##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939604_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2593221 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.332468385841391 2.0 2.0 31.0 2.0 33.0 2 22.63999250353132 16.0 16.0 31.0 16.0 34.0 3 27.14660416524469 28.0 25.0 31.0 16.0 34.0 4 32.38378950347849 35.0 32.0 35.0 25.0 37.0 5 32.996468484560324 35.0 33.0 37.0 28.0 37.0 6 33.40020383916373 35.0 33.0 37.0 28.0 37.0 7 33.56799594018404 35.0 33.0 37.0 28.0 37.0 8 33.50344571480795 35.0 33.0 37.0 28.0 37.0 9 34.87337060744148 37.0 34.0 39.0 28.0 39.0 10-11 34.71816247053375 37.0 34.0 39.0 27.0 39.0 12-13 34.72757817401602 37.0 34.0 39.0 27.0 39.0 14-15 35.40159708717459 38.0 34.0 40.0 27.0 41.0 16-17 35.03347400780728 38.0 33.0 40.0 26.0 41.0 18-19 35.32803162553442 38.0 34.0 40.0 27.0 41.0 20-21 35.33102057248495 38.0 34.0 40.0 26.5 41.0 22-23 35.16081371391023 38.0 34.0 40.0 25.5 41.0 24-25 34.95617072359047 38.0 34.0 40.0 25.0 41.0 26-27 35.03883105219339 38.0 34.0 40.0 25.0 41.0 28-29 34.78038663114327 38.0 33.5 40.0 24.0 41.0 30-31 34.661851997959296 38.0 33.0 40.0 24.0 41.0 32-33 34.354457834484606 38.0 33.0 40.0 24.0 41.0 34-35 34.081427306041405 38.0 33.0 40.0 23.0 41.0 36-37 33.785226558014145 38.0 32.5 40.0 21.0 41.0 38-39 33.572694344215165 38.0 32.0 40.0 19.0 41.0 40-41 33.18304995987616 37.0 31.0 40.0 17.0 41.0 42-43 32.9356406569282 37.0 31.0 40.0 16.0 41.0 44-45 32.591908865461136 37.0 30.5 40.0 15.0 41.0 46-47 32.22515917463262 37.0 30.0 40.0 11.5 41.0 48-49 31.84690892137616 36.5 30.0 39.0 9.0 40.5 50-51 31.16037507023119 35.5 29.5 38.5 8.0 40.0 52-53 31.16354043870538 36.0 29.5 39.0 8.0 40.0 54-55 31.29185885044121 36.0 29.5 39.0 7.0 40.0 56-57 31.0313064331964 36.0 29.0 39.0 4.0 40.0 58-59 30.64056245109846 35.0 28.0 39.0 2.0 40.0 60-61 30.034096785426307 35.0 28.0 38.0 2.0 40.0 62-63 29.62983447997683 34.0 27.0 38.0 2.0 40.0 64-65 29.026935421238683 34.0 26.0 38.0 2.0 40.0 66-67 28.4383685385858 34.0 26.0 37.0 2.0 39.0 68-69 28.117162401507624 33.5 26.0 37.0 2.0 39.0 70-71 27.588626268258665 33.0 25.0 36.0 2.0 39.0 72-73 26.996127402947916 32.0 23.5 36.0 2.0 38.5 74-75 26.437062055258693 32.0 22.0 35.0 2.0 38.0 76-77 25.783962107356064 31.5 20.5 35.0 2.0 37.0 78-79 25.132616541359184 31.0 19.0 34.5 2.0 36.0 80-81 24.416030874345072 30.5 15.5 34.0 2.0 36.0 82-83 23.738711046995224 30.0 9.0 34.0 2.0 35.0 84-85 23.062627327173427 29.5 6.0 34.0 2.0 35.0 86-87 22.362882299657453 29.0 2.0 34.0 2.0 35.0 88-89 21.53078642352503 29.0 2.0 33.5 2.0 35.0 90-91 20.55001077810183 27.0 2.0 33.0 2.0 34.0 92-93 19.503990982642822 25.5 2.0 32.0 2.0 34.0 94-95 18.528077244477043 25.0 2.0 32.0 2.0 34.0 96-97 17.610605497950232 23.5 2.0 32.0 2.0 34.0 98-99 16.550774114508556 19.0 2.0 31.0 2.0 34.0 100-101 14.983629817898283 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 15.084994358095667 1101 2 8.213660427019057 1101 3 3.9365900368876936 1101 4 2.5595005063462466 1101 5 1.9319886715313714 1101 6 1.6219873626226544 1101 7 1.212298278241228 1101 8 1.3510195716273685 1101 9 1.3172409021026041 1101 10-11 1.3831223789990048 1101 12-13 1.1830505922951886 1101 14-15 1.355713743799221 1101 16-17 1.3888987931368817 1101 18-19 1.4158473752284095 1101 20-21 1.3725808691594281 1101 22-23 1.3574871349908264 1101 24-25 1.5224095676480758 1101 26-27 1.5646783192182596 1101 28-29 1.635902608634943 1101 30-31 1.5684030165571272 1101 32-33 1.5342481127321363 1101 34-35 1.6672755690240493 1101 36-37 1.6284820924011711 1101 38-39 1.504565264407482 1101 40-41 1.5094335047970517 1101 42-43 1.4665954281901747 1101 44-45 1.3568354340402138 1101 46-47 1.1956836263905402 1101 48-49 1.203826808812675 1101 50-51 1.113588911809547 1101 52-53 1.2201184361934843 1101 54-55 1.4073910948834154 1101 56-57 1.2891487322355601 1101 58-59 1.278403134262252 1101 60-61 1.1832096450548946 1101 62-63 0.9064822548163995 1101 64-65 0.8369594173043744 1101 66-67 0.7755573492631633 1101 68-69 0.8940754395289758 1101 70-71 0.672868003010624 1101 72-73 0.9302421015687301 1101 74-75 0.5018978371209819 1101 76-77 0.5762960725131698 1101 78-79 0.584729287187475 1101 80-81 0.3334302003621623 1101 82-83 0.5946849116417106 1101 84-85 