##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939606_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2924168 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.765250491763812 2.0 2.0 31.0 2.0 33.0 2 22.30400544701946 16.0 16.0 31.0 16.0 34.0 3 26.92614206844477 28.0 25.0 31.0 16.0 34.0 4 32.20434769821706 35.0 32.0 35.0 25.0 37.0 5 32.82884533309988 35.0 33.0 35.0 26.0 37.0 6 33.22750402849631 35.0 33.0 37.0 28.0 37.0 7 33.397296598553844 35.0 33.0 37.0 28.0 37.0 8 33.321732540674816 35.0 33.0 37.0 26.0 37.0 9 34.69589401156158 37.0 34.0 39.0 27.0 39.0 10-11 34.533373253520324 37.0 33.5 39.0 27.0 39.0 12-13 34.54210941368622 37.0 33.5 39.0 27.0 39.0 14-15 35.23750294100749 38.0 33.5 40.0 26.5 41.0 16-17 34.84321489052613 38.0 33.0 40.0 25.0 41.0 18-19 35.08098132528637 38.0 33.5 40.0 26.0 41.0 20-21 35.067808347536804 38.0 34.0 40.0 25.0 41.0 22-23 34.87513627807978 38.0 33.5 40.0 25.0 41.0 24-25 34.69257956451203 38.0 33.0 40.0 24.0 41.0 26-27 34.7947609371281 38.0 33.0 40.0 24.5 41.0 28-29 34.54724232670627 38.0 33.0 40.0 24.0 41.0 30-31 34.43024768070781 38.0 33.0 40.0 24.0 41.0 32-33 34.136555423628195 38.0 33.0 40.0 23.0 41.0 34-35 33.86898752055286 38.0 32.0 40.0 21.0 41.0 36-37 33.61541676128047 38.0 32.0 40.0 19.0 41.0 38-39 33.417967435523536 38.0 31.0 40.0 18.5 41.0 40-41 33.0609414370173 37.0 31.0 40.0 17.0 41.0 42-43 32.816262437725875 37.0 31.0 40.0 15.5 41.0 44-45 32.483503512794066 37.0 30.0 40.0 15.0 41.0 46-47 32.18342242990143 37.0 30.0 39.5 11.5 41.0 48-49 31.8311694129749 36.5 30.0 39.0 9.0 40.0 50-51 31.171862902541854 35.5 29.5 38.5 9.0 40.0 52-53 31.23077692526558 36.0 30.0 39.0 8.0 40.0 54-55 31.350911267752057 36.0 29.5 39.0 7.5 40.0 56-57 31.042109755663834 36.0 29.0 39.0 6.5 40.0 58-59 30.624666913802493 35.0 28.0 39.0 2.0 40.0 60-61 30.03574213246298 35.0 28.0 38.0 2.0 40.0 62-63 29.63861173503027 34.0 27.0 38.0 2.0 40.0 64-65 29.070043855209413 34.0 26.0 38.0 2.0 40.0 66-67 28.45231447030403 34.0 26.0 37.0 2.0 39.0 68-69 28.085884942315214 33.0 25.5 37.0 2.0 39.0 70-71 27.552879827698 33.0 24.5 36.0 2.0 39.0 72-73 26.948766965509506 32.0 23.0 36.0 2.0 38.5 74-75 26.382182555858623 32.0 22.0 35.0 2.0 38.0 76-77 25.740755832086258 31.0 20.5 35.0 2.0 37.0 78-79 25.05735614369626 31.0 18.5 34.5 2.0 36.5 80-81 24.3044537796734 30.5 15.0 34.0 2.0 36.0 82-83 23.59784082173117 30.0 7.5 34.0 2.0 35.5 84-85 22.877648103665727 29.0 4.5 34.0 2.0 35.0 86-87 22.197997173896987 29.0 2.0 34.0 2.0 35.0 88-89 21.39192891790075 29.0 2.0 33.0 2.0 35.0 90-91 20.47075475827654 26.5 2.0 33.0 2.0 34.0 92-93 19.437862154294827 25.5 2.0 32.0 2.0 34.0 94-95 18.43055204078562 25.0 2.0 32.0 2.0 34.0 96-97 17.42524933587947 22.0 2.0 31.5 2.0 34.0 98-99 16.314986177264778 14.5 2.0 31.0 2.0 34.0 100-101 14.733473589752709 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.981928641371653 1101 2 8.113936363657796 1101 3 3.9836603260074774 1101 4 2.6357328093149945 1101 5 1.8521192317481336 1101 6 1.594788566269962 1101 7 1.2159412495001405 1101 8 1.1248927194717169 1101 9 1.3820035342235002 1101 10-11 1.4286090423203248 1101 12-13 0.9480401722890406 1101 14-15 1.127838652074857 1101 16-17 1.2062640250471546 1101 18-19 1.2667548637102684 1101 20-21 1.3240740717582113 1101 22-23 1.175257368945779 1101 24-25 1.441485012549414 1101 26-27 1.4136440338792085 1101 28-29 1.567310375684201 1101 30-31 1.5551170679184096 1101 32-33 1.4424577343848668 1101 34-35 1.543622423718908 1101 36-37 1.5265635791891228 1101 38-39 1.3598438306732419 1101 40-41 1.5046411407403397 1101 42-43 1.469154159183205 1101 44-45 1.2048014674105545 1101 46-47 1.1777252003558587 1101 48-49 0.9903032227631385 1101 50-51 1.0623865528642504 1101 52-53 1.3212939691513625 1101 54-55 1.3659737893548645 1101 56-57 0.9905367688414195 1101 58-59 1.1044989689107574 1101 60-61 1.0490368029914876 1101 62-63 0.7410394858219256 1101 64-65 0.8278037735198929 1101 66-67 0.6154616409579532 1101 68-69 0.6563842243970512 1101 70-71 0.5600463326585832 1101 72-73 0.4634504929531147 1101 74-75 0.4483867761906879 1101 76-77 0.4728482348347782 1101 78-79 0.4279057759389673 1101 80-81 0.26965083806344126 1101 82-83 0.37318179674304375 1101 84-85 0.21750901127753508 1101 86-87 0.06281008484285877 1101 88-89 0.13459787458580763 1101 90-91 0.3973526628211701 1101 92-93 0.2679197359650587 1101 94-95 0.2147180561188229 1101 96-97 -0.30508715898352534 1101 98-99 -0.5576508917142515 1101 100-101 -0.6990491059063757 1102 1 14.872729368971424 1102 2 8.044578752725482 1102 3 3.8008328666984887 1102 4 2.3017825701724988 