##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939608_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3290203 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.486580615238633 31.0 31.0 34.0 30.0 34.0 2 31.69725910528925 31.0 31.0 34.0 30.0 34.0 3 31.75460298346333 31.0 31.0 34.0 30.0 34.0 4 35.54363788495725 37.0 35.0 37.0 33.0 37.0 5 35.300166585466 37.0 35.0 37.0 33.0 37.0 6 35.30464928759715 37.0 35.0 37.0 32.0 37.0 7 35.21390017576423 37.0 35.0 37.0 32.0 37.0 8 35.1735196886028 37.0 35.0 37.0 32.0 37.0 9 36.776330822140764 39.0 35.0 39.0 32.0 39.0 10-11 36.65915552930929 39.0 35.0 39.0 32.0 39.0 12-13 36.594220934088256 39.0 35.0 39.0 32.0 39.0 14-15 37.671508110593784 39.0 36.5 41.0 32.0 41.0 16-17 37.624973595854115 39.0 36.0 41.0 32.0 41.0 18-19 37.5971432461766 39.0 36.0 41.0 32.0 41.0 20-21 37.5349565057232 39.0 36.0 41.0 32.0 41.0 22-23 37.39994249594933 39.0 36.0 41.0 32.0 41.0 24-25 37.26425937852467 39.0 36.0 41.0 31.0 41.0 26-27 36.97404521848652 39.0 36.0 40.0 31.0 41.0 28-29 36.78517784464971 39.0 36.0 40.0 30.0 41.0 30-31 36.560652792548055 38.0 35.0 40.0 30.0 41.0 32-33 36.41209448170827 38.0 35.0 40.0 30.0 41.0 34-35 36.392354970194845 38.0 35.0 40.0 30.0 41.0 36-37 36.322432384871085 38.0 35.0 40.0 30.0 41.0 38-39 36.366277399905115 38.0 35.0 40.0 30.0 41.0 40-41 36.2664139568288 38.0 35.0 40.0 30.0 41.0 42-43 36.102542609073055 38.0 35.0 40.0 29.5 41.0 44-45 35.90081994940738 38.0 35.0 40.0 29.0 41.0 46-47 35.64282340633694 38.0 34.5 40.0 28.0 41.0 48-49 35.36783216719455 38.0 34.0 40.0 27.0 41.0 50-51 35.142472212200886 38.0 34.0 40.0 26.5 41.0 52-53 34.91826005872586 38.0 34.0 40.0 26.0 41.0 54-55 34.63833903257641 38.0 33.0 40.0 26.0 41.0 56-57 33.85046135451217 37.5 32.5 40.0 22.5 41.0 58-59 30.711641348573323 35.5 27.0 39.0 8.5 40.5 60-61 30.439258763061126 34.5 26.0 39.0 10.5 40.0 62-63 32.27516068157497 35.5 30.5 39.0 20.0 40.0 64-65 32.324866277247935 36.0 31.0 39.0 18.5 40.0 66-67 27.38275860182487 32.0 18.5 37.0 4.5 39.5 68-69 19.788685378987253 20.0 8.0 32.0 2.0 37.0 70-71 17.831830133277492 15.5 7.5 29.0 2.0 35.0 72-73 19.56203219071893 20.0 7.5 31.0 2.0 35.5 74-75 21.022151368775724 23.5 9.5 32.5 2.0 36.0 76-77 22.891917459196286 27.5 12.0 33.0 2.0 35.5 78-79 25.106120047911936 30.5 13.0 34.5 2.0 36.5 80-81 26.731291655864396 32.0 22.5 35.0 2.0 37.0 82-83 27.874198947602927 33.0 26.5 35.0 2.0 36.0 84-85 27.922735162541642 34.0 27.5 35.0 2.0 36.0 86-87 27.64787613408656 33.5 27.0 35.0 2.0 36.0 88-89 27.202688101615614 33.0 26.0 35.0 2.0 35.5 90-91 25.35062851745014 32.5 16.0 34.5 2.0 35.0 92-93 23.723317375857963 31.5 2.0 34.0 2.0 35.0 94-95 19.901850280970507 27.5 2.0 34.0 2.0 35.0 96-97 17.184095783755595 18.5 2.0 33.0 2.0 35.0 98-99 17.450653652677357 21.5 2.0 33.0 2.0 35.0 100-101 16.658665134035804 10.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.26348212863087994 1101 2 0.17878965714609407 1101 3 0.1586549490530622 1101 4 0.19347893185904752 1101 5 0.36260528153992766 1101 6 0.24314696786860424 1101 7 0.29262833060941773 1101 8 0.27746166700180197 1101 9 0.2936518482807102 1101 10-11 0.300026651129933 1101 12-13 0.24788101158024034 1101 14-15 0.38560486037304287 1101 16-17 0.37071680120198636 1101 18-19 0.29384371187887126 1101 20-21 0.3576221194532607 1101 22-23 0.30163509502880714 1101 24-25 0.25585615887895585 1101 26-27 0.2804376979235741 1101 28-29 0.21951720410837083 1101 30-31 0.06890929677919644 1101 32-33 0.22248603686546886 1101 34-35 0.22435577473359558 1101 36-37 0.05280236222127854 1101 38-39 0.04020811827304982 1101 40-41 0.1261156925397131 1101 42-43 0.15566702901108442 1101 44-45 0.10859265476604918 1101 46-47 0.06601728472310242 1101 48-49 -0.1626549741986949 1101 50-51 -0.15666702710866787 1101 52-53 -0.0863296535023963 1101 54-55 -0.055938851555076496 1101 56-57 -3.4682621596999184 1101 58-59 -10.523512214600274 1101 60-61 -11.970880326784435 1101 62-63 -4.295791794616786 1101 64-65 -1.3538575421887415 1101 66-67 -2.311579166527192 1101 68-69 -4.800761285594248 1101 70-71 -4.672690482217462 1101 72-73 -3.569835111963698 1101 74-75 -6.377162212342393 1101 76-77 -5.019769438732169 1101 78-79 1.0858357358437019 1101 80-81 1.650081188872658 1101 82-83 0.19647112869777317 1101 84-85 -0.07634389341856362 1101 86-87 -0.16637587779927188 1101 88-89 -0.3212260466118124 1101 90-91 