##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939605_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2576883 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.280641379527125 31.0 30.0 33.0 26.0 34.0 2 30.414286562486538 31.0 30.0 34.0 26.0 34.0 3 30.459469056220247 31.0 30.0 34.0 26.0 34.0 4 34.051058973185825 35.0 35.0 37.0 28.0 37.0 5 34.05560555135798 35.0 35.0 37.0 28.0 37.0 6 34.17548720683089 36.0 35.0 37.0 30.0 37.0 7 33.99247928602114 36.0 35.0 37.0 29.0 37.0 8 34.001800625018674 36.0 35.0 37.0 29.0 37.0 9 35.26450677038888 38.0 35.0 39.0 28.0 39.0 10-11 35.1713005596296 37.0 34.5 39.0 28.5 39.0 12-13 35.01781454571279 37.0 34.0 39.0 27.0 39.0 14-15 35.696930361215465 38.0 34.0 40.0 27.0 41.0 16-17 35.45398898591826 38.0 34.0 40.0 27.0 41.0 18-19 35.46177474879535 38.0 34.0 40.0 27.0 41.0 20-21 35.59486305742247 38.0 34.0 40.0 27.0 41.0 22-23 35.458021571022044 38.0 34.0 40.0 27.0 41.0 24-25 35.3283201061127 38.0 34.0 40.0 26.0 41.0 26-27 35.44657557211561 38.0 34.0 40.0 26.5 41.0 28-29 35.32591933743208 38.0 34.0 40.0 26.0 41.0 30-31 35.10459962675837 38.0 34.0 40.0 25.0 41.0 32-33 34.86851828352316 38.0 34.0 40.0 25.0 41.0 34-35 34.64591485139216 38.0 33.0 40.0 24.0 41.0 36-37 34.34254174520147 38.0 33.0 40.0 24.0 41.0 38-39 34.17154038425493 38.0 33.0 40.0 23.5 41.0 40-41 33.8816750702302 38.0 33.0 40.0 22.0 41.0 42-43 33.563613481869375 38.0 32.0 40.0 20.0 41.0 44-45 33.309965566927175 37.5 32.0 40.0 18.5 41.0 46-47 32.8238812161825 37.0 31.0 40.0 15.5 41.0 48-49 32.450285868625 37.0 31.0 40.0 13.0 41.0 50-51 31.813197766448845 36.5 30.0 39.5 9.0 40.5 52-53 31.820711495244446 36.5 30.0 39.0 9.0 40.0 54-55 31.867337205453254 37.0 30.0 39.0 8.0 40.5 56-57 31.749657823036593 36.0 30.0 39.0 8.0 40.5 58-59 31.36832599695058 36.0 29.5 39.0 6.5 40.0 60-61 30.935826539272448 35.5 29.0 39.0 2.0 40.0 62-63 30.50569622291738 35.0 28.0 38.5 2.0 40.0 64-65 29.862381023895924 34.5 28.0 38.0 2.0 40.0 66-67 29.33147799104577 34.0 27.0 38.0 2.0 40.0 68-69 28.83562136891741 34.0 26.0 37.0 2.0 39.0 70-71 28.275169070539874 33.5 26.0 36.5 2.0 39.0 72-73 27.516708364330086 33.0 25.0 36.0 2.0 38.5 74-75 27.059800736005478 32.0 24.0 35.0 2.0 38.0 76-77 26.673018914712078 32.0 23.5 35.0 2.0 37.5 78-79 26.102288889328698 32.0 21.5 35.0 2.0 37.0 80-81 25.460201724331295 31.0 20.0 34.5 2.0 36.0 82-83 24.815813911613372 31.0 18.0 34.0 2.0 36.0 84-85 24.163113730813546 31.0 14.0 34.0 2.0 35.0 86-87 23.485093424885804 30.0 7.0 34.0 2.0 35.0 88-89 22.674686433183034 29.5 2.0 34.0 2.0 35.0 90-91 21.45593261316094 28.5 2.0 33.5 2.0 35.0 92-93 19.858742131482103 26.0 2.0 33.0 2.0 34.0 94-95 19.60245886212141 26.0 2.0 32.5 2.0 34.0 96-97 18.952960029617177 25.0 2.0 32.0 2.0 34.0 98-99 17.955631668182065 24.5 2.0 32.0 2.0 34.0 100-101 16.327555422578364 18.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9339604041651519 1101 2 0.8463365798241398 1101 3 0.9278488370115809 1101 4 1.1512887566416339 1101 5 0.8218495635541956 1101 6 0.5747173060100934 1101 7 0.8396770516568992 1101 8 0.7900613531458447 1101 9 1.0609948013441368 1101 10-11 0.9530167230873587 1101 12-13 0.8826676114552896 1101 14-15 1.1117348133993872 1101 16-17 0.9279231263315708 1101 18-19 0.7848453946361786 1101 20-21 0.9768941022909914 1101 22-23 1.0657570441830515 1101 24-25 0.7717604138646763 1101 26-27 0.9533312211278897 1101 28-29 0.9707477529142423 1101 30-31 1.1011038362763585 1101 32-33 0.9137695122281997 1101 34-35 1.160107234780405 1101 36-37 1.0767394864699398 1101 38-39 1.0029679515291434 1101 40-41 0.9041293922239575 1101 42-43 0.8113604011848636 1101 44-45 0.7328639623903328 1101 46-47 1.0479352746294879 1101 48-49 0.807263805723089 1101 50-51 0.9665664574300159 1101 52-53 1.1211308527513886 1101 54-55 1.206600036723021 1101 56-57 1.2518357230726487 1101 58-59 1.340815442235371 1101 60-61 0.875975096586366 1101 62-63 0.8753416758823285 1101 64-65 0.8371753173156549 1101 66-67 0.9476820816757687 1101 68-69 0.8218584469483154 1101 70-71 0.8367451011930847 1101 72-73 0.9676501892464984 1101 74-75 0.6917898265750893 1101 76-77 0.2617322633386472 1101 78-79 0.3598378687076753 1101 80-81 0.40474488762871985 1101 82-83 0.36527903397785266 1101 84-85 0.17047665947723445 1101 86-87 -0.21732054187157246 1101 88-89 -0.7139380691937411 1101 90-91 -4.068292416302327 1101 92-93 -9.4068513486734 1101 94-95 -7.583256957749873 1101 96-97 -4.304273118102881 1101 98-99 -3.035704522178424 1101 100-101 -2.738889176026227 