0.3606249921879616 1101 86-87 0.3317966392538345 1101 88-89 0.3277090788521946 1101 90-91 0.3299458040839589 1101 92-93 0.3161899297949482 1101 94-95 0.32584147180964607 1101 96-97 -0.7017512829307115 1101 98-99 -0.8833538682729163 1101 100-101 -0.920667033313924 1102 1 14.894445713544211 1102 2 8.104480186050427 1102 3 3.8543410376209053 1102 4 2.254195753358907 1102 5 1.6455467487364501 1102 6 1.3553043473441306 1102 7 0.9649375882307254 1102 8 0.9482157846943693 1102 9 1.092850011747295 1102 10-11 1.132139604490952 1102 12-13 0.7974397858627498 1102 14-15 0.9262775108166394 1102 16-17 1.1856960753040156 1102 18-19 1.1501378454739353 1102 20-21 1.0477800869031242 1102 22-23 1.001366994987258 1102 24-25 0.9122886622322213 1102 26-27 1.0649207617008614 1102 28-29 1.1890907117996292 1102 30-31 1.222293614619069 1102 32-33 1.0562893236193247 1102 34-35 1.0640715507334235 1102 36-37 1.250104927448625 1102 38-39 1.176707787146917 1102 40-41 1.214576381756416 1102 42-43 1.16791495933181 1102 44-45 1.135635619324482 1102 46-47 1.0354068518230406 1102 48-49 0.8839278234174337 1102 50-51 0.6873425159353381 1102 52-53 0.992599635289853 1102 54-55 0.8070022580128366 1102 56-57 0.8084134658027224 1102 58-59 0.8236507042640362 1102 60-61 0.7864678490855717 1102 62-63 0.5334598795117174 1102 64-65 0.6238958236813232 1102 66-67 0.5953222636558912 1102 68-69 0.3570997097863895 1102 70-71 0.5019729908234787 1102 72-73 0.6706927163756156 1102 74-75 0.5204507248803303 1102 76-77 0.25770621459746934 1102 78-79 0.3108640294739011 1102 80-81 0.42179940155908113 1102 82-83 0.38366210780523247 1102 84-85 0.1177731750149853 1102 86-87 0.2722093550059732 1102 88-89 0.1732348229617422 1102 90-91 0.516915093464295 1102 92-93 0.5965166047845436 1102 94-95 0.35227265653904993 1102 96-97 -0.334284339328466 1102 98-99 -0.5307161683739814 1102 100-101 -0.5057993332168227 1103 1 14.811410681089075 1103 2 7.904109497492453 1103 3 3.7180254909746004 1103 4 2.2575247911595966 1103 5 1.7174293056920362 1103 6 1.2131222167599418 1103 7 0.9417019384916401 1103 8 1.0390663001582823 1103 9 1.0773940489116072 1103 10-11 0.9964365689812169 1103 12-13 0.773247584640913 1103 14-15 0.9205799640430641 1103 16-17 1.0211970616855837 1103 18-19 0.9056265816327738 1103 20-21 0.9880343983451425 1103 22-23 1.042777122898677 1103 24-25 1.2149447332516914 1103 26-27 1.0301567773556997 1103 28-29 1.0585741342254948 1103 30-31 1.0337077994224373 1103 32-33 0.8633264402032594 1103 34-35 0.8194190911432528 1103 36-37 0.8683056560982436 1103 38-39 0.8654188887123979 1103 40-41 0.7576112551177445 1103 42-43 0.7745953080654147 1103 44-45 0.7702090888421083 1103 46-47 0.7222565559085652 1103 48-49 0.6180741260774845 1103 50-51 0.6550723269319043 1103 52-53 0.823415309211196 1103 54-55 0.6325499384252069 1103 56-57 0.7088247232801344 1103 58-59 0.5776539020494482 1103 60-61 0.493238085817147 1103 62-63 0.42789119871752135 1103 64-65 0.3221326666239186 1103 66-67 0.3840357516283994 1103 68-69 0.27081916377646564 1103 70-71 0.10582942457682165 1103 72-73 0.18004544324337957 1103 74-75 0.2570212193438657 1103 76-77 0.19332095304725883 1103 78-79 -0.08659206063682845 1103 80-81 -0.19997099426630172 1103 82-83 -0.11494979877488376 1103 84-85 -0.2330326350906553 1103 86-87 -0.28308568444928994 1103 88-89 -0.37317085032322694 1103 90-91 -0.2578995622567142 1103 92-93 0.05337036576201726 1103 94-95 -0.1810992570411969 1103 96-97 -0.7601963445467455 1103 98-99 -0.9557949198058253 1103 100-101 -1.0039896412224234 1104 1 14.916253865153767 1104 2 8.169970263355147 1104 3 4.089649246920548 1104 4 2.4959078509705392 1104 5 1.909189733621652 1104 6 1.5344234196874993 1104 7 1.192671809991424 1104 8 1.1196912087165174 1104 9 1.3539262203546514 1104 10-11 1.1025554058062852 1104 12-13 0.9954483961085145 1104 14-15 1.1406144353580103 1104 16-17 1.3525385525164282 1104 18-19 1.236179728478433 1104 20-21 1.1833240323282936 1104 22-23 1.2259652556310812 1104 24-25 1.2208794375870227 1104 26-27 1.2400278489282783 1104 28-29 1.4305195510750437 1104 30-31 1.4593968510330555 1104 32-33 1.4149020933204852 1104 34-35 1.4437694710057158 1104 36-37 1.560899247859389 1104 38-39 1.497060201477133 