##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939604_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2593221 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.530678256885935 31.0 31.0 34.0 30.0 34.0 2 31.73214585259027 31.0 31.0 34.0 30.0 34.0 3 31.783931257690725 31.0 31.0 34.0 30.0 34.0 4 35.565915130256926 37.0 35.0 37.0 33.0 37.0 5 35.329049857301015 37.0 35.0 37.0 33.0 37.0 6 35.33909875016437 37.0 35.0 37.0 32.0 37.0 7 35.251922608987044 37.0 35.0 37.0 32.0 37.0 8 35.219801551815294 37.0 35.0 37.0 32.0 37.0 9 36.82311881632919 39.0 37.0 39.0 32.0 39.0 10-11 36.7019205073536 39.0 35.0 39.0 32.0 39.0 12-13 36.637330177412565 39.0 35.0 39.0 32.0 39.0 14-15 37.69159377469178 39.0 36.5 41.0 32.0 41.0 16-17 37.65073628510643 39.0 36.0 41.0 32.0 41.0 18-19 37.65543256822307 39.0 36.5 41.0 32.0 41.0 20-21 37.60665731921807 39.0 36.0 41.0 32.0 41.0 22-23 37.47288140887336 39.0 36.0 41.0 32.0 41.0 24-25 37.33049805627827 39.0 36.0 41.0 31.5 41.0 26-27 37.023311356802985 39.0 36.0 40.0 31.0 41.0 28-29 36.8279460177131 39.0 36.0 40.0 30.5 41.0 30-31 36.596302629047045 38.0 35.0 40.0 30.0 41.0 32-33 36.432555111963076 38.0 35.0 40.0 30.0 41.0 34-35 36.400650195259104 38.0 35.0 40.0 30.0 41.0 36-37 36.29332478797603 38.0 35.0 40.0 30.0 41.0 38-39 36.31913361799862 38.0 35.0 40.0 30.0 41.0 40-41 36.20099154680608 38.0 35.0 40.0 30.0 41.0 42-43 36.034764680680894 38.0 35.0 40.0 29.5 41.0 44-45 35.81467680540918 38.0 35.0 40.0 28.5 41.0 46-47 35.490866763765986 38.0 34.5 40.0 27.5 41.0 48-49 35.1804659147832 38.0 34.0 40.0 26.5 41.0 50-51 34.93493439240234 38.0 34.0 40.0 26.0 41.0 52-53 34.679599810428805 38.0 33.5 40.0 25.5 41.0 54-55 34.41369941088708 38.0 33.0 40.0 24.5 41.0 56-57 33.63554108963332 37.5 32.5 40.0 21.5 41.0 58-59 30.604544888383984 35.5 27.0 39.0 8.0 40.5 60-61 30.326841214073156 34.5 26.0 39.0 10.5 40.0 62-63 32.09823169718277 35.5 30.5 39.0 18.5 40.0 64-65 32.115921088098546 36.0 31.0 39.0 15.5 40.0 66-67 27.27968788622335 32.0 18.5 37.0 2.0 39.5 68-69 19.877623619429272 20.5 8.0 32.5 2.0 37.0 70-71 17.92508004524103 16.0 7.5 29.0 2.0 35.0 72-73 19.65404298360996 20.0 7.5 31.0 2.0 35.5 74-75 21.07410494516279 24.0 9.0 32.5 2.0 36.0 76-77 22.82622113579984 27.5 11.5 33.0 2.0 35.5 78-79 24.97884329951053 30.5 13.0 34.5 2.0 36.5 80-81 26.586903700070298 32.0 22.5 35.0 2.0 36.5 82-83 27.742958660291585 33.0 26.5 35.0 2.0 36.0 84-85 27.807866356164787 34.0 27.0 35.0 2.0 36.0 86-87 27.532202808784902 34.0 27.0 35.0 2.0 36.0 88-89 27.091594006064273 33.0 26.0 35.0 2.0 35.0 90-91 25.268975725555208 32.5 16.0 34.5 2.0 35.0 92-93 23.68992056596796 31.5 2.0 34.0 2.0 35.0 94-95 20.008495997834352 28.0 2.0 34.0 2.0 35.0 96-97 17.311021698497736 18.5 2.0 33.0 2.0 35.0 98-99 17.604103738169634 22.0 2.0 33.0 2.0 35.0 100-101 16.84478974217778 18.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.29780316033343723 1101 2 0.28241159001591853 1101 3 0.2847881401165644 1101 4 0.27561154942404187 1101 5 0.3777144069437526 1101 6 0.2892206539286093 1101 7 0.35440361607333415 1101 8 0.45041142253007393 1101 9 0.5278748729038796 1101 10-11 0.33388830857333573 1101 12-13 0.36957606377693253 1101 14-15 0.48399011748888654 1101 16-17 0.5058925327965156 1101 18-19 0.4776024142844548 1101 20-21 0.506174889767685 1101 22-23 0.46229531964627313 1101 24-25 0.4453596636377668 1101 26-27 0.5429726414123124 1101 28-29 0.5642273214614022 1101 30-31 0.2953769609893939 1101 32-33 0.3680146656112697 1101 34-35 0.17827500176155553 1101 36-37 0.24952714437095125 1101 38-39 0.1441727652094258 1101 40-41 0.31937984844236667 1101 42-43 0.4978766751930621 1101 44-45 0.33123497517313893 1101 46-47 0.23366919054152646 1101 48-49 0.1468469608959282 1101 50-51 