1102 5 1.7195706281286078 1102 6 1.4356997813810963 1102 7 1.179139355278373 1102 8 1.1310108223071182 1102 9 1.248812969447826 1102 10-11 1.17913370316289 1102 12-13 0.9611664527392634 1102 14-15 1.0332466000438174 1102 16-17 1.3732297605933184 1102 18-19 1.2176819960047496 1102 20-21 1.231707722797104 1102 22-23 1.0342916701849987 1102 24-25 0.9829143103015312 1102 26-27 1.1025523883641384 1102 28-29 1.1428339948999806 1102 30-31 1.1294407049263029 1102 32-33 1.174303164852617 1102 34-35 1.259837305881824 1102 36-37 1.3095739988352122 1102 38-39 1.1851808954703102 1102 40-41 1.1151937303183672 1102 42-43 1.1314456179937196 1102 44-45 1.06310085872515 1102 46-47 0.9875594803496384 1102 48-49 0.9626239146885922 1102 50-51 0.8836006054793479 1102 52-53 1.1262713123611974 1102 54-55 1.0744613491404884 1102 56-57 0.9899850820066085 1102 58-59 0.8513715548703047 1102 60-61 1.056447320681876 1102 62-63 0.7702922603027851 1102 64-65 0.677992880393429 1102 66-67 0.573738904741397 1102 68-69 0.3100257234838004 1102 70-71 0.6013281604564646 1102 72-73 0.5924244868167108 1102 74-75 0.5060283379627215 1102 76-77 0.24519788586165348 1102 78-79 0.36359179696969335 1102 80-81 0.37657392133878176 1102 82-83 0.23079146221381208 1102 84-85 0.02179465799827085 1102 86-87 -0.0871704714571635 1102 88-89 -0.003754232428111237 1102 90-91 0.19223317039933718 1102 92-93 0.3281556053124035 1102 94-95 0.14896747674911026 1102 96-97 -0.7731656616084237 1102 98-99 -0.8700754441525582 1102 100-101 -0.9257500261414116 1103 1 14.836910923761632 1103 2 7.879791246517492 1103 3 3.838549446342764 1103 4 2.3365992631448336 1103 5 1.6108147461961053 1103 6 1.2654065063476096 1103 7 0.9181701866995695 1103 8 0.9977879064601964 1103 9 1.1771582103325784 1103 10-11 1.0105112432808738 1103 12-13 0.7955743025862176 1103 14-15 1.0619589228858288 1103 16-17 1.0678436199221082 1103 18-19 0.9594656197320219 1103 20-21 0.8193591093341936 1103 22-23 0.8911309963472718 1103 24-25 1.0461951907234734 1103 26-27 1.122076517366537 1103 28-29 1.178510687931265 1103 30-31 1.1836880386221722 1103 32-33 0.9919620599261663 1103 34-35 1.0840929733899785 1103 36-37 1.1342309751424295 1103 38-39 1.1605107663293808 1103 40-41 1.0585498729496123 1103 42-43 1.1147717301634401 1103 44-45 0.9753299177191082 1103 46-47 0.8302977345375453 1103 48-49 0.888810359238164 1103 50-51 0.8049588179318796 1103 52-53 0.9030638556992301 1103 54-55 0.9230406404643325 1103 56-57 0.9581473032365331 1103 58-59 0.7327722321859795 1103 60-61 0.8876311604545677 1103 62-63 0.8940168591229316 1103 64-65 0.7130682089195268 1103 66-67 0.697463871988262 1103 68-69 0.8106399829239983 1103 70-71 0.49843836867650637 1103 72-73 0.5710162235949845 1103 74-75 0.3986654774387617 1103 76-77 0.4631740516006211 1103 78-79 0.41526143315567765 1103 80-81 0.4339571190173963 1103 82-83 0.20802757289509088 1103 84-85 0.237722186989771 1103 86-87 -0.038297562462986434 1103 88-89 -0.12295399855083389 1103 90-91 -0.040115321482751654 1103 92-93 0.19156619035928912 1103 94-95 0.16113053209712191 1103 96-97 -0.5178020133881454 1103 98-99 -0.8706228341440259 1103 100-101 -0.6418204125915601 1104 1 14.670893828108081 1104 2 7.9427060499248405 1104 3 3.800812899090385 1104 4 2.3655239822521636 1104 5 1.6896916909969875 1104 6 1.2680789218864987 1104 7 0.932864053730512 1104 8 0.9413494211726601 1104 9 1.0059730209198818 1104 10-11 0.7902369874646453 1104 12-13 0.6272634879192367 1104 14-15 0.8639156443349307 1104 16-17 1.066395846720276 1104 18-19 0.9341982585122466 1104 20-21 0.9208707657126709 1104 22-23 0.9328059322339612 1104 24-25 1.0701680431349345 1104 26-27 0.955785939122535 1104 28-29 1.0809931108526172 1104 30-31 0.9298369140389227 1104 32-33 0.9520456603599072 1104 34-35 0.9544179491346227 1104 36-37 1.0722104441572498 1104 38-39 0.7823601934979578 1104 40-41 1.1105025441684901 1104 42-43 0.8838964570030328 1104 44-45 0.6486275783429605 1104 46-47 0.5856151345106682 1104 48-49 0.6034735733904029 1104 50-51 0.5958596075426712 1104 52-53 0.6684323622136574 1104 54-55 0.7336019839625258 1104 56-57 0.6571093396922905 1104 58-59 0.5981750736065798 1104 60-61 0.3946613475567986 1104 62-63 0.40542203126691945 1104 64-65 0.43115478811349917 1104 66-67 0.4791683377352811 1104 68-69 0.5409214371039255 1104 70-71 0.49877748340664496 1104 72-73 0.49374059254149927 1104 74-75 0.29619281189020086 1104 76-77 0.08941227141398045 1104 78-79 -0.034253919978368685 1104 80-81 0.10123650573889975 1104 82-83 0.02377164621162109 1104 84-85 0.21868969493834456 1104 86-87 -0.025039409993908635 