1104 40-41 1.5334658475461893 1104 42-43 1.3877883295867974 1104 44-45 1.218289507636463 1104 46-47 1.1598143024980132 1104 48-49 1.0854095933493646 1104 50-51 1.3985533587875416 1104 52-53 1.3823638270517087 1104 54-55 1.3738638450671772 1104 56-57 1.1632102524647792 1104 58-59 1.226266877680441 1104 60-61 1.1166249103524457 1104 62-63 0.9128824738350154 1104 64-65 1.30668751919303 1104 66-67 1.1071349583599392 1104 68-69 1.1581878890871877 1104 70-71 1.082755268428187 1104 72-73 1.1568341868959813 1104 74-75 1.0649849495255346 1104 76-77 0.8062506936559686 1104 78-79 0.7748333960367262 1104 80-81 0.73466082996568 1104 82-83 0.7133850511535691 1104 84-85 0.7216068256438071 1104 86-87 0.45752787640899584 1104 88-89 0.5037363661716192 1104 90-91 0.5178720278129738 1104 92-93 0.7808087473944418 1104 94-95 0.9516387145752816 1104 96-97 0.5255152597254131 1104 98-99 0.2956134439486213 1104 100-101 0.2859747812403146 1105 1 14.868556793456023 1105 2 8.108669269992724 1105 3 4.0121426250875665 1105 4 2.4716171831188802 1105 5 1.8231272487110317 1105 6 1.3499155347202034 1105 7 1.1078376094924707 1105 8 1.1316408093071502 1105 9 1.1081050255905396 1105 10-11 1.103199219422244 1105 12-13 0.989566598888814 1105 14-15 1.1423454542280425 1105 16-17 1.445807971019768 1105 18-19 1.2044575620456186 1105 20-21 1.203368329504265 1105 22-23 1.14229488820245 1105 24-25 1.2421116408641666 1105 26-27 1.2305169023473184 1105 28-29 1.386110687441068 1105 30-31 1.038209504769803 1105 32-33 0.8541148912154952 1105 34-35 1.1072536223439116 1105 36-37 1.1836621879882188 1105 38-39 1.2530700266327486 1105 40-41 1.5152954678711055 1105 42-43 1.4128470950741843 1105 44-45 1.3166870415504022 1105 46-47 1.1678914341683964 1105 48-49 1.1631907457057586 1105 50-51 1.243844666953823 1105 52-53 1.446668630811022 1105 54-55 1.3286426566012395 1105 56-57 1.3537077734324612 1105 58-59 1.3346608076377215 1105 60-61 1.305193929621364 1105 62-63 1.4430960869940037 1105 64-65 1.3105772031295366 1105 66-67 1.1364097932952255 1105 68-69 0.9508011775401783 1105 70-71 1.0661464866705082 1105 72-73 0.9548710452254952 1105 74-75 0.9451379952188077 1105 76-77 0.9843604708754619 1105 78-79 0.8777891303516405 1105 80-81 0.8443361589579332 1105 82-83 0.9133527205132879 1105 84-85 0.7397059659426866 1105 86-87 0.6806207010362648 1105 88-89 0.8326094474145975 1105 90-91 1.006433139371179 1105 92-93 1.291955960276315 1105 94-95 1.346016402234703 1105 96-97 1.0394538944906486 1105 98-99 0.7245742797866477 1105 100-101 0.5866535847267045 1106 1 14.817678661839043 1106 2 8.056495526473359 1106 3 3.6243989461895403 1106 4 2.287416783795287 1106 5 1.6878284074578787 1106 6 1.074822132455978 1106 7 0.9322016757505907 1106 8 0.8307561220614375 1106 9 0.9595915470119323 1106 10-11 0.9588608182789926 1106 12-13 0.7639609171425548 1106 14-15 0.8173265115500499 1106 16-17 1.0820603645107099 1106 18-19 0.8445364520012504 1106 20-21 0.8186382491082256 1106 22-23 0.8115120719949616 1106 24-25 0.9013906974357369 1106 26-27 0.8642480902670542 1106 28-29 1.0689533508413191 1106 30-31 0.8242256828258903 1106 32-33 0.8000190785392576 1106 34-35 0.7953792301124736 1106 36-37 0.7535294018405168 1106 38-39 0.7763307702785625 1106 40-41 1.023355870816431 1106 42-43 0.7372142503720198 1106 44-45 0.7712203583539363 1106 46-47 0.8517973450390244 1106 48-49 0.8141243152888471 1106 50-51 0.7716556347472228 1106 52-53 0.7436195289458816 1106 54-55 0.655204265713607 1106 56-57 0.7520824854824681 1106 58-59 0.6583305264129145 1106 60-61 0.6398438116818319 1106 62-63 0.6843869513855729 1106 64-65 0.6660342389117311 1106 66-67 0.5931965302492159 1106 68-69 0.596064231238536 1106 70-71 0.735012961487211 1106 72-73 0.40298973079928047 1106 74-75 0.32807471644615305 1106 76-77 0.24775938658133612 1106 78-79 0.3414150012377455 1106 80-81 0.35594559395609693 1106 82-83 0.29333467228390475 1106 84-85 0.12756339014760698 1106 86-87 0.35835217067642944 1106 88-89 0.43591091696003303 1106 90-91 0.7465821573929112 1106 92-93 1.1161352333140648 1106 94-95 0.79653183940178 1106 96-97 0.13827975735897624 1106 98-99 -0.009596348058618531 1106 100-101 -0.033596210126736636 1107 1 14.692006848827429 1107 2 