##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939609_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3337989 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.055134094210615 31.0 30.0 33.0 26.0 34.0 2 30.194588418356084 31.0 30.0 34.0 26.0 34.0 3 30.233339894169813 31.0 30.0 34.0 26.0 34.0 4 33.80697389955449 35.0 35.0 37.0 28.0 37.0 5 33.79875607738671 35.0 35.0 37.0 28.0 37.0 6 33.89712428650903 35.0 35.0 37.0 28.0 37.0 7 33.70175935271207 35.0 33.0 37.0 28.0 37.0 8 33.70086450254929 35.0 33.0 37.0 28.0 37.0 9 34.95115022847589 37.0 34.0 39.0 28.0 39.0 10-11 34.83469957510346 37.0 34.0 39.0 27.0 39.0 12-13 34.68104912868197 37.0 34.0 39.0 27.0 39.0 14-15 35.341180722884346 38.0 34.0 40.0 27.0 41.0 16-17 35.07339868405798 38.0 33.0 40.0 26.0 41.0 18-19 35.035225550473655 38.0 33.5 40.0 26.0 41.0 20-21 35.15906792982241 38.0 34.0 40.0 26.0 41.0 22-23 35.01521754565398 38.0 34.0 40.0 25.0 41.0 24-25 34.889169047591224 38.0 33.0 40.0 25.0 41.0 26-27 35.006208678338965 38.0 34.0 40.0 25.0 41.0 28-29 34.88448014657927 38.0 34.0 40.0 25.0 41.0 30-31 34.666307168777365 38.0 33.0 40.0 24.0 41.0 32-33 34.44305134019315 38.0 33.0 40.0 24.0 41.0 34-35 34.21427946587002 38.0 33.0 40.0 23.5 41.0 36-37 33.923860294326914 38.0 33.0 40.0 22.5 41.0 38-39 33.75778335398948 38.0 32.5 40.0 21.5 41.0 40-41 33.478644776840184 38.0 31.5 40.0 19.5 41.0 42-43 33.163607189837954 37.0 31.0 40.0 17.5 41.0 44-45 32.91429495423742 37.0 31.0 40.0 16.5 41.0 46-47 32.48199185198034 37.0 30.5 40.0 14.5 41.0 48-49 32.12771626868752 37.0 30.0 39.5 11.0 41.0 50-51 31.53090168961012 36.0 30.0 38.5 9.0 40.0 52-53 31.59009646227115 36.0 30.0 39.0 8.5 40.0 54-55 31.627899612611067 36.0 30.0 39.0 8.0 40.0 56-57 31.475645515907928 36.0 30.0 39.0 7.5 40.0 58-59 31.064195088719586 36.0 29.0 39.0 4.5 40.0 60-61 30.624250109871543 35.0 28.0 39.0 2.0 40.0 62-63 30.19373895480183 35.0 28.0 38.0 2.0 40.0 64-65 29.561630670442593 34.0 27.0 38.0 2.0 40.0 66-67 29.006316977078114 34.0 26.0 38.0 2.0 39.5 68-69 28.470828393982124 34.0 26.0 37.0 2.0 39.0 70-71 27.901595541507177 33.0 25.5 36.0 2.0 39.0 72-73 27.1398075308217 32.5 23.5 36.0 2.0 38.5 74-75 26.66018731637522 32.0 22.0 35.0 2.0 38.0 76-77 26.25619617080823 32.0 22.0 35.0 2.0 37.5 78-79 25.65245062221595 31.0 20.0 35.0 2.0 37.0 80-81 24.959751065686554 31.0 18.0 34.5 2.0 36.0 82-83 24.268699058025653 30.5 14.5 34.0 2.0 36.0 84-85 23.588092261538307 30.0 7.5 34.0 2.0 35.0 86-87 22.89572613930124 29.5 3.5 34.0 2.0 35.0 88-89 22.061282257071547 29.0 2.0 34.0 2.0 35.0 90-91 20.79220737396079 28.0 2.0 33.5 2.0 35.0 92-93 19.113070324677523 25.0 2.0 32.0 2.0 34.0 94-95 18.84559026407816 25.0 2.0 32.0 2.0 34.0 96-97 18.188131087310353 24.5 2.0 32.0 2.0 34.0 98-99 17.170225246398353 21.5 2.0 32.0 2.0 34.0 100-101 15.54746106113591 8.5 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.94042742167969 1101 2 0.8061397828040704 1101 3 0.7376714489190412 1101 4 0.8167915683830955 1101 5 0.750706053519643 1101 6 0.5936444256421112 1101 7 0.8307599871960889 1101 8 0.8194522636714794 1101 9 1.017949164182383 1101 10-11 0.8374444387635265 1101 12-13 0.7584746440066112 1101 14-15 0.9668705156265887 1101 16-17 0.7303797724529275 1101 18-19 0.7156438518783403 1101 20-21 0.9541905153223667 1101 22-23 1.1050435301702848 1101 24-25 1.0369286075309319 1101 26-27 0.9997019358716059 1101 28-29 1.1534057564157152 1101 30-31 1.3438601484992958 1101 32-33 1.1201214456430435 1101 34-35 1.3069271794549309 1101 36-37 1.336586134611487 1101 38-39 1.206619726333038 1101 40-41 1.277343804502209 1101 42-43 1.1133481310965365 1101 44-45 1.2355397384340634 1101 46-47 1.2543086129115792 1101 48-49 1.0846574946360334 1101 50-51 1.1573834092782391 1101 52-53 1.1408284514086269 1101 54-55 1.3025542576499554 1101 56-57 0.9948312670160071 1101 58-59 0.9674642024967177 1101 60-61 1.0811568305557273 1101 62-63 1.194855232022828 1101 64-65 1.04494207739479 1101 66-67 1.011079433933606 1101 68-69 0.9451947992299701 1101 70-71 0.8827854227306204 1101 72-73 1.0488650302686437 1101 74-75 0.9129547001711487 1101 76-77 0.32907146225513983 1101 78-79 0.3787835788862495 1101 80-81 0.41416648401727585 1101 82-83 0.3705930581237027 1101 84-85 0.09976327849736677 1101 86-87 -0.2456557167229363 1101 88-89 -0.524874865588167 1101 90-91 -3.7329690979571986 1101 92-93 -9.138234828526219 1101 94-95 -7.262863382327009 1101 96-97 -3.8421120658687293 1101 98-99 -2.457313902107444 1101 100-101 -1.9208231209124325 1102 1 0.9787256858842746 1102 2 0.9616272594740103 1102 3 1.0441109563472608 1102 4 1.1085164386059176 1102 5 0.9765563874794339 1102 6 