##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939606_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2924168 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.459127177371478 31.0 31.0 34.0 30.0 34.0 2 31.67182938873553 31.0 31.0 34.0 30.0 34.0 3 31.729029590639115 31.0 31.0 34.0 30.0 34.0 4 35.5273547894649 37.0 35.0 37.0 33.0 37.0 5 35.279104004968254 37.0 35.0 37.0 33.0 37.0 6 35.27753466969066 37.0 35.0 37.0 32.0 37.0 7 35.1875904530793 37.0 35.0 37.0 32.0 37.0 8 35.1473242987407 37.0 35.0 37.0 32.0 37.0 9 36.75040797929531 39.0 35.0 39.0 32.0 39.0 10-11 36.62698364115879 39.0 35.0 39.0 32.0 39.0 12-13 36.56944727525915 39.0 35.0 39.0 32.0 39.0 14-15 37.64811255714446 39.0 36.0 41.0 32.0 41.0 16-17 37.59362133092216 39.0 36.0 41.0 32.0 41.0 18-19 37.55721456496344 39.0 36.0 41.0 32.0 41.0 20-21 37.486326538010125 39.0 36.0 41.0 32.0 41.0 22-23 37.35069667679832 39.0 36.0 41.0 31.5 41.0 24-25 37.21672916877553 39.0 36.0 40.5 31.0 41.0 26-27 36.93301342467328 39.0 36.0 40.0 31.0 41.0 28-29 36.74102975615628 39.0 36.0 40.0 30.0 41.0 30-31 36.515820226471256 38.0 35.0 40.0 30.0 41.0 32-33 36.36038438967939 38.0 35.0 40.0 30.0 41.0 34-35 36.337157953988964 38.0 35.0 40.0 30.0 41.0 36-37 36.27697673321095 38.0 35.0 40.0 30.0 41.0 38-39 36.33587228914344 38.0 35.0 40.0 30.0 41.0 40-41 36.25082296913173 38.0 35.0 40.0 30.0 41.0 42-43 36.082361888920204 38.0 35.0 40.0 29.5 41.0 44-45 35.891908741221435 38.0 35.0 40.0 29.0 41.0 46-47 35.64268725326315 38.0 34.5 40.0 28.0 41.0 48-49 35.37250031461941 38.0 34.0 40.0 27.0 41.0 50-51 35.14141270269013 38.0 34.0 40.0 26.5 41.0 52-53 34.92877580905065 38.0 34.0 40.0 26.0 41.0 54-55 34.653613609067605 38.0 33.0 40.0 26.0 41.0 56-57 33.95133248158108 37.5 32.5 40.0 23.5 41.0 58-59 30.866889829859296 35.5 27.5 39.0 8.5 40.5 60-61 30.620875407979295 34.5 26.5 39.0 10.5 40.0 62-63 32.3349807534998 35.5 30.5 39.0 20.5 40.0 64-65 32.36014979303515 36.0 31.0 39.0 19.0 40.0 66-67 27.530401126063893 32.0 19.0 37.0 5.0 39.5 68-69 19.7930216389756 20.0 8.0 32.0 2.0 37.0 70-71 17.815036105996647 15.5 7.5 29.0 2.0 35.0 72-73 19.55607714741424 20.0 7.5 31.0 2.0 35.5 74-75 21.09130511653229 23.5 9.5 32.5 2.0 36.0 76-77 23.070717038145553 27.5 12.5 33.0 2.0 35.5 78-79 25.375938899543392 30.5 18.0 34.5 2.0 36.5 80-81 26.97895452655251 32.5 24.0 35.0 2.0 37.0 82-83 27.970003775432875 33.0 26.5 35.0 2.0 36.0 84-85 27.968270632877456 34.0 27.5 35.0 2.0 36.0 86-87 27.691128553489403 33.5 27.0 35.0 2.0 36.0 88-89 27.242764266622164 33.0 26.0 35.0 2.0 35.5 90-91 25.504106296218275 32.5 17.5 34.5 2.0 35.0 92-93 23.991006672667233 31.5 4.5 34.0 2.0 35.0 94-95 20.07445280161742 28.0 2.0 34.0 2.0 35.0 96-97 17.31441387772522 18.5 2.0 33.0 2.0 35.0 98-99 17.584542851163135 22.0 2.0 33.0 2.0 35.0 100-101 16.775019595317367 18.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2909293329497409 1101 2 0.2486673649880906 1101 3 0.23616392818070508 1101 4 0.23799400345214394 1101 5 0.34676610385666606 1101 6 0.35006043434580647 1101 7 0.3448413177151153 1101 8 0.3618694159457263 1101 9 0.4268850419465835 1101 10-11 0.314702421466194 1101 12-13 0.3001207505189498 1101 14-15 0.5002124300839341 1101 16-17 0.42224097326234045 1101 18-19 0.4439263090114025 1101 20-21 0.5196817491836825 1101 22-23 0.5611608666230836 1101 24-25 0.4795646064872088 1101 26-27 0.5089938671471899 1101 28-29 0.44351463859386087 1101 30-31 0.42193132753983775 1101 32-33 0.5727181337261058 1101 34-35 0.3952949544062605 1101 36-37 0.3078096526370828 1101 38-39 0.2843664861025914 1101 40-41 0.19632875826263074 1101 42-43 0.41631313197675723 1101 44-45 0.3326979073465708 1101 46-47 0.3843963260848966 1101 48-49 0.25091518222777864 1101 50-51 0.2750103382073448 1101 52-53 0.12019145410044985 1101 54-55 0.15817303939817862 1101 56-57 -3.5636249643092626 1101 58-59 -10.46340649790087 1101 60-61 -12.040256274175828 1101 62-63 -4.156582949187417 1101 64-65 -1.2152524017384039 1101 66-67 -2.074712830480429 1101 68-69 -4.770855293691678 1101 70-71 -4.603555298066144 1101 72-73 -3.554052784447073 1101 74-75 -6.149597141017393 1101 76-77 -5.095916089508595 1101 78-79 1.340874832909794 1101 80-81 2.1070027336973105 1101 82-83 0.7617038856153506 1101 84-85 0.4517141680741368 1101 86-87 0.28581887151269214 1101 88-89 0.2688874140606856 1101 90-91 1.6495773271726755 1101 92-93 1.0473804436216376 1101 94-95 -8.581071011938539 1101 96-97 -9.77541431746833 1101 98-99 -8.930004998663557 1101 100-101 -9.68355269631751 1102 1 0.2906360821658005 1102 2 0.2829647567546232 1102 3 0.2829305309609289 1102 4 0.21686512024871973 1102 5 0.28661647369986554 1102 6 0.24182199227414714 1102 7 0.3001504612839412 1102 8 0.2940467499967525 1102 9 0.31332144715929644 1102 10-11 0.32694974720673287 1102 12-13 0.3513465950999759 1102 14-15 0.4481065985504529 1102 16-17 0.38391501952634144 1102 18-19 0.43561309502784695 1102 20-21 0.3577377339019918 1102 22-23 0.33387902831508853 1102 24-25 0.28156993511201733 1102 26-27 0.09720238919327073 1102 28-29 0.3753443414355715 1102 30-31 0.3579483231353535 1102 32-33 0.1847529300664803 1102 34-35 0.06665133452984406 1102 36-37 -0.12482510571676642 1102 38-39 -0.010206037772242382 1102 40-41 0.01117401381215899 1102 42-43 0.13778588339543063 1102 44-45 0.09924471186081263 1102 46-47 -0.044742507563363176 1102 48-49 0.07939875755634773 1102 50-51 0.14377169756971142 1102 52-53 -0.04515356647308266 1102 54-55 -0.25757080224151707 1102 56-57 -3.7545846666780207 1102 58-59 -10.241902591518905 1102 60-61 -8.445523461768499 1102 62-63 -2.876205499299985 1102 64-65 -0.8785908362277759 1102 66-67 -2.037018984674223 1102 68-69 -4.888875690433242 1102 70-71 -4.688491047523183 1102 72-73 -3.4994180519931355 1102 74-75 -4.2442844409030585 1102 76-77 -4.201524705469996 1102 78-79 1.6939053287391523 1102 80-81 2.036420789012009 1102 82-83 0.6296478025524657 1102 84-85 0.3383395206319584 1102 86-87 0.1480442104824462 1102 88-89 0.1436987482363321 1102 90-91 1.6163237674365973 1102 92-93 1.048781994688376 1102 94-95 -8.914169275443466 1102 96-97 -9.695289757672947 1102 98-99 -8.801873784092944 1102 100-101 -9.69560605425951 1103 1 0.17321977766259167 1103 2 0.160416268114691 1103 3 0.16559988566890738 1103 4 0.1576379468626925 1103 5 0.19575452466359877 1103 6 0.24109895484641441 1103 7 0.2483327175586183 1103 8 0.31085548200463364 1103 9 0.2654082897570902 1103 10-11 0.3072209571642759 1103 12-13 0.38882434212640504 1103 14-15 0.5202598390107767 1103 16-17 0.37632450813531904 1103 18-19 0.34093350837314773 1103 20-21 0.35647449080372695 1103 22-23 0.35976800513515883 1103 24-25 0.37186137538046893 1103 26-27 0.430355561863081 1103 28-29 0.4184796490939817 1103 30-31 0.3778546027476466 1103 32-33 0.24921074962875878 1103 34-35 0.3400776550817213 1103 36-37 0.333402212829391 1103 38-39 0.34346463442965813 1103 40-41 0.30754042375908597 1103 42-43 0.29501235234958045 1103 44-45 0.22305294354521266 1103 46-47 0.18933068187723023 1103 48-49 0.1538933497890227 1103 50-51 0.06479749701883719 1103 52-53 0.2517047852447405 1103 54-55 0.2816062209000947 1103 56-57 0.47642873889642345 1103 58-59 -8.728686756833447 1103 60-61 -7.612170325499246 1103 62-63 -2.139493085088148 1103 64-65 -0.2956515352658897 1103 66-67 -1.6685394006721985 1103 68-69 -3.2713747398923196 1103 70-71 -3.8307122332382484 1103 72-73 -2.797306524805453 1103 74-75 -3.58330748009924 1103 76-77 -0.21726877637887299 1103 78-79 3.67882359695675 1103 80-81 3.1480257147298154 1103 82-83 1.6033168057813576 1103 84-85 0.9945648820954069 1103 86-87 0.9917566770108763 1103 88-89 0.7503406897861709 1103 90-91 2.21243349449135 1103 92-93 1.7752475408957196 1103 94-95 -7.298185414007278 