##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939609_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3337989 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.5577465953303 31.0 31.0 34.0 30.0 34.0 2 31.769809007758862 31.0 31.0 34.0 30.0 34.0 3 31.81956171814826 31.0 31.0 34.0 30.0 34.0 4 35.59605439083232 37.0 35.0 37.0 33.0 37.0 5 35.33232134677496 37.0 35.0 37.0 33.0 37.0 6 35.35064675168193 37.0 35.0 37.0 32.0 37.0 7 35.28113304148096 37.0 35.0 37.0 32.0 37.0 8 35.245414829108185 37.0 35.0 37.0 32.0 37.0 9 36.862312607980435 39.0 37.0 39.0 33.0 39.0 10-11 36.74680608593977 39.0 35.0 39.0 32.0 39.0 12-13 36.69015356251923 39.0 35.0 39.0 32.0 39.0 14-15 37.75561138757497 40.0 37.0 41.0 32.0 41.0 16-17 37.720294015348756 39.0 37.0 41.0 32.0 41.0 18-19 37.70358620115285 39.5 37.0 41.0 32.0 41.0 20-21 37.63945267644681 39.0 36.5 41.0 32.0 41.0 22-23 37.50504255706055 39.0 36.0 41.0 32.0 41.0 24-25 37.3764402758667 39.0 36.0 41.0 31.5 41.0 26-27 37.11717459224701 39.0 36.0 40.5 31.0 41.0 28-29 36.91899374144133 39.0 36.0 40.0 30.5 41.0 30-31 36.69799151525065 38.5 35.5 40.0 30.0 41.0 32-33 36.545595866253606 38.0 35.0 40.0 30.0 41.0 34-35 36.5410655936853 38.0 35.0 40.0 30.0 41.0 36-37 36.445297003674966 38.5 35.0 40.0 30.0 41.0 38-39 36.479232406098404 39.0 35.0 40.0 30.0 41.0 40-41 36.39606421710796 39.0 35.0 40.0 30.0 41.0 42-43 36.22431365112348 38.5 35.0 40.0 30.0 41.0 44-45 36.03405149028352 38.0 35.0 40.0 29.0 41.0 46-47 35.7632865177207 38.0 35.0 40.0 28.5 41.0 48-49 35.48598078064367 38.0 34.0 40.0 27.0 41.0 50-51 35.249233895018826 38.0 34.0 40.0 27.0 41.0 52-53 35.01028778105619 38.0 34.0 40.0 26.5 41.0 54-55 34.72730317565457 38.0 34.0 40.0 26.0 41.0 56-57 32.68177306755654 37.0 30.5 39.5 16.5 41.0 58-59 27.575051924976385 32.5 17.5 38.5 7.0 40.0 60-61 28.330917956889614 33.0 20.0 38.0 9.5 40.0 62-63 31.468726230074452 35.0 29.5 38.5 14.5 40.0 64-65 31.716868150254538 35.5 31.0 39.0 10.0 40.0 66-67 24.958692793774937 27.5 18.0 35.5 2.0 39.0 68-69 17.397576954267976 13.0 7.5 28.0 2.0 35.0 70-71 16.24347833381117 12.5 7.5 26.5 2.0 33.5 72-73 17.35282366119241 13.5 7.0 28.5 2.0 34.0 74-75 18.372229207465935 17.5 8.5 29.5 2.0 34.5 76-77 19.5926671717612 21.5 8.0 30.0 2.0 34.5 78-79 22.690485498903683 27.5 10.5 33.5 2.0 35.5 80-81 26.382935803563164 31.5 23.5 34.5 2.0 36.0 82-83 27.71284087514968 33.5 26.5 35.0 2.0 36.0 84-85 27.771387053702092 34.0 27.0 35.0 2.0 36.0 86-87 27.540533536809136 34.0 27.0 35.0 2.0 36.0 88-89 27.11171876240455 33.0 26.0 35.0 2.0 35.5 90-91 25.73621512833026 32.5 21.0 35.0 2.0 35.0 92-93 23.23411146651472 31.5 2.0 34.5 2.0 35.0 94-95 15.80258458011695 4.5 2.0 32.0 2.0 35.0 96-97 13.209588617577829 2.0 2.0 30.0 2.0 34.0 98-99 12.873530140452829 2.0 2.0 29.5 2.0 34.0 100-101 12.582903508669443 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2632009770395243 1101 2 0.24804653483913341 1101 3 0.3288899571417474 1101 4 0.19763992789254559 1101 5 0.36861516213287615 1101 6 0.33594881979846747 1101 7 0.3612090378475017 1101 8 0.31661437670787507 1101 9 0.42084477441187573 1101 10-11 0.4780351257190816 1101 12-13 0.4349084831266765 1101 14-15 0.4689292440467483 1101 16-17 0.6426657527220527 1101 18-19 0.4591829750526273 1101 20-21 0.3793303752175845 1101 22-23 0.48683479048264644 1101 24-25 0.4514583099309277 1101 26-27 0.522591173982228 1101 28-29 0.5934288364100482 1101 30-31 