##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939601_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2413903 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.315551619099857 31.0 30.0 33.0 26.0 34.0 2 30.425036548693132 31.0 30.0 34.0 26.0 34.0 3 30.458779826695604 31.0 30.0 34.0 26.0 34.0 4 34.06446489357692 35.0 35.0 37.0 28.0 37.0 5 34.070767135216286 35.0 35.0 37.0 30.0 37.0 6 34.1985481603859 36.0 35.0 37.0 30.0 37.0 7 34.007035079702874 36.0 35.0 37.0 29.0 37.0 8 34.02250048987055 36.0 35.0 37.0 29.0 37.0 9 35.30043957855804 38.0 35.0 39.0 29.0 39.0 10-11 35.20330539379586 37.0 35.0 39.0 28.5 39.0 12-13 35.05594031740298 37.0 34.5 39.0 27.0 39.0 14-15 35.720113442835114 38.0 34.0 40.0 27.0 41.0 16-17 35.48194915040082 38.0 34.0 40.0 27.0 41.0 18-19 35.50939142956449 38.0 34.0 40.0 27.0 41.0 20-21 35.64591162113805 38.0 34.0 40.0 27.0 41.0 22-23 35.49372178583812 38.0 34.0 40.0 27.0 41.0 24-25 35.366972906533526 38.0 34.0 40.0 27.0 41.0 26-27 35.46829263644811 38.0 34.0 40.0 27.0 41.0 28-29 35.34263659310254 38.0 34.0 40.0 26.0 41.0 30-31 35.097212274064034 38.0 34.0 40.0 25.0 41.0 32-33 34.83846989709197 38.0 34.0 40.0 25.0 41.0 34-35 34.58015359357853 38.0 33.0 40.0 24.0 41.0 36-37 34.27382811156869 38.0 33.0 40.0 24.0 41.0 38-39 34.09131104273867 38.0 33.0 40.0 23.5 41.0 40-41 33.81331188535745 38.0 33.0 40.0 22.0 41.0 42-43 33.46797882930673 38.0 32.0 40.0 19.5 41.0 44-45 33.14903125767688 37.0 31.5 40.0 17.0 41.0 46-47 32.63477964938939 37.0 31.0 40.0 14.0 41.0 48-49 32.22818025413614 37.0 30.5 40.0 10.0 41.0 50-51 31.58749295228516 36.0 30.0 38.5 9.0 40.5 52-53 31.63171511034205 36.5 30.0 39.0 8.0 40.0 54-55 31.67776957069112 36.5 30.0 39.0 7.5 40.0 56-57 31.52839198592487 36.0 30.0 39.0 6.5 40.0 58-59 31.132658188833602 36.0 29.0 39.0 2.0 40.0 60-61 30.699356809283554 35.0 28.5 39.0 2.0 40.0 62-63 30.301209700638346 35.0 28.0 38.5 2.0 40.0 64-65 29.697538384930958 34.5 27.5 38.0 2.0 40.0 66-67 29.14698933635693 34.0 26.0 38.0 2.0 40.0 68-69 28.633399105100743 34.0 26.0 37.0 2.0 39.0 70-71 28.075317856599874 33.5 26.0 36.5 2.0 39.0 72-73 27.33111293204408 33.0 24.5 36.0 2.0 38.5 74-75 26.88326850747524 32.0 24.0 35.0 2.0 38.0 76-77 26.51256409226054 32.0 22.5 35.0 2.0 37.5 78-79 25.928543939006666 32.0 21.5 35.0 2.0 37.0 80-81 25.25190925236018 31.0 19.0 34.5 2.0 36.0 82-83 24.590353050640395 31.0 17.0 34.0 2.0 36.0 84-85 23.927726797638513 30.5 9.5 34.0 2.0 35.0 86-87 23.27836081234416 30.0 4.5 34.0 2.0 35.0 88-89 22.495194504501633 29.0 2.0 34.0 2.0 35.0 90-91 21.261115297507814 28.0 2.0 33.5 2.0 35.0 92-93 19.613322697722317 25.5 2.0 33.0 2.0 34.0 94-95 19.369308128785622 25.5 2.0 32.0 2.0 34.0 96-97 18.741599807448765 25.0 2.0 32.0 2.0 34.0 98-99 17.763808653454593 24.5 2.0 32.0 2.0 34.0 100-101 16.117867826503385 17.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9202712279762686 1101 2 0.9111688083297906 1101 3 0.929264666276886 1101 4 0.9551436034761238 1101 5 0.902297577418409 1101 6 0.7684493399445813 1101 7 0.9016358113788456 1101 8 0.9080386436567949 1101 9 1.0991636834564176 1101 10-11 1.0371971079827063 1101 12-13 0.8967877141964777 1101 14-15 1.0247755983478086 1101 16-17 1.0157111262104621 1101 18-19 1.0319002673410722 1101 20-21 1.119782613440286 1101 22-23 1.2027937786356873 1101 24-25 0.941613468284757 1101 26-27 1.0752309670952442 1101 28-29 1.33826465713355 1101 30-31 1.1918529042479378 1101 32-33 1.3108496552500384 1101 34-35 1.466058725762501 1101 36-37 1.3612075941662596 1101 38-39 1.2153613364974802 1101 40-41 1.484715091568546 1101 42-43 1.4435246480585846 1101 44-45 1.242916307794303 1101 46-47 1.525821374466787 1101 48-49 1.4644732004275411 1101 50-51 1.368661859156969 1101 52-53 1.2353378588624793 1101 54-55 1.2118245226327389 1101 56-57 1.2617946292683833 1101 58-59 1.3107765084093295 1101 60-61 1.2908173709380328 1101 62-63 1.3702992041236648 1101 64-65 1.2005804252745058 1101 66-67 1.237952259755108 1101 68-69 1.3005393214822973 1101 70-71 1.019843489961687 1101 72-73 1.1031043857979128 1101 74-75 0.6802716118924934 1101 76-77 0.33391075542271054 1101 78-79 0.7888641199685296 1101 80-81 0.5689164131815687 1101 82-83 0.5800671188161566 1101 84-85 0.15119608142543228 1101 86-87 -0.4958309135410168 1101 88-89 -0.8092771746734755 1101 90-91 -3.7360746969660994 1101 92-93 -8.984062406011427 1101 94-95 -7.374572930507426 1101 96-97 -4.006964187543829 1101 98-99 -2.8583753391072158 1101 100-101 -2.3144336819334335 1102 1 1.056066254495697 1102 2 1.0199566423889799 1102 3 0.8934531577666647 1102 