-13 0.36744095601969207 1101 14-15 0.5031795893627518 1101 16-17 0.43190035151103245 1101 18-19 0.428165226335409 1101 20-21 0.4705510104398911 1101 22-23 0.48064428704910256 1101 24-25 0.451041301772662 1101 26-27 0.47933178454523784 1101 28-29 0.3625523640921031 1101 30-31 0.23600154025654518 1101 32-33 0.3117778042322428 1101 34-35 0.28291329750167193 1101 36-37 0.3870488273418644 1101 38-39 0.14190534688719936 1101 40-41 0.2406863204395151 1101 42-43 0.39289244466665707 1101 44-45 0.32515392745834504 1101 46-47 0.2801656705698079 1101 48-49 0.15538201921468442 1101 50-51 0.2192258582626181 1101 52-53 0.09557405955063558 1101 54-55 0.2132651202395337 1101 56-57 -3.3102140267590343 1101 58-59 -9.987339861538459 1101 60-61 -11.66897808279504 1101 62-63 -3.8641565895398386 1101 64-65 -0.9283773931670076 1101 66-67 -1.8934899073831382 1101 68-69 -4.65314051622283 1101 70-71 -4.646638876684154 1101 72-73 -3.2977068437099 1101 74-75 -6.271442824992047 1101 76-77 -4.977789820082954 1101 78-79 1.1579968979561208 1101 80-81 1.7760199507541437 1101 82-83 0.35021181640351884 1101 84-85 0.09019814387179537 1101 86-87 0.18540254294572023 1101 88-89 0.22046596527482976 1101 90-91 1.5686107169506975 1101 92-93 1.102847414703085 1101 94-95 -8.181972129980188 1101 96-97 -9.500804372180063 1101 98-99 -8.458833022928886 1101 100-101 -9.217857146106706 1102 1 0.2860740219840494 1102 2 0.3743256733334732 1102 3 0.36806624283027745 1102 4 0.24166888829914512 1102 5 0.31161988350949343 1102 6 0.334223294778063 1102 7 0.3870579254837523 1102 8 0.3365365093386288 1102 9 0.3234180311952741 1102 10-11 0.3751198280524406 1102 12-13 0.36980249684111044 1102 14-15 0.4528354340133731 1102 16-17 0.40738952782403715 1102 18-19 0.36143377728874526 1102 20-21 0.3394802313870784 1102 22-23 0.349182883779541 1102 24-25 0.29341772566498037 1102 26-27 0.23207292137324487 1102 28-29 0.2982124074830992 1102 30-31 0.4523666271488196 1102 32-33 0.331297861538836 1102 34-35 0.40895358355339084 1102 36-37 0.10941113011235615 1102 38-39 0.31902078317321525 1102 40-41 0.24565214802648683 1102 42-43 0.2682383294399102 1102 44-45 0.10595496651625069 1102 46-47 0.16629902468964985 1102 48-49 0.2552653958922235 1102 50-51 0.44807713482807543 1102 52-53 0.34065673102426075 1102 54-55 0.2058908819379539 1102 56-57 -3.2775150076681996 1102 58-59 -9.841514465174559 1102 60-61 -8.012628891563875 1102 62-63 -2.339136212415152 1102 64-65 -0.7310853451080739 1102 66-67 -1.8023274638525884 1102 68-69 -5.011302193794057 1102 70-71 -4.704851910624974 1102 72-73 -3.326381067374035 1102 74-75 -4.095460537300131 1102 76-77 -3.861325654748029 1102 78-79 1.99448049800505 1102 80-81 2.2778873525679835 1102 82-83 0.9814159365428345 1102 84-85 0.6342955700880566 1102 86-87 0.45595448982583875 1102 88-89 0.37597525804573806 1102 90-91 1.73593072152239 1102 92-93 1.1510750309454352 1102 94-95 -8.282267936653657 1102 96-97 -9.273839044872812 1102 98-99 -8.000289318132015 1102 100-101 -9.009174962079966 1103 1 0.2672020913572517 1103 2 0.19763014337033624 1103 3 0.23108854699497527 1103 4 0.16801805383479973 1103 5 0.15109671130318958 1103 6 0.2774967149788665 1103 7 0.2935463477810032 1103 8 0.2549629994848388 1103 9 0.2967609969902938 1103 10-11 0.30266465089647454 1103 12-13 0.27564323592071105 1103 14-15 0.3637242063517405 1103 16-17 0.34759034857989946 1103 18-19 0.27772072140732007 1103 20-21 0.37426337681922917 1103 22-23 0.38602498007201547 1103 24-25 0.4703574760293634 1103 26-27 0.4882065544913843 1103 28-29 0.42751590940399353 1103 30-31 0.28853868784793946 1103 32-33 0.314422426604942 1103 34-35 0.20223331014341994 1103 36-37 0.18288263424218343 1103 38-39 0.3116037146264148 1103 40-41 0.3187412512189809 1103 42-43 0.28103567325694456 1103 44-45 0.38905182242816494 1103 46-47 0.3511978521882355 1103 48-49 0.3983137826983807 1103 50-51 0.296067709819809 1103 52-53 0.25623913702619205 1103 54-55 0.14007920581656919 1103 56-57 0.5661973274830245 1103 58-59 -8.284997773696269 1103 60-61 -7.234210938174662 1103 62-63 -2.1405548334537556 1103 64-65 -0.2292326302196841 