##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939602_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2035979 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.337489237364432 2.0 2.0 31.0 2.0 33.0 2 22.59363873595946 16.0 16.0 31.0 16.0 34.0 3 27.0678219176131 28.0 25.0 31.0 16.0 34.0 4 32.21636274244479 35.0 32.0 35.0 25.0 37.0 5 32.829405411352475 35.0 33.0 35.0 26.0 37.0 6 33.283318246406274 35.0 33.0 37.0 28.0 37.0 7 33.48985033735613 35.0 33.0 37.0 28.0 37.0 8 33.44559300464297 35.0 33.0 37.0 27.0 37.0 9 34.738653984152094 37.0 34.0 39.0 27.0 39.0 10-11 34.58277221916336 37.0 33.5 39.0 27.0 39.0 12-13 34.60555634414697 37.0 34.0 39.0 27.0 39.0 14-15 35.123903782897564 38.0 33.0 40.0 26.5 41.0 16-17 34.82456842629516 38.0 33.0 40.0 25.0 41.0 18-19 35.28819648925652 38.0 34.0 40.0 27.0 41.0 20-21 35.310758608021004 38.0 34.0 40.0 26.5 41.0 22-23 35.17800699319591 38.0 34.0 40.0 26.0 41.0 24-25 34.969646297923504 38.0 34.0 40.0 25.0 41.0 26-27 34.9481563906111 38.0 33.5 40.0 25.0 41.0 28-29 34.72186329033846 38.0 33.0 40.0 24.0 41.0 30-31 34.65901833958013 38.0 33.0 40.0 24.0 41.0 32-33 34.40850986184042 38.0 33.0 40.0 24.0 41.0 34-35 34.16809775542872 38.0 33.0 40.0 24.0 41.0 36-37 33.79869193149831 38.0 32.5 40.0 21.5 41.0 38-39 33.535051687664755 37.5 31.5 40.0 19.5 41.0 40-41 33.18468019562088 37.0 31.0 40.0 17.0 41.0 42-43 33.00433722548219 37.0 31.0 40.0 16.5 41.0 44-45 32.71874808138983 37.0 31.0 40.0 15.0 41.0 46-47 32.35054929348486 37.0 30.0 40.0 14.0 41.0 48-49 31.96703109413211 37.0 30.0 39.0 9.0 40.0 50-51 31.27554434500552 35.5 29.5 38.5 9.0 40.0 52-53 31.245281508306324 36.0 29.5 39.0 8.0 40.0 54-55 31.425058902866876 36.0 30.0 39.0 8.0 40.0 56-57 31.222614771566896 36.0 29.0 39.0 6.5 40.0 58-59 30.872943188510295 36.0 28.0 39.0 2.0 40.0 60-61 30.208797340247614 35.0 28.0 38.5 2.0 40.0 62-63 29.750743254228063 34.5 27.5 38.0 2.0 40.0 64-65 29.0551999799605 34.0 26.0 38.0 2.0 40.0 66-67 28.43841807798607 33.5 26.0 37.5 2.0 39.0 68-69 28.164806218531723 33.5 26.0 37.0 2.0 39.0 70-71 27.54537080195817 33.0 24.5 36.0 2.0 39.0 72-73 26.893603519486202 32.0 23.0 36.0 2.0 38.0 74-75 26.302565006809992 32.0 22.0 35.0 2.0 37.5 76-77 25.585854274528373 31.0 20.5 35.0 2.0 37.0 78-79 24.890428142922886 31.0 18.0 34.0 2.0 36.0 80-81 24.15461849066223 30.5 12.0 34.0 2.0 36.0 82-83 23.474203073803807 30.0 7.5 34.0 2.0 35.0 84-85 22.790457318076463 29.0 3.5 34.0 2.0 35.0 86-87 22.02201569859021 29.0 2.0 34.0 2.0 35.0 88-89 21.0520292694571 28.0 2.0 33.0 2.0 35.0 90-91 19.984818114528686 26.0 2.0 32.5 2.0 34.0 92-93 19.005872604776375 25.0 2.0 32.0 2.0 34.0 94-95 18.076876775251613 24.5 2.0 32.0 2.0 34.0 96-97 17.080406035622175 20.0 2.0 31.0 2.0 34.0 98-99 16.023407657937533 10.0 2.0 31.0 2.0 34.0 100-101 14.452686398042417 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.818963784662735 1101 2 7.923385107863691 1101 3 3.768729533610877 1101 4 2.3930362117610784 1101 5 1.6086441208489077 1101 6 1.2638609546204052 1101 7 0.933742904864971 1101 8 1.0947613333855415 1101 9 0.9203985951647908 1101 10-11 1.006294299989321 1101 12-13 0.6862766881211826 1101 14-15 0.7551689360350977 1101 16-17 0.7235640592487655 1101 18-19 0.7568106666427141 1101 20-21 0.7220047383060404 1101 22-23 0.6522651878019659 1101 24-25 0.8454625896576502 1101 26-27 0.8991176196491111 1101 28-29 0.9355819429309449 1101 30-31 0.9321922857620066 1101 32-33 0.9820976071971046 1101 34-35 1.1737317974659334 1101 36-37 1.2766049015919876 1101 38-39 1.0707301293153506 1101 40-41 1.2239642449404826 1101 42-43 1.1308870537165419 1101 44-45 1.0522831499944942 1101 46-47 0.6402991545867636 1101 48-49 0.560405129909455 1101 50-51 0.650297679814198 1101 52-53 0.7779079843582544 1101 54-55 0.7651668927671977 1101 56-57 0.5310307017491631 1101 58-59 0.5743716148488609 1101 60-61 0.5342864568235761 1101 62-63 0.5027944919275349 1101 64-65 0.35828088754600174 1101 66-67 0.45607862503931074 1101 68-69 0.2408690572132599 1101 70-71 0.22909141000191013 1101 72-73 0.2311006815872254 1101 74-75 0.28096898946674287 1101 76-77 -0.07245474002735364 1101 78-79 -0.2781491286084581 1101 80-81 -0.2529626128620208 1101 82-83 -0.2106427765036223 1101 84-85 -0.3932511517247512 1101 86-87 -0.5550096850485566 1101 88-89 -0.33258322566519993 1101 90-91 -0.20099097407399924 1101 92-93 0.04798977752476574 1101 94-95 -0.15309187376258393 1101 96-97 -0.7393635085637058 1101 98-99 -0.7796610455050708 1101 100-101 -0.9813517142119625 