4 1.141738867310714 1102 5 1.027094939789798 1102 6 0.9615576999482371 1102 7 1.0345450549064736 1102 8 0.9022269087981485 1102 9 1.092187688822463 1102 10-11 1.0097325960680195 1102 12-13 1.0244781919733583 1102 14-15 1.1471381211699452 1102 16-17 1.3052096433094889 1102 18-19 1.3005525728910499 1102 20-21 1.1584333600553336 1102 22-23 1.2601230521497016 1102 24-25 1.2681245751381098 1102 26-27 1.204488349799739 1102 28-29 1.3035754861203017 1102 30-31 1.2244016480620061 1102 32-33 1.1383651433799713 1102 34-35 1.1058766969983935 1102 36-37 1.0802747708713767 1102 38-39 0.9720677055276639 1102 40-41 1.1814565031602058 1102 42-43 1.2090007432741174 1102 44-45 1.283592553993799 1102 46-47 1.1822284534977783 1102 48-49 1.241403322843773 1102 50-51 1.2795282412197189 1102 52-53 1.3626907268085802 1102 54-55 1.2350569566402214 1102 56-57 1.3446552078850864 1102 58-59 1.2020948349484932 1102 60-61 1.2091653653794232 1102 62-63 1.110914431889153 1102 64-65 1.1495887482016585 1102 66-67 1.160511928865592 1102 68-69 0.8544749626556474 1102 70-71 0.8696332569088874 1102 72-73 0.9545559008499254 1102 74-75 0.9747485190931471 1102 76-77 0.6821444566625594 1102 78-79 0.4254775076169821 1102 80-81 0.4243688757089501 1102 82-83 0.5429058085839635 1102 84-85 0.050397868494052034 1102 86-87 -0.21017633462400198 1102 88-89 -0.6363895394502208 1102 90-91 -4.606291133878489 1102 92-93 -9.929296926887606 1102 94-95 -8.461721377135584 1102 96-97 -4.880166989035946 1102 98-99 -3.6103224934452793 1102 100-101 -2.9394428873006326 1103 1 0.8109721667119416 1103 2 0.9188942194573748 1103 3 0.8511237716948337 1103 4 0.8499521366228535 1103 5 0.7812002633321882 1103 6 0.48443452939695675 1103 7 0.7424613003171743 1103 8 0.7790050460261071 1103 9 0.9090689734678321 1103 10-11 0.8005096073568723 1103 12-13 0.7594058071676528 1103 14-15 1.0459810876277729 1103 16-17 1.0174561249116536 1103 18-19 0.9857803198117878 1103 20-21 1.0047783635953422 1103 22-23 0.964491601996464 1103 24-25 0.896880389765407 1103 26-27 0.9706559191924597 1103 28-29 0.912518106929717 1103 30-31 0.9941906429298868 1103 32-33 0.9412546598768614 1103 34-35 1.1936392475126922 1103 36-37 1.1073578472426036 1103 38-39 1.0514429323422405 1103 40-41 0.9588938230237005 1103 42-43 0.8916694714647164 1103 44-45 0.8180833417206088 1103 46-47 0.9977274844067168 1103 48-49 0.7666495451367936 1103 50-51 0.5935508124463773 1103 52-53 0.9440784321880642 1103 54-55 0.9669227167284333 1103 56-57 0.8580980888129766 1103 58-59 0.7803968909366077 1103 60-61 0.5007498446423 1103 62-63 0.7935579899344241 1103 64-65 0.6144671691157662 1103 66-67 0.5696813723415382 1103 68-69 0.647641357225762 1103 70-71 0.758008275563931 1103 72-73 0.8392692703570468 1103 74-75 0.589563634259104 1103 76-77 0.32275071562790814 1103 78-79 0.36687469186277255 1103 80-81 0.3309669177144414 1103 82-83 0.35688119590475154 1103 84-85 0.2419896155352248 1103 86-87 -0.08283110026323826 1103 88-89 -0.3283997098075915 1103 90-91 -1.7925282764664736 1103 92-93 -8.865463900613857 1103 94-95 -5.184959604548737 1103 96-97 -2.592254275538888 1103 98-99 -1.7565157153436601 1103 100-101 -1.272605694922703 1104 1 0.8312937513498575 1104 2 0.7596793764661349 1104 3 0.7949024686490382 1104 4 0.8279213962488825 1104 5 0.743642631089358 1104 6 0.744529183913329 1104 7 0.9483542381106957 1104 8 0.7789898522065926 1104 9 0.9761133406124785 1104 10-11 0.8670101251384068 1104 12-13 0.8389286199428483 1104 14-15 0.9236596686322827 1104 16-17 0.9084824763572144 1104 18-19 0.7978576486072342 1104 20-21 0.916036179474851 1104 22-23 0.8635942133075787 1104 24-25 0.8046200916328772 1104 26-27 0.7444053486794289 1104 28-29 0.8262806686434345 1104 30-31 0.83346848366768 1104 32-33 0.9767955360180309 1104 34-35 0.839380151777334 1104 36-37 0.8221700039665549 1104 38-39 0.6301168276919711 1104 40-41 0.710416035196161 1104 42-43 0.7857538025255266 1104 44-45 0.7156287354907889 1104 46-47 0.6099457582219827 1104 48-49 0.50253984933493 1104 50-51 0.6006233864700725 1104 52-53 0.6098476207904326 1104 54-55 0.8695721911414438 1104 56-57 0.9169970791091906 1104 58-59 0.8200999798699051 1104 60-61 0.6977083696858841 1104 62-63 0.47663025769838185 1104 64-65 0.5963270892499004 1104 66-67 0.9828053467587239 1104 68-69 0.6743179803129777 1104 70-71 0.7583529667242281 1104 72-73 0.7846016098358355 1104 74-75 0.7388990685103849 1104 76-77 0.7786738921014376 1104 78-79 0.49915938352356903 1104 80-81 0.44333742764285944 1104 82-83 0.25293148804317056 1104 84-85 0.17434479460183638 1104 86-87 