1103 66-67 -1.9625496825036244 1103 68-69 -3.3753012578187587 1103 70-71 -3.887990671154089 1103 72-73 -2.779091548592927 1103 74-75 -3.785642731076237 1103 76-77 -0.4621528874411318 1103 78-79 3.4083287687152044 1103 80-81 2.9010417597600906 1103 82-83 1.4448559233359397 1103 84-85 0.9699850337430469 1103 86-87 0.9137110563840558 1103 88-89 0.6184043038622633 1103 90-91 2.130531843621391 1103 92-93 1.6734091449074882 1103 94-95 -7.232793095737804 1103 96-97 -9.038953682367435 1103 98-99 -8.5364543906701 1103 100-101 -9.693669961527243 1104 1 0.17041092628836196 1104 2 0.2129669134907175 1104 3 0.20796864840081497 1104 4 0.15410880208654731 1104 5 0.18698346327085602 1104 6 0.1727524045496125 1104 7 0.2806420900689801 1104 8 0.275469577867419 1104 9 0.28915412015494013 1104 10-11 0.19730298013644898 1104 12-13 0.2521464945377332 1104 14-15 0.33376562428778556 1104 16-17 0.26847746264276395 1104 18-19 0.32426074587631604 1104 20-21 0.2886647902854378 1104 22-23 0.38150766256919866 1104 24-25 0.31838096377831704 1104 26-27 0.40836588117655026 1104 28-29 0.36767320260086933 1104 30-31 0.40655714463175485 1104 32-33 0.39022274093235865 1104 34-35 0.3667193723556892 1104 36-37 0.2369290601227405 1104 38-39 0.17222691644006005 1104 40-41 0.17963206776524743 1104 42-43 0.185758593691439 1104 44-45 0.21220272692279707 1104 46-47 0.18618974940882538 1104 48-49 0.10419128325351323 1104 50-51 0.17687400231503858 1104 52-53 0.22407525129863615 1104 54-55 0.137699089303446 1104 56-57 0.8131226356356365 1104 58-59 -8.018485632955858 1104 60-61 -7.480633269712865 1104 62-63 -2.096635203241682 1104 64-65 -0.30331383162759096 1104 66-67 -1.6666680238486151 1104 68-69 -3.0740146688179166 1104 70-71 -3.5825869877586243 1104 72-73 -2.584779512692265 1104 74-75 -3.2302313741400503 1104 76-77 0.0317177520094063 1104 78-79 3.7791031057093 1104 80-81 3.117989301996939 1104 82-83 1.5000403113255025 1104 84-85 1.1786603791842687 1104 86-87 1.1967380955435765 1104 88-89 0.9430972303444705 1104 90-91 2.558503074558036 1104 92-93 2.1125731941773758 1104 94-95 -6.349583786006363 1104 96-97 -7.841795122556743 1104 98-99 -5.934758873888306 1104 100-101 -5.268446577562928 1105 1 0.20743061466106028 1105 2 0.17618761733915989 1105 3 0.20821808060652103 1105 4 0.24116777870796113 1105 5 0.2999612651129624 1105 6 0.27262389403218634 1105 7 0.25976376992648653 1105 8 0.23105565790886828 1105 9 0.2992792597096994 1105 10-11 0.21864953579527935 1105 12-13 0.16370707658903427 1105 14-15 0.30416609798297856 1105 16-17 0.27219253945765587 1105 18-19 0.36678281117022493 1105 20-21 0.31171883250263477 1105 22-23 0.5574151759144712 1105 24-25 0.5346687797584835 1105 26-27 0.44159989759174323 1105 28-29 0.3718578233509149 1105 30-31 0.4199771206662817 1105 32-33 0.2630375922947863 1105 34-35 0.3076537784655713 1105 36-37 0.28978364935416323 1105 38-39 0.4124180573979217 1105 40-41 0.4764353983810352 1105 42-43 0.34617246559533044 1105 44-45 0.38600100965878426 1105 46-47 0.3961935271708015 1105 48-49 0.38241679137796325 1105 50-51 0.3856282995787552 1105 52-53 0.32765771391889587 1105 54-55 0.31493542090625937 1105 56-57 0.8302978331533524 1105 58-59 -8.323441245442172 1105 60-61 -7.406996885963618 1105 62-63 -1.9041777976268506 1105 64-65 -0.08324569631233913 1105 66-67 -1.7221295781026775 1105 68-69 -3.3868997751340864 1105 70-71 -3.6832841489893653 1105 72-73 -2.7544533619438596 1105 74-75 -3.5164296728625324 1105 76-77 -0.004088021067474301 1105 78-79 3.7711696866229865 1105 80-81 3.1915563395487965 1105 82-83 1.7883743064015682 1105 84-85 1.3986765940618433 1105 86-87 1.1851485379627995 1105 88-89 1.0818758023456851 1105 90-91 2.331822190533188 1105 92-93 2.1768849527945626 1105 94-95 -6.15355856016733 1105 96-97 -7.281457686101291 1105 98-99 -5.285705229430423 1105 100-101 -4.847419406937741 1106 1 0.05294951489173272 1106 2 0.19659710784515028 1106 3 0.21651871192046457 1106 4 0.18432546330731725 1106 5 0.23765197726301324 1106 6 0.1806413500378099 1106 7 0.2709845349405313 1106 8 0.15154490537054244 1106 9 0.14604370726056715 1106 10-11 