0.9856473759192994 1102 7 0.9347384515560577 1102 8 0.9634883933263083 1102 9 1.2073525069363669 1102 10-11 1.079672723811143 1102 12-13 1.1453328754918246 1102 14-15 1.2690032841530439 1102 16-17 1.4942117908290342 1102 18-19 1.2589996106041212 1102 20-21 1.3383518199121127 1102 22-23 1.3614545681153274 1102 24-25 1.4247891074956982 1102 26-27 1.2588202768335535 1102 28-29 1.2989186459363964 1102 30-31 1.3839286222539826 1102 32-33 1.2268726042018514 1102 34-35 1.3464755501228467 1102 36-37 1.14177899428352 1102 38-39 1.1977160413832095 1102 40-41 1.2866712891617667 1102 42-43 1.1303682127825496 1102 44-45 0.9300202471061212 1102 46-47 0.9508482412648434 1102 48-49 0.8782143987991518 1102 50-51 0.8616224439876561 1102 52-53 0.9838674574107777 1102 54-55 0.9898505992457025 1102 56-57 0.9595429427034183 1102 58-59 1.0017917628154827 1102 60-61 0.9114840069580055 1102 62-63 0.7094305481810856 1102 64-65 0.9020151118871773 1102 66-67 0.9823362805524773 1102 68-69 0.7769907372378348 1102 70-71 0.8006405876396165 1102 72-73 0.6491673212730227 1102 74-75 0.6974280666129502 1102 76-77 0.4889006972366765 1102 78-79 0.46824206640226507 1102 80-81 0.3590396640347713 1102 82-83 0.13468112891306205 1102 84-85 -0.10216577217831357 1102 86-87 -0.5233801727655809 1102 88-89 -1.1905635828098902 1102 90-91 -5.3738830959070505 1102 92-93 -10.338839950957965 1102 94-95 -9.004036653611134 1102 96-97 -5.566109505560766 1102 98-99 -4.068198864210821 1102 100-101 -3.306489287363579 1103 1 0.9188670202074611 1103 2 0.897363108122633 1103 3 0.8077495623686275 1103 4 1.0039025712966492 1103 5 0.986485948560599 1103 6 0.7338495637662987 1103 7 0.9144126330154592 1103 8 0.7750769989672648 1103 9 0.862625455244185 1103 10-11 0.7994832142487738 1103 12-13 0.7796701566240358 1103 14-15 0.9854006443853862 1103 16-17 0.9417960414379891 1103 18-19 0.9873907273959404 1103 20-21 1.1143956200979943 1103 22-23 1.1629169034551907 1103 24-25 1.043236488129736 1103 26-27 0.9854070745516097 1103 28-29 1.136160936836994 1103 30-31 1.2192057229783089 1103 32-33 1.1892371751595192 1103 34-35 1.10910878683341 1103 36-37 1.1145758610309926 1103 38-39 1.3163214901385132 1103 40-41 1.1092353683163552 1103 42-43 0.8964449119597973 1103 44-45 0.9618628148611847 1103 46-47 1.1798359926372015 1103 48-49 1.0735829928357674 1103 50-51 0.850841477351544 1103 52-53 1.1283344124641737 1103 54-55 1.19750906478966 1103 56-57 0.9355761516244279 1103 58-59 0.9699272717427796 1103 60-61 0.6258141685178416 1103 62-63 0.8724649515725353 1103 64-65 0.8108207175293423 1103 66-67 0.8641915217870597 1103 68-69 0.7308277382721329 1103 70-71 0.814700441700392 1103 72-73 0.9068971427111059 1103 74-75 0.6981946819850613 1103 76-77 0.5090353124133813 1103 78-79 0.5584197794178287 1103 80-81 0.5019454586379091 1103 82-83 0.45830533561931475 1103 84-85 0.24166252359707485 1103 86-87 -0.056322310022764555 1103 88-89 -0.3765487966833696 1103 90-91 -1.9030598282795985 1103 92-93 -8.727512543126997 1103 94-95 -5.310931166615166 1103 96-97 -2.838841683505228 1103 98-99 -1.868319372383315 1103 100-101 -1.408200967907005 1104 1 0.9746056419270701 1104 2 0.8740338409704407 1104 3 0.9453110938079412 1104 4 1.0134212168750452 1104 5 0.9896019345464993 1104 6 0.8470990966324337 1104 7 1.0038520945283835 1104 8 0.8931266527115866 1104 9 0.9881725882798591 1104 10-11 0.8969623693212014 1104 12-13 0.9247566357966548 1104 14-15 1.085904905358241 1104 16-17 1.056366486961636 1104 18-19 1.0174389032103193 1104 20-21 1.0640507357098983 1104 22-23 0.8664337413120009 1104 24-25 0.9197967169261005 1104 26-27 0.9171851745122055 1104 28-29 0.936017734692939 1104 30-31 1.0044106409622273 1104 32-33 1.0342907603405607 1104 34-35 1.0568187108876472 1104 36-37 1.0511323626366007 1104 38-39 0.8112938709395294 1104 40-41 0.7141985452729145 1104 42-43 0.8568365685035957 1104 44-45 0.7292351351549726 1104 46-47 0.551554237798122 1104 48-49 0.6132004072658148 1104 50-51 0.6252825988952679 1104 52-53 0.7939064297789109 1104 54-55 1.0017424584769081 1104 56-57 0.9650621399934742 1104 58-59 0.7585703869247276 1104 60-61 0.8186404591130056 1104 62-63 0.8387729272326823 1104 64-65 0.848027808117962 1104 66-67 0.8724753650794668 1104 68-69 0.9094023790998982 1104 70-71 0.9730092919216666 1104 72-73 0.8928749076674372 1104 74-75 0.8281809281741914 1104 76-77 0.5857915934878548 1104 78-79 0.4502485013746771 1104 80-81 0.4404322464450878 1104 82-83 0.35271868891049607 1104 84-85 0.3183241769536842 1104 86-87 0.2620810946671881 1104 88-89 -0.007797526534094601 1104 90-91 -0.3453316822146064 1104 92-93 -3.7281378441087227 1104 