1102 1 14.832611524087639 1102 2 7.95961631924677 1102 3 3.8168595835158214 1102 4 2.1730738632582245 1102 5 1.593800742165449 1102 6 1.2449535164074774 1102 7 1.0229646996605979 1102 8 0.8476371799872524 1102 9 1.0082855069993926 1102 10-11 1.086367783532566 1102 12-13 0.772444404375932 1102 14-15 0.7533262777057246 1102 16-17 1.062825770572438 1102 18-19 1.03888406107 1102 20-21 1.0054520039310404 1102 22-23 1.12663692021642 1102 24-25 1.0614305004822668 1102 26-27 1.0985081035488307 1102 28-29 1.1483258978976707 1102 30-31 1.1177994996997498 1102 32-33 1.1764502539413968 1102 34-35 1.054462139968308 1102 36-37 1.1779637344698415 1102 38-39 1.012841880741206 1102 40-41 1.0556668798026507 1102 42-43 1.023898690776896 1102 44-45 0.9500170051513379 1102 46-47 1.082363563153777 1102 48-49 1.062971855491682 1102 50-51 0.8801240522001272 1102 52-53 0.9848315525616762 1102 54-55 0.8802146812058851 1102 56-57 0.8524441552900335 1102 58-59 0.7242396118189198 1102 60-61 1.0148610320374551 1102 62-63 0.7754652232725938 1102 64-65 0.7783076594685312 1102 66-67 0.8178978161633594 1102 68-69 0.3695116724699119 1102 70-71 0.6454087296905975 1102 72-73 0.678228919467454 1102 74-75 0.6614217728555261 1102 76-77 0.7179574955952717 1102 78-79 0.5668735588321319 1102 80-81 0.7034401838941591 1102 82-83 0.5652649067782214 1102 84-85 0.47968989806136975 1102 86-87 0.7044440340579072 1102 88-89 0.6872283683224438 1102 90-91 0.5766916022686814 1102 92-93 0.708795831469633 1102 94-95 0.7596812295525304 1102 96-97 -0.16898045842111742 1102 98-99 -0.20781036673843722 1102 100-101 -0.36437772122241974 1103 1 14.761842757654549 1103 2 7.860402851916255 1103 3 3.80058484477604 1103 4 2.3100369737301136 1103 5 1.4468992766539657 1103 6 0.9422451353275747 1103 7 0.6424827916263638 1103 8 0.7849775861293722 1103 9 0.8609617662907993 1103 10-11 0.9472267526397147 1103 12-13 0.6892643112227503 1103 14-15 0.6796194052671254 1103 16-17 0.9316679289761609 1103 18-19 0.8231199324456924 1103 20-21 0.7899204424692527 1103 22-23 0.7841479372680311 1103 24-25 1.058463408171825 1103 26-27 1.064654580708968 1103 28-29 1.0736691318399423 1103 30-31 0.8802399255119511 1103 32-33 0.8784436319805664 1103 34-35 0.8585353586978357 1103 36-37 1.0134733340121969 1103 38-39 1.0088910586580155 1103 40-41 0.9944676543347626 1103 42-43 0.9208041038396502 1103 44-45 1.0106624455717395 1103 46-47 0.8125320807332415 1103 48-49 0.6933558642547979 1103 50-51 0.8344731032539237 1103 52-53 0.9587648565402667 1103 54-55 0.7601078401672368 1103 56-57 0.7570882350459165 1103 58-59 0.661373391849633 1103 60-61 0.7401360522940301 1103 62-63 0.5180985286948605 1103 64-65 0.6963584127966556 1103 66-67 0.5965354959235647 1103 68-69 0.5151264618466271 1103 70-71 0.5560921117179163 1103 72-73 0.5404327628935839 1103 74-75 0.23521492748584905 1103 76-77 0.5145605939041857 1103 78-79 0.26288955476096376 1103 80-81 0.17585898163697067 1103 82-83 -0.1263143799015225 1103 84-85 -0.06579750947570062 1103 86-87 0.02713187433682407 1103 88-89 -0.10128506959317818 1103 90-91 -0.19594907041209808 1103 92-93 0.04384390587668818 1103 94-95 0.1470598479907963 1103 96-97 -0.461814434791755 1103 98-99 -0.8860135451713607 1103 100-101 -0.7136370929346736 1104 1 14.758514565912733 1104 2 7.8921170233729505 1104 3 3.698142149351618 1104 4 2.3029428957888527 1104 5 1.4644492423766877 1104 6 1.2219338712870709 1104 7 0.9649675865779344 1104 8 0.8887898345429477 1104 9 0.947633838220348 1104 10-11 0.8558078271883929 1104 12-13 0.6636349057137778 1104 14-15 0.6694032386971358 1104 16-17 1.055329467234877 1104 18-19 0.900081427638078 1104 20-21 0.959387189116228 1104 22-23 0.9602078961352944 1104 24-25 0.9782371122270987 1104 26-27 0.9164950068018882 1104 28-29 1.004436832977241 1104 30-31 1.02364374698886 1104 32-33 1.0931327634471018 1104 34-35 1.0559601684150124 1104 36-37 1.1605947019392104 1104 38-39 1.1091126524634944 1104 40-41 1.2132256743386307 1104 42-43 0.9750041688785771 1104 44-45 0.731062808559308 1104 46-47 0.7716450023876931 1104 48-49 0.5781369730807526 1104 50-51 0.9626168349067932 1104 52-53 1.102785009821222 1104 54-55 0.7587414732070101 1104 56-57 0.9775620964250926 1104 58-59 0.9767533422794159 1104 60-61 0.8188965552032066 1104 62-63 0.7548308055849411 1104 64-65 0.7846571172913741 1104 66-67 0.8729930492291231 1104 68-69 0.8843080039725209 1104 70-71 0.5271762624324658 1104 72-73 0.3649042116798178 1104 74-75 0.4015455230315581 1104 76-77 