##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939607_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3203791 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.157479685784747 31.0 30.0 33.0 26.0 34.0 2 30.302667683378846 31.0 30.0 34.0 26.0 34.0 3 30.346594393953914 31.0 30.0 34.0 26.0 34.0 4 33.9225311513766 35.0 35.0 37.0 28.0 37.0 5 33.915974856037735 35.0 35.0 37.0 28.0 37.0 6 34.01191650766233 36.0 35.0 37.0 29.0 37.0 7 33.82122398121476 35.0 35.0 37.0 28.0 37.0 8 33.8191401998445 35.0 35.0 37.0 28.0 37.0 9 35.098052276194046 38.0 35.0 39.0 28.0 39.0 10-11 34.980693341107454 37.0 34.0 39.0 27.5 39.0 12-13 34.83552095002452 37.0 34.0 39.0 27.0 39.0 14-15 35.52667745804892 38.0 34.0 40.0 27.0 41.0 16-17 35.256819811279826 38.0 33.5 40.0 27.0 41.0 18-19 35.2062266233971 38.0 33.5 40.0 26.5 41.0 20-21 35.3289559150394 38.0 34.0 40.0 26.5 41.0 22-23 35.185523806016064 38.0 34.0 40.0 26.0 41.0 24-25 35.05728198250136 38.0 34.0 40.0 25.0 41.0 26-27 35.18797995874263 38.0 34.0 40.0 25.5 41.0 28-29 35.07714548171214 38.0 34.0 40.0 25.0 41.0 30-31 34.87407714797875 38.0 34.0 40.0 25.0 41.0 32-33 34.65398133024283 38.0 33.0 40.0 24.0 41.0 34-35 34.42843571880937 38.0 33.0 40.0 24.0 41.0 36-37 34.15212852523776 38.0 33.0 40.0 23.5 41.0 38-39 33.99476807319828 38.0 33.0 40.0 23.0 41.0 40-41 33.73560556852804 38.0 32.5 40.0 21.0 41.0 42-43 33.42489741059889 38.0 32.0 40.0 19.0 41.0 44-45 33.1767259474791 37.0 31.0 40.0 18.5 41.0 46-47 32.767682255178315 37.0 31.0 40.0 15.5 41.0 48-49 32.42458340759431 37.0 30.5 40.0 14.0 41.0 50-51 31.818635641338652 36.5 30.0 38.5 12.5 40.5 52-53 31.88549908530238 36.5 30.0 39.0 9.0 40.0 54-55 31.928003418450203 37.0 30.0 39.0 9.0 40.0 56-57 31.771366328203058 36.0 30.0 39.0 8.0 40.0 58-59 31.366824334046758 36.0 29.5 39.0 7.5 40.0 60-61 30.93451429884159 35.5 29.0 39.0 4.0 40.0 62-63 30.521375301946975 35.0 28.0 38.5 2.0 40.0 64-65 29.904099549564876 34.5 28.0 38.0 2.0 40.0 66-67 29.352683274283496 34.0 27.0 38.0 2.0 40.0 68-69 28.815594400508648 34.0 26.0 37.0 2.0 39.0 70-71 28.251852570907403 33.5 26.0 36.5 2.0 39.0 72-73 27.50008318270449 32.5 25.0 36.0 2.0 39.0 74-75 27.032772580982964 32.0 24.0 35.5 2.0 38.0 76-77 26.637648492052072 32.0 23.5 35.0 2.0 37.5 78-79 26.040643256691837 31.5 21.5 35.0 2.0 37.0 80-81 25.348049701119706 31.0 19.5 34.5 2.0 36.0 82-83 24.66278106156113 31.0 17.5 34.0 2.0 36.0 84-85 23.980553818897675 30.0 11.5 34.0 2.0 35.0 86-87 23.30261961532447 30.0 6.5 34.0 2.0 35.0 88-89 22.474074463658834 29.0 2.0 34.0 2.0 35.0 90-91 21.17053468843629 28.0 2.0 33.5 2.0 35.0 92-93 19.451564100155096 25.0 2.0 32.0 2.0 34.0 94-95 19.20743066573319 25.0 2.0 32.0 2.0 34.0 96-97 18.585058763196475 25.0 2.0 32.0 2.0 34.0 98-99 17.58966065514261 23.5 2.0 32.0 2.0 34.0 100-101 15.960093682765198 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1130637742965561 1101 2 1.0333816019646171 1101 3 1.0125495166745324 1101 4 1.0964879721906655 1101 5 1.0123370012431039 1101 6 0.9413623533152915 1101 7 1.012631003073814 1101 8 0.9633920632813471 1101 9 1.2257778200125102 1101 10-11 0.9491529443667872 1101 12-13 1.0054319944541916 1101 14-15 1.1819945660811442 1101 16-17 1.0487931165675732 1101 18-19 1.1823555600315032 1101 20-21 1.2473077575522495 1101 22-23 1.4567819007890321 1101 24-25 1.3531694350406482 1101 26-27 1.3715144974151414 1101 28-29 1.3604810635902638 1101 30-31 1.4473706150300387 1101 32-33 1.3570782260578227 1101 34-35 1.4072552741614786 1101 36-37 1.286703128367705 1101 38-39 1.2003952836192084 1101 40-41 1.2799109174074061 1101 42-43 1.2391690461547213 1101 44-45 1.115097635772507 1101 46-47 1.1101979619532756 1101 48-49 1.0783593500622572 1101 50-51 1.3253968788018504 1101 52-53 1.3284598241682062 1101 54-55 1.4496269756320679 1101 56-57 1.2590046392967196 1101 58-59 1.192413893486357 1101 60-61 1.0315127468136254 1101 62-63 1.16804000794815 1101 64-65 0.9639943693615471 1101 66-67 0.8064816459387352 1101 68-69 0.7466681957259063 1101 70-71 0.7354687381844123 1101 72-73 0.7675728638071284 1101 74-75 0.6853953000800921 1101 76-77 0.045671579987963895 1101 78-79 0.2094705360540381 1101 80-81 0.0975746276673739 1101 82-83 -0.09717280896297353 1101 84-85 -0.12166089432369986 