1102 1 1.0170773585570103 1102 2 0.8244334239821214 1102 3 0.8384090796886134 1102 4 0.8563846321727269 1102 5 0.9391339585401468 1102 6 0.9149412662329581 1102 7 0.9158375686000255 1102 8 0.9393001007875341 1102 9 1.0848530297191274 1102 10-11 0.8418370296125133 1102 12-13 0.7660859877115982 1102 14-15 0.9793132176525106 1102 16-17 1.1227080077692193 1102 18-19 1.005458188351433 1102 20-21 1.1507881107028695 1102 22-23 1.2879626553766386 1102 24-25 1.1570731007387494 1102 26-27 1.0838720485020445 1102 28-29 1.178203186454958 1102 30-31 1.141728789502153 1102 32-33 1.2103646354519455 1102 34-35 1.0856077811030787 1102 36-37 0.9398507317363922 1102 38-39 0.9858060069482804 1102 40-41 0.9785094603200619 1102 42-43 0.7740926774153039 1102 44-45 0.6085748411608662 1102 46-47 0.6417919420825555 1102 48-49 0.7007636706570182 1102 50-51 0.4992675936243778 1102 52-53 0.652690698426813 1102 54-55 0.6397040190696295 1102 56-57 0.508815503075315 1102 58-59 0.42687195473664374 1102 60-61 0.44479607309627056 1102 62-63 0.4195038278733598 1102 64-65 0.41130806125246977 1102 66-67 0.5778243037586073 1102 68-69 0.6262578234348446 1102 70-71 0.522246595472911 1102 72-73 0.3341468175838642 1102 74-75 0.5405024579972313 1102 76-77 0.38599643832726116 1102 78-79 0.1956857237229741 1102 80-81 0.18356189412258317 1102 82-83 0.10418971716985936 1102 84-85 -0.20184937078468934 1102 86-87 -0.45993235626868767 1102 88-89 -1.1887391585604412 1102 90-91 -5.315484716894549 1102 92-93 -10.582492128507141 1102 94-95 -9.17692545927195 1102 96-97 -5.808774310889568 1102 98-99 -4.424160257212284 1102 100-101 -3.796289228912258 1103 1 0.8933373888233014 1103 2 0.9371032737360991 1103 3 0.9705366088118517 1103 4 0.9039622602627944 1103 5 0.9492322213087121 1103 6 0.7064487680570224 1103 7 0.8051173004529275 1103 8 0.7519644605331166 1103 9 0.8545419390220985 1103 10-11 0.8582444016460684 1103 12-13 0.8807110767146966 1103 14-15 1.024622026433839 1103 16-17 0.9491032556850101 1103 18-19 0.9601090426763719 1103 20-21 0.9886902695334214 1103 22-23 0.9755400026800274 1103 24-25 0.8102090270603313 1103 26-27 0.8639913451271966 1103 28-29 1.057296647771146 1103 30-31 0.9212663966882815 1103 32-33 0.9600414164462876 1103 34-35 1.0137259696685206 1103 36-37 0.9928292079698977 1103 38-39 0.9186432977674386 1103 40-41 0.6352240548997017 1103 42-43 0.8368779430709381 1103 44-45 0.7323499092306847 1103 46-47 0.8648369438070915 1103 48-49 0.732297482612914 1103 50-51 0.7600609082190424 1103 52-53 0.8805141028774095 1103 54-55 0.7688859072385803 1103 56-57 0.5098192107168593 1103 58-59 0.3844131308473102 1103 60-61 0.2913870083799708 1103 62-63 0.5299121922992818 1103 64-65 0.3395362595281064 1103 66-67 0.5088059926947324 1103 68-69 0.7162625297550171 1103 70-71 0.8022821799471664 1103 72-73 0.8070121367817507 1103 74-75 0.48848536185964164 1103 76-77 0.23547140912754116 1103 78-79 0.33355565494699846 1103 80-81 0.17224619056124624 1103 82-83 0.13313348146326476 1103 84-85 -0.06770510174578348 1103 86-87 -0.23994457677592607 1103 88-89 -0.5209716264700859 1103 90-91 -2.2204905987470553 1103 92-93 -9.167305424217853 1103 94-95 -5.532550811041094 1103 96-97 -2.977322492837665 1103 98-99 -2.029840613836523 1103 100-101 -1.6054788533831115 1104 1 0.8251617727303469 1104 2 0.7099272990496424 1104 3 0.8226920414282226 1104 4 1.023519438635617 1104 5 0.9393293272261047 1104 6 0.7933118417220513 1104 7 0.75952751187274 1104 8 0.9306949793961152 1104 9 1.0444628304389312 1104 10-11 0.9024340216798024 1104 12-13 0.8761224330776969 1104 14-15 0.9726248410545324 1104 16-17 1.076205707427114 1104 18-19 1.064244294823233 1104 20-21 1.1699161330709344 1104 22-23 1.0785124195476001 1104 24-25 1.0268837752367261 1104 26-27 1.0566679937739707 1104 28-29 1.0560872028543358 1104 30-31 1.2287222848010941 1104 32-33 1.2591683126879687 1104 34-35 1.1146181683428509 1104 36-37 1.0164649551562164 1104 38-39 1.030290670299479 1104 40-41 0.8434480247327443 1104 42-43 0.8982191579436289 1104 44-45 0.9009810217651406 1104 46-47 0.6310665323567619 1104 48-49 0.824608170428327 1104 50-51 0.9202675543022885 1104 52-53 0.9823169937274123 1104 54-55 0.9823017396303264 1104 56-57 0.8746266039074975 1104 58-59 0.8989072245792933 1104 60-61 0.7929637673111642 1104 62-63 0.6905759135375646 1104 64-65 0.7987767329316711 1104 66-67 0.8258447471047923 1104 68-69 0.7441350929918897 1104 70-71 0.8610147586554326 1104 72-73 0.9232895753867112 1104 74-75 