FastQCFastQC Report
Fri 27 May 2016
SRR939608_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939608_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290203
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37980.11543360698412833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG103700.029.746821
TACATGG119650.026.1782132
ACATGGG120300.024.8590133
GTATCAA202150.022.7644481
GAGTACT80150.022.3159412-13
CATGGGA70200.021.3305364
GTACTTT89550.019.89788414-15
GTCTAGG9200.019.7881031
TATCAAC244350.019.2955592
AGTACTT89900.018.95077712-13
CATGGGG56400.018.6769264
ATCAACG256550.018.6360663
CAACGCA258700.018.5355955
TCAACGC257300.018.4871224
AACGCAG265050.018.1271386
ACGCAGA271050.017.4295967
GGTATCA164550.016.902631
CGCAGAG280550.016.7550268
GTATATG17950.016.3400361
ACTTTTT116100.016.1849816-17
Produced by FastQC (version 0.11.5)