1101 86-87 -0.6041490114611214 1101 88-89 -1.0092439083263613 1101 90-91 -4.289666548406011 1101 92-93 -9.294670259156373 1101 94-95 -7.8842208006522725 1101 96-97 -4.602506945946745 1101 98-99 -3.3717275359339283 1101 100-101 -2.8802419137130677 1102 1 1.05415446943055 1102 2 0.9295696651175653 1102 3 0.8465577403324858 1102 4 1.0207712528053179 1102 5 0.9646500475770097 1102 6 0.9227767951168317 1102 7 0.9529054898933893 1102 8 0.8418985776681893 1102 9 1.1134849793384731 1102 10-11 0.9221314091471413 1102 12-13 0.8640119881142638 1102 14-15 1.0682913201764421 1102 16-17 1.279408454168582 1102 18-19 1.1933532772024336 1102 20-21 1.1923653528050622 1102 22-23 1.1992584852571255 1102 24-25 1.0516736232680088 1102 26-27 1.0400203504429086 1102 28-29 1.0818813887964893 1102 30-31 1.1578398150820348 1102 32-33 1.1421627545886253 1102 34-35 1.0696899649533052 1102 36-37 0.9180610125660138 1102 38-39 0.9637136869615759 1102 40-41 1.002228707282228 1102 42-43 0.9813045745920235 1102 44-45 0.7656680009567438 1102 46-47 0.7740716788596558 1102 48-49 0.7789524067042422 1102 50-51 0.8003434569528274 1102 52-53 0.9141295414486095 1102 54-55 1.0237743355550784 1102 56-57 0.9582407695707715 1102 58-59 0.8865712422118683 1102 60-61 0.9696097659086576 1102 62-63 0.7332261805263798 1102 64-65 0.8093287091769632 1102 66-67 0.999953917889588 1102 68-69 0.5786515203551446 1102 70-71 0.7657719827207892 1102 72-73 0.79114906035392 1102 74-75 0.6282314130219753 1102 76-77 0.526233210671375 1102 78-79 0.32381313373563003 1102 80-81 0.07504315039646414 1102 82-83 -0.1340178069218858 1102 84-85 -0.24306806117547097 1102 86-87 -0.7611436818192345 1102 88-89 -1.2581541482172618 1102 90-91 -5.501452309117177 1102 92-93 -10.546363452403213 1102 94-95 -9.21036227219901 1102 96-97 -5.738880326472231 1102 98-99 -4.215753981174537 1102 100-101 -3.265385508450475 1103 1 0.704154826861366 1103 2 0.7993248852065307 1103 3 0.770838467248943 1103 4 0.701021356554314 1103 5 0.6783731711310637 1103 6 0.619866405280213 1103 7 0.7314439891429672 1103 8 0.49157649487742816 1103 9 0.6264994116423921 1103 10-11 0.4712579234780421 1103 12-13 0.5643500800432193 1103 14-15 0.7059514726978122 1103 16-17 0.8454729565101289 1103 18-19 0.7547301684347332 1103 20-21 0.7247435055800153 1103 22-23 0.8027387515016997 1103 24-25 0.5925818624998342 1103 26-27 0.6134603436404333 1103 28-29 0.7786341674448494 1103 30-31 0.8435924722039161 1103 32-33 0.9024136823097137 1103 34-35 0.8451705494994215 1103 36-37 0.7962648625097799 1103 38-39 0.8195917496263476 1103 40-41 0.5614822713377805 1103 42-43 0.6507321434943449 1103 44-45 0.4556239504303292 1103 46-47 0.7324964549104465 1103 48-49 0.7163094578924714 1103 50-51 0.5867795782339975 1103 52-53 0.7907911714761084 1103 54-55 0.7922757071488071 1103 56-57 0.5953682561135025 1103 58-59 0.5450219225908377 1103 60-61 0.40605245262957723 1103 62-63 0.5402410989362103 1103 64-65 0.3816816423859315 1103 66-67 0.5184686461085981 1103 68-69 0.5643708423502716 1103 70-71 0.8397476518406819 1103 72-73 0.6489684426607951 1103 74-75 0.6040602480571167 1103 76-77 0.3538361441625284 1103 78-79 0.21284487998834933 1103 80-81 0.006382195348841435 1103 82-83 0.13874131819282454 1103 84-85 -0.1538037591456778 1103 86-87 -0.5177493795674586 1103 88-89 -0.8448688397902089 1103 90-91 -2.448163418857092 1103 92-93 -9.176063150317734 1103 94-95 -5.765252358460234 1103 96-97 -3.3530181450230394 1103 98-99 -2.741542708709538 1103 100-101 -2.3205009642481347 1104 1 0.8261608917118366 1104 2 0.8029225299275069 1104 3 0.7400322596796833 1104 4 0.8107631360845033 1104 5 0.7482444588888768 1104 6 0.6571600687473449 1104 7 0.9767516898831659 1104 8 0.8209830248906229 1104 9 0.9050002289239387 1104 10-11 0.78905458690258 1104 12-13 0.7511213431534074 1104 14-15 0.8888765201950974 1104 16-17 0.8959422737152138 1104 18-19 0.7596656272819402 1104 20-21 0.815339042336042 1104 22-23 0.8027393624554335 1104 24-25 0.643524668851235 1104 26-27 0.73698209743376 1104 28-29 0.7763240640854363 1104 30-31 0.8037041754940617 1104 32-33 0.7770389931106081 1104 34-35 0.6958587801564491 1104 36-37 0.6844906144541909 1104 38-39 0.39035660086434376 1104 40-41 0.5076957371770874 1104 42-43 