FastQCFastQC Report
Fri 27 May 2016
SRR939608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939608_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290203
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95040.2888575568133638No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT68640.20861934658742942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66890.20330052583381633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50950.15485366708376352No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA44670.1357666988936549No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA37530.11406591021891355No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36200.11002360644616761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG118850.023.6280942
GTACATG122850.023.4788071
ACATGGG120100.022.1615833
GAGTACT83100.020.31931312-13
GTATCAA221250.020.269841
AGTACTT91200.019.34210812-13
CATGGGA71600.018.9161854
ATCAACG245450.018.1306483
CATGGGG55000.018.1044124
TCAACGC246550.018.0497574
CAACGCA248950.017.8947055
AACGCAG249750.017.8645826
GTACTTT95450.017.59142914-15
TATCAAC254850.017.4637682
ACGCAGA268850.016.384917
CGCAGAG267550.016.305878
AGAGTAC190550.016.17547410-11
ATGGGAG36100.016.0791325
TACGCGG1401.0816677E-515.61645564-65
TACTTTT111950.015.56744714-15
Produced by FastQC (version 0.11.5)