##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939605_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2576883 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.600102526967657 31.0 31.0 34.0 30.0 34.0 2 31.804262746892274 31.0 31.0 34.0 30.0 34.0 3 31.850432867926095 31.0 31.0 34.0 30.0 34.0 4 35.6183513182399 37.0 35.0 37.0 33.0 37.0 5 35.35520859891582 37.0 35.0 37.0 33.0 37.0 6 35.38639123312933 37.0 35.0 37.0 33.0 37.0 7 35.31972231568139 37.0 35.0 37.0 32.0 37.0 8 35.29256120669817 37.0 35.0 37.0 32.0 37.0 9 36.90352064878382 39.0 37.0 39.0 33.0 39.0 10-11 36.79107743735358 39.0 37.0 39.0 32.0 39.0 12-13 36.73502328200388 39.0 35.0 39.0 32.0 39.0 14-15 37.77350523869342 40.0 37.0 41.0 32.0 41.0 16-17 37.743379695546906 39.0 37.0 41.0 32.0 41.0 18-19 37.759584156517775 39.5 37.0 41.0 32.0 41.0 20-21 37.70719043123029 39.5 37.0 41.0 32.0 41.0 22-23 37.57674504430352 39.0 36.0 41.0 32.0 41.0 24-25 37.44011388953243 39.0 36.0 41.0 32.0 41.0 26-27 37.159219491144924 39.0 36.0 40.5 31.0 41.0 28-29 36.95581813376859 39.0 36.0 40.0 31.0 41.0 30-31 36.73153980991764 38.5 35.5 40.0 30.0 41.0 32-33 36.56698790748358 38.0 35.0 40.0 30.0 41.0 34-35 36.54504667072584 38.0 35.0 40.0 30.0 41.0 36-37 36.41741573055509 38.5 35.0 40.0 30.0 41.0 38-39 36.43638205537465 39.0 35.0 40.0 30.0 41.0 40-41 36.32955260289272 39.0 35.0 40.0 30.0 41.0 42-43 36.15909181751752 38.5 35.0 40.0 30.0 41.0 44-45 35.94114963698391 38.0 35.0 40.0 29.0 41.0 46-47 35.61311844581225 38.0 35.0 40.0 27.5 41.0 48-49 35.30609868589299 38.0 34.0 40.0 27.0 41.0 50-51 35.052127318159194 38.0 34.0 40.0 26.0 41.0 52-53 34.78726799004844 38.0 34.0 40.0 26.0 41.0 54-55 34.514970217895026 38.0 33.5 40.0 25.5 41.0 56-57 32.56506445965921 37.0 30.5 39.5 16.0 41.0 58-59 27.6323015053458 33.0 18.0 38.5 6.5 40.0 60-61 28.34875603587745 33.0 20.0 38.0 8.5 40.0 62-63 31.371397537257224 35.0 29.5 38.5 12.0 40.0 64-65 31.57765661071923 35.5 31.0 39.0 5.0 40.0 66-67 24.948851965727584 28.0 18.0 35.5 2.0 39.0 68-69 17.499392871154804 13.0 7.5 28.0 2.0 35.5 70-71 16.37143498560082 12.5 7.5 27.0 2.0 34.0 72-73 17.51013453074897 14.0 7.0 29.0 2.0 34.0 74-75 18.50568244658372 17.5 8.5 30.0 2.0 34.5 76-77 19.663338420875142 21.5 8.0 30.0 2.0 34.5 78-79 22.755178252175206 27.5 10.5 33.5 2.0 35.5 80-81 26.372866948169552 31.5 23.5 34.5 2.0 36.0 82-83 27.661232000055882 33.5 26.5 35.0 2.0 36.0 84-85 27.721224052469594 34.0 27.5 35.0 2.0 36.0 86-87 27.48069237136494 34.0 27.0 35.0 2.0 36.0 88-89 27.056928467454675 33.5 26.0 35.0 2.0 35.0 90-91 25.766130243398713 33.0 21.0 35.0 2.0 35.0 92-93 23.405651905810238 31.5 2.0 34.5 2.0 35.0 94-95 16.09212234315644 7.5 2.0 32.0 2.0 35.0 96-97 13.514382298303804 2.0 2.0 30.0 2.0 34.0 98-99 13.220826479122259 2.0 2.0 30.5 2.0 34.0 100-101 12.93194724013469 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36527460627065267 1101 2 0.3986375778587252 1101 3 0.48120161260675687 1101 4 0.2413095722750711 1101 5 0.438928475906458 1101 6 0.5101944174244721 1101 7 0.5466115871720802 1101 8 0.5651956782378136 1101 9 0.605151695049976 1101 10-11 0.6061219063195509 1101 12-13 0.635329363260837 1101 14-15 0.819581237061719 1101 16-17 0.813833402712774 1101 18-19 0.5216865095398049 1101 20-21 0.5424425668175274 1101 22-23 0.6623674997821567 1101 24-25 0.5381884700414616 1101 26-27 0.5750885770481062 1101 28-29 0.5769518404288547 