1103 96-97 -8.92650957431434 1103 98-99 -8.312049269421456 1103 100-101 -9.426471352294618 1104 1 0.11144769148105738 1104 2 0.13769097866130764 1104 3 0.12820079623430303 1104 4 0.15257644779884316 1104 5 0.2209184209114241 1104 6 0.14150121634768453 1104 7 0.23783599364256247 1104 8 0.1527311804392184 1104 9 0.18895283032290422 1104 10-11 0.11511108178248719 1104 12-13 0.16794457689248077 1104 14-15 0.27401845265379166 1104 16-17 0.18588793013801563 1104 18-19 0.19062256115316956 1104 20-21 0.24489920899054596 1104 22-23 0.2232618073624124 1104 24-25 0.2707641311939284 1104 26-27 0.17345387751961283 1104 28-29 0.23558892614629912 1104 30-31 0.2656005391700802 1104 32-33 0.3483803603832314 1104 34-35 0.3156245742372832 1104 36-37 0.16139142080687918 1104 38-39 0.050836973934686114 1104 40-41 0.1316734443655534 1104 42-43 -0.009927455706325361 1104 44-45 0.001972231662023205 1104 46-47 -0.16107296038434527 1104 48-49 -0.21693293399587077 1104 50-51 -0.11841991381626116 1104 52-53 -0.14114895173698727 1104 54-55 -0.11207090947497278 1104 56-57 0.36090844079947715 1104 58-59 -8.649473642924406 1104 60-61 -7.848552899199628 1104 62-63 -2.3683842056947952 1104 64-65 -0.5649405725813956 1104 66-67 -1.7962582557825755 1104 68-69 -3.4117594013022785 1104 70-71 -3.9496987919969637 1104 72-73 -2.8684205590372613 1104 74-75 -3.683253354295406 1104 76-77 -0.3002866213250144 1104 78-79 3.405008243270398 1104 80-81 2.836747296381535 1104 82-83 1.2293245306749938 1104 84-85 0.7156020540037495 1104 86-87 0.7792182688618148 1104 88-89 0.6809167982145148 1104 90-91 1.9372249536604258 1104 92-93 1.4958853048740757 1104 94-95 -6.385902722666119 1104 96-97 -7.971776420050276 1104 98-99 -5.999403069918161 1104 100-101 -5.419935937633889 1105 1 0.22287098656086357 1105 2 0.2863624894651906 1105 3 0.20696442797135362 1105 4 0.15913652099415287 1105 5 0.1694599875940881 1105 6 0.18690644273953438 1105 7 0.1375726439656404 1105 8 0.16911611440534102 1105 9 0.23371806554608554 1105 10-11 0.2481592209808099 1105 12-13 0.2089663175075529 1105 14-15 0.2743223572454383 1105 16-17 0.3456502979837168 1105 18-19 0.3641657883888598 1105 20-21 0.23721005329054634 1105 22-23 0.42249023490757054 1105 24-25 0.39621947354383025 1105 26-27 0.23927249274000673 1105 28-29 0.32202290957445 1105 30-31 0.35119919426246327 1105 32-33 0.3070158442028941 1105 34-35 0.3570858702544797 1105 36-37 0.2570704747313499 1105 38-39 0.34745342173578564 1105 40-41 0.23350674168126062 1105 42-43 0.24606512933971914 1105 44-45 0.36009911849463094 1105 46-47 0.24132403621315746 1105 48-49 0.1388507955781506 1105 50-51 0.2440803551465507 1105 52-53 0.26542936415351903 1105 54-55 0.20217200464122698 1105 56-57 0.7545842129098617 1105 58-59 -8.291773073845867 1105 60-61 -7.720499282515139 1105 62-63 -2.181768777613584 1105 64-65 -0.3605799603378337 1105 66-67 -1.497700598107219 1105 68-69 -3.4255471367850703 1105 70-71 -3.7879730891620476 1105 72-73 -2.840798218377852 1105 74-75 -3.7157985564197915 1105 76-77 -0.15302648704006927 1105 78-79 3.6951187846481055 1105 80-81 3.146163146570654 1105 82-83 1.677274613047775 1105 84-85 1.2768504998664447 1105 86-87 1.0725681515940266 1105 88-89 1.076609183339329 1105 90-91 2.4743829849836025 1105 92-93 2.1756940091656 1105 94-95 -6.319506515649884 1105 96-97 -7.746612680166786 1105 98-99 -5.66129473450329 1105 100-101 -5.062677870854399 1106 1 0.21839821542322468 1106 2 0.2673645719572555 1106 3 0.2782055469443243 1106 4 0.24472874521399035 1106 5 0.31870496633620604 1106 6 0.304365339455984 1106 7 0.26546029056099485 1106 8 0.2941873812446758 1106 9 0.2343747921497723 1106 10-11 0.2861117600521794 1106 12-13 0.27596443391962566 1106 14-15 0.20750771275658053 1106 16-17 0.421599958089125 1106 18-19 0.3526782168969689 1106 20-21 0.35419526665381795 1106 22-23 0.39864960170653063 