##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939603_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2101393 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52290409266615 31.0 31.0 34.0 30.0 34.0 2 31.70406106806295 31.0 31.0 34.0 30.0 34.0 3 31.75860345970506 31.0 31.0 34.0 30.0 34.0 4 35.4739541818213 37.0 35.0 37.0 33.0 37.0 5 35.242281667446306 37.0 35.0 37.0 33.0 37.0 6 35.301403878284546 37.0 35.0 37.0 32.0 37.0 7 35.260378234818525 37.0 35.0 37.0 32.0 37.0 8 35.24878164151113 37.0 35.0 37.0 32.0 37.0 9 36.81282320822426 39.0 37.0 39.0 32.0 39.0 10-11 36.69500636006687 39.0 35.0 39.0 32.0 39.0 12-13 36.654178918460275 39.0 35.0 39.0 32.0 39.0 14-15 37.54057951082925 39.0 36.0 41.0 32.0 41.0 16-17 37.54186008994986 39.0 36.0 41.0 32.0 41.0 18-19 37.65709531724909 39.0 36.5 41.0 32.0 41.0 20-21 37.6047241044393 39.0 36.0 41.0 32.0 41.0 22-23 37.477949864685 39.0 36.0 41.0 32.0 41.0 24-25 37.32016214958364 39.0 36.0 41.0 31.5 41.0 26-27 36.91201693352933 39.0 36.0 40.0 30.5 41.0 28-29 36.717426488048645 38.5 35.5 40.0 30.0 41.0 30-31 36.57886030837639 38.0 35.0 40.0 30.0 41.0 32-33 36.45501341253159 38.0 35.0 40.0 30.0 41.0 34-35 36.4679781459251 38.0 35.0 40.0 30.0 41.0 36-37 36.29871423384393 38.0 35.0 40.0 30.0 41.0 38-39 36.25111461777973 38.0 35.0 40.0 30.0 41.0 40-41 36.161509056135614 38.0 35.0 40.0 30.0 41.0 42-43 36.07007661108608 38.0 35.0 40.0 30.0 41.0 44-45 35.906941252778516 38.0 35.0 40.0 29.0 41.0 46-47 35.586044590421686 38.0 35.0 40.0 27.5 41.0 48-49 35.273378182948164 38.0 34.0 40.0 27.0 41.0 50-51 35.01884987720051 38.0 34.0 40.0 26.0 41.0 52-53 34.66409377018007 38.0 33.5 40.0 24.5 41.0 54-55 34.45188762882526 38.0 33.0 40.0 24.0 41.0 56-57 32.52944951277557 37.0 30.5 39.5 15.5 41.0 58-59 27.481399719138686 32.5 17.5 38.5 6.5 40.0 60-61 28.160257981253388 33.0 19.0 38.0 7.5 40.0 62-63 31.272033598665267 35.5 29.0 38.5 8.5 40.0 64-65 31.42067975861726 36.0 30.5 39.0 4.5 40.0 66-67 24.692407607715452 27.0 17.5 35.5 2.0 39.0 68-69 17.35749214925528 13.0 7.5 28.0 2.0 35.5 70-71 16.19325704425588 13.0 7.5 27.0 2.0 33.5 72-73 17.28418767931558 13.5 7.0 28.5 2.0 34.0 74-75 18.240714849625938 17.5 7.5 29.0 2.0 34.5 76-77 19.320225440933704 21.0 8.0 30.0 2.0 34.0 78-79 22.276606755614015 27.5 10.0 33.5 2.0 35.5 80-81 25.922846892513682 31.5 22.5 34.5 2.0 36.0 82-83 27.247067778373676 33.0 26.0 35.0 2.0 36.0 84-85 27.302043454032635 33.5 26.0 35.0 2.0 36.0 86-87 27.04648154819208 33.0 26.0 35.0 2.0 36.0 88-89 26.50521820525718 33.0 25.5 35.0 2.0 35.0 90-91 25.057624156928284 32.5 12.5 34.5 2.0 35.0 92-93 22.66376327512274 31.5 2.0 34.0 2.0 35.0 94-95 15.586390313473014 4.5 2.0 32.0 2.0 34.5 96-97 13.028632197784994 2.0 2.0 30.0 2.0 34.0 98-99 12.68692862306099 2.0 2.0 29.5 2.0 34.0 100-101 12.359061108512305 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4093786674064077 1101 2 0.3368776340197961 1101 3 0.5109276911602088 1101 4 0.33398815614848587 1101 5 0.39368091653424386 1101 6 0.43668473525568174 1101 7 0.38893918714249764 1101 8 0.4235239124613699 1101 9 0.38571686754296763 1101 10-11 0.5090676013937667 1101 12-13 0.4967438762662084 1101 14-15 0.6076167691636627 1101 16-17 0.7283849462306975 1101 18-19 0.5851390173848543 1101 20-21 0.5632702733510442 1101 22-23 0.5545465141653736 1101 24-25 0.5772705476259716 1101 26-27 0.6839921839666658 1101 28-29 0.6268689129839444 1101 30-31 0.6463914504137875 1101 32-33 0.6535612029473654 1101 34-35 0.6606024574259806 1101 36-37 0.34643478844535736 1101 38-39 0.6027409137191526 1101 40-41 0.7030696086009982 1101 42-43 0.6645015927845606 