7.877638965448632 1107 3 3.571490754717747 1107 4 2.0374298803430335 1107 5 1.3893792248553112 1107 6 1.0497521490254371 1107 7 0.8936461039739285 1107 8 0.7409618030114515 1107 9 0.7410580306194419 1107 10-11 0.7087257310453481 1107 12-13 0.5044180544685588 1107 14-15 0.6672864108515526 1107 16-17 0.8543353983955413 1107 18-19 0.6290443262839744 1107 20-21 0.45193531098748707 1107 22-23 0.44012721777350094 1107 24-25 0.8147399487253821 1107 26-27 0.7291060106735543 1107 28-29 0.7495128619531712 1107 30-31 0.6062683480544635 1107 32-33 0.8054112662956214 1107 34-35 0.760367371421161 1107 36-37 0.7213701972809474 1107 38-39 0.8334827750608582 1107 40-41 0.9609718284479669 1107 42-43 0.8317374868387688 1107 44-45 0.7917988907056 1107 46-47 0.6460231901519435 1107 48-49 0.5907543169402096 1107 50-51 0.5116637665732178 1107 52-53 0.4098361929134633 1107 54-55 0.5012406520450092 1107 56-57 0.44897285979909185 1107 58-59 0.45653559468382454 1107 60-61 0.5690422790267498 1107 62-63 0.503877167267202 1107 64-65 0.1777158276652422 1107 66-67 -0.0014152574947026153 1107 68-69 0.32675952097538996 1107 70-71 0.08126421097764691 1107 72-73 0.19928476397675965 1107 74-75 0.1653956494096427 1107 76-77 0.21572396766598345 1107 78-79 0.30261026265414515 1107 80-81 0.2592934704406282 1107 82-83 0.38638790436563397 1107 84-85 0.3699523015067143 1107 86-87 0.058450866792110645 1107 88-89 0.23820337124776003 1107 90-91 0.6349787623422607 1107 92-93 0.8704335601735806 1107 94-95 0.890926902548415 1107 96-97 0.4836735486114385 1107 98-99 0.18732713326703632 1107 100-101 0.20449490621235888 1108 1 14.30048103984823 1108 2 7.435304555231493 1108 3 3.261482460436003 1108 4 -0.24032507498201028 1108 5 0.17360382420396547 1108 6 0.47554491501460205 1108 7 0.3967839595241358 1108 8 0.37488890821328624 1108 9 0.5344582117277668 1108 10-11 0.48573297589974374 1108 12-13 0.13797176059449612 1108 14-15 0.20543111400646552 1108 16-17 0.5143717660318785 1108 18-19 0.3071358751355149 1108 20-21 0.35805444210543413 1108 22-23 -0.30151137988293897 1108 24-25 0.16214471436631328 1108 26-27 0.20185387337112815 1108 28-29 0.015702872426594183 1108 30-31 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2312 5 -1.8048602148045667 2312 6 -0.6903695037645008 2312 7 -0.21748478676411054 2312 8 -0.0022967261442303766 2312 9 -0.05549271503745956 2312 10-11 0.021645519268602698 2312 12-13 -0.5858443866877252 2312 14-15 0.1832215681683067 2312 16-17 -0.10671097081679903 2312 18-19 0.18379014325667242 2312 20-21 0.14506566331007065 2312 22-23 0.341251099409412 2312 24-25 0.21105228789802055 2312 26-27 0.18243400027388645 2312 28-29 -0.41930791118232236 2312 30-31 0.2023978199950207 2312 32-33 0.3435161651486993 2312 34-35 -0.6047123844001518 2312 36-37 0.07524363569422832 2312 38-39 0.2832887514724405 2312 40-41 0.31489741070063104 2312 42-43 0.272188979729016 2312 44-45 0.3885870727288321 2312 46-47 0.38646013127306134 2312 48-49 0.451142097334607 2312 50-51 0.4427405247300875 2312 52-53 0.4214264344970857 2312 54-55 0.21027899838196618 2312 56-57 0.32897557612932715 2312 58-59 0.32188904502954685 2312 60-61 0.4969030962055996 2312 62-63 0.22202322337397362 2312 64-65 0.20625742510994982 2312 66-67 0.14824520122556706 2312 68-69 0.38422639751090415 2312 70-71 0.3079682837752955 2312 72-73 0.07566518117597809 2312 74-75 0.3187920323248967 2312 76-77 0.30822956403386215 2312 78-79 0.41410167347241256 2312 80-81 0.5660167515735317 2312 82-83 0.4346393880397592 2312 84-85 0.4797186000944649 2312 86-87 0.5245015894087146 2312 88-89 0.8630340250344943 2312 90-91 1.031775069103258 2312 92-93 1.5131770779667555 2312 94-95 1.6582789171195884 2312 96-97 1.3906507581156866 2312 98-99 1.3854434067529837 2312 100-101 1.198590040828348 2313 1 -14.23402650867801 2313 2 -7.470876136495818 2313 3 -3.6916827616490195 2313 4 -1.3881515150058021 2313 5 -0.6744132098378444 2313 6 -1.1835260943932866 2313 7 -0.47058648789412416 2313 8 -0.2957010973039118 2313 9 -2.743157022418657 2313 10-11 -0.812899309007264 2313 12-13 -0.9957522745531406 2313 14-15 -1.054309959959788 2313 16-17 -0.9595110019282558 2313 18-19 -0.27959944996104014 2313 20-21 -1.1776673670569338 2313 22-23 