0.06582360725684566 1106 12-13 0.19622268672617338 1106 14-15 0.19756829434850687 1106 16-17 0.3690330396014261 1106 18-19 0.26413277435290894 1106 20-21 0.23808854744298458 1106 22-23 0.26273031713407136 1106 24-25 0.21812859742760082 1106 26-27 0.2620315666299149 1106 28-29 0.2443116694919425 1106 30-31 0.31145781283659346 1106 32-33 0.3766739830019148 1106 34-35 0.4369780264620644 1106 36-37 0.37429820988214146 1106 38-39 0.42683940377061447 1106 40-41 0.3386974228498403 1106 42-43 0.3902013782064415 1106 44-45 0.420240994717453 1106 46-47 0.48660546898824464 1106 48-49 0.4387721796059054 1106 50-51 0.34371629332207476 1106 52-53 0.40439364859633287 1106 54-55 0.39503619148518254 1106 56-57 1.0670581904206813 1106 58-59 -8.062158899863476 1106 60-61 -7.345036415683449 1106 62-63 -1.8890647121261068 1106 64-65 -0.0863952980159226 1106 66-67 0.7422906724456517 1106 68-69 -2.3023937310671734 1106 70-71 -2.3809568086241732 1106 72-73 -0.9493882473456488 1106 74-75 -0.8916851964724266 1106 76-77 2.3881761855211465 1106 78-79 4.47144731134269 1106 80-81 3.556222209598797 1106 82-83 1.7748308106313324 1106 84-85 1.3453648579973354 1106 86-87 1.1472004976660024 1106 88-89 1.107290887511489 1106 90-91 2.5523417160984145 1106 92-93 2.121216717907945 1106 94-95 -5.832224205811558 1106 96-97 -7.489884134727497 1106 98-99 -5.086330051805632 1106 100-101 -4.490747277824855 1107 1 0.03517381774383921 1107 2 0.011059437612608747 1107 3 0.013361391781277376 1107 4 0.018519258507154746 1107 5 -0.08677368913964756 1107 6 0.03215103270366626 1107 7 -0.06757822592677343 1107 8 0.030005529688061472 1107 9 -0.015364845842270825 1107 10-11 0.036766444450414326 1107 12-13 0.04054753066107253 1107 14-15 0.08586983380036628 1107 16-17 0.0798115594070481 1107 18-19 0.06255390003080663 1107 20-21 0.08223931121745665 1107 22-23 0.025682357186909144 1107 24-25 0.011327318292536859 1107 26-27 0.061545380084069734 1107 28-29 0.0016413667222039408 1107 30-31 0.06003303367736379 1107 32-33 0.08629958627150103 1107 34-35 -0.016401640165803144 1107 36-37 -0.06642663252919334 1107 38-39 -0.029946163514075863 1107 40-41 -0.20063576622013812 1107 42-43 -0.1873937374077883 1107 44-45 -0.2285367780610983 1107 46-47 -0.15778107966814048 1107 48-49 -0.28224832412077916 1107 50-51 -0.26629373845633353 1107 52-53 -0.11119655396989003 1107 54-55 -0.07062371456270711 1107 56-57 0.40920884754501685 1107 58-59 -8.921098204747409 1107 60-61 -8.060973124065075 1107 62-63 -2.7378806456564106 1107 64-65 -0.8332474525986271 1107 66-67 0.10928807356276238 1107 68-69 -3.003561716696069 1107 70-71 -2.8161612566966276 1107 72-73 -1.3878933742890673 1107 74-75 -1.361542130552408 1107 76-77 1.8362235834125684 1107 78-79 3.8935598600881107 1107 80-81 2.80456199074224 1107 82-83 1.2261121120296146 1107 84-85 0.6991268106089663 1107 86-87 0.6392889858550923 1107 88-89 0.5600771937292635 1107 90-91 2.082298620397296 1107 92-93 1.372589302776337 1107 94-95 -7.330876974156274 1107 96-97 -8.417897733095941 1107 98-99 -6.426071379995001 1107 100-101 -5.576542670015257 1108 1 -0.08885086468038494 1108 2 -0.1988480267304169 1108 3 -0.1805061759332247 1108 4 -0.06360953496735533 1108 5 -0.0440940544830184 1108 6 -0.09985381904431279 1108 7 -0.15622280632931052 1108 8 -0.15883617640999148 1108 9 -0.11634630309139737 1108 10-11 -0.196706585979868 1108 12-13 -0.15106153946430823 1108 14-15 -0.26776339992340326 1108 16-17 -0.18050543520878648 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-0.4375052777679045 2312 34-35 -0.44533870626130323 2312 36-37 -0.4822455782381283 2312 38-39 -0.4717601807063545 2312 40-41 -0.3970142901061635 2312 42-43 -0.4556843073727137 2312 44-45 -0.6262214400777992 2312 46-47 -0.6030795306123053 2312 48-49 -0.5735399356933897 2312 50-51 -0.5961310382987932 2312 52-53 -0.5023622566738126 2312 54-55 -0.6138555093833631 2312 56-57 0.26299043389749954 2312 58-59 3.2452619054459753 2312 60-61 3.332340034370514 2312 62-63 0.675754578686302 2312 64-65 0.006834465998956318 2312 66-67 -2.165304313113051 2312 68-69 -5.0529687651110855 2312 70-71 -5.019848631360226 2312 72-73 -7.497203185682949 2312 74-75 -7.8604361132996665 2312 76-77 -5.365975208214234 2312 78-79 -0.6874197226416392 2312 80-81 1.0017500335282499 2312 82-83 -0.037360543005124924 2312 84-85 -0.22005564580289771 2312 86-87 -0.1968726345679066 2312 88-89 -0.0439843655779697 2312 90-91 1.6660469123028285 2312 92-93 3.1788229197900293 2312 94-95 5.0521446401619166 2312 96-97 -0.05800953277156751 2312 98-99 -1.253798222949122 2312 100-101 -0.6504381586605597 2313 1 -0.274333110224827 2313 2 -0.3791108654456181 2313 3 -0.3144978917691894 2313 4 -0.255502554294992 2313 5 -0.3205990986462055 2313 6 -0.2957423266283925 2313 7 -0.3068044770032401 2313 8 -0.3391196385823392 2313 9 -0.3503743830988171 2313 10-11 -0.3796212668491492 2313 12-13 -0.347932002303736 2313 14-15 -0.439537623678774 2313 16-17 -0.42716242854507414 2313 18-19 -0.4403950701843513 2313 20-21 -0.3761987636344628 2313 22-23 -0.29342437506676333 2313 24-25 -0.39805759347212444 2313 26-27 -0.5056772501292883 2313 28-29 -0.4539751541676722 2313 30-31 -0.4930005995719071 2313 32-33 -0.5875567495540395 2313 34-35 -0.5112662727083048 2313 36-37 -0.5049392054171165 2313 38-39 -0.5749599313751332 2313 40-41 -0.5098455216748974 2313 42-43 -0.5442547375399016 2313 44-45 -0.5919702719279627 2313 46-47 -0.641361081282497 2313 48-49 -0.6560842710816601 2313 50-51 -0.6915456813136274 2313 52-53 -0.307356790747896 2313 54-55 -0.5308785040496602 2313 56-57 0.3031325243603433 2313 58-59 3.2802461923846558 2313 60-61 3.2253353853821203 2313 62-63 0.6628252437820592 2313 64-65 -0.09132966761046646 2313 66-67 -2.069361966235711 2313 68-69 -4.923238023940977 2313 70-71 -3.652206086387343 2313 72-73 -2.6947863728253942 2313 74-75 -3.4623030280383063 2313 76-77 0.5084655083296958 2313 78-79 3.0600385627812194 2313 80-81 2.4294144658156966 2313 82-83 0.909687857001483 2313 84-85 0.568643227720532 2313 86-87 0.43424170599115186 2313 88-89 0.4582911359768147 2313 90-91 2.1722677970981117 2313 92-93 3.3997330968010324 2313 94-95 5.434739384845329 2313 96-97 1.178510407823378 2313 98-99 2.965981581694198 2313 100-101 4.112664126167305 2314 1 -0.27812985018763214 2314 2 -0.280775562962301 2314 3 -0.4168857049187338 2314 4 -0.28152891902532673 2314 5 -0.2420215907149057 2314 6 -0.31278860165301126 2314 7 -0.3311258860394517 2314 8 -0.5124385314824593 2314 9 -0.5070184045406734 2314 10-11 -0.4792247019219005 2314 12-13 -0.6080547459860384 2314 14-15 -0.7374355013726799 2314 16-17 -0.5902047105711077 2314 18-19 -0.46419055207911697 2314 20-21 -0.5706806036957346 2314 22-23 -0.5336389030177529 2314 24-25 -0.5894462817994466 2314 26-27 -0.71175656311474 2314 28-29 -0.6937293933099937 2314 30-31 -0.5296436364387702 2314 32-33 -0.638868805127828 2314 34-35 -0.7215088610465656 2314 36-37 -0.5703532738997765 2314 38-39 -0.5561868212121368 2314 40-41 -0.6392176910217913 2314 42-43 -0.8043721207583658 2314 44-45 -0.5691889569733632 2314 46-47 -0.6259990858771829 2314 48-49 -0.6258347907184572 2314 50-51 -0.7055324989484575 2314 52-53 -0.5864369242124994 2314 54-55 -0.6426981080317233 2314 56-57 0.11997986053800247 2314 58-59 3.2457962416134087 2314 60-61 3.2464459859728656 2314 62-63 0.7273773168593962 2314 64-65 -0.2594580999482865 2314 66-67 -2.1409101557116976 2314 68-69 -5.06321740256476 2314 70-71 -3.7443184373336287 2314 72-73 -2.9652512563173516 2314 74-75 -4.1489370943330215 2314 76-77 0.6160826727537199 2314 78-79 3.027726124317155 2314 80-81 2.2954144111170827 2314 82-83 0.8614902309211878 2314 84-85 0.46959881244868384 2314 86-87 0.3588944245121013 2314 88-89 0.49245927950596524 2314 90-91 2.165907278555281 2314 92-93 3.7115069180459734 2314 94-95 6.2109003628564885 2314 96-97 3.690527528488513 2314 98-99 5.818726193881048 2314 100-101 6.221577428869415 2315 1 -0.4565067498836797 2315 2 -0.4055790077444712 2315 3 -0.45961890316257126 2315 4 -0.3314961598223576 2315 5 -0.49119990885066755 2315 6 -0.36202196875871806 2315 7 -0.4595412862420858 2315 8 -0.5727330265854604 2315 9 -0.6648897317949789 2315 10-11 -0.6482638778759693 2315 12-13 -0.6850821016091402 