0.23944974534908425 1101 52-53 0.19777505313457056 1101 54-55 0.3264967737361033 1101 56-57 -3.223183942783784 1101 58-59 -9.967538309359423 1101 60-61 -11.643760778028977 1101 62-63 -3.6501942938878926 1101 64-65 -0.8353302643159459 1101 66-67 -1.8858928302865259 1101 68-69 -4.747147189192061 1101 70-71 -4.384157466943735 1101 72-73 -3.3379507772269275 1101 74-75 -5.951031092325739 1101 76-77 -4.490790470794753 1101 78-79 1.6296884838778638 1101 80-81 2.2148618885136635 1101 82-83 0.8914966207626982 1101 84-85 0.601415494097072 1101 86-87 0.43989737238044313 1101 88-89 0.39442069412621805 1101 90-91 1.6612397328106177 1101 92-93 0.9938971624673094 1101 94-95 -8.34951675533518 1101 96-97 -9.21620098588926 1101 98-99 -8.276739639969438 1101 100-101 -9.718895750270223 1102 1 0.2560764249035188 1102 2 0.3040412989115424 1102 3 0.3175689646810973 1102 4 0.2518162687233314 1102 5 0.2970532046767502 1102 6 0.300807113314697 1102 7 0.34884694583969633 1102 8 0.3109261808678667 1102 9 0.3420388395725311 1102 10-11 0.3889404011956472 1102 12-13 0.3851032198120521 1102 14-15 0.46658503852013666 1102 16-17 0.4211060927315202 1102 18-19 0.4673253920107072 1102 20-21 0.3964865413738039 1102 22-23 0.39498950895329443 1102 24-25 0.40636884363102865 1102 26-27 0.37777239568772814 1102 28-29 0.33067594471012285 1102 30-31 0.4153057949865868 1102 32-33 0.27283230427721605 1102 34-35 0.3211058258306778 1102 36-37 0.1874160431680849 1102 38-39 0.31890722411991845 1102 40-41 0.21174653294388435 1102 42-43 0.45591368993654413 1102 44-45 0.37513502322815384 1102 46-47 0.35492882631427136 1102 48-49 0.358973915298165 1102 50-51 0.3753070194905348 1102 52-53 0.3504899754538897 1102 54-55 0.21037301722142843 1102 56-57 -3.4145010959455036 1102 58-59 -10.067211584828577 1102 60-61 -7.97355593735363 1102 62-63 -2.2258914631009787 1102 64-65 -0.4645214539003959 1102 66-67 -1.7335547310851318 1102 68-69 -4.892715476848364 1102 70-71 -4.710800873994447 1102 72-73 -3.4229112309256777 1102 74-75 -4.210997552900814 1102 76-77 -4.14632243233093 1102 78-79 1.8581104836598819 1102 80-81 2.3495784754189195 1102 82-83 0.9482111318896607 1102 84-85 0.5841172384444135 1102 86-87 0.4730042402836645 1102 88-89 0.43075858030024605 1102 90-91 1.69656047523576 1102 92-93 1.479402322188296 1102 94-95 -8.988688338970714 1102 96-97 -9.94251036107504 1102 98-99 -9.219099326769669 1102 100-101 -10.045678801713855 1103 1 0.1819506453438926 1103 2 0.19105288094030826 1103 3 0.2123403468880447 1103 4 0.1973067549139884 1103 5 0.21436817858536727 1103 6 0.2592438576191398 1103 7 0.2877668580199213 1103 8 0.3077641915589737 1103 9 0.2440251256868251 1103 10-11 0.19436470436479425 1103 12-13 0.24246568322048034 1103 14-15 0.37675272338713484 1103 16-17 0.3577927256703006 1103 18-19 0.34089553646787607 1103 20-21 0.278715365988802 1103 22-23 0.26138850029858673 1103 24-25 0.39214983510360213 1103 26-27 0.3785996834565921 1103 28-29 0.2289414245370267 1103 30-31 0.3341957975320824 1103 32-33 0.34807571779382585 1103 34-35 0.29000895361482293 1103 36-37 0.25289109725998316 1103 38-39 0.2467740944266481 1103 40-41 0.18988704458955397 1103 42-43 0.13967299692394164 1103 44-45 0.16277065069409957 1103 46-47 0.04534819757557074 1103 48-49 0.12226620243065867 1103 50-51 -0.016539025437552368 1103 52-53 0.021478027509381548 1103 54-55 -0.08026948309675674 1103 56-57 0.3373523468443622 1103 58-59 -8.186810350260675 1103 60-61 -7.753952796841983 1103 62-63 -2.3670911894596394 1103 64-65 -0.4041453561531618 1103 66-67 -1.890110986653415 1103 68-69 -3.9256876672518253 1103 70-71 -4.146976418095248 1103 72-73 -3.270274648896912 1103 74-75 -4.266513335005943 1103 76-77 -0.9728127435184071 1103 78-79 2.936971292492771 1103 80-81 