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0.14086566012061041 2313 88-89 0.5091184259722823 2313 90-91 0.9556914459518886 2313 92-93 1.4033703023359152 2313 94-95 1.4909876138828864 2313 96-97 1.3218955497244806 2313 98-99 1.0453874061307538 2313 100-101 0.9224047498806716 2314 1 -14.23402650867801 2314 2 -7.302372499968927 2314 3 -3.492804024107489 2314 4 -1.3941338262206706 2314 5 -1.2550183997453601 2314 6 -6.0938823393425565 2314 7 -1.9548989149391112 2314 8 -4.1542491802706 2314 9 -1.4189034083394603 2314 10-11 -0.44306059570924816 2314 12-13 -3.1594139428659744 2314 14-15 -2.978449090784963 2314 16-17 -2.617980517955907 2314 18-19 -1.33759363600754 2314 20-21 -2.292978379897015 2314 22-23 -1.417167164171893 2314 24-25 -3.082431057487497 2314 26-27 -5.073902436497331 2314 28-29 -2.290968514092924 2314 30-31 -2.172313492488037 2314 32-33 -4.251707214585551 2314 34-35 -2.8744617742582363 2314 36-37 -1.1767229420875722 2314 38-39 -1.6371154110512833 2314 40-41 -2.702519881173181 2314 42-43 -1.6676685408350238 2314 44-45 -2.764704303932106 2314 46-47 -2.475244424453237 2314 48-49 -1.4646102980373534 2314 50-51 -2.4065419935741694 2314 52-53 -1.3134188832286675 2314 54-55 -0.7276605297749512 2314 56-57 -1.5538404547845275 2314 58-59 -1.842462283723954 2314 60-61 -0.8386823010645799 2314 62-63 -2.9406360914592504 2314 64-65 -1.3137908212969904 2314 66-67 -2.2848860696774445 2314 68-69 -1.8650787186420388 2314 70-71 -2.7192982804477914 2314 72-73 -2.718257692366457 2314 74-75 -2.0305221229675183 2314 76-77 -0.8157422338509797 2314 78-79 -0.495141976532107 2314 80-81 -0.12536442893808797 2314 82-83 -0.21130994758349075 2314 84-85 -0.03368870353022757 2314 86-87 0.10418612344107103 2314 88-89 0.37458096076621317 2314 90-91 0.682541136173672 2314 92-93 1.340297886065823 2314 94-95 1.0990121043201668 2314 96-97 1.0066743276718633 2314 98-99 1.1335355833777676 2314 100-101 0.8916564559056344 2315 1 -14.23402650867801 2315 2 -7.393184264033966 2315 3 -4.5551192743594235 2315 4 -2.7805556275988046 2315 5 -3.456143239734832 2315 6 -2.8120823456975828 2315 7 -2.9807553684702377 2315 8 -3.1235947939393576 2315 9 -2.0738786010329946 2315 10-11 -4.4907706861322225 2315 12-13 -4.339746038481913 2315 14-15 -3.1556832236413612 2315 16-17 -4.232868760245903 2315 18-19 -1.339447431775099 2315 20-21 -3.1759782823019336 2315 22-23 -4.085503292961086 2315 24-25 -5.031202097170109 2315 26-27 -4.645769180634819 2315 28-29 -2.5412595403391904 2315 30-31 -3.1661151270920698 2315 32-33 -4.218326804867804 2315 34-35 -3.6613013052571546 2315 36-37 -5.0354849939446 2315 38-39 -3.475082675847151 2315 40-41 -4.628358858376789 2315 42-43 -3.215625569496204 2315 44-45 -1.9343459938248913 2315 46-47 -1.4717236253538317 2315 48-49 -1.0175921113084634 2315 50-51 -1.0266625802340563 2315 52-53 -0.8002827562552035 2315 54-55 -2.343694946791903 2315 56-57 -4.6710441402469485 2315 58-59 -3.832693186864244 2315 60-61 -4.530729040027257 2315 62-63 -2.5586970815726424 2315 64-65 -3.2909428494588404 2315 66-67 -2.091161262824908 2315 68-69 -1.5374930165476002 2315 70-71 -2.1693155298100173 2315 72-73 -2.3587055743263505 2315 74-75 -2.7705384871651084 2315 76-77 -2.631675651312783 2315 78-79 -1.906592489019733 2315 80-81 -1.7260988886245094 2315 82-83 -1.2140856196277205 2315 84-85 -0.9807383816934454 2315 86-87 -1.4941888050648586 2315 88-89 -2.0318565125653656 2315 90-91 -1.6718249951575253 2315 92-93 -0.5984161512835833 2315 94-95 -0.5325405538973413 2315 96-97 -1.4369039730843838 2315 98-99 -0.8229477369428864 2315 100-101 -0.3948407546909234 2316 1 -14.23402650867801 2316 2 -7.3464255120826145 2316 3 -5.200811902082684 2316 4 -2.987233689409372 2316 5 -2.26438952078259 2316 6 -1.2821427293000625 2316 7 -4.528549941929896 2316 8 -2.837277783798701 2316 9 -1.2470433862322068 2316 10-11 -4.390039704804934 2316 12-13 -3.694899713584732 2316 14-15 -1.9069931603322132 2316 16-17 -3.166382498548643 2316 18-19 -2.4632159122395265 2316 20-21 -3.2864883938499574 2316 22-23 -3.1070104540759473 2316 24-25 -3.359161980918472 2316 26-27 -4.2121720000602245 2316 28-29 -3.4907215629010366 2316 30-31 -2.701904542220298 2316 32-33 -2.7748660946355628 