0.6004271808886372 1101 32-33 0.6243313311595955 1101 34-35 0.5456718448853835 1101 36-37 0.4042366535123989 1101 38-39 0.5296699626325889 1101 40-41 0.5826716054966639 1101 42-43 0.6463502390681057 1101 44-45 0.684957563262742 1101 46-47 0.6045960091896134 1101 48-49 0.6439809409322237 1101 50-51 0.46719993678063076 1101 52-53 0.5025108070374742 1101 54-55 0.5724281357297158 1101 56-57 -1.7212423051143446 1101 58-59 -9.602729430333621 1101 60-61 -9.98436748365518 1101 62-63 -3.6455505011401073 1101 64-65 -5.248394501468027 1101 66-67 -9.855541075772086 1101 68-69 -4.929717254265395 1101 70-71 -4.666844139367861 1101 72-73 -4.834491051551797 1101 74-75 -6.80669100125078 1101 76-77 -8.043510070475605 1101 78-79 -11.004455129975241 1101 80-81 -7.839789626857101 1101 82-83 -4.193197332510369 1101 84-85 -3.484335177606482 1101 86-87 -3.253737301716008 1101 88-89 -3.297460840355619 1101 90-91 -10.078842921817857 1101 92-93 -17.06273612167805 1101 94-95 -11.039915048610673 1101 96-97 -9.174133737618792 1101 98-99 -9.265112605658306 1101 100-101 -9.038649853908261 1102 1 0.33710187556840765 1102 2 0.36831702303132374 1102 3 0.3293711497731451 1102 4 0.33495038533683186 1102 5 0.25133126755945767 1102 6 0.26569556029124186 1102 7 0.38440982919969713 1102 8 0.4264488646008857 1102 9 0.5249633164111742 1102 10-11 0.4597506704027694 1102 12-13 0.39279656195753176 1102 14-15 0.4953189633856283 1102 16-17 0.45411054981051535 1102 18-19 0.5517211170265028 1102 20-21 0.6252449935730269 1102 22-23 0.5789937716858731 1102 24-25 0.5564802230023744 1102 26-27 0.5215547507696314 1102 28-29 0.530621308706877 1102 30-31 0.3919903565524834 1102 32-33 0.38491778521953535 1102 34-35 0.35205374322273997 1102 36-37 0.3782714904754272 1102 38-39 0.39003698334116876 1102 40-41 0.2510743269803797 1102 42-43 0.23394513194113387 1102 44-45 0.3422273549577781 1102 46-47 0.2615357105216063 1102 48-49 0.35112950494186634 1102 50-51 0.2716984609935196 1102 52-53 0.10425991847399274 1102 54-55 -0.043181528416859294 1102 56-57 -2.817011419983629 1102 58-59 -10.373884720663984 1102 60-61 -10.77999312702875 1102 62-63 -4.592399143359767 1102 64-65 -5.629071807117867 1102 66-67 -7.2081653482006125 1102 68-69 -4.297803676980799 1102 70-71 -4.150914631278161 1102 72-73 -4.753096863995619 1102 74-75 -5.367059599994491 1102 76-77 -7.564961836858961 1102 78-79 -9.92792215530984 1102 80-81 -5.616092013840603 1102 82-83 -3.1692338962329423 1102 84-85 -2.703859860453843 1102 86-87 -2.6908402799654922 1102 88-89 -2.8380567669497445 1102 90-91 -8.76962931509102 1102 92-93 -13.308532819736808 1102 94-95 -10.464957947387717 1102 96-97 -9.137371895978294 1102 98-99 -9.236172305969976 1102 100-101 -9.093049480713761 1103 1 0.3113340689292663 1103 2 0.244107513797303 1103 3 0.25915366645789106 1103 4 0.23258310750012612 1103 5 0.22002897444049552 1103 6 0.25928714429998934 1103 7 0.25267892020489313 1103 8 0.24990168902494503 1103 9 0.41132264819646025 1103 10-11 0.3874287488069541 1103 12-13 0.3569534392121767 1103 14-15 0.47503258679549276 1103 16-17 0.42770536525623015 1103 18-19 0.5064395555577264 1103 20-21 0.3438016504332708 1103 22-23 0.48627675786997315 1103 24-25 0.4804737825523162 1103 26-27 0.5697477087997953 1103 28-29 0.4367450619308144 1103 30-31 0.6645342342207741 1103 32-33 0.48985769931216083 1103 34-35 0.4637344267977923 1103 36-37 0.33278791414364406 1103 38-39 