94-95 -1.388618776925444 1104 96-97 -0.41270206682328947 1104 98-99 -0.21379861194781924 1104 100-101 -0.26753013659867086 1105 1 0.9783094599461428 1105 2 0.9096181101405421 1105 3 0.9115429950524572 1105 4 0.8469069807306582 1105 5 0.6952621157411727 1105 6 0.7240283715775675 1105 7 0.9045735268013075 1105 8 0.9402561125227251 1105 9 1.0669908104147154 1105 10-11 0.9253932338215733 1105 12-13 0.8034939587484615 1105 14-15 0.9490496417942254 1105 16-17 1.1514449575447117 1105 18-19 1.0734258488762904 1105 20-21 0.9199559699520137 1105 22-23 1.0070553654362016 1105 24-25 1.0852079300727056 1105 26-27 0.9277239988099026 1105 28-29 1.023303567101678 1105 30-31 1.204078921822095 1105 32-33 1.2619415487827013 1105 34-35 1.1656654751639905 1105 36-37 1.154162670589571 1105 38-39 1.170908608886279 1105 40-41 1.1010249742887481 1105 42-43 1.2495501187918592 1105 44-45 1.0385177478412047 1105 46-47 1.1515865649817059 1105 48-49 1.0246247364767598 1105 50-51 0.9140000869269045 1105 52-53 0.8492785091640833 1105 54-55 1.1132119482759784 1105 56-57 1.051651069801256 1105 58-59 0.9740335022580418 1105 60-61 0.8058590379840211 1105 62-63 0.8878988749146295 1105 64-65 1.1013186016934604 1105 66-67 0.9544491426976833 1105 68-69 0.8086926430943997 1105 70-71 0.9259675541162444 1105 72-73 1.0770399484987827 1105 74-75 0.9816960626568978 1105 76-77 0.6982203396642284 1105 78-79 0.618878624786543 1105 80-81 0.5241515295288757 1105 82-83 0.49077769333070975 1105 84-85 0.46074735115821497 1105 86-87 0.3361168430090018 1105 88-89 -0.05818082296461924 1105 90-91 -1.1296443884222391 1105 92-93 -7.225925300186802 1105 94-95 -3.1547161710945346 1105 96-97 -1.1592171476981044 1105 98-99 -0.5612939050124197 1105 100-101 -0.2653955677929414 1106 1 0.9590850790482044 1106 2 1.0343265021676196 1106 3 0.8570514177716362 1106 4 0.9744817960324994 1106 5 0.93176578575153 1106 6 0.89710149054185 1106 7 0.9660239093716854 1106 8 0.9678292734552087 1106 9 1.095733397342947 1106 10-11 1.0401759171822746 1106 12-13 1.055812486355677 1106 14-15 1.0638216681744197 1106 16-17 1.1534009701979002 1106 18-19 1.063185759284572 1106 20-21 1.0307137655078975 1106 22-23 1.0073848691243796 1106 24-25 1.0390438188653945 1106 26-27 1.0084379901160432 1106 28-29 1.1690322949819674 1106 30-31 1.1411485383951074 1106 32-33 1.1416637202855924 1106 34-35 1.2202074447384135 1106 36-37 1.2583893610074384 1106 38-39 1.3079324105925494 1106 40-41 1.155211964916795 1106 42-43 1.172284320401836 1106 44-45 0.9963592911262538 1106 46-47 1.1447612764338047 1106 48-49 1.0765267689862341 1106 50-51 1.2260135894227133 1106 52-53 1.0829230127959342 1106 54-55 1.2462185250538873 1106 56-57 1.1419966371114008 1106 58-59 1.2881491702827539 1106 60-61 1.2151892447284283 1106 62-63 1.080192568712377 1106 64-65 1.1177129452929861 1106 66-67 1.1118219412319341 1106 68-69 1.046835965679069 1106 70-71 1.2886351567026217 1106 72-73 1.4588338370147902 1106 74-75 1.1741458558455022 1106 76-77 1.0305495993583023 1106 78-79 0.8822379364552155 1106 80-81 0.8507230256380112 1106 82-83 0.9190392140087518 1106 84-85 0.7779886100744307 1106 86-87 0.870098503055992 1106 88-89 0.5736601846295422 1106 90-91 0.06226281221531238 1106 92-93 -3.286369816038654 1106 94-95 -1.0015532350984309 1106 96-97 -0.23054995129492895 1106 98-99 0.07313461745203753 1106 100-101 0.10935466556956008 1107 1 0.5210141216820396 1107 2 0.49263484282523606 1107 3 0.4528560672988675 1107 4 0.43891947548901555 1107 5 0.45441841815489425 1107 6 0.5282572052068133 1107 7 0.4399681317823294 1107 8 0.5197518367948035 1107 9 0.4502631038485134 1107 10-11 0.34207599332565763 1107 12-13 0.386317916032084 1107 14-15 0.46967838761202785 1107 16-17 0.5752997323932831 1107 18-19 0.3838065629376999 1107 20-21 0.5215821748088558 1107 22-23 0.4892257997176088 1107 24-25 0.6204486618471137 1107 26-27 0.351818917455212 1107 28-29 0.43531145963306983 1107 30-31 0.6044042610013989 1107 32-33 0.5138005784053448 1107 34-35 0.3439177853998032 1107 36-37 0.5177863503172446 1107 38-39 0.4069968835955464 1107 40-41 0.5045262818511915 1107 42-43 0.3915779670060786 1107 44-45 0.23580017345525306 1107 46-47 0.29601801004456973 1107 48-49 0.30419529350115937 1107 50-51 0.17964513991812225 1107 52-53 0.4552647653023918 1107 54-55 0.41020425410767203 1107 56-57 0.17134046519850088 1107 58-59 0.29648041557845417 1107 60-61 0.2744193765575673 1107 62-63 0.11583788067613199 1107 64-65 0.07877759501303672 1107 66-67 -0.021586766863983087 1107 68-69 0.19165092512607984 1107 70-71 0.19808108113311818 1107 72-73 0.2372325816676799 1107 74-75 0.08127950015978058 1107 