2315 14-15 -0.8187342180224633 2315 16-17 -0.8072734373525989 2315 18-19 -0.8041755233725922 2315 20-21 -0.7507115054181241 2315 22-23 -0.6983396797823644 2315 24-25 -0.6343081527889751 2315 26-27 -0.6314826691599933 2315 28-29 -0.8004624226919574 2315 30-31 -0.6027493439700109 2315 32-33 -0.6863482039795699 2315 34-35 -0.571094305153288 2315 36-37 -0.5232576980807977 2315 38-39 -0.6986066118234149 2315 40-41 -0.8428951648482084 2315 42-43 -0.6471978553075104 2315 44-45 -0.6131349211746482 2315 46-47 -0.38685825507677407 2315 48-49 -0.45417673600721287 2315 50-51 -0.5334880882763855 2315 52-53 -0.5188916692310741 2315 54-55 -0.4854866929692463 2315 56-57 0.07376861433739634 2315 58-59 3.2797533506981758 2315 60-61 3.5777826965436184 2315 62-63 0.8241417545493661 2315 64-65 0.04756722933236546 2315 66-67 -1.8617943164681954 2315 68-69 -4.609289168859931 2315 70-71 -3.289242787215427 2315 72-73 -2.9957445018425126 2315 74-75 -0.5030532713002636 2315 76-77 2.009936607235314 2315 78-79 3.878191507633602 2315 80-81 2.579367298655079 2315 82-83 0.9581010043085563 2315 84-85 0.5966386638502357 2315 86-87 0.5980837186694821 2315 88-89 0.9105778205394017 2315 90-91 2.6809263599467066 2315 92-93 4.062937346955124 2315 94-95 6.452495263227984 2315 96-97 4.020544921261223 2315 98-99 6.1898141709158345 2315 100-101 6.361527952341218 2316 1 -0.4271944932455298 2316 2 -0.35501783390103014 2316 3 -0.5277834002531918 2316 4 -0.25211183077350086 2316 5 -0.5097916862742053 2316 6 -0.6287076573022503 2316 7 -0.5526447168432753 2316 8 -0.44439356239870165 2316 9 -0.5480094107239211 2316 10-11 -0.4902842859578769 2316 12-13 -0.5266281018039223 2316 14-15 -0.5515133540989297 2316 16-17 -0.6381695784744252 2316 18-19 -0.6303931940127825 2316 20-21 -0.6373127003792547 2316 22-23 -0.784330864794029 2316 24-25 -0.6836025493654958 2316 26-27 -0.6754164844188466 2316 28-29 -0.6235065152886321 2316 30-31 -0.7183867505478219 2316 32-33 -0.5041996089502092 2316 34-35 -0.3673365732444296 2316 36-37 -0.25379902994249903 2316 38-39 -0.567370070228435 2316 40-41 -0.4920962744956583 2316 42-43 -0.467819563141731 2316 44-45 -0.4111428636625192 2316 46-47 -0.5534054939716171 2316 48-49 -0.4765652572726964 2316 50-51 -0.5645732521806082 2316 52-53 -0.5071179247746116 2316 54-55 -0.36854577526050747 2316 56-57 0.38221791562513374 2316 58-59 3.446826595744941 2316 60-61 3.4377512136288537 2316 62-63 0.9414360577250562 2316 64-65 0.3765610119123082 2316 66-67 -2.0007089190618927 2316 68-69 -2.84824916198016 2316 70-71 -3.024823777412326 2316 72-73 -0.690759688020556 2316 74-75 5.612455823036289 2316 76-77 5.804395488942593 2316 78-79 4.762404033254761 2316 80-81 3.066434928261547 2316 82-83 1.3750739858396805 2316 84-85 0.8803548551693119 2316 86-87 0.8914916701557196 2316 88-89 0.7612681609000091 2316 90-91 2.207019420207896 2316 92-93 3.939041769085879 2316 94-95 6.45771739489696 2316 96-97 3.7479353418668175 2316 98-99 5.992092410856877 2316 100-101 6.009490020212867 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 47.0 5 127.0 6 256.0 7 578.0 8 1271.0 9 2248.0 10 3785.0 11 5970.0 12 8619.0 13 12205.0 14 15192.0 15 20292.0 16 26540.0 17 35204.0 18 38034.0 19 36848.0 20 38796.0 21 40248.0 22 40366.0 23 41083.0 24 43866.0 25 46730.0 26 57456.0 27 80331.0 28 113342.0 29 150091.0 30 183688.0 31 208035.0 32 220753.0 33 221473.0 34 225389.0 35 240571.0 36 242340.0 37 189277.0 38 79420.0 39 5152.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6870349663787 20.377757864565986 14.628621715394843 22.306585453660475 2 14.13814138888972 22.94134313763602 40.561875955037365 22.358639518436892 3 19.803687209133706 27.407061071269993 31.036078259010598 21.7531734605857 4 11.658839864181774 17.695014454512666 40.5030581525507 30.143087528754858 5 11.792399204797542 40.064456873430274 35.52816790798628 12.614976013785903 6 27.984123395007167 39.043853141403815 18.941298047005663 14.030725416583353 7 25.414527766305945 34.84757823843685 22.463153606527047 17.27474038873015 8 23.26389810269338 35.49751741175459 21.631836928430072 19.606747557121952 9 