0.48792840065262766 1103 40-41 0.5245881362679441 1103 42-43 0.3724701671686077 1103 44-45 0.3748968021818513 1103 46-47 0.4460169247410448 1103 48-49 0.5491751462860037 1103 50-51 0.7188226222514373 1103 52-53 0.6852839956825179 1103 54-55 0.5935388874174308 1103 56-57 -2.522539619776932 1103 58-59 -10.300882976008367 1103 60-61 -10.41411567977585 1103 62-63 -4.05510197409053 1103 64-65 -5.217116050975637 1103 66-67 -6.728528906881628 1103 68-69 -4.2908160449353545 1103 70-71 -4.181322973591044 1103 72-73 -4.6378284645259455 1103 74-75 -5.100368447284469 1103 76-77 -6.540040430451107 1103 78-79 -9.2545590663284 1103 80-81 -4.990070477334374 1103 82-83 -2.84517062253504 1103 84-85 -2.400183970777583 1103 86-87 -2.191698692973567 1103 88-89 -2.159693760055614 1103 90-91 -5.27790271467882 1103 92-93 -10.392551181666555 1103 94-95 -8.601058804259086 1103 96-97 -8.031627826586089 1103 98-99 -8.1562353850609 1103 100-101 -8.390710072519205 1104 1 0.22811141851165218 1104 2 0.21394188377686163 1104 3 0.13166568571118376 1104 4 0.1575373650915921 1104 5 0.20718524202349187 1104 6 0.19685192936560725 1104 7 0.23235150659760961 1104 8 0.21064881701834537 1104 9 0.1751051199405893 1104 10-11 0.25607661837163676 1104 12-13 0.31908327321080066 1104 14-15 0.38276170383797137 1104 16-17 0.3623848140749004 1104 18-19 0.3522634069552453 1104 20-21 0.29501365178309413 1104 22-23 0.35953251180588097 1104 24-25 0.3538239086856976 1104 26-27 0.42024264327840655 1104 28-29 0.4144519299611744 1104 30-31 0.38510599345992347 1104 32-33 0.3198930309682595 1104 34-35 0.4337248345623337 1104 36-37 0.22885286606408073 1104 38-39 0.29271180197992663 1104 40-41 0.1927687186602398 1104 42-43 0.33484938906813255 1104 44-45 0.37684365462205704 1104 46-47 0.2578235169286316 1104 48-49 0.2933988523435289 1104 50-51 0.31549705844143716 1104 52-53 0.32113434965490484 1104 54-55 0.11704701884280411 1104 56-57 -3.1006458576599414 1104 58-59 -10.553348321907173 1104 60-61 -10.616711366625733 1104 62-63 -4.419377123216492 1104 64-65 -5.5630821846438 1104 66-67 -6.766644670564787 1104 68-69 -4.162578622883981 1104 70-71 -4.1164191761662465 1104 72-73 -4.341203871846741 1104 74-75 -5.199568106318061 1104 76-77 -6.8380912953620765 1104 78-79 -9.321937706493173 1104 80-81 -5.258778274947915 1104 82-83 -2.8945164647839015 1104 84-85 -2.3262178020179505 1104 86-87 -2.094802848365397 1104 88-89 -2.0602785695728265 1104 90-91 -4.6945055438346515 1104 92-93 -7.034395064499895 1104 94-95 -7.635144841040734 1104 96-97 -7.9176774438761655 1104 98-99 -8.064827948088835 1104 100-101 -8.220763290602623 1105 1 0.29150031453021086 1105 2 0.3189045125779444 1105 3 0.29539718489650824 1105 4 0.18317493350257763 1105 5 0.281295716518315 1105 6 0.18053726078422017 1105 7 0.2518647794244231 1105 8 0.23542216122487503 1105 9 0.30189103438564047 1105 10-11 0.37373700749995464 1105 12-13 0.3717733334953124 1105 14-15 0.4848197595609349 1105 16-17 0.43010214128284474 1105 18-19 0.4463013970816476 1105 20-21 0.5136477551748513 1105 22-23 0.5085332119574488 1105 24-25 0.4276850252568707 1105 26-27 0.5445130479855109 1105 28-29 0.6080259681462366 1105 30-31 0.6564236592098354 1105 32-33 0.6844429439772668 1105 34-35 0.6176202114501947 1105 36-37 0.4147829061400401 1105 38-39 0.4392432901187746 1105 40-41 0.36427823568561024 1105 42-43 0.3579145908856063 1105 44-45 0.335972510126048 1105 46-47 