1101 30-31 0.7630678546884582 1101 32-33 0.6345797373257795 1101 34-35 0.667846243875637 1101 36-37 0.521255551203545 1101 38-39 0.6334242732181323 1101 40-41 0.8016576982145409 1101 42-43 0.80433304269053 1101 44-45 0.5576589711491948 1101 46-47 0.5790643488798892 1101 48-49 0.46657998990473715 1101 50-51 0.46799928461350504 1101 52-53 0.41851663408068873 1101 54-55 0.18082914887630608 1101 56-57 -2.0615569501536797 1101 58-59 -9.888393424235247 1101 60-61 -10.084367135832462 1101 62-63 -3.4792669490829766 1101 64-65 -4.828908193840402 1101 66-67 -9.654540930544176 1101 68-69 -4.614608173386912 1101 70-71 -4.494661556796924 1101 72-73 -4.5362005568035215 1101 74-75 -6.711838304724983 1101 76-77 -7.638970657822851 1101 78-79 -10.922036985717977 1101 80-81 -7.86529495112331 1101 82-83 -3.7944526771722877 1101 84-85 -2.897844365811636 1101 86-87 -2.6791124968566358 1101 88-89 -2.5995332503147672 1101 90-91 -9.524670634099639 1101 92-93 -17.26952584101375 1101 94-95 -11.24521917520409 1101 96-97 -9.553054716284674 1101 98-99 -9.654517247316937 1101 100-101 -9.42002172243105 1102 1 0.3532088223207772 1102 2 0.37735843441285155 1102 3 0.32575730936595804 1102 4 0.22547833145618057 1102 5 0.29484861087274794 1102 6 0.30938359332184007 1102 7 0.3988813743521362 1102 8 0.34405117321756506 1102 9 0.5029936313914405 1102 10-11 0.4845759806465182 1102 12-13 0.44793444930042625 1102 14-15 0.4932749627970381 1102 16-17 0.3726509366414419 1102 18-19 0.4516107371150895 1102 20-21 0.6073640895075627 1102 22-23 0.5734104522196546 1102 24-25 0.3227473884294767 1102 26-27 0.45405012918895693 1102 28-29 0.6106449460719006 1102 30-31 0.49453315411106047 1102 32-33 0.4330509960489479 1102 34-35 0.344055798000781 1102 36-37 0.2554018587678897 1102 38-39 0.2007044629806174 1102 40-41 0.2006833336843883 1102 42-43 0.3329210783308625 1102 44-45 0.31940098853030463 1102 46-47 0.34782570387415745 1102 48-49 0.4612338323029448 1102 50-51 0.5477695226077657 1102 52-53 0.3399261750912359 1102 54-55 0.272725754548226 1102 56-57 -2.5654656697201546 1102 58-59 -10.528146363793358 1102 60-61 -10.622452027177328 1102 62-63 -4.470109362254021 1102 64-65 -5.561248749942006 1102 66-67 -6.737398444031182 1102 68-69 -4.284059078115794 1102 70-71 -4.225165178634718 1102 72-73 -4.300263465520949 1102 74-75 -5.281075170707362 1102 76-77 -7.558327301398801 1102 78-79 -10.0980134706786 1102 80-81 -5.752796670632055 1102 82-83 -3.2109914646566864 1102 84-85 -2.8165146313893175 1102 86-87 -2.7957130084684003 1102 88-89 -2.9684421801424143 1102 90-91 -8.652632732919539 1102 92-93 -13.39882274190661 1102 94-95 -10.40155209918149 1102 96-97 -9.4202639161732 1102 98-99 -9.357150216126296 1102 100-101 -9.195348928017298 1103 1 0.36196245211736056 1103 2 0.3319786955612365 1103 3 0.2575642806708167 1103 4 0.2816677292022547 1103 5 0.2783758616399865 1103 6 0.26656739503111737 1103 7 0.1723694951893151 1103 8 0.17367798256298528 1103 9 0.36251290781671486 1103 10-11 0.3826696904798794 1103 12-13 0.25796308116099453 1103 14-15 0.4711258172896109 1103 16-17 0.47748599960680593 1103 18-19 0.4843699524713543 1103 20-21 0.38586425274101543 1103 22-23 0.4678532016996755 1103 24-25 0.4810830022815651 1103 26-27 0.47199030318450497 1103 28-29 0.3761421711031545 1103 30-31 0.44048454532529036 1103 32-33 0.32339530758304136 1103 34-35 0.44586926092625845 1103 