1106 24-25 0.252927472833953 1106 26-27 0.4348004074787184 1106 28-29 0.521867558581512 1106 30-31 0.5640733505273801 1106 32-33 0.3596907190947576 1106 34-35 0.46591601383412495 1106 36-37 0.391704572559604 1106 38-39 0.49476208581155134 1106 40-41 0.4777078714910701 1106 42-43 0.49265782424220106 1106 44-45 0.5075561033307636 1106 46-47 0.45169045707888955 1106 48-49 0.5241654113459404 1106 50-51 0.35141528828646784 1106 52-53 0.45500344351455624 1106 54-55 0.3460975836280298 1106 56-57 1.0804629276804647 1106 58-59 -8.192955010263415 1106 60-61 -7.698137141517822 1106 62-63 -1.937289867882626 1106 64-65 0.011793883424672913 1106 66-67 1.2600470182581347 1106 68-69 -1.7612244525393912 1106 70-71 -2.3973600786021247 1106 72-73 -0.7723923407604794 1106 74-75 -0.7703072931984529 1106 76-77 2.673099571888713 1106 78-79 4.794550707562461 1106 80-81 3.84867242519163 1106 82-83 2.2026318903244366 1106 84-85 1.6868318011624943 1106 86-87 1.674108715607293 1106 88-89 1.67712479088431 1106 90-91 2.9069304754906433 1106 92-93 2.5512914717660493 1106 94-95 -5.779354456863423 1106 96-97 -7.495035378901601 1106 98-99 -5.162417428351237 1106 100-101 -4.485828426714312 1107 1 0.049049467942936076 1107 2 0.08632579142226149 1107 3 0.1107918218408841 1107 4 0.10814340835001701 1107 5 0.10161588785984321 1107 6 0.11859545609407007 1107 7 0.08239706137248959 1107 8 0.17678030179650506 1107 9 0.1510552548005819 1107 10-11 0.09815432884650477 1107 12-13 0.09826041395379548 1107 14-15 0.1693425022383508 1107 16-17 0.10809421315833134 1107 18-19 0.1569072119333299 1107 20-21 0.18434109091364093 1107 22-23 0.12220798267963318 1107 24-25 0.16458695191079187 1107 26-27 0.1555578027629565 1107 28-29 0.0673692140524409 1107 30-31 -0.012553129970378052 1107 32-33 -0.02643873713782341 1107 34-35 0.03619511531888975 1107 36-37 0.04790171448448177 1107 38-39 0.2696902500042384 1107 40-41 0.22283175754200357 1107 42-43 0.1320465050829327 1107 44-45 0.025057759556347037 1107 46-47 0.09722493048405312 1107 48-49 0.11208983595435029 1107 50-51 0.11409672198589504 1107 52-53 0.09821335254973462 1107 54-55 0.03679638300480548 1107 56-57 0.6325981639823226 1107 58-59 -8.478284591843714 1107 60-61 -7.872791376422729 1107 62-63 -2.295913574845173 1107 64-65 -0.40996321839421057 1107 66-67 0.43627885342776906 1107 68-69 -2.7175084454905623 1107 70-71 -2.775108792839987 1107 72-73 -1.366207485515492 1107 74-75 -1.0986803452880345 1107 76-77 2.019975892235145 1107 78-79 4.225877269968276 1107 80-81 3.2231749089875983 1107 82-83 1.575006679623204 1107 84-85 1.1011624185971662 1107 86-87 0.8352678367432063 1107 88-89 0.8202254640245705 1107 90-91 2.3938528258433642 1107 92-93 1.800795217246037 1107 94-95 -6.972532382798278 1107 96-97 -8.224703376302957 1107 98-99 -6.0622213225637225 1107 100-101 -5.40646902071609 1108 1 -0.0884518586783507 1108 2 -0.07852065921004936 1108 3 -0.08744029397634634 1108 4 -0.18306373176569934 1108 5 -0.20260821409325303 1108 6 -0.08774985157072024 1108 7 -0.26217785189513165 1108 8 -0.17189947358124158 1108 9 -0.16589947310065867 1108 10-11 -0.15483922295872077 1108 12-13 -0.18133838222416188 1108 14-15 -0.1510603228802978 1108 16-17 -0.13899003863797788 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2.7545609534828905 2314 62-63 0.09227133430411527 2314 64-65 -0.7509375161082481 2314 66-67 -2.774677614450841 2314 68-69 -5.136888811470799 2314 70-71 -3.6921866895316207 2314 72-73 -3.126490062708987 2314 74-75 -4.6564234747264805 2314 76-77 -0.04630840105166811 2314 78-79 2.791119757430611 2314 80-81 2.0628310535445564 2314 82-83 0.333916401059426 2314 84-85 0.0021628998666720634 2314 86-87 0.08343100090032252 2314 88-89 0.0876327478411234 2314 90-91 1.8090664881531602 2314 92-93 3.307130845947082 2314 94-95 5.8567836948722345 2314 96-97 3.14239613689114 2314 