0.5111894371298789 1104 44-45 0.38938809924608364 1104 46-47 0.23899333695167968 1104 48-49 0.19439280175748763 1104 50-51 0.17619021385629807 1104 52-53 0.16639653935055776 1104 54-55 0.29574854274918394 1104 56-57 0.3231105132349512 1104 58-59 0.10368796969222771 1104 60-61 0.13689669643293456 1104 62-63 0.02693862865823604 1104 64-65 0.18078338328988153 1104 66-67 0.07029454244755229 1104 68-69 0.03858946743205394 1104 70-71 0.041547940490140434 1104 72-73 0.09922860421790958 1104 74-75 0.0425152123878938 1104 76-77 -0.12727630833145298 1104 78-79 -0.28145308878249153 1104 80-81 -0.3278281766207378 1104 82-83 -0.23107936513973115 1104 84-85 -0.36355063577561353 1104 86-87 -0.6836914841948563 1104 88-89 -0.7033952577789222 1104 90-91 -1.3108638727782633 1104 92-93 -4.704951661385076 1104 94-95 -2.3240158963222584 1104 96-97 -1.3592483893975498 1104 98-99 -1.1140544686961888 1104 100-101 -0.8108700640933364 1105 1 0.895317897092653 1105 2 0.8309379539425308 1105 3 0.8107309218868011 1105 4 0.7207787813747117 1105 5 0.6573292590568158 1105 6 0.5943079876613311 1105 7 0.6893797653067963 1105 8 0.64749601168716 1105 9 0.8676729849134404 1105 10-11 0.6968424095335024 1105 12-13 0.7789503321882307 1105 14-15 0.9099504105035976 1105 16-17 0.8707610514676887 1105 18-19 0.7430081094930401 1105 20-21 0.6874778017340333 1105 22-23 0.7635893463917043 1105 24-25 0.749555286386304 1105 26-27 0.7126698412708592 1105 28-29 0.8092511588770321 1105 30-31 0.7211559435324233 1105 32-33 0.8197552098156109 1105 34-35 0.74518674543895 1105 36-37 0.8947246448752679 1105 38-39 0.9845870277623305 1105 40-41 0.8240241258611363 1105 42-43 0.9778658909681184 1105 44-45 0.74078913640259 1105 46-47 0.6075289278592635 1105 48-49 0.5239461946790911 1105 50-51 0.6831722614886182 1105 52-53 0.624161436595891 1105 54-55 0.8422512601077337 1105 56-57 0.6701540427443433 1105 58-59 0.6584645704089631 1105 60-61 0.43107256694156604 1105 62-63 0.5069076394943366 1105 64-65 0.5028035796148664 1105 66-67 0.4788372361466706 1105 68-69 0.2873729858351268 1105 70-71 0.23488660003061312 1105 72-73 0.37157739773624954 1105 74-75 0.2617396996074781 1105 76-77 0.10146052951851559 1105 78-79 0.0040120217238985845 1105 80-81 -0.0017346906926363204 1105 82-83 -0.023146915772386478 1105 84-85 -0.0671144073543779 1105 86-87 -0.25558792244454054 1105 88-89 -0.44102290767335717 1105 90-91 -1.6702581213256096 1105 92-93 -7.823732286189598 1105 94-95 -3.6364514020530727 1105 96-97 -1.6859123504077296 1105 98-99 -1.2194811007247779 1105 100-101 -0.9439227609200938 1106 1 0.7342153576568826 1106 2 0.783041129678292 1106 3 0.8380643996048285 1106 4 0.976221049042671 1106 5 0.7601784503626163 1106 6 0.7348474603661899 1106 7 0.7545031386705077 1106 8 0.7407939875592433 1106 9 0.8181535117272958 1106 10-11 0.6948131889813993 1106 12-13 0.8056886427641885 1106 14-15 0.7536717469238567 1106 16-17 0.9376050091081325 1106 18-19 0.6828883933153023 1106 20-21 0.7688648797515754 1106 22-23 0.9713284219826974 1106 24-25 0.7779558397490405 1106 26-27 0.6927195700458881 1106 28-29 0.7472071528845063 1106 30-31 0.7837734309813058 1106 32-33 0.8064292859914133 1106 34-35 0.6285955449585643 1106 36-37 0.8328906402650347 1106 38-39 0.821477792906073 1106 40-41 0.8100133540913674 1106 42-43 0.7741190801629969 1106 44-45 0.6297946616354935 1106 46-47 0.7306706045013414 1106 48-49 0.7153453159471255 1106 50-51 0.6799063972203037 1106 52-53 0.6794628732235992 1106 54-55 0.7425722362378089 1106 56-57 0.734234264671457 1106 58-59 0.592486641120523 1106 60-61 0.5637672778168081 1106 62-63 0.7598172988863681 1106 64-65 0.7067833366568408 1106 66-67 0.46998276080875456 1106 68-69 0.3119673515861834 1106 70-71 0.29653550682022 1106 72-73 0.4753204590163591 1106 74-75 0.40199278135327887 1106 76-77 0.28223408451033904 1106 78-79 0.21227463174027505 1106 80-81 -0.030786374257349536 1106 82-83 -0.018207895920607342 1106 84-85 -0.04625599557482829 1106 86-87 -0.14414490375231992 1106 88-89 -0.11292142090274382 1106 90-91 -0.5215385281546716 1106 92-93 -3.705649530255135 1106 94-95 -1.5056468435260655 1106 96-97 -0.42576386921719234 1106 98-99 0.016406974274676855 1106 100-101 0.17036555964526912 1107 1 0.5908338052647828 1107 2 