FastQCFastQC Report
Fri 27 May 2016
SRR939600_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939600_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255327
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52730.23380201629298103No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36190.1604645357413803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36070.15993246212190074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26380.11696751734892546No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88700.032.8382841
TACATGG106150.026.569382
GTATCAA195700.026.2841221
ACATGGG107600.025.289893
GAGTACT91700.022.43933712-13
CATGGGA74550.022.4307654
GGTATCA152950.021.6100351
AGTACTT99400.021.19961212-13
TATCAAC236400.020.5833872
GTACTTT102800.020.019314-15
AGAGTAC160050.019.94737210-11
GTATACC3156.1056577E-419.6741351
ATCAACG253550.019.1729323
CAACGCA255900.019.0885335
ATGGGAT32500.019.02355
TCAACGC256150.018.9946524
AACGCAG258650.018.8490876
CATGGGG35850.018.7105964
GAGTACA84950.018.3598231
ACGCAGA267850.017.88497
Produced by FastQC (version 0.11.5)