1104 88-89 -0.043250438582571604 1104 90-91 0.025832183851587587 1104 92-93 0.18049958064131744 1104 94-95 0.09415472854064788 1104 96-97 -0.6901777863837886 1104 98-99 -0.8087557516537238 1104 100-101 -0.829591294750113 1105 1 14.679057200331101 1105 2 7.870723181218921 1105 3 3.8661702080028313 1105 4 2.327688913056221 1105 5 1.5888790958660124 1105 6 1.1899984089206228 1105 7 1.0277980104962623 1105 8 0.9879597880753579 1105 9 1.0179409773918735 1105 10-11 0.796606401107006 1105 12-13 0.7852029895287984 1105 14-15 0.8738675491061869 1105 16-17 1.0438760346855176 1105 18-19 0.9911197241771461 1105 20-21 0.946156778430165 1105 22-23 0.8186307031945859 1105 24-25 1.0307711185726802 1105 26-27 0.9279760729116546 1105 28-29 1.0566234427480552 1105 30-31 0.8977790257571954 1105 32-33 0.5718655535044874 1105 34-35 0.9214035823105107 1105 36-37 0.8702261534566276 1105 38-39 0.9631009124098284 1105 40-41 0.9732925445520522 1105 42-43 0.9091515039148277 1105 44-45 0.7717137558995759 1105 46-47 0.7638720403063317 1105 48-49 0.9033103246564025 1105 50-51 0.9172336379901971 1105 52-53 0.9892777287093963 1105 54-55 0.8642682352570539 1105 56-57 0.8556801414634201 1105 58-59 1.0152128154041478 1105 60-61 0.9035938598752935 1105 62-63 0.6701074476821667 1105 64-65 0.7439047948960358 1105 66-67 0.8837342036879576 1105 68-69 0.6329360013233583 1105 70-71 0.5901366203306999 1105 72-73 0.5611272375788801 1105 74-75 0.4379299000379717 1105 76-77 0.495175138828877 1105 78-79 0.5677444503408609 1105 80-81 0.4150540376238574 1105 82-83 0.5082941693915366 1105 84-85 0.44036926714246505 1105 86-87 0.33226066176069224 1105 88-89 0.4779721774947632 1105 90-91 0.7637798899963819 1105 92-93 1.0130193365446978 1105 94-95 0.9392912259993018 1105 96-97 0.3912427301932091 1105 98-99 -0.014724527643718233 1105 100-101 -0.06973691823397843 1106 1 14.762580650014783 1106 2 8.044762589959216 1106 3 3.661517565792799 1106 4 2.3660928444994482 1106 5 1.7301241843483481 1106 6 1.193660131276161 1106 7 1.124513434397855 1106 8 1.0821408988692554 1106 9 1.1560824753972625 1106 10-11 1.0546832381196367 1106 12-13 0.7861932235069418 1106 14-15 0.9736882781756009 1106 16-17 1.2289344756371392 1106 18-19 1.1543432915743637 1106 20-21 0.921626410826164 1106 22-23 1.0365541954026014 1106 24-25 1.0040853845435365 1106 26-27 1.0143617703321581 1106 28-29 1.2007140607312152 1106 30-31 1.1026995884158524 1106 32-33 1.0140508927260683 1106 34-35 1.1946550048609268 1106 36-37 1.2920542539820943 1106 38-39 1.2870017312604602 1106 40-41 1.3554812788615607 1106 42-43 1.0518099263477865 1106 44-45 0.9719022004263209 1106 46-47 1.0992312809040357 1106 48-49 0.9722901950429161 1106 50-51 1.0796032893335301 1106 52-53 0.8924487065375928 1106 54-55 0.9792674079187087 1106 56-57 1.0813171705557245 1106 58-59 0.9875424201421801 1106 60-61 1.0260733714627435 1106 62-63 0.9892049887297212 1106 64-65 1.049557016149457 1106 66-67 1.1232310790925162 1106 68-69 1.0846507350618744 1106 70-71 1.2409642587469776 1106 72-73 1.131373835846528 1106 74-75 1.002307540126072 1106 76-77 0.8638881175812863 1106 78-79 0.8230482035419726 1106 80-81 0.8432290664092328 1106 82-83 0.8507119349607173 1106 84-85 0.7862288459629454 1106 86-87 0.839796728139806 1106 88-89 0.6898736753629748 1106 90-91 1.0621150966294906 1106 92-93 1.453416253187573 1106 94-95 1.063163495246922 1106 96-97 0.7032787455602652 1106 98-99 0.6072873020086007 1106 100-101 0.4185478850609705 1107 1 14.631381076695092 1107 2 7.878338025127004 1107 3 3.668626200135641 1107 4 2.2943207109535706 1107 5 1.4969421493326749 1107 6 1.2199589088057508 1107 7 0.8729400554684403 1107 8 0.9056072971816249 1107 9 0.8339400670702801 1107 10-11 0.7111805429267406 1107 12-13 0.5866290068089626 1107 14-15 0.7158421364619301 1107 16-17 0.8889848713059578 1107 18-19 0.6624595751654638 1107 20-21 0.7782750988492992 1107 22-23 0.8089360689981788 1107 24-25 0.9934364884250684 1107 26-27 0.7487016303687142 1107 28-29 0.8520987860628395 1107 30-31 0.9857917113475523 1107 32-33 1.016285842156364 1107 34-35 0.6602194738013694 1107 36-37 0.7419852551951038 1107 38-39 0.7366836029411132 1107 40-41 0.8945350922555235 1107 42-43 0.6827344289683595 1107 44-45 0.6948929415050813 1107 46-47 0.5556451683520081 1107 48-49 0.6906385992508177 1107 50-51 0.7565319042421912 1107 52-53 0.6426582404163312 1107 54-55 0.6809062104706776 1107 56-57 0.5994225013145602 1107 58-59 0.6374299156602561 1107 60-61 0.70745782983618 1107 62-63 0.6841083795798113 1107 64-65 0.3854267824365252 1107 66-67 0.45578098918986143 