2.4614313613804804 1103 82-83 0.9367066613538313 1103 84-85 0.43430854874096525 1103 86-87 0.4480961169541473 1103 88-89 0.3535304716073604 1103 90-91 1.6694528800976691 1103 92-93 1.1991645565016462 1103 94-95 -7.2101011714767616 1103 96-97 -9.007277447749344 1103 98-99 -8.291667975745105 1103 100-101 -9.763917712875877 1104 1 0.2837228135039602 1104 2 0.21099756982420104 1104 3 0.19343907544606154 1104 4 0.17332385497001468 1104 5 0.23501342736049224 1104 6 0.20627065317854942 1104 7 0.26056163950532607 1104 8 0.2171034913559282 1104 9 0.3115656366187949 1104 10-11 0.29378247457744777 1104 12-13 0.4122735930669279 1104 14-15 0.5165887883759126 1104 16-17 0.41037753902204344 1104 18-19 0.40949302498636087 1104 20-21 0.46809962837046726 1104 22-23 0.4415137532131297 1104 24-25 0.5227082632687399 1104 26-27 0.4921972255019611 1104 28-29 0.5249681594326319 1104 30-31 0.46598841296281535 1104 32-33 0.5859813177766995 1104 34-35 0.40844061583874947 1104 36-37 0.35203730773483954 1104 38-39 0.36352358502910675 1104 40-41 0.4077486097870846 1104 42-43 0.4614110506149771 1104 44-45 0.567068145992657 1104 46-47 0.43765352921031564 1104 48-49 0.4828878993756973 1104 50-51 0.4908235621235093 1104 52-53 0.39937013871684 1104 54-55 0.5100269488009843 1104 56-57 1.0045109612830672 1104 58-59 -7.9245742824171295 1104 60-61 -7.314481214266056 1104 62-63 -1.5540406208256812 1104 64-65 0.23930512969254636 1104 66-67 -1.1328033176779577 1104 68-69 -3.112333280009782 1104 70-71 -3.3952872879785225 1104 72-73 -2.4962005034536574 1104 74-75 -3.4605767037027064 1104 76-77 -0.05872096988717601 1104 78-79 4.129685783648341 1104 80-81 3.6678373164250324 1104 82-83 2.0128451603885615 1104 84-85 1.527717604776477 1104 86-87 1.6880273884318093 1104 88-89 1.50798094674769 1104 90-91 2.731893563386219 1104 92-93 2.6842656687903492 1104 94-95 -5.867685324663572 1104 96-97 -7.420597934629903 1104 98-99 -5.1318616003360695 1104 100-101 -4.859906836212527 1105 1 0.2673955602437772 1105 2 0.3083063589297552 1105 3 0.2911687227131843 1105 4 0.2268835494384689 1105 5 0.2673429479642735 1105 6 0.32879664190480895 1105 7 0.3265147460809743 1105 8 0.2829547074288712 1105 9 0.31557995447377607 1105 10-11 0.32210586495592963 1105 12-13 0.25759030387914805 1105 14-15 0.3880058431385578 1105 16-17 0.4163485604113575 1105 18-19 0.4154657258894545 1105 20-21 0.41381291437416223 1105 22-23 0.5054879726765975 1105 24-25 0.547834419246584 1105 26-27 0.3159654246083221 1105 28-29 0.4273109411973124 1105 30-31 0.4944377668748601 1105 32-33 0.42996498450518317 1105 34-35 0.5076364086567295 1105 36-37 0.4245980414024402 1105 38-39 0.4513112804912751 1105 40-41 0.32769353573102933 1105 42-43 0.31940613103919446 1105 44-45 0.4007752877751187 1105 46-47 0.2869623043199212 1105 48-49 0.1526486036518122 1105 50-51 0.06737908929839165 1105 52-53 0.2107115453796169 1105 54-55 0.19830618196298389 1105 56-57 0.7757900859764604 1105 58-59 -7.9054481194413135 1105 60-61 -7.364675792935532 1105 62-63 -1.898491052425733 1105 64-65 -0.21535863514753473 1105 66-67 -1.6355146827377673 1105 68-69 -3.3684351985043754 1105 70-71 -3.805357722587704 1105 72-73 -2.8202979572407862 1105 74-75 -3.621424648222767 1105 76-77 -0.24003364986632292 1105 78-79 3.8147764598975726 1105 80-81 3.1986289622435713 1105 82-83 1.6372125260362083 1105 84-85 1.2800271111743768 1105 86-87 1.248895976975426 1105 88-89 1.2586760905035739 1105 90-91 2.7388724330034613 1105 92-93 2.5654503558234545 1105 94-95 -5.850150081116581 1105 96-97 -7.608502776178275 1105 98-99 -5.423593773871831 1105 100-101 -4.5708926758119635 1106 1 0.14985087179265477 1106 2 0.15641264715747738 1106 3 0.1708990893602298 1106 4 0.15764608781635303 1106 5 0.13249126237140985 