0.36926376605912736 1105 48-49 0.30802243559251963 1105 50-51 0.3592281027719224 1105 52-53 0.4337466622506483 1105 54-55 0.2987729005708033 1105 56-57 1.5973768233519792 1105 58-59 -8.512862179547351 1105 60-61 -9.923631170843212 1105 62-63 -3.6588167745377618 1105 64-65 -5.003107136680985 1105 66-67 -6.362294958666595 1105 68-69 -3.808688558050921 1105 70-71 -3.728557952379287 1105 72-73 -2.343364432357866 1105 74-75 -4.664215325774151 1105 76-77 -6.553525170026823 1105 78-79 -9.139401208453108 1105 80-81 -4.519877951513632 1105 82-83 -2.1952193779667333 1105 84-85 -1.7495024712978662 1105 86-87 -1.526516952601881 1105 88-89 -1.6569011099046485 1105 90-91 -4.52475290364595 1105 92-93 -6.772846218775289 1105 94-95 -8.157655282396137 1105 96-97 -8.17681312220282 1105 98-99 -8.507673812402388 1105 100-101 -8.43203686792226 1106 1 0.23544581493482042 1106 2 0.2687174419717948 1106 3 0.211268443294081 1106 4 0.1956421797804211 1106 5 0.22763244036154617 1106 6 0.3386727438910313 1106 7 0.2673753548536695 1106 8 0.29914095822025644 1106 9 0.22286597150184662 1106 10-11 0.3760131862964755 1106 12-13 0.32999111894024935 1106 14-15 0.38966449395193337 1106 16-17 0.35541020667854895 1106 18-19 0.3886002417533092 1106 20-21 0.4260696027156783 1106 22-23 0.43129684713267125 1106 24-25 0.47614916659806994 1106 26-27 0.5895816723428737 1106 28-29 0.5476855354707979 1106 30-31 0.5283598914922223 1106 32-33 0.4788793302783958 1106 34-35 0.6351316603458557 1106 36-37 0.5378989930348226 1106 38-39 0.5886862801955033 1106 40-41 0.5945245834622668 1106 42-43 0.48155337730113246 1106 44-45 0.4647976429805354 1106 46-47 0.46890841158106156 1106 48-49 0.5750265360941427 1106 50-51 0.4513932733845962 1106 52-53 0.5220682152223972 1106 54-55 0.35328914114939636 1106 56-57 1.6245919097389887 1106 58-59 -8.535373223985236 1106 60-61 -10.07294708911412 1106 62-63 -3.9732001865111535 1106 64-65 -5.422487332783636 1106 66-67 -6.9767730553745295 1106 68-69 -4.162129702135632 1106 70-71 -4.153736510087903 1106 72-73 -4.68985539823249 1106 74-75 -5.119673612982211 1106 76-77 -6.626613990275736 1106 78-79 -9.31779373387026 1106 80-81 -4.964008731136712 1106 82-83 -2.4882278303732157 1106 84-85 -1.9451285521567385 1106 86-87 -1.770245141949431 1106 88-89 -1.6860110581756622 1106 90-91 -4.272960830876432 1106 92-93 -6.509959874051603 1106 94-95 -7.3236378554926205 1106 96-97 -7.113257985979712 1106 98-99 -6.596788244724825 1106 100-101 -7.11132574689621 1107 1 0.0578361248764665 1107 2 -0.07830695780393881 1107 3 -0.04828588184602722 1107 4 0.0328266297525488 1107 5 -0.09970619574254869 1107 6 -0.08856390718749907 1107 7 -0.004395108569433148 1107 8 0.00871024666374609 1107 9 0.02711564905284547 1107 10-11 0.05031986752325679 1107 12-13 -0.07238597399652491 1107 14-15 -0.008530378052931553 1107 16-17 -0.021406125158321743 1107 18-19 0.043013183803132904 1107 20-21 0.06172121606392267 1107 22-23 0.08895992204330838 1107 24-25 0.12342355442204678 1107 26-27 0.11462271830909287 1107 28-29 0.029802375264857517 1107 30-31 0.1467367164710751 1107 32-33 0.14316606474094584 1107 34-35 0.10708051059663859 1107 36-37 -0.049746208406226344 1107 38-39 -0.1254161785271961 1107 40-41 -0.08706163801389977 1107 42-43 -0.16562132142757946 1107 44-45 -0.2696466091977001 1107 46-47 0.005203442005118575 1107 48-49 -0.1334654044450474 1107 50-51 -0.06539459885553356 1107 52-53 -0.07449518460710891 1107 54-55 -0.2608887155271091 1107 56-57 1.0057375610730475 1107 58-59 -9.128541728075323 1107 60-61 -10.792432609894224 1107 62-63 -4.4475844622311165 1107 64-65 -5.960782903695527 1107 66-67 -7.29001726354246 1107 68-69 -4.238538461616656 1107 70-71 -4.2408675125914606 1107 72-73 -2.6155546208265115 1107 74-75 -4.803186875359039 1107 76-77 -6.970939578637694 1107 78-79 -7.780970738865946 1107 80-81 -2.8768090992011786 1107 82-83 -1.8669998276964215 1107 84-85 -1.5563911736857463 1107 86-87 -1.51841031097711 1107 88-89 -1.319262529832205 1107 90-91 -0.8084081064271338 1107 92-93 -3.74944453808196 1107 94-95 -6.425050637821231 1107 96-97 -6.750508948357167 1107 98-99 -6.365879377354955 1107 100-101 -6.9985952208329785 1108 1 -0.046346323706508485 1108 2 -0.017290873920739358 1108 3 -0.033677454162134524 1108 4 -0.04490112311419381 1108 5 -0.06710583334967879 1108 6 -0.09418072770637309 1108 7 -0.08306759631994964 1108 8 -0.03847515323775497 1108 9 -0.07579761765082083 1108 10-11 -0.10159108942097816 1108 12-13 -0.011787286568399225 1108 14-15 -0.025697992456358065 1108 16-17 -0.06470728898661093 1108 18-19 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13.80235969839083 2316 98-99 13.297737022773378 2316 100-101 12.113245416419964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 48.0 5 79.0 6 266.0 7 683.0 8 1382.0 9 2704.0 10 4516.0 11 7054.0 12 10383.0 13 14957.0 14 18539.0 15 25483.0 16 35173.0 17 47210.0 18 53370.0 19 54813.0 20 58178.0 21 57912.0 22 57974.0 23 49714.0 24 50280.0 25 58931.0 26 83491.0 27 123484.0 28 175224.0 29 237768.0 30 295893.0 31 324333.0 32 325554.0 33 297675.0 34 263127.0 35 236224.0 36 192326.0 37 123124.0 38 46875.0 39 3225.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33679484321326 19.79356192722679 14.405868026340077 23.463775203219875 2 15.267777293420279 21.411158098781964 39.65974135309512 23.661323254702637 3 19.108873695392838 25.673746202042075 30.370391895932208 24.84698820663288 4 12.697225784746443 17.33052445649162 38.17810663845806 31.79414312030387 5 13.091595839471024 38.41496086378678 34.85205041356282 13.641392883179378 6 30.96022784976224 37.4624062571806 17.81758418017555 13.759781712881619 7 27.342129450435408 33.1887951664296 21.72305592470674 17.74601945842825 8 25.418596248826375 35.452233741628824 20.309819643361088 18.819350366183716 9 26.042506431267448 16.716052689208983 20.51160144626001 36.729839433263564 10-11 24.39465798119766 26.598230251807305 28.383991079659037 20.623120687335998 12-13 25.730926614797113 24.04658912896358 28.34386212776615 21.87862212847316 14-15 22.579863344984634 25.405879476058406 26.741497632915838 25.27275954604112 16-17 21.848918615369914 28.553853832352356 27.25178243547238 22.345445116805358 18-19 21.74334737423294 27.8342198208411 28.90910433580244 21.51332846912353 20-21 22.827411953724233 26.81605601456446 28.5683985177902 21.788133513921107 22-23 22.3302534230324 26.7567831524253 28.471849408144333 22.44111401639796 24-25 22.365115997254033 26.839382460424893 28.646833265003757 22.148668277317313 26-27 22.317364551001855 26.820355355474558 28.826867374789227 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22.55521682380362 27.39449815276752 27.659356597577982 22.39092842585088 54-55 22.282716482263194 27.13770440048396 27.83805633786348 22.741522779389364 56-57 21.520091690040307 27.3158355260948 