98-99 5.54949381085866 2314 100-101 5.974854207784135 2315 1 -0.462229850489166 2315 2 -0.4272750879653735 2315 3 -0.48520878328840666 2315 4 -0.3938768912855295 2315 5 -0.5308995587218135 2315 6 -0.6285848470440385 2315 7 -0.5467116849372928 2315 8 -0.6626213964079071 2315 9 -0.8430564162685954 2315 10-11 -0.6845569979322903 2315 12-13 -0.6752711072676831 2315 14-15 -0.9233298917884767 2315 16-17 -0.941204515558475 2315 18-19 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2315 84-85 0.012495482319071272 2315 86-87 0.14436866076363586 2315 88-89 0.30031779161654626 2315 90-91 1.9139635757026277 2315 92-93 3.5423588624529785 2315 94-95 6.139900794004031 2315 96-97 3.5819491745419505 2315 98-99 5.766172921293535 2315 100-101 6.255603390707725 2316 1 -0.48990686793801785 2316 2 -0.4213021291993364 2316 3 -0.532755169937861 2316 4 -0.3773624152903281 2316 5 -0.5232395030680479 2316 6 -0.4598524517184046 2316 7 -0.4433821095384829 2316 8 -0.4815672900980985 2316 9 -0.7026720170328957 2316 10-11 -0.6362505094212381 2316 12-13 -0.5583164647426244 2316 14-15 -0.6078155259200599 2316 16-17 -0.6905394973875616 2316 18-19 -0.6205730304130483 2316 20-21 -0.6193255466549346 2316 22-23 -0.9037694127710765 2316 24-25 -0.7100902504497455 2316 26-27 -0.6504287372346624 2316 28-29 -0.5978270058969528 2316 30-31 -0.6595652277252668 2316 32-33 -0.6682447593304275 2316 34-35 -0.6307597018999118 2316 36-37 -0.4768729267574088 2316 38-39 -0.6438978535043276 2316 40-41 -0.7802962872678663 2316 42-43 -0.7441890341339956 2316 44-45 -0.5881461796300371 2316 46-47 -0.6642025007136141 2316 48-49 -0.7079998934224747 2316 50-51 -0.6863557185743119 2316 52-53 -0.7601038700742038 2316 54-55 -0.4851087196781876 2316 56-57 0.20168140229996823 2316 58-59 3.4292473348700163 2316 60-61 3.449921302569013 2316 62-63 0.8713799918250658 2316 64-65 0.2231277268458598 2316 66-67 -1.8460793909503117 2316 68-69 -3.1172167594706863 2316 70-71 -3.1658791265712587 2316 72-73 -0.6778155185351729 2316 74-75 5.650875511576768 2316 76-77 5.8273035761690934 2316 78-79 4.882197777597884 2316 80-81 3.2325348612150897 2316 82-83 1.4843106602321576 2316 84-85 1.1303844994999857 2316 86-87 0.8926318932912238 2316 88-89 0.8660061464473081 2316 90-91 2.332026979210564 2316 92-93 3.922045920739567 2316 94-95 6.6390510012580926 2316 96-97 3.9265585820939215 2316 98-99 6.263835849326249 2316 100-101 6.342504775012726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 16.0 4 48.0 5 90.0 6 221.0 7 613.0 8 1502.0 9 2494.0 10 3904.0 11 6004.0 12 8830.0 13 12133.0 14 15252.0 15 20257.0 16 26893.0 17 35186.0 18 39236.0 19 40588.0 20 42579.0 21 44221.0 22 44558.0 23 45145.0 24 47936.0 25 51719.0 26 64562.0 27 90701.0 28 126427.0 29 166060.0 30 203920.0 31 232771.0 32 245607.0 33 245929.0 34 251455.0 35 265938.0 36 265006.0 37 197253.0 38 74570.0 39 4541.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.38634270051683 20.152437220213358 14.564657373710975 23.89656270555884 2 15.29611062905018 21.554188759859212 39.393745581504 23.75595502958661 3 18.897399218031577 25.355734759977146 30.596113275646093 25.15075274634519 4 12.784969947007149 17.699735446116637 38.322900736209405 31.192393870666802 5 13.164762526214794 37.977567766675755 35.21725131061139 13.640418396498056 6 30.812661926400946 37.8924877093245 17.659142703155222 13.635707661119334 7 26.940380990421893 33.01595530762938 22.172563272698422 17.8711004292503 8 25.19906516999023 35.60414449511793 20.417465754361583 18.779324580530258 9 25.538614744433286 16.655746181477944 20.856770199249837 36.94886887483893 10-11 24.312283015202958 26.65532554901087 28.63067375061898 20.4017176851672 12-13 25.39047004139297 24.092955671493566 28.461429028701495 22.055145258411965 14-15 22.35368286924746 25.563244200415607 26.68686852451487 25.396204405822058 16-17 