##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939607_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3203791 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53383413587216 31.0 31.0 34.0 30.0 34.0 2 31.751111729822576 31.0 31.0 34.0 30.0 34.0 3 31.800348087624943 31.0 31.0 34.0 30.0 34.0 4 35.583672280744906 37.0 35.0 37.0 33.0 37.0 5 35.3139112382799 37.0 35.0 37.0 33.0 37.0 6 35.33033927618874 37.0 35.0 37.0 32.0 37.0 7 35.2634756761599 37.0 35.0 37.0 32.0 37.0 8 35.22552095314582 37.0 35.0 37.0 32.0 37.0 9 36.84797635051725 39.0 37.0 39.0 33.0 39.0 10-11 36.723650356718025 39.0 35.0 39.0 32.0 39.0 12-13 36.67133717524021 39.0 35.0 39.0 32.0 39.0 14-15 37.73899093292914 39.5 37.0 41.0 32.0 41.0 16-17 37.69676751698222 39.0 37.0 41.0 32.0 41.0 18-19 37.671003508031575 39.0 36.5 41.0 32.0 41.0 20-21 37.604288013793656 39.0 36.5 41.0 32.0 41.0 22-23 37.46210520598878 39.0 36.0 41.0 32.0 41.0 24-25 37.33605672155269 39.0 36.0 41.0 31.5 41.0 26-27 37.07028485940562 39.0 36.0 40.0 31.0 41.0 28-29 36.883258146364724 39.0 36.0 40.0 30.5 41.0 30-31 36.66038405751186 38.5 35.5 40.0 30.0 41.0 32-33 36.500485362497116 38.0 35.0 40.0 30.0 41.0 34-35 36.496190294560414 38.0 35.0 40.0 30.0 41.0 36-37 36.40771386148472 38.0 35.0 40.0 30.0 41.0 38-39 36.456154287217856 39.0 35.0 40.0 30.0 41.0 40-41 36.379628383998835 39.0 35.0 40.0 30.0 41.0 42-43 36.207698317399604 38.0 35.0 40.0 30.0 41.0 44-45 36.01466372182206 38.0 35.0 40.0 29.0 41.0 46-47 35.753686179903745 38.0 35.0 40.0 28.5 41.0 48-49 35.480465642109614 38.0 34.0 40.0 27.0 41.0 50-51 35.23634344437574 38.0 34.0 40.0 27.0 41.0 52-53 35.010231472652244 38.0 34.0 40.0 26.5 41.0 54-55 34.722786224195026 38.0 33.5 40.0 26.0 41.0 56-57 32.585708150125896 37.0 30.5 39.5 16.5 41.0 58-59 27.39889399776702 32.5 17.5 38.5 7.0 40.0 60-61 28.191616587973435 33.0 19.5 38.0 9.0 40.0 62-63 31.385454294615347 34.5 29.5 38.5 13.5 40.0 64-65 31.636173364617107 35.5 30.5 39.0 10.0 40.0 66-67 24.80684726313296 27.0 18.0 35.5 2.0 38.5 68-69 17.252407070248964 13.0 7.5 28.0 2.0 35.0 70-71 16.115276558302337 12.5 7.5 26.5 2.0 33.5 72-73 17.20693812424094 13.0 7.0 28.0 2.0 34.0 74-75 18.226105885184147 17.0 8.5 29.0 2.0 34.5 76-77 19.44791373719447 21.0 8.0 30.0 2.0 34.0 78-79 22.538785301538084 27.5 10.5 33.5 2.0 35.5 80-81 26.222422436419855 31.5 22.5 34.5 2.0 36.0 82-83 27.59043973217978 33.0 26.5 35.0 2.0 36.0 84-85 27.658313073480763 34.0 27.0 35.0 2.0 36.0 86-87 27.431181996578427 34.0 27.0 35.0 2.0 36.0 88-89 27.00167660749406 33.0 26.0 35.0 2.0 35.5 90-91 25.573695038159478 32.5 17.5 35.0 2.0 35.0 92-93 22.95813444135401 31.5 2.0 34.0 2.0 35.0 94-95 15.573751845860109 4.5 2.0 32.0 2.0 34.5 96-97 13.007887218610701 2.0 2.0 30.0 2.0 34.0 98-99 12.649933781573143 2.0 2.0 29.5 2.0 34.0 100-101 12.360901819126154 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.40174611176790975 1101 2 0.34413689770929423 1101 3 0.38948452298108904 1101 4 0.22928580389629616 1101 5 0.37690232030765713 1101 6 0.42297411843971133 1101 7 0.4280390589558394 1101 8 0.35493400716183743 1101 9 0.47388071122418296 1101 10-11 0.4254244545422381 1101 12-13 0.44974651131381194 1101 14-15 0.5761923822095909 1101 16-17 0.627718606242162 1101 18-19 0.5350987552464588 1101 20-21 0.5733515563886797 1101 22-23 0.623141881487598 1101 24-25 0.46826664226647097 1101 26-27 0.44213506688277704 1101 28-29 0.5509904744730179 1101 30-31 0.5568813294859325 1101 32-33 0.49487301977669773 