FastQCFastQC Report
Fri 27 May 2016
SRR939607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939607_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3203791
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT91440.28541187611801144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78980.24652045030403047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60940.19021215803402908No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT48310.15079011084056357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43790.13668182475074062No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39000.12173078705820697No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35400.11049409902206479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95350.022.3083291
TACATGG92150.022.0568072
ACATGGG88200.021.6016223
AGAGTAC122250.020.11735510-11
GTATCAA184900.019.1140331
AGTACTT86500.018.84587912-13
GAGTACT80300.018.75061612-13
GTACTTT85350.017.53109214-15
AACGCAG207950.016.7854486
TCAACGC208000.016.736644
ATCAACG208500.016.6965073
CAACGCA210250.016.5799085
CATGGGG51300.016.5065024
GGTATCA146750.016.2905181
GAGTACA74650.016.2014031
TAGCCCT24200.016.1347644
GTATAGC15350.015.9420521
TATCAAC219000.015.9194592
CATGGGA45400.015.3357754
AGTACAT68750.015.3295552
Produced by FastQC (version 0.11.5)