1106 6 0.17786217993246822 1106 7 0.2770303891840342 1106 8 0.18536466638308724 1106 9 0.1489733524682677 1106 10-11 0.23805343749928198 1106 12-13 0.15633954483344326 1106 14-15 0.24129931550339023 1106 16-17 0.266508167661236 1106 18-19 0.30685639372199347 1106 20-21 0.2328585466298918 1106 22-23 0.26114088103494026 1106 24-25 0.23449575354424468 1106 26-27 0.19032587325770578 1106 28-29 0.33499807831237405 1106 30-31 0.32468041300393935 1106 32-33 0.3009997043505592 1106 34-35 0.313510100980416 1106 36-37 0.41821759554554916 1106 38-39 0.407252169725588 1106 40-41 0.43369372766335346 1106 42-43 0.3804931270469183 1106 44-45 0.46152612761636647 1106 46-47 0.34999361057047906 1106 48-49 0.35853845508547266 1106 50-51 0.42454649204890416 1106 52-53 0.4814355352876518 1106 54-55 0.5097996612747195 1106 56-57 1.0292175814777877 1106 58-59 -8.067265062398558 1106 60-61 -7.28242901216111 1106 62-63 -1.5946154882812742 1106 64-65 0.13838467004533328 1106 66-67 0.9066710970532377 1106 68-69 -2.4580879863215337 1106 70-71 -2.1567923481418596 1106 72-73 -0.812615652756751 1106 74-75 -0.6343489158148827 1106 76-77 2.5915247898912916 1106 78-79 4.862211067329362 1106 80-81 3.7257110539545515 1106 82-83 1.9778587147034976 1106 84-85 1.7560113375765525 1106 86-87 1.4894428633126324 1106 88-89 1.3364050477247673 1106 90-91 2.6837354080068145 1106 92-93 2.485191269138543 1106 94-95 -5.738560374785408 1106 96-97 -7.448240100715672 1106 98-99 -5.271092894181965 1106 100-101 -4.709169282706398 1107 1 0.01637670735904706 1107 2 0.08004421746945312 1107 3 0.04596094894236913 1107 4 0.007460063654349369 1107 5 -0.03141409359808023 1107 6 0.03004204379751485 1107 7 0.019504527823421824 1107 8 0.05786186292410633 1107 9 -0.03070649610639009 1107 10-11 -0.08190619322327564 1107 12-13 0.049302457271650724 1107 14-15 0.09277070267111043 1107 16-17 0.048046715521337546 1107 18-19 0.06562011152475833 1107 20-21 0.07836834537384618 1107 22-23 0.0385372634094594 1107 24-25 0.11754211957799754 1107 26-27 0.08378140203514306 1107 28-29 0.006514722362567227 1107 30-31 -0.09424750226764189 1107 32-33 -0.12533766591052142 1107 34-35 -0.13330275772870692 1107 36-37 -0.05071892222998997 1107 38-39 0.09834795322223755 1107 40-41 0.046449618916007296 1107 42-43 -0.08726583159006651 1107 44-45 -0.11114408961081779 1107 46-47 -0.1314463874298042 1107 48-49 -0.1954351438697799 1107 50-51 -0.17508692427799133 1107 52-53 -0.12068157151609427 1107 54-55 -0.13039981310829063 1107 56-57 0.4628428149220909 1107 58-59 -8.38914430132559 1107 60-61 -7.731514686984337 1107 62-63 -2.3084143035024134 1107 64-65 -0.5339658983448388 1107 66-67 0.6065540657707054 1107 68-69 -2.950349721559597 1107 70-71 -2.7220417423101875 1107 72-73 -1.3551438661775599 1107 74-75 -1.0651485524621371 1107 76-77 2.084340303011377 1107 78-79 4.047714491976876 1107 80-81 3.04217629970562 1107 82-83 1.40480723503293 1107 84-85 0.8440019229536766 1107 86-87 0.7416314493455154 1107 88-89 0.6508962382022894 1107 90-91 2.15650682099535 1107 92-93 1.7435498582002538 1107 94-95 -6.720063927606244 1107 96-97 -8.177198003757606 1107 98-99 -6.077068204064901 1107 100-101 -5.3285359389756035 1108 1 -0.06986453372824997 1108 2 -0.1422159456323051 1108 3 -0.051334468766732755 1108 4 -0.031133370047960796 1108 5 -0.010464645968049524 1108 6 0.00294930163289564 1108 7 0.03561555754989598 1108 8 -0.07675704063544941 1108 9 -0.06705406144015313 1108 10-11 -0.157533125710124 1108 12-13 -0.13629578148375288 1108 14-15 -0.2388820958800224 1108 16-17 -0.20051390598154484 1108 18-19 -0.10510798148380474 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3.402933712739202 2313 60-61 3.2980483600500854 2313 62-63 0.3938480369195254 2313 64-65 -0.2449293534943422 2313 66-67 -2.305927172740521 2313 68-69 -4.974197471024157 2313 70-71 -3.6469173683958243 2313 72-73 -2.732285205537057 2313 74-75 -3.8286972986044354 2313 76-77 0.21074467422745613 2313 78-79 2.9058186119154925 2313 80-81 2.2759849240879646 2313 82-83 0.8355566827284271 2313 84-85 0.48384204977291034 2313 86-87 0.5429443319534073 2313 88-89 0.449488072527803 2313 90-91 2.09658726351352 2313 92-93 3.260151035325574 2313 94-95 5.774650813443049 2313 96-97 1.6242885807238991 2313 98-99 3.1021145539161132 2313 100-101 4.419575263952032 2314 1 -0.3948349030911409 2314 2 -0.3605274364905249 2314 3 -0.435465319603189 2314 4 -0.2590675317438027 2314 5 -0.34288882068194226 2314 6 -0.45843129175704433 2314 7 -0.3063144655717167 2314 8 -0.4969379332462438 2314 9 -0.4379140299636717 2314 10-11 -0.47152631552106783 2314 12-13 -0.4743114031442346 2314 14-15 -0.6591427726243424 2314 16-17 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2314 82-83 0.5585975599844204 2314 84-85 0.1416257886612975 2314 86-87 0.18643955059397044 2314 88-89 0.21846086591920155 2314 90-91 1.8197263494433038 2314 92-93 3.3453192808135874 2314 94-95 5.953054445480401 2314 96-97 3.4053937930791136 2314 98-99 5.751858088979414 2314 100-101 6.288725394148672 2315 1 -0.48414347602416896 2315 2 -0.42868636212761757 2315 3 -0.45812610181637226 2315 4 -0.3167247732984251 2315 5 -0.3987075298849305 2315 6 -0.49717313235477434 2315 7 -0.5453290049683517 2315 8 -0.6813140964433728 2315 9 -0.6936508046960839 2315 10-11 -0.5812457637230466 2315 12-13 -0.6163594471573504 2315 14-15 -0.6702264524580173 2315 16-17 -0.7195751512293853 2315 18-19 -0.6448260379855171 2315 20-21 -0.785768061300189 2315 22-23 -0.8463844947251147 2315 24-25 -0.6879746986291764 2315 26-27 -0.6282283424818615 2315 28-29 -0.6235526810296008 2315 30-31 -0.5144821730564786 2315 32-33 -0.5706086131265806 2315 34-35 -0.6604620303050481 2315 36-37 -0.5620150375038975 2315 38-39 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3.0808819809116024 2316 62-63 0.45202271001167915 2316 64-65 0.02352655891295541 2316 66-67 -2.1522004622448314 2316 68-69 -3.130196749326565 2316 70-71 -3.55353198299483 2316 72-73 -0.6475075674706261 2316 74-75 5.551697585807009 2316 76-77 5.669416795205354 2316 78-79 4.803321683028322 2316 80-81 2.9955860859319934 2316 82-83 1.314466685479342 2316 84-85 0.8927061222634229 2316 86-87 0.6157310424713671 2316 88-89 0.7125675606125519 2316 90-91 2.253742984247733 2316 92-93 3.8062532557707733 2316 94-95 6.379575508531385 2316 96-97 3.6738791175755843 2316 98-99 5.957035796438802 2316 100-101 6.12528413693437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 19.0 4 44.0 5 112.0 6 276.0 7 621.0 8 1389.0 9 2474.0 10 4056.0 11 6308.0 12 9058.0 13 12368.0 14 15308.0 15 20193.0 16 26790.0 17 35941.0 18 38172.0 19 36108.0 20 37651.0 21 38701.0 22 38953.0 23 39664.0 24 42065.0 25 44751.0 26 55153.0 27 77417.0 28 110177.0 29 146987.0 30 179329.0 31 199702.0 32 210066.0 33 211235.0 34 217729.0 35 233199.0 36 237648.0 37 184122.0 38 74276.0 39 5154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33608479649458 19.49058073872929 14.267403647360139 22.90593081741599 2 15.22916191805114 21.755196287035826 39.28197462185594 23.7336671730571 3 19.21928661608858 26.240871380655236 30.735865631042202 23.803976372213985 4 12.678055591868182 17.305196896060924 37.82188251599073 32.19486499608016 5 13.06040974424652 38.65574591581231 34.30794834998316 13.975895989958012 6 30.425289630154932 37.13432059974834 17.842829438755896 14.59756033134083 7 26.94309509293655 34.05968870373948 21.47503047368504 17.52218572963893 8 25.507313105979012 34.614057189880846 20.490463404391683 19.38816629974846 9 