FastQCFastQC Report
Fri 27 May 2016
SRR939604_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939604_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2593221
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121360.4679894231922385No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA111580.4302757073153426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94270.36352474393813716No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA93260.35962997368909166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67100.2587515680306461No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT63450.2446764082197391No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60760.23430320824950904No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA45100.17391498834846703No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG43340.1671280619738927No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT40930.1578346002905267No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC35640.13743525908513002No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33700.12995421524042877No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG29660.11437513424424682No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26520.10226664059869947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG121900.026.9345251
TACATGG122300.026.224212
ACATGGG123050.024.6806873
CATGGGA86000.022.7015134
GAGTACT92450.021.6148512-13
AGTACTT95150.020.72907812-13
GTACTTT102350.019.73132714-15
CATGGGG41700.018.7726254
ATGGGAG35750.018.5992765
ATGGGAT37800.017.8400975
CCTATAA14750.016.95071
ACTTTTT121600.016.89842416-17
GCTTATA22750.016.796061
TACTTTT118250.016.77931614-15
GTATCAA306400.015.79655551
TTATAGG14700.015.7192453
ATAGGAG13600.015.60366255
AGTACAT91700.015.4816462
CTATAAG15050.015.3560512
CTTATAG14850.015.2452582
Produced by FastQC (version 0.11.5)