0.5887477884241434 1107 3 0.5516332164367093 1107 4 0.6443901314118392 1107 5 0.4616868395581619 1107 6 0.40741043961570256 1107 7 0.42378106210165356 1107 8 0.39698082746086527 1107 9 0.4129572304185203 1107 10-11 0.33009700479760795 1107 12-13 0.41553131520633 1107 14-15 0.45492859177963396 1107 16-17 0.5336346182699714 1107 18-19 0.420097445842579 1107 20-21 0.43999942692791905 1107 22-23 0.5175395102471683 1107 24-25 0.5232509832809384 1107 26-27 0.32568943368217873 1107 28-29 0.3765427305706055 1107 30-31 0.36258942437867603 1107 32-33 0.30409432611428144 1107 34-35 0.16093360666784662 1107 36-37 0.26774257436150606 1107 38-39 0.219567033238512 1107 40-41 0.19744004636184798 1107 42-43 0.06511605797739861 1107 44-45 -0.15624381460133208 1107 46-47 0.06609315332323007 1107 48-49 0.08002592405236442 1107 50-51 0.046403303425865516 1107 52-53 0.1757699940657247 1107 54-55 0.1750116189841613 1107 56-57 0.08535212077566712 1107 58-59 -0.028026206091041672 1107 60-61 0.26067731043536924 1107 62-63 0.2272091055921699 1107 64-65 0.3014619682117896 1107 66-67 0.16798779681046838 1107 68-69 0.009131303327663431 1107 70-71 -0.0350013030495262 1107 72-73 -0.025173769895200593 1107 74-75 0.0020666005745546556 1107 76-77 -0.17302559437513665 1107 78-79 -0.2828645068053035 1107 80-81 -0.2892167174829332 1107 82-83 -0.18835467030744013 1107 84-85 -0.2616391010751151 1107 86-87 -0.43789101276144393 1107 88-89 -0.5629071630699798 1107 90-91 -0.9417982874174236 1107 92-93 -4.196162636866093 1107 94-95 -1.8557935503229146 1107 96-97 -1.0328915251381474 1107 98-99 -0.8883644035233651 1107 100-101 -0.7722327344566473 1108 1 0.43126132429518904 1108 2 0.31867298327731675 1108 3 0.15883933759719682 1108 4 -0.17353363805624866 1108 5 -0.7639809823045454 1108 6 -0.22452159818870854 1108 7 0.14389949726593443 1108 8 0.1862245807176066 1108 9 0.33405754775280627 1108 10-11 0.24327102998032757 1108 12-13 0.23618816408517773 1108 14-15 0.3093342585814014 1108 16-17 0.3990361005877574 1108 18-19 0.38662846657820893 1108 20-21 0.3992525747682052 1108 22-23 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58-59 0.41093444414371305 2311 60-61 0.557597674927397 2311 62-63 0.4414675888385915 2311 64-65 0.45016158856715904 2311 66-67 0.3652592219848039 2311 68-69 0.4269047483884165 2311 70-71 0.5586852730701892 2311 72-73 0.5124896459663688 2311 74-75 0.5742706270750197 2311 76-77 0.6232843800755816 2311 78-79 0.5385118985434545 2311 80-81 0.5040171123654851 2311 82-83 0.7185039915627449 2311 84-85 0.6798756132651071 2311 86-87 0.8424886802600646 2311 88-89 0.8740016295882 2311 90-91 1.7725873892237871 2311 92-93 2.8890880447245983 2311 94-95 2.076205335352345 2311 96-97 1.8865663683180287 2311 98-99 1.622924701532849 2311 100-101 1.6873712654991095 2312 1 0.21232300436026819 2312 2 -0.009552801440751324 2312 3 -0.07646399380159963 2312 4 -0.10932580417010485 2312 5 0.0035014502515267054 2312 6 0.026954836108956215 2312 7 -0.024724190464844753 2312 8 -0.5202920367960928 2312 9 -0.04722391739871057 2312 10-11 -0.1712265377760076 2312 12-13 -0.06397811661869213 2312 14-15 -0.34061340427953724 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2312 80-81 0.4570362751933992 2312 82-83 0.7385567705962934 2312 84-85 0.7936141034659201 2312 86-87 0.8348399984275297 2312 88-89 0.9645879196411613 2312 90-91 1.5477571935154515 2312 92-93 2.671367559098268 2312 94-95 1.9310825325993939 2312 96-97 1.5685692050748727 2312 98-99 1.4815602831196486 2312 100-101 1.6543696933869896 2313 1 -0.3147669753318141 2313 2 -0.9578670707896855 2313 3 -0.4782064824760326 2313 4 -0.12518541918754522 2313 5 -0.9197377492347059 2313 6 -0.33814922026721206 2313 7 -1.2148981963315748 2313 8 -1.1488631926116213 2313 9 -1.3194249831304035 2313 10-11 -2.05412588340981 2313 12-13 -0.6193641406063293 2313 14-15 -0.27279095101378914 2313 16-17 -1.6554450444007855 2313 18-19 -1.2104698806140703 2313 20-21 -1.4334498342931568 2313 22-23 -1.2375385494127116 2313 24-25 -0.5114796411963454 2313 26-27 -1.0398339842157824 2313 28-29 -2.626780032183781 2313 30-31 -0.74798243232609 2313 32-33 -0.5108199476520952 2313 34-35 -0.2223184145197692 2313 36-37 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-0.35772882548268825 