1.1425755964378972 1101 92-93 0.6888893785830525 1101 94-95 -8.554607844808453 1101 96-97 -9.495287565606887 1101 98-99 -8.707814948223765 1101 100-101 -9.572682352792667 1102 1 0.24678663919809196 1102 2 0.3139069467405342 1102 3 0.2512488742795078 1102 4 0.153794666766359 1102 5 0.25451069236724067 1102 6 0.24482078404576413 1102 7 0.3513194614036692 1102 8 0.34421584983788023 1102 9 0.26764293152939445 1102 10-11 0.3173242245383534 1102 12-13 0.34936328198096334 1102 14-15 0.49888426496008975 1102 16-17 0.4487794063204902 1102 18-19 0.43562899628495444 1102 20-21 0.34737734940288334 1102 22-23 0.35843324047382197 1102 24-25 0.38392380135343274 1102 26-27 0.12725953030410864 1102 28-29 0.314637634790941 1102 30-31 0.28503926409824487 1102 32-33 0.22372530218414965 1102 34-35 0.23382486771410527 1102 36-37 -0.014930633167331564 1102 38-39 0.2256555377454248 1102 40-41 0.1157591916613967 1102 42-43 0.21562999750096878 1102 44-45 0.05984279016866623 1102 46-47 -0.022103619900185834 1102 48-49 0.12953275581215706 1102 50-51 0.10946797756635362 1102 52-53 0.08026896467132616 1102 54-55 -0.15047083321051957 1102 56-57 -3.4708506536718815 1102 58-59 -10.111529089616528 1102 60-61 -8.208054827107762 1102 62-63 -2.419186310994 1102 64-65 -0.7256968538157409 1102 66-67 -1.9404688196465294 1102 68-69 -5.1251730701287315 1102 70-71 -4.872450178875209 1102 72-73 -3.505348244312838 1102 74-75 -4.1548471546375225 1102 76-77 -4.118183088224608 1102 78-79 1.6229183770140274 1102 80-81 1.9748712169783254 1102 82-83 0.5051932537499475 1102 84-85 0.2721575626171848 1102 86-87 0.15700962436199362 1102 88-89 0.1392557064183677 1102 90-91 1.5764584686953498 1102 92-93 0.936012593825108 1102 94-95 -9.124865956908433 1102 96-97 -9.89233789699926 1102 98-99 -8.942287961251958 1102 100-101 -9.691599934831501 1103 1 0.23781635343786078 1103 2 0.17183813070522902 1103 3 0.19538769855012816 1103 4 0.17453935468762438 1103 5 0.19991543407775936 1103 6 0.25070121682252733 1103 7 0.22584644463287162 1103 8 0.3215897365640359 1103 9 0.3551289657869603 1103 10-11 0.37467630264816165 1103 12-13 0.32061160917022136 1103 14-15 0.4499340981866382 1103 16-17 0.46868054341998544 1103 18-19 0.3515793440086057 1103 20-21 0.3724905288967477 1103 22-23 0.3081811130043164 1103 24-25 0.37953826670269564 1103 26-27 0.5390826397884254 1103 28-29 0.4597635223867087 1103 30-31 0.3743837685577063 1103 32-33 0.36283632870505045 1103 34-35 0.33808345181920174 1103 36-37 0.386901481422818 1103 38-39 0.32210782124471393 1103 40-41 0.3884386970663769 1103 42-43 0.16705053806337844 1103 44-45 0.31141729740965474 1103 46-47 0.22582338320989237 1103 48-49 0.1733389709579356 1103 50-51 0.1594772207860018 1103 52-53 0.3160610107401496 1103 54-55 0.2676986619139754 1103 56-57 0.7049657811233061 1103 58-59 -8.377058085967597 1103 60-61 -7.977724109030689 1103 62-63 -2.1320219831840106 1103 64-65 -0.22536055881946737 1103 66-67 -1.692719831079824 1103 68-69 -3.6800875395634396 1103 70-71 -3.832889851149048 1103 72-73 -3.0101199800068272 1103 74-75 -3.926600177113265 1103 76-77 -0.6135537315469861 1103 78-79 3.414637528614488 1103 80-81 2.8965435947070866 1103 82-83 1.3043244318759655 1103 84-85 0.947946964970253 1103 86-87 0.9658701983647084 1103 88-89 0.7357282540560135 1103 90-91 2.069536981100466 1103 92-93 1.4821783375153146 1103 94-95 -7.209806741997422 1103 96-97 -8.788232578278613 1103 98-99 -8.21009783519597 1103 100-101 -9.545822047564856 1104 1 0.24048167904801332 1104 2 0.17614813913041516 1104 3 0.19542103126200416 1104 4 0.08382619526388169 1104 5 0.3508164140514225 1104 6 0.18167482013952707 1104 7 0.19532425340011628 1104 8 0.26205864307594595 1104 9 0.2364848501122765 1104 10-11 0.2704985032189242 1104 12-13 0.32880592774265693 1104 14-15 0.4692482659370185 1104 16-17 0.39029814683674857 1104 18-19 0.3989874996757621 1104 20-21 0.27261946918849134 1104 22-23 0.3367095560979081 1104 24-25 0.3120408972024791 1104 26-27 0.4328279218578288 1104 28-29 0.43288625916214585 1104 30-31 0.44934911827555624 1104 32-33 0.3995683315224383 1104 34-35 0.3440856401561305 1104 36-37 0.2163175754407618 1104 38-39 0.05487991403297343 1104 40-41 0.13455368071760887 1104 42-43 0.150172321668542 1104 44-45 0.21737683924546758 1104 46-47 0.14043462007867902 1104 48-49 0.017326867551631153 1104 50-51 0.0681891326139592 1104 52-53 