76-77 -0.2055581089941434 1107 78-79 -0.19846545509104985 1107 80-81 -0.21504379868585133 1107 82-83 -0.234209910034199 1107 84-85 -0.08295911616440677 1107 86-87 -0.2683192093605484 1107 88-89 -0.42299277636021415 1107 90-91 -0.7225634440039492 1107 92-93 -4.067309539320412 1107 94-95 -1.8015720398609645 1107 96-97 -1.1203602050787147 1107 98-99 -1.024888139292358 1107 100-101 -0.9930353780044161 1108 1 0.4395296784713061 1108 2 0.42938357097427016 1108 3 0.13292158260928844 1108 4 -0.07173377135662662 1108 5 -0.8271433837818236 1108 6 0.03056456042264699 1108 7 0.21100243962752074 1108 8 0.2120966236259605 1108 9 0.38849054746779643 1108 10-11 0.40242085252780413 1108 12-13 0.383766797700531 1108 14-15 0.42381518460380363 1108 16-17 0.556224840719544 1108 18-19 0.3479788453518253 1108 20-21 0.3523045832243028 1108 22-23 0.284433283204514 1108 24-25 0.3051485695659437 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80-81 0.8847392536336862 2311 82-83 0.9806914602555779 2311 84-85 0.9849055776707125 2311 86-87 1.1532694889749706 2311 88-89 1.2689648386377748 2311 90-91 2.115473235890896 2311 92-93 3.036723901840059 2311 94-95 2.417259742042173 2311 96-97 2.0455586079901806 2311 98-99 1.952029897974768 2311 100-101 1.9548375754754002 2312 1 0.3248057105404065 2312 2 0.08271194024420936 2312 3 0.10692406229205531 2312 4 0.15218095636958395 2312 5 0.3482883390104661 2312 6 0.40108601022668466 2312 7 0.4029331745007312 2312 8 -0.3935921617013989 2312 9 0.17056542417854814 2312 10-11 0.12484303359163107 2312 12-13 0.1889695511114624 2312 14-15 -0.20029822310434753 2312 16-17 0.22008716390904226 2312 18-19 0.2864609459492726 2312 20-21 0.3250805712510427 2312 22-23 0.20978578847100238 2312 24-25 0.43049641612940803 2312 26-27 0.41266972504858757 2312 28-29 0.26634310107650805 2312 30-31 0.4000163708597526 2312 32-33 0.27303795155740573 2312 34-35 0.14327819422839383 2312 36-37 0.3650475579975918 2312 38-39 0.4553445997652119 2312 40-41 0.45823278437470805 2312 42-43 0.5038271335662117 2312 44-45 0.26920638672904573 2312 46-47 0.42313500305804297 2312 48-49 0.2368538302710803 2312 50-51 0.44655828415389465 2312 52-53 0.2703685554973738 2312 54-55 0.43988789542845197 2312 56-57 0.4799121418234371 2312 58-59 0.7108885806805922 2312 60-61 0.6688963514745225 2312 62-63 0.6039864872554794 2312 64-65 0.6769949038794181 2312 66-67 0.6334818736941408 2312 68-69 0.4259011120053806 2312 70-71 0.47877514508187957 2312 72-73 0.5731852120958933 2312 74-75 0.7995176455206163 2312 76-77 0.8759557547219572 2312 78-79 0.8836623126043399 2312 80-81 0.7145724983839408 2312 82-83 0.9055627842017948 2312 84-85 0.7792174293184537 2312 86-87 0.650746581313129 2312 88-89 0.6508123012609026 2312 90-91 1.1308040274085975 2312 92-93 2.3539952585293484 2312 94-95 1.8024164841370443 2312 96-97 1.3564979844959488 2312 98-99 1.239672615696037 2312 100-101 1.3740968441288626 2313 1 -0.27087731089633493 2313 2 -0.9557878107475481 2313 3 -0.44753390697831463 2313 4 -0.3048284451635155 2313 5 -0.9723659733526091 2313 6 -0.47492178937239515 2313 7 -1.2874749368999758 2313 8 -1.3046503688496642 2313 9 -1.401008205335934 2313 10-11 -2.109680259030469 2313 12-13 -0.7047935047267799 2313 14-15 -0.35297943975367474 2313 16-17 -1.670126375377471 2313 18-19 -1.3540187909339139 2313 20-21 -1.573271339882453 2313 22-23 -1.2763576911788874 2313 24-25 -0.5899810642946761 2313 26-27 -1.0769618453215983 2313 28-29 -2.866512956965394 2313 30-31 -0.9479123782147809 2313 32-33 -0.5119700049301557 2313 34-35 -0.2720162300003537 2313 36-37 -0.35584827906325955 2313 38-39 -0.70642858962578 2313 40-41 -0.46890998756754954 2313 42-43 -0.24929386468522097 2313 44-45 -0.7039871828943092 2313 46-47 -0.9616969572161125 2313 48-49 -0.40369299258510694 2313 50-51 -0.34198703287670895 2313 52-53 -0.2956997316412284 2313 54-55 -0.13726477226463984 2313 56-57 -0.3440906950850966 2313 58-59 -0.27502066787673485 2313 60-61 -0.37855911099892836 2313 62-63 -0.2751678951945884 2313 64-65 -0.32011774015503747 2313 66-67 -0.2508723934257908 2313 68-69 -0.24746032450055466 2313 70-71 -0.29150907379769464 2313 72-73 -0.17843106221659966 2313 74-75 0.11824580031827026 2313 76-77 0.009744140643327626 2313 78-79 0.07723622854478052 2313 80-81 0.1701962288882619 2313 82-83 0.06897787049236115 2313 84-85 0.3042953132460404 2313 86-87 0.2025853865598144 2313 88-89 0.44036178058486186 2313 90-91 1.0597652242892117 2313 92-93 2.0410549593471856 2313 94-95 1.3121606647327901 2313 96-97 0.9023961103053217 2313 98-99 0.7098195476523976 2313 100-101 0.5605285731349614 2314 1 -0.5498636563320005 2314 