1101 34-35 0.4441843140834578 1101 36-37 0.3110848143050404 1101 38-39 0.38372788483051323 1101 40-41 0.46777040007540194 1101 42-43 0.44597746528983606 1101 44-45 0.34156549708293227 1101 46-47 0.18885879796854255 1101 48-49 0.29571483940155474 1101 50-51 0.013820546320559401 1101 52-53 -0.033101749079342824 1101 54-55 -0.03696752319964958 1101 56-57 -2.2188168512459043 1101 58-59 -9.997666396588432 1101 60-61 -10.253876300411878 1101 62-63 -4.030399628190747 1101 64-65 -5.237542249705829 1101 66-67 -9.904529435279596 1101 68-69 -4.820499411706624 1101 70-71 -4.799619021476627 1101 72-73 -4.847911706311056 1101 74-75 -6.8045024452650225 1101 76-77 -8.269927822746471 1101 78-79 -11.37144646579691 1101 80-81 -8.418899895338935 1101 82-83 -4.552480011767347 1101 84-85 -3.6209093276042132 1101 86-87 -3.5431277900286986 1101 88-89 -3.6837406926191747 1101 90-91 -9.792964478356405 1101 92-93 -17.011202827066057 1101 94-95 -11.11987828698572 1101 96-97 -9.257088558889775 1101 98-99 -9.264093545654696 1101 100-101 -9.195742899624957 1102 1 0.334880429233948 1102 2 0.3189051369661975 1102 3 0.32079143692002887 1102 4 0.30190508228513835 1102 5 0.29936549515046806 1102 6 0.3455216735776787 1102 7 0.37807132871736115 1102 8 0.3192349848329883 1102 9 0.36957412340368023 1102 10-11 0.37841642016981325 1102 12-13 0.3104430820530055 1102 14-15 0.3884789513752054 1102 16-17 0.3956489628515385 1102 18-19 0.4091211411107025 1102 20-21 0.5458562372145153 1102 22-23 0.5296634520557504 1102 24-25 0.4891532739191149 1102 26-27 0.44639912247343005 1102 28-29 0.49003608772391516 1102 30-31 0.5623673029722767 1102 32-33 0.4738918593880044 1102 34-35 0.2551051767693622 1102 36-37 0.20085484379253415 1102 38-39 0.23991603920540427 1102 40-41 0.3760291784767773 1102 42-43 0.40937044256873634 1102 44-45 0.4688022020552083 1102 46-47 0.3726707804265672 1102 48-49 0.30573032432598524 1102 50-51 0.2822328968591137 1102 52-53 0.18712124295471 1102 54-55 0.1861709220591976 1102 56-57 -2.4522174227513673 1102 58-59 -10.138280343054834 1102 60-61 -10.51972622663613 1102 62-63 -4.142772972227455 1102 64-65 -5.090312774072348 1102 66-67 -6.732932070681461 1102 68-69 -4.199941407095356 1102 70-71 -4.094559168480785 1102 72-73 -4.255136484014589 1102 74-75 -5.191334672152234 1102 76-77 -7.364929772615454 1102 78-79 -9.940945383676638 1102 80-81 -5.780879274619242 1102 82-83 -3.192056992822831 1102 84-85 -2.783974704088564 1102 86-87 -2.6984414680745417 1102 88-89 -2.7662198084961247 1102 90-91 -8.714773591884388 1102 92-93 -13.229133981867413 1102 94-95 -10.334563537082074 1102 96-97 -8.980219364311095 1102 98-99 -9.181082491836031 1102 100-101 -9.005117664443391 1103 1 0.2665723900881254 1103 2 0.23402171396789484 1103 3 0.27852712397918467 1103 4 0.2177323822489825 1103 5 0.20355240212100512 1103 6 0.22871610427880285 1103 7 0.18439932153262362 1103 8 0.23714083906065753 1103 9 0.3949872663982106 1103 10-11 0.34863321512905543 1103 12-13 0.3314248353978968 1103 14-15 0.48048484183507867 1103 16-17 0.4521776812889797 1103 18-19 0.3587229255558455 1103 20-21 0.27234754205145606 1103 22-23 0.4625783906698331 1103 24-25 0.4855453860605863 1103 26-27 0.5107771157213747 1103 28-29 0.4571521703423542 1103 30-31 0.48781118501710097 1103 32-33 0.3714554792516793 1103 34-35 0.36189685141550854 1103 36-37 0.2616558951658803 1103 38-39 0.274737984103119 1103 40-41 0.38254937501105957 1103 42-43 0.2981983591947923 1103 44-45 0.2732324253516438 