0.01809340222663991 1104 54-55 0.07962262846472612 1104 56-57 0.5341335746858249 1104 58-59 -8.55399025938712 1104 60-61 -7.830955093989726 1104 62-63 -2.4008043811877293 1104 64-65 -0.6556168631611712 1104 66-67 -1.708330799107781 1104 68-69 -3.463309983115945 1104 70-71 -3.8570404501168944 1104 72-73 -3.0218355807894106 1104 74-75 -3.7865870086743563 1104 76-77 -0.20294996637101903 1104 78-79 3.5106644307763624 1104 80-81 2.904103381122688 1104 82-83 1.2464797879786857 1104 84-85 0.8552600328218567 1104 86-87 0.9227884753524442 1104 88-89 0.6647537903837843 1104 90-91 2.13591407285929 1104 92-93 1.7530362819717205 1104 94-95 -6.385379255536515 1104 96-97 -7.950921433716417 1104 98-99 -5.793820850529583 1104 100-101 -5.0715304060288755 1105 1 0.345900675983831 1105 2 0.31902594577512744 1105 3 0.25645730393776134 1105 4 0.2251300558532776 1105 5 0.22366033289805642 1105 6 0.3385963551009823 1105 7 0.1930015297827552 1105 8 0.2891337368256828 1105 9 0.2941350276459431 1105 10-11 0.3589203145788744 1105 12-13 0.28897163667530634 1105 14-15 0.413135832153678 1105 16-17 0.4463845068251473 1105 18-19 0.510410587878809 1105 20-21 0.4301560772440425 1105 22-23 0.5185989665982831 1105 24-25 0.4981164331084571 1105 26-27 0.4136707196533109 1105 28-29 0.49879304351478027 1105 30-31 0.44309108207569636 1105 32-33 0.3899178139311701 1105 34-35 0.3838904405817658 1105 36-37 0.4761184546279722 1105 38-39 0.4850869546084411 1105 40-41 0.3958512608172384 1105 42-43 0.4959831251685287 1105 44-45 0.4912110335021609 1105 46-47 0.5279101275922855 1105 48-49 0.4800462626721256 1105 50-51 0.5765942502368802 1105 52-53 0.7046572218057392 1105 54-55 0.6844624698742265 1105 56-57 1.127186352358521 1105 58-59 -8.352598870573146 1105 60-61 -7.616030592066327 1105 62-63 -1.8767641795449563 1105 64-65 0.1683687502868807 1105 66-67 -1.3550696019178936 1105 68-69 -3.2919092345824446 1105 70-71 -3.8033550351306857 1105 72-73 -2.7041112576809923 1105 74-75 -3.461103270627728 1105 76-77 0.11160425512281691 1105 78-79 4.159073583532596 1105 80-81 3.5479793544664027 1105 82-83 2.0276415321487953 1105 84-85 1.6128928007077263 1105 86-87 1.5843114046314462 1105 88-89 1.4592615753030458 1105 90-91 2.9909565454848526 1105 92-93 2.3489090158192845 1105 94-95 -6.155077438140129 1105 96-97 -7.599188299211024 1105 98-99 -5.59222518309128 1105 100-101 -5.119458149482259 1106 1 0.17728441350166335 1106 2 0.1603954179577194 1106 3 0.211093537889262 1106 4 0.116428201359696 1106 5 0.1423479220564019 1106 6 0.16969478591411047 1106 7 0.2754551674432335 1106 8 0.1721579878955808 1106 9 0.21453445703253493 1106 10-11 0.17946239731782754 1106 12-13 0.16486322012752197 1106 14-15 0.16862798621929898 1106 16-17 0.3138595424884798 1106 18-19 0.19043911854927842 1106 20-21 0.21460686183747413 1106 22-23 0.21389140597060674 1106 24-25 0.18128333753071502 1106 26-27 0.21352806630095955 1106 28-29 0.2724021733293327 1106 30-31 0.4069997839301891 1106 32-33 0.3525426356144763 1106 34-35 0.3149888713948883 1106 36-37 0.4247632477806107 1106 38-39 0.4317346008281291 1106 40-41 0.2927128870654627 1106 42-43 0.32380052887751276 1106 44-45 0.4029086083951725 1106 46-47 0.21678316610869075 1106 48-49 0.2265512555394622 1106 50-51 0.21553861542945896 1106 52-53 0.31992113050759485 1106 54-55 0.33139542279861445 1106 56-57 0.9110665235425444 1106 58-59 -8.595109569574575 1106 60-61 -7.608469964391578 1106 62-63 -2.0114289441669264 1106 64-65 -0.12492654215248677 1106 66-67 0.6978547918053621 1106 68-69 -2.3188707181773047 1106 70-71 -2.443440627142099 1106 72-73 -0.8122424987651549 1106 74-75 -0.8857823005849319 1106 76-77 2.385498691642077 1106 78-79 4.679758319623897 1106 80-81 3.610746829502066 1106 82-83 2.0022492354298187 1106 84-85 1.5191695482112912 1106 86-87 1.4967222462862217 1106 88-89 1.3499891074000487 1106 90-91 2.708360254472016 1106 92-93 2.4500502426381168 1106 94-95 -6.2175596064710845 1106 96-97 -7.548403705773078 1106 98-99 -5.413908492649053 1106 100-101 -4.972667849910218 1107 1 0.03619736818641073 1107 2 0.020352415090457043 1107 3 0.018946570459412726 1107 4 -0.037683658824605004 1107 5 -0.02723012498391597 1107 6 0.02527735064859371 1107 7 0.0778109555310138 1107 8 0.031040440640893507 1107 9 0.05356777269474833 1107 10-11 0.01642052651057213 1107 12-13 0.08714995701624417 1107 14-15 0.12559469813862023 