FastQCFastQC Report
Fri 27 May 2016
SRR939605_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939605_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576883
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117490.45593843414699076No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA113450.44026057838093535No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA88070.3417694943852709No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83120.3225602404144853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60130.2333439275279475No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55410.21502722475176406No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT43990.17071011761108285No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA37430.14525300527808208No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG35650.13834543516333492No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT34840.13520210269538818No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30150.11700181964023977No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG29530.11459581207218178No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC29030.11265548338826403No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG121450.026.3351251
TACATGG122150.026.1852262
ACATGGG125750.023.6996293
CATGGGG44750.021.7391364
GTATAGG5100.021.4008351
GAGTACT89700.021.16080312-13
CATGGGA84350.020.7035224
AGTACTT95000.020.35488312-13
GTACTTT103900.018.38329714-15
ATGGGAG35250.017.5007745
GCTTATA23700.017.2196031
TACTTTT120450.016.15288414-15
ACTTTTT126500.016.0754416-17
ATGGGGA25100.015.6919695
ATGGGAT35150.015.6605035
ATAGGAG15950.015.4709045
ATTCGCG2007.747076E-915.434780-81
TTATAGG16050.015.375113
AGTACAT91650.015.3267172
CTTATAG16450.015.0012472
Produced by FastQC (version 0.11.5)