1103 46-47 0.4090790597282776 1103 48-49 0.28534378735277954 1103 50-51 0.3451268937897254 1103 52-53 0.527527536446037 1103 54-55 0.364641114398367 1103 56-57 -2.7151834137734525 1103 58-59 -10.214362878721726 1103 60-61 -10.691852210088214 1103 62-63 -4.574594642844044 1103 64-65 -5.872892499016167 1103 66-67 -6.897056683104179 1103 68-69 -4.289644596544601 1103 70-71 -4.459321268172378 1103 72-73 -4.662284517185672 1103 74-75 -5.438328773038775 1103 76-77 -7.029863683657318 1103 78-79 -9.769032918107573 1103 80-81 -5.722943689672423 1103 82-83 -3.4396705777523415 1103 84-85 -2.8976612192020568 1103 86-87 -2.770390880702834 1103 88-89 -2.7424770902084212 1103 90-91 -5.762818783931969 1103 92-93 -10.609724058760186 1103 94-95 -8.751720667808069 1103 96-97 -8.279668506985061 1103 98-99 -8.479058831420893 1103 100-101 -8.457172364903197 1104 1 0.22630669852689778 1104 2 0.1926725395933211 1104 3 0.1275409050007994 1104 4 0.19724937269785414 1104 5 0.2517179000622676 1104 6 0.21024033959164967 1104 7 0.17524513985104306 1104 8 0.21457168433435214 1104 9 0.25545328794427746 1104 10-11 0.16655852795483383 1104 12-13 0.2925441759588736 1104 14-15 0.3678915666552314 1104 16-17 0.28044216420916257 1104 18-19 0.3085457315162401 1104 20-21 0.31682881439926547 1104 22-23 0.444878711787112 1104 24-25 0.3803532044530229 1104 26-27 0.4030084549249793 1104 28-29 0.4224355970177882 1104 30-31 0.38124427022174956 1104 32-33 0.44094073152669466 1104 34-35 0.4097877692914551 1104 36-37 0.3181977434021803 1104 38-39 0.3421811671511463 1104 40-41 0.13710394784268232 1104 42-43 0.24303700456722055 1104 44-45 0.27010408037993017 1104 46-47 0.31189831447643 1104 48-49 0.28008757779355875 1104 50-51 0.3178939800710552 1104 52-53 0.23032315601248143 1104 54-55 0.21928858710967347 1104 56-57 -3.1249225051072003 1104 58-59 -10.605344119299946 1104 60-61 -10.722049471339712 1104 62-63 -4.342736739511587 1104 64-65 -5.511931430384767 1104 66-67 -6.884894339778899 1104 68-69 -4.210480091490442 1104 70-71 -4.108908490371714 1104 72-73 -4.3534460065721845 1104 74-75 -5.123739180338939 1104 76-77 -6.931141589405659 1104 78-79 -9.60208562002429 1104 80-81 -5.1637791916005895 1104 82-83 -2.7684596080954442 1104 84-85 -2.2668397028777214 1104 86-87 -2.188736302774114 1104 88-89 -2.16940754691554 1104 90-91 -4.607406816081401 1104 92-93 -6.972859075346708 1104 94-95 -7.893412447582637 1104 96-97 -7.815410958913476 1104 98-99 -8.15146081174888 1104 100-101 -8.10289286089988 1105 1 0.3130877048892664 1105 2 0.24040929162823588 1105 3 0.2845799735457497 1105 4 0.21207587380528992 1105 5 0.22249822841478561 1105 6 0.1182886215529706 1105 7 0.1808713450738466 1105 8 0.20086733918478217 1105 9 0.2950493117994455 1105 10-11 0.3350519560109575 1105 12-13 0.26795080456904685 1105 14-15 0.4308038423924785 1105 16-17 0.4767346169523705 1105 18-19 0.4417965653761584 1105 20-21 0.3848643730375514 1105 22-23 0.4545758130112887 1105 24-25 0.35450203967321414 1105 26-27 0.4846335544810856 1105 28-29 0.5889638780429394 1105 30-31 0.4788349593591974 1105 32-33 0.4401725626710302 1105 34-35 0.43430729363491594 1105 36-37 0.34204351318094695 1105 38-39 0.27653584148301036 1105 40-41 0.21193875386165217 1105 42-43 0.2783015844387009 1105 44-45 0.42799057259381357 1105 46-47 0.5427092525626449 1105 48-49 0.36472010496181895 1105 50-51 0.5224743231752456 1105 52-53 0.462349689176456 1105 54-55 0.3898479594257438 1105 56-57 1.4538725593214679 