1107 16-17 0.12674584123632116 1107 18-19 0.1971991514362159 1107 20-21 0.07889497212470076 1107 22-23 0.05532999424999474 1107 24-25 0.06183510625786681 1107 26-27 0.11998458055391836 1107 28-29 0.07021455151931377 1107 30-31 0.0698131741193464 1107 32-33 0.10119623052356985 1107 34-35 0.10081605600504417 1107 36-37 0.08580565967280762 1107 38-39 0.26105609086409487 1107 40-41 -0.04549752520962613 1107 42-43 -0.06587383452632167 1107 44-45 -0.08513787623603264 1107 46-47 0.02783630640909962 1107 48-49 -0.058066194571409824 1107 50-51 0.051470427370396976 1107 52-53 -0.019589548618895947 1107 54-55 0.017689668356261734 1107 56-57 0.5125953521342197 1107 58-59 -8.572581129152525 1107 60-61 -7.680984066468426 1107 62-63 -2.2213345301185115 1107 64-65 -0.46961967114445713 1107 66-67 0.3795558995692758 1107 68-69 -2.4318337585067944 1107 70-71 -2.467884037729437 1107 72-73 -1.2123726187408046 1107 74-75 -1.0455015691711367 1107 76-77 2.1798869026424867 1107 78-79 4.247124367639302 1107 80-81 3.179501040250191 1107 82-83 1.5190531270230068 1107 84-85 0.9775192716049403 1107 86-87 0.7946425169747116 1107 88-89 0.6835911714727771 1107 90-91 1.9745210297080114 1107 92-93 1.7552627077099494 1107 94-95 -6.686925843501168 1107 96-97 -8.24922202575708 1107 98-99 -6.208084163539617 1107 100-101 -5.400664979807512 1108 1 -0.0359203302928357 1108 2 -0.11153862598655806 1108 3 -0.061693703624104756 1108 4 -0.08023804224482944 1108 5 -0.027208339136564064 1108 6 0.005621089823002023 1108 7 0.0013731155411207396 1108 8 -0.03576613957878294 1108 9 0.043167863540936935 1108 10-11 -0.06467314058300389 1108 12-13 -0.07539353188426645 1108 14-15 -0.16494363653364985 1108 16-17 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1.5825766673804047 2311 82-83 0.5236271429703514 2311 84-85 0.3577928167917648 2311 86-87 0.12897093901993628 2311 88-89 0.1789543584765667 2311 90-91 1.7631153856893427 2311 92-93 2.130965109002343 2311 94-95 -1.3522681056589008 2311 96-97 -6.355459146637363 2311 98-99 -5.9069083528386415 2311 100-101 -6.389585065400901 2312 1 -0.29241251395267653 2312 2 -0.24760974296486182 2312 3 -0.2551745203058715 2312 4 -0.2132416049198369 2312 5 -0.26472031147380193 2312 6 -0.22695255052631325 2312 7 -0.27101900238704957 2312 8 -0.29507789817380115 2312 9 -0.25880632649217006 2312 10-11 -0.28698866297916226 2312 12-13 -0.3978367617503906 2312 14-15 -0.4624411704531113 2312 16-17 -0.4262920325067583 2312 18-19 -0.38273244805093753 2312 20-21 -0.5245912512970037 2312 22-23 -0.39435205265294826 2312 24-25 -0.5695377324158954 2312 26-27 -0.5354347152690409 2312 28-29 -0.43072400286382617 2312 30-31 -0.32489195286275674 2312 32-33 -0.38388221735653616 2312 34-35 -0.38166098200206733 2312 36-37 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2313 1 -0.4291165181499146 2313 2 -0.3875763697530701 2313 3 -0.33808995326947766 2313 4 -0.27654160033205244 2313 5 -0.3512174535175774 2313 6 -0.37152755084980527 2313 7 -0.3410906895046608 2313 8 -0.39072679363459883 2313 9 -0.4682573448044991 2313 10-11 -0.5037313222253772 2313 12-13 -0.466826495694022 2313 14-15 -0.5230723088243039 2313 16-17 -0.5144009289321687 2313 18-19 -0.6317568080251945 2313 20-21 -0.484988206653135 2313 22-23 -0.5314704281527511 2313 24-25 -0.46382981918860366 2313 26-27 -0.5739422786481398 2313 28-29 -0.6138508269504115 2313 30-31 -0.645333015676627 2313 32-33 -0.6669082571667744 2313 34-35 -0.5627978533428717 2313 36-37 -0.724484846954816 2313 38-39 -0.6431157908890199 2313 40-41 -0.6169202326227108 2313 42-43 -0.6683366517172971 2313 44-45 -0.7333866237681335 2313 46-47 -0.9451972812633471 2313 48-49 -0.8663166564203877 2313 50-51 -0.8416278222396869 2313 52-53 -0.7623297709286803 2313 54-55 -0.8707801567402882 2313 56-57 0.00545706298241555 2313 58-59 3.102806694607686 2313 60-61 3.0001770620665233 2313 62-63 0.18976065805741626 2313 64-65 -0.597365427632603 2313 66-67 -2.3685951257400752 2313 68-69 -5.126166208445813 2313 70-71 -3.749235088853661 2313 72-73 -3.2143998022922347 2313 74-75 -3.868560578945978 2313 76-77 0.013555847260963816 2313 78-79 2.4004660456243627 2313 80-81 1.8131935740536527 2313 82-83 0.3015577994221381 2313 84-85 -0.056783238629360966 2313 86-87 -0.10944411101482743 2313 88-89 0.0547577941391566 2313 90-91 1.69036757878661 2313 92-93 3.0903497374010556 2313 94-95 