FastQCFastQC Report
Fri 27 May 2016
SRR939600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939600_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255327
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134010.5941932145538097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99960.44321732502648176No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93750.415682515218414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75420.3344082698429097No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49400.21903697335242295No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39950.17713617581840682No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34720.15394663390275556No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT26470.11736657256353512No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26100.11572601223680645No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23100.10242417174981722No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22960.10180341919375771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG99700.027.2071
TACATGG100950.026.4442582
ACATGGG102550.025.2004533
CGCATAG4250.022.90608466-67
CATGGGA73800.022.1736184
GTATCAA215600.021.9024811
GAGTACT99800.021.4406712-13
AGTACTT102600.020.99335712-13
AGAGTAC158000.020.0752710-11
GGTATCA163450.019.7414571
ATGGGAG31450.019.3433885
GTACTTT110950.019.07357214-15
ATCAACG247250.018.9016973
TCAACGC247750.018.8825864
ACGCATA6350.018.77963864-65
AACGCAG248750.018.776476
CATGGGG34200.018.7532394
CAACGCA249300.018.746275
TATCAAC250950.018.6442852
AACGCAT7050.017.26019964-65
Produced by FastQC (version 0.11.5)