36-37 0.2651986949305254 1103 38-39 0.41156405580639444 1103 40-41 0.49208093056942204 1103 42-43 0.32863593819106995 1103 44-45 0.3395551865218067 1103 46-47 0.48674837563913087 1103 48-49 0.3116833843643647 1103 50-51 0.3970585246122482 1103 52-53 0.4860868571598189 1103 54-55 0.40064881731914426 1103 56-57 -2.5064763676930006 1103 58-59 -9.905725986726168 1103 60-61 -10.229751621964414 1103 62-63 -4.156307711647376 1103 64-65 -5.319566950828538 1103 66-67 -6.461990287052146 1103 68-69 -4.071504602355018 1103 70-71 -4.099220823851098 1103 72-73 -4.357925212273985 1103 74-75 -4.977831623457877 1103 76-77 -6.68870850495113 1103 78-79 -9.206067732077598 1103 80-81 -4.825178869091246 1103 82-83 -2.5005410031816666 1103 84-85 -1.9825691551352769 1103 86-87 -1.809649248097628 1103 88-89 -1.9866124315889913 1103 90-91 -5.045319622653768 1103 92-93 -10.55230776570852 1103 94-95 -8.683816166070747 1103 96-97 -8.163742750095418 1103 98-99 -8.573254128993575 1103 100-101 -8.47263741287137 1104 1 0.2434362488557369 1104 2 0.23061295800506443 1104 3 0.20516722970768697 1104 4 0.278477675398193 1104 5 0.33928608771928026 1104 6 0.2084702861066532 1104 7 0.19756967230593858 1104 8 0.2529094046756484 1104 9 0.2622424967422674 1104 10-11 0.2718126520111426 1104 12-13 0.3260872011221778 1104 14-15 0.5368451806621053 1104 16-17 0.32646859391426375 1104 18-19 0.3642294018611807 1104 20-21 0.24847926390101804 1104 22-23 0.4091957246492868 1104 24-25 0.5163424412969917 1104 26-27 0.470193501658116 1104 28-29 0.4746927617299619 1104 30-31 0.45991429896328384 1104 32-33 0.5300344745635641 1104 34-35 0.5202603473593186 1104 36-37 0.34784156662547616 1104 38-39 0.3460085779335671 1104 40-41 0.2742490605531742 1104 42-43 0.4048912597712473 1104 44-45 0.44322562029965695 1104 46-47 0.4530341212843254 1104 48-49 0.32758892316801536 1104 50-51 0.35147076580660297 1104 52-53 0.26202342896083763 1104 54-55 0.2689067520461066 1104 56-57 -2.8911496153397778 1104 58-59 -10.286418696151916 1104 60-61 -10.307851632714705 1104 62-63 -3.9267380313669378 1104 64-65 -5.142845081812144 1104 66-67 -6.435170951269551 1104 68-69 -3.793096742466597 1104 70-71 -3.9570601514093475 1104 72-73 -4.109736259810553 1104 74-75 -5.033233642858011 1104 76-77 -6.369685382812079 1104 78-79 -9.162984113636117 1104 80-81 -4.692441612880327 1104 82-83 -2.2877492136948447 1104 84-85 -1.8692939786012772 1104 86-87 -1.8491858731238437 1104 88-89 -1.8323861027068808 1104 90-91 -4.355188461630249 1104 92-93 -6.7025752072980005 1104 94-95 -7.90207932558772 1104 96-97 -7.8684873856644355 1104 98-99 -8.363719902108386 1104 100-101 -8.267041474826103 1105 1 0.31449147925420107 1105 2 0.3385358685939117 1105 3 0.25457243383185 1105 4 0.19431051042577252 1105 5 0.1703027522719225 1105 6 0.16252377696500275 1105 7 0.21810153065422355 1105 8 0.231394222960148 1105 9 0.3292696045756358 1105 10-11 0.44071135098010217 1105 12-13 0.3303560204697291 1105 14-15 0.4697854564516888 1105 16-17 0.4709750749091839 1105 18-19 0.44146747423330623 1105 20-21 0.4757466306117806 1105 22-23 0.5635670741820817 1105 24-25 0.5351523512395531 1105 26-27 0.488555615924156 1105 28-29 0.587169960466376 1105 30-31 0.5724444993123967 1105 32-33 0.45318915773170687 1105 34-35 0.5690386677366988 1105 36-37 0.42033999620731777 1105 38-39 0.5274876098311765 1105 40-41 0.4691762324112787 1105 42-43 