5.423063872137867 2313 96-97 0.960568579601631 2313 98-99 2.51012407651773 2313 100-101 3.9275279700219947 2314 1 -0.33978095852223333 2314 2 -0.29580882876800274 2314 3 -0.33882241599985363 2314 4 -0.17094257407575242 2314 5 -0.2604147391624352 2314 6 -0.2898446922759774 2314 7 -0.34192153720562146 2314 8 -0.494313386527395 2314 9 -0.4135582735170047 2314 10-11 -0.4293294716367768 2314 12-13 -0.48018608235825155 2314 14-15 -0.5477581679911268 2314 16-17 -0.5311574722014925 2314 18-19 -0.49446017288036614 2314 20-21 -0.45082112757212656 2314 22-23 -0.42544256529129854 2314 24-25 -0.5447322114890127 2314 26-27 -0.6594701392803302 2314 28-29 -0.5755434422815213 2314 30-31 -0.5645124003098445 2314 32-33 -0.5771314141948309 2314 34-35 -0.49283945145958086 2314 36-37 -0.5013898668175756 2314 38-39 -0.40915896881273994 2314 40-41 -0.5799904233141859 2314 42-43 -0.7062374924480821 2314 44-45 -0.6406288425127045 2314 46-47 -0.5581975101347325 2314 48-49 -0.571115413084037 2314 50-51 -0.5192554960593228 2314 52-53 -0.4960429341540973 2314 54-55 -0.5431459437383168 2314 56-57 0.22141779519249383 2314 58-59 3.51445955468256 2314 60-61 3.5546646130981507 2314 62-63 0.8056421026816878 2314 64-65 -0.1827226750529647 2314 66-67 -1.8021454038782494 2314 68-69 -4.6040271378716895 2314 70-71 -3.4923077168767342 2314 72-73 -2.81018645106532 2314 74-75 -4.046426412758603 2314 76-77 0.6186232305625694 2314 78-79 3.510798436720968 2314 80-81 2.654532798595799 2314 82-83 1.1667645564781353 2314 84-85 0.7317492648222874 2314 86-87 0.882592911160426 2314 88-89 0.9147578387244906 2314 90-91 2.538178327418951 2314 92-93 4.151708074189752 2314 94-95 6.696968336170137 2314 96-97 4.00781658700874 2314 98-99 6.368208177833441 2314 100-101 6.681305016600291 2315 1 -0.40110557833091676 2315 2 -0.33957788390656773 2315 3 -0.41904107442694993 2315 4 -0.3150322340568792 2315 5 -0.4739874665594499 2315 6 -0.34546359406197524 2315 7 -0.5344902040636939 2315 8 -0.49158357415073084 2315 9 -0.5659385423055454 2315 10-11 -0.4666882528370664 2315 12-13 -0.6099378471660017 2315 14-15 -0.7349629130339466 2315 16-17 -0.6306988956231834 2315 18-19 -0.600041021355274 2315 20-21 -0.6609229144826969 2315 22-23 -0.6980150146940645 2315 24-25 -0.622683507808091 2315 26-27 -0.6119769865976892 2315 28-29 -0.6268928537084548 2315 30-31 -0.5729219541974402 2315 32-33 -0.5723126573304214 2315 34-35 -0.47473518994446806 2315 36-37 -0.26370991111436837 2315 38-39 -0.6344813965684111 2315 40-41 -0.6589183453299654 2315 42-43 -0.6307691177118357 2315 44-45 -0.5802877982569328 2315 46-47 -0.4556580782456052 2315 48-49 -0.41170211766729636 2315 50-51 -0.3421504871498371 2315 52-53 -0.29089742011539244 2315 54-55 -0.3201119555234513 2315 56-57 0.446128521668939 2315 58-59 3.634261973688254 2315 60-61 3.640377987355887 2315 62-63 0.9114332697562304 2315 64-65 0.237336432904506 2315 66-67 -1.7578811999671942 2315 68-69 -4.755774058287859 2315 70-71 -3.661937300933534 2315 72-73 -2.920090565835931 2315 74-75 -0.24514912033144043 2315 76-77 2.245649512412278 2315 78-79 3.8686685222611636 2315 80-81 2.7883150242815553 2315 82-83 1.0463758430446326 2315 84-85 0.7491831500425654 2315 86-87 0.7768892439149866 2315 88-89 0.9673247282422892 2315 90-91 2.4729841188138906 2315 92-93 4.075217500929153 2315 94-95 6.870320308789982 2315 96-97 4.551904310144696 2315 98-99 6.74781183095131 2315 100-101 6.973196585171447 2316 1 -0.4376145559762534 2316 2 -0.4482887094367811 2316 3 -0.5989070955059645 2316 4 -0.3540378540341038 2316 5 -0.5346244168968894 2316 6 -0.5179535689911816 2316 7 -0.42287669261546057 2316 8 -0.4484226562633822 2316 9 -0.6714635647028828 2316 10-11 -0.6656876476373057 2316 12-13 -0.5505825008893694 2316 14-15 -0.6296232852519026 2316 16-17 -0.7290985566247272 2316 18-19 -0.6917422388366035 2316 20-21 -0.6690546485671334 2316 22-23 -0.9285314940951608 2316 24-25 -0.6077317567953884 2316 26-27 -0.7022176609702129 2316 28-29 -0.602891827621157 2316 30-31 -0.6536742202948389 2316 32-33 -0.5585362834388121 2316 34-35 -0.5662984824909856 2316 36-37 -0.4302272492991932 2316 38-39 -0.6430815651708244 2316 40-41 -0.7088913556468128 2316 42-43 -0.606452657917103 2316 44-45 -0.48778581983815883 2316 46-47 -0.5922549057247011 2316 48-49 -0.636335449176066 2316 50-51 -0.602298909532891 2316 52-53 -0.5550786005982005 2316 