0.3921539115930166 1104 78-79 0.20071914396098478 1104 80-81 -0.09715568288516607 1104 82-83 0.012745895371377713 1104 84-85 -0.14795673621549454 1104 86-87 -0.37386095819670473 1104 88-89 -0.30787619441520064 1104 90-91 0.08766100798618837 1104 92-93 0.1834860159506917 1104 94-95 -0.027883902119064885 1104 96-97 -0.3598857886562996 1104 98-99 -0.5676945379819234 1104 100-101 -0.7087931204619622 1105 1 14.719416821551832 1105 2 7.855602125015945 1105 3 3.846311183046968 1105 4 2.333153839815587 1105 5 1.5617654495613138 1105 6 1.1000040467256653 1105 7 0.9334895860209826 1105 8 1.050085434654342 1105 9 1.0819279067249425 1105 10-11 0.9727111967233455 1105 12-13 0.7533731401888559 1105 14-15 0.82104101369017 1105 16-17 1.2259852734170025 1105 18-19 1.1618069219293687 1105 20-21 1.0970873422769074 1105 22-23 0.8134347131779052 1105 24-25 1.0637738707901647 1105 26-27 0.9036608380466689 1105 28-29 1.141082266004254 1105 30-31 1.23537272220468 1105 32-33 0.8065256205899658 1105 34-35 1.0034556432131154 1105 36-37 0.9760604496417997 1105 38-39 0.9547614967965501 1105 40-41 1.2943538419799623 1105 42-43 1.1412566055257543 1105 44-45 0.8789359636693064 1105 46-47 0.6548356178151451 1105 48-49 0.754820000092721 1105 50-51 0.7664058212615679 1105 52-53 0.8605927235499884 1105 54-55 0.9598571095200157 1105 56-57 1.07503842613826 1105 58-59 0.9894308694306275 1105 60-61 0.7781297075284641 1105 62-63 0.691267383139941 1105 64-65 0.9884408822593471 1105 66-67 0.9859977832864928 1105 68-69 0.8551709251643871 1105 70-71 0.776272962516007 1105 72-73 0.7507420702150434 1105 74-75 0.6977739412994488 1105 76-77 0.7297252705459556 1105 78-79 0.7943386092909783 1105 80-81 0.5766033675186186 1105 82-83 0.6807440156721292 1105 84-85 0.3491749819688579 1105 86-87 0.2581217764176067 1105 88-89 0.34809185069758186 1105 90-91 0.7284156724250614 1105 92-93 0.7741484358894262 1105 94-95 0.6079678801142947 1105 96-97 0.41466169115433615 1105 98-99 0.2030012988323353 1105 100-101 0.34022003743277374 1106 1 14.821501127543875 1106 2 7.918944418423237 1106 3 3.5044241148203312 1106 4 2.221944543404959 1106 5 1.5445027899001147 1106 6 1.1214511712561759 1106 7 0.8495834002600162 1106 8 0.7580042992393388 1106 9 0.9867840287259568 1106 10-11 0.8286927429196993 1106 12-13 0.639827067523413 1106 14-15 0.8652786205664924 1106 16-17 1.017046205984748 1106 18-19 0.9816225493900461 1106 20-21 0.8869394923462437 1106 22-23 0.7467772504757377 1106 24-25 0.8341228346037823 1106 26-27 0.6152232531204973 1106 28-29 0.9680976729182049 1106 30-31 0.7453785494465492 1106 32-33 0.7375257198882537 1106 34-35 0.9181233510601174 1106 36-37 0.8512895325436816 1106 38-39 1.021489295788939 1106 40-41 1.1524964325852878 1106 42-43 0.850374453435613 1106 44-45 0.5216291336888901 1106 46-47 0.7486616158500894 1106 48-49 0.8474685729846385 1106 50-51 0.4893187279490441 1106 52-53 0.4062377095088543 1106 54-55 0.5328226268815399 1106 56-57 0.6650166153555119 1106 58-59 0.6821801778267336 1106 60-61 0.4596156280758912 1106 62-63 0.37792617852971233 1106 64-65 0.34433772004426544 1106 66-67 0.3388026551742733 1106 68-69 0.45412080217387185 1106 70-71 0.3840917448750574 1106 72-73 0.4847363178824047 1106 74-75 0.40965123648365775 1106 76-77 0.5833277951614804 1106 78-79 0.6213696948826204 1106 80-81 0.48099130678290436 1106 82-83 0.5129775913862069 1106 84-85 0.3714158395576881 1106 86-87 0.42119984055385373 1106 88-89 0.26129665081186104 1106 90-91 0.6790223936999809 1106 92-93 1.201860368059986 1106 94-95 0.6865679222725163 1106 96-97 0.19430898187883372 1106 98-99 0.10334174389773132 1106 100-101 0.12588598055750033 1107 1 14.672826160115633 1107 2 7.932676604693402 1107 3 3.6483434375963846 1107 4 2.2640238136632505 1107 5 1.3525229138742674 1107 6 1.1640534365044601 1107 7 0.8259176670932291 1107 8 0.8229585140920648 1107 9 0.8187087174474001 1107 10-11 0.7914257820998287 1107 12-13 0.487708979787854 1107 14-15 0.6781089552832853 1107 16-17 0.9021440807614454 1107 18-19 0.6787599703111908 1107 20-21 0.7735679459600249 1107 22-23 0.7819923164251534 1107 24-25 0.7793995518406192 1107 26-27 0.8236357072849856 1107 28-29 1.0042456091447107 1107 30-31 0.8521813878906315 1107 32-33 0.8986581257659481 1107 34-35 0.6453371812974495 1107 36-37 0.7339793637749565 1107 38-39 0.7351039970689754 1107 40-41 0.9626771638716392 1107 42-43 0.9140087985082062 1107 44-45 0.8913838633097129 1107 46-47 0.8207190764617636 1107 48-49 0.6615961115670643 1107 50-51 