0.20958631983812026 1104 88-89 -0.036680162690664986 1104 90-91 -0.6728492414348892 1104 92-93 -3.987569031119829 1104 94-95 -1.3611368574346692 1104 96-97 -0.8356603068298121 1104 98-99 -0.6522671956983501 1104 100-101 -0.637237849573145 1105 1 0.7929998324101639 1105 2 0.690072934238728 1105 3 0.7244693509666078 1105 4 0.8086649922041076 1105 5 0.6351495476175799 1105 6 0.5679757165493342 1105 7 0.7859630831998956 1105 8 0.745611408852767 1105 9 0.9639862053672701 1105 10-11 0.6707878382878576 1105 12-13 0.7164968783107497 1105 14-15 0.938387803389297 1105 16-17 1.0600198652064066 1105 18-19 0.934215290113535 1105 20-21 0.758568339578737 1105 22-23 0.7066851248579198 1105 24-25 0.8490200033393975 1105 26-27 0.8668138794536446 1105 28-29 0.8474658796070784 1105 30-31 0.8862009396408368 1105 32-33 1.084665308041295 1105 34-35 0.9646898860729607 1105 36-37 0.8550757758766849 1105 38-39 0.8898709217183907 1105 40-41 0.7689044764963597 1105 42-43 0.924852477262398 1105 44-45 0.6964860800210815 1105 46-47 0.6954333461004367 1105 48-49 0.8516575907924135 1105 50-51 1.0209717153497841 1105 52-53 0.8791564414083126 1105 54-55 0.9213423956552305 1105 56-57 0.8041418914978316 1105 58-59 0.7302956335675965 1105 60-61 0.6847344224135732 1105 62-63 0.6423030589999108 1105 64-65 0.8106895069666393 1105 66-67 0.876042625056602 1105 68-69 0.7036987761103042 1105 70-71 0.8299721438731815 1105 72-73 1.0702501058337255 1105 74-75 0.9212083674871359 1105 76-77 0.7688351015008799 1105 78-79 0.5890261378278758 1105 80-81 0.357818174620526 1105 82-83 0.31032394645115957 1105 84-85 0.607850605616882 1105 86-87 0.36292908303080296 1105 88-89 -0.044453801796560555 1105 90-91 -1.270578635419369 1105 92-93 -7.139853625135794 1105 94-95 -2.817501828038349 1105 96-97 -0.9068765988230219 1105 98-99 -0.4215329763148574 1105 100-101 -0.3441617253375657 1106 1 0.8362885938068523 1106 2 0.8538328320199469 1106 3 0.8636694943287146 1106 4 0.9437101659240739 1106 5 0.8218900182343418 1106 6 0.6747898134679815 1106 7 0.7977771334858872 1106 8 0.816045705439123 1106 9 0.8615329279575974 1106 10-11 0.9444697329593481 1106 12-13 0.9604144810724691 1106 14-15 0.9726846229089219 1106 16-17 1.1727216680248844 1106 18-19 1.0039764110026965 1106 20-21 1.018392507818696 1106 22-23 0.8825478623014078 1106 24-25 0.896315675695682 1106 26-27 0.8890962948120418 1106 28-29 0.9672240359916842 1106 30-31 1.0565695174913898 1106 32-33 1.0545609308337376 1106 34-35 0.996425406966388 1106 36-37 1.27288588636943 1106 38-39 0.997807200192625 1106 40-41 1.0554791347123 1106 42-43 1.1569801787920895 1106 44-45 1.0015019551095108 1106 46-47 1.304674839304731 1106 48-49 1.088204902286563 1106 50-51 1.1080133996147303 1106 52-53 1.06557281506646 1106 54-55 1.1506528185867708 1106 56-57 1.1668903269722186 1106 58-59 1.0828530017270843 1106 60-61 1.1352313599804411 1106 62-63 1.1110218734112642 1106 64-65 1.035686409225928 1106 66-67 0.9140357487383959 1106 68-69 0.8956389544069374 1106 70-71 0.8859291658851305 1106 72-73 0.9310587893672029 1106 74-75 1.1125932840879642 1106 76-77 0.8594974784073237 1106 78-79 0.8281392446686198 1106 80-81 0.7712578059827209 1106 82-83 0.939608821349065 1106 84-85 0.7017196437983664 1106 86-87 0.7319865837602713 1106 88-89 0.7292902142344104 1106 90-91 0.34142467683155786 1106 92-93 -3.2222195529191318 1106 94-95 -0.8958248710989452 1106 96-97 -0.0627221021692499 1106 98-99 -0.1196016848700836 1106 100-101 0.055280947806153335 1107 1 0.5824835760982126 1107 2 0.5485589851619963 1107 3 0.5848897003834601 1107 4 0.5265663280634669 1107 5 0.3296034190737487 1107 6 0.5121719508828875 1107 7 0.45916826304241454 1107 8 0.46844079278616135 1107 9 0.33411378702176364 1107 10-11 0.3627200359045446 1107 12-13 0.5974142964449243 1107 14-15 0.6548531774572623 1107 16-17 0.6373206361909354 1107 18-19 0.43758135486032756 1107 20-21 0.5429304188750308 1107 22-23 0.6718410248412638 1107 24-25 0.6435758419124866 1107 26-27 0.5609299323907706 1107 28-29 0.6608710281229122 1107 30-31 0.6582570392696354 1107 32-33 0.519496324194435 1107 34-35 0.5370122625252378 1107 36-37 0.5430765988237383 1107 38-39 0.4828185638880882 1107 40-41 0.5114977976968191 1107 42-43 0.37228580239112574 1107 44-45 0.14501086663911877 1107 46-47 0.109926257816376 1107 48-49 0.20018996045724435 1107 50-51 0.2785006863179085 1107 52-53 0.3621834693760846 1107 54-55 0.3192200438167774 1107 56-57 0.1240547859094967 1107 58-59 0.19417236057542553 1107 60-61 0.1466035067047322 1107 62-63 0.15934455815582993 1107 64-65 0.36760773188726503 