1101 44-45 0.5940404352876314 1101 46-47 0.6415349580263339 1101 48-49 0.6535252313987954 1101 50-51 0.4476951885928102 1101 52-53 0.34397946889443887 1101 54-55 0.32065066470957504 1101 56-57 -1.611365311273321 1101 58-59 -9.843569234465647 1101 60-61 -9.815673390902909 1101 62-63 -3.6116232725606707 1101 64-65 -4.581674568862908 1101 66-67 -9.552667803168564 1101 68-69 -4.8254009491705325 1101 70-71 -4.564355246295083 1101 72-73 -4.7972950899469815 1101 74-75 -6.635194260727017 1101 76-77 -7.662601088751945 1101 78-79 -11.196884102313852 1101 80-81 -8.040791793695231 1101 82-83 -4.184826756284789 1101 84-85 -3.4054100407715673 1101 86-87 -3.132137709168518 1101 88-89 -3.0733038581155903 1101 90-91 -9.442918101409312 1101 92-93 -16.50779256244296 1101 94-95 -10.96938379271982 1101 96-97 -9.107637084433751 1101 98-99 -9.084057322307341 1101 100-101 -8.924152936074368 1102 1 0.3145850810709163 1102 2 0.3676691219703976 1102 3 0.2807019788781311 1102 4 0.2165356823918856 1102 5 0.29473065260526 1102 6 0.21942239365383642 1102 7 0.3086963507349765 1102 8 0.3571060892367015 1102 9 0.4439157900821158 1102 10-11 0.37008867494052566 1102 12-13 0.2903930681705802 1102 14-15 0.464918102127335 1102 16-17 0.5083925977248498 1102 18-19 0.5036275007309641 1102 20-21 0.47522474764038947 1102 22-23 0.5839758866613067 1102 24-25 0.4839165430269219 1102 26-27 0.4778282462876078 1102 28-29 0.530752284099087 1102 30-31 0.4602185701358934 1102 32-33 0.4953587468585141 1102 34-35 0.28426106759933845 1102 36-37 0.4013658570451497 1102 38-39 0.4025840105220979 1102 40-41 0.2594402589372322 1102 42-43 0.3500673140907722 1102 44-45 0.4917139325776745 1102 46-47 0.39877771560851727 1102 48-49 0.3461081067297229 1102 50-51 0.3356799644298576 1102 52-53 0.35498443760892684 1102 54-55 0.2923435340062923 1102 56-57 -2.5107152060822635 1102 58-59 -10.219576296128807 1102 60-61 -10.3579941040276 1102 62-63 -3.8999976222516217 1102 64-65 -4.838637020820222 1102 66-67 -6.348769074702833 1102 68-69 -4.226480924272597 1102 70-71 -3.911396374095421 1102 72-73 -4.052286568655983 1102 74-75 -4.868777864512808 1102 76-77 -7.268308247180004 1102 78-79 -9.58353394384774 1102 80-81 -5.230457791705572 1102 82-83 -2.7086782167280603 1102 84-85 -2.244390598093897 1102 86-87 -2.1685927297990126 1102 88-89 -2.431239531202447 1102 90-91 -8.571645656573459 1102 92-93 -13.04685323620934 1102 94-95 -10.475489358876024 1102 96-97 -8.96307514946194 1102 98-99 -9.093118498925026 1102 100-101 -9.043195507193852 1103 1 0.183912451045817 1103 2 0.16465504170977852 1103 3 0.2310872674334199 1103 4 0.2205628092121188 1103 5 0.18665408042043197 1103 6 0.17892745056612824 1103 7 0.13165449759100056 1103 8 0.1751487724739107 1103 9 0.20078951019191038 1103 10-11 0.3726217807813299 1103 12-13 0.41192561800553307 1103 14-15 0.5637948822270218 1103 16-17 0.46526666044387355 1103 18-19 0.4559690880101712 1103 20-21 0.43896078386691073 1103 22-23 0.507521412576267 1103 24-25 0.4580835620916588 1103 26-27 0.3714214046748836 1103 28-29 0.3807697082258201 1103 30-31 0.48871215649103306 1103 32-33 0.4570442668218604 1103 34-35 0.5011283075001955 1103 36-37 0.37661331880016746 1103 38-39 0.39461271726034397 1103 40-41 0.4774912137402225 1103 42-43 0.36201556639213806 1103 44-45 0.4196012963660323 1103 46-47 0.4491268344175694 1103 48-49 0.3377242140623409 1103 50-51 0.37724017751321526 1103 52-53 0.49319447077350276 1103 54-55 0.6179764800933043 1103 56-57 -2.5548905065016214 1103 58-59 -10.13583036338985 1103 60-61 -10.805171346551866 1103 62-63 -4.40897202640522 1103 64-65 -5.405015112623836 1103 66-67 -6.198177143835501 1103 68-69 -4.218385802244976 1103 70-71 -4.13254500581203 1103 72-73 -4.315443497389348 1103 74-75 -5.05521381812156 1103 76-77 -6.48041346345453 1103 78-79 -8.98358846766006 1103 80-81 -4.984014075550466 1103 82-83 -2.7359009675473835 1103 84-85 -2.3226241929849074 1103 86-87 -2.10750525872502 1103 88-89 -2.1973465701580928 1103 90-91 -4.981689442033513 1103 92-93 -10.006255999793307 1103 94-95 -8.269290970509859 1103 96-97 -7.99282317505387 1103 98-99 -8.227596783372917 1103 100-101 -8.226282944985805 1104 1 0.24668116605004542 1104 2 0.2598917633179916 1104 3 0.21781124193876167 1104 4 0.14650665552302655 1104 5 0.22981885412853842 1104 6 0.16498751111615917 1104 7 0.22463783303521723 1104 8 0.188953720593517 1104 9 0.22763881254139307 1104 10-11 0.22545959602214083 1104 12-13 0.17709580398707914 1104 14-15 0.29985178772092524 1104 16-17 0.3362737678839025 1104 18-19 0.32156211912218424 1104 20-21 0.2781036272305428 1104 22-23 0.31042798550471673 1104 24-25 0.4480188203397617 1104 26-27 0.5210199688538992 1104 28-29 0.4487197014875264 1104 30-31 0.2959785662760339 1104 32-33 0.3288719142181975 1104 34-35 0.3990733969032192 1104 36-37 0.4892269627953567 1104 38-39 0.5073510986534444 1104 40-41 0.46043024768907514 1104 42-43 0.48086123501838784 1104 44-45 0.43798096320112023 1104 46-47 0.5061860246228278 1104 48-49 0.4567475799573586 1104 50-51 0.5386734699359934 1104 52-53 0.3302523854346404 1104 54-55 0.49915875624995465 1104 56-57 -2.7020158447123634 1104 58-59 -10.099718379630275 1104 60-61 -10.32181956365218 1104 62-63 -3.9331192485360447 1104 64-65 -5.314876511007817 1104 66-67 -6.4198812482480285 1104 68-69 -4.011618860382294 1104 70-71 -3.916708179005232 1104 72-73 -4.299633285734661 1104 74-75 -5.009816842197793 1104 76-77 -6.361350546677247 1104 78-79 -8.78565106307234 1104 80-81 -4.636204292983415 1104 82-83 -2.5027200084013046 1104 84-85 -2.0809479071530497 1104 86-87 -1.9215420809344508 1104 88-89 -1.8864698747098068 1104 90-91 -4.37867607045489 1104 92-93 -6.360824926727165 1104 94-95 -7.603970536247932 1104 96-97 -7.719200593686098 1104 98-99 -7.915567365629513 1104 100-101 -7.993130494025517 1105 1 0.34545799730164717 1105 2 0.2087010672867109 1105 3 0.26118952857472166 1105 4 0.21515812781282762 1105 5 0.24151170861419047 1105 6 0.2364500144950199 1105 7 0.25026362422315174 1105 8 0.19377868461928216 1105 9 0.384977378977986 1105 10-11 0.4820324218889809 1105 12-13 0.44287005080293085 1105 14-15 0.5796169582339132 1105 16-17 0.4969318667490512 1105 18-19 0.37404146460067267 1105 20-21 0.4248873339555175 1105 22-23 0.4664870465971376 1105 24-25 0.5655628079358976 1105 26-27 0.542752044850765 1105 28-29 0.7001740599238744 1105 30-31 0.5929476975684054 1105 32-33 0.5771227142157045 1105 34-35 0.6506818669297445 1105 36-37 0.4531985277572943 1105 38-39 0.3394388267319073 1105 40-41 0.38864703162328595 1105 42-43 0.3619857768096182 1105 44-45 0.2765582165980405 1105 46-47 0.4080064594182389 1105 48-49 0.4853065094639035 1105 50-51 0.3489541849780835 1105 52-53 0.4505413881564664 1105 54-55 0.43788479089405996 1105 56-57 1.5739913224801967 1105 58-59 -8.549509336513822 1105 60-61 -9.83133667918279 1105 62-63 -3.8442589739988478 1105 64-65 -5.140094787438066 1105 66-67 -6.094174319872671 1105 68-69 -3.581632804024288 1105 70-71 -3.774473552026649 1105 72-73 -1.8810818187082958 1105 74-75 -4.142270401305723 1105 76-77 -6.2602303717527885 1105 78-79 -8.947278102318828 1105 80-81 -4.175425358142061 1105 82-83 -1.7308062769917107 1105 84-85 -1.2246209461443947 1105 86-87 -1.2143266699538557 1105 88-89 -1.2415903716851169 1105 90-91 -3.420790352116459 1105 92-93 -5.713563496375343 1105 94-95 -7.377351673167716 1105 96-97 -7.545000412766675 1105 98-99 -8.027593110071013 1105 100-101 -7.95198046918032 1106 1 0.16221882592688175 1106 2 0.15126636655797654 1106 3 0.1069768044283208 1106 4 0.11501483787586864 1106 5 0.02471812800336437 1106 6 0.13725653214014955 1106 7 0.21435926002107664 1106 8 0.12525334277042788 1106 9 0.22308022021272933 1106 10-11 0.24187029956031125 1106 12-13 0.28225431347384955 1106 14-15 0.27186701964388504 1106 16-17 0.32110283211382296 1106 18-19 0.3491499555529387 1106 20-21 0.3281031695405474 1106 22-23 0.34507134202004863 1106 24-25 0.3485501984214494 1106 26-27 0.3656681997676614 1106 28-29 0.4941804465610602 1106 30-31 0.46700198398451676 1106 32-33 0.2200769433158385 1106 34-35 0.43218108907008457 1106 36-37 0.44245997048226116 1106 38-39 0.5092824360007242 1106 40-41 0.24362123943463843 1106 42-43 0.3103911645112518 1106 44-45 0.30770740189240087 1106 46-47 0.2664933176840236 1106 48-49 0.20265892082884562 1106 50-51 0.22782301327579546 1106 52-53 0.46939220286210315 1106 54-55 0.25900572760085083 1106 56-57 1.4529522657373803 1106 58-59 -8.662423853831438 1106 60-61 -9.708653882163222 1106 62-63 -3.318006210550063 1106 64-65 -4.8615391500025495 1106 66-67 -6.421604107803567 1106 68-69 -3.7983117526855494 1106 70-71 -4.096190165471338 1106 72-73 -4.411418345360225 1106 74-75 -5.060965644079815 1106 76-77 -6.335912022452863 1106 78-79 -8.9606183954491 1106 80-81 -4.641220003192419 1106 82-83 -2.273412516244967 1106 84-85 -1.7699251302105559 1106 86-87 -1.6847024989884893 1106 88-89 -1.7328722025211114 1106 90-91 -4.321263208908402 1106 92-93 -6.486618847230989 1106 94-95 -7.2774888221073715 1106 96-97 -7.460952676747679 1106 98-99 -6.876319876631236 1106 100-101 -7.228693807426451 1107 1 0.08317554574464836 1107 2 0.030621070375392634 1107 3 0.024588196150833852 1107 4 0.022641965845679124 1107 5 -0.0844551137736218 1107 6 -0.11571849894718866 1107 7 -0.04117428679701618 1107 8 -0.007021370290651419 1107 9 0.20018893237146074 1107 10-11 0.024187379441023893 1107 12-13 0.03265487249578314 1107 14-15 -0.004420766607260873 1107 16-17 0.06907472695607453 1107 18-19 0.20278359810902913 1107 20-21 0.14971960126394634 1107 22-23 0.16416667286639353 1107 24-25 0.21166795617126866 1107 26-27 0.15120570815849277 1107 28-29 0.17556325553622543 1107 30-31 0.20630078560876797 1107 32-33 0.2342307623820119 1107 34-35 0.15950713751131218 1107 36-37 0.100614757932874 1107 38-39 -0.17741273198097218 1107 40-41 -0.0608514862374534 1107 42-43 -0.11841277065101252 1107 44-45 -0.11642242764992972 1107 46-47 0.02490670779990012 1107 48-49 -0.15992072328987206 1107 50-51 -0.05345745507013078 1107 52-53 -0.21145139204507046 1107 54-55 -0.1462681161151096 1107 56-57 0.9261984444703373 1107 58-59 -9.290620898057995 1107 60-61 -10.800004448337994 1107 62-63 -4.38394823953557 1107 64-65 -5.730336262456191 1107 66-67 -6.944576277755036 1107 68-69 -4.170392568769536 1107 70-71 -4.09677009570364 1107 72-73 -2.6016035488860254 1107 74-75 -4.786979421549471 1107 76-77 -6.679259881552923 1107 78-79 -7.680669455720025 1107 80-81 -2.9204575104828123 1107 82-83 -1.8150816588945986 1107 84-85 -1.6221628685926923 1107 86-87 -1.563979622006329 1107 88-89 -1.3154055182474522 1107 90-91 -0.6607633904804864 1107 92-93 -3.5752529849836776 1107 94-95 -6.156347228894866 1107 96-97 -6.647092674326718 1107 98-99 -6.266318556106988 1107 100-101 -6.931107615708339 1108 1 -0.1037077712638741 1108 2 -0.0704204489981457 1108 3 -0.08667427065338273 1108 4 -0.024058685323261386 1108 5 0.03837274911449384 1108 6 -0.1530393862048598 1108 7 -0.08099106360883468 1108 8 -0.049593926239680286 1108 9 -0.06234888218214962 1108 10-11 -0.07490548412312847 1108 12-13 -0.10527734359445873 1108 14-15 -0.043278903845056504 1108 16-17 -0.1527879939578085 1108 18-19 -0.04329973789062791 1108 20-21 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2316 82-83 2.271314637862865 2316 84-85 1.9470842719938695 2316 86-87 1.8476634302808357 2316 88-89 1.9305079088318813 2316 90-91 2.8345761658461157 2316 92-93 5.011677682807466 2316 94-95 11.759844830772204 2316 96-97 13.437422075548506 2316 98-99 13.102957882284436 2316 100-101 11.834828768293804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 25.0 5 85.0 6 208.0 7 471.0 8 962.0 9 1740.0 10 2952.0 11 4975.0 12 7374.0 13 10865.0 14 13314.0 15 18071.0 16 24909.0 17 33481.0 18 37213.0 19 36663.0 20 38438.0 21 38642.0 22 38489.0 23 32998.0 24 32278.0 25 37402.0 26 52226.0 27 77499.0 28 109803.0 29 149395.0 30 184731.0 31 200909.0 32 200945.0 33 182548.0 34 163266.0 35 145592.0 36 117177.0 37 74883.0 38 28805.0 39 2048.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.49224356597479 19.1930985800854 14.276208160213166 21.038449693726644 2 13.98306234190845 23.449304813597717 39.35076276570684 23.216870078787 3 22.15102589336191 27.43315370602668 30.99246783554489 19.42335256506652 4 11.38459107839419 16.822936023866074 41.20357305844266 30.588899839297074 5 11.539253411672078 41.05478024895174 34.77794225440584 12.62802408497034 6 26.794940308642882 38.62000111354706 18.7524656263726 15.832592951437451 7 25.075140668661533 35.91904794536193 21.50098601308085 17.50482537289568 8 22.773446199706477 32.89406309425688 21.422466550297806 22.910024155738846 9 24.814587276154437 19.872722522631417 22.581639893156588 32.731050308057554 10-11 23.122186092748954 25.77892379007639 28.796802882659268 22.30208723451539 12-13 28.849077730819513 23.861528995290268 27.19891043702915 20.090482836861074 14-15 24.374429095539814 25.433804111154544 29.39001289866352 20.80175389464212 16-17 19.668262909412945 28.887909115524796 30.499911249347456 20.9439167257148 18-19 19.079276098201667 31.13498684204265 30.51418346903716 19.271553590718522 20-21 21.34919550983562 28.29168556286235 28.083680682290275 22.27543824501176 22-23 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28.12365551181196 27.157768050744053 23.506191732889533 48-49 21.903555802674894 27.376907650900424 28.37407229002916 22.345464256395523 50-51 22.422408114794422 28.29369914704611 29.306166495742374 19.977726242417095 52-53 20.93828467154154 28.90356341354731 29.604452308046252 20.553699606864896 54-55 19.1684316257166 27.535623438148164 30.81339886105966 22.48254607507557 56-57 20.811834690418348 29.10145301997768 28.48333040265173 21.603381886952246 58-59 20.4240506405586 27.40981855571948 29.951730045901765 22.214400757820155 60-61 20.902346012582154 26.77137127111514 30.285631061552877 22.040651654749826 62-63 23.497629887427685 26.42038403890216 28.962297351865285 21.11968872180487 64-65 21.81599191763843 27.86246930820388 30.10289002552061 20.218648748637076 66-67 20.30087565682748 27.068529232565595 30.43504508722262 22.195550023384307 68-69 20.87191442079348 28.580167837981342 29.20053336453684 21.347384376688336 70-71 20.86779724689797 28.20685905558666 28.534689657854322 22.390654039661044 72-73 20.136905844873418 26.99352957481284 29.096814606871913 23.77274997344183 74-75 20.608419926001343 28.47501847539466 28.784094535773097 