0.8652688075315886 1107 52-53 0.6458455975828912 1107 54-55 0.8123143395516195 1107 56-57 0.7621434168759755 1107 58-59 0.9758626417963256 1107 60-61 0.845335766153287 1107 62-63 0.6557114416557965 1107 64-65 0.5665965295296971 1107 66-67 0.3511583068780766 1107 68-69 0.35475888964079516 1107 70-71 0.44933809818126136 1107 72-73 0.32670473502925645 1107 74-75 0.2956528096660165 1107 76-77 0.3229610774545293 1107 78-79 0.4236207140092958 1107 80-81 0.42531311743689315 1107 82-83 0.4255629243568855 1107 84-85 0.5176481349600301 1107 86-87 0.4496042189369511 1107 88-89 0.4423049650050892 1107 90-91 0.5268417648299852 1107 92-93 0.6100510320537502 1107 94-95 0.6478910576232835 1107 96-97 0.21717238364643876 1107 98-99 0.10653050227572614 1107 100-101 0.05311811142209599 1108 1 14.362996974573086 1108 2 7.365294615714955 1108 3 3.3283813364412786 1108 4 -0.2614947827557259 1108 5 0.008638086159578506 1108 6 0.2963531315486847 1108 7 0.4157901445892591 1108 8 0.4333107085969772 1108 9 0.5942941148704719 1108 10-11 0.3742602528887886 1108 12-13 -0.020508769422789896 1108 14-15 0.2249180735590599 1108 16-17 0.5135096281129421 1108 18-19 0.08399048893212324 1108 20-21 0.05399733546013863 1108 22-23 -0.6906152883482335 1108 24-25 -0.029674292073011088 1108 26-27 0.13373301612379151 1108 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2312 4 -1.6458707996411341 2312 5 -1.8314187675517708 2312 6 -0.6108464616082401 2312 7 -0.21398858707271984 2312 8 -0.03325839634234029 2312 9 -0.020126053734685456 2312 10-11 -0.022035957792382987 2312 12-13 -0.7499853916532402 2312 14-15 -0.19546450355234413 2312 16-17 -0.36951083791915806 2312 18-19 -0.2751892931484079 2312 20-21 -0.14747178056305188 2312 22-23 0.15266299907003855 2312 24-25 0.02441082371782244 2312 26-27 0.030548153246620302 2312 28-29 -0.5109708299419182 2312 30-31 -0.08935571765318429 2312 32-33 0.03327731009625268 2312 34-35 -0.9441323025053237 2312 36-37 -0.29152787069913444 2312 38-39 0.10532773252384686 2312 40-41 0.03946429798198636 2312 42-43 0.07048465264750092 2312 44-45 0.2078078929998597 2312 46-47 0.16029419691729885 2312 48-49 0.2253634538322018 2312 50-51 0.050091587912096 2312 52-53 -0.05263853132834484 2312 54-55 -0.09856403367963651 2312 56-57 -0.08503159121787363 2312 58-59 -0.07707203368788029 2312 60-61 0.1651238024899122 2312 62-63 0.06578191766942965 2312 64-65 0.13435902024557578 2312 66-67 0.01639932508448183 2312 68-69 0.3698116663076547 2312 70-71 0.367845520718344 2312 72-73 0.18520309959533066 2312 74-75 0.4855288904331232 2312 76-77 0.45029044003999275 2312 78-79 0.4141535869453641 2312 80-81 0.5078885449826451 2312 82-83 0.6542264034747518 2312 84-85 0.56105765761329 2312 86-87 0.4991858977920067 2312 88-89 0.997462176149849 2312 90-91 1.1748449642815082 2312 92-93 1.6851991614220516 2312 94-95 1.57936411462304 2312 96-97 1.270833720274446 2312 98-99 0.9212795214914795 2312 100-101 0.965492359170101 2313 1 -14.203985434087265 2313 2 -7.4078889891330615 2313 3 -3.619492530783063 2313 4 -1.0708305529845923 2313 5 -0.5241510462236008 2313 6 -0.801821539968337 2313 7 -0.27618801707767204 2313 8 -0.06504614429302791 2313 9 -2.073343795257287 2313 10-11 -0.47056603043546374 2313 12-13 -0.586452275623401 2313 14-15 -0.9063242745303768 2313 16-17 -0.7915770984216906 2313 18-19 -0.21893612887947 2313 20-21 -0.9295047060256678 2313 22-23 -2.0193375584101574 2313 24-25 -1.177868587628602 2313 26-27 -0.34115290084601924 2313 28-29 0.031057203347607754 2313 30-31 -1.4567795334344211 2313 32-33 -0.5235339032195583 2313 34-35 -1.4116203991655567 2313 36-37 -1.6123309834864799 2313 38-39 -1.0106534610526126 2313 40-41 -0.3125198764069168 2313 42-43 -0.24498741361300347 2313 44-45 -0.41229637854988255 2313 46-47 -0.9227874170447308 2313 48-49 -0.30057274312896354 2313 50-51 -1.1476435066035506 2313 52-53 -0.32830565876944817 2313 54-55 -1.0849965017809637 2313 56-57 -0.6627036563406641 2313 58-59 -0.45066368513760935 2313 60-61 -1.8559441133874621 2313 62-63 -0.6518731386189991 2313 64-65 -0.3708864132521583 2313 66-67 -1.6509397309536524 2313 68-69 -0.4677272293127146 2313 70-71 -0.006490764984562247 2313 72-73 -1.1332890302634233 2313 74-75 -0.5349954822594469 2313 76-77 0.007957783646887151 2313 78-79 0.09063617137801216 2313 80-81 -0.008450175429661755 2313 82-83 -0.01993808731260671 2313 84-85 0.11635002184126719 2313 86-87 0.021121605535856958 2313 88-89 0.19423419519518959 2313 90-91 0.8801543942295709 2313 92-93 