2314 58-59 -0.44693757711136683 2314 60-61 -0.5522560643448102 2314 62-63 -0.5540114323324019 2314 64-65 -0.55473884600001 2314 66-67 -0.43269275948997077 2314 68-69 -0.39989360077063196 2314 70-71 -0.5299664637042838 2314 72-73 -0.3190184543016805 2314 74-75 -0.1130332599833821 2314 76-77 -0.15197088383032664 2314 78-79 -0.13751247558044355 2314 80-81 -0.0012116104879673628 2314 82-83 -0.03472503082445044 2314 84-85 -0.12819327671565972 2314 86-87 0.18370982233843947 2314 88-89 0.35301680642777455 2314 90-91 1.006124847968401 2314 92-93 1.8217033773773217 2314 94-95 1.1318590173587069 2314 96-97 0.8861122897595699 2314 98-99 0.7924060703256011 2314 100-101 0.9387307212789651 2315 1 -7.327008427869508 2315 2 -2.9974246555564434 2315 3 -1.5107958503531478 2315 4 -0.9225854333448353 2315 5 -1.1995161876858944 2315 6 -1.6562309078158748 2315 7 -3.0160693579370133 2315 8 -2.763202401219253 2315 9 -3.1203713977973564 2315 10-11 -2.18601552735403 2315 12-13 -2.8145439381571222 2315 14-15 -4.475767166770844 2315 16-17 -4.098469217969129 2315 18-19 -2.9123796986929236 2315 20-21 -4.00344434834426 2315 22-23 -5.573091745420356 2315 24-25 -3.5398209380038708 2315 26-27 -4.234171423162479 2315 28-29 -2.8196633407418616 2315 30-31 -2.717972345258765 2315 32-33 -1.7628074538459089 2315 34-35 -2.4194294430467096 2315 36-37 -3.035799880055883 2315 38-39 -2.1467767982099204 2315 40-41 -3.4977966638200257 2315 42-43 -7.629513264314589 2315 44-45 -4.157014277584892 2315 46-47 -2.7145312799239854 2315 48-49 -5.633283249215722 2315 50-51 -6.700716237924986 2315 52-53 -6.905264604231306 2315 54-55 -4.45536700752076 2315 56-57 -3.703239644698467 2315 58-59 -3.9031035553704463 2315 60-61 -2.817795315761586 2315 62-63 -3.2705279824248947 2315 64-65 -2.07707061258791 2315 66-67 -2.369390796901218 2315 68-69 -1.81250749621152 2315 70-71 -1.7298441378685325 2315 72-73 -2.1732213600195855 2315 74-75 -2.634670885238801 2315 76-77 -2.6151888291576455 2315 78-79 -1.6998856612985485 2315 80-81 -1.3753194758223977 2315 82-83 -1.6932317740652607 2315 84-85 -1.678159089269549 2315 86-87 -2.159955268982177 2315 88-89 -1.6457060021891792 2315 90-91 -0.7498350201268309 2315 92-93 0.030901341324973686 2315 94-95 0.0019404508519400565 2315 96-97 -0.13815676544133026 2315 98-99 -0.20310539393633675 2315 100-101 -0.1296402289958607 2316 1 -4.1106269902238495 2316 2 -2.729475410328032 2316 3 -4.343906408015869 2316 4 -4.07965675202448 2316 5 -3.679581382890696 2316 6 -4.177043486834595 2316 7 -2.826033713829716 2316 8 -1.4309220161410323 2316 9 -1.7003669042026317 2316 10-11 -3.1022699638267888 2316 12-13 -3.9375380050888324 2316 14-15 -3.0231669748410326 2316 16-17 -2.7554990280195497 2316 18-19 -3.080579709355696 2316 20-21 -5.460143803019886 2316 22-23 -6.4405653550716835 2316 24-25 -4.190908159439083 2316 26-27 -3.755634430976766 2316 28-29 -4.199983680731812 2316 30-31 -5.9638350012017405 2316 32-33 -4.169204162097365 2316 34-35 -5.729885428667206 2316 36-37 -8.907942829681925 2316 38-39 -5.841320317532375 2316 40-41 -5.066659479704505 2316 42-43 -4.113052998354711 2316 44-45 -3.217217403298914 2316 46-47 -5.657043732512754 2316 48-49 -3.22165721530812 2316 50-51 -4.425589617565194 2316 52-53 -5.182491599311206 2316 54-55 -6.910661261812525 2316 56-57 -5.035024401511215 2316 58-59 -5.840827663886952 2316 60-61 -6.26858321809064 2316 62-63 -6.3973360830814165 2316 64-65 -5.155806389218171 2316 66-67 -6.3997412211270195 2316 68-69 -5.109877410452405 2316 70-71 -6.149278554234357 2316 72-73 -8.067346365731783 2316 74-75 -7.870263773173118 2316 76-77 -7.176689956364459 2316 78-79 -6.0483374655910715 2316 80-81 -6.074199694866099 2316 82-83 -5.124696457457237 2316 84-85 -6.485584373736945 2316 86-87 -5.275065933272572 2316 88-89 -5.054339987474563 2316 90-91 -5.581826558459479 2316 92-93 -4.2240332349823575 2316 94-95 -4.401537896114503 2316 96-97 -4.992328953898124 2316 98-99 -5.933830538264502 2316 100-101 -4.932522793848332 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17913.0 3 7193.0 4 7271.0 5 8279.0 6 9455.0 7 10731.0 8 12943.0 9 16146.0 10 19557.0 11 22808.0 12 26382.0 13 29309.0 14 31756.0 15 35393.0 16 38664.0 17 43042.0 18 41497.0 19 41214.0 20 43836.0 21 48432.0 22 53106.0 23 59400.0 24 66949.0 25 74392.0 26 84605.0 27 95679.0 28 109013.0 29 123767.0 30 141323.0 31 161392.0 32 186542.0 33 216008.0 34 254367.0 35 304901.0 36 352715.0 37 309807.0 38 96319.0 39 1685.