0.8479309176682897 1104 76-77 0.6353362411787344 1104 78-79 0.5772981983447067 1104 80-81 0.6778411513076605 1104 82-83 0.64564771807634 1104 84-85 0.3936431797493647 1104 86-87 0.10744148709281376 1104 88-89 -0.04852694346448416 1104 90-91 -0.6705269467466373 1104 92-93 -4.117518588132672 1104 94-95 -1.4857193266820126 1104 96-97 -0.41322236330798034 1104 98-99 -0.3831851936243318 1104 100-101 -0.3323714865795466 1105 1 0.9610180432288331 1105 2 0.9522475012711809 1105 3 0.9064229623576665 1105 4 0.7633891845298848 1105 5 0.6947587459901854 1105 6 0.7735223079550408 1105 7 0.8926511037814677 1105 8 0.8757905937014172 1105 9 1.0626594467918196 1105 10-11 0.9458118401273836 1105 12-13 0.7426328884385569 1105 14-15 0.9028518419786593 1105 16-17 0.8340508521169951 1105 18-19 0.9214807514766719 1105 20-21 0.8657647175805678 1105 22-23 0.7176130380507502 1105 24-25 0.9158065891844487 1105 26-27 0.9291552492266462 1105 28-29 0.9515771582182211 1105 30-31 0.8768915396376826 1105 32-33 0.9986621397366164 1105 34-35 0.9667595240969291 1105 36-37 1.0044661299497974 1105 38-39 1.0382801726581263 1105 40-41 1.0310257151308448 1105 42-43 1.0078286070288627 1105 44-45 0.9745793480617948 1105 46-47 0.9139341763067179 1105 48-49 0.9170789213902069 1105 50-51 0.7870850717127915 1105 52-53 0.6408434582933431 1105 54-55 1.00979432544462 1105 56-57 0.7983070751537404 1105 58-59 0.8213531055536727 1105 60-61 0.7735822402607084 1105 62-63 0.8931794101339854 1105 64-65 0.8712819575920676 1105 66-67 0.8548351947343669 1105 68-69 0.732822797221754 1105 70-71 0.7975429869217514 1105 72-73 0.973111770831288 1105 74-75 0.7292600803334146 1105 76-77 0.2882836442507113 1105 78-79 0.10993654614400583 1105 80-81 0.4462995291865113 1105 82-83 0.5192372862717463 1105 84-85 0.296118777867278 1105 86-87 -0.04241121070437259 1105 88-89 -0.29667981461167514 1105 90-91 -1.3787330611093154 1105 92-93 -7.258381850011979 1105 94-95 -2.7917752516885024 1105 96-97 -1.2122943065796434 1105 98-99 -0.5885112516935607 1105 100-101 -0.4059643768133583 1106 1 0.712069102308714 1106 2 0.8525046681907256 1106 3 0.7275348792621728 1106 4 0.704112961524892 1106 5 0.673354905363496 1106 6 0.6346296128991966 1106 7 0.6778331759552998 1106 8 0.6327528157305409 1106 9 0.8652809117910465 1106 10-11 0.6048927232175245 1106 12-13 0.6347334859882068 1106 14-15 0.6768118950496245 1106 16-17 0.682257200580672 1106 18-19 0.7306533724737889 1106 20-21 0.6899391599678282 1106 22-23 0.8339551686427527 1106 24-25 0.9147922921073715 1106 26-27 0.7341658266574456 1106 28-29 0.8483440236225093 1106 30-31 0.8257990397546493 1106 32-33 0.8434625721914486 1106 34-35 0.8964455097530575 1106 36-37 0.8568353574798593 1106 38-39 0.7644583948476651 1106 40-41 0.6068740818953202 1106 42-43 0.6169373958211324 1106 44-45 0.5873831358848847 1106 46-47 0.5133611903631774 1106 48-49 0.4561903503626752 1106 50-51 0.34243695091953086 1106 52-53 0.4045824213375049 1106 54-55 0.800889231567826 1106 56-57 0.7133881019498993 1106 58-59 0.7770110284336802 1106 60-61 0.6883754372849928 1106 62-63 0.6277656872125412 1106 64-65 0.5737056597262686 1106 66-67 0.31994680083284877 1106 68-69 0.41685171220794714 1106 70-71 0.23447025162822754 1106 72-73 0.43464660115793663 1106 74-75 0.2853944053496207 1106 76-77 0.2141823081190104 1106 78-79 0.29251628627006454 1106 80-81 0.1835318349661641 1106 82-83 0.24897264998163138 1106 84-85 0.08146419030353158 1106 86-87 -0.042486823460123446 1106 88-89 -0.062438599059341016 1106 90-91 -0.7268863553189675 1106 92-93 -4.0987541321302725 1106 94-95 -1.807071586851027 1106 96-97 -0.9587735093120635 1106 98-99 -0.8889349337757615 1106 100-101 -0.8626959172749551 1107 1 0.6914255667398059 1107 2 0.7234170911569286 1107 3 0.6014765906900088 1107 4 0.6288617356322419 1107 5 0.6313309369795874 1107 6 0.4590614410932048 1107 7 0.4544499845814869 1107 8 0.46194243701341975 1107 9 0.5258255909924472 1107 10-11 0.4488721525707078 1107 12-13 0.5046328754848588 1107 14-15 0.5377015142499602 1107 16-17 0.4746568263650275 1107 18-19 0.4973797384970595 1107 20-21 0.6302582925436511 1107 22-23 0.6145582309313937 1107 24-25 0.5916272062988881 1107 26-27 0.47962449713462263 1107 28-29 0.43282829166728476 1107 30-31 0.5340393932090208 1107 32-33 0.5308375199278075 1107 34-35 0.5265829651187133 1107 36-37 0.4237920839900582 1107 38-39 0.4314833644506564 1107 40-41 0.4717107069700148 1107 42-43 0.42031338642728855 1107 44-45 0.3972538344474472 1107 46-47 