1.2813696138342898 2313 94-95 1.327298878063715 2313 96-97 0.7862511762306674 2313 98-99 0.7958456255582416 2313 100-101 0.8404222475034064 2314 1 -14.203985434087265 2314 2 -7.2820162884944235 2314 3 -3.606001400127422 2314 4 -1.5138339903259705 2314 5 -1.4456513173895011 2314 6 -6.179346036574422 2314 7 -1.9264895805758435 2314 8 -4.4851467748261165 2314 9 -1.7927467284809993 2314 10-11 -0.6323350163908117 2314 12-13 -3.1662096931204573 2314 14-15 -3.118609054693003 2314 16-17 -2.967058393764777 2314 18-19 -1.7072873823221215 2314 20-21 -2.2819421110934783 2314 22-23 -1.2717545054102501 2314 24-25 -3.2712312956591703 2314 26-27 -5.003034888675401 2314 28-29 -2.181730729480442 2314 30-31 -2.2355643242120777 2314 32-33 -4.229270268206932 2314 34-35 -3.0003252297785465 2314 36-37 -1.1061493660253205 2314 38-39 -1.7488744390640534 2314 40-41 -2.666074948174206 2314 42-43 -1.6681240406952398 2314 44-45 -2.673417075359385 2314 46-47 -2.39316857400636 2314 48-49 -1.4410471965994063 2314 50-51 -2.37904229803355 2314 52-53 -0.9680190142206584 2314 54-55 -0.4514171891334371 2314 56-57 -1.1810934327108242 2314 58-59 -1.7752132698967351 2314 60-61 -0.8033183396961512 2314 62-63 -2.9697801238911232 2314 64-65 -1.141252709164597 2314 66-67 -2.483565523945032 2314 68-69 -2.202617884800784 2314 70-71 -2.7912721821110544 2314 72-73 -2.9758465396287797 2314 74-75 -2.1958344946365784 2314 76-77 -0.9953670383582711 2314 78-79 -0.460204346404101 2314 80-81 -0.10913069568218248 2314 82-83 -0.28796743498974564 2314 84-85 -0.07047112253671983 2314 86-87 0.21463215205576702 2314 88-89 0.4130723120493549 2314 90-91 0.7600633172281057 2314 92-93 1.572450861313218 2314 94-95 1.5539653903298714 2314 96-97 1.0589634914084307 2314 98-99 1.1078532568034998 2314 100-101 1.0946592870280067 2315 1 -14.203985434087265 2315 2 -7.345583779839901 2315 3 -4.510799171487292 2315 4 -2.719736711529304 2315 5 -3.2783441534287583 2315 6 -2.6562064678472446 2315 7 -3.0517827480940873 2315 8 -3.4418549668849856 2315 9 -2.254145501360199 2315 10-11 -4.523673988965108 2315 12-13 -4.219327499462487 2315 14-15 -3.0005264801783724 2315 16-17 -4.0929664943893975 2315 18-19 -1.2598304420629418 2315 20-21 -3.221176733239865 2315 22-23 -4.175836280571527 2315 24-25 -4.933434574519893 2315 26-27 -4.452157380525197 2315 28-29 -2.4359887582766646 2315 30-31 -3.1012558514047157 2315 32-33 -4.226389771135672 2315 34-35 -3.7747850346193523 2315 36-37 -4.777730822380292 2315 38-39 -3.276677731293752 2315 40-41 -4.491961741993364 2315 42-43 -3.251451654414595 2315 44-45 -1.5782661740377435 2315 46-47 -1.1124580765949084 2315 48-49 -0.7743005548085833 2315 50-51 -0.741407596150772 2315 52-53 -0.32669401739877557 2315 54-55 -1.9103431988144095 2315 56-57 -4.071673735792672 2315 58-59 -3.584759375257395 2315 60-61 -4.387018772775374 2315 62-63 -2.36237691418302 2315 64-65 -3.086839982217775 2315 66-67 -1.858396397446466 2315 68-69 -1.325115802363939 2315 70-71 -1.9710639205215692 2315 72-73 -1.9642819775214342 2315 74-75 -2.424394347561929 2315 76-77 -2.0180005953235387 2315 78-79 -1.741131592317462 2315 80-81 -1.3152363388423325 2315 82-83 -0.8705651264938616 2315 84-85 -0.7521038803475761 2315 86-87 -1.6551053803296831 2315 88-89 -2.3101686977619202 2315 90-91 -1.6190073320405851 2315 92-93 -0.6826005525455159 2315 94-95 -0.4395862671346862 2315 96-97 -1.2773640349749051 2315 98-99 -0.6251019453893303 2315 100-101 -0.2548077344735642 2316 1 -14.203985434087265 2316 2 -7.32178701401271 2316 3 -5.146077972832423 2316 4 -2.7839227349696465 2316 5 -2.1047225291258798 2316 6 -0.9766859553975209 2316 7 -4.602729800410131 2316 8 -2.9154015262485906 2316 9 -1.3350900275100201 2316 10-11 -4.3640444853437685 2316 12-13 -3.484103921751071 2316 14-15 -1.6577223236648706 2316 16-17 -3.061168802101246 2316 18-19 -2.278329059271954 2316 20-21 -3.202806624486712 2316 22-23 -2.7931725012948547 2316 24-25 -3.150875082105294 2316 26-27 -3.8833132505814483 2316 28-29 -3.26992143104653 2316 30-31 -2.4666661954053453 2316 32-33 -2.5409102665842696 2316 34-35 -3.974725290688202 2316 36-37 -2.093860823734378 2316 38-39 -3.1354020353529606 2316 40-41 -2.4342837467812792 2316 42-43 -6.432320855608033 2316 44-45 -3.1181059954979986 2316 46-47 -4.301087288397042 2316 48-49 -4.281443724829174 2316 50-51 -3.335727102374282 2316 52-53 -4.627922238215703 2316 54-55 -4.689525268994622 2316 56-57 -4.549198656932482 2316 58-59 -3.590388945683131 2316 60-61 -3.1479781439019945 