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43911843044931 19.342159275580457 13.725102451782526 23.493619842187705 2 15.050478509336642 20.81652304565077 40.56973713932931 23.56326130568328 3 19.244130119167494 25.305791790711602 29.98167014445501 25.468407945665895 4 12.389960608272263 17.006397406544625 38.848310604773 31.755331380410112 5 13.445293088306553 37.698220409754704 35.0844931240477 13.771993377891043 6 30.74864547849868 37.35602400888385 18.156819931809274 13.7385105808082 7 27.1339296509352 32.92314238610977 22.30386329703796 17.63906466591708 8 25.24671173379094 35.22249120041437 20.660149334442984 18.870647731351706 9 26.13489508149915 16.72726375336241 20.936030531060393 36.20181063407804 10-11 24.440717106730638 26.46861549016018 28.665243766428596 20.425423636680584 12-13 25.721563799301407 24.07696784660039 28.34486983594809 21.856598518150115 14-15 22.781338088398282 25.405668431070694 26.713545608555368 25.099447871975656 16-17 22.059860230219158 28.36855746957932 27.22728702009884 22.34429528010268 18-19 21.908805980501135 27.77497639654062 28.79437715922447 21.521840463733778 20-21 23.075190162970944 26.79069707857747 28.482256637562166 21.651856120889423 22-23 22.405128200320792 26.7571395046602 28.506724763271645 22.331007531747364 24-25 22.536297055681924 26.812133581603813 28.608713793779746 22.04285556893452 26-27 22.333162565050056 26.854041878967898 28.816741459840646 21.9960540961414 28-29 22.10339449809404 27.3865032439031 28.60929835015962 21.90080390784324 30-31 22.480603314893642 26.921429906559585 28.38114337762069 22.21682340092609 32-33 22.366504776608444 26.981051252370175 28.474303020244307 22.178140950777077 34-35 22.703211052328413 26.991995419345827 28.34934551590649 21.955448012419275 36-37 22.72609495661556 26.645009609557235 28.64271606813612 21.986179365691083 38-39 22.29191090086438 27.170682707703115 28.557688130522713 21.979718260909785 40-41 22.574059483854878 27.117839187263794 28.363964286060295 21.944137042821037 42-43 22.335344405952277 27.092929681212958 28.0742143612774 22.49751155155737 44-45 22.53651736393116 26.953350460133002 28.146002581832235 22.3641295941036 46-47 22.8241583040654 27.18443688831532 27.81966848811816 22.171736319501118 48-49 22.584437276037203 27.151926294963317 28.01775165501531 22.245884773984162 50-51 22.19111135010486 27.36517834699975 28.165670187794895 22.2780401151005 52-53 22.58457261231364 27.536889156111556 27.51483077973625 22.36370745183856 54-55 22.484662552710873 27.126549084130264 27.725164825287152 22.66362353787171 56-57 22.614745232600463 27.31006002920272 27.58606839900299 22.48912633919383 58-59 22.75610338098921 27.177131305845144 27.72072362504475 22.3460416881209 60-61 22.552500741857035 27.276696344594193 28.049384004734137 22.121418908814636 62-63 22.355140554925576 27.26372254565041 28.03777820384148 22.343358695582538 64-65 22.228254611692314 27.51604411626163 27.755364196009992 22.50033707603607 66-67 22.40659887039549 27.419754047103627 27.410925593795522 22.762721488705363 68-69 22.342834633685897 27.28701293810824 27.691845427708518 22.678307000497348 70-71 22.60447770056862 26.986871803975276 27.59948032270747 22.809170172748633 72-73 22.454636667399754 26.962560938648622 27.868694357263628 22.714108036688003 74-75 22.187659845347866 27.695453732549108 27.334070367595835 22.782816054507187 76-77 22.31188376687088 27.212154601467926 27.47981559984767 22.996146031813527 78-79 22.423087475368217 27.33265433898493 27.4376926281693 22.806565557477548 80-81 22.55767331542827 27.153981604135748 27.298752143612376 22.989592936823605 82-83 22.526151287478665 27.15287604362433 27.562626058437488 22.758346610459515 84-85 22.425862495299146 27.276846603209176 27.319392651256514 22.97789825023516 86-87 22.217645926983483 27.26339437763835 27.49985424456246 23.019105450815708 88-89 22.365964490662094 27.17621562872377 27.199463073146436 23.2583568074677 90-91 22.078416921142587 27.055488660271322 27.600994275171992 