1107 66-67 0.29598756094877743 1107 68-69 0.2587961374586456 1107 70-71 -0.04939463442587666 1107 72-73 0.30502123456402686 1107 74-75 0.26120903255763395 1107 76-77 0.19084346522856066 1107 78-79 0.18819871554167733 1107 80-81 0.06617528267024397 1107 82-83 -0.0829800989178402 1107 84-85 -0.2953971760311518 1107 86-87 -0.25709438712058486 1107 88-89 -0.4348043436736262 1107 90-91 -0.4874021841360978 1107 92-93 -3.8535196428925556 1107 94-95 -1.6478330207148666 1107 96-97 -0.8859506528690098 1107 98-99 -0.7088258721188225 1107 100-101 -0.5056068116475565 1108 1 0.2907967420661386 1108 2 0.2512297374996315 1108 3 -0.03570106106507964 1108 4 -0.27105593955510443 1108 5 -0.9727976244094663 1108 6 -0.23650115510492498 1108 7 -0.029818872551089726 1108 8 0.10872409681749673 1108 9 0.218737179267535 1108 10-11 0.2502587987671703 1108 12-13 0.2475112480883297 1108 14-15 0.29800484434932883 1108 16-17 0.2811338223262254 1108 18-19 0.08787894213163838 1108 20-21 0.10264628442276802 1108 22-23 0.003631554720989527 1108 24-25 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60-61 0.38703002241883766 2311 62-63 0.3795384915629576 2311 64-65 0.5067642050525514 2311 66-67 0.3743487892131512 2311 68-69 0.3622105517468306 2311 70-71 0.37665052056672366 2311 72-73 0.25882645812047045 2311 74-75 0.45595540936922774 2311 76-77 0.4646277179620064 2311 78-79 0.39133896784543154 2311 80-81 0.36400796679546943 2311 82-83 0.3407274398361473 2311 84-85 0.41925017319143265 2311 86-87 0.522734156551504 2311 88-89 0.6012450965595306 2311 90-91 1.4611941952443068 2311 92-93 2.771089197547038 2311 94-95 1.8724915132798188 2311 96-97 1.375874334170181 2311 98-99 1.2861226232596579 2311 100-101 1.5517205410312478 2312 1 0.22421644953097442 2312 2 -0.02959780664728129 2312 3 -0.1353875995627476 2312 4 -0.12577455402346516 2312 5 0.014730394193009033 2312 6 0.14390627258865152 2312 7 0.14331159477968924 2312 8 -0.4936299716064241 2312 9 0.009042851200952384 2312 10-11 0.1394709684150257 2312 12-13 0.26035912315462895 2312 14-15 -0.08171431206492485 2312 16-17 0.23966166606082595 2312 18-19 0.2997266018647622 2312 20-21 0.4217665689515826 2312 22-23 0.22930938450951288 2312 24-25 0.5079665771031543 2312 26-27 0.31115012900121997 2312 28-29 0.23941575020227646 2312 30-31 0.4298262578524614 2312 32-33 0.30751430346934683 2312 34-35 0.27010323180411433 2312 36-37 0.35314401741009505 2312 38-39 0.43448407784766374 2312 40-41 0.5452365735810076 2312 42-43 0.382773959064707 2312 44-45 0.36327743163110426 2312 46-47 0.5052080651428597 2312 48-49 0.5352715662077756 2312 50-51 0.40055877093251624 2312 52-53 0.37662127343952534 2312 54-55 0.39804695745832674 2312 56-57 0.32294390139892215 2312 58-59 0.3814364250797304 2312 60-61 0.5879191888413224 2312 62-63 0.4688340714769872 2312 64-65 0.5783574644001099 2312 66-67 0.6330787932813244 2312 68-69 0.5083765816351118 2312 70-71 0.5983439907196875 2312 72-73 0.9161404244769535 2312 74-75 0.8910082102932471 2312 76-77 0.9567830397318637 2312 78-79 0.8205043661709333 2312 80-81 0.7932431357385141 2312 82-83 1.0838710008829509 2312 84-85 1.2706730386088196 2312 86-87 1.046283340213943 2312 88-89 1.369167397268754 2312 90-91 1.9117279653611448 2312 92-93 3.0115660416676704 2312 94-95 2.363865773967312 2312 96-97 2.007585542133558 2312 98-99 1.8563999845789994 2312 100-101 1.8189818344831927 2313 1 -0.20389195163245688 2313 2 -0.9213871291766402 2313 3 -0.39180294686993733 2313 4 -0.15996165519349148 2313 5 -0.7992629974094925 2313 6 -0.42974219290042015 2313 7 -1.120473361225379 2313 8 -1.155827300407168 2313 9 -1.1927770223456804 2313 10-11 -2.089816980417517 2313 12-13 -0.5497538496718235 2313 14-15 -0.1536399785826248 2313 16-17 -1.5263501440686298 2313 18-19 -0.9371690631143963 2313 20-21 -1.401804603196446 2313 22-23 -1.0964241695921615 2313 24-25 -0.2803408818874189 2313 26-27 -0.7056910276881325 2313 28-29 -2.194991142458882 2313 30-31 -0.39279541505384685 2313 32-33 -0.1798242413644573 2313 34-35 -0.04267837604390934 2313 36-37 -0.007329047142661693 2313 38-39 -0.3093698194217893 2313 40-41 -0.07277387384120715 2313 42-43 0.22221664110701056 2313 44-45 -0.32430618071531825 2313 46-47 -0.4063159690566991 2313 48-49 0.12211228331414503 2313 50-51 0.06163736104336692 2313 52-53 0.09957446517829283 2313 54-55 0.2686644982845934 2313 56-57 -0.07295955152540401 2313 58-59 -0.0256155849134565 2313 60-61 -0.13773597296184548 2313 62-63 0.025489357828668346 2313 64-65 0.058005138639813936 2313 66-67 0.023421887798992458 2313 68-69 0.010537498312157112 