25.27538322678542 18.122875504319534 21.997955625487034 34.60378564340802 10-11 22.768595118541953 27.09291439228445 29.52439327486746 20.61409721430614 12-13 26.26512958606088 24.695801183644257 28.74687690959342 20.292192320701442 14-15 22.61436000685449 26.20986512090569 28.765068712150104 22.410706160089713 16-17 19.91355695256583 29.581768361859588 29.74944821499349 20.75522647058109 18-19 19.54668858306592 29.972245705858303 31.013461542773978 19.4676041683018 20-21 21.14871796788426 28.452591627781814 29.597609539417746 20.80108086491618 22-23 20.154355881874768 28.414806204882204 29.126375608033232 22.30446230520979 24-25 20.803305386670253 28.194735263531147 30.229543855518276 20.772415494280324 26-27 21.670147152121974 27.309374512381652 30.691060898684704 20.32941743681167 28-29 20.260733163926417 28.105620066310138 31.24675541157172 20.386891358191725 30-31 20.337962808094108 29.14539573235149 30.519399820930765 19.997241638623635 32-33 20.118348392565775 28.85568085552911 29.977250900348928 21.048719851556193 34-35 20.99217761170015 27.790212213046882 29.940355200227835 21.27725497502513 36-37 21.478050932616313 27.671716214879254 30.654315443934642 20.195917408569787 38-39 21.912941061183698 27.099265906329094 31.123634210627692 19.86415882185951 40-41 20.853958590642534 27.813126752850753 31.410524473144214 19.922390183362495 42-43 20.0019525686658 28.993250733195147 30.418868995173376 20.585927702965677 44-45 20.542951659055326 29.167323622912168 29.28811005518058 21.001614662851924 46-47 20.53814025932055 28.576523650357146 28.52956182428085 22.355774266041458 48-49 21.092918411113796 28.09725543642907 29.290573215913994 21.51925293654314 50-51 21.796408036627227 28.743242909683314 29.233321290809013 20.227027762880454 52-53 20.791296863842692 28.95004761385676 29.87094990064943 20.387705621651115 54-55 19.85688576025654 28.32126256418809 30.472967995791155 21.34888367976422 56-57 20.955137113869363 29.383458820959852 28.634740500165307 21.02666356500548 58-59 20.25257718179932 28.258359345792456 30.253277456902023 21.2357860155062 60-61 20.264161387539207 27.791587098477542 30.50959393596642 21.43465757801683 62-63 22.342273619012342 27.644906668380948 29.188018838623737 20.824800873982973 64-65 21.618641727111303 28.218459561614285 29.758475610416994 20.404423100857418 66-67 20.92917123037605 27.729192697762628 30.368982045252345 20.97265402660898 68-69 20.927147981721088 28.150779384705455 30.541611058324 20.380461575249456 70-71 21.82537707869848 27.88479941449642 29.068572360372553 21.221251146432547 72-73 20.86490301542411 27.462606510203624 29.43836769436982 22.234122780002444 74-75 20.93808417982809 28.136940365900802 29.60845349980632 21.316521954464783 76-77 20.117943804420086 27.71039264457219 30.46063867462523 21.71102487638249 78-79 20.7681793057383 27.62609079217387 30.07048390755582 21.535245994532016 80-81 20.257912958068815 29.319806497046553 29.248393535519256 21.17388700936538 82-83 20.42053265576095 28.99537137134593 28.751204622611382 21.832891350281738 84-85 20.72227564743446 28.120618971676997 28.56575293980899 22.59135244107955 86-87 21.048840989370238 28.29700691545563 29.04463333658494 21.609518758589193 88-89 21.820562561889524 27.279136967324945 29.251117818969668 21.64918265181586 90-91 20.780202616425694 27.719580816878324 29.817746468837836 21.682470097858143 92-93 20.047223038171268 28.91629522027539 29.552467476665672 21.484014264887662 94-95 19.568230645306762 29.034200326278064 29.19345240004574 22.204116628369437 96-97 19.338928794325376 28.403628814318218 28.810251233208483 23.447191158147916 98-99 20.219193779045284 28.06044898478938 28.845930665065605 22.87442657109973 100-101 20.216035924416182 28.28983870700971 29.343573465972394 22.15055190260171 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1598.0 1 1138.5 2 710.0 3 750.0 4 775.5 5 839.0 6 1112.5 7 1662.0 8 2242.5 9 2585.0 10 2672.5 11 2673.0 12 2751.0 13 2862.0 14 2973.5 15 3160.5 16 3397.0 17 3693.0 18 4097.5 19 4660.0 20 5462.0 21 6470.5 22 7826.0 23 9562.0 24 11933.0 25 15036.5 26 18969.0 