2316 62-63 -2.085583101150121 2316 64-65 -2.2857468702910104 2316 66-67 -2.602111433895864 2316 68-69 -2.578282160710316 2316 70-71 -2.870375595229767 2316 72-73 -3.7176676502987007 2316 74-75 -4.332192980711987 2316 76-77 -3.971759152636608 2316 78-79 -4.10558657345522 2316 80-81 -4.174032747646031 2316 82-83 -3.7700869150275444 2316 84-85 -3.5335971026016004 2316 86-87 -3.5525085294568903 2316 88-89 -3.171507973886289 2316 90-91 -2.449252662183312 2316 92-93 -2.268616512395674 2316 94-95 -1.5456740477106443 2316 96-97 -1.8286474094209453 2316 98-99 -2.059253712575611 2316 100-101 -2.009524294823235 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14656.0 3 4162.0 4 3916.0 5 4612.0 6 5356.0 7 6835.0 8 8748.0 9 10780.0 10 13272.0 11 16317.0 12 19660.0 13 21879.0 14 23933.0 15 27063.0 16 30640.0 17 32075.0 18 29908.0 19 30377.0 20 32367.0 21 35458.0 22 38670.0 23 43940.0 24 48548.0 25 53723.0 26 60202.0 27 68321.0 28 77057.0 29 86270.0 30 98297.0 31 111838.0 32 128452.0 33 146482.0 34 170492.0 35 194901.0 36 201408.0 37 119818.0 38 15447.0 39 99.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.631527585123585 18.31423838882296 13.519262462334583 20.534971563718873 2 13.869431268321147 22.970435941918982 40.352414938012274 22.807717851747597 3 22.348935433633088 27.829053709496343 30.407351475325672 19.414659381544894 4 11.307311806048723 16.231440702031023 41.703245136527634 30.758002355392623 5 11.772102845149025 40.991902336254185 34.406268042210534 12.829726776386257 6 26.525830718443373 38.613828754754884 19.122548033384675 15.737792493417068 7 25.54380123656485 35.97554616259529 21.64854323844607 16.832109362393787 8 22.83366334198325 32.465914820872946 21.319624651031226 23.38079718611258 9 25.602123701583924 20.11392712638228 22.50456702084647 31.77938215118733 10-11 23.291970522911434 25.726771349938133 28.84833483171266 22.132923295437777 12-13 29.477365181337518 23.88922486078344 26.935425600697666 19.69798435718138 14-15 24.863150759459174 25.400734125832436 29.3460455882107 20.390069526497694 16-17 19.838477662544825 28.763474643654067 30.429779371347664 20.96826832245344 18-19 19.1726161912827 31.256789984384113 30.469576073773695 19.101017750559492 20-21 21.758777563704026 28.18617314288287 28.106951366440324 21.948097926972775 22-23 20.36380065155461 27.870877123218335 27.715973570673302 24.04934865455376 24-25 22.252226773363738 27.660996323396386 29.16169154779917 20.925085355440707 26-27 23.439339385829264 25.32961424875714 31.13681566386669 20.09423070154691 28-29 20.422913569211254 27.66934103048696 31.597503117657322 20.31024228264446 30-31 20.34200404920585 29.335721010947623 29.907754413818992 20.414520526027534 32-33 20.562952920956363 28.704706673725607 29.202450544963455 21.529889860354576 34-35 22.306923911954758 26.5328316530127 29.0965398282379 22.063704606794637 36-37 22.881772786876937 26.254100403821027 31.103520755406898 19.760606053895135 38-39 23.450916977230463 25.584149460374388 31.562032954784243 19.40290060761091 40-41 20.98161646742479 27.23074295927828 32.066828540439246 19.720812032857683 42-43 19.63602329143873 29.331431582998018 29.614233700741526 21.418311424821727 44-45 20.778587358838376 28.7879276313442 28.203090144851 22.230394864966424 46-47 21.481609474644678 28.09334246023043 27.047786001338515 23.37726206378638 48-49 22.19794398890963 27.507960372409556 28.278398394732072 22.01569724394874 50-51 22.67958197567991 28.35895519522145 29.19036098072058 19.771101848378052 52-53 21.027017323797896 29.122069331142775 29.574561918671005 20.276351426388324 54-55 19.262414350888683 27.73278215888695 30.60864726723015 22.396156222994218 56-57 21.689489062234617 29.463290117242213 27.45668339679953 21.39053742372364 58-59 22.00460061300499 27.833684075329195 27.867909407931275 22.293805903734548 60-61 21.161128962325762 26.85643472992772 30.042240857042035 21.94019545070448 62-63 23.75610596385023 26.39769665202466 28.902735225720722 20.94346215840439 64-65 22.40084879884727 27.956278591844285 29.553731014228013 20.08914159508043 66-67 20.654986963995032 27.52067366431703 29.656437596355083 22.16790177533285 68-69 19.885350779226435 29.850010690850382 28.99260202995751 21.272036499965676 70-71 21.39061343803915 28.976748546830315 27.191332634299307 22.441305380831228 72-73 20.93566862833416 27.509273001597396 27.686512243807947 23.8685461262605 74-75 20.94828972173617 29.414653998905372 27.791569384749888 21.84548689460857 76-77 