1107 68-69 0.4220299470937441 1107 70-71 0.4708408231290413 1107 72-73 0.561365371479642 1107 74-75 0.42709084412702225 1107 76-77 0.5119218573230846 1107 78-79 0.6739220105195045 1107 80-81 0.5135017910835558 1107 82-83 0.656906421453435 1107 84-85 0.6528533747416532 1107 86-87 0.7242622707709998 1107 88-89 0.8205520198939311 1107 90-91 1.093210726052284 1107 92-93 1.494571065259855 1107 94-95 1.4404697003457123 1107 96-97 0.9428186775529035 1107 98-99 0.8190143884825858 1107 100-101 0.7898800281192422 1108 1 14.240607968862378 1108 2 7.4344809642265375 1108 3 3.3054364723727723 1108 4 -0.09626958171003963 1108 5 0.08614491027034887 1108 6 0.4297436015481466 1108 7 0.5061307364418965 1108 8 0.6254715629751288 1108 9 0.5268683533010616 1108 10-11 0.5450758170428074 1108 12-13 0.21058449345837715 1108 14-15 0.34541112966748955 1108 16-17 0.5863481957690624 1108 18-19 0.36660364656363953 1108 20-21 0.21598027090660565 1108 22-23 -0.47287780037432015 1108 24-25 0.15310671669525533 1108 26-27 0.21240373444831562 1108 28-29 0.2206023600434932 1108 30-31 0.34563453316398096 1108 32-33 0.5066728111805574 1108 34-35 0.4873831057703981 1108 36-37 0.31488485379374964 1108 38-39 0.32944287365953073 1108 40-41 0.45536157522245446 1108 42-43 0.3815105177505629 1108 44-45 0.3027814719590438 1108 46-47 0.19311546671065116 1108 48-49 0.20800431652408946 1108 50-51 0.20656820171880952 1108 52-53 0.3978140335094409 1108 54-55 0.3640920881199925 1108 56-57 0.3702572109641018 1108 58-59 0.13527237985965357 1108 60-61 0.23218707563229657 1108 62-63 0.22866766690254892 1108 64-65 0.0314252407139044 1108 66-67 0.1187300103134028 1108 68-69 0.11001085604666727 1108 70-71 0.01200675528933104 1108 72-73 -0.1335170896411526 1108 74-75 -0.0972121124750629 1108 76-77 -0.1628440866148999 1108 78-79 -0.014501913072329842 1108 80-81 0.061245528569170204 1108 82-83 0.16645613800140424 1108 84-85 0.1254572357942969 1108 86-87 -0.13081007213584073 1108 88-89 -0.03837318531423506 1108 90-91 0.3060832434069134 1108 92-93 0.7411640828198927 1108 94-95 0.41695540204707626 1108 96-97 -0.14858783545646403 1108 98-99 -0.20080158317118446 1108 100-101 -0.23416608341981515 1109 1 14.187929676435044 1109 2 6.450153512295213 1109 3 2.898594633238492 1109 4 -3.6650450812151973 1109 5 -0.748121764689067 1109 6 0.031899741901334266 1109 7 -0.0015067370474497466 1109 8 0.07500657075521389 1109 9 0.38895996427508095 1109 10-11 0.3343252547923967 1109 12-13 0.18095527338599027 1109 14-15 0.34461118295655524 1109 16-17 0.43829454329759443 1109 18-19 0.25156197079154197 1109 20-21 0.4837393791377167 1109 22-23 0.35746232894373975 1109 24-25 0.5185316190377662 1109 26-27 0.4331140067054733 1109 28-29 0.6541310584429567 1109 30-31 0.4848002698712648 1109 32-33 0.4652872540589428 1109 34-35 0.3761878353443464 1109 36-37 0.35251917804193766 1109 38-39 0.5455871220572419 1109 40-41 0.5948735217635175 1109 42-43 0.6452515719609124 1109 44-45 0.5059375433521183 1109 46-47 0.30078851008956775 1109 48-49 0.22807523269959518 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2312 62-63 0.3577612358134239 2312 64-65 0.46423674028416784 2312 66-67 0.5013521626385717 2312 68-69 0.6747101733285277 2312 70-71 0.4036384853176678 2312 72-73 0.19457604255786976 2312 74-75 0.45805477763971325 2312 76-77 0.41928025699155214 2312 78-79 0.6400410996961448 2312 80-81 0.7663670124293454 2312 82-83 0.6456104502379141 2312 84-85 0.7012576307515133 2312 86-87 0.7170257573273418 2312 88-89 1.1841467165214254 2312 90-91 1.2801696420189614 2312 92-93 1.8758696409690927 2312 94-95 1.9463910743163169 2312 96-97 1.5548836051325843 2312 98-99 1.2772946750436773 2312 100-101 1.3104452793128285 2313 1 -14.146735351223224 2313 2 -7.399598167040532 2313 3 -3.74805417860248 2313 4 -1.2449083381885409 2313 5 -0.6838015766613026 2313 6 -1.2505071516988906 2313 7 -0.45381418407590957 2313 8 -0.32258066165478283 2313 9 -2.85000287842049 2313 10-11 -0.7058261318954635 2313 12-13 -0.9007933221051303 2313 14-15 -1.2414276034035439 2313 16-17 -0.9302756397068563 2313 18-19 -0.284468562312469 2313 20-21 -1.1969809676830678 2313 22-23 -2.213596938448795 2313 24-25 -1.479883079757819 2313 26-27 -0.5386140842797715 2313 28-29 -0.01621769655476868 2313 30-31 -1.361859771656043 2313 32-33 -0.40102408129535405 2313 34-35 -1.554561524629257 2313 36-37 -1.3919396853447878 2313 38-39 -0.9026282028590842 2313 40-41 -0.4731793163519171 2313 42-43 -0.4180424518366266 2313 44-45 -0.46305917994559564 2313 46-47 -1.0724842918542592 2313 48-49 -0.5367544319252602 2313 50-51 -1.343481849733731 2313 52-53 -0.3329630002295474 2313 54-55 -1.187697271156967 2313 56-57 -0.7947051283185225 2313 58-59 -0.5422663384439375 2313 60-61 -2.0003602299257324 2313 62-63 -0.7243845189639906 2313 64-65 -0.6086004813519743 2313 66-67 -1.758516292057898 2313 68-69 -0.6292048703373574 2313 70-71 -0.2821310646567703 2313 72-73 -1.1977450545090207 2313 74-75 -0.6786824454655047 2313 76-77 -0.22172541493383946 2313 78-79 -0.22322669808272622 2313 80-81 -0.17790187153411452 2313 82-83 -0.03441967134744672 2313 84-85 0.16993549622119986 2313 86-87 0.13133428210047526 2313 88-89 0.5094809911775435 2313 90-91 1.0247856736274734 2313 92-93 1.361966301541898 2313 94-95 1.3755958183451362 2313 96-97 1.0737711483005619 2313 98-99 0.7919888966941393 2313 100-101 0.7253447807390394 2314 1 -14.146735351223224 2314 2 -7.209916920356518 2314 3 -3.656998349559924 2314 4 -1.4678971006287576 2314 5 -1.4374805814022302 2314 6 -6.270855872724912 2314 7 -2.196366532859823 2314 8 -4.481336883302568 2314 9 -1.8788230037806457 2314 10-11 -0.6238495472824752 2314 12-13 -3.261318549742093 2314 14-15 -3.1835654976780106 2314 16-17 -3.0748511830172163 2314 18-19 -1.6801771525193274 2314 20-21 -2.2942497313128314 2314 22-23 -1.5383895407185122 2314 24-25 -3.337763994620616 2314 26-27 -5.008665789518428 2314 28-29 -2.4150854830943587 2314 30-31 -2.3356582930267606 2314 32-33 -4.635354961864159 2314 34-35 -3.1678687714129055 2314 36-37 -1.3870444321611117 2314 38-39 -1.9465043512251015 2314 40-41 -3.031131726772216 2314 42-43 -2.158839896748873 2314 44-45 -3.1250175956517694 2314 46-47 -2.7302632623295935 2314 48-49 -1.633239907691305 2314 50-51 -2.6896466420215646 2314 52-53 -1.5057824614926645 2314 54-55 -0.8709054659552287 2314 56-57 -1.6092099091163057 2314 58-59 -1.9919300185681337 2314 60-61 -1.0349812599049955 2314 62-63 -3.4077565123669658 2314 64-65 -1.5366639603907721 2314 66-67 -2.9609008568972364 2314 68-69 -2.9091478140601517 2314 70-71 -3.444814569763178 2314 72-73 -3.4963013727217387 2314 74-75 -2.7952347419702406 2314 76-77 -1.4720758842814803 2314 78-79 -1.0978822664113217 2314 80-81 -0.8684158439954928 2314 82-83 -0.9601722594032829 2314 84-85 -0.7225713734704975 2314 86-87 -0.5822873216237703 2314 88-89 -0.2433022591815437 2314 90-91 0.2479481790803213 2314 92-93 0.6703558397216973 2314 94-95 0.5086550743253717 2314 96-97 0.38975021124942444 2314 98-99 0.46284599479468014 2314 100-101 0.3702112216581739 2315 1 -14.146735351223224 2315 2 -7.355941332791042 2315 3 -4.672622020945468 2315 4 -2.9796414387305887 2315 5 -3.4365074342161464 2315 6 -2.9211399028798084 2315 7 -3.2353059703294953 2315 8 -3.404756159805782 2315 9 -2.325373059051003 2315 10-11 -4.659701346127029 2315 12-13 -4.672134337918656 2315 14-15 -3.607163821088079 2315 16-17 -4.594247505775705 2315 18-19 -1.5824991851961059 2315 20-21 -3.383018261710351 2315 22-23 -4.552604402112543 2315 24-25 -5.339066417267148 2315 26-27 -4.858506851636264 2315 28-29 -2.8442102535048726 2315 30-31 -3.4445634391107802 2315 32-33 -4.625306929966214 2315 34-35 -3.9267023576473328 2315 36-37 -5.0587632785393595 2315 38-39 -3.6525298515892857 2315 40-41 -4.717265199245336 2315 42-43 -3.391564693159726 2315 44-45 -2.144162506507328 2315 46-47 -1.6027171092704613 2315 48-49 -1.3354113092412305 2315 50-51 -1.0202649788206308 2315 52-53 -0.8392799825251558 2315 54-55 -2.5458304155010154 2315 56-57 -4.573506178676212 2315 58-59 -3.8757188062551258 2315 60-61 -4.733838305359047 2315 62-63 -2.5632649123032607 2315 64-65 -3.4820879888293312 2315 66-67 -2.1319993477188603 2315 68-69 -1.6114572687776345 2315 70-71 -2.1824354914752497 2315 72-73 -2.4588470377835456 2315 74-75 -2.8505580760386806 2315 76-77 -2.6435347763488437 2315 78-79 -1.9695527156300443 2315 80-81 -1.4469653961474833 2315 82-83 -0.9035179298065508 2315 84-85 -0.8832246595188238 2315 86-87 -1.6806716086204005 2315 88-89 -2.235466318188177 2315 90-91 -1.6387517532284583 2315 92-93 -0.7697585421388631 2315 94-95 -0.5659127338361039 2315 96-97 -1.5977529823346988 2315 98-99 -1.0344996237650061 2315 100-101 -0.6755048000305095 2316 1 -14.146735351223224 2316 2 -7.383687605226536 2316 3 -5.099723200210299 2316 4 -3.0966085698597965 2316 5 -2.1411212922525067 2316 6 -1.4635336049902676 2316 7 -4.840929475244895 2316 8 -3.1352100751534806 2316 9 -1.580836539534154 2316 10-11 -4.570952216071841 2316 12-13 -3.8239548925716242 2316 14-15 -2.056172282685047 2316 16-17 -3.27145852799066 2316 18-19 -2.568752070347493 2316 20-21 -3.3578758632398618 2316 22-23 -2.9377694163098127 