FastQCFastQC Report
Fri 27 May 2016
SRR939601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939601_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413903
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144440.5983670429176318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106220.4400342515834315No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT101270.41952804234470065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81040.33572185792055437No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52230.21637157748260805No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42360.17548343906113875No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36870.1527401888145464No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28150.11661611920611559No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27420.11359197117696941No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24720.10240676613766171No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24550.10170251248703864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG113600.027.3376121
TACATGG114750.026.6480562
ACATGGG116300.024.7951983
GTATCAA219850.024.6773091
AGTACTT114850.021.67846912-13
CATGGGA83950.021.573814
CATGGGG39100.021.295284
GAGTACT109600.021.25958812-13
TCAACGC255350.021.0938824
AGAGTAC176000.021.08619310-11
ATCAACG256450.020.985063
AACGCAG256650.020.9583446
CAACGCA259450.020.7605425
TATCAAC263300.020.4935632
GGTATCA160550.019.6949061
CGCAGAG275850.019.2611568
GTACTTT121900.019.21078914-15
ACGCAGA279550.019.0902487
TACTTTT136200.018.22852914-15
GCAGAGT280350.018.164049
Produced by FastQC (version 0.11.5)