2313 70-71 -0.09305043976392469 2313 72-73 0.12746737897278848 2313 74-75 0.07789178674039121 2313 76-77 0.1199826177321981 2313 78-79 0.3610694500946501 2313 80-81 0.4401891329691381 2313 82-83 0.11284977635128968 2313 84-85 0.4603516301849453 2313 86-87 0.374484053803652 2313 88-89 0.6743885441407542 2313 90-91 1.2205507621346072 2313 92-93 2.3306388146303867 2313 94-95 1.7720896389274188 2313 96-97 1.2984975302941244 2313 98-99 1.0125775907566066 2313 100-101 0.8963000852359748 2314 1 -0.5117496895331115 2314 2 -0.5775396531403238 2314 3 -1.6206724997738249 2314 4 -0.4515924811590821 2314 5 -0.3789444457604674 2314 6 -2.9367020929933645 2314 7 -3.8247433633494587 2314 8 -1.48845858282386 2314 9 -1.817231653667264 2314 10-11 -1.0841221880274503 2314 12-13 -1.9978961087582832 2314 14-15 -2.8442425844212735 2314 16-17 -2.1775263718542988 2314 18-19 -2.447638062853777 2314 20-21 -2.160589629792625 2314 22-23 -3.3339133420598728 2314 24-25 -1.7858087292007596 2314 26-27 -1.9733562125206987 2314 28-29 -1.2656104547090976 2314 30-31 -1.397463719722154 2314 32-33 -2.2470822356610753 2314 34-35 -0.9337075301631899 2314 36-37 -0.37533837802752856 2314 38-39 -0.5775450092707999 2314 40-41 -0.7153702520598983 2314 42-43 -0.579477123891472 2314 44-45 -1.1693197160722022 2314 46-47 -1.507455063114243 2314 48-49 -1.9236952961844196 2314 50-51 -1.0526563373705038 2314 52-53 -0.6436994341041888 2314 54-55 -0.2615944435623305 2314 56-57 -0.5013338625177433 2314 58-59 -0.5869301490177179 2314 60-61 -0.48036602009833373 2314 62-63 -0.5582905845349657 2314 64-65 -0.7421029483781432 2314 66-67 -0.4056719739128951 2314 68-69 -0.2024096693560402 2314 70-71 -0.30826487386248047 2314 72-73 -0.18306406267453568 2314 74-75 -0.1855249380497277 2314 76-77 -0.20544909010294177 2314 78-79 -0.15031041815216994 2314 80-81 -0.13073493194634267 2314 82-83 0.140674764262112 2314 84-85 -0.10309672982993945 2314 86-87 -0.041401498991771035 2314 88-89 0.2842030713372594 2314 90-91 0.9144441845688007 2314 92-93 1.8624585782098109 2314 94-95 1.1162573839535348 2314 96-97 0.828240742655364 2314 98-99 0.7338873041264051 2314 100-101 0.6553871529067372 2315 1 -7.381934123312799 2315 2 -3.2089052636132642 2315 3 -1.500785345911975 2315 4 -0.9606793567064216 2315 5 -1.3860572895715748 2315 6 -1.7926074722666954 2315 7 -3.1997797622017607 2315 8 -2.992732510075797 2315 9 -3.374483693515568 2315 10-11 -2.3583217817255004 2315 12-13 -2.9130723940042813 2315 14-15 -4.8009058044766775 2315 16-17 -4.773295882763875 2315 18-19 -3.505306953916296 2315 20-21 -4.344819870664988 2315 22-23 -5.754216028477458 2315 24-25 -3.81989698158268 2315 26-27 -4.572540893742769 2315 28-29 -3.13108270121446 2315 30-31 -2.9162600327530583 2315 32-33 -1.9820356831385197 2315 34-35 -2.8290796594630834 2315 36-37 -3.2487780366035786 2315 38-39 -2.5437999183648117 2315 40-41 -3.6659200162915226 2315 42-43 -7.574714129481812 2315 44-45 -4.486587703931235 2315 46-47 -3.0039076391135744 2315 48-49 -6.005969035721449 2315 50-51 -6.8674268636471005 2315 52-53 -7.089437025721864 2315 54-55 -4.852490425454821 2315 56-57 -4.224785660508569 2315 58-59 -4.531766709056626 2315 60-61 -3.5183200361502855 2315 62-63 -3.6852550559972563 2315 64-65 -2.530880466647382 2315 66-67 -3.1569929206476246 2315 68-69 -2.606232537841578 2315 70-71 -2.355690813736306 2315 72-73 -2.7473858184450215 2315 74-75 -3.1795381510076375 2315 76-77 -3.2580618567337503 2315 78-79 -2.3411247207873913 2315 80-81 -2.119716299302098 2315 82-83 -2.5493377164525057 2315 84-85 -2.3942520220330223 2315 86-87 -2.832402042744473 2315 88-89 -2.2441285386904397 2315 90-91 -1.4026778200402958 2315 92-93 -0.628766913165002 2315 94-95 -0.8006937380309083 2315 96-97 -0.8637601974705582 2315 98-99 -0.8386298364945617 2315 100-101 -0.6078526048014048 2316 1 -4.189643247387938 2316 2 -2.7979588034042386 2316 3 -4.421741484343006 2316 4 -4.306984447883927 2316 5 -3.9331394679121665 2316 6 -4.105415929185288 2316 7 -2.7791845390658985 2316 8 -1.633143539087996 2316 9 -1.8337227339485693 2316 10-11 -2.9561527115920967 2316 12-13 -4.094190707147607 2316 14-15 -3.0849673622959344 2316 16-17 -2.7389510934004235 2316 18-19 -3.2436081638188767 2316 20-21 -5.522395438605191 2316 22-23 -6.528700723488637 2316 24-25 -4.261594972789894 2316 26-27 -3.847206920187702 2316 28-29 -4.885415723590874 2316 30-31 -6.126582027115887 2316 32-33 -4.548114555238698 2316 34-35 -6.154064701076109 2316 36-37 -9.041533517115234 2316 38-39 -5.936262139595819 2316 40-41 -5.241314530745193 2316 42-43 -4.495896703356273 2316 44-45 -3.4319788147797468 2316 46-47 -5.577085066803608 2316 48-49 -3.1937159739331165 2316 50-51 -4.689915040488106 2316 52-53 -5.498338992739349 2316 54-55 -6.6852025661726735 2316 56-57 -4.793896487384902 2316 58-59 -5.647214499501068 2316 60-61 -6.243115923862938 2316 62-63 -6.46484948606815 2316 64-65 -5.109934064690766 2316 66-67 -6.350580578459645 2316 68-69 -5.260426669826561 2316 70-71 -5.841542967452771 2316 72-73 -7.629579720749071 2316 74-75 -7.590550769312042 2316 76-77 -6.78750811763631 2316 78-79 -5.857486075472178 2316 80-81 -6.11686176472638 2316 82-83 -5.1404345011690715 2316 84-85 -6.483507531254794 2316 86-87 -4.980582362250626 2316 88-89 -4.688294102028706 2316 90-91 -5.001644838357354 2316 92-93 -3.906065449281913 2316 94-95 -3.7425920343143204 2316 96-97 -4.695152338554571 2316 98-99 -5.705341329778474 2316 100-101 -4.6091791786359195 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11378.0 3 4292.0 4 4610.0 5 5272.0 6 6129.0 7 7472.0 8 9218.0 9 11523.0 10 13950.0 11 17450.0 12 20739.0 13 24153.0 14 26259.0 15 29881.0 16 33970.0 17 38134.0 18 34152.0 19 30601.0 20 32285.0 21 34605.0 22 38314.0 23 43073.0 24 48386.0 25 54457.0 26 61633.0 27 70166.0 28 80015.0 29 90992.0 30 104340.0 31 120105.0 32 138466.0 33 160878.0 34 190079.0 35 230255.0 36 268016.0 37 240875.0 38 76525.0 39 1255.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.82105924831284 19.22551609869688 13.615601630021047 22.337823022969232 2 15.160452256201108 21.409392215935128 40.185530372615816 23.24462515524795 3 19.095956031452076 26.574449045465514 30.54958196871545 23.78001295436696 4 12.097478484220932 16.949217204661355 38.77451496711986 32.17878934399785 5 12.678130844221814 38.632757912894924 34.57722562480044 14.111885618082825 6 29.923548288128426 37.501140142123894 18.166941682766854 14.408369886980822 7 26.60687178156056 34.22153519794634 22.093105703098534 17.078487317394572 8 25.704691085287713 34.138303633105366 20.754583985345178 19.402421296261743 9 26.88987934823168 16.290808076620092 21.134168083253865 35.685144491894356 10-11 24.31719258091367 26.694405831444147 28.30541276519875 20.68298882244343 12-13 26.13328451724105 24.754858769875938 28.244677256109924 20.867179456773087 14-15 22.91118455152739 25.491721366876348 27.59384731488661 24.00324676670965 16-17 21.310778331259076 28.883119801783348 27.804484338773676 22.001617528183907 18-19 21.17895754712874 27.500716317823688 30.49359792240878 20.82672821263879 20-21 22.500361307108555 26.321051794540356 30.150533915488765 21.028052982862327 22-23 21.84083736975716 26.600665486326342 30.101788800370276 21.45670834354622 24-25 21.93472980737121 26.59785239743715 30.305816613740593 21.161601181451054 26-27 21.698929428488515 26.52099210870706 30.635777719558842 21.144300743245584 28-29 21.2455293673015 27.260643538787104 30.406442274543416 21.087384819367976 30-31 21.601160298363624 26.673773328197285 30.284832753868677 21.44023361957041 32-33 21.40867057370585 26.919512420758835 30.195132089701772 21.476684915833548 34-35 22.07438798867363 26.70488721336664 30.046340044917198 21.17438475304253 36-37 21.979916791498777 26.403847885003177 30.198965307389823 21.417270016108215 38-39 21.571408304994893 27.061128897244085 30.198420237420677 21.169042560340344 40-41 21.711310465117485 26.923388815320788 30.079878714598735 21.285422004962996 42-43 21.438567936015716 27.172498239204394 29.439818121635515 21.94911570314438 44-45 21.78195831595686 26.872547895298617 29.448669292199114 21.89682449654541 46-47 22.119326946391464 27.1738871162748 29.10063094368176 21.606154993651984 48-49 22.06211415671186 27.253673329756783 28.883804819879984 21.800407693651373 50-51 21.49203887137085 27.618192810730086 29.104459651180115 21.78530866671895 52-53 21.922191791736793 27.961081163630965 28.31548484727131 21.80124219736094 54-55 21.526451594697978 27.359969590812177 28.682466331202868 22.43111248328698 56-57 21.919513069335846 27.708078893918675 28.284280456397937 22.088127580347543 58-59 22.34118454226354 27.267630791030523 28.383236116169464 22.007948550536472 60-61 22.015956708784152 27.49980245437733 28.88409433161242 21.600146505226093 62-63 21.645292688728695 27.452072647122076 28.86292732488025 22.03970733926898 64-65 21.45424572317263 27.85509590461379 28.65528252980819 22.03537584240539 66-67 21.670884259330307 27.74096169408275 28.060111998135163 22.528042048451784 68-69 21.587744136959337 27.537118523363606 28.478411177469198 22.39672616220785 70-71 21.88368299504414 