2316 24-25 -3.653887097941489 2316 26-27 -4.230016144860745 2316 28-29 -3.881119187738747 2316 30-31 -2.6594111405831846 2316 32-33 -2.9915074310998406 2316 34-35 -4.231495256854082 2316 36-37 -2.5283512567819386 2316 38-39 -3.265724288937932 2316 40-41 -2.8764897931670177 2316 42-43 -6.820469340396393 2316 44-45 -3.66683670266697 2316 46-47 -4.858168668813285 2316 48-49 -4.600847507800957 2316 50-51 -3.431686715576749 2316 52-53 -4.894738467566647 2316 54-55 -5.072967584652346 2316 56-57 -4.898203074576884 2316 58-59 -3.8624445553726936 2316 60-61 -3.584085182356514 2316 62-63 -2.64773499244604 2316 64-65 -2.732105870239817 2316 66-67 -3.121758124653091 2316 68-69 -3.133143162218051 2316 70-71 -3.1735515526149136 2316 72-73 -3.974334609919424 2316 74-75 -4.53012433546332 2316 76-77 -4.1232294413306825 2316 78-79 -4.325424573468858 2316 80-81 -4.383468223680996 2316 82-83 -3.7603542484839387 2316 84-85 -3.3889813383675538 2316 86-87 -3.6566639701359698 2316 88-89 -3.272748775218865 2316 90-91 -2.54504691798153 2316 92-93 -2.644287806912029 2316 94-95 -1.855491107206765 2316 96-97 -1.9693360718564197 2316 98-99 -2.190937555970052 2316 100-101 -2.056872143238923 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22027.0 3 6563.0 4 7341.0 5 8685.0 6 9828.0 7 11917.0 8 14465.0 9 17911.0 10 21056.0 11 24625.0 12 27785.0 13 30429.0 14 33515.0 15 36489.0 16 40313.0 17 42154.0 18 41498.0 19 42914.0 20 46206.0 21 50400.0 22 55757.0 23 61983.0 24 68931.0 25 76992.0 26 85729.0 27 95387.0 28 108775.0 29 123121.0 30 139238.0 31 157743.0 32 181841.0 33 209267.0 34 244562.0 35 284337.0 36 296624.0 37 175219.0 38 22435.0 39 106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.375481199684174 19.165253132756334 13.825154985020948 23.63411068253854 2 15.131070272312785 20.773993839839243 40.54975960723927 23.545176280608704 3 19.147595808508292 25.285181256749066 30.123042727715326 25.444180207027316 4 12.404605130165987 16.931617202152495 39.01320401362544 31.650573654056075 5 13.445917907575836 37.66247772800293 35.114225728830135 13.777378635591099 6 30.73069243862094 37.26041705907937 18.329880101507893 13.679010400791789 7 27.152868824946946 32.80992913048184 22.409967764164655 17.627234280406558 8 25.291626367544435 35.105478671375614 20.77499664364251 18.82789831743744 9 26.131462399077314 16.747968704679607 20.91449125927468 36.206077636968395 10-11 24.449745226343065 26.43207140065334 28.71747576256187 20.400707610441724 12-13 25.694038981723445 24.084074695165278 28.368574773756084 21.853311549355194 14-15 22.772754140961528 25.38781167229571 26.739707271366115 25.099726915376642 16-17 22.107387958257632 28.26244743207585 27.273183351337543 22.356981258328982 18-19 21.929749757956778 27.7701919872819 28.82115788273224 21.478900372029084 20-21 23.127744739700315 26.751987294742413 28.499970795858253 21.620297169699015 22-23 22.448280339933483 26.706665693587716 28.507769727034223 22.33728423944458 24-25 22.57144898824048 26.760565069787184 28.66034207364648 22.00764386832586 26-27 22.368853258177335 26.754224911793766 28.89476920904744 21.982152620981456 28-29 22.18617754349879 27.32662813206449 28.654822756879877 21.83237156755684 30-31 22.49607765346422 26.90536926611204 28.441061120055938 22.157491960367807 32-33 22.321705806269417 26.94844073333548 28.619690689638865 22.110162770756236 34-35 22.66804099406636 26.93232237115338 28.492284485771734 21.907352149008524 36-37 22.609421564509265 26.622693381154267 28.79298282160412 21.97490223273235 38-39 22.24453178693275 27.14803935071458 28.664016242413776 21.943412619938893 40-41 22.574027231455034 27.0572370361767 28.48380320800944 21.884932524358828 42-43 22.360242382716315 27.069969809219963 28.107727554975053 22.462060253088666 44-45 22.586460657400636 26.88865669273991 28.21347677641618 22.311405873443277 46-47 22.823335020730305 27.145955953797994 27.84126175708407 22.18944726838763 48-49 22.597408072392867 27.129074210949245 28.05813131900815 22.21538639764974 50-51 22.2457462266282 27.35760969454824 28.149458189981253 22.247185888842306 52-53 22.58519490890376 27.531494476176693 27.570410938989102 22.312899675930442 54-55 22.494803535412924 27.11603157750026 27.75909728419373 22.630067602893085 56-57 22.58888211585184 27.309845080630087 27.617425125903374 22.483847677614712 58-59 22.77610789267809 27.187345348626273 27.70057592621238 22.33597083248326 60-61 22.567239404745283 27.271389450186863 28.05581133992272 22.105559805145134 62-63 22.361231798644766 27.260161509616015 28.070433625359094 22.308173066380125 