FastQCFastQC Report
Fri 27 May 2016
SRR939606_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939606_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2924168
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31530.10782554217131164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG76250.025.462431
TACATGG82400.023.2171732
ACATGGG81000.022.5687483
GTATCAA157050.021.800311
GTATAGG8500.020.8764531
GAGTACT66150.020.15380512-13
AGAGTAC107550.019.85513710-11
GTCTAGA12150.019.7596071
GTATAAT18000.019.716651
TATCAAC188050.018.7641452
GTACTTT70200.018.69017414-15
AGTACTT71600.018.5538412-13
CATGGGG44150.018.5070574
ATCAACG196900.017.968743
TCAACGC198400.017.7828314
CAACGCA197400.017.762295
GGTATCA132400.017.5810031
AACGCAG202150.017.4146586
TTTGCGT4650.017.2694113
GTATAGC12450.016.7682361
Produced by FastQC (version 0.11.5)