1105 58-59 -8.808835019003425 1105 60-61 -10.364374006147646 1105 62-63 -3.945111529317895 1105 64-65 -4.947583614165154 1105 66-67 -6.473303527237988 1105 68-69 -3.8451087534351522 1105 70-71 -3.7934871880963374 1105 72-73 -2.084862791855116 1105 74-75 -4.336116991402555 1105 76-77 -6.444489553851277 1105 78-79 -9.161671764509894 1105 80-81 -4.733817308378569 1105 82-83 -2.285466857694562 1105 84-85 -1.7225748258450224 1105 86-87 -1.622542310395339 1105 88-89 -1.6360336464652612 1105 90-91 -4.382891227883029 1105 92-93 -6.884853076803436 1105 94-95 -8.024902299775071 1105 96-97 -8.279071563207491 1105 98-99 -8.632762260888567 1105 100-101 -8.5196776119192 1106 1 0.1674041077180881 1106 2 0.18315768227054008 1106 3 0.13725973668769953 1106 4 0.1336545581473345 1106 5 0.033926780656017286 1106 6 0.17135335458399226 1106 7 0.1626939835866139 1106 8 0.21594238844626545 1106 9 0.20531002690661637 1106 10-11 0.2783163260655783 1106 12-13 0.2849686114550778 1106 14-15 0.36077600636647844 1106 16-17 0.32587191840243435 1106 18-19 0.252996788332986 1106 20-21 0.25973027155525585 1106 22-23 0.2609903549004784 1106 24-25 0.23441650573246164 1106 26-27 0.24247425898884956 1106 28-29 0.40127734616890365 1106 30-31 0.34510339289159475 1106 32-33 0.2403775833409938 1106 34-35 0.4370333941904647 1106 36-37 0.3907864989109342 1106 38-39 0.34482994191319705 1106 40-41 0.20421585159497369 1106 42-43 0.12918311455829468 1106 44-45 0.21580103466401113 1106 46-47 0.10556654172321345 1106 48-49 0.1942783101817227 1106 50-51 0.13258164353522517 1106 52-53 0.204758579915854 1106 54-55 0.16799206206647455 1106 56-57 1.565503334079672 1106 58-59 -8.709262324947318 1106 60-61 -10.268654088950587 1106 62-63 -4.178524498085473 1106 64-65 -5.576421490859506 1106 66-67 -7.077132631722698 1106 68-69 -3.897746768134411 1106 70-71 -4.337585939392582 1106 72-73 -4.9141378239919025 1106 74-75 -5.133373738169151 1106 76-77 -6.75164378748474 1106 78-79 -9.153849108088945 1106 80-81 -5.053567287812147 1106 82-83 -2.7331446145608105 1106 84-85 -2.1792434680077015 1106 86-87 -2.1237166952074134 1106 88-89 -2.134284101305031 1106 90-91 -4.6468313462896695 1106 92-93 -6.777050734973232 1106 94-95 -7.550911971311083 1106 96-97 -7.503894342187541 1106 98-99 -7.10007395778368 1106 100-101 -7.582029274833543 1107 1 0.01317719462930711 1107 2 -0.052913849121168255 1107 3 -0.05926562672719626 1107 4 -0.01606382910453874 1107 5 -0.1407070095125249 1107 6 -0.014178772213355728 1107 7 0.06174587880972382 1107 8 0.07768132524488891 1107 9 0.015436522471979686 1107 10-11 0.02036121348623965 1107 12-13 0.03712578227725061 1107 14-15 0.0633572495252821 1107 16-17 -0.0287130754523659 1107 18-19 -0.0691704972387015 1107 20-21 0.09292120923655744 1107 22-23 0.05821511022474368 1107 24-25 0.021444307501646165 1107 26-27 -0.04586848761959317 1107 28-29 0.0022792489182421605 1107 30-31 0.05192983496953474 1107 32-33 0.1277828568940933 1107 34-35 0.048251888124895004 1107 36-37 0.0627934135488033 1107 38-39 -0.06796381876419133 1107 40-41 -0.034804475452858696 1107 42-43 -0.17511429305533 1107 44-45 0.018229076907381625 1107 46-47 -0.04950908233326601 1107 48-49 -0.21425476981705316 1107 50-51 -0.2595747947576825 1107 52-53 -0.37645758855784095 1107 54-55 -0.3038958942024408 1107 56-57 0.9310912920292012 1107 58-59 -8.880143683703253 1107 60-61 -10.494728696599875 1107 62-63 -4.152783903688206 1107 64-65 -5.986626783557011 1107 66-67 -7.271513839953915 