2 -0.6815610201443256 2314 3 -1.82953048155672 2314 4 -0.5191167628167008 2314 5 -0.3612477032835386 2314 6 -2.8756290927244166 2314 7 -3.9866037586461402 2314 8 -1.4864346197487563 2314 9 -1.9537960992719974 2314 10-11 -1.2331665016058864 2314 12-13 -2.0061038405727345 2314 14-15 -2.894369251724534 2314 16-17 -2.1763221230784424 2314 18-19 -2.6556563885882483 2314 20-21 -2.1525847107538 2314 22-23 -3.2234390297681372 2314 24-25 -1.7198980166545965 2314 26-27 -1.7181258679944662 2314 28-29 -1.2328616591496768 2314 30-31 -1.327522694190172 2314 32-33 -2.1435758783188774 2314 34-35 -0.8054187798669901 2314 36-37 -0.43134587027299887 2314 38-39 -0.42284582464169773 2314 40-41 -0.6590316752807936 2314 42-43 -0.6168539388055976 2314 44-45 -1.3095834818164036 2314 46-47 -1.591277383483007 2314 48-49 -1.9241357613420043 2314 50-51 -1.0226125238266093 2314 52-53 -0.5550680584016199 2314 54-55 -0.2440497970936839 2314 56-57 -0.22487765146030014 2314 58-59 -0.3444701680704867 2314 60-61 -0.37541440104271473 2314 62-63 -0.450844008788291 2314 64-65 -0.45119955240466325 2314 66-67 -0.3552835501559848 2314 68-69 -0.3041503885057075 2314 70-71 -0.4243196854372009 2314 72-73 -0.3399693055648818 2314 74-75 0.051462507872329866 2314 76-77 -0.18729015215473055 2314 78-79 -0.14094937922508066 2314 80-81 -0.11530340427656327 2314 82-83 -0.09506108159275684 2314 84-85 -0.20770769219464924 2314 86-87 0.0017675294109658068 2314 88-89 0.30340118879303546 2314 90-91 0.8974131726275978 2314 92-93 1.8131728861421017 2314 94-95 0.9785021918620451 2314 96-97 0.8417679628313302 2314 98-99 0.859494245449909 2314 100-101 0.9231948556070417 2315 1 -7.066182266193522 2315 2 -2.971933307598551 2315 3 -1.4223190053379504 2315 4 -0.7685266776077455 2315 5 -1.175463326397761 2315 6 -1.5160254965030617 2315 7 -2.9440201386514353 2315 8 -3.074337618052116 2315 9 -3.3969606739518063 2315 10-11 -2.2174166569483873 2315 12-13 -2.596329483471301 2315 14-15 -4.38436398970013 2315 16-17 -4.0793904116722075 2315 18-19 -3.263411478584935 2315 20-21 -4.44441896347373 2315 22-23 -5.6687081052245745 2315 24-25 -3.342953686277326 2315 26-27 -4.3541668883019256 2315 28-29 -2.5826219790362686 2315 30-31 -2.5798755421257056 2315 32-33 -1.5871671620828849 2315 34-35 -2.2622446111849612 2315 36-37 -2.818844221062477 2315 38-39 -2.061415814664553 2315 40-41 -3.484762341203421 2315 42-43 -7.392518435466542 2315 44-45 -4.021455897345152 2315 46-47 -2.7106923532781195 2315 48-49 -5.4952654002583685 2315 50-51 -6.614772957172704 2315 52-53 -6.679541044873925 2315 54-55 -4.421867058078664 2315 56-57 -3.8182923345168014 2315 58-59 -4.354882286855666 2315 60-61 -3.141038025091305 2315 62-63 -3.250139799755839 2315 64-65 -1.9907715672100146 2315 66-67 -2.5489095947976033 2315 68-69 -1.8181285871012456 2315 70-71 -1.5575439159626896 2315 72-73 -1.9502263290388235 2315 74-75 -2.479417047386189 2315 76-77 -2.5846633730543935 2315 78-79 -1.6678482732729982 2315 80-81 -1.2582983767722133 2315 82-83 -1.6605343532587504 2315 84-85 -1.607195610395042 2315 86-87 -2.049471162875548 2315 88-89 -1.6087200682715341 2315 90-91 -0.7185314256401689 2315 92-93 0.11408930647001725 2315 94-95 0.07701892204069694 2315 96-97 0.0523541486422765 2315 98-99 -0.08844801874079877 2315 100-101 -0.1499309846406156 2316 1 -4.2593369469607545 2316 2 -2.9011703735425947 2316 3 -4.429413120329244 2316 4 -4.362869496199096 2316 5 -3.989910756091607 2316 6 -4.26914884981263 2316 7 -2.933293310164771 2316 8 -1.4805769712602128 2316 9 -1.8743007685699737 2316 10-11 -3.0237654603814335 2316 12-13 -3.8685118988002998 2316 14-15 -3.119614058658428 2316 16-17 -3.0645697562296093 2316 18-19 -3.266770205269566 2316 20-21 -5.557082452439278 2316 22-23 -6.51240324564743 2316 24-25 -4.346544093576021 2316 26-27 -3.9761565254512306 2316 28-29 -4.5990347764119015 2316 30-31 -6.208294276176588 2316 32-33 -4.395030077803483 2316 34-35 -5.936447239724352 2316 36-37 -9.369422684045439 2316 38-39 -6.331630951709336 2316 40-41 -5.4497103326786025 2316 42-43 -4.707606800026184 2316 44-45 -3.5222150005629587 2316 46-47 -5.827865907586272 2316 48-49 -3.355289448160221 2316 50-51 -4.440765483024435 2316 52-53 -5.331337685488965 2316 54-55 -7.020096828732516 2316 56-57 -4.978181259477008 2316 58-59 -5.7436795256979885 2316 60-61 -6.089803374534071 2316 62-63 -6.6702249195024415 2316 64-65 -5.467001906870173 2316 66-67 -6.763726734089513 2316 68-69 -5.386677791214915 2316 70-71 -6.192275264388822 2316 72-73 -8.295965733785017 2316 74-75 -8.068179461886732 2316 76-77 -7.423934425244028 2316 78-79 -6.344489087401566 2316 80-81 -6.372143228148214 2316 82-83 -5.539027022807055 2316 84-85 -6.824099159652139 2316 86-87 -5.576444132828357 2316 88-89 -5.39042009341442 2316 90-91 -6.051802945365454 2316 92-93 -4.6199224833919725 2316 94-95 -4.744470923990434 2316 96-97 -5.332382417815699 2316 98-99 -6.110752857124702 2316 100-101 -5.2543419555445485 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19709.0 3 7972.0 4 8157.0 5 9295.0 6 10527.0 7 12367.0 8 14749.0 9 17970.0 10 21782.0 11 25126.0 12 29685.0 13 32423.0 14 35506.0 15 38802.0 16 43309.0 17 47322.0 18 45538.0 19 45296.0 20 48159.0 21 52260.0 22 58154.0 23 64208.0 24 71383.0 25 80524.0 26 90493.0 27 101533.0 28 114901.0 29 129787.0 30 147096.0 31 168341.0 32 192953.0 33 224062.0 34 262359.0 35 312682.0 36 356496.0 37 305214.0 38 90527.0 39 1322.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.05082428744617 19.10678479769207 13.572863807785504 23.269527107076257 2 14.996525798309172 20.72156635065476 40.766255650263496 23.51565220077257 3 19.38234205748838 25.579552862813916 29.73704586223876 25.301059217458942 4 12.321583440592189 16.62544248026457 38.64179700666824 32.411177072475 5 13.309635134908202 38.171452287333956 34.642751757872944 13.876160819884895 6 30.782035737115486 37.090779565153255 18.238785528135274 13.888399169595989 7 27.564554478069127 33.12923430027237 21.80619098067991 17.500020240978596 8 25.471887926523745 35.05906376547809 20.479136899898197 18.98991140809996 9 26.56928756299578 16.76023583989177 20.550327062339736 36.12014953477271 10-11 24.48795669345289 26.511991431286418 28.354552713810783 20.645499161449905 12-13 26.075570525623913 24.01245175716225 28.17398216645462 21.73799555075922 14-15 22.971193796957223 25.243042920302432 26.726782108586534 25.05898117415381 16-17 22.07982914830237 28.444081873035504 27.113261217540245 22.362827761121885 18-19 21.925878079579604 27.740753501987708 28.889760518707703 21.443607899724988 20-21 23.098859686189527 26.737019457281043 28.489986153567937 21.674134702961492 22-23 22.54521934761529 26.647270343120272 28.398038232982024 22.40947207628241 24-25 22.504564128879508 26.704357698704044 28.658484359711707 22.13259381270474 26-27 22.456941657626174 26.672485983237486 28.85798487437717 22.012587484759173 28-29 22.137655011482277 27.184741420394126 28.73074230453466 21.946861263588936 30-31 22.35472717044815 26.969420588786797 28.560345021563847 22.1155072192012 32-33 22.2232372442482 26.994510780883946 28.50937865351678 22.27287332135107 34-35 22.64179329471931 26.895190896429867 28.398392940578248 22.064622868272576 36-37 22.750612345368637 26.640792334181494 28.58684234092408 22.02175297952579 38-39 22.50257797869971 26.936941328579607 28.55401455967374 22.00646613304695 40-41 22.578522635321857 26.8820797483856 28.619592956416685 21.919804659875858 42-43 22.304644033615258 27.034349137413198 28.2605073185979 22.400499510373646 44-45 22.45044318698707 27.05588956610822 28.113005595570336 22.380661651334375 46-47 22.71279709829059 27.089436123558148 27.69531391210422 22.50245286604704 48-49 22.607458531541592 27.055719949807578 27.873897239956865 22.462924278693965 50-51 22.45917125387402 27.28499641105539 27.979154113362636 22.276678221707954 52-53 22.61784702128748 27.437919949750455 27.59612018536272 22.348112843599345 54-55 22.371983064015897 27.145799329174658 27.758766958566017 22.723450648243425 56-57 22.458256813095016 27.50468496612259 27.539275638911175 22.497782581871217 58-59 22.724540165319997 27.14261287192125 27.556932805565264 22.575914157193488 60-61 22.455738707112193 27.118814135416564 27.98449696115601 22.44095019631523 62-63 22.520725332261478 27.044040815500107 27.95710278040034 22.47813107183807 64-65 22.42472792643753 27.280835940987707 27.826190232329296 22.468245900245467 66-67 22.324492551543482 27.265143815119718 27.566315612025456 22.84404802131134 68-69 22.291706222904914 27.34316157752228 27.719002081970423 22.646130117602382 70-71 22.50154524419508 27.101790833405953 27.52000043196343 22.876663490435533 72-73 22.41482959768343 26.99398818340699 27.59429967385171 22.99688254505787 74-75 22.207774123906066 27.575944546511437 27.33618047231977 22.880100857262725 76-77 22.46253655409526 27.035714971693086 27.465195579415425 23.036552894796234 78-79 22.38622950996783 27.212500326363998 27.49671460241062 22.90455556125755 80-81 22.435597695760976 27.33482175281352 27.304959324070676 22.924621227354823 82-83 22.436168605189465 27.256947596783455 27.401412051129643 22.905471746897437 84-85 22.38996503635514 27.183997587576016 27.24949909946673 23.176538276602113 86-87 22.28671912835109 