FastQCFastQC Report
Fri 27 May 2016
SRR939605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576883
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120160.46629978931911154No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA113870.4418904544754263No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA93780.36392804795561146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91280.3542264045360228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66540.25821894125577294No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT64590.25065165938849376No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61600.23904849385866567No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA45500.17656991023651442No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG44770.17373703035799454No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT41690.16178460566506125No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC36300.140867862452428No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34360.13333938715882715No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG29540.11463461864586012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG117800.025.5539881
TACATGG118000.024.787732
ACATGGG116750.023.6809943
GAGTACT90400.021.71945612-13
CATGGGA83150.021.5814914
AGTACTT94450.020.11593812-13
GTACTTT101500.019.43689214-15
CATGGGG39100.019.273564
ATGGGAG33300.018.8116175
ATGGGAT35750.018.7081575
ACTTTTT118200.016.96924216-17
TACTTTT115950.016.48734314-15
AGTACAT90150.016.1965712
GTATCAA315200.014.990561
GCTTATA24350.014.8968651
CGCGTAG1502.0815312E-514.57226766-67
GTGTAGC12350.014.1136031
GTGTAGG11350.014.1119221
AACGCAG336000.013.996576
ATCAACG336050.013.9736543
Produced by FastQC (version 0.11.5)