22.132467062830905 76-77 19.881750709051836 26.977142427017604 30.17564412980353 22.965462734127033 78-79 19.733655127697126 26.91456839931319 30.841588319344886 22.5101881536448 80-81 20.658947992386864 29.33942130177937 28.719562637553608 21.282068068280154 82-83 20.61341331852408 28.58537852820206 28.242581454457028 22.55862669881683 84-85 20.824335320906638 27.51754790876515 27.9070757926896 23.75104097763861 86-87 21.63660079585569 27.910626605487998 28.49797704943309 21.954795549223228 88-89 22.658864527494785 26.21817611632684 28.55986479422306 22.563094561955317 90-91 20.4836142916159 26.625094572380192 30.10466646122318 22.786624674780732 92-93 19.105553086094798 29.071996816585095 30.001965749149868 21.82048434817024 94-95 18.754803717115394 29.10959743546122 28.74282218183345 23.39277666558994 96-97 18.275974014895567 28.314979943777256 28.147540096603286 25.26150594472389 98-99 19.146833032190695 28.322905329585318 28.004528117248512 24.525733520975475 100-101 20.68939349184187 27.846364586920576 28.145639382276844 23.318602538960718 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1288.0 1 913.5 2 550.0 3 570.0 4 576.5 5 676.0 6 904.5 7 1200.0 8 1559.0 9 1847.0 10 2009.5 11 2137.5 12 2304.0 13 2535.5 14 2784.0 15 2992.5 16 3246.5 17 3528.5 18 3787.5 19 4147.5 20 4688.0 21 5248.5 22 6046.0 23 7274.5 24 8555.5 25 10365.0 26 12907.0 27 15729.5 28 18806.5 29 22351.0 30 26427.0 31 31024.0 32 36046.0 33 41756.0 34 47561.5 35 52810.5 36 57696.0 37 61547.0 38 63566.0 39 64438.5 40 64841.0 41 65500.5 42 67735.0 43 72357.0 44 80390.0 45 92549.0 46 109715.5 47 131770.0 48 153108.5 49 140991.0 50 104752.0 51 78791.0 52 60124.5 53 47450.0 54 38437.0 55 31877.5 56 27046.5 57 22771.0 58 18902.0 59 15378.5 60 12157.0 61 9215.0 62 6701.0 63 4819.5 64 3335.5 65 2291.5 66 1576.5 67 1034.5 68 654.0 69 417.0 70 281.5 71 194.5 72 153.5 73 124.0 74 76.0 75 53.0 76 37.0 77 15.5 78 4.5 79 1.5 80 1.0 81 1.0 82 0.5 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023413040778188562 2 0.007518822038523969 3 2.379374062824041E-4 4 0.0 5 0.15013850336419698 6 0.0 7 4.7587481256480824E-5 8 0.0013800369564379439 9 0.0 10-11 0.0 12-13 0.0 14-15 5.948435157060102E-4 16-17 0.0 18-19 1.4276244376944247E-4 20-21 0.0 22-23 3.0931862816712535E-4 24-25 0.0 26-27 1.6655618439768286E-4 28-29 0.0030218050597865323 30-31 0.01211101397977437 32-33 0.002498342765965243 34-35 0.004520810719365678 36-37 0.11706520389094281 38-39 0.11582792937827432 40-41 0.12979485512705144 42-43 0.13245975407741437 44-45 0.12884310550192182 46-47 0.12115772727900016 48-49 0.12096737735397425 50-51 0.1194683716943951 52-53 0.09458011899725563 54-55 0.08194564272365996 56-57 0.5441866419084864 58-59 1.5370994383249588 60-61 0.5069970253065467 62-63 0.00578187897266242 64-65 0.11904008436308677 66-67 1.861265360644106 68-69 2.8023078024910144 70-71 2.71460407453532 72-73 2.5697477815905927 74-75 1.9615797711327678 76-77 1.3756113206810912 78-79 0.9192949629126965 80-81 0.051180336091345126 82-83 1.903499250259233E-4 84-85 1.4276244376944247E-4 86-87 3.331123687953657E-4 88-89 1.6655618439768286E-4 90-91 0.2434099666268994 92-93 0.38276990548650347 94-95 1.9577251851509925 96-97 2.3750911895109574 98-99 1.380441450028624 100-101 0.9212936371254686 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2101393.