0.35543222352479376 1105 44-45 0.31294893980123106 1105 46-47 0.3586303969772686 1105 48-49 0.3423636889643049 1105 50-51 0.439547290471225 1105 52-53 0.4431473802537198 1105 54-55 0.5867537993579717 1105 56-57 1.7834493995248337 1105 58-59 -8.828122962253232 1105 60-61 -9.93837029351042 1105 62-63 -3.525975699054378 1105 64-65 -4.754054922897904 1105 66-67 -6.173375449913557 1105 68-69 -3.884894252145809 1105 70-71 -4.2231613165388175 1105 72-73 -2.4256117528312533 1105 74-75 -4.447470011930438 1105 76-77 -6.348099607780519 1105 78-79 -9.041849984825589 1105 80-81 -4.600544819553395 1105 82-83 -2.0482381724472134 1105 84-85 -1.6271364052597264 1105 86-87 -1.4839356783315942 1105 88-89 -1.5941431741186243 1105 90-91 -3.8732951564436426 1105 92-93 -6.2056815397072 1105 94-95 -7.821975919592347 1105 96-97 -8.324120203779813 1105 98-99 -8.786569108745216 1105 100-101 -8.744008348063158 1106 1 0.16200854016549826 1106 2 0.16742127808920415 1106 3 0.12282994831941707 1106 4 0.03516780033092459 1106 5 0.13579158664906998 1106 6 0.17392501083149625 1106 7 0.22007748830439766 1106 8 0.15901902995963013 1106 9 0.18370126401244136 1106 10-11 0.17613897662260314 1106 12-13 0.2038120163523871 1106 14-15 0.3945386497329295 1106 16-17 0.401593896386224 1106 18-19 0.26644232568138904 1106 20-21 0.3102653992851998 1106 22-23 0.47695663109968933 1106 24-25 0.5465598513565197 1106 26-27 0.3908798100007971 1106 28-29 0.3841371787858847 1106 30-31 0.34680642791390426 1106 32-33 0.3722513423304292 1106 34-35 0.33286577603756484 1106 36-37 0.35484556397974387 1106 38-39 0.4130884346368404 1106 40-41 0.16919536536273228 1106 42-43 0.24654108249906415 1106 44-45 0.20916604616373036 1106 46-47 0.2446706948163353 1106 48-49 0.2909887900598136 1106 50-51 0.08800270150383938 1106 52-53 0.056714815134839114 1106 54-55 0.03845270518709043 1106 56-57 1.273074577486348 1106 58-59 -8.98924580749857 1106 60-61 -9.989746863415043 1106 62-63 -4.0505356411709315 1106 64-65 -5.673595849033017 1106 66-67 -6.973210522201022 1106 68-69 -4.248731553547444 1106 70-71 -4.2139691448402115 1106 72-73 -4.625475482770897 1106 74-75 -5.177012375125658 1106 76-77 -6.823124422132405 1106 78-79 -9.351348033097441 1106 80-81 -5.0046803777676985 1106 82-83 -2.5590223895168123 1106 84-85 -2.1743588688486355 1106 86-87 -1.929834960469936 1106 88-89 -1.8934004981121824 1106 90-91 -4.368108789799479 1106 92-93 -6.742755601774832 1106 94-95 -8.082754705911535 1106 96-97 -7.820924416036933 1106 98-99 -7.290493034793434 1106 100-101 -7.715103805616413 1107 1 0.13368044788033018 1107 2 0.03498202004861639 1107 3 0.029058171804333455 1107 4 0.1432081502609961 1107 5 0.06320800888478573 1107 6 0.10378520860831486 1107 7 0.19814958889407563 1107 8 0.1261097596320795 1107 9 0.16060914705082752 1107 10-11 0.13939363549524586 1107 12-13 0.17091536138529762 1107 14-15 0.22386263461692835 1107 16-17 0.17566140097981275 1107 18-19 0.20371034321954085 1107 20-21 0.15899781587565087 1107 22-23 0.08792975197166442 1107 24-25 0.20821868715498937 1107 26-27 0.27966419386658714 1107 28-29 0.2619850463494302 1107 30-31 0.14527183939893007 1107 32-33 0.269448970162081 1107 34-35 0.270829841083156 1107 36-37 0.15652649067609303 1107 38-39 0.10031980755650238 1107 40-41 0.0625449077655631 1107 42-43 0.020800821156854 1107 44-45 0.03962741592755492 1107 46-47 0.17161454164423873 1107 