21.794117471040902 28.46488786979542 27.297307695267115 22.44368696389656 18-19 21.679319873057562 27.88744459947472 28.798167678923175 21.63506784854454 20-21 22.812283699158186 26.860101745180167 28.507288226941817 21.820326328719826 22-23 22.157499158735067 26.869933601626173 28.526644159979863 22.445923079658897 24-25 22.315736017174117 26.933546043446555 28.601884125994836 22.148833813384496 26-27 22.21052088014155 26.92918254298833 28.77461471065596 22.085681866214163 28-29 21.985628604903592 27.458345046170805 28.59207931762548 21.963947031300123 30-31 22.365294967197748 27.01903224890793 28.32217923371011 22.293493550184216 32-33 22.234254716911767 27.005107511850525 28.50897555135017 22.251662219887542 34-35 22.55601518132989 27.008691167729893 28.384251251146498 22.051042399793715 36-37 22.518144433282735 26.732307787089344 28.67547776205251 22.074070017575416 38-39 22.18512278237585 27.202496888130472 28.570726967630737 22.041653361862938 40-41 22.436264821542526 27.16077883615413 28.390431863986898 22.012524478316443 42-43 22.215534542512223 27.160386285095843 28.094284532457888 22.52979463993405 44-45 22.42783909013024 26.98434158999247 28.15744733722828 22.430371982649003 46-47 22.660290395959017 27.242128292556373 27.843565965445745 22.25401534603887 48-49 22.44888285712868 27.1776067993171 28.074305414501083 22.29920492905314 50-51 22.151037344398343 27.352508876245885 28.172733883731873 22.323719895623903 52-53 22.506235416618903 27.503602323965183 27.563672423427306 22.426489835988612 54-55 22.365352999844323 27.102987441557307 27.805223532068425 22.726436026529946 56-57 22.3948939173025 27.225488251790203 27.8573661567166 22.522251674190695 58-59 21.707324930355615 26.835832193337737 29.320445482886974 22.13639739341967 60-61 21.72158736505055 27.143537398039857 29.00947123580795 22.125404001101646 62-63 22.302598550250814 27.179719379554406 28.172677193467237 22.345004876727543 64-65 22.153057801157345 27.473871545916005 27.863015460083425 22.510055192843225 66-67 22.215528656762267 26.840038660096944 28.4550732037376 22.48935947940319 68-69 21.48879814183756 26.20955931394107 30.21024923615129 22.091393308070074 70-71 22.504179572150576 26.064446181722996 29.210014433584313 22.221359812542115 72-73 21.795475165473775 26.336775610646008 29.504365385188375 22.363383838691842 74-75 21.703061928894606 26.92725545045245 28.865964197790987 22.503718422861958 76-77 20.601020239558327 27.143999472081937 29.16018470929444 23.09479557906529 78-79 22.011839224426065 27.059288473652902 28.184962386101937 22.743909915819096 80-81 22.20193247705857 27.1175266620081 27.6226529350233 23.057887925910023 82-83 22.407794634058845 27.127089762874228 27.631472979214074 22.833642623852857 84-85 22.24629974146068 27.252729026168552 27.373585899347496 23.127385333023266 86-87 22.052990210746803 27.21617577907921 27.590253494635146 23.140580515538836 88-89 22.213173114540616 27.13966844586221 27.270167103942043 23.376991335655134 90-91 22.03958616167406 26.91489783317003 27.760856582129694 23.284659423026213 92-93 22.115301044943735 27.144139822217905 27.59283807375582 23.14772105908254 94-95 20.90691188533437 26.96992617536588 28.66770280188231 23.455459137417435 96-97 20.487683787299115 26.90513330205594 28.926769673314766 23.68041323733018 98-99 21.318024254482744 26.871460879922065 28.119554703393767 23.690960162201424 100-101 20.567240846101953 26.830530205223308 28.589842689764566 24.01238625891017 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 307.0 1 227.0 2 159.0 3 197.0 4 233.0 5 265.0 6 346.0 7 528.5 8 777.5 9 992.5 10 1171.5 11 1321.5 12 1460.0 13 1606.5 14 1828.5 15 2085.5 16 2324.5 17 2624.0 18 2995.0 19 3428.5 20 3987.5 21 4778.5 22 5826.0 23 7332.5 24 9275.5 25 11961.5 26 15934.0 27 20683.0 28 25328.0 29 30319.0 30 35893.5 31 41866.0 32 48483.0 33 55592.5 34 62725.0 35 69543.0 36 76679.5 37 82666.5 38 87453.5 39 91422.0 40 94540.0 41 97318.5 42 99953.0 43 103327.0 44 107013.0 45 110529.5 46 114405.0 47 119245.5 48 122646.5 49 120149.5 50 113788.5 51 107985.0 52 102897.0 53 97732.5 54 91873.0 55 85018.0 56 77319.0 57 69318.0 58 60851.5 59 51908.0 60 42659.5 61 33625.5 62 25560.5 63 18813.5 64 13518.0 65 9619.5 66 6705.5 67 4664.5 68 3195.5 69 2139.5 70 1414.5 71 845.5 72 474.5 73 285.5 74 169.0 75 79.5 76 49.5 77 27.5 78 10.0 79 7.0 80 4.5 81 2.5 82 1.0 83 1.0 84 0.