54-55 -0.4233527508122279 2316 56-57 0.353304344726709 2316 58-59 3.383892382796496 2316 60-61 3.462961680113562 2316 62-63 0.6653353702663978 2316 64-65 0.06120126584988128 2316 66-67 -1.99154173392224 2316 68-69 -3.1877182695072186 2316 70-71 -3.192615161785831 2316 72-73 -0.7095146766607776 2316 74-75 5.5262142846579145 2316 76-77 5.604236793073749 2316 78-79 4.7194214888069475 2316 80-81 3.044272159933204 2316 82-83 1.2382528143120233 2316 84-85 0.8644458738497356 2316 86-87 0.5713221018402415 2316 88-89 0.9086124647054632 2316 90-91 2.4666364793249294 2316 92-93 4.0421299739510665 2316 94-95 6.756147918702393 2316 96-97 3.8052886896201095 2316 98-99 6.152720860797832 2316 100-101 6.005685290634602 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 27.0 4 48.0 5 122.0 6 247.0 7 709.0 8 1565.0 9 2752.0 10 4514.0 11 6889.0 12 10048.0 13 14038.0 14 17508.0 15 23021.0 16 31006.0 17 40863.0 18 44826.0 19 45324.0 20 48868.0 21 50410.0 22 50978.0 23 51881.0 24 54305.0 25 58616.0 26 72149.0 27 102162.0 28 143554.0 29 191180.0 30 233437.0 31 260924.0 32 271959.0 33 270969.0 34 276444.0 35 294534.0 36 296269.0 37 224959.0 38 87534.0 39 5558.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.178172430188965 19.87624752683543 14.432002111082332 23.513577931893273 2 15.280612733423451 21.446399352176115 39.63425551168572 23.63873240271471 3 19.11547438098616 25.673205188874938 30.355270952190345 24.85604947794856 4 12.682560924052408 17.382088582376223 38.21253582225778 31.722814671313593 5 13.093146431183747 38.376579039809975 34.877790264279604 13.65248426472668 6 30.926723974174237 37.57716469166188 17.73908783135873 13.757023502805147 7 27.330988391901656 33.22670364108233 21.75415924184617 17.688148725169846 8 25.39856215515035 35.46833902599293 20.317232802119747 18.815866016736965 9 26.05243506251742 16.688210423490588 20.50809630895115 36.75125820504084 10-11 24.36855719844642 26.626244642047926 28.389433721870656 20.615764437635004 12-13 25.723139271345875 24.065779527889315 28.33998388549278 21.87109731527204 14-15 22.561436051023808 25.400421465085827 26.76342806394586 25.274714419944505 16-17 21.812517618596424 28.612813345075267 27.240451005675183 22.334218030653126 18-19 21.72525253139261 27.871776207112976 28.948022679575768 21.454948581918647 20-21 22.77519046697119 26.84066910157215 28.578039105793778 21.806101325662887 22-23 22.275662018422572 26.767466931371715 28.485020529128448 22.471850521077272 24-25 22.356481281673627 26.840892250253635 28.687498442342985 22.115128025729756 26-27 22.32113386489202 26.82273224894064 28.83929781733501 22.016836068832333 28-29 22.004931664562925 27.245319158571586 28.76546995942759 21.984279217437898 30-31 22.20554047714249 27.031189898795553 28.590555660164735 22.172713963897216 32-33 22.127186833444657 27.001787992562313 28.54280505865877 22.32822011533426 34-35 22.491949049576775 26.943462427656943 28.428900963013877 22.135687559752405 36-37 22.60992651918382 26.68353419164638 28.653335399944847 22.05320388922496 38-39 22.370515165120768 26.965360593682814 28.62926825113494 22.034855990061484 40-41 22.454830969016868 26.914938148118406 28.650301410802115 21.979929472062615 42-43 22.191751108277124 27.06494570871934 28.307232185122132 22.436070997881405 44-45 22.381862903925047 27.04281913558556 28.167032505670125 22.408285454819264 46-47 22.547326295327036 27.1478582146706 27.802893233581344 22.501922256421018 48-49 22.488635362945192 27.06921261304751 27.962839853560478 22.479312170446818 50-51 22.358139701113014 27.26259030826285 28.046588836107944 22.332681154516195 52-53 22.542171693978236 27.405609794818048 27.691686338579107 22.360532172624605 54-55 22.28585607774443 27.150295794291562 27.83595409300571 22.727894034958297 56-57 22.267103107167433 27.429692400688875 27.846166128719535 22.457038363424157 58-59 21.598260237930127 26.827831479145186 29.268995971236805 22.304912311687882 60-61 21.710915335862236 27.00099446290617 28.956542794382823 22.331547406848774 62-63 22.513700548508247 27.082596591507475 27.97990824306394 22.42379461692034 64-65 22.34338425177968 27.282489250806314 27.882784588635616 22.491341908778388 66-67 22.063806247448596 26.86206543177081 28.52973255838786 22.544395762392732 68-69 21.434879765542846 26.478394618391633 29.966523684364923 22.1202019317006 70-71 22.29296538934844 