48-49 -0.05070025051599458 1107 50-51 0.004916535788467513 1107 52-53 0.14564129201308162 1107 54-55 0.01129523825535017 1107 56-57 1.3537734364169793 1107 58-59 -8.566464629135794 1107 60-61 -10.005464000601279 1107 62-63 -3.694799697085415 1107 64-65 -5.163173358608677 1107 66-67 -7.085170444602539 1107 68-69 -3.798614491012099 1107 70-71 -3.8581745293163507 1107 72-73 -2.2476230095995646 1107 74-75 -4.580328052294178 1107 76-77 -6.656629297668857 1107 78-79 -7.313600872154275 1107 80-81 -2.2107516901977107 1107 82-83 -1.1168037440941418 1107 84-85 -0.9882338430171842 1107 86-87 -1.0190742181901378 1107 88-89 -0.8101413217390281 1107 90-91 -0.5956794798717091 1107 92-93 -3.064070034227786 1107 94-95 -5.701001282943729 1107 96-97 -6.456549152716313 1107 98-99 -6.158371163202584 1107 100-101 -7.128028983312776 1108 1 -0.10935334142716968 1108 2 -0.17660356309192693 1108 3 -0.07869702975434123 1108 4 -0.02461196666168064 1108 5 -0.09876955978990765 1108 6 -0.14624099363327758 1108 7 -0.1424420411721954 1108 8 0.023364702478097854 1108 9 -0.12254441132390781 1108 10-11 -0.12212583525256804 1108 12-13 -0.1457123880572908 1108 14-15 -0.17354055775386001 1108 16-17 -0.16851731595985342 1108 18-19 -0.21445440213328482 1108 20-21 -0.25499318820528316 1108 22-23 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84-85 1.414068419063824 2316 86-87 1.1010683889673238 2316 88-89 1.3462601498235465 2316 90-91 2.2522826976097683 2316 92-93 4.64294136546852 2316 94-95 11.593418751694946 2316 96-97 13.572914856557302 2316 98-99 13.282893798370464 2316 100-101 12.20652299108123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 19.0 4 33.0 5 112.0 6 269.0 7 593.0 8 1263.0 9 2272.0 10 3893.0 11 6148.0 12 9326.0 13 13062.0 14 15875.0 15 21166.0 16 29468.0 17 39511.0 18 43403.0 19 41425.0 20 43063.0 21 42970.0 22 43088.0 23 36523.0 24 37530.0 25 43982.0 26 61780.0 27 91856.0 28 129416.0 29 175697.0 30 221796.0 31 245714.0 32 249466.0 33 231047.0 34 208033.0 35 188179.0 36 155798.0 37 100849.0 38 39278.0 39 2978.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.47003186410651 19.377250603880906 14.24250646190417 22.91021107010841 2 15.19880156324587 21.75127780214151 39.334572649406795 23.715347985205824 3 19.211416623397188 26.207723000814166 30.789786384588457 23.79107399120019 4 12.744078795971722 17.238035254219923 37.787590666708574 32.23029528309978 5 13.11717767472807 38.65085632649381 34.26743938402322 13.964526614754902 6 30.478023255227342 37.022519066639816 17.85067463288011 14.648783045252733 7 27.02388893869066 33.93755168550532 21.47831314033272 17.560246235471304 8 25.483557005047263 34.6778343414148 20.490623794551627 19.347984858986308 9 26.46037868230727 16.728776587838873 20.589370957082647 36.22147377277121 10-11 24.47421943487539 26.500349453195977 27.994344329952114 21.03108678197652 12-13 26.476871476120568 24.09284783205136 28.059500567158075 21.37078012467 14-15 23.09593098274918 25.209392195083712 27.13539679835521 24.5592800238119 16-17 21.686005922659273 28.646197751314283 27.64931508337786 22.01848124264858 18-19 21.511521978423904 27.76383218540099 29.692715549951014 21.031930286224092 20-21 22.61759653038186 26.711515423866743 28.967360955076344 21.703527090675053 22-23 22.164891843887 26.63631209405804 28.787346078217936 22.411449983837027 24-25 22.39665999452051 26.65960257396342 