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.10797850648736 #Duplication Level Percentage of deduplicated Percentage of total 1 72.04535645931536 28.895936083624406 2 14.289942776077053 11.462814354436656 3 5.445692741308576 6.552471822640158 4 2.7149499893499796 4.355646232761483 5 1.5424973746916557 3.0933225775223057 6 0.955436364454784 2.2992372701921346 7 0.6183408603188773 1.7360281354746698 8 0.4403859101155625 1.4130390893979885 9 0.30983481243038047 1.1184163197767318 >10 1.4584578828237076 10.378145197514907 >50 0.10263934441193351 2.8452792598132755 >100 0.06382133967506567 4.655985318442042 >500 0.004652091094838529 1.306436685388264 >1k 0.004890659868932812 4.683881948690146 >5k 0.0017892658057071264 5.018684157806749 >10k+ 0.0013121282575185595 10.184675546518045 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 40174 1.9117794719978605 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 34159 1.6255407722401285 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24068 1.1453354988809803 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 22360 1.064056080894911 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 15861 0.7547850402090422 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 15453 0.7353693478563982 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 14510 0.6904943530315367 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 13539 0.6442869087314939 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 12270 0.5838983950170197 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10246 0.4875813329539025 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 10239 0.48724822058510714 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9988 0.4753037627897304 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8992 0.42790663145827557 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 8827 0.4200546970509562 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7947 0.3781777135452531 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 7269 0.34591340125335907 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 7171 0.34124982809022397 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 6715 0.3195499366372687 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6644 0.3161712254680586 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 6429 0.3059399169979152 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6425 0.30574956707288925 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5940 0.2826696386634961 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5876 0.2796240398630813 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5696 0.27105829323691477 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 5512 0.2623021966857223 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5469 0.2602559349916936 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4894 0.23289313326921712 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4709 0.2240894492367682 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4650 0.22128178784263583 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4463 0.21238292884767387 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3742 0.17807235486175121 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3719 0.17697784279285217 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3673 0.17478881865505405 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3636 0.17302808184856425 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3260 0.15513518889612746 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3159 0.1503288532892229 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3150 0.14990056595791457 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3091 0.1470929045637822 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3042 0.14476111798221464 