26.368627475384233 28.94615412140098 22.392253013866352 72-73 21.615173820270446 26.493483383905513 29.258004768897255 22.633338026926786 74-75 21.63804087598521 26.97552741940598 28.768250076729824 22.618181627878982 76-77 20.702137642859714 27.113749666077027 29.031680774992175 23.15243191607109 78-79 21.954499150480874 27.044095223162667 28.163843646914238 22.837561979442217 80-81 22.010219217120586 27.35074906517924 27.645641174466583 22.993390543233588 82-83 22.24849047861398 27.313352066752206 27.517990175972667 22.92016727866115 84-85 22.186658286126946 27.178600932112158 27.380445096624424 23.254295685136476 86-87 22.1048317277116 27.19036180171867 27.533688726546153 23.17111774402358 88-89 22.30774210588222 27.09661075623601 27.294577264685493 23.301069873196273 90-91 21.944232430535248 27.023657093057047 27.755133032078767 23.276977444328942 92-93 21.789818487232438 27.34392793860112 27.636444744747575 23.229808829418868 94-95 20.739121270036858 27.321204314826332 28.450107557696242 23.489566857440565 96-97 20.314640135482335 27.20522263936852 28.56219358764133 23.917943637507815 98-99 21.181467521579535 27.063724205263505 27.931203024881807 23.82360524827515 100-101 20.426905121259857 27.201477870755998 28.401636563363645 23.969980444620496 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 590.0 1 439.5 2 314.0 3 364.5 4 399.5 5 443.0 6 576.0 7 873.5 8 1251.5 9 1558.5 10 1726.0 11 1826.0 12 2004.0 13 2229.0 14 2423.5 15 2634.5 16 2963.5 17 3322.5 18 3677.5 19 4169.0 20 4817.0 21 5725.5 22 6923.0 23 8644.0 24 10961.5 25 13900.5 26 17972.5 27 22727.0 28 27716.5 29 33299.0 30 39266.0 31 45828.5 32 52935.5 33 60327.5 34 67750.5 35 74949.5 36 82778.0 37 89669.5 38 94906.0 39 99573.5 40 103872.5 41 108312.5 42 112521.5 43 116472.5 44 120675.0 45 125094.5 46 130457.0 47 137522.0 48 144645.5 49 142257.5 50 132128.0 51 124133.0 52 116946.0 53 110241.5 54 103517.0 55 95103.0 56 86074.0 57 77130.5 58 67587.0 59 57349.5 60 46987.5 61 37027.0 62 28423.0 63 21354.0 64 15531.5 65 11132.5 66 7734.5 67 5269.5 68 3607.0 69 2434.5 70 1625.5 71 1051.0 72 671.0 73 446.5 74 287.0 75 178.0 76 106.5 77 60.0 78 27.5 79 17.0 80 12.0 81 5.0 82 3.5 83 3.0 84 2.0 85 1.5 86 2.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027201968997049727 2 0.01276516980867138 3 3.039326144921757E-4 4 0.0 5 0.0671083212798724 6 0.0 7 0.0 8 3.039326144921757E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.1030902956443725E-4 16-17 1.5196630724608785E-5 18-19 5.470787060859163E-4 20-21 0.0 22-23 0.0 24-25 1.5196630724608785E-4 26-27 1.5196630724608785E-4 28-29 0.0010941574121718326 30-31 0.005455590430134554 32-33 0.006093848920568123 34-35 0.001929972102025316 36-37 0.09174205968446324 38-39 0.08482759270476624 40-41 0.09102781804040663 42-43 0.09479658246010961 44-45 0.09841338057256649 46-47 0.09271464405083821 48-49 0.0821377890665105 50-51 0.07615031656101462 52-53 0.06373466925900925 54-55 0.051197448911207 56-57 0.06809610227697196 58-59 0.7087556603650291 60-61 0.3357239659680573 62-63 0.002963342991298713 64-65 0.002203511455068274 66-67 1.0900999117683619 68-69 2.3504476775445164 70-71 2.3192489946668946 72-73 1.7885826497635555 74-75 1.2701951824857007 76-77 0.7326447638641141 78-79 0.5577771341160409 80-81 0.03657829015413335 82-83 1.3676967652147907E-4 84-85 0.0012461237194179204 86-87 0.001352500134490182 88-89 0.0 90-91 0.32842958322024507 92-93 0.05087831966599022 94-95 0.9428597566776276 96-97 1.6431053038368757 98-99 0.6317543324834365 100-101 0.5613331457055993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3290203.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.97170781859858 #Duplication Level Percentage of deduplicated Percentage of total 1 69.90833223390428 34.93438752478182 2 15.276985201538187 15.268340816806417 3 6.054716204199494 9.076935272423741 4 2.977117687214954 5.950866208283506 5 1.6572663726684507 4.140821547628827 6 1.009282796708272 3.026135101406628 7 0.6726557278496502 2.3529628846226642 8 0.4446237479811324 1.7774886418658689 9 0.3260964929732097 1.466603880077423 >10 1.5524280258355336 13.155419499334279 >50 0.07966294599130873 2.685489090564293 >100 0.037300446070038566 3.31963925698327 >500 0.0019487542450710483 0.6673749777450262 >1k 0.0013397685404027474 1.3208120717369263 >5k 2.4359428007322676E-4 0.8567232257393196 