29.10187583288641 21.84186159862966 26-27 22.56885419227503 26.370937838891383 29.357538089233227 21.70266987960036 28-29 21.83110384279322 26.890995347107992 29.600767933833815 21.677132876264974 30-31 21.905646434553923 27.044486377982462 29.230401418323005 21.81946576914061 32-33 21.788941693114054 27.044307427699476 28.944328150964722 22.22242272822175 34-35 22.400268087514974 26.60106905135712 28.788050779066275 22.210612082061633 36-37 22.546790288210694 26.440111344860206 29.190107945461996 21.822990421467104 38-39 22.671021490254095 26.34329628081386 29.413299280032202 21.572382948899847 40-41 22.17993535354477 26.630061650757607 29.460005105508003 21.729997890189615 42-43 21.822190308778115 27.133913681386744 28.87763888176535 22.16625712806979 44-45 22.10622632345799 27.142030111704713 28.37146187469645 22.380281690140848 46-47 22.188466970910717 27.125041352287298 27.868226699309478 22.818264977492504 48-49 22.50203045892354 27.007327912171508 27.994302865409278 22.49633876349568 50-51 22.45581134778886 27.326897345883626 28.1274330177112 22.089858288616313 52-53 22.396380254301196 27.39332049093714 28.015605084470245 22.194694170291417 54-55 21.70999933394605 26.97087402395425 28.457747226895062 22.861379415204635 56-57 21.330892733858324 27.732433253356426 28.553492222003513 22.383181790781737 58-59 20.764522908255042 26.856265499589306 29.82771314743548 22.551498444720167 60-61 22.065297457027008 26.795305622670806 28.708585978456508 22.430810941845678 62-63 22.894359532801516 26.77199517922386 28.02610716893498 22.30753811903964 64-65 22.019096786826626 27.023684203880627 28.575985323373565 22.381233685919184 66-67 21.18740493428156 27.043512513674038 29.070117863693852 22.698964688350546 68-69 21.26498176549613 27.053797776716383 29.185489552100524 22.495730905686962 70-71 21.144913672631297 27.047912213310244 28.88685623543048 22.92031787862798 72-73 20.477416288510252 26.831918081171846 29.389829745453806 23.300835884864096 74-75 21.143104298097498 27.158995757922472 28.757880901149495 22.940019042830535 76-77 19.74325260455042 27.16104446834669 29.669868942709826 23.42583398439306 78-79 20.423341285987917 27.088550977769188 29.23292818073382 23.255179555509077 80-81 21.441116626018303 27.599115449179774 27.982645999709966 22.977121925091957 82-83 21.83315731273889 27.42923457915038 27.480148932244138 23.257459175866597 84-85 21.770898738706297 27.00048275471326 27.402713671349595 23.825904835230848 86-87 22.000450158525652 26.955783566885405 27.68996883816413 23.353797436424813 88-89 22.142457295699934 26.581531330069048 27.69223604924953 23.58377532498149 90-91 21.102694396763415 26.860193825401534 28.447169692048103 23.589942085786948 92-93 20.190470254482985 27.359175401503855 28.851890337905022 23.598464006108134 94-95 19.090597988804966 27.483765697183866 28.969331324262694 24.456304989748475 96-97 19.009484416896107 27.381187367972437 28.43631458454962 25.17301363058183 98-99 19.47359329027607 27.093768580790606 28.306431398196253 25.12620673073707 100-101 20.161692424050738 26.978648468794443 28.19036245470736 24.669296652447457 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 995.0 1 725.0 2 476.5 3 553.5 4 612.5 5 733.0 6 979.5 7 1387.0 8 1920.5 9 2300.5 10 2461.0 11 2682.0 12 3023.0 13 3356.0 14 3652.5 15 3932.0 16 4181.0 17 4540.5 18 4913.5 19 5310.0 20 5882.5 21 6554.5 