0.52799801215739 1107 48-49 0.5846415525464508 1107 50-51 0.3910935952496679 1107 52-53 0.4636303180565484 1107 54-55 0.5866227406885365 1107 56-57 0.37552572071450996 1107 58-59 0.3588168351839798 1107 60-61 0.2665710482609178 1107 62-63 0.30188008890804596 1107 64-65 0.408794263610357 1107 66-67 0.4666952817832559 1107 68-69 0.355309778749767 1107 70-71 0.37942334651656395 1107 72-73 0.27696841219505686 1107 74-75 0.0806665286869972 1107 76-77 0.08258207167111209 1107 78-79 0.0723173720721384 1107 80-81 -0.02890222474493953 1107 82-83 0.3183204879090944 1107 84-85 -0.00809085191587755 1107 86-87 -0.012463867508955673 1107 88-89 -0.2721114967796048 1107 90-91 -0.5401702097401113 1107 92-93 -3.9293853086508044 1107 94-95 -1.7371462457557065 1107 96-97 -1.0264086618738197 1107 98-99 -0.9163743440016496 1107 100-101 -0.7966691230503073 1108 1 0.26601507414869374 1108 2 0.32402294214505645 1108 3 0.13141306542387454 1108 4 -0.3477884815429704 1108 5 -0.7348878644250689 1108 6 -0.1316115538285274 1108 7 0.1750215575213545 1108 8 0.10740128515184466 1108 9 0.3732758646541683 1108 10-11 0.31777889024268546 1108 12-13 0.14337498585201303 1108 14-15 0.17221444491828208 1108 16-17 0.33095518329141527 1108 18-19 0.18447105248155538 1108 20-21 0.2283167340232879 1108 22-23 0.12790510117016396 1108 24-25 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58-59 0.5365173806169601 2311 60-61 0.4678500453437522 2311 62-63 0.36303230128951114 2311 64-65 0.4891395844075781 2311 66-67 0.40115785765435064 2311 68-69 0.5523757458723182 2311 70-71 0.7518561022640391 2311 72-73 0.6651735192948891 2311 74-75 0.713566280361281 2311 76-77 0.4857216313538295 2311 78-79 0.4962098664714638 2311 80-81 0.5298389824130894 2311 82-83 0.6717610468641624 2311 84-85 0.6510497591342137 2311 86-87 0.9567679900268189 2311 88-89 1.0087494752697488 2311 90-91 1.4718414915211362 2311 92-93 2.5254762398360846 2311 94-95 1.9343084858839568 2311 96-97 1.4894905083664334 2311 98-99 1.285107537004496 2311 100-101 1.587466143066866 2312 1 0.2488152807372188 2312 2 0.06363239509803265 2312 3 -0.04596235879312616 2312 4 -0.049035041584282624 2312 5 0.11711980318744253 2312 6 0.1879252722566349 2312 7 0.13218805534162925 2312 8 -0.6632082885148449 2312 9 0.06454788153575208 2312 10-11 0.08638198051362878 2312 12-13 0.08533289344031658 2312 14-15 -0.09134292694564294 2312 16-17 0.1562862673769132 2312 18-19 0.20128482943012926 2312 20-21 0.2848439839969288 2312 22-23 0.04651961340274369 2312 24-25 0.2755956781798332 2312 26-27 0.1477093584410767 2312 28-29 0.004960506393722142 2312 30-31 0.2974302178910264 2312 32-33 0.35575524144514503 2312 34-35 0.263345309106235 2312 36-37 0.33946383838438265 2312 38-39 0.34675613758928847 2312 40-41 0.5306149730985865 2312 42-43 0.5777050419076275 2312 44-45 0.42727789059357946 2312 46-47 0.4592378535760204 2312 48-49 0.3616635418602172 2312 50-51 0.5106202095927266 2312 52-53 0.5668772723389246 2312 54-55 0.5968917520411345 2312 56-57 0.41226522295998436 2312 58-59 0.3697137664161332 2312 60-61 0.5450869370183753 2312 62-63 0.5782034049713012 2312 64-65 0.7607260912389862 2312 66-67 0.6814232930558433 2312 68-69 0.4274290285399225 2312 70-71 0.6205653632560519 2312 72-73 0.7710771842318636 2312 74-75 0.8523813636752813 2312 76-77 0.9461867721967181 2312 78-79 0.9643634944095396 2312 80-81 0.9003728750459814 2312 82-83 0.9499022778938055 2312 84-85 1.0982636477509438 2312 86-87 0.949326445559727 2312 88-89 1.1956220342983208 2312 90-91 1.519383410760696 2312 92-93 2.5047266244850483 2312 94-95 1.8475314016256483 2312 96-97 1.5581954958183708 2312 98-99 1.6152194466929366 2312 100-101 1.5575930052892737 2313 1 -0.1743387893859989 2313 2 -1.0000783718585993 2313 3 -0.3252742249585481 2313 4 -0.10223610210703526 2313 5 -0.8022815423463214 2313 6 -0.36110599477671457 2313 7 -1.1972329735039438 2313 8 -1.1309792982263502 2313 9 -1.1429742838315278 2313 10-11 -2.0824521787785173 2313 12-13 -0.6397103447719346 2313 14-15 -0.2820258011509509 2313 16-17 -1.7028636666836903 2313 18-19 -1.225616054915939 2313 20-21 -1.4360177593940548 2313 22-23 -1.281914921526031 2313 24-25 -0.4198289219599829 2313 26-27 -0.8747823327177855 2313 28-29 -2.3577783336102627 2313 30-31 -0.766523769606458 2313 32-33 -0.35089738362328404 2313 34-35 -0.20817788788867375 2313 36-37 -0.20271200039514525 2313 38-39 -0.43294957391437094 2313 40-41 -0.37777410573793446 2313 42-43 -0.15407822315798825 2313 44-45 -0.8204424093664855 