27.147091353063775 27.461084316164765 23.10510520242037 88-89 22.495538787786753 27.013877926348133 27.239354464243377 23.251228821621737 90-91 22.13760028548076 26.98252782040665 27.65280985581479 23.227062038297795 92-93 21.89734655927367 27.108750167914504 27.951635803528884 23.04226746928294 94-95 22.32031078490174 27.164687997390217 27.23829068304043 23.276710534667615 96-97 22.16138257167877 26.957911584988377 27.31974784020074 23.56095800313211 98-99 22.33682252838677 26.978029854420488 26.954778561076676 23.730369056116075 100-101 22.440319775667657 26.942953924352388 27.091553085658056 23.525173214321903 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3175.0 1 1886.5 2 622.0 3 617.5 4 543.5 5 558.5 6 780.5 7 1300.0 8 1979.5 9 2300.5 10 2097.0 11 1897.5 12 1978.0 13 2130.0 14 2326.5 15 2554.0 16 2857.5 17 3231.5 18 3601.5 19 4023.0 20 4637.5 21 5376.5 22 6536.5 23 8368.5 24 10673.0 25 13606.5 26 17846.5 27 23063.5 28 27775.5 29 33309.5 30 39324.0 31 45453.5 32 52042.0 33 58168.0 34 65580.5 35 73242.5 36 80996.5 37 88177.5 38 92964.5 39 96119.5 40 99609.5 41 103833.0 42 107198.5 43 111017.0 44 114996.0 45 117969.5 46 121947.5 47 131106.0 48 147871.0 49 148405.5 50 132278.0 51 123328.0 52 117420.0 53 112187.5 54 107375.0 55 100417.0 56 92157.5 57 83230.5 58 75026.0 59 66588.5 60 56411.5 61 46201.0 62 36806.5 63 28228.5 64 21056.0 65 15503.5 66 11052.0 67 7953.0 68 5641.0 69 3936.0 70 2863.5 71 1993.5 72 1372.5 73 897.5 74 596.0 75 435.0 76 302.0 77 186.0 78 123.0 79 84.0 80 55.0 81 39.0 82 30.5 83 25.5 84 22.0 85 14.5 86 9.5 87 10.0 88 8.0 89 6.5 90 5.0 91 4.5 92 5.0 93 3.5 94 1.0 95 1.0 96 1.0 97 2.0 98 1.5 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09131246388169643 2 0.10215731687551997 3 0.09775346773161925 4 0.1011686976799504 5 0.09817288193580027 6 0.0822351421769215 7 0.09496735908955961 8 0.09089304967751542 9 0.08523095792107163 10-11 0.09185171071564346 12-13 0.09101288230728141 14-15 0.09701949287430246 16-17 0.09487748461723511 18-19 0.09706443011046471 20-21 0.10653120786197917 22-23 0.1153988224646636 24-25 0.13956007644123453 26-27 0.14490760754454254 28-29 0.14405380005745977 30-31 0.14571647779546307 32-33 0.1416421683834189 34-35 0.1369537167438239 36-37 0.1407584027388946 38-39 0.13380811021246625 40-41 0.12931438659624103 42-43 0.12955405185577304 44-45 0.12020710673402459 46-47 0.139110704079612 48-49 0.15623179105743007 50-51 0.16575848512382754 52-53 0.1743414972308177 54-55 0.17004250163796228 56-57 0.1414174822026076 58-59 0.13343363324444746 60-61 0.12941924014728629 62-63 0.12601898927767585 64-65 0.11187873896528717 66-67 0.12515020271187233 68-69 0.12366727391851802 70-71 0.1310220015704066 72-73 0.15885312983356148 74-75 0.17240919607584088 76-77 0.17798141335996015 78-79 0.17435647630953846 80-81 0.1810371454189933 82-83 0.18057279397865 84-85 0.15702568222962987 86-87 0.15790944787415417 88-89 0.17780166441531114 90-91 0.26566894019123494 92-93 0.5346632358584765 94-95 0.17703773140055284 96-97 0.1908784001385265 98-99 0.20945245775225743 100-101 0.21619304317659527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3337989.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.53537111347027 #Duplication Level Percentage of deduplicated Percentage of total 1 74.39596948005415 41.316077744212144 2 13.475357737840653 14.967179857155077 3 5.0841066010823015 8.470432406146484 4 2.42500106447702 5.3869333626516696 5 1.33734648525498 3.71350166829652 6 0.836377342594965 2.7869115667145663 7 0.5388701091983561 2.094844604740087 8 0.3754885787950101 1.6682318057800325 9 0.2675140639103131 1.3370843535598658 >10 1.1804600516054415 11.177307541634764 >50 0.05494265650647732 2.110018199669312 >100 0.02594046285755062 2.5870285109040614 >500 0.0014767682763133765 0.5880930585783553 >1k 9.845121826284333E-4 1.1514607529537357 >5k 1.6408536377140553E-4 0.6448945670032912 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9248 0.27705304001900544 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6263 0.1876279400561236 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5720 0.17136066056538832 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4413 0.13220534878934592 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4273 0.12801120674753572 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3506 0.10503329998990409 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.991631488300291E-5 2 0.0 0.0 0.0 0.0 5.991631488300291E-5 3 0.0 0.0 0.0 0.0 5.991631488300291E-5 4 0.0 0.0 0.0 0.0 5.991631488300291E-5 5 0.0 0.0 0.0 0.0 5.991631488300291E-5 6 0.0 0.0 0.0 0.0 