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025135354740220127 2 0.01296095162795024 3 3.0777985396187907E-4 4 0.0 5 0.057349646121563465 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.958675424941385E-4 16-17 1.7098880775659947E-5 18-19 2.7358209241055916E-4 20-21 0.0 22-23 0.0 24-25 1.7098880775659947E-5 26-27 6.839552310263979E-5 28-29 8.891418003343174E-4 30-31 0.004479906763222906 32-33 0.004838983259511765 34-35 0.0017098880775659948 36-37 0.0845710643164141 38-39 0.07834707171407387 40-41 0.08376741691995808 42-43 0.08698200650578215 44-45 0.08915356436429098 46-47 0.08556279940140238 48-49 0.07617551385556506 50-51 0.06986602684934655 52-53 0.0598118849532585 54-55 0.049005392303041406 56-57 0.07128523395372632 58-59 0.7436132260526755 60-61 0.35379294212918 62-63 0.002599029877900312 64-65 0.0019492724084252341 66-67 1.139879788028595 68-69 2.4510390647869755 70-71 2.4077617975437797 72-73 1.8673516706290472 74-75 1.3178449391416636 76-77 0.75927580084318 78-79 0.5744710974198473 80-81 0.04225133439665574 82-83 8.549440387829975E-5 84-85 0.001641492554463355 86-87 0.0014705037467067556 88-89 0.0 90-91 0.33655727030731475 92-93 0.058614963298962305 94-95 0.9846561483471538 96-97 1.7060408293914715 98-99 0.6544767605691602 100-101 0.5894668158601011 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2924168.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.995081513265994 #Duplication Level Percentage of deduplicated Percentage of total 1 71.44379077230535 36.43281934050432 2 14.843075220725998 15.138476615769218 3 5.611261083744456 8.584401490732974 4 2.7285887154984367 5.565784158520821 5 1.4890393832396331 3.7966842362384208 6 0.9115475075834693 2.7890663671460074 7 0.6327955669259636 2.2588623061636017 8 0.44401075184976696 1.8113891586676307 9 0.3186321861091687 1.462380687304848 >10 1.4431523757074627 12.812429438213968 >50 0.0890057017604521 3.1149482230171293 >100 0.04248031594621961 3.825766770889953 >500 0.001209423971024719 0.4276796003723852 >1k 0.0012094239708371696 1.2593968426091002 >5k 2.0157066180619493E-4 0.7199147638497063 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8262 0.28254190593700496 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7249 0.24789957348551792 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5500 0.18808768853225943 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4388 0.1500597776871917 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3866 0.1322085461574027 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3606 0.12331712815405954 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3198 0.10936444144112104 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.4197761551319894E-5 0.0 0.0 0.0 3.4197761551319894E-5 7 3.4197761551319894E-5 0.0 0.0 0.0 3.4197761551319894E-5 8 3.4197761551319894E-5 0.0 0.0 0.0 3.4197761551319894E-5 9 3.4197761551319894E-5 0.0 0.0 3.4197761551319894E-5 3.4197761551319894E-5 10-11 3.4197761551319894E-5 0.0 0.0 6.839552310263979E-5 3.4197761551319894E-5 12-13 3.4197761551319894E-5 0.0 0.0 8.549440387829973E-5 3.4197761551319894E-5 14-15 3.4197761551319894E-5 0.0 0.0 1.1969216542961963E-4 8.549440387829973E-5 16-17 5.1296642326979845E-5 0.0 0.0 1.3679104620527958E-4 1.3679104620527958E-4 18-19 6.839552310263979E-5 