FastQCFastQC Report
Fri 27 May 2016
SRR939609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939609_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337989
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97390.29176249532278264No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70310.21063580497119672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66890.20039011512620322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51610.154614050555589No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA47060.14098308891970585No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA40190.12040183475739434No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36740.11006627044007634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG123550.023.5826972
GTACATG125650.023.5652921
ACATGGG124200.022.244853
GTATCAA221600.019.840921
GAGTACT83200.019.40255212-13
CATGGGA76750.018.887974
AGTACTT91250.018.411912-13
TCAACGC246450.017.6082044
AACGCAG247650.017.553046
ATCAACG247800.017.5312713
CAACGCA248800.017.441895
GTACTTT92250.017.21865714-15
TATCAAC256900.016.9115412
GGTATCA175400.016.2640131
CGCAGAG264600.016.091648
ACGCAGA267300.016.0868517
GTGTAGC21250.015.949911
CATGGGG55250.015.5894864
ATGGGAT33250.015.4293585
AGAGTAC194900.015.27531710-11
Produced by FastQC (version 0.11.5)