27.54526797834313 28.288843974933446 22.28220505167928 72-73 21.86814212717756 27.29207769818202 28.47732143338748 22.36245874125294 74-75 21.50812035117365 28.165538471242634 27.982241296600307 22.344099880983407 76-77 21.744519999493704 27.507974710834382 28.094180541269814 22.653324748402103 78-79 21.541722387468187 27.794763472330146 28.10121145408727 22.562302686114393 80-81 21.858721881256923 27.695214996967316 27.933347734180288 22.512715387595474 82-83 21.764195057473295 27.62851472081956 28.174767777606014 22.432522444101142 84-85 21.632969408896457 27.632608852145385 27.932464276771498 22.80195746218666 86-87 21.644633673086012 27.588116933674478 28.00070784401956 22.76654154921995 88-89 21.699057340404714 27.538550189583955 27.64528907791835 23.11710339209298 90-91 21.523283897301777 27.31979083499011 28.126508441456476 23.03041682625164 92-93 21.4572515547616 27.610703858741658 28.409123101124496 22.522921485372244 94-95 21.750144160448766 27.27034291200579 27.883670662212616 23.09584226533283 96-97 21.529002181513913 27.235408427882206 28.008899547427173 23.22668984317671 98-99 21.64370851992341 27.413220696060982 27.385178996746458 23.557891787269153 100-101 21.586417637592078 27.134127925308714 27.773258359188812 23.506196077910392 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3559.0 1 2283.5 2 1121.0 3 1160.0 4 992.0 5 1033.0 6 1466.5 7 2501.5 8 3918.0 9 4507.5 10 3963.5 11 3426.0 12 3389.5 13 3574.5 14 3882.5 15 4146.0 16 4334.0 17 4654.0 18 5070.0 19 5479.0 20 6029.5 21 7020.0 22 8312.5 23 9761.5 24 11172.5 25 13197.0 26 16586.5 27 20346.5 28 23479.5 29 27046.0 30 31509.5 31 36247.5 32 40906.0 33 45186.0 34 50280.0 35 55483.0 36 60800.5 37 66071.5 38 68554.5 39 68463.0 40 68429.5 41 70830.0 42 73528.0 43 75973.5 44 79215.0 45 81339.0 46 83744.0 47 90779.0 48 104068.0 49 104974.5 50 92722.0 51 84935.5 52 79822.0 53 76625.5 54 73562.5 55 68410.5 56 62975.0 57 56951.0 58 50733.5 59 44447.0 60 37510.0 61 30677.0 62 24704.5 63 19200.5 64 13960.5 65 10021.0 66 7266.5 67 5334.5 68 3742.0 69 2449.0 70 1718.0 71 1196.5 72 829.0 73 606.5 74 460.0 75 332.5 76 221.5 77 146.0 78 96.0 79 68.0 80 44.0 81 29.0 82 23.5 83 12.5 84 8.5 85 10.5 86 8.0 87 6.0 88 4.5 89 3.5 90 2.0 91 0.5 92 0.5 93 1.0 94 2.0 95 2.0 96 1.5 97 1.5 98 1.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08927450688780784 2 0.10099825883641556 3 0.09780840406594632 4 0.1001282984444694 5 0.09664845687668477 6 0.07966351589106936 7 0.09171868132232322 8 0.08852882655185398 9 0.08297765071753091 10-11 0.08908808680381938 12-13 0.08823883975453861 14-15 0.09416285575683861 16-17 0.09107656769969631 18-19 0.09379001558886169 20-21 0.10437453369087325 22-23 0.11326055769432326 24-25 0.13703947507418482 26-27 0.14339847127245792 28-29 0.14292206439115407 30-31 0.1445169917763887 32-33 0.14010504978866176 34-35 0.13442959389834638 36-37 0.1380337155221233 38-39 0.13094975233056175 40-41 0.12678637045481944 42-43 0.12680708379748482 44-45 0.1175067929407271 46-47 0.13701876173151945 48-49 0.15576433684369256 50-51 0.16595530143506182 52-53 0.1748827521238426 54-55 0.17005654328280795 56-57 0.13981506299134638 58-59 0.13045263210659253 60-61 0.12707635725213484 62-63 0.12380364911100405 64-65 0.10990499618253094 66-67 0.1230372554323848 68-69 0.12055165431253864 70-71 0.12927197157466558 72-73 0.15775281773956948 74-75 0.17212787754934644 76-77 0.17624983273975797 78-79 0.1738056583052426 80-81 0.17906684734225028 82-83 0.1783625936916272 84-85 0.15499794316507334 86-87 0.15607503698367334 88-89 0.17749263329968104 90-91 0.27412037683370044 92-93 0.5607101859519624 94-95 0.17653981953707334 96-97 0.1891956719056234 98-99 0.2067398731432042 100-101 0.21382383633476573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2413903.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.15477607382669 #Duplication Level Percentage of deduplicated Percentage of total 1 72.01065783061603 34.676571027622686 2 13.459395568747684 12.962683594041998 3 5.648353310313517 8.159855665320155 4 2.845339541065505 5.480667538160568 5 1.6434971252829185 3.9571118022988414 6 1.0296780169421333 2.9750348600394214 7 0.707119094230427 2.383581314813535 8 0.4906266032772996 1.8900811373344453 9 0.3443841465475316 1.492536731433507 >10 1.6679716004265033 13.945901973970232 >50 0.0944896453459976 3.1289781928893743 >100 0.05428247926978163 4.821292584161305 >500 0.0016644941823886542 0.5577829066768732 >1k 0.002190123924195028 1.9362516904137193 >5k 2.6281487090340337E-4 1.0439762208084953 >10k+ 8.760495696780111E-5 