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9504 0.2888575568133638 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6864 0.20861934658742942 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6689 0.20330052583381633 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5095 0.15485366708376352 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4467 0.1357666988936549 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3753 0.11406591021891355 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3620 0.11002360644616761 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.078652289843514E-5 4.558989217382635E-5 14-15 0.0 0.0 0.0 9.11797843476527E-5 6.078652289843514E-5 16-17 0.0 0.0 0.0 1.9755619941991422E-4 6.078652289843514E-5 18-19 1.5196630724608785E-5 0.0 0.0 2.4314609159374056E-4 1.2157304579687028E-4 20-21 3.039326144921757E-5 0.0 0.0 4.2550566028904595E-4 1.2157304579687028E-4 22-23 3.039326144921757E-5 0.0 0.0 6.686517518827865E-4 1.5196630724608785E-4 24-25 1.2157304579687028E-4 0.0 0.0 0.0010333708892733974 1.5196630724608785E-4 26-27 1.2157304579687028E-4 0.0 0.0 0.0013828933959393994 1.823595686953054E-4 28-29 1.3676967652147907E-4 0.0 0.0 0.0033584553901385415 1.823595686953054E-4 30-31 1.5196630724608785E-4 0.0 0.0 0.008844439081722312 1.823595686953054E-4 32-33 1.6716293797069663E-4 0.0 0.0 0.020773794200540208 1.823595686953054E-4 34-35 1.9755619941991422E-4 0.0 0.0 0.03777882398137744 1.823595686953054E-4 36-37 2.12752830144523E-4 0.0 0.0 0.06391702882770456 1.823595686953054E-4 38-39 2.7353935304295815E-4 0.0 0.0 0.10516068461429279 1.823595686953054E-4 40-41 2.7353935304295815E-4 0.0 0.0 0.15248299269072454 1.823595686953054E-4 42-43 2.7353935304295815E-4 0.0 0.0 0.20261667745120893 1.823595686953054E-4 44-45 3.039326144921757E-4 0.0 0.0 0.2617163743392125 1.823595686953054E-4 46-47 3.191292452167845E-4 0.0 0.0 0.3313929262115438 2.1275283014452297E-4 48-49 3.3432587594139327E-4 0.0 0.0 0.40187489951227934 3.3432587594139327E-4 50-51 3.3432587594139327E-4 0.0 0.0 0.47664232267735457 3.647191373906108E-4 52-53 3.3432587594139327E-4 0.0 0.0 0.5491150546030139 3.7991576811521963E-4 54-55 3.3432587594139327E-4 0.0 0.0 0.6285630400312685 4.25505660289046E-4 56-57 3.9511239883982844E-4 0.0 0.0 0.7061874297725703 4.25505660289046E-4 58-59 3.9511239883982844E-4 0.0 0.0 0.7661229413504274 4.25505660289046E-4 60-61 3.9511239883982844E-4 0.0 0.0 0.817062047539316 4.25505660289046E-4 62-63 4.5589892173826356E-4 0.0 0.0 0.8621048610070564 4.4070229101365475E-4 64-65 4.7109555246287237E-4 0.0 0.0 0.9146700066834782 4.5589892173826356E-4 66-67 4.862921831874811E-4 0.0 0.0 0.9581171739251346 4.5589892173826356E-4 68-69 4.862921831874811E-4 0.0 0.0 1.013220156932566 4.5589892173826356E-4 70-71 4.862921831874811E-4 0.0 0.0 1.0984428620361721 4.5589892173826356E-4 72-73 4.862921831874811E-4 1.5196630724608785E-5 0.0 1.2116273676730585 4.862921831874811E-4 74-75 4.862921831874811E-4 3.039326144921757E-5 0.0 1.3682894338130502 4.862921831874811E-4 76-77 4.862921831874811E-4 3.039326144921757E-5 0.0 1.5633685824248533 4.862921831874811E-4 78-79 4.862921831874811E-4 3.039326144921757E-5 0.0 1.8124413600011913 4.862921831874811E-4 80-81 4.862921831874811E-4 3.039326144921757E-5 0.0 2.113699367485836 5.014888139120899E-4 82-83 4.862921831874811E-4 3.039326144921757E-5 0.0 2.414896588447582 5.470787060859163E-4 84-85 5.166854446366987E-4 3.039326144921757E-5 0.0 2.6883903515983665 5.774719675351338E-4 86-87 5.470787060859163E-4 4.558989217382635E-5 0.0 2.943511388203099 5.774719675351338E-4 88-89 5.470787060859163E-4 6.078652289843514E-5 0.0 3.193860682760304 5.774719675351338E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 11885 0.0 23.628094 2 GTACATG 12285 0.0 23.478807 1 ACATGGG 12010 0.0 22.161583 3 GAGTACT 8310 0.0 20.319313 12-13 GTATCAA 22125 0.0 20.26984 1 AGTACTT 9120 0.0 19.342108 12-13 CATGGGA 7160 0.0 18.916185 4 ATCAACG 24545 0.0 18.130648 3 CATGGGG 5500 0.0 18.104412 4 TCAACGC 24655 0.0 18.049757 4 CAACGCA 24895 0.0 17.894705 5 AACGCAG 24975 0.0 17.864582 6 GTACTTT 9545 0.0 17.591429 14-15 TATCAAC 25485 0.0 17.463768 2 ACGCAGA 26885 0.0 16.38491 7 CGCAGAG 26755 0.0 16.30587 8 AGAGTAC 19055 0.0 16.175474 10-11 ATGGGAG 3610 0.0 16.079132 5 TACGCGG 140 1.0816677E-5 15.616455 64-65 TACTTTT 11195 0.0 15.567447 14-15 >>END_MODULE