28.73284972832051 22.431223055544383 58-59 21.171787528190723 26.690906999030723 29.841526239992376 22.295779232786174 60-61 22.18998736521427 26.996280334741265 28.472491898664533 22.341240401379935 62-63 22.524219953760614 27.05866985514914 27.97812482117964 22.438985369910604 64-65 21.998858444749445 27.25149727898339 28.247913647314014 22.501730628953155 66-67 21.762881280938448 26.7771430072043 28.961549066842473 22.498426645014778 68-69 21.904861319050216 26.433921517896707 29.461934628776405 22.199282534276673 70-71 21.81064976279963 26.371394299458284 29.334870048518365 22.483085889223723 72-73 21.08095204696496 26.462348543296283 29.789504867163537 22.667194542575224 74-75 21.544706065227064 26.927023254242854 28.9602850002392 22.56798568029088 76-77 19.93824018717857 27.2176482653317 29.582889569200617 23.26122197828911 78-79 20.804541042056655 27.212744917184047 28.97286745515963 23.00984658559967 80-81 21.951280921885488 27.348659025651408 27.720963315243658 22.979096737219443 82-83 22.2905901993195 27.30841159293076 27.480192002204923 22.920806205544817 84-85 22.19062796445818 27.199250685331428 27.325344777767775 23.284776572442613 86-87 22.097284475146374 27.18558131153528 27.506165042951956 23.21096917036639 88-89 22.279553856793612 27.096769264944598 27.306102046847702 23.31757483141409 90-91 21.530515831255016 27.14996263833427 27.9269140945983 23.39260743581242 92-93 20.539029404201372 27.465521156498085 28.615718232168824 23.37973120713172 94-95 19.68204929281369 27.40425080556774 29.23354640395751 23.680153497661053 96-97 19.637443919000848 27.343567971782605 28.892240062746254 24.126748046470293 98-99 20.01455928090341 27.36093356868457 28.409438826481235 24.21506832393078 100-101 20.48446135954088 27.30782671337556 28.120766489963422 24.086945437120143 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 504.0 1 379.5 2 263.5 3 277.5 4 315.0 5 405.5 6 537.0 7 745.0 8 1069.0 9 1317.0 10 1383.0 11 1558.0 12 1846.0 13 2127.0 14 2439.0 15 2778.0 16 3099.5 17 3440.5 18 3853.5 19 4397.5 20 5175.0 21 6083.0 22 7342.5 23 9237.0 24 11526.5 25 14556.5 26 18476.5 27 23082.5 28 28353.5 29 34098.5 30 40312.0 31 47249.0 32 54800.0 33 62519.5 34 70514.0 35 78213.5 36 85468.0 37 92099.5 38 97580.0 39 102551.5 40 107708.5 41 112471.5 42 116530.5 43 120765.0 44 125710.5 45 130456.5 46 135570.5 47 141306.0 48 144853.0 49 141752.5 50 134017.5 51 126360.5 52 118626.0 53 111234.5 54 103145.0 55 94329.5 56 85076.0 57 75137.0 58 65300.5 59 55119.5 60 44673.0 61 34675.5 62 26238.5 63 19739.5 64 14376.5 65 10135.0 66 6977.5 67 4765.0 68 3239.0 69 2162.5 70 1402.0 71 887.0 72 591.0 73 366.5 74 232.5 75 140.5 76 78.0 77 49.0 78 29.5 79 16.0 80 6.5 81 5.5 82 5.0 83 2.0 84 1.5 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021479998885556545 2 0.0070102088413113404 3 3.29539731856516E-4 4 0.0 5 0.14257087126410542 6 0.0 7 2.9958157441501456E-5 8 0.0014080333997505683 9 0.0 10-11 0.0 12-13 0.0 14-15 6.441003849922812E-4 16-17 0.0 18-19 7.489539360375364E-5 20-21 0.0 22-23 3.894560467395189E-4 24-25 1.4979078720750728E-5 26-27 2.246861808112609E-4 28-29 0.003535062578097172 30-31 0.012627363361592862 32-33 0.00268125509101438 34-35 0.004763347033198731 36-37 0.11038083109321212 38-39 0.10999137504647258 40-41 0.1228434245888767 42-43 0.12464091403536681 44-45 0.12098601882750362 46-47 0.11400576814363378 48-49 0.11382601919898477 