64-65 22.2865540612997 27.458787100346132 27.77915771751952 22.475501120834643 66-67 22.41292025831608 27.413044465212216 27.43528178261737 22.73875349385433 68-69 22.35492220434998 27.262600360071467 27.746915324963638 22.635562110614913 70-71 22.618775744602917 26.9737050624278 27.62636281603446 22.78115637693482 72-73 22.50787896484316 26.9402336478065 27.80243024425819 22.749457143092148 74-75 22.186193915556636 27.68935444386032 27.339696842282805 22.784754798300238 76-77 22.33122123113515 27.218487219227704 27.491747511740662 22.958544037896484 78-79 22.41858757494036 27.326969137374697 27.431694532305983 22.82274875537896 80-81 22.618787647956403 27.176326563914664 27.29139376844152 22.91349201968741 82-83 22.549435150498844 27.13240048997671 27.583334004620397 22.73483035490404 84-85 22.46719683577694 27.244835768715227 27.304583027700485 22.983384367807346 86-87 22.24895079682207 27.22730870538327 27.494683940127 23.029056557667662 88-89 22.382240223842796 27.179684292276068 27.186401769575447 23.25167371430569 90-91 22.36484717474086 27.072235944983454 27.332250666871367 23.23066621340432 92-93 22.52096149417725 27.20100829001908 27.339038204267823 22.93899201153585 94-95 22.43829318962503 27.093214993383658 27.269782174959378 23.198709642031936 96-97 22.155674146466918 26.975974624402582 27.54675410960372 23.32159711952678 98-99 22.267334432557618 27.153377799613644 27.03891099627275 23.540376771555984 100-101 22.392080995369742 26.944846751566708 27.10593345750648 23.557138795557066 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2922.0 1 1889.0 2 763.5 3 583.5 4 477.5 5 459.5 6 579.0 7 1104.0 8 1513.5 9 1474.0 10 1374.5 11 1387.5 12 1502.0 13 1647.5 14 1867.5 15 2080.5 16 2342.0 17 2638.0 18 2972.5 19 3338.5 20 3934.0 21 4790.5 22 5893.0 23 7448.0 24 9523.0 25 12701.5 26 17337.5 27 22316.0 28 26755.5 29 31647.0 30 36896.5 31 42455.0 32 48813.5 33 55838.5 34 62712.0 35 69576.5 36 77050.0 37 82569.5 38 86350.5 39 89763.0 40 91867.0 41 93503.5 42 95583.5 43 98813.5 44 102530.0 45 105495.0 46 109175.0 47 118313.5 48 124426.0 49 118360.5 50 109366.0 51 103650.5 52 100402.5 53 96415.5 54 91213.5 55 85145.5 56 77640.0 57 70716.5 58 63534.0 59 54898.0 60 45795.0 61 36944.0 62 28797.0 63 21621.5 64 15790.0 65 11356.5 66 8143.5 67 5881.5 68 4192.0 69 3013.0 70 2068.0 71 1306.5 72 827.0 73 519.0 74 319.0 75 208.5 76 147.0 77 101.0 78 59.0 79 38.5 80 29.0 81 22.0 82 18.5 83 16.5 84 11.5 85 9.0 86 9.5 87 10.5 88 7.0 89 3.0 90 2.5 91 3.0 92 3.5 93 2.0 94 2.0 95 5.5 96 5.5 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 54.738749620404846 2 0.08518662402433787 3 0.11606720270517974 4 0.18842966614777262 5 0.11832425496756684 6 0.1435622029924409 7 0.15170127024165506 8 0.14828149408652308 9 0.13634647530511243 10-11 0.12429176435827217 12-13 0.13607289321270186 14-15 0.14342541194623565 16-17 0.13738950703242767 18-19 0.14482752016983977 20-21 0.17309197009200566 22-23 0.19140487140273746 24-25 0.21901956385542826 26-27 0.2102649368982904 28-29 0.2114960563141379 30-31 0.2253803475039738 32-33 0.21619824852744438 34-35 0.20149321106037685 36-37 0.18815608405536205 38-39 0.18432593476161424 40-41 0.18504408775419195 42-43 0.1788713917941787 44-45 0.19350803373814365 46-47 0.22088334185997519 48-49 0.2233968773339972 50-51 0.23312614049534772 52-53 0.2621087434100913 54-55 0.24899390185516018 56-57 0.22411503032657493 58-59 0.18140202614897638 60-61 0.1910115971448973 62-63 0.19506403188872867 64-65 0.18333419967662595 66-67 0.19323445164573308 68-69 0.1813507295066494 70-71 0.1954915039081202 72-73 0.2144883604498784 74-75 0.24524924696529066 76-77 0.24516375256141235 78-79 0.2398972972825091 80-81 0.2454373346538229 82-83 0.23550288492316446 84-85 0.2066399741738505 86-87 0.21744646682406754 88-89 0.21907086049775523 90-91 0.20737522604720385 92-93 0.20381865884586658 94-95 0.24444559956883463 96-97 0.27291523606030843 98-99 0.28984312802821177 100-101 0.2854487156688672 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2924168.