2313 46-47 -1.080751507764088 2313 48-49 -0.5481369802818641 2313 50-51 -0.3566581501671173 2313 52-53 -0.17918848317607328 2313 54-55 -0.17171847002746077 2313 56-57 -0.5436025472139185 2313 58-59 -0.15707392545436605 2313 60-61 -0.2940544956664297 2313 62-63 -0.26970650432546606 2313 64-65 -0.3083948103844527 2313 66-67 -0.196349728568336 2313 68-69 -0.2647118554957011 2313 70-71 -0.2576836518320995 2313 72-73 -0.22891660689816717 2313 74-75 -0.11127435926105989 2313 76-77 -0.010332809922452668 2313 78-79 0.0748537384240997 2313 80-81 0.026263743410176232 2313 82-83 -0.055826718066249725 2313 84-85 -0.04858949993717587 2313 86-87 -0.029631992622913828 2313 88-89 0.24224837913060426 2313 90-91 0.8055769734759579 2313 92-93 2.129019007335909 2313 94-95 1.4011354416895792 2313 96-97 0.8828133070364892 2313 98-99 0.7011965944694154 2313 100-101 0.8226844100043103 2314 1 -0.554165824421208 2314 2 -0.6000567026589536 2314 3 -1.797556904378638 2314 4 -0.5840389795176648 2314 5 -0.4031805785968885 2314 6 -2.9945061218477242 2314 7 -3.9958361344984397 2314 8 -1.6006478949042489 2314 9 -1.9782486789046132 2314 10-11 -1.2190098850937332 2314 12-13 -2.1686251241671144 2314 14-15 -2.7944398383171674 2314 16-17 -1.9599893886713957 2314 18-19 -2.3089301234671566 2314 20-21 -2.1239380049626178 2314 22-23 -3.1388455618332145 2314 24-25 -1.7427593514635475 2314 26-27 -1.9588519968393143 2314 28-29 -1.3210161242233767 2314 30-31 -1.6471854221302422 2314 32-33 -2.469564869165872 2314 34-35 -0.8114376892268709 2314 36-37 -0.48980298689134116 2314 38-39 -0.65023953430331 2314 40-41 -0.8911312241583502 2314 42-43 -0.6394631134841617 2314 44-45 -1.4401699191809243 2314 46-47 -1.625416203397215 2314 48-49 -1.8827743126620646 2314 50-51 -1.0717787174761817 2314 52-53 -0.6492898161262701 2314 54-55 -0.46964181876673194 2314 56-57 -0.47453903938268027 2314 58-59 -0.4422591411718386 2314 60-61 -0.3547705331706297 2314 62-63 -0.43078691413242964 2314 64-65 -0.3616862617085417 2314 66-67 -0.3168696362622576 2314 68-69 -0.2080602061339718 2314 70-71 -0.41068421770440366 2314 72-73 -0.18513652101041345 2314 74-75 0.2039536119071279 2314 76-77 0.066913058355901 2314 78-79 0.15848171595172644 2314 80-81 0.23907406784166696 2314 82-83 0.3229513039480061 2314 84-85 0.22535387632433057 2314 86-87 0.3777006172260897 2314 88-89 0.5601289429773111 2314 90-91 1.241706242028588 2314 92-93 2.1071863465525063 2314 94-95 1.4748257358312955 2314 96-97 1.2397740369116583 2314 98-99 1.2437384740251751 2314 100-101 1.3997480332475902 2315 1 -7.226514488431754 2315 2 -3.092676517595315 2315 3 -1.374853791092157 2315 4 -0.8872623304434981 2315 5 -1.1924770327977257 2315 6 -1.5663565914354223 2315 7 -3.046815825881744 2315 8 -2.8187384493894747 2315 9 -3.341561198828714 2315 10-11 -2.1213662393850754 2315 12-13 -2.8084700751355953 2315 14-15 -4.632577274004525 2315 16-17 -4.49435886671327 2315 18-19 -3.1896793315478718 2315 20-21 -4.2442941653931 2315 22-23 -5.5123886747044395 2315 24-25 -3.5307680073160768 2315 26-27 -4.347423117472903 2315 28-29 -2.811281264529299 2315 30-31 -2.734453312530974 2315 32-33 -1.7349414357266895 2315 34-35 -2.5175917451588745 2315 36-37 -3.0075132031605136 2315 38-39 -2.235803676995637 2315 40-41 -3.511050614432108 2315 42-43 -7.406092485457783 2315 44-45 -4.100019223270586 2315 46-47 -2.9127122864396497 2315 48-49 -5.613827358569651 2315 50-51 -6.540757246860743 2315 52-53 -6.674452594514026 2315 54-55 -4.547617976680055 2315 56-57 -3.932956759152024 2315 58-59 -4.094485350903863 2315 60-61 -2.9852929578684595 2315 62-63 -3.2508874161760737 2315 64-65 -2.0151907633747257 2315 66-67 -2.566066577553091 2315 68-69 -2.125688527269741 2315 70-71 -1.7665137899775871 2315 72-73 -2.276021631154755 2315 74-75 -2.8028582878838364 2315 76-77 -2.738273279608901 2315 78-79 -1.8897928465520124 2315 80-81 -1.6095045599430264 2315 82-83 -1.9865440640954404 2315 84-85 -1.8676480731321057 2315 86-87 -2.1746346421572937 2315 88-89 -1.6829776528527667 2315 90-91 -0.9133008562883802 2315 92-93 -0.08746150728993385 2315 94-95 -0.21035244644667372 2315 96-97 -0.32948176482956626 2315 98-99 -0.5058762245940862 2315 100-101 -0.6653787187466271 2316 1 -3.7218208211901285 2316 2 -2.7467001815809127 2316 3 -4.290534058748197 2316 4 -4.2618528693568685 2316 5 -3.7511583031568954 2316 6 -4.195393718304942 2316 7 -2.633622655196355 2316 8 -1.3637242624109973 2316 9 -1.5980076784104469 2316 10-11 -2.7809373188566084 