50-51 0.11222325777586445 52-53 0.08987447232450436 54-55 0.07772643948197552 56-57 0.5555590506739237 58-59 1.5744659434168295 60-61 0.5211071696161971 62-63 0.004254058356693206 64-65 0.11806509847695724 66-67 1.891558060856402 68-69 2.8515971742267574 70-71 2.7592811120707705 72-73 2.613100282834964 74-75 1.9980143733247773 76-77 1.4084528139547494 78-79 0.936462043463894 80-81 0.052112214869491774 82-83 1.9472802336975944E-4 84-85 1.64769865928258E-4 86-87 4.643514403432726E-4 88-89 7.489539360375364E-5 90-91 0.25083965225769167 92-93 0.39317085826226505 94-95 1.9933708589213446 96-97 2.409354854075313 98-99 1.4171556586915057 100-101 0.9409707461588399 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3337989.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.42614082089705 #Duplication Level Percentage of deduplicated Percentage of total 1 69.2480777759619 34.22665243731124 2 15.69427403188691 15.5141479676358 3 6.161572121329694 9.136281940408644 4 2.949014518555421 5.830336275079605 5 1.685183849707629 4.164606713237533 6 1.0488535750284311 3.1104470699913924 7 0.6777743447100564 2.3449839144501325 8 0.48742053618701053 1.927305284846106 9 0.35036605891563166 1.558551795014405 >10 1.5760127992843334 13.257120551462585 >50 0.08003787803433962 2.6986023293463184 >100 0.0380152439973872 3.38095734867448 >500 0.001759298672948632 0.6227202009683259 >1k 0.0013953058425999177 1.3691268814395887 >5k 2.4266188566955088E-4 0.8581592901338346 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9739 0.29176249532278264 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7031 0.21063580497119672 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6689 0.20039011512620322 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5161 0.154614050555589 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4706 0.14098308891970585 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4019 0.12040183475739434 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3674 0.11006627044007634 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.9958157441501456E-5 2 0.0 0.0 0.0 0.0 2.9958157441501456E-5 3 0.0 0.0 0.0 0.0 2.9958157441501456E-5 4 0.0 0.0 0.0 0.0 2.9958157441501456E-5 5 0.0 0.0 0.0 0.0 2.9958157441501456E-5 6 2.9958157441501456E-5 0.0 0.0 0.0 2.9958157441501456E-5 7 2.9958157441501456E-5 0.0 0.0 0.0 2.9958157441501456E-5 8 2.9958157441501456E-5 0.0 0.0 0.0 2.9958157441501456E-5 9 2.9958157441501456E-5 0.0 0.0 0.0 2.9958157441501456E-5 10-11 2.9958157441501456E-5 0.0 0.0 1.4979078720750728E-5 2.9958157441501456E-5 12-13 2.9958157441501456E-5 0.0 0.0 2.9958157441501456E-5 1.048535510452551E-4 14-15 2.9958157441501456E-5 0.0 0.0 5.991631488300291E-5 1.64769865928258E-4 16-17 4.493723616225218E-5 0.0 0.0 2.3966525953201165E-4 2.696234169735131E-4 18-19 5.991631488300291E-5 0.0 0.0 3.145606531357653E-4 2.696234169735131E-4 20-21 5.991631488300291E-5 0.0 0.0 4.94309597784774E-4 3.29539731856516E-4 22-23 5.991631488300291E-5 0.0 0.0 7.040166998752842E-4 3.744769680187682E-4 24-25 8.987447232450436E-5 0.0 0.0 0.0010635145891733015 3.8945604673951893E-4 26-27 8.987447232450436E-5 0.0 0.0 0.0015728032656788264 3.8945604673951893E-4 28-29 8.987447232450436E-5 0.0 0.0 0.0029958157441501458 3.8945604673951893E-4 30-31 8.987447232450436E-5 0.0 0.0 0.008313388690016653 3.8945604673951893E-4 32-33 8.987447232450436E-5 0.0 0.0 0.020686107713356754 