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.63118398384121 #Duplication Level Percentage of deduplicated Percentage of total 1 83.24367768863242 60.46086869694639 2 10.53472078814985 15.302984875650166 3 3.0566696875447423 6.660286153616779 4 1.243026684651834 3.6112999931908614 5 0.6239469934259269 2.265900443784153 6 0.3646766653318713 1.58921387845997 7 0.21856985694878855 1.111249124535852 8 0.14626889232026544 0.8498946263380686 9 0.10408063171500641 0.6803549560122344 >10 0.4430999337765661 5.350725972823551 >50 0.014981438623069835 0.7380476564404828 >100 0.0055638837438425175 0.6810796326984448 >500 3.584275679454237E-4 0.18520633254308352 >1k 3.5842756785915117E-4 0.5128876569599659 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3153 0.10782554217131164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.839552310263979E-5 2 0.0 0.0 0.0 0.0 6.839552310263979E-5 3 0.0 0.0 0.0 0.0 6.839552310263979E-5 4 0.0 0.0 0.0 3.4197761551319894E-5 6.839552310263979E-5 5 0.0 0.0 0.0 3.4197761551319894E-5 6.839552310263979E-5 6 0.0 0.0 0.0 3.4197761551319894E-5 6.839552310263979E-5 7 3.4197761551319894E-5 0.0 0.0 3.4197761551319894E-5 6.839552310263979E-5 8 3.4197761551319894E-5 0.0 0.0 3.4197761551319894E-5 6.839552310263979E-5 9 3.4197761551319894E-5 0.0 0.0 6.839552310263979E-5 6.839552310263979E-5 10-11 3.4197761551319894E-5 0.0 0.0 1.0259328465395969E-4 6.839552310263979E-5 12-13 1.0259328465395969E-4 0.0 0.0 1.0259328465395969E-4 6.839552310263979E-5 14-15 1.3679104620527958E-4 0.0 0.0 1.1969216542961963E-4 1.1969216542961963E-4 16-17 1.5388992698093954E-4 0.0 0.0 1.3679104620527958E-4 2.0518656930791938E-4 18-19 1.8808768853225945E-4 0.0 0.0 1.7098880775659947E-4 2.3938433085923927E-4 20-21 2.0518656930791938E-4 0.0 0.0 2.7358209241055916E-4 2.3938433085923927E-4 22-23 2.0518656930791938E-4 0.0 0.0 3.0777985396187907E-4 2.564832116348992E-4 24-25 2.0518656930791938E-4 0.0 0.0 3.761753770645189E-4 3.2487873473753905E-4 26-27 2.0518656930791938E-4 0.0 0.0 5.813619463724382E-4 3.41977615513199E-4 28-29 2.7358209241055916E-4 0.0 0.0 0.0017953824814442945 3.41977615513199E-4 30-31 2.7358209241055916E-4 0.0 0.0 0.00663436574095606 3.41977615513199E-4 32-33 2.7358209241055916E-4 0.0 0.0 0.01675690316014675 3.7617537706451885E-4 34-35 2.7358209241055916E-4 0.0 0.0 0.0319749070504841 4.1037313861583876E-4 36-37 2.7358209241055916E-4 0.0 0.0 0.055195187143830315 4.1037313861583876E-4 38-39 4.1037313861583876E-4 0.0 0.0 0.09035048601858717 4.2747201939149874E-4 40-41 4.1037313861583876E-4 0.0 0.0 0.13219144727662707 4.445709001671587E-4 42-43 4.616697809428186E-4 0.0 0.0 0.17591328541998955 4.7876866171847854E-4 44-45 4.7876866171847854E-4 0.0 0.0 0.2328012617606102 4.958675424941385E-4 46-47 5.471641848211184E-4 0.0 0.0 0.29868324938922797 5.300653040454584E-4 48-49 5.813619463724383E-4 0.0 0.0 0.3640180728330247 5.642630655967783E-4 50-51 5.813619463724383E-4 0.0 0.0 0.43826141316094014 5.984608271480982E-4 52-53 5.813619463724383E-4 0.0 0.0 0.5140607516394406 6.66856350250738E-4 54-55 5.984608271480982E-4 0.0 0.0 0.6002220118679912 7.010541118020578E-4 56-57 6.83955231026398E-4 0.0 0.0 0.7032769663028937 7.181529925777178E-4 58-59 6.83955231026398E-4 0.0 0.0 0.8147787678409721 7.181529925777178E-4 60-61 6.83955231026398E-4 0.0 0.0 0.9361637224673822 7.181529925777178E-4 62-63 6.83955231026398E-4 0.0 0.0 1.0593953562175633 7.865485156803577E-4 64-65 6.83955231026398E-4 0.0 0.0 1.1896375310857654 7.865485156803577E-4 66-67 7.181529925777178E-4 0.0 0.0 1.3439549300860962 8.036473964560175E-4 68-69 7.181529925777178E-4 0.0 0.0 1.5196972266983293 8.207462772316775E-4 70-71 7.352518733533778E-4 0.0 0.0 1.7150690384410199 8.549440387829974E-4 72-73 7.523507541290377E-4 0.0 0.0 1.9167161394283774 0.0010601306080909169 74-75 7.523507541290377E-4 0.0 0.0 2.1257328580300445 0.0010943283696422366 76-77 7.523507541290377E-4 0.0 0.0 2.3491468342448174 0.0011114272504178966 78-79 7.523507541290377E-4 0.0 0.0 2.612503795951532 0.0011285261311935566 80-81 8.549440387829974E-4 0.0 0.0 2.8997136963402923 0.0011456250119692166 82-83 8.549440387829974E-4 0.0 0.0 3.19165998670391 0.0011969216542961963 84-85 8.891418003343172E-4 0.0 0.0 3.492121519693807 0.0011969216542961963 86-87 9.233395618856372E-4 3.4197761551319894E-5 0.0 3.807339386793098 0.0012140205350718563 88-89 9.233395618856372E-4 3.4197761551319894E-5 0.0 4.152599987415224 0.0012653171773988363 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7625 0.0 25.46243 1 TACATGG 8240 0.0 23.217173 2 ACATGGG 8100 0.0 22.568748 3 GTATCAA 15705 0.0 21.80031 1 GTATAGG 850 0.0 20.876453 1 GAGTACT 6615 0.0 20.153805 12-13 AGAGTAC 10755 0.0 19.855137 10-11 GTCTAGA 1215 0.0 19.759607 1 GTATAAT 1800 0.0 19.71665 1 TATCAAC 18805 0.0 18.764145 2 GTACTTT 7020 0.0 18.690174 14-15 AGTACTT 7160 0.0 18.55384 12-13 CATGGGG 4415 0.0 18.507057 4 ATCAACG 19690 0.0 17.96874 3 TCAACGC 19840 0.0 17.782831 4 CAACGCA 19740 0.0 17.76229 5 GGTATCA 13240 0.0 17.581003 1 AACGCAG 20215 0.0 17.414658 6 TTTGCGT 465 0.0 17.269411 3 GTATAGC 1245 0.0 16.768236 1 >>END_MODULE