27 23809.0 28 29193.0 29 34952.5 30 40964.0 31 47679.5 32 55409.0 33 63430.5 34 71235.5 35 78507.0 36 85122.5 37 90554.0 38 93406.5 39 94862.5 40 95943.0 41 96454.5 42 97254.5 43 98620.5 44 101870.5 45 107662.5 46 117731.0 47 134594.0 48 158436.5 49 148412.0 50 111680.0 51 88374.0 52 71393.5 53 60000.5 54 50862.0 55 43110.0 56 36451.0 57 30017.0 58 24322.0 59 19523.0 60 15170.0 61 11221.0 62 8027.0 63 5586.5 64 3790.5 65 2529.0 66 1699.0 67 1197.0 68 887.0 69 667.0 70 500.5 71 356.0 72 295.5 73 268.5 74 210.5 75 164.0 76 120.0 77 88.5 78 64.0 79 43.0 80 29.0 81 21.5 82 14.5 83 9.5 84 8.5 85 7.5 86 4.0 87 1.5 88 0.0 89 1.0 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025937768043847533 2 0.012968884021923767 3 2.6162013877079645E-4 4 0.0 5 0.05976151455635765 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.166760413883059E-4 16-17 1.8687152769342606E-5 18-19 4.858659720029077E-4 20-21 0.0 22-23 0.0 24-25 9.343576384671303E-5 26-27 9.343576384671303E-5 28-29 9.717319440058154E-4 30-31 0.0049894697894144755 32-33 0.005400587150340013 34-35 0.001700530902010177 36-37 0.08078456142186807 38-39 0.07467386246629305 40-41 0.07971939371401555 42-43 0.08347551142065342 44-45 0.08676445030805773 46-47 0.08173760621310455 48-49 0.07149704649550481 50-51 0.06641414094224363 52-53 0.05675288296049349 54-55 0.047390619423052845 56-57 0.06723637566409468 58-59 0.7301444330037542 60-61 0.3423112644288178 62-63 0.0025414527766305945 64-65 0.0017192180547795197 66-67 1.1122967071368106 68-69 2.3916378728787744 70-71 2.3614207468507478 72-73 1.8228943783438325 74-75 1.2948701896932877 76-77 0.7461219486215422 78-79 0.5696031035623319 80-81 0.03926170796838881 82-83 1.6818437492408344E-4 84-85 0.0013454749993926675 86-87 0.0012707263883152973 88-89 1.8687152769342606E-5 90-91 0.3371162359589406 92-93 0.05667813434941612 94-95 0.9623136190100667 96-97 1.6703698374404579 98-99 0.6500699833871212 100-101 0.5749289421015945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2675635.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.17481317633893 #Duplication Level Percentage of deduplicated Percentage of total 1 73.38862710452631 39.0242653555119 2 14.769712751740741 15.707534324840095 3 5.435431730141834 8.6708420034911 4 2.5144877755627486 5.348296707989489 5 1.3186050297268552 3.5058288054553186 6 0.7345451386762918 2.3435580311219875 7 0.4926152150852682 1.833630542098681 8 0.2912275770829768 1.2388777602548111 9 0.20910518892065774 1.0007216419553129 >10 0.7770891228815818 6.883632424071946 >50 0.03814231744255414 1.3989761202158768 >100 0.024339477220451402 2.401946254879921 >500 0.002117989880799354 0.7910314414951403 >1k 0.0028945861704042135 3.3881343375250257 >5k 5.647973015422855E-4 2.1808456408849075 >10k+ 4.941976388494998E-4 4.281878608208546 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 28459 1.0636353613254423 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 25070 0.9369738398548383 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 15396 0.5754148080735975 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 11783 0.4403814421623279 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 11754 0.4392975873017059 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11378 0.4252448484191603 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 10218 0.38189065399428546 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9591 0.35845696442152986 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 9121 0.3408910408183478 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8528 0.3187280776339075 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7545 0.2819891352893799 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6716 0.25100583599780985 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6212 0.2321691860063125 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5355 0.2001394061596593 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5024 0.18776851102635447 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4991 0.18653515894357786 