2316 12-13 -3.670592281197564 2316 14-15 -2.595923283787954 2316 16-17 -2.3550790986345262 2316 18-19 -2.623741770557288 2316 20-21 -5.323837271317583 2316 22-23 -6.181951959369989 2316 24-25 -3.9285321495781176 2316 26-27 -3.6944831976603822 2316 28-29 -4.143629877216874 2316 30-31 -5.82330774698675 2316 32-33 -4.225784696588228 2316 34-35 -5.763517934028631 2316 36-37 -8.687901401761913 2316 38-39 -5.584985989619383 2316 40-41 -4.9077570271765545 2316 42-43 -4.164361429521719 2316 44-45 -3.053471511912896 2316 46-47 -5.134610524300399 2316 48-49 -2.8023204410689537 2316 50-51 -3.974798826192494 2316 52-53 -5.102282365637809 2316 54-55 -6.582981424194944 2316 56-57 -4.715762858138145 2316 58-59 -5.2032163008631755 2316 60-61 -5.828242422781763 2316 62-63 -5.727480759876908 2316 64-65 -4.647285151737812 2316 66-67 -5.940087791125979 2316 68-69 -4.714375065296931 2316 70-71 -5.5215244883548 2316 72-73 -7.543919354476792 2316 74-75 -7.349496990594108 2316 76-77 -6.706265183176768 2316 78-79 -5.591375736437371 2316 80-81 -5.490247025403477 2316 82-83 -4.565823546852158 2316 84-85 -5.943503271963195 2316 86-87 -4.547547415616879 2316 88-89 -4.384842927627471 2316 90-91 -4.967691446799481 2316 92-93 -3.7238519702453026 2316 94-95 -3.64292809394785 2316 96-97 -4.603613654577298 2316 98-99 -5.438470633816845 2316 100-101 -4.740395274617239 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12272.0 3 4827.0 4 5023.0 5 5569.0 6 6529.0 7 7708.0 8 9575.0 9 12017.0 10 14752.0 11 17594.0 12 21370.0 13 24554.0 14 26620.0 15 29828.0 16 33868.0 17 38625.0 18 34924.0 19 32301.0 20 33940.0 21 36823.0 22 40898.0 23 45407.0 24 51303.0 25 58539.0 26 65438.0 27 74947.0 28 85271.0 29 96751.0 30 111732.0 31 128516.0 32 148519.0 33 172290.0 34 205344.0 35 248415.0 36 289093.0 37 259635.0 38 84578.0 39 1488.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.16514064717003 18.817601278352868 13.531150723443163 22.486107351033944 2 15.01045543591379 21.20971510636653 40.376706461937204 23.403122995782475 3 19.467642236378065 26.30751965829164 30.29208435964059 23.932753745689702 4 12.33884889515253 16.700242241361895 38.38127143307752 32.57963743040805 5 13.178760099440645 38.41621348663766 34.16089185829708 14.24413455562461 6 30.132510388116902 36.854813371202695 18.268948822382328 14.743727418298077 7 27.150587441070417 33.98259292879974 21.533511156272493 17.333308473857347 8 25.45505109335388 34.35134220797708 20.6186123597404 19.574994338928633 9 26.876461312598554 16.86379983998384 20.709197821916 35.5505410255016 10-11 24.501276825324197 26.44427695020041 28.012318271604407 21.042127952870977 12-13 26.763214715605265 24.110638929251568 27.960442372385636 21.165703982757528 14-15 23.471890998170263 25.10492187449928 27.150764901780615 24.27242222554984 16-17 21.76135846104576 28.504081275540138 27.669142938934005 22.065417324480098 18-19 21.589056952633992 27.695675142362163 29.789855579120733 20.925412325883112 20-21 22.84726997261232 26.571547889594623 29.05920400909038 21.521978128702678 22-23 22.338924596331715 26.53121602345677 28.879515401857823 22.250343978353694 24-25 22.540004216338993 26.558894192235915 29.196068506765087 21.705033084660005 26-27 22.637870322404385 26.20877841844212 29.56334085575259 21.590010403400907 28-29 21.834199698119544 26.813070397838644 29.741326377543327 21.61140352649848 30-31 21.922028006338493 27.003450028029658 29.355143028971103 21.71937893666075 32-33 21.84912860009999 26.935256249454724 29.133589909548043 22.08202524089724 34-35 22.547857453036308 26.49845194922277 28.896352001585452 22.05733859615547 36-37 22.656166510252422 26.252858688957154 29.34243839124547 21.74853640954496 38-39 22.7671656800641 26.241199652765633 29.53069736753141 21.460937299638854 40-41 22.255349225228347 26.55784880072521 29.559212262059674 21.627589711986765 42-43 21.786271044713022 27.051937232666866 28.973549562319707 22.18824216030041 44-45 22.13096760757973 27.086710295856886 28.44652212360499 22.335799972958398 46-47 22.292021386251424 27.038254436702868 27.9344065319116 22.735317645134106 48-49 22.62336695204155 26.961668920308153 27.95233188734928 22.462632240301016 50-51 22.597847996142463 27.320669098553207 28.146498586755047 21.93498431854928 52-53 22.44363041799908 27.411427003130928 28.053998642511775 22.090943936358215 54-55 21.734956666413993 26.953373877787428 28.47676468473091 22.834904771067663 56-57 22.346766338230058 27.780864015818118 27.50024628373869 