5.991631488300291E-5 7 0.0 0.0 0.0 0.0 5.991631488300291E-5 8 0.0 0.0 0.0 0.0 5.991631488300291E-5 9 0.0 0.0 0.0 0.0 8.987447232450436E-5 10-11 0.0 0.0 0.0 1.4979078720750728E-5 8.987447232450436E-5 12-13 1.4979078720750728E-5 0.0 0.0 2.9958157441501456E-5 1.1983262976600582E-4 14-15 2.9958157441501456E-5 0.0 0.0 5.991631488300291E-5 1.9472802336975947E-4 16-17 4.493723616225218E-5 0.0 0.0 1.9472802336975947E-4 2.3966525953201165E-4 18-19 7.489539360375364E-5 1.4979078720750728E-5 0.0 2.546443382527624E-4 2.3966525953201165E-4 20-21 8.987447232450436E-5 2.9958157441501456E-5 0.0 4.194142041810204E-4 2.9958157441501457E-4 22-23 8.987447232450436E-5 2.9958157441501456E-5 0.0 6.441003849922813E-4 2.9958157441501457E-4 24-25 1.7974894464900873E-4 2.9958157441501456E-5 0.0 0.0010185773530110495 3.29539731856516E-4 26-27 1.7974894464900873E-4 2.9958157441501456E-5 0.0 0.0015128869507958233 3.29539731856516E-4 28-29 2.546443382527624E-4 2.9958157441501456E-5 0.0 0.002920920350546392 3.29539731856516E-4 30-31 2.9958157441501457E-4 2.9958157441501456E-5 0.0 0.008118660666646894 3.445188105772667E-4 32-33 2.9958157441501457E-4 2.9958157441501456E-5 0.0 0.020101923643247477 3.5949788929801746E-4 34-35 3.2953973185651604E-4 2.9958157441501456E-5 0.0 0.038765855729302884 3.5949788929801746E-4 36-37 3.5949788929801746E-4 2.9958157441501456E-5 0.0 0.06469464099492239 3.5949788929801746E-4 38-39 4.0443512546026964E-4 2.9958157441501456E-5 0.0 0.10632150075988867 3.5949788929801746E-4 40-41 4.1941420418102035E-4 2.9958157441501456E-5 0.0 0.15377522214722697 3.5949788929801746E-4 42-43 4.493723616225218E-4 2.9958157441501456E-5 0.0 0.2018880229982783 3.5949788929801746E-4 44-45 4.793305190640233E-4 2.9958157441501456E-5 0.0 0.25883848029457257 4.194142041810204E-4 46-47 5.392468339470262E-4 2.9958157441501456E-5 0.0 0.32922517120338024 4.793305190640233E-4 48-49 5.692049913885276E-4 2.9958157441501456E-5 0.0 0.4055585563643259 4.793305190640233E-4 50-51 5.692049913885276E-4 2.9958157441501456E-5 0.0 0.4891867528622773 5.092886765055248E-4 52-53 5.692049913885276E-4 2.9958157441501456E-5 0.0 0.5708377109690894 5.542259126677768E-4 54-55 5.692049913885276E-4 2.9958157441501456E-5 0.0 0.6605174552702241 5.692049913885276E-4 56-57 5.692049913885276E-4 2.9958157441501456E-5 0.0 0.7669737677385995 5.841840701092784E-4 58-59 5.692049913885276E-4 2.9958157441501456E-5 0.0 0.8850688243729983 5.991631488300291E-4 60-61 5.841840701092784E-4 2.9958157441501456E-5 0.0 1.0242244656887725 5.991631488300291E-4 62-63 5.991631488300291E-4 2.9958157441501456E-5 0.0 1.1659265503870744 6.291213062715306E-4 64-65 5.991631488300291E-4 2.9958157441501456E-5 0.0 1.3097856224211644 6.291213062715306E-4 66-67 6.291213062715306E-4 2.9958157441501456E-5 0.0 1.4730575804773474 6.441003849922812E-4 68-69 6.59079463713032E-4 2.9958157441501456E-5 0.0 1.658888031086981 6.59079463713032E-4 70-71 6.740585424337827E-4 2.9958157441501456E-5 0.0 1.8673069324075064 6.59079463713032E-4 72-73 6.890376211545335E-4 2.9958157441501456E-5 0.0 2.0875593059174253 7.189957785960349E-4 74-75 6.890376211545335E-4 2.9958157441501456E-5 0.0 2.3153311769451608 7.789120934790379E-4 76-77 6.890376211545335E-4 2.9958157441501456E-5 1.4979078720750728E-5 2.5540227963603237 7.789120934790379E-4 78-79 6.890376211545335E-4 2.9958157441501456E-5 2.9958157441501456E-5 2.8297277192944614 7.938911721997886E-4 80-81 6.890376211545335E-4 5.991631488300291E-5 2.9958157441501456E-5 3.1350462808595236 8.238493296412899E-4 82-83 7.040166998752843E-4 5.991631488300291E-5 2.9958157441501456E-5 3.4396758048034313 8.388284083620407E-4 84-85 7.639330147582871E-4 5.991631488300291E-5 2.9958157441501456E-5 3.7485144498678693 8.538074870827915E-4 86-87 8.088702509205393E-4 5.991631488300291E-5 2.9958157441501456E-5 4.082173428372592 8.83765644524293E-4 88-89 8.088702509205393E-4 5.991631488300291E-5 2.9958157441501456E-5 4.435469979080219 8.987447232450436E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13165 0.0 27.328588 1 TACATGG 12945 0.0 27.16971 2 ACATGGG 13290 0.0 25.107254 3 GAGTACT 8270 0.0 21.949423 12-13 CATGGGG 5985 0.0 21.095015 4 GTACTTT 9115 0.0 20.070961 14-15 CATGGGA 7940 0.0 19.906096 4 GTATCAA 25145 0.0 19.839008 1 AGTACTT 9055 0.0 19.39146 12-13 GGACCGT 330 6.0754246E-10 18.69365 6 TATCAAC 27820 0.0 17.880226 2 TCAACGC 27690 0.0 17.826773 4 ATCAACG 27740 0.0 17.794907 3 CAACGCA 27745 0.0 17.739845 5 AACGCAG 28290 0.0 17.411215 6 GTATTAG 1020 0.0 17.217514 1 AGTACAT 9835 0.0 16.794756 2 ACTTTTT 11830 0.0 16.40971 16-17 ACGCAGA 29670 0.0 16.31375 7 GGACCGA 525 0.0 16.269636 6 >>END_MODULE