2316 34-35 -3.8498258871139655 2316 36-37 -1.9405374291358015 2316 38-39 -2.920396288727485 2316 40-41 -2.367121656095943 2316 42-43 -6.51239977684811 2316 44-45 -3.3964022816617643 2316 46-47 -4.378133883640885 2316 48-49 -4.249828845028862 2316 50-51 -3.123413612930701 2316 52-53 -4.466069319031551 2316 54-55 -4.364149507857967 2316 56-57 -4.359100724692386 2316 58-59 -3.1788804211875963 2316 60-61 -3.040406429232302 2316 62-63 -2.125147421428178 2316 64-65 -2.133801795647134 2316 66-67 -2.3869540381655447 2316 68-69 -2.579625451932486 2316 70-71 -2.841690484921795 2316 72-73 -3.371776789321274 2316 74-75 -4.166472123420537 2316 76-77 -3.903036450628939 2316 78-79 -4.0227184005927725 2316 80-81 -3.9400277009932445 2316 82-83 -3.4569973337628284 2316 84-85 -3.2322637368270186 2316 86-87 -3.30440037119973 2316 88-89 -3.1599713106637815 2316 90-91 -2.3783392128310474 2316 92-93 -2.252920327957785 2316 94-95 -1.7474815964708021 2316 96-97 -1.9393580879989187 2316 98-99 -2.044007147502624 2316 100-101 -1.7083958109941868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16757.0 3 4926.0 4 5626.0 5 6566.0 6 7762.0 7 9603.0 8 12212.0 9 14828.0 10 17797.0 11 21662.0 12 25065.0 13 28156.0 14 30298.0 15 34210.0 16 38111.0 17 40576.0 18 37013.0 19 36814.0 20 39210.0 21 42940.0 22 47943.0 23 52909.0 24 59079.0 25 66446.0 26 74513.0 27 83850.0 28 94797.0 29 108301.0 30 122032.0 31 140376.0 32 161830.0 33 186524.0 34 218369.0 35 255061.0 36 268012.0 37 161273.0 38 21642.0 39 132.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.12807673522325 18.608209552500995 13.668851557401606 22.59486215487414 2 15.072991475225587 21.187733001301854 40.46313296556164 23.27614255791092 3 19.346406944725434 26.276306421647135 30.46931104085941 23.90797559276802 4 12.381698655458418 16.70190495399317 38.415297125993106 32.50109926455531 5 13.153063411074495 38.3416248231181 34.24869836158618 14.256613404221227 6 30.106375336864243 36.76618821452102 18.412348869704115 14.715087578910618 7 27.06919958827471 34.011830342917726 21.661732529194452 17.25723753961311 8 25.448712799090366 34.28416056950761 20.707025842891042 19.560100788510976 9 26.94349380533434 16.906752923166994 20.61594463148285 35.53380864001582 10-11 24.51594257068136 26.40931228224897 28.05407465440742 21.02067049266225 12-13 26.74920250094468 24.130725546982692 27.951287149283367 21.16878480278926 14-15 23.442468376661207 25.104394141299412 27.170900909129355 24.282236572910026 16-17 21.848581616019704 28.479034826166526 27.658690360658067 22.0136931971557 18-19 21.597895940184145 27.678022461842673 29.850498980411544 20.873582617561638 20-21 22.88493509960331 26.538495489838205 29.085197911489608 21.49137149906888 22-23 22.37686743561671 26.46173825367083 28.8925608594343 22.268833451278162 24-25 22.565932945747296 26.461897303549048 29.29021897612742 21.681950774576237 26-27 22.666531663102212 26.173332982942405 29.579016890646344 21.581118463309046 28-29 21.92782957345056 26.72952997439737 29.768455630162794 21.574184821989277 30-31 21.925459536709308 26.992011796297422 29.411025788940197 21.67150287805307 32-33 21.819490566602866 26.965593004010657 29.172313242698262 22.04260318668822 34-35 22.51551984975742 26.51453059820737 28.968125619489797 22.00182393254541 36-37 22.60274144618985 26.258814420054094 29.451372683947802 21.68707144980825 38-39 22.69426972949238 26.23508000634402 29.65250640052684 21.41814386363676 40-41 22.25632119402639 26.541915430214036 29.646078816791864 21.555684558967705 42-43 21.854611658690416 27.05438178806508 28.96435556542862 22.12665098781589 44-45 22.196258251583856 27.07033417465614 28.488791292695332 22.244616281064673 46-47 22.327634585475806 27.019284581301655 27.940419394036837 22.7126614391857 48-49 22.635040139978283 26.96131832207721 27.96802097664381 22.4356205613007 50-51 22.653831204837882 27.34416840822737 28.073894100095497 21.928106286839252 52-53 22.448339639982578 27.412339251008632 28.068645781034352 22.07067532797444 54-55 21.748102987185074 27.00006668902521 28.470801321795808 22.781029001993904 56-57 22.296984041184984 27.825375441563803 27.531202489435223 22.34643802781598 58-59 22.4902534018921 