22 7576.0 23 8813.5 24 10438.0 25 12750.5 26 15664.0 27 19099.0 28 22911.0 29 26961.0 30 31113.0 31 35354.5 32 39773.5 33 44607.0 34 49535.5 35 54111.5 36 59133.0 37 64026.0 38 67776.0 39 71118.0 40 74648.0 41 78571.5 42 82988.0 43 87668.5 44 92939.0 45 99181.5 46 106360.5 47 114809.5 48 123371.0 49 121800.0 50 111383.5 51 102733.5 52 94754.5 53 87526.5 54 80701.0 55 72934.0 56 64733.0 57 56523.5 58 48387.0 59 40556.0 60 33077.5 61 26244.0 62 20524.5 63 15600.0 64 11495.5 65 8264.0 66 5891.0 67 4155.5 68 2824.5 69 1799.0 70 1123.5 71 715.5 72 453.0 73 312.0 74 197.0 75 95.5 76 49.5 77 25.5 78 14.0 79 5.5 80 0.5 81 1.0 82 1.0 83 1.0 84 2.0 85 2.0 86 1.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024448141417363532 2 0.007489668719922479 3 3.492591631051934E-4 4 0.0 5 0.14742617340406994 6 0.0 7 0.0 8 0.0015910695208125475 9 0.0 10-11 0.0 12-13 0.0 14-15 7.761314735670963E-4 16-17 0.0 18-19 1.3582300787424187E-4 20-21 0.0 22-23 2.3283944207012891E-4 24-25 3.880657367835482E-5 26-27 2.522427289093063E-4 28-29 0.00382244750731795 30-31 0.012379297003395188 32-33 0.0026776535838064826 34-35 0.004365739538814917 36-37 0.11246145051987226 38-39 0.11172412561998352 40-41 0.12387058318130857 42-43 0.12631539732304492 44-45 0.12158099533428565 46-47 0.11496447452212614 48-49 0.11477044165373437 50-51 0.11327638856711772 52-53 0.09071036597315438 54-55 0.07825345582240249 56-57 0.5375292553057318 58-59 1.541358299930575 60-61 0.5080750658838604 62-63 0.004637385554563401 64-65 0.11620628487983349 66-67 1.877035162248344 68-69 2.8266320201576867 70-71 2.7389873735051222 72-73 2.5918328461168008 74-75 1.979057644448739 76-77 1.3781572543262537 78-79 0.9176784510588956 80-81 0.05194259886847792 82-83 2.522427289093063E-4 84-85 1.9403286839177409E-4 86-87 5.044854578186126E-4 88-89 2.1343615523095147E-4 90-91 0.2433366202501239 92-93 0.38090592393989176 94-95 1.966464911290113 96-97 2.388408786894865 98-99 1.3821349281282853 100-101 0.922199416892424 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2576883.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.73636395245191 #Duplication Level Percentage of deduplicated Percentage of total 1 67.1400111828858 28.693199536834985 2 15.567454762031563 13.305928250470231 3 6.554007873224597 8.402833974520851 4 3.3428868826894815 5.714513218819804 5 1.9735522027801222 4.217122260858723 6 1.2422115496851596 3.1852562935970568 7 0.8208428249169342 2.4555886399386204 8 0.6123422110892134 2.0935423677246208 9 0.45782947166097254 1.7609370236155881 >10 2.1008951555672657 15.505093237114304 >50 0.11665758290137689 3.410686671992104 >100 0.06248516311532143 4.783126145021685 >500 0.005275690435400728 1.52965544390569 >1k 0.002910725757460772 2.5641845500967038 >5k 4.548008996032456E-4 1.468585845369425 >10k+ 1.8192035984129824E-4 0.9097465401196605 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12016 0.46629978931911154 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11387 0.4418904544754263 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9378 0.36392804795561146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9128 0.3542264045360228 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6654 0.25821894125577294 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6459 0.25065165938849376 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6160 