4.0443512546026964E-4 34-35 8.987447232450436E-5 0.0 0.0 0.039724516767430926 4.1941420418102035E-4 36-37 8.987447232450436E-5 0.0 0.0 0.06640225596908797 4.1941420418102035E-4 38-39 1.7974894464900873E-4 0.0 0.0 0.1091675257168313 4.1941420418102035E-4 40-41 1.7974894464900873E-4 0.0 0.0 0.15855354825914647 4.1941420418102035E-4 42-43 2.0970710209051018E-4 0.0 0.0 0.20826911053331812 4.493723616225218E-4 44-45 2.3966525953201165E-4 0.0 0.0 0.2668522874101742 4.643514403432726E-4 46-47 2.696234169735131E-4 0.0 0.0 0.3365050034616651 5.242677552262755E-4 48-49 2.696234169735131E-4 0.0 0.0 0.4014393097161195 5.692049913885276E-4 50-51 2.696234169735131E-4 0.0 0.0 0.46510039427931005 5.692049913885276E-4 52-53 2.696234169735131E-4 0.0 0.0 0.5264696797982258 5.841840701092784E-4 54-55 2.696234169735131E-4 0.0 1.4979078720750728E-5 0.5940403039075324 5.991631488300291E-4 56-57 2.696234169735131E-4 0.0 2.9958157441501456E-5 0.6514551126441699 5.991631488300291E-4 58-59 2.696234169735131E-4 0.0 2.9958157441501456E-5 0.6899962821926615 6.141422275507799E-4 60-61 2.696234169735131E-4 2.9958157441501456E-5 2.9958157441501456E-5 0.7245230586439919 6.291213062715306E-4 62-63 3.29539731856516E-4 2.9958157441501456E-5 2.9958157441501456E-5 0.7559042285639648 6.291213062715306E-4 64-65 3.29539731856516E-4 2.9958157441501456E-5 2.9958157441501456E-5 0.7858474069267454 6.291213062715306E-4 66-67 3.29539731856516E-4 2.9958157441501456E-5 2.9958157441501456E-5 0.8104730123436596 6.291213062715306E-4 68-69 3.29539731856516E-4 2.9958157441501456E-5 2.9958157441501456E-5 0.8447900816928995 6.291213062715306E-4 70-71 3.445188105772667E-4 2.9958157441501456E-5 2.9958157441501456E-5 0.8998232169129377 6.291213062715306E-4 72-73 3.5949788929801746E-4 2.9958157441501456E-5 2.9958157441501456E-5 0.9816689030431196 6.59079463713032E-4 74-75 3.5949788929801746E-4 2.9958157441501456E-5 2.9958157441501456E-5 1.0878106548583593 6.890376211545335E-4 76-77 3.5949788929801746E-4 2.9958157441501456E-5 2.9958157441501456E-5 1.2536290562970698 6.890376211545335E-4 78-79 3.5949788929801746E-4 2.9958157441501456E-5 2.9958157441501456E-5 1.5092020974305187 7.489539360375363E-4 80-81 4.1941420418102035E-4 2.9958157441501456E-5 2.9958157441501456E-5 1.8245117045023216 7.938911721997885E-4 82-83 4.1941420418102035E-4 2.9958157441501456E-5 2.9958157441501456E-5 2.13952172999971 8.687865658035422E-4 84-85 4.3439328290177106E-4 2.9958157441501456E-5 2.9958157441501456E-5 2.386526738104889 8.83765644524293E-4 86-87 4.493723616225218E-4 2.9958157441501456E-5 2.9958157441501456E-5 2.579307481240951 9.137238019657943E-4 88-89 4.493723616225218E-4 2.9958157441501456E-5 2.9958157441501456E-5 2.756210400933017 9.287028806865451E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 12355 0.0 23.582697 2 GTACATG 12565 0.0 23.565292 1 ACATGGG 12420 0.0 22.24485 3 GTATCAA 22160 0.0 19.84092 1 GAGTACT 8320 0.0 19.402552 12-13 CATGGGA 7675 0.0 18.88797 4 AGTACTT 9125 0.0 18.4119 12-13 TCAACGC 24645 0.0 17.608204 4 AACGCAG 24765 0.0 17.55304 6 ATCAACG 24780 0.0 17.531271 3 CAACGCA 24880 0.0 17.44189 5 GTACTTT 9225 0.0 17.218657 14-15 TATCAAC 25690 0.0 16.911541 2 GGTATCA 17540 0.0 16.264013 1 CGCAGAG 26460 0.0 16.09164 8 ACGCAGA 26730 0.0 16.086851 7 GTGTAGC 2125 0.0 15.94991 1 CATGGGG 5525 0.0 15.589486 4 ATGGGAT 3325 0.0 15.429358 5 AGAGTAC 19490 0.0 15.275317 10-11 >>END_MODULE