1107 68-69 -4.358749129399579 1107 70-71 -4.107513566962281 1107 72-73 -2.667788368072994 1107 74-75 -5.037224933160038 1107 76-77 -7.069472709567009 1107 78-79 -7.786200751731176 1107 80-81 -2.924723975274606 1107 82-83 -1.765433627863981 1107 84-85 -1.5424219800927936 1107 86-87 -1.4666186236044112 1107 88-89 -1.3554990805603886 1107 90-91 -1.0072633303077438 1107 92-93 -3.9103279960225485 1107 94-95 -6.510108538324541 1107 96-97 -6.919869261919871 1107 98-99 -6.497101441697264 1107 100-101 -6.850704980655635 1108 1 -0.10116360612281028 1108 2 -0.14778225602587725 1108 3 -0.11477431275373462 1108 4 -0.12681032285021132 1108 5 -0.0928340934834182 1108 6 -0.24525209821298688 1108 7 -0.27397831744678314 1108 8 -0.1625234601340253 1108 9 -0.09261962584526628 1108 10-11 -0.06688133150161946 1108 12-13 -0.11192543715761616 1108 14-15 -0.2147000222821447 1108 16-17 -0.19050824044688852 1108 18-19 -0.11772422763966262 1108 20-21 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100-101 12.030715995407716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 17.0 4 42.0 5 113.0 6 269.0 7 658.0 8 1511.0 9 2599.0 10 4288.0 11 6743.0 12 10135.0 13 14323.0 14 17566.0 15 24479.0 16 33926.0 17 45337.0 18 52177.0 19 54097.0 20 57174.0 21 57449.0 22 57785.0 23 48641.0 24 48918.0 25 57049.0 26 81048.0 27 121234.0 28 172977.0 29 233577.0 30 288524.0 31 312139.0 32 309518.0 33 281680.0 34 247900.0 35 221343.0 36 178901.0 37 114548.0 38 42227.0 39 2877.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.54844732228853 20.067311579870843 14.535492340810144 23.848748757030485 2 15.329595001573246 21.51632180119167 39.4310656118428 23.723017585392288 3 18.938487815048934 25.373807505365527 30.559637529074955 25.128067150510585 4 12.733102752333094 17.68838853720483 38.370761388617424 31.207747321844653 5 13.186774716450586 38.02075226004965 35.16481592965386 13.627657093845901 6 30.790366787346617 37.787889409764865 17.77868781078416 13.643055992104353 7 26.955191521544318 32.97724477033614 22.195985942903267 17.87157776521627 8 25.199508699974558 35.57299348106669 20.471167336975945 18.756330481982804 9 25.599766027184668 16.629611606999333 20.81758766411417 36.953034701701824 10-11 24.33130001301583 26.583662916838207 28.63746417915526 20.447572890990703 12-13 25.388531898616357 24.11327705209235 28.458832052402922 22.039358996888375 14-15 22.38132463566768 25.533703012215447 26.703901948063404 25.381070404053474 16-17 21.820134334605473 28.434907270792632 27.29592847972917 22.449029914872725 18-19 21.653636516154705 27.88813240578534 28.83711856669645 21.621112511363503 20-21 22.81333270491115 26.856792468672268 28.496724661502576 21.833150164914002 22-23 22.153000182128583 26.851246519541522 28.559650075902514 22.436103222427377 24-25 22.319998763964122 26.928134033732853 28.63203758173326 22.11982962056976 26-27 22.193001553632307 26.94060848387804 28.77437841051944 22.092011551970213 28-29 21.993174719806422 27.427444331171856 28.59939419629563 21.979986752726084 30-31 22.38341888039358 26.99165964938842 28.327426853166283 22.297494617051715 32-33 22.253023375442922 27.011999235884467 28.486245920347198 22.248731468325413 34-35 22.536316739622432 27.02679924654773 28.414823887789364 22.022060126040472 36-37 22.526923283640667 26.702438344555535 28.690874081777284 22.07976429002651 38-39 22.17595977776528 27.192989856947335 28.57089512596009 22.060155239327297 40-41 22.458573874019574 27.144380664865004 