22.641477844883408 26.55721035358074 28.526513015838624 22.27479878569723 78-79 21.171004490834054 27.03198485214419 29.824618416974225 21.972392240047526 80-81 21.19924906903025 29.519662696580802 28.274028252238942 21.00705998215 82-83 21.039530653347732 28.525152837761418 28.028819393070435 22.406497115820407 84-85 21.265183650927877 27.51606348806326 27.889951201648334 23.328801659360533 86-87 22.053936199487733 27.85331819079111 28.45409707669186 21.6386485330293 88-89 23.045868976233912 26.23891821986557 28.510472385300485 22.20474041860004 90-91 21.28434826074148 26.729764764051556 29.694499183770812 22.29138779143615 92-93 21.01343467647284 28.931080742186566 28.692062665460092 21.3634219158805 94-95 20.929372587580758 29.180160293702734 27.353652105448973 22.536815013267535 96-97 20.056060777329815 28.01366696933182 27.31633039804777 24.613941855290594 98-99 21.603252536364053 28.094162169165486 26.693395095923567 23.60919019854689 100-101 22.61630602001664 27.655786101651664 27.323053914486177 22.404853963845518 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3889.0 1 2647.5 2 1293.0 3 1014.0 4 835.5 5 873.5 6 1176.0 7 2441.5 8 3361.0 9 2947.5 10 2431.5 11 2200.5 12 2205.0 13 2350.5 14 2424.0 15 2570.0 16 2840.0 17 3027.5 18 3318.0 19 3673.0 20 4161.5 21 4941.0 22 5835.0 23 6835.0 24 8197.0 25 10162.5 26 12918.0 27 15951.5 28 18760.5 29 22200.5 30 25987.0 31 29932.0 32 34851.0 33 40183.5 34 45716.5 35 50485.0 36 55279.5 37 59240.0 38 60043.5 39 59235.5 40 58305.5 41 57799.0 42 57668.0 43 59434.0 44 63018.0 45 69513.5 46 89034.5 47 150692.5 48 199302.5 49 157896.0 50 93893.0 51 63527.0 52 49485.5 53 41630.5 54 35802.5 55 31141.5 56 26794.5 57 23200.0 58 20422.0 59 17328.0 60 13932.5 61 10868.5 62 8237.0 63 6027.0 64 4342.0 65 3079.0 66 2155.5 67 1527.5 68 985.0 69 658.0 70 474.5 71 339.5 72 262.5 73 209.5 74 149.0 75 97.5 76 69.5 77 49.0 78 32.5 79 23.0 80 12.5 81 8.0 82 9.0 83 9.5 84 7.0 85 4.0 86 4.0 87 4.5 88 3.5 89 3.5 90 3.0 91 2.5 92 4.0 93 3.0 94 1.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 52.94121403020365 2 0.08772192640493837 3 0.11925466814736302 4 0.19872503596549868 5 0.12539422066730552 6 0.15078740988978767 7 0.1596774819386644 8 0.157369010191166 9 0.14366552896665438 10-11 0.13118995824613122 12-13 0.14270775877354333 14-15 0.15152415619218076 16-17 0.14477064842024404 18-19 0.15292397416672765 20-21 0.1821973605818135 22-23 0.19987927183924784 24-25 0.2269424193471544 26-27 0.21982053842402108 28-29 0.21638238901285325 30-31 0.23165759568247019 32-33 0.22163784596992406 34-35 0.20989902155179402 36-37 0.19717786873047313 38-39 0.1926345998657157 40-41 0.19366604468906606 42-43 0.18742825932880447 44-45 0.20297360630929887 46-47 0.2269424193471544 48-49 0.22922633288457298 50-51 0.24071957520190534 52-53 0.26761081523925345 54-55 0.25363719370386434 56-57 0.23153480463207135 58-59 0.18951570718558491 60-61 0.19941266584773223 62-63 0.20402960934272898 64-65 0.19307664764715157 66-67 0.20169657938515084 68-69 0.18995775496702078 70-71 0.20272802420850117 72-73 0.22318501320494954 74-75 0.2531705877123487 76-77 0.25461952210705513 78-79 0.24875010989799012 80-81 0.2537599847542632 82-83 0.24486991270538644 84-85 0.21380377695447744 86-87 0.22672139545643644 88-89 0.22890707615353598 90-91 0.21515447850886477 92-93 0.2128460067613664 94-95 0.24960964725078202 96-97 0.2791040575565858 98-99 0.2974244822760942 100-101 0.29312679551213444 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2035979.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.68318363873134 #Duplication Level Percentage of deduplicated Percentage of total 1 83.64409518667469 54.940104644418774 2 10.295135154408584 13.524345058651555 3 3.031018136950073 5.972607627048509 4 1.2040051084103407 3.1633155455074813 5 0.5820692211955638 1.9116079773120773 6 0.32700809961755695 1.2887359835119525 7 0.19975219384255521 0.9184252021280007 8 0.12584225348307218 0.6612575876032325 9 0.09683714056125539 0.572451451788112 >10 0.44972614939960054 5.145012030168112 >50 0.026224988370631367 1.1766389141867128 >100 0.012819605155376543 1.5686073436846761 >500 0.001790835804898045 0.8473535042555633 >1k 0.0030161445135124965 4.534427757155711 >5k 4.7127258023632764E-4 1.9416015635612607 >10k+ 1.8850903209453103E-4 1.8335078090182482 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 16744 0.8224053391513371 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12872 0.6322265602935982 