FastQCFastQC Report
Fri 27 May 2016
SRR939601_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939601_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413903
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139310.5771151533429472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91070.377272823307316No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT87730.36343631040683905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68670.2844770481663928No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49330.20435783873668495No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39860.16512676772844642No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33280.13786800878080022No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27600.11433765151292327No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG111600.028.402311
TACATGG113700.027.4615362
ACATGGG115750.025.499333
CATGGGA81000.023.727254
GTATCAA242300.022.4817371
GAGTACT101700.021.97471812-13
ATGGGAG31150.021.9372335
AGTACTT104500.021.68105712-13
CGTATAG1805.2194955E-621.0891381
AGAGTAC168150.020.55682610-11
TATCAAC274150.019.611062
TCAACGC275100.019.5958964
ATCAACG276050.019.579223
CAACGCA275950.019.5011435
AACGCAG278250.019.4898016
GTACTTT116350.019.31036414-15
CATGGGG41600.019.2783894
ACGCAGA299650.017.7495637
CGCAGAG299250.017.5361948
GGTATCA186250.017.3242321
Produced by FastQC (version 0.11.5)