23.2651001434141 92-93 21.925924112745378 27.151445940004574 27.990741063277664 22.93188888397238 94-95 22.400639099007975 27.06729690667013 27.323327348294107 23.20873664602779 96-97 22.118196724361653 27.00088386667357 27.596059327420146 23.284860081544636 98-99 22.281394210545248 27.140989533569048 27.062925252512613 23.514691003373088 100-101 22.357575132959695 26.947196621454555 27.153366839577735 23.54186140600801 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2570.0 1 1501.5 2 423.5 3 414.5 4 401.0 5 390.5 6 515.0 7 948.5 8 1479.5 9 1726.0 10 1679.0 11 1587.5 12 1651.0 13 1779.5 14 1985.0 15 2302.5 16 2520.5 17 2735.0 18 3095.0 19 3537.0 20 4104.0 21 4879.5 22 6133.0 23 7808.5 24 9787.5 25 12633.5 26 17255.0 27 23026.5 28 28090.0 29 32862.0 30 37959.5 31 44279.0 32 51541.0 33 58658.5 34 65906.0 35 73237.0 36 81621.5 37 88914.0 38 93441.5 39 96859.5 40 100047.0 41 102188.0 42 103470.0 43 106142.5 44 110304.5 45 113619.5 46 117049.0 47 124439.5 48 134556.0 49 133535.5 50 121814.5 51 114508.5 52 110823.5 53 106733.0 54 102408.5 55 96022.0 56 88258.5 57 80386.5 58 72664.0 59 64613.5 60 54590.5 61 44340.0 62 35213.0 63 26820.5 64 19596.0 65 14299.5 66 10425.5 67 7509.5 68 5299.0 69 3720.5 70 2611.0 71 1698.5 72 1088.0 73 709.5 74 447.5 75 270.5 76 171.0 77 120.0 78 71.5 79 43.0 80 32.5 81 29.0 82 27.0 83 23.5 84 17.0 85 10.5 86 9.0 87 11.5 88 12.0 89 6.5 90 5.0 91 5.0 92 3.0 93 2.0 94 4.0 95 5.0 96 2.0 97 0.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08655371090061742 2 0.09744705569121082 3 0.09370149301249676 4 0.09735341662424296 5 0.09357664092320629 6 0.07728344327080013 7 0.09126687727133262 8 0.08702190623545668 9 0.08090415386022372 10-11 0.08792708388281258 12-13 0.08714675832474715 14-15 0.09343618232275451 16-17 0.09003396288958924 18-19 0.09329572372230273 20-21 0.10339313644366938 22-23 0.1124917324507123 24-25 0.13494950201183536 26-27 0.1412077129875201 28-29 0.13988115953880886 30-31 0.14148863018842364 32-33 0.13725926566370902 34-35 0.13259291882647778 36-37 0.1364633335944823 38-39 0.1299710249513779 40-41 0.12585090600479243 42-43 0.1250393674244044 44-45 0.116674277441943 46-47 0.13508996061228712 48-49 0.1522571228897266 50-51 0.1621516509659962 52-53 0.17040749537032845 54-55 0.16631858944606562 56-57 0.138320508422678 58-59 0.1295496491500226 60-61 0.12642834691776086 62-63 0.12349432281943484 64-65 0.10844964605993337 66-67 0.12204291728143316 68-69 0.11940541689517199 70-71 0.12837916081292444 72-73 0.15542524465547222 74-75 0.16814455125193872 76-77 0.17257680042175036 78-79 0.1695959567899404 80-81 0.17562007009820552 82-83 0.17546400498659245 84-85 0.1530686614701146 86-87 0.15417672376256752 88-89 0.17448079478343 90-91 0.265263870208762 92-93 0.538362209020501 94-95 0.17287332413381523 96-97 0.18448456843782882 98-99 0.20057488144513796 100-101 0.20836253051463097 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3203791.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.23275002376372 #Duplication Level Percentage of deduplicated Percentage of total 1 75.39342952914393 41.641864466174525 2 12.803089402889045 14.142996730433378 3 4.849930116762317 8.036249333155688 4 2.3749335201984034 5.246964377767024 5 1.2994611995341312 3.5886407799724407 6 0.8052481826950321 2.6685642949130837 7 0.5275581714980472 2.039694202654186 8 0.3572591857806139 1.5785925841531205 9 0.2684633380422522 1.3345171596569605 >10 1.2148611774209408 11.652923009318028 >50 0.07014820163995533 2.6714643010091845 >100 0.033330116892792506 3.2237406720814223 >500 0.0012011251898264644 0.45548142267257585 >1k 9.15143000261644E-4 1.057040392808592 >5k 1.7158931254905826E-4 0.6612662732298102 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8262 0.2578819904294631 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7553 0.23575195760272752 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5117 0.15971703522483208 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4399 0.13730608519719295 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3579 0.11171140689264687 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3563 0.111211998535485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.121302232261717E-5 