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2775 0.13205526048673427 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2617 0.12453643844821032 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2589 0.12320398897302884 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2521 0.11996804024758814 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2289 0.10892774459608459 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2241 0.10664354549577353 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2196 0.10450210883923187 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2195 0.1044545213579754 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 9.517496251296163E-5 0.0 9 0.0 0.0 0.0 9.517496251296163E-5 0.0 10-11 0.0 0.0 0.0 1.1896870314120206E-4 0.0 12-13 0.0 0.0 0.0 2.3793740628240412E-4 0.0 14-15 0.0 0.0 0.0 3.0931862816712535E-4 0.0 16-17 0.0 0.0 0.0 4.282873313083274E-4 0.0 18-19 0.0 0.0 0.0 5.948435157060103E-4 0.0 20-21 0.0 0.0 0.0 0.0010231308470143377 0.0 22-23 0.0 0.0 0.0 0.002141436656541637 0.0 24-25 0.0 0.0 0.0 0.0038545859817749467 0.0 26-27 0.0 0.0 0.0 0.005329797900725852 0.0 28-29 0.0 0.0 0.0 0.01035027717328458 0.0 30-31 0.0 0.0 0.0 0.0231988971125344 0.0 32-33 0.0 0.0 0.0 0.05148965471951225 0.0 34-35 0.0 0.0 0.0 0.10383588410164116 0.0 36-37 0.0 0.0 0.0 0.17336119421735963 0.0 38-39 0.0 0.0 0.0 0.2579955296320108 0.0 40-41 0.0 0.0 0.0 0.351742867707278 0.0 42-43 0.0 0.0 0.0 0.4501537789456803 0.0 44-45 0.0 0.0 0.0 0.5606043229419723 0.0 46-47 0.0 0.0 0.0 0.6938968579413751 0.0 48-49 0.0 0.0 0.0 0.8205509393054988 0.0 50-51 0.0 0.0 0.0 0.9320959953706898 0.0 52-53 0.0 0.0 0.0 1.031767974862389 0.0 54-55 0.0 0.0 0.0 1.1381022017299953 0.0 56-57 0.0 0.0 0.0 1.2306836465144788 0.0 58-59 0.0 0.0 0.0 1.3034449053556378 0.0 60-61 2.379374062824041E-5 0.0 0.0 1.3736840276902036 0.0 62-63 9.517496251296163E-5 0.0 0.0 1.4269106254755775 0.0 64-65 9.517496251296163E-5 4.758748125648082E-5 0.0 1.4694062462376147 0.0 66-67 9.517496251296163E-5 4.758748125648082E-5 0.0 1.5085231558304422 0.0 68-69 9.517496251296163E-5 4.758748125648082E-5 0.0 1.5599652230686978 0.0 70-71 9.517496251296163E-5 4.758748125648082E-5 0.0 1.6418870720517296 0.0 72-73 1.6655618439768288E-4 4.758748125648082E-5 0.0 1.7664473042405682 0.0 74-75 1.9034992502592327E-4 4.758748125648082E-5 0.0 1.9214873181741825 0.0 76-77 1.9034992502592327E-4 4.758748125648082E-5 0.0 2.1346792342032166 0.0 78-79 1.9034992502592327E-4 4.758748125648082E-5 0.0 2.4828530408162583 0.0 80-81 1.9034992502592327E-4 4.758748125648082E-5 0.0 2.9062864490364246 0.0 82-83 1.9034992502592327E-4 4.758748125648082E-5 0.0 3.3057119729626967 0.0 84-85 1.9034992502592327E-4 7.138122188472122E-5 0.0 3.689005340743021 0.0 86-87 1.9034992502592327E-4 1.4276244376944247E-4 0.0 4.00044161182606 0.0 88-89 1.9034992502592327E-4 1.4276244376944247E-4 0.0 4.239711467583646 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGAT 65 2.5461736E-4 22.397902 70-71 GCTTACG 715 0.0 21.819567 60-61 GTACATG 11585 0.0 21.427164 1 CATGGGG 1850 0.0 21.381779 4 TACATGG 11410 0.0 21.379673 2 GTACGCT 980 0.0 21.307734 54-55 GCTTATA 3745 0.0 21.25617 1 CGTGTAC 880 0.0 21.238222 76-77 TGGTAGC 2335 0.0 21.197048 62-63 ACATGGG 11165 0.0 20.79335 3 AGTACTT 7995 0.0 20.673912 12-13 GGTATCG 505 0.0 20.658995 62-63 GAGTACT 8080 0.0 19.699858 12-13 ACTGCGG 595 0.0 19.572952 62-63 GTATCGA 435 0.0 19.521399 62-63 GTAGCAA 1830 0.0 19.51551 64-65 GTACTTT 8385 0.0 19.011328 14-15 ATACTCT 3735 0.0 18.911976 5 CGGCTTG 730 0.0 18.909023 74-75 ATGGGAG 2715 0.0 18.905706 5 >>END_MODULE