0.5876927600146744 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13931 0.5771151533429472 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9107 0.377272823307316 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8773 0.36343631040683905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6867 0.2844770481663928 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4933 0.20435783873668495 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3986 0.16512676772844642 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3328 0.13786800878080022 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2760 0.11433765151292327 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.242800559923079E-4 0.0 0.0 0.0 0.0 6 1.242800559923079E-4 0.0 0.0 0.0 0.0 7 1.242800559923079E-4 0.0 0.0 0.0 0.0 8 1.242800559923079E-4 0.0 0.0 0.0 0.0 9 1.242800559923079E-4 0.0 0.0 0.0 0.0 10-11 1.242800559923079E-4 0.0 0.0 0.0 0.0 12-13 1.242800559923079E-4 0.0 0.0 0.0 0.0 14-15 2.071334266538465E-4 0.0 0.0 2.071334266538465E-5 0.0 16-17 2.071334266538465E-4 0.0 0.0 1.0356671332692325E-4 0.0 18-19 2.2784676931923115E-4 0.0 0.0 1.657067413230772E-4 4.14266853307693E-5 20-21 2.485601119846158E-4 0.0 0.0 2.071334266538465E-4 4.14266853307693E-5 22-23 3.314134826461544E-4 0.0 0.0 7.249669932884627E-4 4.14266853307693E-5 24-25 4.971202239692316E-4 0.0 0.0 0.0011806605319269249 4.14266853307693E-5 26-27 4.971202239692316E-4 0.0 0.0 0.0021334742945346187 4.14266853307693E-5 28-29 6.628269652923088E-4 0.0 0.0 0.005716882575646163 4.14266853307693E-5 30-31 7.456803359538474E-4 0.0 0.0 0.015493580313707716 4.14266853307693E-5 32-33 7.66393678619232E-4 0.0 0.0 0.036931889972380824 4.14266853307693E-5 34-35 8.078203639500013E-4 0.0 0.0 0.06680053009586549 4.14266853307693E-5 36-37 8.699603919461552E-4 0.0 0.0 0.11147921022510018 4.14266853307693E-5 38-39 9.113870772769246E-4 0.0 0.0 0.1795639675662195 4.14266853307693E-5 40-41 9.113870772769246E-4 0.0 0.0 0.25638975551213117 4.14266853307693E-5 42-43 9.942404479384632E-4 0.0 0.0 0.336239691487189 4.14266853307693E-5 44-45 0.0010770938186000017 0.0 0.0 0.4324531681679007 4.14266853307693E-5 46-47 0.0012220872172576942 0.0 0.0 0.5427931445464047 4.14266853307693E-5 48-49 0.0012428005599230789 0.0 0.0 0.6590364235845434 4.14266853307693E-5 50-51 0.0012428005599230789 0.0 0.0 0.7920575101816436 4.14266853307693E-5 52-53 0.0012428005599230789 0.0 0.0 0.923960076274813 4.14266853307693E-5 54-55 0.0012842272452538482 0.0 0.0 1.0653700666513939 4.14266853307693E-5 56-57 0.0013256539305846176 0.0 0.0 1.2332309956116712 4.14266853307693E-5 58-59 0.0013256539305846176 0.0 0.0 1.4167719249696447 4.14266853307693E-5 60-61 0.0013670806159153867 0.0 0.0 1.6198455364610758 4.14266853307693E-5 62-63 0.0013670806159153867 0.0 0.0 1.8297545510320838 4.14266853307693E-5 64-65 0.0013670806159153867 0.0 0.0 2.0375922313365535 4.14266853307693E-5 66-67 0.001408507301246156 0.0 0.0 2.278074139681669 4.14266853307693E-5 68-69 0.001408507301246156 0.0 0.0 2.5503717423608157 4.14266853307693E-5 70-71 0.001408507301246156 0.0 0.0 2.856867073780512 4.14266853307693E-5 72-73 0.001408507301246156 0.0 0.0 3.1684164608105627 4.14266853307693E-5 74-75 0.0014292206439115408 0.0 0.0 3.489556125494686 4.14266853307693E-5 76-77 0.0014499339865769254 0.0 0.0 3.8252572700725755 4.14266853307693E-5 78-79 0.0014499339865769254 0.0 0.0 4.216863726504338 4.14266853307693E-5 80-81 0.0014913606719076948 4.14266853307693E-5 0.0 4.646417026699084 4.14266853307693E-5 82-83 0.001532787357238464 6.214002799615395E-5 0.0 5.068492810191628 4.14266853307693E-5 84-85 0.0015742140425692333 8.28533706615386E-5 0.0 5.485203837933836 4.14266853307693E-5 86-87 0.0016156407279000026 8.28533706615386E-5 0.0 5.928490084315733 4.14266853307693E-5 88-89 0.0016156407279000026 8.28533706615386E-5 0.0 6.411380241873845 4.14266853307693E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11160 0.0 28.40231 1 TACATGG 11370 0.0 27.461536 2 ACATGGG 11575 0.0 25.49933 3 CATGGGA 8100 0.0 23.72725 4 GTATCAA 24230 0.0 22.481737 1 GAGTACT 10170 0.0 21.974718 12-13 ATGGGAG 3115 0.0 21.937233 5 AGTACTT 10450 0.0 21.681057 12-13 CGTATAG 180 5.2194955E-6 21.089138 1 AGAGTAC 16815 0.0 20.556826 10-11 TATCAAC 27415 0.0 19.61106 2 TCAACGC 27510 0.0 19.595896 4 ATCAACG 27605 0.0 19.57922 3 CAACGCA 27595 0.0 19.501143 5 AACGCAG 27825 0.0 19.489801 6 GTACTTT 11635 0.0 19.310364 14-15 CATGGGG 4160 0.0 19.278389 4 ACGCAGA 29965 0.0 17.749563 7 CGCAGAG 29925 0.0 17.536194 8 GGTATCA 18625 0.0 17.324232 1 >>END_MODULE