FastQCFastQC Report
Fri 27 May 2016
SRR939604_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939604_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2593221
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51130.19716792359771884No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA49970.19269472212356756No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA38070.14680584493184345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.1393248010871422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26700.10296075806882639No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG103600.033.1887361
TACATGG120150.026.656642
ACATGGG118800.025.8471813
CATGGGA84300.022.6366064
ATAACGC2103.6565325E-822.495573
GAGTACT92550.021.95297412-13
CATGGGG43150.020.6889534
AGTACTT98700.020.44163312-13
ATGGGAT37550.020.2354355
GTACTTT102350.019.80888614-15
GCTTATA18500.017.9335631
GTATACA11000.017.365421
GTGTAGC10750.016.8340441
ACTTTTT127550.016.73263716-17
GTATATG10401.8189894E-1216.4338741
GTATCAA314600.016.361971
GTCCTAT6151.05053E-616.3474241
TACTTTT123050.016.34228114-15
ATGGGAG35850.015.7975125
TATAACG2406.4488406E-515.7462882
Produced by FastQC (version 0.11.5)