FastQCFastQC Report
Fri 27 May 2016
SRR939606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2924168
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82620.28254190593700496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72490.24789957348551792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55000.18808768853225943No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43880.1500597776871917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38660.1322085461574027No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT36060.12331712815405954No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31980.10936444144112104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG85200.021.8087162
GTACATG88700.021.6430551
ACATGGG82950.021.033273
AGAGTAC115850.020.09451510-11
GTATCAA171100.019.9037531
GAGTACT73000.019.23711812-13
AGTACTT78600.018.52603712-13
GTACTTT77800.017.89882514-15
CATGGGG46350.017.7019424
AACGCAG192200.017.4813656
CAACGCA193700.017.3572675
TCAACGC193150.017.3334544
ATCAACG193900.017.2420883
TATCAAC201350.016.7466472
ACGCATA6000.016.21585864-65
ACGCAGA207900.015.9572247
CGCAGAG206800.015.9281678
CATGGGA41600.015.189134
GGTATCA136650.015.0495891
ACTTTTT95950.014.83204816-17
Produced by FastQC (version 0.11.5)