FastQCFastQC Report
Fri 27 May 2016
SRR939607_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939607_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3203791
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82620.2578819904294631No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75530.23575195760272752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51170.15971703522483208No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43990.13730608519719295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35790.11171140689264687No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35630.111211998535485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG99150.026.5174482
GTACATG102200.026.0967561
ACATGGG98950.024.0290493
AGAGTAC118850.019.9233210-11
GAGTACT75500.019.6728812-13
GTATCAA196100.018.9731561
CATGGGG56800.018.7157654
GTACTTT80050.018.614414-15
CATGGGA50050.018.110784
CGTACGC800.001147270817.91395890-91
AGTACTT82250.017.7410712-13
CAACGCA218550.017.0898635
ATCAACG218300.016.9789943
AACGCAG222100.016.9193136
TCAACGC219400.016.8936044
TATCAAC219600.016.7704242
GAGTACA76200.015.6321921
AGTACAT68450.015.5999682
GTATAAT23200.015.54628851
ACGCAGA234500.015.5392937
Produced by FastQC (version 0.11.5)