28.406075649508683 21.990969811606735 42-43 22.20711333685029 27.134847999959998 28.07173779336795 22.58630086982176 44-45 22.446912853748284 26.92592620950346 28.185123301623054 22.4420376351252 46-47 22.64094281820169 27.191130931538744 27.90105390030298 22.266872349956586 48-49 22.45529481174822 27.162745509794146 28.064270973564376 22.31768870489326 50-51 22.139520552878665 27.33996047340048 28.157975778122314 22.362543195598544 52-53 22.52081772209202 27.505261544820485 27.573587818240863 22.400332914846626 54-55 22.4167014710297 27.117633332469083 27.752218133859298 22.71344706264192 56-57 21.590960993252324 27.133139576951454 28.79468661550649 22.481212814289734 58-59 21.319955307900496 26.66553006844172 29.928402241583157 22.08611238207463 60-61 22.312182567499807 27.09059091317011 28.520617854728957 22.076608664601128 62-63 22.329073368739046 27.233781346511734 28.114314841994663 22.322830442754555 64-65 21.782167388033454 27.44417396119823 28.252482906505772 22.521175744262546 66-67 21.889717102302043 26.77609138741312 28.902298765539342 22.431892744745497 68-69 22.101438911581067 26.145487199050987 29.604679363216302 22.14839452615164 70-71 22.023861983783252 26.12413811703163 29.5164626048222 22.335537294362922 72-73 21.27239458226034 26.29269227100881 30.02931042359453 22.40560272313632 74-75 21.65975879574978 26.849501212646693 29.027232470773207 22.46350752083032 76-77 19.973856979056134 27.227975312324553 29.593355062186255 23.204812646433055 78-79 20.851918035829467 27.23396920718959 28.918290750403415 22.995822006577527 80-81 22.093353711894036 27.138983741081518 27.710865949336334 23.056796597688113 82-83 22.40990330172484 27.08779941194464 27.63903888531672 22.863258401013802 84-85 22.305542086242067 27.187783697686285 27.39062193477745 23.1160522812942 86-87 22.076498434036314 27.201135659212344 27.612072372055742 23.1102935346956 88-89 22.214094678994385 27.165521640273134 27.24238388767531 23.377999793057178 90-91 21.583005088929134 27.047333690588417 27.899316651867963 23.47034456861449 92-93 20.707717581107556 27.30323402324325 28.665540773462933 23.32350762218626 94-95 19.89916497488044 27.058869063869086 29.40836273850522 23.633603222745254 96-97 19.792847655340264 27.057511535833385 29.2210561269799 23.92858468184645 98-99 20.149423660192443 27.210849129354298 28.582255061519845 24.057472148933414 100-101 20.51602879992185 26.977733554240928 28.3014301482145 24.20480749762272 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 293.0 1 228.5 2 165.0 3 172.5 4 204.0 5 264.0 6 336.5 7 486.0 8 670.0 9 837.0 10 976.0 11 1154.0 12 1428.0 13 1680.5 14 1974.5 15 2345.0 16 2606.5 17 2862.5 18 3304.5 19 3867.5 20 4573.5 21 5494.0 22 6799.0 23 8569.0 24 10807.5 25 13828.5 26 17924.5 27 22794.5 28 28004.0 29 33649.5 30 39691.0 31 46735.5 32 54395.0 33 62369.0 34 70242.5 35 77601.0 36 84933.5 37 91615.0 38 97296.0 39 101847.5 40 105794.0 41 109097.0 42 112303.5 43 115632.5 44 119531.0 45 123550.5 46 127401.0 47 131644.0 48 133502.0 49 130363.0 50 124323.0 51 118358.5 52 112579.5 53 106578.5 54 99923.5 55 91931.5 56 82807.5 57 73153.5 58 63419.0 59 53556.5 60 43304.5 61 33812.5 62 25646.0 63 18777.0 64 13445.0 65 9449.0 66 6567.5 67 4551.5 68 3016.0 69 1954.5 70 1251.0 71 742.5 72 416.5 73 246.0 74 132.5 75 67.5 76 38.5 77 20.0 78 10.5 79 4.0 80 2.0 81 1.5 82 1.0 83 1.0 84 1.0 85 0.