0.0 0.0 1.7098880775659947E-4 1.709888077565995E-4 20-21 6.839552310263979E-5 0.0 0.0 2.7358209241055916E-4 2.564832116348992E-4 22-23 6.839552310263979E-5 0.0 0.0 3.0777985396187907E-4 3.4197761551319893E-4 24-25 1.0259328465395969E-4 0.0 0.0 3.761753770645189E-4 4.1037313861583876E-4 26-27 1.0259328465395969E-4 0.0 0.0 5.813619463724382E-4 4.445709001671587E-4 28-29 1.5388992698093954E-4 0.0 0.0 0.0018979757660982544 4.445709001671587E-4 30-31 1.709888077565995E-4 0.0 0.0 0.006976343356469259 4.445709001671587E-4 32-33 1.709888077565995E-4 0.0 0.0 0.017526352795051447 4.445709001671587E-4 34-35 2.906809731862191E-4 0.0 0.0 0.0336847951280501 4.445709001671587E-4 36-37 4.445709001671587E-4 0.0 0.0 0.058324282325776085 4.445709001671587E-4 38-39 5.471641848211183E-4 0.0 0.0 0.09589052338990098 4.445709001671587E-4 40-41 5.471641848211183E-4 0.0 0.0 0.13952686712938517 4.616697809428186E-4 42-43 5.984608271480982E-4 0.0 0.0 0.18499279111186498 4.958675424941385E-4 44-45 6.49757469475078E-4 0.0 0.0 0.2438984353840135 5.300653040454584E-4 46-47 7.694496349046977E-4 0.0 0.0 0.31179809094415917 5.642630655967783E-4 48-49 8.207462772316775E-4 0.0 0.0 0.37514944421797924 5.813619463724383E-4 50-51 8.207462772316775E-4 0.0 0.0 0.44421182367086975 5.813619463724383E-4 52-53 8.207462772316775E-4 0.0 0.0 0.5131716098391064 7.523507541290378E-4 54-55 8.207462772316775E-4 0.0 0.0 0.593741536054016 8.207462772316775E-4 56-57 8.549440387829974E-4 0.0 0.0 0.6708916861137937 8.378451580073375E-4 58-59 8.549440387829974E-4 0.0 0.0 0.7271812016272663 8.549440387829974E-4 60-61 8.549440387829974E-4 0.0 0.0 0.7727155211328487 8.549440387829974E-4 62-63 8.720429195586574E-4 1.7098880775659947E-5 0.0 0.8122481334861745 8.891418003343174E-4 64-65 9.233395618856372E-4 6.839552310263979E-5 0.0 0.8595949343539769 8.891418003343174E-4 66-67 9.575373234369571E-4 6.839552310263979E-5 1.7098880775659947E-5 0.9028722015971723 8.891418003343174E-4 68-69 9.575373234369571E-4 6.839552310263979E-5 3.4197761551319894E-5 0.9549724913206081 8.891418003343174E-4 70-71 9.575373234369571E-4 6.839552310263979E-5 3.4197761551319894E-5 1.0369445257591219 9.233395618856372E-4 72-73 9.74636204212617E-4 6.839552310263979E-5 6.839552310263979E-5 1.1452317377113763 0.0010601306080909169 74-75 9.91735084988277E-4 6.839552310263979E-5 6.839552310263979E-5 1.2933935396324698 0.0011114272504178966 76-77 9.91735084988277E-4 6.839552310263979E-5 6.839552310263979E-5 1.4812760415954214 0.0011285261311935566 78-79 9.91735084988277E-4 6.839552310263979E-5 6.839552310263979E-5 1.7270040572224303 0.0011627238927448766 80-81 0.0010601306080909169 6.839552310263979E-5 6.839552310263979E-5 2.013598397903267 0.0012140205350718563 82-83 0.0010601306080909169 6.839552310263979E-5 6.839552310263979E-5 2.2981750706525754 0.001333712700501476 84-85 0.0010772294888665769 6.839552310263979E-5 6.839552310263979E-5 2.555752610657117 0.001350811581277136 86-87 0.0010943283696422366 6.839552310263979E-5 6.839552310263979E-5 2.795598611297299 0.0013850093428284557 88-89 0.0010943283696422366 6.839552310263979E-5 6.839552310263979E-5 3.0285708618656657 0.0014021082236041157 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 8520 0.0 21.808716 2 GTACATG 8870 0.0 21.643055 1 ACATGGG 8295 0.0 21.03327 3 AGAGTAC 11585 0.0 20.094515 10-11 GTATCAA 17110 0.0 19.903753 1 GAGTACT 7300 0.0 19.237118 12-13 AGTACTT 7860 0.0 18.526037 12-13 GTACTTT 7780 0.0 17.898825 14-15 CATGGGG 4635 0.0 17.701942 4 AACGCAG 19220 0.0 17.481365 6 CAACGCA 19370 0.0 17.357267 5 TCAACGC 19315 0.0 17.333454 4 ATCAACG 19390 0.0 17.242088 3 TATCAAC 20135 0.0 16.746647 2 ACGCATA 600 0.0 16.215858 64-65 ACGCAGA 20790 0.0 15.957224 7 CGCAGAG 20680 0.0 15.928167 8 CATGGGA 4160 0.0 15.18913 4 GGTATCA 13665 0.0 15.049589 1 ACTTTTT 9595 0.0 14.832048 16-17 >>END_MODULE