22.372123362213138 58-59 22.486169929804852 27.07503511311679 27.775160255507302 22.663634701571052 60-61 22.42135372942858 26.79775463175685 28.331075440308567 22.449816198506 62-63 22.98157002899402 26.735911166855892 28.03382299114793 22.248695813002158 64-65 22.44950486317813 27.005163782002654 28.235784545017832 22.309546809801375 66-67 22.01268235252977 27.247649743263956 28.0016163782636 22.738051525942677 68-69 21.86758730875378 27.62837397050407 28.026340175722915 22.477698545019233 70-71 22.10626486422109 27.433788219056236 27.534273802231084 22.925673114491588 72-73 22.234463485449762 27.048752142598946 27.498435562672718 23.21834880927857 74-75 22.13790578016616 27.62133170997943 27.4411145475212 22.799647962333214 76-77 22.521069591561513 26.90901443022592 27.685068162495252 22.88484781571732 78-79 22.10779326749824 27.10867377264774 27.96824505799477 22.815287901859246 80-81 22.006255333459034 27.697714606728468 27.5627331414098 22.733296918402694 82-83 22.127665364620032 27.482313515593944 27.353378825592166 23.036642294193857 84-85 22.174387225989555 27.081612640306524 27.277348962181613 23.466651171522308 86-87 22.391130286069654 27.0165772699005 27.62334810323383 22.968944340796018 88-89 22.51679920071376 26.631043484514798 27.608400280683203 23.243757034088237 90-91 21.818294653753348 26.932391843700167 28.1060568772989 23.14325662524758 92-93 21.500455261488625 27.355714877570943 28.156577182621884 22.98725267831855 94-95 22.067052571424128 27.292394773569285 27.194836547694486 23.445716107312105 96-97 21.996145713267584 26.931464032132922 27.080884226954616 23.991506027644874 98-99 22.040282038827588 26.929664127083143 26.90615570624624 24.12389812784303 100-101 22.42410312085377 26.90863983481871 27.136565281554336 23.530691762773188 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3375.0 1 2109.0 2 924.0 3 983.0 4 861.5 5 879.5 6 1241.5 7 2241.5 8 3510.5 9 3995.0 10 3562.5 11 3097.5 12 3139.0 13 3244.5 14 3492.0 15 3716.5 16 3848.0 17 4147.5 18 4431.5 19 4798.5 20 5320.5 21 6083.0 22 7142.5 23 8451.5 24 10102.0 25 12222.0 26 15228.0 27 19166.0 28 22890.0 29 26552.0 30 30564.5 31 34643.0 32 38049.0 33 41637.5 34 46373.5 35 50550.5 36 55155.5 37 60442.0 38 63638.5 39 64865.0 40 67556.5 41 71895.0 42 75579.5 43 79505.5 44 84402.5 45 88036.5 46 92455.5 47 104456.0 48 133966.5 49 137189.5 50 111569.0 51 99431.5 52 91855.0 53 87176.0 54 83201.0 55 76945.0 56 70117.0 57 62832.5 58 55334.0 59 48201.0 60 40905.5 61 33445.5 62 26771.0 63 20967.0 64 15950.0 65 11927.5 66 8715.0 67 6530.0 68 4786.0 69 3246.5 70 2203.5 71 1521.0 72 1024.5 73 714.5 74 503.5 75 335.5 76 207.5 77 138.0 78 89.0 79 55.0 80 30.5 81 27.5 82 24.5 83 13.5 84 8.5 85 7.0 86 8.0 87 4.5 88 3.5 89 3.5 90 4.0 91 4.0 92 3.0 93 4.0 94 3.5 95 3.0 96 1.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09003125093378318 2 0.10182844933200304 3 0.09794779196416756 4 0.10054783240061735 5 0.09635672244335501 6 0.08017438121948106 7 0.09360145571219182 8 0.0885566011340057 9 0.08316248739271437 10-11 0.09055513967844096 12-13 0.08964318519699964 14-15 0.09575522055134052 16-17 0.09241785521500201 18-19 0.09558059096978792 20-21 0.10605836586294372 22-23 0.11473163508005603 24-25 0.13817080558178235 26-27 0.14304103057841586 28-29 0.14276938456266738 30-31 0.14418582450192735 32-33 0.14119771832869402 34-35 0.13526031255590573 36-37 0.13923798635793708 38-39 0.13213638337479816 40-41 0.12751840110707394 42-43 0.1286437917437462 44-45 0.11917498776622763 46-47 0.1376663201239637 48-49 0.15561436045020283 50-51 0.16521898743559565 52-53 0.17404748294742137 54-55 0.1706713110374045 56-57 0.14092607231294552 58-59 0.1325050458247425 60-61 0.12877961475162047 62-63 0.1260825578809748 64-65 0.11244204723303308 66-67 0.12526761983372936 68-69 0.12309445170774148 70-71 0.1311468157460001 72-73 0.15805917459193916 74-75 0.1719519279687902 76-77 0.17750126800479493 78-79 0.17447435525788327 80-81 0.18153715166734385 82-83 0.1804699708911891 84-85 0.15654571821848334 86-87 0.1584472403287227 88-89 0.1780445600362919 90-91 0.2627787136629796 92-93 0.5247036827050355 94-95 0.17746246143111657 96-97 0.19176268383159034 98-99 0.21203911857853075 100-101 0.21916012484850886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2576883.