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02422130532235093 2 0.00696050397794363 3 9.363906696785151E-5 4 0.0 5 0.14123892600984272 6 0.0 7 0.0 8 0.0014357990268403901 9 0.0 10-11 0.0 12-13 0.0 14-15 6.08653935291035E-4 16-17 0.0 18-19 1.0924557812916012E-4 20-21 0.0 22-23 3.277367343874803E-4 24-25 1.560651116130859E-5 26-27 2.65310689742246E-4 28-29 0.0035270715224557407 30-31 0.012126259172336772 32-33 0.0027155329420676944 34-35 0.0042917905693598615 36-37 0.1119611110712278 38-39 0.11146170271406593 40-41 0.12386887908730625 42-43 0.12656880551821265 44-45 0.12280763632833729 46-47 0.11628411466291029 48-49 0.11595637792852281 50-51 0.1144737593681985 52-53 0.09278070885397956 54-55 0.08003018923519044 56-57 0.5673122872247285 58-59 1.5952507513754801 60-61 0.527281586095972 62-63 0.005150148683231833 64-65 0.12368160095337057 66-67 1.9065694360212635 68-69 2.882272907315115 70-71 2.7833120200412576 72-73 2.640418803848316 74-75 2.009400738063126 76-77 1.428869735884769 78-79 0.9444436294377505 80-81 0.05409216768509556 82-83 3.433432455487889E-4 84-85 2.0288464509701163E-4 86-87 4.0576929019402327E-4 88-89 2.340976674196288E-4 90-91 0.2537150519493937 92-93 0.4008688456893724 94-95 2.01648609413036 96-97 2.430838965463103 98-99 1.4417919271263326 100-101 0.9525746217527923 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3203791.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.427204558656626 #Duplication Level Percentage of deduplicated Percentage of total 1 70.535647042455 34.86379855044626 2 14.923946065981669 14.752978700512696 3 5.830554068113882 8.645639658449172 4 2.8704359917719926 5.675105077513788 5 1.6615957643659154 4.106401686955576 6 1.0107659809796914 2.9975602141688658 7 0.6516436543545462 2.254624694219288 8 0.4702839778903731 1.8595857900676975 9 0.34908296795822874 1.5528775738692913 >10 1.552040554523624 13.408502955769464 >50 0.0945210075075202 3.2455247435000203 >100 0.04651411166277334 4.084028960247419 >500 0.0015791555510184753 0.5376619689510271 >1k 0.0012001582176151605 1.293373480587516 >5k 1.8949866593923585E-4 0.7223359447417749 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9144 0.28541187611801144 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7898 0.24652045030403047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6094 0.19021215803402908 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4831 0.15079011084056357 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4379 0.13668182475074062 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3900 0.12173078705820697 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3540 0.11049409902206479 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.121302232261717E-5 0.0 0.0 0.0 3.121302232261717E-5 7 3.121302232261717E-5 0.0 0.0 0.0 3.121302232261717E-5 8 3.121302232261717E-5 0.0 0.0 0.0 3.121302232261717E-5 9 3.121302232261717E-5 0.0 0.0 6.242604464523434E-5 6.242604464523434E-5 10-11 3.121302232261717E-5 0.0 0.0 1.092455781291601E-4 9.363906696785153E-5 12-13 3.121302232261717E-5 0.0 0.0 1.7167162277439448E-4 1.5606511161308587E-4 14-15 3.121302232261717E-5 0.0 0.0 2.0288464509701163E-4 1.8727813393570306E-4 16-17 3.121302232261717E-5 0.0 0.0 2.65310689742246E-4 1.8727813393570306E-4 18-19 3.121302232261717E-5 0.0 0.0 3.433432455487889E-4 1.8727813393570306E-4 20-21 3.121302232261717E-5 0.0 0.0 3.901627790327147E-4 1.8727813393570306E-4 22-23 3.121302232261717E-5 0.0 0.0 4.681953348392576E-4 2.0288464509701163E-4 24-25 1.5606511161308587E-4 0.0 0.0 