0.23904849385866567 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4550 0.17656991023651442 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4477 0.17373703035799454 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4169 0.16178460566506125 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3630 0.140867862452428 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3436 0.13333938715882715 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2954 0.11463461864586012 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.940328683917741E-5 0.0 18-19 0.0 0.0 0.0 1.3582300787424187E-4 0.0 20-21 0.0 0.0 0.0 2.5224272890930636E-4 0.0 22-23 0.0 0.0 0.0 7.373248998887416E-4 0.0 24-25 0.0 0.0 0.0 0.0014746497997774831 0.0 26-27 0.0 0.0 0.0 0.002464217428575531 0.0 28-29 0.0 0.0 0.0 0.0061508419280192385 0.0 30-31 0.0 0.0 0.0 0.01684205297640599 0.0 32-33 0.0 0.0 0.0 0.037622973181165 0.0 34-35 0.0 0.0 0.0 0.06899808800011487 0.0 36-37 0.0 0.0 0.0 0.11331519514079608 0.0 38-39 0.0 0.0 0.0 0.17261163972132224 0.0 40-41 0.0 0.0 0.0 0.24038732065056895 0.0 42-43 0.0 0.0 0.0 0.31336308245271516 0.0 44-45 0.0 0.0 0.0 0.39714647502428324 0.0 46-47 0.0 0.0 0.0 0.4965495135013891 0.0 48-49 0.0 0.0 0.0 0.5876673484981663 0.0 50-51 0.0 5.820986051753223E-5 0.0 0.6734686829010087 0.0 52-53 0.0 1.1641972103506446E-4 0.0 0.7544968087414136 0.0 54-55 0.0 1.1641972103506446E-4 0.0 0.8430728131622585 0.0 56-57 3.880657367835482E-5 1.1641972103506446E-4 0.0 0.9198322158980443 0.0 58-59 3.880657367835482E-5 1.1641972103506446E-4 0.0 0.9749569538081473 0.0 60-61 3.880657367835482E-5 1.1641972103506446E-4 0.0 1.022495006564132 0.0 62-63 3.880657367835482E-5 1.1641972103506446E-4 0.0 1.0642120732683633 0.0 64-65 3.880657367835482E-5 1.1641972103506446E-4 0.0 1.104648523041209 0.0 66-67 3.880657367835482E-5 1.1641972103506446E-4 0.0 1.1375176909467757 0.0 68-69 3.880657367835482E-5 1.1641972103506446E-4 0.0 1.1849393239817252 0.0 70-71 5.820986051753223E-5 1.1641972103506446E-4 0.0 1.2603210933519295 0.0 72-73 7.761314735670964E-5 1.1641972103506446E-4 0.0 1.3653122784387184 0.0 74-75 7.761314735670964E-5 1.1641972103506446E-4 0.0 1.500009895676288 0.0 76-77 7.761314735670964E-5 1.1641972103506446E-4 0.0 1.7113698992154476 0.0 78-79 7.761314735670964E-5 1.1641972103506446E-4 0.0 2.0278763141361096 0.0 80-81 7.761314735670964E-5 1.1641972103506446E-4 0.0 2.4058911483369636 0.0 82-83 7.761314735670964E-5 1.1641972103506446E-4 0.0 2.784623904150868 0.0 84-85 7.761314735670964E-5 1.1641972103506446E-4 0.0 3.0980451964641 0.0 86-87 7.761314735670964E-5 1.1641972103506446E-4 0.0 3.3438460341428 0.0 88-89 7.761314735670964E-5 1.1641972103506446E-4 0.0 3.5701465685481257 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11780 0.0 25.553988 1 TACATGG 11800 0.0 24.78773 2 ACATGGG 11675 0.0 23.680994 3 GAGTACT 9040 0.0 21.719456 12-13 CATGGGA 8315 0.0 21.581491 4 AGTACTT 9445 0.0 20.115938 12-13 GTACTTT 10150 0.0 19.436892 14-15 CATGGGG 3910 0.0 19.27356 4 ATGGGAG 3330 0.0 18.811617 5 ATGGGAT 3575 0.0 18.708157 5 ACTTTTT 11820 0.0 16.969242 16-17 TACTTTT 11595 0.0 16.487343 14-15 AGTACAT 9015 0.0 16.196571 2 GTATCAA 31520 0.0 14.99056 1 GCTTATA 2435 0.0 14.896865 1 CGCGTAG 150 2.0815312E-5 14.572267 66-67 GTGTAGC 1235 0.0 14.113603 1 GTGTAGG 1135 0.0 14.111922 1 AACGCAG 33600 0.0 13.99657 6 ATCAACG 33605 0.0 13.973654 3 >>END_MODULE