26.400835100440727 16.694103587777516 20.638696046345455 36.266365265436306 10-11 24.41224639164961 26.524561539490847 28.008025540437938 21.055166528421605 12-13 26.369310598672463 24.130184045247205 28.12195721074293 21.3785481453374 14-15 23.088533272353605 25.216184642093403 27.114178693099273 24.58110339245372 16-17 21.633798906378942 28.651599170143683 27.62436276135461 22.090239162122767 18-19 21.4635060548486 27.768970592828733 29.74568980310511 21.021833549217554 20-21 22.550083467625782 26.735033381266 29.000131496698504 21.71475165440971 22-23 22.142019519354502 26.69213306540399 28.812044943334946 22.35380247190656 24-25 22.367359325872464 26.674613033957613 29.154928518364343 21.803099121805587 26-27 22.58940871716314 26.33130464374855 29.42819104066666 21.65109559842165 28-29 21.80879431836455 26.8740284675634 29.64821029794497 21.66896691612708 30-31 21.90053474677861 27.067446198010757 29.266438792385863 21.765580262824773 32-33 21.78614517129523 27.05869445291249 28.968256493288003 22.18690388250427 34-35 22.437040476265764 26.60735453926347 28.805433033032106 22.15017195143866 36-37 22.541503228747217 26.41958931555036 29.233352489807764 21.805554965894657 38-39 22.7321933451634 26.314075128379887 29.426491371744767 21.527240154711947 40-41 22.19481742499971 26.666274281259287 29.454860883291715 21.684047410449285 42-43 21.825785013025413 27.135182744660003 28.87454207452903 22.16449016778555 44-45 22.12641992272687 27.19383127284517 28.35317912158746 22.326569682840503 46-47 22.18301528433746 27.159929184358035 27.8814783595071 22.775577171797405 48-49 22.50542842439367 26.99010871899604 27.97740370709335 22.527059149516937 50-51 22.472494809221903 27.340278296324982 28.149289112785514 22.037937781667605 52-53 22.40236682965791 27.39869639158799 28.007400683540713 22.191536095213383 54-55 21.714053455106075 26.947273308096047 28.475573433253647 22.86309980354423 56-57 22.14968724314771 27.74604761004372 27.688339050809002 22.415926095999566 58-59 21.218681725793314 26.89519295217961 29.35143338456771 22.53469193745937 60-61 21.56323923030673 26.79320780588513 29.199176356701617 22.444376607106516 62-63 22.877710881123736 26.76583867997744 28.069857460315927 22.286592978582895 64-65 22.39027362421452 27.011883207906973 28.22519286351953 22.372650304358977 66-67 21.609500676152685 27.040610872472808 28.710780123099163 22.639108328275345 68-69 20.845401796924477 27.057883615264224 29.708477510016696 22.388237077794606 70-71 21.619734239282547 27.038612716580456 28.536985710851297 22.8046673332857 72-73 21.08390560303122 26.775626110456447 28.869207640313306 23.271260646199032 74-75 21.336010131734024 27.16779170967153 28.614825179741608 22.881372978852838 76-77 20.50938947073997 27.058088852285795 29.198592939055846 23.23392873791839 78-79 21.58045139521116 27.000331392100847 28.376490900872653 23.04272631181534 80-81 21.51468847743801 27.593783899305464 27.914954502599297 22.976573120657225 82-83 21.82615926887765 27.443098219452004 27.49353747090729 23.23720504076305 84-85 21.80213069493711 27.01560991542661 27.424102693534202 23.75815669610208 86-87 22.020912856542108 26.95761339462465 27.712301781262035 23.30917196757121 88-89 22.18197411288396 26.54747253463406 27.753270498979937 23.517282853502046 90-91 21.44483701156234 26.903804891713445 28.11987520479835 23.53148289192586 92-93 21.301609282238555 27.299671206142733 27.932063545591184 23.466655966027528 94-95 20.202358730449742 27.296027347057855 28.32461708144037 24.176996841052038 96-97 19.714324413166615 27.199925671266673 28.107963269217613 24.977786646349102 98-99 20.635606877507186 26.77737207226944 27.848501883306227 24.738519166917147 100-101 20.10648794715505 26.908264517131002 