5.774409129684177E-4 2.184911562583202E-4 26-27 1.5606511161308587E-4 0.0 0.0 9.363906696785153E-4 2.65310689742246E-4 28-29 2.184911562583202E-4 0.0 0.0 0.0027467459643903116 2.809172009035546E-4 30-31 2.4970417858093737E-4 0.0 0.0 0.007834468602976912 2.809172009035546E-4 32-33 2.4970417858093737E-4 0.0 0.0 0.018899485016344697 3.1213022322617174E-4 34-35 3.1213022322617174E-4 0.0 0.0 0.03548920638081573 3.745562678714061E-4 36-37 3.433432455487889E-4 0.0 0.0 0.06102145864071658 3.745562678714061E-4 38-39 3.745562678714061E-4 0.0 0.0 0.09988167143237496 4.0576929019402327E-4 40-41 3.901627790327147E-4 0.0 0.0 0.1429088227041027 4.0576929019402327E-4 42-43 4.838018460005662E-4 0.0 0.0 0.187933607404478 4.0576929019402327E-4 44-45 4.994083571618747E-4 0.0 0.0 0.24380491736196275 4.0576929019402327E-4 46-47 5.930474241297263E-4 0.0 0.0 0.30765115452287617 4.0576929019402327E-4 48-49 6.554734687749606E-4 0.0 0.0 0.3656293434871376 4.0576929019402327E-4 50-51 6.554734687749606E-4 1.5606511161308586E-5 0.0 0.42164111204507415 4.369823125166404E-4 52-53 6.710799799362693E-4 3.121302232261717E-5 0.0 0.4792135317191415 4.838018460005662E-4 54-55 6.866864910975778E-4 3.121302232261717E-5 0.0 0.5441522246613466 5.462278906458006E-4 56-57 6.866864910975778E-4 3.121302232261717E-5 0.0 0.6001952062416056 5.618344018071092E-4 58-59 6.866864910975778E-4 3.121302232261717E-5 0.0 0.6357780516893892 5.618344018071092E-4 60-61 6.866864910975778E-4 4.681953348392576E-5 0.0 0.664041443402519 5.618344018071092E-4 62-63 8.739646250332809E-4 7.803255580654294E-5 0.0 0.6910563142227442 5.618344018071092E-4 64-65 9.363906696785152E-4 9.363906696785153E-5 0.0 0.7188515106010348 5.618344018071092E-4 66-67 9.676036920011324E-4 9.363906696785153E-5 0.0 0.7431664549903536 5.930474241297263E-4 68-69 9.676036920011324E-4 9.363906696785153E-5 0.0 0.7758308828509726 5.930474241297263E-4 70-71 9.676036920011324E-4 9.363906696785153E-5 0.0 0.827644499906517 5.930474241297263E-4 72-73 9.676036920011324E-4 9.363906696785153E-5 0.0 0.90269621208125 7.022930022588864E-4 74-75 9.676036920011324E-4 9.363906696785153E-5 0.0 0.9979739627210389 7.178995134201951E-4 76-77 9.676036920011324E-4 9.363906696785153E-5 0.0 1.1553968408051587 7.178995134201951E-4 78-79 9.676036920011324E-4 9.363906696785153E-5 0.0 1.401308637173898 7.178995134201951E-4 80-81 9.988167143237495E-4 9.363906696785153E-5 0.0 1.7001265063794735 7.178995134201951E-4 82-83 9.988167143237495E-4 9.363906696785153E-5 0.0 1.9943092417701402 7.803255580654294E-4 84-85 0.0010300297366463667 9.363906696785153E-5 0.0 2.2241619381538933 7.803255580654294E-4 86-87 0.0010300297366463667 9.363906696785153E-5 0.0 2.395755528372481 7.803255580654294E-4 88-89 0.0010300297366463667 1.2485208929046869E-4 0.0 2.5641966033364847 7.959320692267379E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9535 0.0 22.308329 1 TACATGG 9215 0.0 22.056807 2 ACATGGG 8820 0.0 21.601622 3 AGAGTAC 12225 0.0 20.117355 10-11 GTATCAA 18490 0.0 19.114033 1 AGTACTT 8650 0.0 18.845879 12-13 GAGTACT 8030 0.0 18.750616 12-13 GTACTTT 8535 0.0 17.531092 14-15 AACGCAG 20795 0.0 16.785448 6 TCAACGC 20800 0.0 16.73664 4 ATCAACG 20850 0.0 16.696507 3 CAACGCA 21025 0.0 16.579908 5 CATGGGG 5130 0.0 16.506502 4 GGTATCA 14675 0.0 16.290518 1 GAGTACA 7465 0.0 16.201403 1 TAGCCCT 2420 0.0 16.134764 4 GTATAGC 1535 0.0 15.942052 1 TATCAAC 21900 0.0 15.919459 2 CATGGGA 4540 0.0 15.335775 4 AGTACAT 6875 0.0 15.329555 2 >>END_MODULE