2 0.0 0.0 0.0 0.0 3.121302232261717E-5 3 0.0 0.0 0.0 0.0 3.121302232261717E-5 4 0.0 0.0 0.0 3.121302232261717E-5 3.121302232261717E-5 5 3.121302232261717E-5 0.0 0.0 3.121302232261717E-5 3.121302232261717E-5 6 3.121302232261717E-5 0.0 0.0 6.242604464523434E-5 3.121302232261717E-5 7 3.121302232261717E-5 0.0 0.0 6.242604464523434E-5 3.121302232261717E-5 8 6.242604464523434E-5 0.0 0.0 6.242604464523434E-5 3.121302232261717E-5 9 6.242604464523434E-5 0.0 0.0 6.242604464523434E-5 3.121302232261717E-5 10-11 6.242604464523434E-5 0.0 0.0 1.092455781291601E-4 3.121302232261717E-5 12-13 9.363906696785153E-5 0.0 0.0 1.5606511161308587E-4 1.092455781291601E-4 14-15 1.5606511161308587E-4 0.0 0.0 1.7167162277439448E-4 1.5606511161308587E-4 16-17 1.7167162277439448E-4 0.0 0.0 2.340976674196288E-4 2.184911562583202E-4 18-19 2.0288464509701163E-4 0.0 0.0 3.1213022322617174E-4 2.184911562583202E-4 20-21 2.184911562583202E-4 0.0 0.0 3.589497567100975E-4 2.184911562583202E-4 22-23 2.184911562583202E-4 0.0 0.0 4.369823125166404E-4 2.184911562583202E-4 24-25 2.4970417858093737E-4 0.0 0.0 5.462278906458006E-4 2.340976674196288E-4 26-27 2.4970417858093737E-4 0.0 0.0 9.363906696785152E-4 2.65310689742246E-4 28-29 3.1213022322617174E-4 0.0 0.0 0.0028247785201968544 2.809172009035546E-4 30-31 3.433432455487889E-4 0.0 0.0 0.0077408295360090595 2.809172009035546E-4 32-33 3.433432455487889E-4 0.0 0.0 0.018462502703828057 3.1213022322617174E-4 34-35 4.369823125166404E-4 0.0 0.0 0.0343499310660402 3.433432455487889E-4 36-37 5.930474241297263E-4 0.0 0.0 0.05897700567858516 3.433432455487889E-4 38-39 6.866864910975778E-4 0.0 0.0 0.09657309106617754 3.433432455487889E-4 40-41 6.866864910975778E-4 0.0 0.0 0.13799277168829052 3.433432455487889E-4 42-43 7.491125357428122E-4 0.0 0.0 0.18165978991763196 3.433432455487889E-4 44-45 7.803255580654294E-4 0.0 0.0 0.2360484813147924 3.901627790327147E-4 46-47 8.271450915493552E-4 0.0 0.0 0.3017831063262242 4.5258882367794906E-4 48-49 8.427516027106637E-4 0.0 0.0 0.3723401432865003 5.618344018071092E-4 50-51 8.427516027106637E-4 0.0 0.0 0.4476415596398142 6.242604464523435E-4 52-53 8.427516027106637E-4 0.0 0.0 0.5244880205980977 6.554734687749606E-4 54-55 8.427516027106637E-4 0.0 0.0 0.6105891426750372 7.178995134201951E-4 56-57 8.427516027106637E-4 0.0 0.0 0.7155585367459987 7.178995134201951E-4 58-59 8.427516027106637E-4 0.0 0.0 0.8285964970873568 7.335060245815036E-4 60-61 8.427516027106637E-4 0.0 0.0 0.9490631567414978 7.803255580654294E-4 62-63 8.427516027106637E-4 0.0 0.0 1.074648752056548 9.05177647355898E-4 64-65 8.427516027106637E-4 0.0 0.0 1.2097699256911578 9.05177647355898E-4 66-67 8.427516027106637E-4 0.0 0.0 1.3669587061078579 9.05177647355898E-4 68-69 8.739646250332809E-4 0.0 0.0 1.546043421683874 9.05177647355898E-4 70-71 8.739646250332809E-4 0.0 0.0 1.7429975925395882 9.363906696785153E-4 72-73 8.739646250332809E-4 0.0 0.0 1.943603687007049 9.676036920011324E-4 74-75 8.739646250332809E-4 0.0 0.0 2.152215921700261 0.0010144232254850581 76-77 8.739646250332809E-4 0.0 0.0 2.3823807483072397 0.001092455781291601 78-79 8.739646250332809E-4 0.0 0.0 2.6484093375629056 0.001092455781291601 80-81 0.001061242758968984 0.0 0.0 2.937894513094019 0.0011392753147755267 82-83 0.0010768492701302926 0.0 0.0 3.2221983269195773 0.0011860948482594526 84-85 0.0011392753147755267 0.0 0.0 3.5086714457965575 0.0011860948482594526 86-87 0.0011860948482594526 0.0 0.0 3.8160104700962076 0.00121730787058207 88-89 0.0012173078705820697 0.0 0.0 4.157855490573511 0.0012797339152273042 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 9915 0.0 26.517448 2 GTACATG 10220 0.0 26.096756 1 ACATGGG 9895 0.0 24.029049 3 AGAGTAC 11885 0.0 19.92332 10-11 GAGTACT 7550 0.0 19.67288 12-13 GTATCAA 19610 0.0 18.973156 1 CATGGGG 5680 0.0 18.715765 4 GTACTTT 8005 0.0 18.6144 14-15 CATGGGA 5005 0.0 18.11078 4 CGTACGC 80 0.0011472708 17.913958 90-91 AGTACTT 8225 0.0 17.74107 12-13 CAACGCA 21855 0.0 17.089863 5 ATCAACG 21830 0.0 16.978994 3 AACGCAG 22210 0.0 16.919313 6 TCAACGC 21940 0.0 16.893604 4 TATCAAC 21960 0.0 16.770424 2 GAGTACA 7620 0.0 15.632192 1 AGTACAT 6845 0.0 15.599968 2 GTATAAT 2320 0.0 15.5462885 1 ACGCAGA 23450 0.0 15.539293 7 >>END_MODULE