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9130 0.44843291605659974 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6175 0.3032938944851592 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5541 0.2721540841040109 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5263 0.25849971929965876 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5253 0.25800855509806336 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4982 0.24469800523482807 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4363 0.2142949411560728 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4305 0.2114461887868195 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4138 0.20324374662017633 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3778 0.18556183536274196 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3422 0.16807638978594572 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3203 0.15731989377100647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2879 0.14140617363931554 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2674 0.13133730750660982 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2668 0.1310426089856526 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2509 0.12323309818028576 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2490 0.12229988619725449 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2379 0.11684796355954556 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2280 0.11198543796375111 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2124 0.10432327641886287 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2120 0.10412681073822472 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2059 0.10113070910849277 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.4558210079769977E-5 0.0 12-13 0.0 0.0 0.0 1.4734926047861986E-4 0.0 14-15 0.0 0.0 0.0 3.192567310370097E-4 0.0 16-17 0.0 0.0 0.0 5.648388318347094E-4 0.0 18-19 0.0 0.0 0.0 6.630716721537893E-4 0.0 20-21 0.0 0.0 0.0 0.0010560030334301092 0.0 22-23 0.0 0.0 0.0 0.001989215016461368 0.0 24-25 0.0 0.0 0.0 0.003315358360768947 0.0 26-27 0.0 0.0 0.0 0.004739734545395606 0.0 28-29 0.0 0.0 0.0 0.0094057944605519 0.0 30-31 0.0 0.0 0.0 0.020899036777884253 0.0 32-33 0.0 0.0 0.0 0.04678339020196181 0.0 34-35 0.0 0.0 0.0 0.09545776258006591 0.0 36-37 0.0 0.0 0.0 0.158228547543958 0.0 38-39 0.0 0.0 0.0 0.23499751225331894 0.0 40-41 0.0 0.0 0.0 0.3201408265998814 0.0 42-43 0.0 0.0 0.0 0.40835391720641523 0.0 44-45 0.0 0.0 0.0 0.5115966323817682 0.0 46-47 0.0 0.0 0.0 0.6433268712496543 0.0 48-49 0.0 0.0 0.0 0.7845856956284913 0.0 50-51 0.0 0.0 0.0 0.9193120361261093 0.0 52-53 0.0 0.0 0.0 1.0473831016921098 2.4558210079769977E-5 54-55 0.0 0.0 0.0 1.1843933557271464 4.9116420159539954E-5 56-57 0.0 0.0 0.0 1.3526170947735707 4.9116420159539954E-5 58-59 0.0 0.0 0.0 1.5602813192081058 4.9116420159539954E-5 60-61 0.0 0.0 0.0 1.830790003236772 4.9116420159539954E-5 62-63 0.0 0.0 0.0 2.0742846561776913 4.9116420159539954E-5 64-65 0.0 0.0 0.0 2.279591292444568 4.9116420159539954E-5 66-67 0.0 0.0 0.0 2.5141221987063718 4.9116420159539954E-5 68-69 0.0 0.0 0.0 2.781561106475067 4.9116420159539954E-5 70-71 0.0 0.0 0.0 3.0828166695236052 4.9116420159539954E-5 72-73 0.0 0.0 0.0 3.4108897979792525 4.9116420159539954E-5 74-75 0.0 0.0 0.0 3.7417380041739134 4.9116420159539954E-5 76-77 0.0 0.0 0.0 4.045252922549791 4.9116420159539954E-5 78-79 0.0 0.0 0.0 4.4200357665771595 4.9116420159539954E-5 80-81 0.0 0.0 0.0 4.833964397471683 4.9116420159539954E-5 82-83 0.0 0.0 0.0 5.236817275620229 4.9116420159539954E-5 84-85 4.9116420159539954E-5 0.0 0.0 5.747971860220563 4.9116420159539954E-5 86-87 4.9116420159539954E-5 0.0 0.0 6.301661264679057 4.9116420159539954E-5 88-89 4.9116420159539954E-5 0.0 0.0 6.785163304729567 4.9116420159539954E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTATA 2975 0.0 27.31969 1 GTACATG 10095 0.0 26.538883 1 GTATAGG 585 0.0 25.72842 1 TACATGG 11620 0.0 21.95771 2 GAGTACT 7110 0.0 21.90868 12-13 ACATGGG 11240 0.0 21.523014 3 AGTACTT 7545 0.0 21.36502 12-13 ATGGGAG 2500 0.0 21.335867 5 CATGGGA 8570 0.0 21.224457 4 TATACTC 3210 0.0 21.194057 4 ATACTCT 3175 0.0 20.66536 5 TGCACCG 160 5.238523E-5 20.65142 5 ATGGGAT 2770 0.0 20.27865 5 CATTCCG 285 2.1282176E-9 19.874071 9 GTACTTT 7835 0.0 19.82334 14-15 CTTTGCG 645 0.0 19.778961 2 GTACATA 1030 0.0 19.483658 1 TTGCGTT 685 0.0 19.311861 4 GGATATG 580 2.7846909E-8 19.030159 1 TTTGCGT 690 0.0 18.489029 3 >>END_MODULE