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4856 0.1814896276958554 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4670 0.17453800686565993 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4123 0.15409426173599913 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3994 0.1492729763215087 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3846 0.1437415791017833 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3576 0.1336505166063383 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3358 0.12550291799890492 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.737430553868521E-5 0.0 3 0.0 0.0 0.0 3.737430553868521E-5 0.0 4 0.0 0.0 0.0 3.737430553868521E-5 0.0 5 0.0 0.0 0.0 3.737430553868521E-5 0.0 6 0.0 0.0 0.0 3.737430553868521E-5 0.0 7 0.0 0.0 0.0 3.737430553868521E-5 0.0 8 0.0 0.0 0.0 3.737430553868521E-5 0.0 9 0.0 0.0 0.0 3.737430553868521E-5 0.0 10-11 0.0 0.0 0.0 1.1212291661605564E-4 0.0 12-13 0.0 0.0 0.0 2.2424583323211124E-4 0.0 14-15 0.0 0.0 0.0 2.803072915401391E-4 0.0 16-17 0.0 0.0 0.0 5.419274303109355E-4 0.0 18-19 0.0 0.0 0.0 7.10111805235019E-4 0.0 20-21 0.0 0.0 0.0 9.530447912364728E-4 0.0 22-23 0.0 0.0 0.0 0.0023358940961678257 0.0 24-25 0.0 0.0 0.0 0.003812179164945891 0.0 26-27 0.0 0.0 0.0 0.00452229097018091 0.0 28-29 0.0 0.0 0.0 0.0073066767328129585 0.0 30-31 0.0 0.0 0.0 0.014874973604396713 0.0 32-33 0.0 0.0 0.0 0.03176815970788243 0.0 34-35 0.0 0.0 0.0 0.060172631917283184 0.0 36-37 0.0 0.0 0.0 0.10287277599523104 0.0 38-39 0.0 0.0 0.0 0.1552154909021597 0.0 40-41 0.0 0.0 0.0 0.21630379330514066 0.0 42-43 0.0 0.0 0.0 0.2801577943179843 0.0 44-45 0.0 0.0 0.0 0.35376648907642483 0.0 46-47 0.0 0.0 0.0 0.44282945917511174 0.0 48-49 0.0 5.606145830802782E-5 0.0 0.5372930164241385 0.0 50-51 0.0 7.474861107737042E-5 0.0 0.6324106240200924 0.0 52-53 0.0 7.474861107737042E-5 0.0 0.716764431620905 0.0 54-55 0.0 7.474861107737042E-5 0.0 0.812218407966707 0.0 56-57 0.0 7.474861107737042E-5 0.0 0.9010384450793925 0.0 58-59 0.0 7.474861107737042E-5 0.0 0.9780108273363146 0.0 60-61 0.0 7.474861107737042E-5 0.0 1.0519372036918337 0.0 62-63 3.737430553868521E-5 7.474861107737042E-5 0.0 1.1118669026231156 1.8687152769342606E-5 64-65 5.606145830802782E-5 7.474861107737042E-5 0.0 1.1708061824576221 3.737430553868521E-5 66-67 7.474861107737042E-5 7.474861107737042E-5 0.0 1.2213736178514634 3.737430553868521E-5 68-69 7.474861107737042E-5 7.474861107737042E-5 0.0 1.2839382053232224 3.737430553868521E-5 70-71 7.474861107737042E-5 7.474861107737042E-5 0.0 1.3839331597919746 3.737430553868521E-5 72-73 7.474861107737042E-5 7.474861107737042E-5 0.0 1.52391862118712 3.737430553868521E-5 74-75 7.474861107737042E-5 7.474861107737042E-5 0.0 1.707239589854371 3.737430553868521E-5 76-77 7.474861107737042E-5 7.474861107737042E-5 0.0 1.9241226848953614 3.737430553868521E-5 78-79 7.474861107737042E-5 7.474861107737042E-5 0.0 2.206261317406896 3.737430553868521E-5 80-81 7.474861107737042E-5 7.474861107737042E-5 0.0 2.542050765519213 3.737430553868521E-5 82-83 7.474861107737042E-5 7.474861107737042E-5 0.0 2.8680668327331644 3.737430553868521E-5 84-85 7.474861107737042E-5 7.474861107737042E-5 0.0 3.2070144096635005 3.737430553868521E-5 86-87 7.474861107737042E-5 7.474861107737042E-5 0.0 3.53420776750192 3.737430553868521E-5 88-89 7.474861107737042E-5 7.474861107737042E-5 0.0 3.812552908001278 3.737430553868521E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12775 0.0 23.016476 1 TACATGG 12485 0.0 22.751705 2 ACATGGG 12385 0.0 21.903467 3 AGTACTT 9595 0.0 21.029222 12-13 GAGTACT 9235 0.0 20.649334 12-13 CATGGGA 9510 0.0 19.843603 4 ATCGACG 205 1.8189894E-12 18.980528 64-65 GTACTTT 10165 0.0 18.922443 14-15 ATCGGCG 90 1.02950224E-4 18.914625 64-65 GCGTTTA 1470 0.0 18.634586 62-63 GCGTACG 105 1.5966585E-5 18.529148 68-69 CGTTGAG 1280 0.0 18.370476 62-63 TACTTTT 11545 0.0 17.497715 14-15 TACGCGG 140 5.5763667E-7 17.370573 64-65 ATGGGAG 3170 0.0 17.263935 5 GTATAGC 1305 0.0 16.998047 1 TAGCCCT 2170 0.0 16.958052 4 CTTTGCG 640 0.0 16.957191 2 GCGTCGA 280 0.0 16.952381 86-87 GCTTATA 3345 0.0 16.790457 1 >>END_MODULE