28.49530758520616 24.489939950507786 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1291.0 1 966.0 2 628.0 3 680.5 4 706.5 5 782.0 6 1080.0 7 1609.0 8 2278.0 9 2795.0 10 2965.0 11 3011.0 12 3170.5 13 3314.0 14 3576.5 15 3841.5 16 4076.0 17 4367.5 18 4697.0 19 5123.5 20 5655.5 21 6364.5 22 7395.0 23 8601.5 24 10101.5 25 12463.0 26 15421.5 27 18896.5 28 22761.5 29 26662.0 30 30676.0 31 35096.0 32 39492.5 33 43907.0 34 48865.5 35 53450.5 36 58265.5 37 63280.5 38 66582.0 39 69400.5 40 73250.0 41 77208.0 42 81201.5 43 85545.0 44 90647.0 45 96353.5 46 103699.0 47 114066.5 48 128127.0 49 126861.5 50 111886.0 51 102804.0 52 95240.0 53 88679.5 54 82474.0 55 75207.0 56 67294.5 57 59130.0 58 51225.5 59 43370.5 60 35700.5 61 28329.5 62 22150.5 63 17041.5 64 12660.0 65 9205.0 66 6551.5 67 4707.0 68 3251.0 69 2079.5 70 1332.5 71 857.0 72 558.0 73 376.5 74 226.0 75 127.0 76 73.0 77 38.5 78 16.0 79 5.5 80 3.0 81 3.0 82 2.0 83 2.0 84 2.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025489535986327427 2 0.013882349402538388 3 1.1568624502115323E-4 4 0.0 5 0.0656326630086676 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.663397759003186E-4 16-17 3.8562081673717744E-5 18-19 3.856208167371775E-4 20-21 0.0 22-23 0.0 24-25 9.640520418429437E-5 26-27 1.349672858580121E-4 28-29 9.254899601692259E-4 30-31 0.004800979168377859 32-33 0.005379410393483625 34-35 0.001812417838664734 36-37 0.08784442205272902 38-39 0.08032481612635406 40-41 0.08603200421406429 42-43 0.0896375588505569 44-45 0.09297317891533348 46-47 0.08745880123599184 48-49 0.07710488230659862 50-51 0.07120488381051981 52-53 0.06048462510522628 54-55 0.04970652327742218 56-57 0.06752220501067976 58-59 0.6990148544994815 60-61 0.3319038369656886 62-63 0.0032199338197554314 64-65 0.002545097390465371 66-67 1.0786585485772326 68-69 2.3310392750945637 70-71 2.3152673836900135 72-73 1.7787338603227416 74-75 1.2705627480264892 76-77 0.7350318387827339 78-79 0.5670361299711826 80-81 0.037983650448611975 82-83 9.640520418429437E-5 84-85 0.0011375814093746734 86-87 0.0012147055727221088 88-89 1.9281040836858872E-5 90-91 0.33419828082527486 92-93 0.056898351509570524 94-95 0.932026233012921 96-97 1.634665923189732 98-99 0.6363707528205271 100-101 0.5565086816742576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2593221.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.810160399317745 #Duplication Level Percentage of deduplicated Percentage of total 1 67.23691887363749 28.784232817363353 2 15.517892964548652 13.286469737435441 3 6.491178443590553 8.33665171052116 4 3.403574865116168 5.828303436268375 5 1.9761525453884987 4.229970372080083 6 1.220875825316765 3.1359533945676086 7 0.839561567570766 2.5159235760964993 8 0.6094412336441545 2.087222157301147 9 0.4452034370865799 1.7153307496803643 >10 2.066486110621082 15.296348612285977 >50 0.12136598787730365 3.5382035756489767 >100 0.06257622030920977 4.8208106982622 >500 0.005245068731787166 1.5050136785516652 >1k 0.002893831024429984 2.5511094884045806 >5k 4.5216109756718504E-4 1.4666479279276305 >10k+ 1.80864439026874E-4 0.9018080676049579 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12136 0.4679894231922385 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11158 0.4302757073153426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9427 0.36352474393813716 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9326 0.35962997368909166 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6710 0.2587515680306461 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6345 0.2446764082197391 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6076 0.23430320824950904 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4510 0.17391498834846703 