FastQCFastQC Report
Fri 27 May 2016
SRR939609_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939609_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337989
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92480.27705304001900544No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62630.1876279400561236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57200.17136066056538832No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA44130.13220534878934592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42730.12801120674753572No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35060.10503329998990409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG131650.027.3285881
TACATGG129450.027.169712
ACATGGG132900.025.1072543
GAGTACT82700.021.94942312-13
CATGGGG59850.021.0950154
GTACTTT91150.020.07096114-15
CATGGGA79400.019.9060964
GTATCAA251450.019.8390081
AGTACTT90550.019.3914612-13
GGACCGT3306.0754246E-1018.693656
TATCAAC278200.017.8802262
TCAACGC276900.017.8267734
ATCAACG277400.017.7949073
CAACGCA277450.017.7398455
AACGCAG282900.017.4112156
GTATTAG10200.017.2175141
AGTACAT98350.016.7947562
ACTTTTT118300.016.4097116-17
ACGCAGA296700.016.313757
GGACCGA5250.016.2696366
Produced by FastQC (version 0.11.5)