27.122876872001694 27.750306277500748 22.636563448605457 60-61 22.482184717901678 26.82651317362979 28.34128064473876 22.350021463729774 62-63 22.982955516953773 26.74031144565988 28.02046860398147 22.256264433404876 64-65 22.48994502119176 27.03216820501725 28.204341123607662 22.273545650183326 66-67 22.006039765233155 27.298861733579933 28.012652170225476 22.682446330961437 68-69 21.888544090227352 27.61089685686314 28.04511908561043 22.455439967299075 70-71 22.089765797945038 27.44993293995044 27.578372931155933 22.88192833094859 72-73 22.26790871753211 27.08264652218157 27.460104404312986 23.189340355973336 74-75 22.100416884690123 27.62468509423156 27.443140094479944 22.83175792659837 76-77 22.53131612688475 26.941814430383122 27.684146248820625 22.8427231939115 78-79 22.174997066817227 27.08654638458887 27.940696939690092 22.79775960890381 80-81 22.05791729392489 27.676121352140644 27.54546993915692 22.72049141477755 82-83 22.142149451676012 27.52103593397923 27.335136533039954 23.0016780813048 84-85 22.200402987081844 27.080148364787554 27.28262734592739 23.43682130220321 86-87 22.39296762553781 27.033742658505926 27.63685055122079 22.936439164735475 88-89 22.571797958872104 26.64937705209495 27.570841361344574 23.207983627688378 90-91 21.972483986736076 26.98403803803533 27.90509659356927 23.13838138165933 92-93 21.981146639971232 27.409772004845212 27.66045711872106 22.948624236462493 94-95 22.00460867351505 27.26704423433526 27.244969302894155 23.483377789255538 96-97 21.94419002684291 26.960873312022194 27.048383943906572 24.046552717228323 98-99 22.066269452195993 26.94599314253889 26.867381807422536 24.12035559784259 100-101 22.41252981163629 26.912919371622053 27.14938199291115 23.525168823830516 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4029.0 1 2768.0 2 1386.5 3 1130.5 4 959.5 5 990.0 6 1351.0 7 2925.5 8 4044.5 9 3601.0 10 3154.0 11 3017.5 12 3135.0 13 3286.0 14 3440.0 15 3690.5 16 3929.0 17 4164.0 18 4526.5 19 4964.0 20 5519.5 21 6369.0 22 7531.0 23 8885.0 24 10738.0 25 13177.0 26 16575.0 27 20530.5 28 24177.0 29 28091.5 30 32133.5 31 35972.5 32 39612.5 33 43804.0 34 48308.0 35 52509.5 36 57707.5 37 62379.0 38 64835.0 39 66742.0 40 70013.0 41 73696.0 42 77458.5 43 82351.0 44 86894.5 45 90474.5 46 97708.5 47 118764.0 48 136412.5 49 126321.5 50 108019.5 51 97965.5 52 90977.0 53 86418.5 54 81589.0 55 74816.5 56 67530.0 57 60107.0 58 52970.5 59 45520.0 60 37721.5 61 30577.5 62 24353.0 63 18795.5 64 14330.0 65 10590.0 66 7714.0 67 5807.0 68 4197.0 69 2941.0 70 1946.5 71 1291.5 72 904.5 73 636.5 74 436.0 75 288.5 76 189.5 77 122.0 78 73.5 79 56.5 80 34.5 81 20.5 82 12.0 83 12.0 84 15.0 85 11.5 86 8.5 87 8.0 88 5.5 89 7.0 90 5.0 91 4.0 92 5.5 93 4.5 94 3.0 95 2.0 96 3.0 97 2.0 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 53.02309367385194 2 0.08876991201289824 3 0.12220323682401153 4 0.19770779274115086 5 0.12401565466267626 6 0.1503535564458255 7 0.1587215281690222 8 0.15567512371679854 9 0.14402937505133576 10-11 0.13087970520059802 12-13 0.14343166278539315 14-15 0.14944734752649314 16-17 0.14499342709317872 18-19 0.15197316387612164 20-21 0.18037413702881475 22-23 0.19845975333378837 24-25 0.22514471385200102 26-27 0.2172973302313995 28-29 0.2157934090461245 30-31 0.23015778446958435 32-33 0.22128850568462927 34-35 0.2084666135281181 36-37 0.19518197639152238 38-39 0.1911715198974557 40-41 0.19294537565444672 42-43 0.1854257697280718 44-45 0.20179537339856496 46-47 0.22632085734304946 48-49 0.22992641197954206 50-51 0.23991399113303497 52-53 0.2672159449580271 54-55 0.25406627510728935 56-57 0.23011922238791063 58-59 0.18826008273109002 60-61 0.1979777273128669 62-63 0.20274014439957103 64-65 0.19092086636657654 66-67 0.20077347823421143 68-69 0.1895326314263227 70-71 0.2021424321336284 72-73 0.22288883207408858 74-75 0.25198392269690856 76-77 0.2535264059638573 78-79 0.24835908701957912 80-81 0.25323719035130443 82-83 0.24440647364802306 84-85 0.2138267428807649 86-87 0.2264751056697443 88-89 0.22851889599845135 90-91 0.21596693841365622 92-93 0.21257347522636907 94-95 0.25360353012720477 96-97 0.27955581109361677 98-99 0.2975450221944061 100-101 0.2935152846595026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2593221.