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4334 0.1671280619738927 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4093 0.1578346002905267 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3564 0.13743525908513002 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3370 0.12995421524042877 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2966 0.11437513424424682 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2652 0.10226664059869947 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.8562081673717744E-5 0.0 12-13 0.0 0.0 0.0 3.8562081673717744E-5 0.0 14-15 0.0 0.0 0.0 3.8562081673717744E-5 0.0 16-17 0.0 0.0 0.0 3.8562081673717744E-5 0.0 18-19 0.0 0.0 0.0 5.7843122510576616E-5 0.0 20-21 0.0 0.0 0.0 2.506535308791653E-4 0.0 22-23 0.0 0.0 0.0 7.712416334743549E-4 0.0 24-25 0.0 0.0 0.0 0.0014267970219275566 0.0 26-27 0.0 0.0 0.0 0.002236600737075629 0.0 28-29 0.0 0.0 0.0 0.005398691434320484 0.0 30-31 0.0 0.0 0.0 0.01503921185274992 0.0 32-33 0.0 0.0 0.0 0.03368397834199245 0.0 34-35 0.0 0.0 0.0 0.06227776190305416 0.0 36-37 0.0 0.0 0.0 0.10380912386564817 0.0 38-39 0.0 0.0 0.0 0.16026401143597097 0.0 40-41 0.0 0.0 0.0 0.22753556291577154 0.0 42-43 0.0 0.0 0.0 0.2963688787033577 0.0 44-45 0.0 0.0 0.0 0.3766551327480381 0.0 46-47 0.0 0.0 0.0 0.4736002060757645 0.0 48-49 0.0 0.0 0.0 0.5740158667541255 0.0 50-51 0.0 0.0 0.0 0.6716936196336525 0.0 52-53 0.0 3.8562081673717744E-5 0.0 0.764261896691412 0.0 54-55 1.9281040836858872E-5 5.7843122510576616E-5 0.0 0.8700569677632566 0.0 56-57 7.712416334743549E-5 7.712416334743549E-5 0.0 0.9717451771368503 0.0 58-59 7.712416334743549E-5 7.712416334743549E-5 0.0 1.0517807776506514 0.0 60-61 7.712416334743549E-5 7.712416334743549E-5 0.0 1.1254150726066154 0.0 62-63 7.712416334743549E-5 7.712416334743549E-5 0.0 1.1902186508592982 3.8562081673717744E-5 64-65 7.712416334743549E-5 7.712416334743549E-5 0.0 1.2570081763181773 3.8562081673717744E-5 66-67 7.712416334743549E-5 7.712416334743549E-5 0.0 1.3125761360100046 3.8562081673717744E-5 68-69 9.640520418429436E-5 7.712416334743549E-5 0.0 1.3828748109011921 3.8562081673717744E-5 70-71 1.1568624502115323E-4 7.712416334743549E-5 0.0 1.4899038685865955 3.8562081673717744E-5 72-73 1.5424832669487098E-4 7.712416334743549E-5 0.0 1.6380015432545085 3.8562081673717744E-5 74-75 1.5424832669487098E-4 7.712416334743549E-5 0.0 1.8289802527435959 3.8562081673717744E-5 76-77 1.5424832669487098E-4 7.712416334743549E-5 0.0 2.068007315998135 3.8562081673717744E-5 78-79 1.5424832669487098E-4 7.712416334743549E-5 0.0 2.3684059322363966 3.8562081673717744E-5 80-81 1.5424832669487098E-4 7.712416334743549E-5 0.0 2.7295783891924366 3.8562081673717744E-5 82-83 1.5424832669487098E-4 7.712416334743549E-5 0.0 3.086316206756 3.8562081673717744E-5 84-85 1.9281040836858872E-4 7.712416334743549E-5 0.0 3.434377555942976 3.8562081673717744E-5 86-87 1.9281040836858872E-4 7.712416334743549E-5 0.0 3.7586846628189425 3.8562081673717744E-5 88-89 1.9281040836858872E-4 7.712416334743549E-5 0.0 4.055149946726484 3.8562081673717744E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12190 0.0 26.934525 1 TACATGG 12230 0.0 26.22421 2 ACATGGG 12305 0.0 24.680687 3 CATGGGA 8600 0.0 22.701513 4 GAGTACT 9245 0.0 21.61485 12-13 AGTACTT 9515 0.0 20.729078 12-13 GTACTTT 10235 0.0 19.731327 14-15 CATGGGG 4170 0.0 18.772625 4 ATGGGAG 3575 0.0 18.599276 5 ATGGGAT 3780 0.0 17.840097 5 CCTATAA 1475 0.0 16.9507 1 ACTTTTT 12160 0.0 16.898424 16-17 GCTTATA 2275 0.0 16.79606 1 TACTTTT 11825 0.0 16.779316 14-15 GTATCAA 30640 0.0 15.7965555 1 TTATAGG 1470 0.0 15.719245 3 ATAGGAG 1360 0.0 15.6036625 5 AGTACAT 9170 0.0 15.481646 2 CTATAAG 1505 0.0 15.356051 2 CTTATAG 1485 0.0 15.245258 2 >>END_MODULE