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.19732487448369 #Duplication Level Percentage of deduplicated Percentage of total 1 71.85183909204787 34.63066431548558 2 13.777107259036233 13.280394287887537 3 5.655813961150052 8.177853087455688 4 2.7925037584684094 5.383648434404746 5 1.6585723962668917 3.996937630536314 6 0.9814576146562557 2.8382178902474036 7 0.6683899311254397 2.2550224657300615 8 0.469630465339076 1.8107945687121918 9 0.3576172290149981 1.551257439079365 >10 1.6465047417182825 13.63877794688659 >50 0.08788390427659493 2.898282281929408 >100 0.04626750010014528 4.026787901244953 >500 0.00345269443065952 1.1323055075704695 >1k 0.0024662103076110416 2.3279659212416317 >5k 3.2882804101480557E-4 1.1360695576506052 >10k+ 1.6441402050740278E-4 0.9150207639376094 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11749 0.45593843414699076 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11345 0.44026057838093535 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8807 0.3417694943852709 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8312 0.3225602404144853 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6013 0.2333439275279475 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5541 0.21502722475176406 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4399 0.17071011761108285 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3743 0.14525300527808208 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3565 0.13834543516333492 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3484 0.13520210269538818 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3015 0.11700181964023977 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2953 0.11459581207218178 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2903 0.11265548338826403 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.940328683917741E-5 0.0 18-19 0.0 0.0 0.0 1.3582300787424187E-4 0.0 20-21 3.880657367835482E-5 0.0 0.0 2.5224272890930636E-4 0.0 22-23 3.880657367835482E-5 0.0 0.0 7.373248998887416E-4 0.0 24-25 3.880657367835482E-5 0.0 0.0 0.0014746497997774831 0.0 26-27 3.880657367835482E-5 0.0 0.0 0.002464217428575531 0.0 28-29 3.880657367835482E-5 0.0 0.0 0.005995615633305819 0.0 30-31 3.880657367835482E-5 0.0 0.0 0.01618234122387396 0.0 32-33 3.880657367835482E-5 0.0 0.0 0.03599309708667409 0.0 34-35 3.880657367835482E-5 0.0 0.0 0.06591296539268565 0.0 36-37 3.880657367835482E-5 0.0 0.0 0.10877482602042855 0.0 38-39 3.880657367835482E-5 0.0 0.0 0.16667423394853395 0.0 40-41 7.761314735670964E-5 0.0 0.0 0.23223794017811442 0.0 42-43 7.761314735670964E-5 0.0 0.0 0.30338979301737795 0.0 44-45 7.761314735670964E-5 0.0 0.0 0.385310470052385 0.0 46-47 7.761314735670964E-5 0.0 0.0 0.48611054518191166 0.0 48-49 7.761314735670964E-5 0.0 0.0 0.5929450425184224 0.0 50-51 7.761314735670964E-5 0.0 0.0 0.7041452793937482 0.0 52-53 7.761314735670964E-5 0.0 0.0 0.8106111142803146 0.0 54-55 7.761314735670964E-5 0.0 0.0 0.9278845799363029 0.0 56-57 7.761314735670964E-5 0.0 0.0 1.064813575160378 0.0 58-59 7.761314735670964E-5 0.0 0.0 1.226481761104404 0.0 60-61 7.761314735670964E-5 0.0 0.0 1.421698229993368 0.0 62-63 7.761314735670964E-5 0.0 0.0 1.6037592704053696 0.0 64-65 7.761314735670964E-5 0.0 0.0 1.7857426976700146 0.0 66-67 7.761314735670964E-5 0.0 0.0 1.9893607897603423 0.0 68-69 7.761314735670964E-5 0.0 0.0 2.226139099058824 0.0 70-71 7.761314735670964E-5 0.0 0.0 2.4930507128185484 0.0 72-73 7.761314735670964E-5 0.0 0.0 2.772807302465808 0.0 74-75 7.761314735670964E-5 0.0 0.0 3.0545818339443427 0.0 76-77 7.761314735670964E-5 0.0 0.0 3.3516849620258276 0.0 78-79 7.761314735670964E-5 0.0 0.0 3.6904081403773477 0.0 80-81 7.761314735670964E-5 0.0 0.0 4.054258575185602 0.0 82-83 7.761314735670964E-5 0.0 0.0 4.424221045348197 0.0 84-85 7.761314735670964E-5 0.0 0.0 4.816788344678435 0.0 86-87 7.761314735670964E-5 0.0 0.0 5.241681519882742 0.0 88-89 7.761314735670964E-5 0.0 0.0 5.679322654540389 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12145 0.0 26.335125 1 TACATGG 12215 0.0 26.185226 2 ACATGGG 12575 0.0 23.699629 3 CATGGGG 4475 0.0 21.739136 4 GTATAGG 510 0.0 21.400835 1 GAGTACT 8970 0.0 21.160803 12-13 CATGGGA 8435 0.0 20.703522 4 AGTACTT 9500 0.0 20.354883 12-13 GTACTTT 10390 0.0 18.383297 14-15 ATGGGAG 3525 0.0 17.500774 5 GCTTATA 2370 0.0 17.219603 1 TACTTTT 12045 0.0 16.152884 14-15 ACTTTTT 12650 0.0 16.07544 16-17 ATGGGGA 2510 0.0 15.691969 5 ATGGGAT 3515 0.0 15.660503 5 ATAGGAG 1595 0.0 15.470904 5 ATTCGCG 200 7.747076E-9 15.4347 80-81 TTATAGG 1605 0.0 15.37511 3 AGTACAT 9165 0.0 15.326717 2 CTTATAG 1645 0.0 15.001247 2 >>END_MODULE