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.90757512345458 #Duplication Level Percentage of deduplicated Percentage of total 1 79.66107348664035 52.50268185235784 2 11.96813107030745 15.775809952072784 3 3.9442641133403913 7.798706500701832 4 1.7178263356075418 4.52871073052411 5 0.8862569822898115 2.9205524319475944 6 0.5213290114360916 2.061571859115631 7 0.3101244091643916 1.4307683456233373 8 0.20416713964231215 1.0764928874973223 9 0.14907162155121664 0.88424541865458 >10 0.5999045552074483 6.463341029722201 >50 0.024864765015755932 1.1097727940661388 >100 0.010780257815207491 1.365225542136966 >500 0.0012702662927021728 0.574232247339523 >1k 8.691295686898839E-4 1.2825930214505 >5k 6.68561206684526E-5 0.22529538678976244 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5113 0.19716792359771884 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4997 0.19269472212356756 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3807 0.14680584493184345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3613 0.1393248010871422 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2670 0.10296075806882639 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.8562081673717744E-5 0.0 8 0.0 0.0 0.0 3.8562081673717744E-5 0.0 9 0.0 0.0 0.0 3.8562081673717744E-5 0.0 10-11 0.0 0.0 0.0 7.712416334743549E-5 0.0 12-13 0.0 0.0 0.0 7.712416334743549E-5 0.0 14-15 0.0 0.0 0.0 7.712416334743549E-5 0.0 16-17 0.0 0.0 0.0 7.712416334743549E-5 0.0 18-19 0.0 0.0 0.0 9.640520418429436E-5 0.0 20-21 0.0 0.0 0.0 2.506535308791653E-4 0.0 22-23 0.0 0.0 0.0 6.941174701269193E-4 0.0 24-25 0.0 0.0 0.0 0.0013882349402538387 0.0 26-27 0.0 0.0 0.0 0.0021787576145650524 0.0 28-29 0.0 0.0 0.0 0.005090194780930742 0.0 30-31 0.0 0.0 0.0 0.014113721892580694 0.0 32-33 0.0 0.0 0.0 0.031717312176632845 0.0 34-35 0.0 0.0 0.0 0.058826455593256416 0.0 36-37 0.0 0.0 0.0 0.09812121681877481 0.0 38-39 3.8562081673717744E-5 0.0 0.0 0.15208885012114276 0.0 40-41 7.712416334743549E-5 0.0 0.0 0.21637184027123024 0.0 42-43 7.712416334743549E-5 0.0 0.0 0.282120189524919 0.0 44-45 7.712416334743549E-5 0.0 0.0 0.35912866662733334 0.0 46-47 7.712416334743549E-5 0.0 0.0 0.4533551131970627 0.0 48-49 7.712416334743549E-5 0.0 0.0 0.5576077010019586 0.0 50-51 7.712416334743549E-5 0.0 0.0 0.6633256479104557 0.0 52-53 7.712416334743549E-5 0.0 0.0 0.7653994781007867 0.0 54-55 7.712416334743549E-5 0.0 0.0 0.8798902985900545 0.0 56-57 7.712416334743549E-5 0.0 0.0 1.0137007219978553 0.0 58-59 7.712416334743549E-5 0.0 0.0 1.1695108130005116 0.0 60-61 7.712416334743549E-5 0.0 0.0 1.3565947522405533 0.0 62-63 7.712416334743549E-5 0.0 0.0 1.5363518959625886 0.0 64-65 7.712416334743549E-5 0.0 0.0 1.7087436820849438 0.0 66-67 7.712416334743549E-5 0.0 0.0 1.9096328465641763 0.0 68-69 7.712416334743549E-5 0.0 0.0 2.14071612099393 0.0 70-71 7.712416334743549E-5 0.0 0.0 2.39484023922373 0.0 72-73 7.712416334743549E-5 0.0 0.0 2.6663365752475396 0.0 74-75 7.712416334743549E-5 0.0 0.0 2.940821472601063 0.0 76-77 7.712416334743549E-5 0.0 0.0 3.227395582559296 0.0 78-79 7.712416334743549E-5 0.0 0.0 3.5541321005807065 0.0 80-81 7.712416334743549E-5 0.0 0.0 3.917888217008886 0.0 82-83 7.712416334743549E-5 0.0 0.0 4.290629298467041 0.0 84-85 7.712416334743549E-5 3.8562081673717744E-5 0.0 4.697574946369785 0.0 86-87 7.712416334743549E-5 3.8562081673717744E-5 0.0 5.127561438072575 0.0 88-89 7.712416334743549E-5 3.8562081673717744E-5 0.0 5.55874335430725 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10360 0.0 33.188736 1 TACATGG 12015 0.0 26.65664 2 ACATGGG 11880 0.0 25.847181 3 CATGGGA 8430 0.0 22.636606 4 ATAACGC 210 3.6565325E-8 22.49557 3 GAGTACT 9255 0.0 21.952974 12-13 CATGGGG 4315 0.0 20.688953 4 AGTACTT 9870 0.0 20.441633 12-13 ATGGGAT 3755 0.0 20.235435 5 GTACTTT 10235 0.0 19.808886 14-15 GCTTATA 1850 0.0 17.933563 1 GTATACA 1100 0.0 17.36542 1 GTGTAGC 1075 0.0 16.834044 1 ACTTTTT 12755 0.0 16.732637 16-17 GTATATG 1040 1.8189894E-12 16.433874 1 GTATCAA 31460 0.0 16.36197 1 GTCCTAT 615 1.05053E-6 16.347424 1 TACTTTT 12305 0.0 16.342281 14-15 ATGGGAG 3585 0.0 15.797512 5 TATAACG 240 6.4488406E-5 15.746288 2 >>END_MODULE